LOCUS       DACEDD010000001       101341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEDD010000001 DACEDD010000000
VERSION     DACEDD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.001"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..970
                     /locus_tag="IWO45_000001"
     CDS             83..970
                     /locus_tag="IWO45_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0331515.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IWO45_000002"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IWO45_000002"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0331516.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IWO45_000003"
     CDS             2272..2718
                     /locus_tag="IWO45_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0331517.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IWO45_000004"
     CDS             2991..4343
                     /locus_tag="IWO45_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0331518.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IWO45_000005"
     CDS             4514..4801
                     /locus_tag="IWO45_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331519.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IWO45_000006"
     CDS             5027..5470
                     /locus_tag="IWO45_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0331520.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IWO45_000007"
     CDS             complement(5476..6660)
                     /locus_tag="IWO45_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0331521.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IWO45_000008"
     CDS             6928..7626
                     /locus_tag="IWO45_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0331522.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IWO45_000009"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IWO45_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0331523.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IWO45_000010"
     CDS             complement(8436..8969)
                     /locus_tag="IWO45_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331524.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IWO45_000011"
     CDS             complement(9266..11647)
                     /locus_tag="IWO45_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0331525.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IWO45_000012"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IWO45_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0331526.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IWO45_000013"
     CDS             12348..12887
                     /locus_tag="IWO45_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0331527.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IWO45_000014"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IWO45_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0331528.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IWO45_000015"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO45_000015"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IWO45_000016"
     CDS             13994..15160
                     /locus_tag="IWO45_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0331529.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IWO45_000017"
     CDS             15206..16702
                     /locus_tag="IWO45_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0331530.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IWO45_000018"
     CDS             16783..17466
                     /locus_tag="IWO45_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0331531.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IWO45_000019"
     CDS             18111..19301
                     /locus_tag="IWO45_000019"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0331532.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IWO45_000020"
     CDS             19403..20356
                     /locus_tag="IWO45_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0331533.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IWO45_000021"
     CDS             20425..21774
                     /locus_tag="IWO45_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0331534.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IWO45_000022"
     CDS             complement(21957..22280)
                     /locus_tag="IWO45_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331535.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IWO45_000023"
     CDS             22409..23572
                     /locus_tag="IWO45_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331536.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IWO45_000024"
     CDS             23642..24991
                     /locus_tag="IWO45_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0331537.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IWO45_000025"
     CDS             complement(25036..25467)
                     /locus_tag="IWO45_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0331538.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IWO45_000026"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IWO45_000026"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0331539.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IWO45_000027"
     CDS             28697..29293
                     /locus_tag="IWO45_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0331540.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IWO45_000028"
     CDS             29415..30563
                     /locus_tag="IWO45_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0331541.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IWO45_000029"
     CDS             complement(30601..31014)
                     /locus_tag="IWO45_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0331542.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IWO45_000030"
     CDS             31093..31524
                     /locus_tag="IWO45_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0331543.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IWO45_000031"
     CDS             complement(31623..31856)
                     /locus_tag="IWO45_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0331544.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IWO45_000032"
     CDS             32039..33709
                     /locus_tag="IWO45_000032"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0331545.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IWO45_000033"
     CDS             33856..34242
                     /locus_tag="IWO45_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331546.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IWO45_000034"
     CDS             34229..34927
                     /locus_tag="IWO45_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0331547.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IWO45_000035"
     CDS             34932..35396
                     /locus_tag="IWO45_000035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0331548.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IWO45_000036"
     CDS             35393..36304
                     /locus_tag="IWO45_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0331549.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IWO45_000037"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IWO45_000037"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0331550.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IWO45_000038"
     CDS             37092..38375
                     /locus_tag="IWO45_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0331551.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IWO45_000039"
     CDS             38388..39314
                     /locus_tag="IWO45_000039"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0331552.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IWO45_000040"
     CDS             39333..40613
                     /locus_tag="IWO45_000040"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0331553.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IWO45_000041"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IWO45_000041"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0331554.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IWO45_000042"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IWO45_000042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0331555.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IWO45_000043"
     CDS             44938..45714
                     /locus_tag="IWO45_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0331556.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IWO45_000044"
     CDS             45711..46643
                     /locus_tag="IWO45_000044"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0331557.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IWO45_000045"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IWO45_000045"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0331558.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IWO45_000046"
     CDS             47363..47992
                     /locus_tag="IWO45_000046"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0331559.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IWO45_000047"
     CDS             47980..48294
                     /locus_tag="IWO45_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331560.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IWO45_000048"
     CDS             48332..48973
                     /locus_tag="IWO45_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0331561.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IWO45_000049"
     CDS             49004..49708
                     /locus_tag="IWO45_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0331562.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IWO45_000050"
     CDS             49705..50607
                     /locus_tag="IWO45_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0331563.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IWO45_000051"
     CDS             complement(50765..52471)
                     /locus_tag="IWO45_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0331564.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IWO45_000052"
     CDS             52661..53284
                     /locus_tag="IWO45_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331565.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="IWO45_000053"
     CDS             53734..54783
                     /locus_tag="IWO45_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0331566.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="IWO45_000054"
     CDS             54780..55661
                     /locus_tag="IWO45_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0331567.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="IWO45_000055"
     CDS             55664..56434
                     /locus_tag="IWO45_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0331568.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="IWO45_000056"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="IWO45_000056"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0331569.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="IWO45_000057"
     CDS             57745..58908
                     /locus_tag="IWO45_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0331570.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="IWO45_000058"
     CDS             59025..59435
                     /locus_tag="IWO45_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0331571.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="IWO45_000059"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="IWO45_000059"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0331572.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61975..62292
                     /locus_tag="IWO45_000060"
     CDS             61975..62292
                     /locus_tag="IWO45_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331573.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62452..62688
                     /locus_tag="IWO45_000061"
     CDS             62452..62688
                     /locus_tag="IWO45_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0331574.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63084
                     /locus_tag="IWO45_000062"
                     /pseudo
     CDS             62685..63084
                     /locus_tag="IWO45_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(63751..64695)
                     /locus_tag="IWO45_000063"
     CDS             complement(63751..64695)
                     /locus_tag="IWO45_000063"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0331575.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64991..65935)
                     /locus_tag="IWO45_000064"
     CDS             complement(64991..65935)
                     /locus_tag="IWO45_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0331576.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66156..67370
                     /locus_tag="IWO45_000065"
     CDS             66156..67370
                     /locus_tag="IWO45_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0331577.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67392..69407
                     /locus_tag="IWO45_000066"
     CDS             67392..69407
                     /locus_tag="IWO45_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0331578.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69490..70863)
                     /locus_tag="IWO45_000067"
     CDS             complement(69490..70863)
                     /locus_tag="IWO45_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0331579.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70889..71173)
                     /locus_tag="IWO45_000068"
     CDS             complement(70889..71173)
                     /locus_tag="IWO45_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0331580.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71175..73190)
                     /locus_tag="IWO45_000069"
     CDS             complement(71175..73190)
                     /locus_tag="IWO45_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0331581.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73369..73674)
                     /locus_tag="IWO45_000070"
     CDS             complement(73369..73674)
                     /locus_tag="IWO45_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0331582.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73763..74794)
                     /locus_tag="IWO45_000071"
     CDS             complement(73763..74794)
                     /locus_tag="IWO45_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0331583.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75001..75876
                     /locus_tag="IWO45_000072"
     CDS             75001..75876
                     /locus_tag="IWO45_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0331584.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76028..76870)
                     /locus_tag="IWO45_000073"
     CDS             complement(76028..76870)
                     /locus_tag="IWO45_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0331585.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76962..78839)
                     /locus_tag="IWO45_000074"
     CDS             complement(76962..78839)
                     /locus_tag="IWO45_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0331586.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78836..80257)
                     /locus_tag="IWO45_000075"
     CDS             complement(78836..80257)
                     /locus_tag="IWO45_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0331587.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80260..83649)
                     /locus_tag="IWO45_000076"
     CDS             complement(80260..83649)
                     /locus_tag="IWO45_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0331588.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83906..85297
                     /locus_tag="IWO45_000077"
     CDS             83906..85297
                     /locus_tag="IWO45_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0331589.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85370..86017)
                     /locus_tag="IWO45_000078"
     CDS             complement(85370..86017)
                     /locus_tag="IWO45_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0331590.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86025..86711)
                     /locus_tag="IWO45_000079"
     CDS             complement(86025..86711)
                     /locus_tag="IWO45_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0331591.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86717..87790)
                     /locus_tag="IWO45_000080"
     CDS             complement(86717..87790)
                     /locus_tag="IWO45_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0331592.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88012..88686
                     /locus_tag="IWO45_000081"
     CDS             88012..88686
                     /locus_tag="IWO45_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0331593.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88691..90031
                     /locus_tag="IWO45_000082"
     CDS             88691..90031
                     /locus_tag="IWO45_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0331594.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90180..91166)
                     /locus_tag="IWO45_000083"
     CDS             complement(90180..91166)
                     /locus_tag="IWO45_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0331595.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91890..92921
                     /locus_tag="IWO45_000084"
     CDS             91890..92921
                     /locus_tag="IWO45_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0331596.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93030..94562
                     /locus_tag="IWO45_000085"
     CDS             93030..94562
                     /locus_tag="IWO45_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0331597.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            94701..95513
                     /locus_tag="IWO45_000086"
     CDS             94701..95513
                     /locus_tag="IWO45_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0331598.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            95503..95988
                     /locus_tag="IWO45_000087"
     CDS             95503..95988
                     /locus_tag="IWO45_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0331599.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            96011..96556
                     /locus_tag="IWO45_000088"
     CDS             96011..96556
                     /locus_tag="IWO45_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0331600.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(96629..98272)
                     /locus_tag="IWO45_000089"
     CDS             complement(96629..98272)
                     /locus_tag="IWO45_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0331601.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(98401..100629)
                     /locus_tag="IWO45_000090"
     CDS             complement(98401..100629)
                     /locus_tag="IWO45_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0331602.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(100662..101141)
                     /locus_tag="IWO45_000091"
     CDS             complement(100662..101141)
                     /locus_tag="IWO45_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331603.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        32047 a        18135 c        20470 g        30689 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtggaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaagaacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacc gtcactcgcc aggcgaaatg
    62761 atcggtgtaa aagatgcaaa tgcattagat atggccgtca aacagccttc acaagcagta
    62821 ttcaatcaag aactctatcc tgaaatctat gaaaaagcgg cgattttagc aattaattta
    62881 gctaaaaaac atccttttca taatggcaat aaacgaactg ctcttgttgc catgttgttg
    62941 tttcttcaat tgaataattg tccagttgct ttttctcgtc aagaagctgt tgattttatc
    63001 ataatgatta ccactagttc attggatttc gattcactaa agtccaagat aaccagttat
    63061 ttacgacaaa ccgcaataaa atgaacgaat taaaaacttt atttctcaat tagatcagca
    63121 tgtcattaag gaatatgctg atttttttat gtttaatcat acaaaatagt tttttcataa
    63181 aaatatggaa aatatggatt aattttgaaa taattgattt aaaaggaata caaaagagtg
    63241 aatagtagga aatagtggtt tagaactagt gggctgattt tttatatatt ttagtttaca
    63301 taatatatat tatacaaagt agatttaaaa aatgataaac ctctctcgaa cggtttttta
    63361 tcaaaaatcg tcatttttcc atcaaaatct gcttatgcaa aattataaaa aaattttgca
    63421 gtttttctta ctttttttgt cgtttaattg aatgataaaa accagtttct ttattcagtt
    63481 aatcaaatat gaacgaatta gatatgaatc attgtatttg ccgttgcgga aggatttttt
    63541 aattaaccac agataaacat tgataacgat tcgcttattg taatcaacag attaaatttc
    63601 atatgttcat atgtacggat acattttcgg accgattaat tttaaccatt ctgtttattt
    63661 gcttacctat tagatatcaa taaaaaactg cttaacagct atttcagctg ttaagcagtt
    63721 ttaggatttt tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa
    63781 agcacttgtt gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac
    63841 ttcacgaaca ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc
    63901 gtattcacca gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc
    63961 ttttacgata cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat
    64021 gatctcataa gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat
    64081 acgatggtct ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca
    64141 tgcagcaact tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc
    64201 aacttttaat ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt
    64261 accgataaca cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc
    64321 tactgggttt gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac
    64381 gatttggcgc atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt
    64441 ttggttgata ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc
    64501 tgaccataca gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc
    64561 cccttctgct ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt
    64621 gatcattgca taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg
    64681 acttgttttt ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa
    64741 gtatagtctt gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt
    64801 ttgatggaaa cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata
    64861 tcccctgata atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca
    64921 acgatataat aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa
    64981 gattttcttc ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga
    65041 ataatttgaa taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg
    65101 aaaaactcgt ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt
    65161 cttgaaagga agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc
    65221 caaaatgagc tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta
    65281 atactctaag catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta
    65341 ataaatgagt caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat
    65401 gactgtatgt atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg
    65461 tttttccgcc gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta
    65521 tcaaaaaaga agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata
    65581 aggtttcttc ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc
    65641 tttgagtagc ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc
    65701 cattaataat ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg
    65761 tatgctcggg aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa
    65821 atagatcccc tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat
    65881 tgatattcat tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa
    65941 atgtctaatt cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt
    66001 attagttcac gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc
    66061 tatggttggg acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta
    66121 tatttttgat aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga
    66181 aattttctct gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa
    66241 ttcccagtat gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga
    66301 ctgctccctc ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa
    66361 tcggacaaaa acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc
    66421 ccctcttctc acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct
    66481 ttggattgat caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac
    66541 gagcctctat gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat
    66601 ttgttgcagg acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca
    66661 gcttacctat tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc
    66721 aatcgcatgg cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt
    66781 atggtatttt tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca
    66841 cagtcctgac gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca
    66901 tgcaattggc aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc
    66961 gctatatgct tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg
    67021 tgataaacct gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat
    67081 tcattcctta cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca
    67141 atacttcaat cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc
    67201 tctttctctc ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta
    67261 tcttgctgtt gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga
    67321 cagtgataag agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga
    67381 ggtcgtgtaa aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact
    67441 ttgagatccg ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc
    67501 cagcgtactg gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc
    67561 ttgatcaacc ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg
    67621 gacaaaaaca tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta
    67681 tctttgtagg acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat
    67741 cagatattgg ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc
    67801 tttatgtcga tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc
    67861 cagtcactga aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg
    67921 gctgtgtaga actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag
    67981 agtccgatgt agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa
    68041 gaaaaatgaa agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg
    68101 gaatggttca acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt
    68161 caagcgattc ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat
    68221 gtagtgattt gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg
    68281 cgtatcaatc attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa
    68341 cgatattagc attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact
    68401 attcgatctt gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat
    68461 ttttacgcat cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga
    68521 atgaattagg gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa
    68581 tggataaaga aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca
    68641 tgatcacttg cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc
    68701 atctctatca agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg
    68761 aagaacttgg cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg
    68821 cgaatatttt cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa
    68881 agaatgtctt attgaatgac actatcacac agattactac tccatatttc aaattctatg
    68941 aacaagatgc tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt
    69001 attggggagg gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc
    69061 aaacaggaaa tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg
    69121 catggggagc aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa
    69181 agccaggtta tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt
    69241 gtacgctacc tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga
    69301 ttacttctga tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag
    69361 caaaacagcc acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc
    69421 ttgcgacata tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt
    69481 gaagtttgct taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa
    69541 gctagataat acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc
    69601 gtattctcct agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt
    69661 agaagaagcg attttaagat ctcctaatac tggaaggata aataatggca gccaactaat
    69721 caataagcta ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc
    69781 gaagacacca gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt
    69841 agtttgcaag cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa
    69901 gccaattaat acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt
    69961 agaatcgggt acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac
    70021 cattcgaacc ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag
    70081 tgcaaagatt acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc
    70141 taacaaagca agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc
    70201 tcgcaaaaag ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc
    70261 aaaaaacttt ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc
    70321 aacttgattg tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt
    70381 taatagtgtt ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc
    70441 cgcgcttact aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag
    70501 aaaaatgaat ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag
    70561 catgatcaaa gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt
    70621 gctaggaact actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag
    70681 tgaattactg attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat
    70741 agttccttgg atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat
    70801 caaagaaacg aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc
    70861 cattgatagt tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt
    70921 cttttacttc ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag
    70981 aagttagcgt atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata
    71041 cacttccaac atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa
    71101 gtgcttgttt gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg
    71161 caaaaatttt catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt
    71221 gatgcgtgta ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc
    71281 aaagcccgaa gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga
    71341 tgggctggat tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact
    71401 tcgtttacgc catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata
    71461 aacgcaccat actgctccac gcgctcgatc atagctttga tataactttc ttctatttta
    71521 ttttctgata atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga
    71581 tccgataatt ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc
    71641 ttcttttcta aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct
    71701 acgccttctt ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc
    71761 attgaaaatc ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc
    71821 aagtcagtca tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga
    71881 ggtacagtag aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct
    71941 gaattagtct ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc
    72001 aatgaagagc taatgccact tggacagacg atcacggctc ttatttttct atttctttct
    72061 tctgcgtccg cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct
    72121 tcagggatgc tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg
    72181 tagatggaag catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa
    72241 taggaaatac gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga
    72301 aacgaagcaa attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat
    72361 tccagcaaaa aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa
    72421 agagagaaaa atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact
    72481 tgtcgactaa tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc
    72541 atggatgttt ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt
    72601 cgatttggtg cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact
    72661 ttggcgtaat aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca
    72721 agtgactgca tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac
    72781 cctagcagcc taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat
    72841 tcatttctta tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct
    72901 tgaaaatgaa aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct
    72961 cttcgctctt gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc
    73021 cagtcgattg ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa
    73081 ggagttaatt gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct
    73141 agactgattt ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc
    73201 acctcctact tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact
    73261 tacagttgga ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa
    73321 aagcaggtga ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc
    73381 atgtttattt tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc
    73441 gatatctttt tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga
    73501 accagataaa gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc
    73561 ttctttgtct tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt
    73621 aggggccatt gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt
    73681 tcctcctagt agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac
    73741 aaacccgatt cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc
    73801 cttcttccaa aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc
    73861 cattaactaa tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa
    73921 ttactttttc ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt
    73981 cgggcaaatc gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt
    74041 cttgtcgatc ttttgtttgt ttcacgaaca tccctttttt tccgtaaacg acgaaacttg
    74101 gacgttcttt gatgcggaaa taactggatt ttatcgaaat tttcatattt ccatagaaaa
    74161 aatccagatc gaagtaatca ttcattcgtc ctttccctaa aagttgacgt acatcatagc
    74221 tgatatcatc tggttcgccg aaataagaaa gaacttgatc tactgtatga caagcatgtc
    74281 catataaata gctgttcgca gtagaaaatt catgcgtatt ttctggtact tccggtctga
    74341 aataatcata atgcatttct acttctaaaa tatcccctaa ttttccgctt tcgattactt
    74401 tttggactgt caagaagtca gaatcgaacc gtctgttttg gtaacattgg ataaatagac
    74461 ctttttcttt tgctaatgca aataactctt ctgcttcctt tgacgtttct gcaaaaggtt
    74521 tctctactag tacattctta ccatgttcga gtgccatttt tccatattca taatgaaatt
    74581 gactaggtgt agtgatgaca actaattgga tctcaggatc atcccaaaga tcagacagct
    74641 gatcagtata ttcgatgtct tcgaatgttt tccacactgg ttcagatggg gaaaaaattt
    74701 ttttgatatt gattttgttc gtaagttttc ttgaaaaagg caaatgatag cgatttgtac
    74761 tctttccatt gccaagataa gcgattgtaa gcatgagtgg tgcctccttt tattgatgtt
    74821 ttcagtatat cctagaaaag cgcagaaagt atttataata gctattctgg taaaaaagtt
    74881 cagaaaaaca cttgttatca ttcggtttgg tgaaattaat gttcaaagta aggataaaaa
    74941 tcaaaaagat agtagcggaa ccaagtgttt atcagtaaga tagaatttgg aggtcagatc
    75001 atgcttattg aagaaaaact aacaaaacaa gagttgttta ccacgacaga gaaacggatt
    75061 gtcgattaca tccgtaggaa tatcgaagct gcagtctaca tgacgattga agaattagca
    75121 aaagctacgt atacttccca ttcagctatc attcgtttat gcaaaaaaat gggattcagc
    75181 ggatttaaag aattccgttt cgagctgggg cgggaagttc atcaattgat tgctcagttc
    75241 aatcaaacga ttgatcctaa ttttcctttt tcaagtgaag atagcactca gcaaattgca
    75301 aagaaaatgg cggatttatc gatccagtca ataaaaaaag cacaattgca gattgaacat
    75361 gaagatttag atcaaatcgg taaagtattg aacaaggcac agcgaatctt tctttttgca
    75421 aaaggtgatt ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa gttgaataag
    75481 tttttgatca tggctgaaga atacagtgat tcttcttgga atgcagccaa tttgactagc
    75541 gaggattgcg ccatttttat tagctacagc ggtcgcatcc atcattatga acgaataatg
    75601 acctatttga agcatgtaga ggcccctacc ttgctgatta ccggaaatca gcattcagaa
    75661 atggccaaac aggctgatat gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa
    75721 gtagcgactt tctcctctca aattgccttc gattacgtat taaatacgtt attctcagtg
    75781 atatattctc aaaattttga agagaatatc cgcaacttga aaaacaaaca agaattgatg
    75841 caaaaaggcc ttcttaaaga atatcggaaa acttaagaaa agatcctcat ttagtaataa
    75901 gaaagatagt gtttgatagg ctctctttga tatcatcata aaaacaggct gaagttttct
    75961 ttttcaaacc tcagcctgtt tgtttacgga acgattcgaa tagatatatt atagaaatga
    76021 ggaatagcta acttctgttt cttattttga acgattgacg aaaaagatat ataagaagga
    76081 ggaaaatcag tatacatgca gctaacaatc catacaggaa aaatcgtttg ttctcggata
    76141 ctttctgtac ttctttttga acttcttttg catgataagg aacccggtgc cctctgacaa
    76201 gtaagcgatg actgttgacc atataaggtg tacaagttag gagtgtcacg aaatcctgcc
    76261 ctttttcgat tctcagatct tttgtatcag tcggttctac cgtcttgatc tgatcgactt
    76321 gataagcgag tgtccgtcca ttgatttcga tatagaaaga atcacctaaa gtcagtttcg
    76381 gtaagtctgt gaacaacttt gcttgaggca gtcctcgatg gctggaaata acggcatgtg
    76441 tattttctcc ccccgtagga taagacgttc cttccaataa tgaggcacct ttttctagca
    76501 gcacagtatt cgtttcatca aaaattggca aatggacatt gatctcaggg atcgtcaaaa
    76561 taccgatcgt atgtttctca aaataggttc tatccttttt cgcaggttct tttttcttag
    76621 taaagggatc tgcacctgga tttccacctt cttttgctaa ttgctggttt ttctttgtca
    76681 tgttttcttg gattttagcc atgacttctt cattttcttt gaccgcttgt tgctgataat
    76741 gagagatgat ctgctgatcc agataattat tcagcgcgtc gctaacaaat ggataagcta
    76801 gtatccctat tcctgagaga agcaagaaaa gcatcagcag atctagaagt ttccttttct
    76861 ttggtttcat ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa aaggagcatt
    76921 tgcgtaatcc gctgctcctt tttccaattc ttgcaaaaag actaaatctg actgtgcttg
    76981 cgtctagcaa agtacagtcc agcaatcagt agaaggacta ctcctgcacc gatatacaca
    77041 tagattccct taccgcctgt tgaaggaagt gtacctttgt gtttatttgg tattttttca
    77101 ggagaaatca actgtcctgc tgttacataa gattgttcat cgatagtaaa tgctacacgg
    77161 tttgttaatg gaacatattt ttctggcgct ttcgtttctt ccagatagta cgtgccatat
    77221 tttagacctg tcacatcgat taaaccgtta cttgtggttg taaaaaccgt tgctgattct
    77281 ttcgccgata cccagctgac ggcttttgtg gatgggtcga tcgataaata tttcgctgtg
    77341 tcactatctt gatcacgaac gacgaattct gctccagcaa gtgtttggtc tgatgtaacg
    77401 tcaccatcta ctttaacaaa tcgtttgccc ccagtaacga catcgactga cggtggtgtt
    77461 tgatcatttg tatgcccgtt atcgacattt gcttggttag aaaatccttt ggttggatct
    77521 gctttttcgt tcaaatgcat atagtaaacg aatttcaatg taccgccagg agttaatgaa
    77581 ggaatataat tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt gacagaataa
    77641 tttactgggt cgatttcttt atttccatca tataaggcat aagcatacgt tcctgaagaa
    77701 tcattatcaa atgttaaagc agcgtcatga gtatcgataa gtttgaatgt tgtgtacttg
    77761 ttttgcgttc cttctttatc agcgatacct aatgggatat tgacagaaat ctcatattga
    77821 attttttcac cgattgcgac atcttttcca ttcaattgag gtgttgtttt atcaacttta
    77881 gacgtatcat ttttgatggt cttttctact ggtgtttggc tatttgccag gatctcaaaa
    77941 ggcgtgattg tttgcttttc aatcatttcc gcattatcag gagcttttac ttcttctaaa
    78001 ttatattttc caacttctaa atgattaacg atcaactggc ctttttcaat agatgcctcg
    78061 gcaaagtcat tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt ggctttggtt
    78121 tgatcagttg tccaagtgta caatccatct gtgacacttt ggatgtattt tttgacgctt
    78181 ggtgttcctt cttctttaga aataataaat tctgctccat ttagtgcttc gttttcggca
    78241 gtaccgattt ttgtaacttt caacgttcca tcattaccga ctgtattttt agggtagaga
    78301 tggatagtat ctagttcttc tgtcccgtat ttataagagc catctgcttg tttgatcatc
    78361 tcatatacag ggaaagctaa aaccatgttt gcggcagctg acactccgtc ttttggttct
    78421 tctttgatcg tgtagactgc atctttccca ttttgttttt taggtaaaga taaagtgaca
    78481 tttccatcag catctgtcgt tcctgaagca actggtgtac caggagtcaa agactggact
    78541 gcttgttttg ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa ttcttgagtg
    78601 acgttataaa ctgaaaatga aatatcggct aatccttggt attgatcgaa ttcgctcatt
    78661 tctttcccgc tgttttggat taaaggatcg ggtaaatcag tcattttctt tttatgaagg
    78721 atgacttgag ctgctgtctc ctctgcaaac actttttttg cgccgaagct gtttgtgagt
    78781 aatggtaaaa taaggaaaag gactcctaac ataacgttta tttttttatg atttttcatt
    78841 ttttcactcc tgttcttttt ttgataaaca accagattgc aaagcttgcg aataccatac
    78901 caacaacgcc taaaagtgta tttttcgttt cgtttgtttt tggaagaaat ggacggctat
    78961 tttctttttt attgactact ttcaatatac tgtccgcctc tttatcctca gaaagaaaag
    79021 ttgaaatggt aaacggaatt tctgtttcac ttaaaatata accagaaggt gcttcgatct
    79081 cttttaatcg ataacttcct gttttcaacc cggaaattga aaaagcgcca tttttatcgg
    79141 aaatcaggtg aagcgcatct gattcgttct ttgtccactt gtatccgttg gctgtttgct
    79201 ggaggtaatt tcctgctttg tttttaataa ggaatactgc gccagctaat cgtttatctg
    79261 gtttatttgt ctcgattttg acaaattgct tactccctgt atatacagag actttctttg
    79321 tactgacacc aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg ttcgcctgtg
    79381 ctgtactgct tagctgcatc tgataagaaa tggtcagttt ttttccagca tatttttcca
    79441 acgaagggat cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg acaaagcctt
    79501 ctgtcagctt ttctccattc gatgaaatcg ttaaactatt aggtaaaaac gttaatgctt
    79561 catccgcact atctgacaat tcgaactttt gatagtcaag gatatttaca ggaattgtcg
    79621 tatgcaagga ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc tcttctaata
    79681 ccttctcaaa aggtggatca gggtagtcgt tttcttcatt tttaggatac aaatggatag
    79741 ttgaaagttt ttgattgttt tgtccatgta caggaagaac aactaccata ttctctgcct
    79801 tttctttgac gacttctggt actttggatt caatgaaaag atacgcttta tctctttttt
    79861 cttgatcttt agcggccaga gaaaaagaag cgataccatc ttcgttgtta agagttgtag
    79921 ttgttgcttc tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc tctgcttgtg
    79981 cttcttctac cgttttgccc ttttcccgta gatggtaaaa agaatctgtg acatcataaa
    80041 cttggaatgt gacaccattt aatcctcgat acgtttgtaa taaagctttt tcttgtccgt
    80101 catttggatg atttttcggc agttgcccgt ttgggaaaag caatttgtgt aaagtaatct
    80161 gaaccatttc tgtttctgtt tctgttgcta cctgagtaaa agctggttta aatagtaaca
    80221 agaagagaca catactaagc caaccaagtt ttttcatatt tagctgcccc ctctttcttt
    80281 tttcaaaaaa agataccaaa tagaaaacgc acaaccaatc atccctacta gatagattcc
    80341 taaacggcca attcctcccg tttcaggtaa tggtactttt gcctgattcg tgatgtctaa
    80401 agaaatctgg ttgtttttgg ctcctggtga cagaacagat tcatgatctt gtccatccac
    80461 ttgaattgac ccatcttcgt gtataactat agtaactggc tcttttagac cttggtatcc
    80521 ttctggtgta aaagtttcgg tcagcgtata agttccaggt tttaaatttt cgaatagaaa
    80581 ggtatctgtt tcttgtccat cttttggcga ttctaagtcc atttctggtc cctgcagtct
    80641 gaattttgct ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg ttagatcaaa
    80701 cgcttttagt cgattttgat gagtgagcgt ccatacatta tttgtcatct cggtattttt
    80761 tcccgtgtaa atgatttttc catatttatc gatttggaat tcataatttc ccggcgaagt
    80821 atcatatccg gcaggaccag ctgtttcaac gactcgatat ttaccaggtg tgagtgaatc
    80881 aaaactggca agaccttttt cattcgttgt ttggctgtca attggctgat aagtaccatt
    80941 tgcttctttt ttctgtaggg aaaatgctgc tcctttaaga tttatctctt tgtccgtccc
    81001 ttgcatcgca tacttacgta ctgctacagg aacttcaaca aaaggatttt caatagtcaa
    81061 ctggatcgta tcatcggaag tagtgactgt ttttccatca atggttaccg taccatcaga
    81121 agcgatcttg atttcccaag tagtcttttt gcttaatcca tgcccttctg gagcttttgt
    81181 ttctgtcagc gtataggtca tttctttttg caatttgaca ttttctggaa gagaataggt
    81241 tccgtcgcca tgatctgtta gtaaagtatc aatagaatct cctgttaatt tgaaaacagc
    81301 gccaataaga tccttttcac ctgtagagga atttttcgtt attttcaagt ttaacggtgt
    81361 gaattgttta gtatttttcc atgtcattgc atctatttgt tgagaatcgt atcctggtac
    81421 aggtaattct ttgattgttt ggtaactgaa tgcttgacct tgattattgt attgaggtag
    81481 tgataatatc tcttgataac tttctccgct atttgcagat aatttgtcaa tgtctgcacg
    81541 ttcccacgta tttgttgtat ctttagctgg tttaatgatt cgaatatatc cgtttttcca
    81601 agctgcagca tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat ctggacgatc
    81661 agctagattg ttgtcaaatt cttcccataa ctttttgatg tgaagactga ctccgggagc
    81721 tttagcagat ggtataccga attcagctaa ttcattggta tcaatgtttg gctggaaagt
    81781 tgtcctgccg ttcatttgat accagaaatt tggatggaag tcctcatttt ctgtttggat
    81841 tctcacttgg taatggattt ggatttcttg gtcttttcct aaatagatct gattgctctt
    81901 gatggtattt ccttctatgg aaatagatgg agtgaccgtt gtaggacttg tccccacact
    81961 cttgactgaa agtgtaccag gctgataaat gaatggttct gcgattggat catttatttg
    82021 tccattgctt acagttgctg agatctgtac agcttttttg gctaggtatt cagagatatc
    82081 tgttgcatga tcagctgatt catagtaaag atcgcctttt tcatctgatg aaaccatttg
    82141 tcgcatacga gactctactt ctgcttttga gagaccagct gccggatcgc tttgaagttg
    82201 gatgccaaga ccatgtattt cgattcctcg ttctttgagt gccatcgctt ctccgatggt
    82261 tgcgataaac gtactgtcga ttcttctgga tagattgttt tggtcaggtg catcatagat
    82321 tcttgagatt agagaagtat ttcccggccg atcttgcgta ttagaaaact gagttccgta
    82381 ataattgctg cttgattgtg cgtgtacccg ctgtacttta taggaaaatg ttggtacacc
    82441 atccgtcagc aaaacgatca cttttttatg tccatttgga acggatagca tgcttcctgc
    82501 atctcttagt gctttttgtg taaaagtacc accatttgtc cgtgaaggtg taatggattt
    82561 tacttgattt ttcactgaat caaatgaacc catctgtact gcaccgttac tgtagctata
    82621 tccttcgctt gagtagccga catatcccat attgattttg tctgtgatac cgctatctgc
    82681 taaagtatcg acaaaacgat cgacaccaat ctttacttca ccgatccgat tattgtcgtt
    82741 catacttcct gaccagtcta cgaccaatac gagatcaaga ggcgtgatat ctttttgtac
    82801 atttccacga gcattcaaat aaacatcaaa caatccgggt gtacttgttt cctttgcata
    82861 ctttcgtatc gcatagtctg cttgattacc ggttcctcca tattcgatat acgaatgggt
    82921 ccgatcatta ggatctccat tccaagaatt tataccatcc cattgatttg ttccgttttt
    82981 attgccttga tgatcaagaa catttgtatt tcctgtaggt tgccaactgg ctttaggata
    83041 agtgccctga tcatctgttg tatattctgt aggaatcgtt tgattagatg gcacactcac
    83101 agctggcaag gataatgtag gcaatgtaaa tgggctgaca gaattttcgt tttcttttga
    83161 tgattcttct tctatttcgt ctggctttgg tactgacaca tctgtatctt tcttattttc
    83221 tgtctcatca ataatgtcat attctttatt atcgacgatc agcttgtttg cctggctgtg
    83281 gtaaagtgct ggatcgattg gaaaactcag atcgatagtt tgtttagtgt ttgatgagat
    83341 ttccaacgta attttcccgc tatttacctg ataagcatag ccatctcctt cttttctttc
    83401 cacatcactt aagtaatttt cgactagacc aatttctttt tctatctttt cagcactggt
    83461 gttattcgca tttagcttga tgttcagtgc tttttttcca gactgatccg aaatgacttc
    83521 agctgaaatt gtttctaaaa ctgttggatc atctgtagtg tctgctattg cagataatgg
    83581 cacaaagttt gataaactca atattatcag catgaaaata actttcagtt tcttccctgt
    83641 ggtcgtcatt tttcccctcc tttttctttt tttctgtttt gacgtgaata gtgaaaatta
    83701 ttaaaataaa caggcgttac ataattttat ttattttaat atgataatta ttatacgaag
    83761 ggcgctaaag ggaatcacac caattttcat accaattggt ttcatttatg aagttgattc
    83821 cttcgattaa tgagcgtttc attgaaagaa aagggttgag ggagaaaaac atatgatcag
    83881 gttgttggaa tgaaactata gaaagatgga aaaaatcatt cgaattctgt ccatttctga
    83941 caagtggatg actaaaaaag aacttgccaa acgaattggt agttcagaat ctacatttgt
    84001 aaggtatatc gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca
    84061 aaaattgggg tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga
    84121 aatgtcgcaa agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa
    84181 aacgattgag tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt
    84241 gaaacaatgc aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt
    84301 tcagttaact agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt
    84361 aagttactac ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat
    84421 tatggtcaag aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc
    84481 tttctttatt atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga
    84541 atggaaagct ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat
    84601 cttgaaaaat ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt
    84661 tgtcaatacg attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat
    84721 acgaaaaaat atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga
    84781 atatgttgtt caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac
    84841 gactcatttg acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg
    84901 tctatatgct ggatttcgtg cagaacttca attttattcc actctactga atcagaatct
    84961 gatttattat ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca
    85021 ttatacttct gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata
    85081 tattgaagaa tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc
    85141 tattactgaa gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa
    85201 tcaacccatt ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc
    85261 aaatgaagaa gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat
    85321 aaaccaccct gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc
    85381 cttcagcaat ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat
    85441 tatttgcaat gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac
    85501 taactgcttc ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt
    85561 tttcgttcat tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc
    85621 gctgggttaa caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc
    85681 actctaaata tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta
    85741 agatatcttg atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca
    85801 aatcttgaaa atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg
    85861 agactttgac ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc
    85921 tctgtttctt tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt
    85981 tatctctgat tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc
    86041 tcttcttctg tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata
    86101 cggacatcat gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt
    86161 aatttgacga cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag
    86221 atcaagcttg gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca
    86281 gaaaggtctt taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa
    86341 tcgatttttg ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc
    86401 tttaccgtta aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag
    86461 cgtaaatctg ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt
    86521 tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa
    86581 ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc
    86641 aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat
    86701 atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac
    86761 gtatcgaaaa caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc
    86821 attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag
    86881 caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag
    86941 atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct
    87001 tctgaagtgt catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa
    87061 agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga
    87121 atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg
    87181 aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc
    87241 catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat
    87301 aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg
    87361 tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg
    87421 ttttaaattc atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga
    87481 aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg
    87541 ctttctctcc tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata
    87601 cttgatttgc atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt
    87661 taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca
    87721 agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag
    87781 ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg
    87841 aatagtgtaa ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat
    87901 tgtctaagac atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt
    87961 atttatgctt tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa
    88021 ctattagtag tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca
    88081 atcggcgaga taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga
    88141 gtttatgatc ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta
    88201 gcaaaaatac ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt
    88261 ttggaagata agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc
    88321 tatcgagaag aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt
    88381 gatgaaaatg ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg
    88441 tataaagaac aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta
    88501 caaaataagg gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat
    88561 tctgatacga cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga
    88621 agtactggat tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg
    88681 gtgtgatgca atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa
    88741 tgtagcagca tttgctttta tcttttttct tttgggattc attactttac agatattaaa
    88801 ccaatctgca tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac
    88861 agtacaatta gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac
    88921 ggaacaaaac agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa
    88981 cactcaagtg atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg
    89041 gcgtttcaac caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa
    89101 tattgaatta aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa
    89161 agcggagacg aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat
    89221 caagaattca ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat
    89281 tctctctatt ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc
    89341 ttggagaaga caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac
    89401 gattattcaa aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga
    89461 atccgaaagc atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga
    89521 tttgctgaca atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa
    89581 tacacaagaa tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg
    89641 aaaagagttt attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat
    89701 ccatcaagtt ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa
    89761 gatcattttg gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg
    89821 cccttctatc agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca
    89881 atctcgggcg aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat
    89941 cgaacagcat catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa
    90001 agtccaattg ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa
    90061 aaatcttgtc acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg
    90121 gaaataagcc gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct
    90181 tacgattttt tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct
    90241 tctcggctaa catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca
    90301 acttcttcac gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata
    90361 gtaatcgtgt catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt
    90421 ttgcgttcga tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg
    90481 accacatgtt tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat
    90541 ttttcttctt ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca
    90601 gtcttgcttg tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt
    90661 gtcatattca atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga
    90721 tctgttccag tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct
    90781 tgtgtatcca ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca
    90841 aattttccat cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc
    90901 gtctcagaat cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg
    90961 tcttcttcac catccatccg attttcatct gtttcaacat ctataccaga caatccgtca
    91021 aatcctagtc tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac
    91081 ccttcgttac gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg
    91141 atgaccgtta aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg
    91201 tatattgtta cttataaatt tattctatag gagacgattg ttttctacaa atatttagat
    91261 tggaatttga ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat
    91321 attctaataa aatgtcattt gtttctatat gaatttttat gaaaaatttc atataatttc
    91381 cactcgttgt gatggtttaa tttatccaat ccatcaataa gcaaacaact tatataaaaa
    91441 aggattaatt ctcaatataa ttgctcctac catagaataa atcttcatgt aaagtaatga
    91501 agtaccaact atttctaata cttctttaag aattatccca acaatatcgt tagacaaacc
    91561 attattgaat aaatggagag ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa
    91621 taaaagaaag agcctcctgc cattagaacg aaccaaatcc atttcattgc tcatgaagta
    91681 accacatagc taaaaagagg ataaatcaat taaaagatca aaagaatgac tgcactaata
    91741 aaataactaa aaatgtatct tcttacttca tatcttttag ctagtactta gttaaaaatt
    91801 tgcttcctaa gatcaagaca aactgtctat cttcatttga tgaaatgcgg taaaatatag
    91861 aaaatgaaca ctattggagg gaatttgtaa tggacagcaa aactagaaaa caactgcaag
    91921 aaaagttaaa gcaacgtaca cacttgctcc cttccgtctt tcaacagttt ttagaagaaa
    91981 atcgttcttc cctttcatta ggcagtcgct atgaatacgc aaaagatttt catctgttta
    92041 ctgattattt gagaaaagta ttttctgaag aagtggatga tcagctgatt atccatttag
    92101 aaaaacaaga ttatcagaat ttcttagcta aaatctatcg acatactcgt tcgttccaaa
    92161 ctacagcgaa ccaagaaacg gagcaattgt ttgaaaatag ctattacggg atttcaagga
    92221 agcaatattc attaaatcat tttttacgat ttttgtatca aaaaggactg attgaagaag
    92281 agatttctct tttgggtaca agtttaccgg aaacaaccaa agcagctccg cgatatatcg
    92341 atgcagaact tttcaaagat tttgagcatt tgtttatccc gattgaaggc tttcaaacaa
    92401 gcagagaagc tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt actgccattc
    92461 ttctgtattg cggattaaag atccatgaag tagtagaact taaacgtaaa gatgtatctt
    92521 tgaccaagca gattcttaat ctgaatagaa aagaaaacag actgaataaa gtaccaatac
    92581 caaaagaagc gatgccattt ctggagaact attttaagtt aacagcacaa aatacggacg
    92641 aaaattcttt tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg actattcgcc
    92701 aaattttgag caaaattaca gtatataaaa acgtatcgcc agaactatgc agaaaaacat
    92761 atcgttatta tgcagaacta tatttagatg atgctgacgc ggtaaattat ttatgtggta
    92821 atcgatacct ctcatcccat tcttttcaag atatctggga aaagatggtt gatttttctt
    92881 atcacgaatt agcggcattc gttcgggttt cttccatata aaacaaatgt atgatctttt
    92941 tttcacaata aactatgtta ttatataaag gattatgtta cgattatctt gaatttgtat
    93001 attacttaac aaaagataat ggagtgtgaa tgatggagac cataacgtat tcccctttga
    93061 acttatttac gaattttcaa gaagctgcag ctaacttttc cgagaccccg attatctttg
    93121 atgagccact ttcagctttc ccagaattag gacttgaaac aacttacaaa aaatgctgcg
    93181 aaagtgtgtt gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag
    93241 ttattattta taagagttcc gcttttgaca cataccttct agctgtttct gtgtcttacc
    93301 ttggtgcagt gcctgtcatg acttcttatc atctaccaac aacaacaatg gaagtattta
    93361 tcgatcgttt agaagaccct ttcattttat ttgatgatga gacaaaaaag cgagtgagag
    93421 aaatttcaaa tggaacaaaa tcaaaacaga ttcctattct ttatttaatg gaacaacctg
    93481 ctttgtctgt ttctcaatca tttttagaca aaaatgaaat ttcttatatg acccatactt
    93541 ctggtacaac tggtatccct aaacttattt gtcattctgc tcattctatg ggatggcgga
    93601 cgaaatggca aaaaacagtt ttcacaaaaa tttcggaaaa gaaattaatc gcttttcaca
    93661 tctctccagt gcattccaga ttcaatattg gtatctcttc tttgatgtcc atgggatttc
    93721 ctatgatgcc tttagcaaac gcacaaagtt caaaagttgt tcatatgttg gaagctcacc
    93781 ggccaattgc actagagacc catccgaata actttgttca gtggcgtttt actgctaagg
    93841 agcatccaga agctttttcg ggtatccgat actatcactc cacttttgat gctataaaca
    93901 accagacaat gaagacattt ttgcgaacgt cgatggcaca ggatgcggta tttcttcaag
    93961 tatatggcca aagtgaatgc ggaccaatga ttcttaaggc tcatacacta gagagtttga
    94021 aaacaagtga tgcaagagat atgggcgttg gactggaaga catgacaagc gctcggatta
    94081 cagattcagt tgggaatgtc ctacctgaaa acacagatgg tcatatacaa ttactgtcta
    94141 aaggacgggc tttgacttat tacaaggaag atgcacggtt ccaggaaaat gtttatggcg
    94201 attggtggga tagcggagat tacggtttta tggataagca tggccaccta tttttgaaag
    94261 atcggcaagt ggatttgatc gaaacgatca atagtacatt agctattgaa gactttttat
    94321 tagattcgtt agattttttg gcagaagtag tgattgtaag ggataaaaat cattctccac
    94381 aaccgattat tgctttagct cctgataaag aaatggattg gaatcgatgg tgggagcaag
    94441 tccatgaact tcctcgcttg aacgtcccta tcatccgtga ttttgatgca atcccgcgaa
    94501 cagctacaat gaaagtgcaa cggcttcaaa tcgagaaaga attaaaatca caaaagtcct
    94561 aactatttaa ggaaagaaag atccattttg gtctttcttt tctttttgtt tttttaaatt
    94621 ggatgagata aaaatatgcc aattggctgt ttttcttttt tgaaaatttc gttatactct
    94681 tattcatgga ggaaaatgtt atgaaaagaa tcttgacaat tggcggttct gatccatttg
    94741 ctggtggagg aatccaaaca gatttaaaaa cttttgagaa ttttggtcta ttcggactaa
    94801 gcgccttgac ttctataggg gcactagata ttaatgagcg atttatgctg gaatctatat
    94861 ctttggaatt gcttaaacga cagttagttt cgattgataa aatgacttca ttagatggta
    94921 tcaaaattgg gttattgaat tcaagtgaag caatcatggt tgttcgagac ttcataaaaa
    94981 caaaacaaaa tatcccaatc attttagacc ctgttttggc attcaaagaa actgaagctg
    95041 tgatcaatca attgtatatt tcgcagctca tcgaagaact ttttccactt gttacgatga
    95101 ttacgcctaa tttggcagaa gctgctttac tttctggtca agctcttccc tcttcgttaa
    95161 cagaaatgat ccatctcgct aaagaactca ctgcttcagg aacaaaaaac attgtgatca
    95221 aaggcggcac aaggatggct ggagatgaag ctgtggatct tctctattca attgatcatt
    95281 atgaggtatt ttctcataaa aaaatcgaca ctacaacagt aaacggggcc ggttgtgcct
    95341 tttcttctgc aataacagcg aatcttgtgt taggggcaga ttttaccaaa gcagtagcag
    95401 aaagtaaaaa atttgttgcc cattgtattt ctaatggcgt gctgatgaac gatcataccg
    95461 gaagtgtctg gtctggcggt attcaaaaag gaggaatgag aaatgaaaac taagcaatta
    95521 acgatctatg ctatgctgat tgcgttgaca gttgcccttt cattaacaat tttgatccct
    95581 gtaccggcta cgaatggatt tgtgacatta tgcgaggccg ggatctatac cgctgctttt
    95641 ctttatggtc caacgggtgg attagttgtt ggtgcttcca gtggtctatt gatcgatttg
    95701 atttcaggat atccgcagtg ggcagttttt tcattagtga tccacgggtt acaaggatat
    95761 gttgctggtt ctttcagcaa atcttctact cgtatgtggg gattagggct tttattagga
    95821 agcttagtta tgatcatcgg ctatggtatc gccggttgga ttttatatga ttggccggct
    95881 ggagtggctt ctataccagg aaacatcttc caaaatctga ttggaatggg acttgccttt
    95941 cctataactg caagtcttca acgtattcaa gcacggaagc aatattaatt gaaaaagaag
    96001 gttttagaaa atgaagttgt cagaaaaaga attaaaagac caattgactg aaatagttaa
    96061 tgacatattg gctgaggcac acttgaaaaa aggtgatctt tttgttttag gatgcagcac
    96121 aagcgaagtc gttggaggac atattggaaa aaattccagt gcggaagttg gtcaatggat
    96181 catccgtacg ttaaaagagt tgttggatcc taaagaaatt gcgctagctg tccaaggttg
    96241 tgaacatttg aaccgagcat tagtagtaga acgaacagtg gcagaagcaa aaaatttcga
    96301 aattgtctct gttgttcctg cactacatgc tggaggtgct tgttctgttg ctgcttttga
    96361 ccagttcaat gatcctgtcg aagtggaaca cgtagttgga caagccggta tcgatatcgg
    96421 tgacacatca atcggtatgc atatcaagca tgtccaagta cctgtcagac ctcgtttgaa
    96481 aacccttgga caagctcacg tgacagccct tcgctctcgt cctaaatata tcggtggtcc
    96541 tagagcgaat tattgaaatg agtaattgac agagcaatct gaaaaacttc ctcgcaaagt
    96601 ccaactaatg aactttaacg aggaagtttt acttgtttgt tttatataac cgagcttcat
    96661 aaggtgctag tatgccttta gcttcaaatt ctccgctaga ggaatttgac aagagtaatg
    96721 tccaggactg atctgcttct tttggaagtt catagtcagc aagtgattct gttaagttta
    96781 ccataataag atattgttca tcttctaaat atctttcata gacataaagt tgtggatgtt
    96841 catgaagata ttctttataa gaaccgtata tcagaccttt attggtttgt ctaatgcgga
    96901 tcatgttctt atagaattgc aggaccgact ctggttcata ttcttgttgt tctacattga
    96961 tcgttttggt atttggatta gtgatcagcc aaggttcatg tgtagaaaaa ccagcatatt
    97021 gttccgaagt ccattgcatg ggtgtcctgc tgttgtctct cgttgtttca cgaatgatat
    97081 ccaatgcttc ttctcttgtc ttaccttccg caagcagcct tttataaagt cttttcgaat
    97141 ctactgcatc aacttgatcg atcgattcaa agggcatatt cgtcattccg atttcctgtc
    97201 cttgataaat aaatggcgta ccttgcaaaa agaaatacat catagcgatt gcttttgcag
    97261 acatagccca aaaatcggga cgagtatctc caaacactga aacggcacga ggaacatcat
    97321 ggttttccat atataaagcg ttccatccga taccatccaa tgaagtttgc caagcgctta
    97381 atgcatgttt caatttaagg acatctaatt gcccttcttt ttcttgctgc cagatatgga
    97441 tatgatcaaa ttcgaatatc atatcgaaat aaccgtcttt ccctacccat tgtggtcctt
    97501 cttctgcagt gacaccactt gcttctccga cagtcatgat atcgtactct tcaaaaacat
    97561 gtttcaaatc ggtcaaatgt tcttcgattc ctgagacatt ttgaaagggt gagaaagggt
    97621 tctctgtggc ttttactgaa tattcatctt tttttacatg agaaatcgca tctaaacgaa
    97681 atccatctat ccctttatcc aaccaccatc ggatcatatt gaatagttct tctttcaatt
    97741 tctcgctttc ccaatttaag tctggttgtt cttttgcaaa aacatgcaga taatactgtc
    97801 cagtttcatg agaatactcc caagcagaac cgccgaaaat cgatgtccaa ttattcggtt
    97861 ctttgccatc taccccgtcg acccaaatgt aataatcgcg atacgggctt tctttagatt
    97921 ttttcgattc ttcaaaccat tgatgctgat ccgaactatg gttgatgaca agatccataa
    97981 tcactttgat ccctaattca tgtgcttctt ttagcaataa atcaaaatct tccatcgttc
    98041 cgaatctctc ttctatatct tgatagtcac ttacgtcata accattatcg acaaatggag
    98101 attgatagat cggattgatc cagataaagc cgataccaag atctttcaaa taattaagtt
    98161 tttcacggat cccattgatg tctccttgac catcgccatt gctatctttg aaactagcag
    98221 gatagatttg atagcctact gcttctttcc accatttttg ttcttgtatc atatcgtttc
    98281 actcctttaa cggtccctct tgattataac cgattctatt ttagaagtat ccttgttacc
    98341 ggtaactaga aacgaaaagc tcaacgaatc tgctaagtgc gttcgctgag ctttatttta
    98401 tcatagatag cgggcaatca gttgaatcat ttgcttggga aattcattca agtcagtaat
    98461 atctacaaaa cgactttcac cgtagatcga cttgatttct tctttgtctt ggccaatagc
    98521 tgcggagaca aataggattc cttgacgttc atattctttc aaaacagact ggatatcttc
    98581 ttttgctttt ttgcctgtat aatccggcaa cgcttttggc tgaccgtcac taatattaag
    98641 caacagtttg gttgttgcgg attgtctgtt taatttttct gctgctaaac gaagtgcagc
    98701 accatcacga ttgttttgcc ttggtttcat agtgactaat ttttcattta accattgaaa
    98761 tccgtcttca aattctttat aagaaaacaa agaagtcttt tctcttgacg aacggtctgc
    98821 tgtatctccg taaatcaaca atgggatatc gactttctta gaaaattcgg caatggcaat
    98881 cgctgctttt tttgccgctt cgatccgatc atcccgtatc attgacccag attcatcgat
    98941 ccgtacagct accgctaaag aaggttgctc atgtggcggg ttctttttgt caaaatttct
    99001 caaatcacca tatgctactt tgcttgcatt gaaccgctga ccttcgtatt ttcctttttg
    99061 gtacgtctct gtttgttcat tatccagtaa ctctaaaatt tgccggctaa gtgattctac
    99121 tattggcatt acttgcatgt gtagctcacg tgcttccggc gtttttcctt ggaaagctgg
    99181 tcggtgaacg atgagacctt ctttttcatg aatcgtttcc ttcatgattt gtcgcgcttc
    99241 tttattcagt tgcttcttga tttccttatc atgtgcttcc atgtcagaag cagacatttc
    99301 ttgagaatca tctcctgttt gttgttcttt ttgatttttt ccttcgatat cgctactgcg
    99361 ttctttttgc atttgaggag gaatctcgtt tttttctatt gtttcttctt gtttctcttc
    99421 tccctcattt ggttcttcag cagacaacga tttcttttca tctaagcgca gaatttcttc
    99481 agcattttca gtgatgtttc gttcatttgc ttcttcggcg ctaatggttc tttcttttgt
    99541 atgctcagat tgaggatgac cccatttttc ttttctcaat atgtcctgaa gttccagtag
    99601 caatccactg tcagacaaag cttgttcaag aattgccaat tcatctgaat cagtggataa
    99661 tttatacaaa acttttggat aaatcgactg gagtaaatct tgtccttgct tataggtatt
    99721 tgcccaataa aaataagaac gcattcctgc tacacctttg atggcgttag cttttgcagt
    99781 ttcatccaac aaacggataa tctctgccat ctttaagaga agttcttgtt tttggatcat
    99841 agttttcgcc atcgcacgtt ctgccatgac ttctaaggca ggaagatcca ttttttccgc
    99901 atgttgcatc cgatctctta aagattcatt taatgggcga tttccttggt aatttcgatt
    99961 tgtagtgatc acaatgacgc aatctggatg gcgacgaatg attcctgtcg gtaaattcaa
   100021 catcccattt ggttcgagcg ctgagttaag tgccacaaga acagaggcat ctcggatgac
   100081 agtcggctct tgaatctcta ataaatagcc tttttcaatc gctcgaacga tttcagaagg
   100141 gtaatagctg taatggattg catctggata atcagcttcg atcctttgaa ctagctctgc
   100201 taatttttct ttagcagaaa aaggatcaat gccgtaatgg ttctggatca gtgtcagtac
   100261 agcttcaagt gtatctgttt ggtaaatagc ttctagtaat tcttggtctt gttccttatt
   100321 agaatctact acaggtaata aggctccgaa aacatctgat ttgtccatat ctgcaaaaca
   100381 ggtcactttt gtatagggaa gttgcagatc agcagatagt gctttggcta attgtgtctt
   100441 ccccgacccg gcatctcctt ccaaaagtac gttactgatt ttcatttcag gatcttgcca
   100501 attccctttg atttcggagg cgatccgcca ttcttctgca cttgtccgat ggcttttcgg
   100561 tttcttccag atcatttgct tttctagccg tgaaaaggtt cggttttctg ttagtgagaa
   100621 attctccatg cttctcttcc ccttttgatc atctctgtct attaatatcc tagctcatct
   100681 aaaagacgtt ttaaaatacg gaatcgttcc ccaatcagtt ctccgtcttt ttgtagtgta
   100741 tcgtaataca aaatgatgtc ttcatcgatc tttggatttt tggtatctac ttctaccgtc
   100801 attcggttcc cttgtaatcc aatgatgtct acgactggat tttctggatc ataaaatttt
   100861 tcataacgat aagcatcttg gaagtataaa cttccttgcg cagctaagat cgctaaatct
   100921 aaaatacgtg tgattggtaa ttcttccgac tgtcgtgacc atttttctcc agtatgtcgc
   100981 cataccttgc ctgagatatc gacttttcca cgatcattcc actgagcaag gcccagtgaa
   101041 agcccttttg catccatatt taatgcattt cttccatcaa ctttatcata gtcttctaca
   101101 acgattactg gtttatgttt taaatttgtt ggtatttcca ttattttaac tcctcatcct
   101161 tttattttta gtaaataagt aaattagtag tttactcatt tactaaacta tatgcgacat
   101221 tatagaggat tgaagtgagg attgcaaaga aaaagagctt cattgataga aataaaaaca
   101281 ccatctcgtt cttttacacg ctcattagta acatgtagaa agtccaagat gatgttggaa
   101341 t
//
LOCUS       DACEDD010000002        91967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEDD010000002 DACEDD010000000
VERSION     DACEDD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.002"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..933)
                     /locus_tag="IWO45_000092"
     CDS             complement(<1..933)
                     /locus_tag="IWO45_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331604.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEE"
     gene            complement(1188..1697)
                     /gene="mreD"
                     /locus_tag="IWO45_000093"
     CDS             complement(1188..1697)
                     /gene="mreD"
                     /locus_tag="IWO45_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0331605.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1701..2555)
                     /gene="mreC"
                     /locus_tag="IWO45_000094"
     CDS             complement(1701..2555)
                     /gene="mreC"
                     /locus_tag="IWO45_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0331606.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2710..3348)
                     /locus_tag="IWO45_000095"
     CDS             complement(2710..3348)
                     /locus_tag="IWO45_000095"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0331607.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3349..3999)
                     /locus_tag="IWO45_000096"
     CDS             complement(3349..3999)
                     /locus_tag="IWO45_000096"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0331608.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4012..4911)
                     /gene="rsgA"
                     /locus_tag="IWO45_000097"
     CDS             complement(4012..4911)
                     /gene="rsgA"
                     /locus_tag="IWO45_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0331609.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5076..7145)
                     /gene="pknB"
                     /locus_tag="IWO45_000098"
     CDS             complement(5076..7145)
                     /gene="pknB"
                     /locus_tag="IWO45_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0331610.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7142..7882)
                     /locus_tag="IWO45_000099"
     CDS             complement(7142..7882)
                     /locus_tag="IWO45_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0331611.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7895..9253)
                     /gene="rsmB"
                     /locus_tag="IWO45_000100"
     CDS             complement(7895..9253)
                     /gene="rsmB"
                     /locus_tag="IWO45_000100"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0331612.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9253..10191)
                     /locus_tag="IWO45_000101"
     CDS             complement(9253..10191)
                     /locus_tag="IWO45_000101"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0331613.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10184..10675)
                     /gene="def"
                     /locus_tag="IWO45_000102"
     CDS             complement(10184..10675)
                     /gene="def"
                     /locus_tag="IWO45_000102"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0331614.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10690..13098)
                     /gene="priA"
                     /locus_tag="IWO45_000103"
     CDS             complement(10690..13098)
                     /gene="priA"
                     /locus_tag="IWO45_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0331615.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13351..13656)
                     /locus_tag="IWO45_000104"
     CDS             complement(13351..13656)
                     /locus_tag="IWO45_000104"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0331616.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13661..14275)
                     /gene="gmk"
                     /locus_tag="IWO45_000105"
     CDS             complement(13661..14275)
                     /gene="gmk"
                     /locus_tag="IWO45_000105"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0331617.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14762..15046)
                     /locus_tag="IWO45_000106"
     CDS             complement(14762..15046)
                     /locus_tag="IWO45_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331618.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15064..15954)
                     /locus_tag="IWO45_000107"
     CDS             complement(15064..15954)
                     /locus_tag="IWO45_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0331619.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(15974..16192)
                     /locus_tag="IWO45_000108"
     CDS             complement(15974..16192)
                     /locus_tag="IWO45_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331620.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16162..17862)
                     /locus_tag="IWO45_000109"
     CDS             complement(16162..17862)
                     /locus_tag="IWO45_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0331621.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18129..18902
                     /locus_tag="IWO45_000110"
     CDS             18129..18902
                     /locus_tag="IWO45_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331622.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(18983..19552)
                     /gene="thiT"
                     /locus_tag="IWO45_000111"
     CDS             complement(18983..19552)
                     /gene="thiT"
                     /locus_tag="IWO45_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0331623.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19618..19720)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19726..20697)
                     /locus_tag="IWO45_000112"
     CDS             complement(19726..20697)
                     /locus_tag="IWO45_000112"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0331624.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20791..22164)
                     /gene="glmU"
                     /locus_tag="IWO45_000113"
     CDS             complement(20791..22164)
                     /gene="glmU"
                     /locus_tag="IWO45_000113"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0331625.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22667..23485)
                     /gene="purR"
                     /locus_tag="IWO45_000114"
     CDS             complement(22667..23485)
                     /gene="purR"
                     /locus_tag="IWO45_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0331626.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23652..24476)
                     /locus_tag="IWO45_000115"
     CDS             complement(23652..24476)
                     /locus_tag="IWO45_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0331627.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24449..25129)
                     /locus_tag="IWO45_000116"
     CDS             complement(24449..25129)
                     /locus_tag="IWO45_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0331628.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25152..26090)
                     /locus_tag="IWO45_000117"
     CDS             complement(25152..26090)
                     /locus_tag="IWO45_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0331629.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26335..27186)
                     /locus_tag="IWO45_000118"
     CDS             complement(26335..27186)
                     /locus_tag="IWO45_000118"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0331630.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27340..29913)
                     /gene="mprF"
                     /locus_tag="IWO45_000119"
     CDS             complement(27340..29913)
                     /gene="mprF"
                     /locus_tag="IWO45_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0331631.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30054..30860
                     /gene="proC"
                     /locus_tag="IWO45_000120"
     CDS             30054..30860
                     /gene="proC"
                     /locus_tag="IWO45_000120"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0331632.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30939..32495)
                     /gene="rny"
                     /locus_tag="IWO45_000121"
     CDS             complement(30939..32495)
                     /gene="rny"
                     /locus_tag="IWO45_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0331633.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32906..33955)
                     /gene="recA"
                     /locus_tag="IWO45_000122"
     CDS             complement(32906..33955)
                     /gene="recA"
                     /locus_tag="IWO45_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0331634.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34064..35305)
                     /locus_tag="IWO45_000123"
     CDS             complement(34064..35305)
                     /locus_tag="IWO45_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0331635.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35545..36123)
                     /gene="pgsA"
                     /locus_tag="IWO45_000124"
     CDS             complement(35545..36123)
                     /gene="pgsA"
                     /locus_tag="IWO45_000124"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0331636.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36332..37216)
                     /locus_tag="IWO45_000125"
     CDS             complement(36332..37216)
                     /locus_tag="IWO45_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0331637.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(37994..39280)
                     /locus_tag="IWO45_000126"
     CDS             complement(37994..39280)
                     /locus_tag="IWO45_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0331638.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39273..40541)
                     /locus_tag="IWO45_000127"
     CDS             complement(39273..40541)
                     /locus_tag="IWO45_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0331639.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40793..41254
                     /gene="mscL"
                     /locus_tag="IWO45_000128"
     CDS             40793..41254
                     /gene="mscL"
                     /locus_tag="IWO45_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0331640.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41313..41759)
                     /locus_tag="IWO45_000129"
     CDS             complement(41313..41759)
                     /locus_tag="IWO45_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0331641.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41852..42097)
                     /locus_tag="IWO45_000130"
     CDS             complement(41852..42097)
                     /locus_tag="IWO45_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331642.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42213..42791)
                     /locus_tag="IWO45_000131"
     CDS             complement(42213..42791)
                     /locus_tag="IWO45_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0331643.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42868..43398)
                     /locus_tag="IWO45_000132"
     CDS             complement(42868..43398)
                     /locus_tag="IWO45_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0331644.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43402..44262)
                     /gene="rlmB"
                     /locus_tag="IWO45_000133"
     CDS             complement(43402..44262)
                     /gene="rlmB"
                     /locus_tag="IWO45_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0331645.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44255..44650)
                     /locus_tag="IWO45_000134"
     CDS             complement(44255..44650)
                     /locus_tag="IWO45_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0331646.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44647..46059)
                     /locus_tag="IWO45_000135"
     CDS             complement(44647..46059)
                     /locus_tag="IWO45_000135"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0331647.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46056..46592)
                     /gene="cysE"
                     /locus_tag="IWO45_000136"
     CDS             complement(46056..46592)
                     /gene="cysE"
                     /locus_tag="IWO45_000136"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0331648.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(46914..48374)
                     /locus_tag="IWO45_000137"
     CDS             complement(46914..48374)
                     /locus_tag="IWO45_000137"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0331649.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPKMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48544..49701)
                     /locus_tag="IWO45_000138"
     CDS             complement(48544..49701)
                     /locus_tag="IWO45_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0331650.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49735..51105)
                     /gene="radA"
                     /locus_tag="IWO45_000139"
     CDS             complement(49735..51105)
                     /gene="radA"
                     /locus_tag="IWO45_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0331651.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51168..51695)
                     /locus_tag="IWO45_000140"
     CDS             complement(51168..51695)
                     /locus_tag="IWO45_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0331652.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51925..52731
                     /gene="proB"
                     /locus_tag="IWO45_000141"
     CDS             51925..52731
                     /gene="proB"
                     /locus_tag="IWO45_000141"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0331653.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52728..53975
                     /locus_tag="IWO45_000142"
     CDS             52728..53975
                     /locus_tag="IWO45_000142"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0331654.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54051..54395
                     /locus_tag="IWO45_000143"
     CDS             54051..54395
                     /locus_tag="IWO45_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331655.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54512..54838)
                     /locus_tag="IWO45_000144"
     CDS             complement(54512..54838)
                     /locus_tag="IWO45_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331656.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55453..56655)
                     /locus_tag="IWO45_000145"
     CDS             complement(55453..56655)
                     /locus_tag="IWO45_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0331657.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56772..57287
                     /locus_tag="IWO45_000146"
     CDS             56772..57287
                     /locus_tag="IWO45_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0331658.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57486..57932)
                     /locus_tag="IWO45_000147"
     CDS             complement(57486..57932)
                     /locus_tag="IWO45_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0331659.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(57979..59457)
                     /gene="msr"
                     /locus_tag="IWO45_000148"
     CDS             complement(57979..59457)
                     /gene="msr"
                     /locus_tag="IWO45_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0331660.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59811..60440)
                     /locus_tag="IWO45_000149"
     CDS             complement(59811..60440)
                     /locus_tag="IWO45_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0331661.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60424..60888)
                     /locus_tag="IWO45_000150"
     CDS             complement(60424..60888)
                     /locus_tag="IWO45_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0331662.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61375..62643)
                     /locus_tag="IWO45_000151"
     CDS             complement(61375..62643)
                     /locus_tag="IWO45_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0331663.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(62874..63611)
                     /locus_tag="IWO45_000152"
     CDS             complement(62874..63611)
                     /locus_tag="IWO45_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0331664.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(63678..64246)
                     /locus_tag="IWO45_000153"
                     /pseudo
     CDS             complement(63678..64246)
                     /locus_tag="IWO45_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            64374..65246
                     /locus_tag="IWO45_000154"
     CDS             64374..65246
                     /locus_tag="IWO45_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0331665.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            65588..66460
                     /locus_tag="IWO45_000155"
     CDS             65588..66460
                     /locus_tag="IWO45_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0331666.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            66457..68415
                     /locus_tag="IWO45_000156"
     CDS             66457..68415
                     /locus_tag="IWO45_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0331667.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(68747..69598)
                     /locus_tag="IWO45_000157"
     CDS             complement(68747..69598)
                     /locus_tag="IWO45_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0331668.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            69721..70188
                     /locus_tag="IWO45_000158"
     CDS             69721..70188
                     /locus_tag="IWO45_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0331669.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(70451..70933)
                     /locus_tag="IWO45_000159"
     CDS             complement(70451..70933)
                     /locus_tag="IWO45_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0331670.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71033..71896)
                     /locus_tag="IWO45_000160"
     CDS             complement(71033..71896)
                     /locus_tag="IWO45_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0331671.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            72494..73369
                     /locus_tag="IWO45_000161"
     CDS             72494..73369
                     /locus_tag="IWO45_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331672.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(73514..73666)
                     /locus_tag="IWO45_000162"
                     /pseudo
     CDS             complement(73514..73666)
                     /locus_tag="IWO45_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(73934..75265)
                     /locus_tag="IWO45_000163"
     CDS             complement(73934..75265)
                     /locus_tag="IWO45_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0331673.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(75414..76127)
                     /locus_tag="IWO45_000164"
     CDS             complement(75414..76127)
                     /locus_tag="IWO45_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0331674.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            76302..77726
                     /locus_tag="IWO45_000165"
     CDS             76302..77726
                     /locus_tag="IWO45_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0331675.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            78025..78732
                     /locus_tag="IWO45_000166"
     CDS             78025..78732
                     /locus_tag="IWO45_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0331676.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            78719..79042
                     /locus_tag="IWO45_000167"
     CDS             78719..79042
                     /locus_tag="IWO45_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0331677.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(79131..80168)
                     /locus_tag="IWO45_000168"
     CDS             complement(79131..80168)
                     /locus_tag="IWO45_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0331678.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(80489..81472)
                     /locus_tag="IWO45_000169"
     CDS             complement(80489..81472)
                     /locus_tag="IWO45_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0331679.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(81887..82657)
                     /locus_tag="IWO45_000170"
     CDS             complement(81887..82657)
                     /locus_tag="IWO45_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0331680.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(82650..83585)
                     /locus_tag="IWO45_000171"
     CDS             complement(82650..83585)
                     /locus_tag="IWO45_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0331681.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(83901..85550)
                     /locus_tag="IWO45_000172"
     CDS             complement(83901..85550)
                     /locus_tag="IWO45_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0331682.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(85667..88270)
                     /locus_tag="IWO45_000173"
     CDS             complement(85667..88270)
                     /locus_tag="IWO45_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0331683.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(88282..89331)
                     /locus_tag="IWO45_000174"
     CDS             complement(88282..89331)
                     /locus_tag="IWO45_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ0331684.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(89357..89671)
                     /locus_tag="IWO45_000175"
     CDS             complement(89357..89671)
                     /locus_tag="IWO45_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0331685.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(89687..90133)
                     /locus_tag="IWO45_000176"
     CDS             complement(89687..90133)
                     /locus_tag="IWO45_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0331686.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(90134..>91967)
                     /locus_tag="IWO45_000177"
     CDS             complement(90134..>91967)
                     /locus_tag="IWO45_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0331687.1"
                     /translation="LYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETL
                     RNLLSSHKINIADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRK
                     RLNKHGMSLVSDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIG
                     EANNFLNKDILKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSN
                     NSEEEISKNTLILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLD
                     MGVELQELVRIKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRS
                     FMISVDLLEEIEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKL
                     LEQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGAD
                     VRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGI
                     LEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALN
                     EKVKPELNKIYETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT        26276 a        19425 c        16141 g        30125 t
ORIGIN      
        1 ttcttctgtt gaagattcag gtgttgttga ttcttctgtt gctgaagatt gtgtagctga
       61 tgatgattct tcagtcattg aagattgtgt agctgatgat tcttctgttg ctgagctttg
      121 tgcagtagat gaagcttgag tagcttgtgc ttcagcctct gcttgttcgc gtgcttcttt
      181 ttcagctttt tcttgtgcag cttgttgacg agcttgttct gctagacgtt gttgttcacg
      241 aatacgagct tgctctgctt cagcttctgc tttttgacgg ttcaagtcag ctttcttgtc
      301 ttcagcagtt gcttgttcag cagctaatga agttttcagt acgttcaaat cagcttgttt
      361 agataaaaga tcgccttttt gagattccaa agcagcttgg ttttcagcta attcttttag
      421 tttagcatcg ttttcagctt ttttgtcttc tacagctttt ttatcttgtt tttgttgttc
      481 catcaagtcg ttgtttgctt ttaccatcgt tgtcattgct tgaacgcgtc caatagcatc
      541 tgctaatgaa tcagcattca atacagcatc gatgtagttt gagcttgtgt tgcttacttg
      601 tgcttcgcgt gcttgttttt ggattgtatc ttcacgtttt tcaatacgtt cttgtaaatc
      661 tgcaatgtct tttactaatt gtgcagattc ttgacgcaat gtatcttgtt ttgctaatag
      721 gtcttgtgct tgtgtgttga tttcagaaac ttggctttct aaggcatcga tttgtgattg
      781 tgcatctgct tgttgatttt tcaagtctgc aatcttttga tcttgctgtt gtatctgaga
      841 atcaaaatcg tccgccgcgg cagcgatcgg tgatgctacg gcagtcaagg tcattgaaca
      901 taccattact gctgatatta aactcttttt cactcttcat tcctccgact ggcttaattt
      961 aataaatatc taaatatatt taagtctttc ttaacgatat aaaaattgta ccatagcagt
     1021 gtgtcaacga tataatagtt atgttacaaa aatgtttcaa aacaactttt aaaaatgaca
     1081 aaactgttcg tttcagaaaa tgatcatgaa aaagccgcgt taagtctagc atttcttaag
     1141 ataacaacga ttctttttcg ctttcgacct aaaaataaaa aaagaaatta ttcatcactg
     1201 aataatttct taaagggaaa aatgaataat acaaatataa tcatgttcaa cagcagtgta
     1261 ggtcctaaaa accttgtaat aaaatacgtg ttatttacta ctgctaattt aaaaatcaac
     1321 tggaccacca tcgtaaacaa ttcataaccc gtgacaaaga tgatcatact gaaaaattca
     1381 gtaaagatgt tgacatggat aacgtctttc attacataca tcaaccatac aattaaaggg
     1441 agagctactg catagatacc gataacgcca atatagtaag cgtcatagat agccccgaga
     1501 acaatcgtgg taatcagtaa ataacgtttt tcaaacgcca tactgcaaca taataatgcc
     1561 aatatcagaa agtgggcatt tgacatatag gtgcctttcg accactcccc tagcattctt
     1621 gttaaatgac catctatcaa catcaaaaag aacaaaacga ttggcagata gtacttcatt
     1681 gtttctttct tcaacatgga ctaatcctcc gctagccgtt ggacaatcgt cacaactgac
     1741 acgtcataca tttctgcata aggtttcaca taaacttccc gatccagccc ataactatct
     1801 ggtttcgttt taataaccgt accgatcggc aagttagctg gagaattccc tccaagaccg
     1861 gatgtctgga caacatcccc ttctttgata tccgtatcac cagttaattg ggtcaccact
     1921 aaggcatgga gcttttcatc atagttttta agcaaaccaa acgcttcgcc attagccgaa
     1981 gatacccgta ctggaaaatg attggagctt tcattagagg atgacagtaa ttcgatttta
     2041 gacgaagccg tattgacctc gatcactcgg ccaatcaatc ctttttgcga catgactgcc
     2101 atatttgctt cgataccgtc tttagaaccc ttgtctacga tcagcatgtc ctgccaactg
     2161 tcaggcgaac gagtaatgac atttgccgtt actttttcgt aactagtcag cgtctgattc
     2221 aactcaagtt cttttttcaa cgcttcgatt tctctctcgt aattcttgtt ttgctgtgaa
     2281 acgtcatcat agctatcgat tttttctttt agccgctcat tttcggcata agttgaaaaa
     2341 agattatgga cagaatctac tccatttcgt acccaccgta ctggggcaga gattgtttta
     2401 tcaaccattg caacactgtc attaacgatg gattggaaga aattgggctt tccttcattc
     2461 gctcgctgcg cagcggtcac acttagtatc gtcaccacga tgataacaat aaccaaggta
     2521 ataataatat ttttattagg attgaacttc ttcacaaaga cacctcgaat taattagact
     2581 cttgtgttca attttatcac taaatgccgt gaaacaaaag gtaagttgct cctcttaagc
     2641 ttttttttag caaataatag attctgtcat ttttagcctg aaagacaaat ttttttattt
     2701 gtttatggat caatctttgc tttgaataac tgcaatcata ccatctgaaa aagaaaattc
     2761 tgcggtatct gaaagaaatt cgttacttgc ataagatgtc ggatattcta cttctttatt
     2821 ttccaataca tacttgctgc cttttaatgt aagattcgag acgggagtca agcagcaata
     2881 agctaaatac ttcatatctg gtatctgtct tactatatgc tctccaggca aataataaga
     2941 caaataattt tgacaatctc tgataataag ctgatgggca aagggtttga agcgaggttc
     3001 catacctaac cagagattgg ctaaaagatg atccaatctt ccgcccgtag ctccaatcaa
     3061 agtgacttcc gcttgcgggt aacgttcaaa tgtttttaag atacctaact gtgtatctgt
     3121 atcgtctttt tctgcagggg aacgggtata ctctttggct tttgcaaata cttccttctg
     3181 ctcttcgttc gacaatgaat caaaatcgcc cactgccaga tctactgggt agcctgcttg
     3241 aagcaggtac agcgcaccac ggtctatccc gacataggca tcgtatttgg acagcagaga
     3301 ctgcggccag ttttgcggat tacctccagc agctaataaa attttcattt agtctaatgc
     3361 ctcttttaat cgttcgattt gtccgactgg attttcggct ccatagatat atgatcctgc
     3421 aacgaagaca tccgcccctg catctttaca gatttttgct gtttctggaa cgattccgcc
     3481 atctacttcg attgcataag tccaattgtt ctctctacgt agatcagcga gttccgtaat
     3541 tttttctact gtttcttcaa taaagctttg tccgccgaac ccaggattga cagtcatgac
     3601 tagtacttga tcagccaaag gcaaaacatg tttgatcatg gagactggtg ttcctggatt
     3661 gataactact ccagctttaa ctcctaagct ttttgtcatt tgaagtgccc gatgaatatg
     3721 aggagttgct tcgacgtgga cagtaatgat atctgctcca gcttttgcaa atgctggaat
     3781 gtagttttct ggatcaacga tcatcagatg gacgtctaac ggtaatttag taacaggacg
     3841 gattgcctga acaatatttg gccctaatgt gatatttggg acaaattggc catccattac
     3901 atctacgtgg atgtaatctg cgcctccttt ttccactaac tcaatgtctc tttgtaaatt
     3961 ggcaaaatct gcactcaaaa tggatggtgc tatcttcatt cttgtttcct cctattttcg
     4021 tttaccttct ttttttgcat acattggttt tcttttttcg atttcttgta agaattgcaa
     4081 ataattgtcg taacgtgtct gtgcgatacc acctgtttct actctatgtt ttacttcgca
     4141 gccaggttct ttagcatgca tgcactctct aaatttgcaa tggacagaag cttctacaaa
     4201 ttcaggaaat tgtctcggca gttctcctgc ttcaattgtc aaaaagtcaa tcgagctaaa
     4261 tcctggtgta tctgctacta acccgccata gataggaatc agttccacat gacgtgttgt
     4321 atgccgtcct cttccaagtg catcagaaat ctctcctgtt gctaaattca gttccggtga
     4381 tatttgattc agtagcgtcg attttcctgc tccagactga cccataaaga ccgatagccg
     4441 ttctgggaag taacggatca gttctgtcgt atcacccgta tgattgggta caatcaccgg
     4501 atagcctatt ttagtatagg tttgcttgat ctcttcgatt ttttctgggt cgtctgacaa
     4561 gtcggtcttt gtcaaaaaaa taatcggttc gatcgactcg tattccaagg taactagaaa
     4621 gcggtcaagt agattataag aaaatgcagg ctctacgata cttgtgacaa tgattccaag
     4681 atcaacattt gtgactggtg ggcgaactaa ttgatttttt ctcggcaaga tttccagaag
     4741 atagccgtct gtttgatttt cactctcaaa gatcacctga tctccgacaa gcggcgtgat
     4801 tttccgattc cggaaatttc ctcttcctct tgtttgatag gtctctccgt ttgcgtatac
     4861 atagtaaaaa ccgctgattg cttttctgat ttgtcctttg ataagtgcca cctccgtttt
     4921 tgtttatttt accatacgaa gaggaaagta ggtgttattc catgcaaact tctcaaagta
     4981 tatggcttga aaattgatca aaagagatag aaacacaaaa aagagaatgc aacaaatcaa
     5041 tgtcgcattc ccttctgata tattttattc agcacttatt ttgaactttc tgaggtgcta
     5101 gctgatgaac ttgtggagct ggaagattct tcttttgaag agttgcttgt gctactggag
     5161 ctacttgagc tgctagaact gtctgaagag tctttttcag aactagaaga cgctggatct
     5221 ggtcctttag agtaaattac gttcacaacc attcctttgg taattgtcgt tcctgcccct
     5281 ggtactgtac gaatcacttt atctttatcc accgtgtttg aatattcgga agtctcatgc
     5341 ccgatatatt ctgcaccgac actagctaaa taactttgtg cttcgctttt tgtatagccg
     5401 gagatatcat tcaatttagg ctctgttcct tggcttacga caaaattgat gtaagtttct
     5461 tctgctacaa cttcacccga ggctggatcc tgactgatga tcgaacctga tggtacagtg
     5521 tcgctatagt cataagtagc actaatatta ttgatattga atccttgtgt aacaagccaa
     5581 ttacgaacag tttcgtagtt ttctcctaca tgactactca aagtcactgt ttctggtcct
     5641 ttgctgactg tgaaagtaat cgtgtctgtt ttaggatcga cttctgtatc ttctgatgga
     5701 gattgcgaaa tgatcattcc gctttctacc tctgaactga attcctcttc tttttgaatc
     5761 aagtcagcag aaaaccctaa ttctttcaaa cttgcttcgg catcttcata acttttacca
     5821 ctgtaatcat ctaatttgat ttttttcgtg ccgctactaa tatacagagt gacttcgcgt
     5881 ttttgcttga ctgtcgttcc cgctgccggg tctgtcttca ctacattacc ttcttcgatt
     5941 ttgtcatctg caatttcttc tgtttttgtt gctacttcta atttcgcttt tgcgagtgtt
     6001 tctctagcag aagcttcaga aagatcagaa acatcaggaa caacaacttc tcctcgcccg
     6061 ccagaagcaa agaatacagc tgttccgatt cctaaaacag caagaatagc taataggatc
     6121 agccaccatt ttttctgtcc tttcttttta ggatcaggca gaacttcttc ctcctctggt
     6181 aaggtttcgt tttctggctt gtcttttggc agaggcatag atttgaatga atcaggcatt
     6241 ggtgcatctt ccgtaagggg tgtgataact tttgtttcat tatccatcac atgaggctgc
     6301 catttttctt cgtttgcacg agctggtgat aatactgtag aaagatcatc agacatttcc
     6361 tcggctgact tataacggtc tgcaggttct ttagccgttg cccgtaaaat cacgttttcc
     6421 agtgcttgtg gcacatttgg atcaagtgct tttatagatg gaagatcatc ttgaaaatgt
     6481 tttaaagcaa ttgttacagc agattcacca tcaaacggta cgctacccgt caacatctca
     6541 taaaggatga tccctaaagc ataaatatct gactgcttcg ttgccatact tcctctggcc
     6601 tgctcagggg acagataatg aacagatcct agcatcgtat tcgtctgcgt gatcgatgtt
     6661 tcagataaag cgatagcaat cccgaaatcc gtgattttta caacaccttc attatcgatc
     6721 agtacgtttt ggggtttcaa atctcggtgg atgatttgat ggttatgagc taaagaaata
     6781 gcagaaagga tctgctgcat aatatttact gctgtttcat aaggaacagg ataatgtgtt
     6841 tggatatatc gtttcaagtc cattcctttg acgtattcca tcaccagata ctgcatgttg
     6901 tcttcttcac caacatcgta aacactaaca atgtttggat gaacgagttc tgttgctgct
     6961 aaagcttctc tctgaaatcg tcgaatcgca gtctggtcat tttgaaagtc aaatcgtaag
     7021 actttaactg ctacatcacg atccaagatt aaatcgtgcg ccaaaaatac atttgccata
     7081 ccaccgctac cgatatttcc agtgatttga taacggccat ttaatttttt gcccaactcg
     7141 atcatacact gtcctcctta tattcgataa gaagaacggt gatattatcc gctccgcctg
     7201 cttcattggc acgatcaatc agctcagtta ctttatgaga caatgggcct tcttgattga
     7261 tgatcgttcc tatttcttct tcagacagca tgttcgtcaa gccatctgaa cataacagca
     7321 gtcgatcttt tagttgccaa agatgagtag aaacatctac ttcgactgtt ccaggcatgc
     7381 cgacagaacg tgttaaaata tttttccttg gatggttcac tgccatttct tcgctgattt
     7441 ctcctgattt gactagttca ttgactaaag aatgatcctc cgtcaattgc atgattttat
     7501 cttcacgaat caaataagct ctgctatccc caacattggc aatcgtgaac cagtcatctg
     7561 acagagcggc cgctacgatt gttgttccca taccgttata ttccggctgt tcttgtccac
     7621 gctgataaat cagcatattt tcttcttgaa tcgattgaat gaaccattgg gcgatttttt
     7681 catcgtcaac agcttcttgt ttttcccaag cttcgcctag attcgttacc gccatctgac
     7741 tggcaatgtc tccggcacga tgaccaccca tcccgtctgc taatacagca agcttgagac
     7801 ctgaacggct ggtaaataca ctggcatagt cttgatttgt gttacgtcgt cttcctacat
     7861 ctgattgata ttcaatctgc attttttcac ctcattactt tttgcgcaaa caacaaatga
     7921 aaaatccatc tgtgtaaaac tgatgcggat acaaagtcaa cattttctct tcgattgatg
     7981 attgtagcat aggattggtc aaaacatcaa tcctctcaaa atcagggtgt ctttccaaga
     8041 aagcagaaac aacgtcttgg ttttcttctt ttaagatcgt acaagtgcta taagtcagaa
     8101 taccggaaga ctttaaagtt ggtgcgcagc tttctaaaat ttccaattgg atttttggta
     8161 aattcgctaa ttcatccggc tgttttttat aacgaatatc tggttttcgc cgaagcagcc
     8221 caagacctga acaaggcgcg tctactaaaa tccgatcaaa ctgctctgct tcgaactgtt
     8281 tactgacttc tctagcatcc atcttttgag cgtagacagc agaagagaca cctagacgtg
     8341 ctgcattatc ttcaatgagt ttgactttgt gttcgtgtac atctaaagcg accacttctc
     8401 caccgcatga cggatctaaa aaagaagcaa tatgcgtcgt cttacctccg ggtgctgcac
     8461 aggcatctaa gacatgatgt tcaggctcga tttgcataga cggcgctaca agcatcgaac
     8521 tttcatcttg gatcgtcatg acgccatcgt ggaacaatga gctagaagct aaatgccctt
     8581 ttttcgctac tataccgtat ggactgactt ggcttttttc agctgtgatc tcttcttctt
     8641 gcaaaacagt gatggcctga tctctggaaa gtcggcgtgt atctactcgg gcactagcat
     8701 ggctaggctg atatagcgat aaaccgagct tttcagtttc ttcgtatccg atttgatcca
     8761 ccagtcgttt cgtcatccat agaggcaggc tgattttgac agaaagtctt tcgataggat
     8821 cagcaatgcc ttgcagatct ggtgctcctt gacgctgata attcctcaag acaccattta
     8881 caaatttccc tgtcccagga tttcctttta cttttgcgat ttcgaccgcc tcgtttaaaa
     8941 tagcatgtgc agggacttta tctaaaaatt ctaattggta tacagaaaga taaagaagca
     9001 tcttgaccca gtcatccact ttttttgctt tagcgatcaa tggtgcaacg taaaattcca
     9061 atagtcgctg atggctaatc gtaccataga tcaattctgt aaataaaccc acatcttttt
     9121 gatttagctc gctttgtttg atcatctcat tcaacaaaag attcgagtag gcgccaccct
     9181 tgttcactcg ctctaatgtc agtaatgcca tatatcgtac tgattttttc atttttttag
     9241 ggattttttt cattatttca cctgctgacc aatctgaact tgctgaccgc ttccgttaag
     9301 gaaatcatgt acggcctgtt ttcctttacc agctggctgt aattcattga tggctaagac
     9361 ggtctgttta ccgcaagcaa tccatagatt ttttttgtct ttccggatga tcgtaccagg
     9421 tgctttgtct gtagtttctg ccaatacttc aaccgataat aatttccagc gtatgccctc
     9481 ataggtcgtg aatgcaattg gccaaggacg cattccgcgt acttggttgt caatttcttc
     9541 tgcagtttta tgccaatcga ttgcttcttg ttcccgtgtg atgtttggcg aaaaagttgc
     9601 ttctgactcg tcttgaggta ctggtttttg tccatccaaa atattgggca atgtttctag
     9661 caatagcttt cttcctaaaa ggctaagctt ttcaaacatc gtccctacat catcttgttt
     9721 agtgattggt atgctttctt gcgcatagat tccgcctgca tccatttttt tgatcatttc
     9781 catgatcgtt acaccagttt ctttctcccc attcatgatg gcataatgaa caggcgcacc
     9841 tccccgatat ttaggaagta aagaagcatg gacattgatt gcccctaatt ttggtacttg
     9901 caataacttt tcaggcaaga attggccaaa tgccgcagtg acgatcacat caggcaccaa
     9961 ttcaatgatt tcttccattt ctggcgaacc ggagattttc tctggctgca gcacacggat
    10021 accgtgcttc agagccgctt tttttaccgg tgtcggcgta attactcgtt tccgcccaac
    10081 tggacggtcg ggctgtgtta ccactgcctg gatctcgtaa cctgattcta tcagcccttc
    10141 aagaatcggc acagaaaaag caggtgtgcc cataaatact agcttagtca tcaagatgtt
    10201 cctccatata ttcttcaagt tcttcttccg ggatctgctg tatcattttc tcaatgaaca
    10261 agataccgtc taagtgatcg atttcatgct ggaaggcacg ggctaaatag ccaaaagccg
    10321 tcacttctat ctcttctccg tctcgatcat aataacgcac ggtcacttca tctgctcgct
    10381 tgaccgttcc atagacatgc ggaatactga gacaaccttc tacgtcaagg ctttcacctt
    10441 ttgcttcgat gatttctggg ttaatcagtt caaatttctc gccttcatct acttcgatca
    10501 cagcaattct cttgttttgt cctacttgag gagcagcgat ccctattccg tcattcgcaa
    10561 tcattgtctc atataaattg tctaataaag cgattgtctc gtccgtgatt acatcgattg
    10621 gctgagcagt tctttttaat ttatcattag ggtggatcaa tataggatag cgcataattt
    10681 ggccctttct taaataaagt ttaatggttc gttgtcaatt gccacaaaaa gaccttgtcg
    10741 ctgttctttc tggcttccat tcaaaatttc atccaaaaca tgaggaagat tcggttcatg
    10801 tttatatttg atgattagtt gataataata tcggtttttc actctcaaaa tcatgctggg
    10861 cgttggccct aatagcaagc tttgagggct taacacttct tttagctgat tggcaatctg
    10921 aaagattttc tttgccgcta cttgttcttc aggatggctg gcggttattt tcacagtaaa
    10981 gtaataagga ggatagcctc cacgatgccg tacatgcatt tcctgctgat aaaactgctc
    11041 ataattttgt tttttagcca attctatggc gtaatgttga ggattgaatg tttggatcac
    11101 gacttcgcct gctttttccg ctcgccctgc tcgtcctgaa acttgggtaa gcaattggaa
    11161 tgtatgctca ctagaacgaa aatccggtaa attcagggag gtatcagcat tcaacacccc
    11221 aactaatgtc acttctggaa agtccaaacc tttagcaatc atttgtgtcc ctaataatat
    11281 atctgcttct tttgcaccga atttttgtaa aatctgttcg tgtgcccctt ttcttcgagt
    11341 ggtatctaca tccatacgca aaatcctagc ttgcggataa agttctcgga gttcctcttc
    11401 tactttttga gtacctgttc catagtagcg gattttattg ccgccacaat taggacagcg
    11461 attgggaata ggttcttcat gtccgcaata atggcagcgc attgaacgtg tatccatatg
    11521 cagtgtaaga gagatatcgc aattaggaca tggaagaaca aatccacagt ccctacacat
    11581 cacaaaagac gaatatcctc gacgattgag cagtaggaca gtttgttctt ttttgtctaa
    11641 tcgattttgt attttttcct gcaaattagc agaaaaagta gaggtccgat gattctgaaa
    11701 ctcttcacgc atatctacga tctcaatagc tggaagctgt gcagctgctt tggctcggtg
    11761 gtttaactgc aataactgat aaacgccttt ttgagctcgt gctcttgatt ctaaagaggg
    11821 cgtcgcgctt cctagaacta ttgggcaatg atgatattta ctgcgccaaa ttgctagatc
    11881 tctggcatga taacgcggtg tctcatcttg tttgtaactt gattcatgtt cttcatcgat
    11941 aatgatcagc ccgatatttt tcagcggggc aaaaatagca gatcgtgcgc ctactactac
    12001 ttgcgcttct tcccgctcaa tcttgcgcca ttcatcatat ttttcacctt gggaaagtcc
    12061 gctatgtaag accgcaactg attctccgaa gcgtcctttg aatcgctcga ccatttgcgg
    12121 agtcaatgcg atttcaggca ctaacataat agccgtttta ttttctgaca aaacatcagc
    12181 gattgcctga agatatactt ctgttttccc gctgccagtg attccctcca gcaaaaagac
    12241 ctgatctttt tgctgctgac ctgctgtgat gatttgttcg acagcatttt tttgttctgc
    12301 attcagttcc aatgccgtcg tttggtcaaa tactcggtct ttataagggt cgcgataacg
    12361 ttctgattct ataaactcca gccagccctt ttcttttcct tgattcaaga cagcagtact
    12421 gattccttta tttttgaaat aagcaattgg ttcttcttgg ctcagttcag ataaacacaa
    12481 aagaagttct ttttgtttct tggcattttg ccgtaatttt gcccattctt cttcgatttg
    12541 cttttcagtt aacaaagaac ggaccaaacg aatcgttttt actttatttc gtgtatggac
    12601 ttcatatttg atatctacta attgctcttg gcgccagcga atcaattgag aaaggctgcc
    12661 ttcttcttct gcttccttcc aggaaatcac ttctcgctga tcaaacaata cctgaacttc
    12721 tggtgtgtct gccaaaggat agatcaattt atcatattct gctttcatca cactcggaag
    12781 catcgtttgt agacaagtga ttttgaatgc gtaagtaatt tttttcatat aatcagctaa
    12841 tgaaagtaat tcttcattga ccaccggagc aaggtctagc aaacgaataa ctgccttcaa
    12901 agatggatta gctgattctt ctgatttttg gatagccata acaaatcctt ggacatgacg
    12961 attgccatta ccaaaaggga cttctactcg catcccgact tgcaaagctg tttccatttc
    13021 agagggaacc aaataagtga atggctgatc ggtctgcata gtaggtacgt ccacaatgac
    13081 ttcagctgtt aatgtcatgg ttcgtttcct cctttttttg tcttttctta tcaagcgaca
    13141 aaaagttcct tgcctatttt actagagatc gatggatttg tcttatcttc tgccaaatcc
    13201 taacaaaaaa ttaacagcag ataagaaaaa aaagaactgt gtcaaaaagt tcagacacag
    13261 ccttttctac cagacaataa gtggcgaatg tttgaaaagg aaacagctgg gtttgctttt
    13321 ctttttagat gttcgctcta tttttcttta ttacattttt tgctcttcac ggatacgtga
    13381 ttccaattcg tgttgttcac gctctttttg tgcttgacgt tcctcttctt ccatcttcaa
    13441 acgttcacgt tttaattcag gatgtggatc attgactaca tttcctgcat cgatttcttc
    13501 taaagcttga ccgacgcttt ttactgaatc aaaagaatct agagtaggct gtgcacttgc
    13561 atccaattca tgcgcgcgtt tacttgctaa aatcactaga gaatattttg aatttacacg
    13621 atctaatagt gagtcaattg aaggttttaa catcataaga ctacatctcc tttaacattt
    13681 taatatattt accgattacg cgatctactc gaaaatgttc actggcaata atgtttttga
    13741 ttcgttcaac tgctagcggc acttcatcat ttacaacagc atagtcgtaa agagccatca
    13801 tttcgatttc ttcttttgca actttcattc gtttttcgat tacttcatct gcatctgttc
    13861 ctcggcctac gatccgtgat tttaattccg ctaaatctgg cggagtcaaa aagatgaata
    13921 caccatcggg cactttttct ttgacttgtt ttgctccttg gacttcaatc tccaagaaaa
    13981 catcttttcc ttcatccaat gtcttgttga catatgacaa aggagttcca taataattcc
    14041 cgacatattc tgcgtattcc agcatctctc cagcttcgat cattgattca aactcttctt
    14101 tgcttcggaa gaaataatca acgccttcga cctccccttc acgcatttta cgggtagtca
    14161 ttgagatcga atactgaaaa tcattatcct cactctcaaa aattgctttt cgtactgtgc
    14221 cttttcctac accggatgga ccagacaaca cgattaataa tccacgctct gacatgacga
    14281 cgtcctttca actagtcttt ttgtacttat cgtttatttt gcactttttt tctgcaactt
    14341 tcaaggttat ctttaattat agctagaaat tggtaaaaaa gctaccctgt accaagaggt
    14401 cgtaaaaaag ctgtcctgca tcaagtaata agaacagcca aacataaaca gcttctcatg
    14461 tttttcgttt ggttggggtt attatcgcag atgcaagctt ttgaacgccg tgtataagag
    14521 gtcgtgaaga agccgattag tttcgagtat gaagaaaaat tttgtgaaaa tagtttctca
    14581 catttttgca taaatttggc ttaataccga gaaaatggtt tttgaacatc acttattcaa
    14641 aataaaaaaa gattgtgaca cgaattttgt cacaaccttt aaatgtattc ctagagcaaa
    14701 gaagctaagc gaagaaatcc aattctgttt atttattgta tttctttgtt cagcatcctt
    14761 gctaaaagcc catattggac aaagtattca ctacacccat gatctcatct tttcgagaac
    14821 gtgctttttc tgtcggatct tctacctctt ctgtgtcaat tcgaacaata aatttccgat
    14881 tcatcgtcac gtaagcaccg tcgttgatca aaataaacag ttcgttctca ttatagtctg
    14941 cacaggcatc tttccatacg tcaaagccgt ttttttcaga agggatatcc cgtttaacca
    15001 cagtttttcc acttaattcg tggaacgtta cgtcataata tttcattatc tctacctctt
    15061 tcgtcattca atattttgta cttgttctct gattttttct aagatcgtct tagactggac
    15121 gacttgattt ttaatttcga ttgcatttga tttggaaccg atcgtgttga tttcccgatt
    15181 catttcttgg atcaggaaat ctaattcccg acctacagga cattgtgctt cgataagttc
    15241 atctagtttt tgtacatgga tcgccagacg atccagttcc tcgtgaatat ctccgcgttc
    15301 caacaagatg gctagctccg tcaacaaccg ttgctcatcg actttcgctc caagaaattc
    15361 ctccagcttt ttttgatacc ttatttgaaa ttccttttca tagatttcta caaaactatc
    15421 gagttttttt agtacttctt tgaattcttc agtattcttt ttcaacacta aagctaaagc
    15481 ttgaccttct ttttctcgac tcgcctcaat agaatcaagt gcgcttttca atgtatctag
    15541 agctaactgt gccaattctt ccatgctctc ttccggtttt tcttctaccg ttacgaagtt
    15601 ttcgtccagc acgaagcgtt ccgccatccg actaattgac acttgatttt gactaccgta
    15661 tcgttgttga attccgtctg tcaattgagt gataagtgaa tcaatcaatt cccagttgat
    15721 gacgatctgt ttttcgtttt tgccaatata agctagattc acaaatactt cgattcgacc
    15781 gcggcttaac tgctgtttaa taagctgacg aaaatcgttt tcaacaaagt tcaggatttt
    15841 aggacttcgt atttgaacat ctagaaaacg ctggttgaca ctttttattt caatatctaa
    15901 ctgatacgct tttgtttcgc ggatggcttt gccaaaacca gtcatacttt tcattcgaca
    15961 gggtccttcc tcattacata ttttctattg ctttttgcag ttgttcaaac tcttcgttcg
    16021 tcaatgtcag gcctttcccc attttttcat ggtctggcgc ccaatctcga agatcgaatt
    16081 ttggcgggcg accattccag ctgactaggt tcagttcttt acgccacccc ttgttgtttt
    16141 ctgacaatat agcgatttct tctacgattt catatgaaaa ttcttgtgcc atggttgttc
    16201 ctccttgaga taatataaat ataggatcag taatgtgtca actggttgct taagcaattc
    16261 atgctgcaaa cgatccttgc gttttccttg tagcagataa tcaaaataaa cagttttcgt
    16321 gcgctcattt tgtatttttt gctggtattt tcgatacctt cgccattctt cagtacttaa
    16381 ttgaccaatt tgtgccaata aatcatccgg catacttttt atatcttgtt ggcagatcat
    16441 ttgaatcatc atgtctactt gatagatttc attgggcagt aaaaaatgtt tttttgctaa
    16501 aagttctagc atcgccaatc cgcatgtctc tggtacgacc ctgttcttat ataaattacg
    16561 cagtttttgc caaaatttta tgcttttgaa aaaagataat tctaactgaa tttcttttgt
    16621 taagtaagac aaaaatcctc gccagtatgt tcttgcaggt ttaacagaat gaaatgtata
    16681 ccagttccca tagtaatagc ctaatcgttt tttcatttcc agatagccta attgtaaatt
    16741 taattggttc ctttggctat caaaaagcag aaagctgcct agtgtccaca atgtttgaca
    16801 tttccaatgg ataaaaatgt ctggcatacg aatttttttc gctgggcctg gcccttgcac
    16861 atctacgaca aatgcctctg ttgctccttt gttaatcgca atatcaatag gtattttgtt
    16921 gcgataccca ccgtctgcat atttattttt tccgatcttt cgataagcca tagcgggata
    16981 aaatgaagca gaggcgagta tccaatctgg tatttcttct gtttttgtct gttggatatc
    17041 agtcactact tccgctagtt ctggcagttt agtagacacc gtatataaac gtattttggg
    17101 attatgtttg agtaaggaca aatccaaatt gtctttgacc atcttttcca atggagcaga
    17161 agaaaccccg ccttcaatta gtgctgtttt cgtcatttgg cgtctttctc gatagaagcg
    17221 ttcacgaagg tcttcaactg cagccatttc tggtaacgca agcacttgtc ttgtcgacag
    17281 ctttttccat aaattcacag cttttttcat atctccttgt aaaatcaatg cgccgttcaa
    17341 cgcacctacc gaggtacctg tgataatctc aaagttgata tcatgttcct tcagcgcctg
    17401 ccacacacca atctgataag caccgtgcgc tcctcctcca cctaaaacaa gtgctgtatg
    17461 atagaccagc tttttattta attgtgtggt tccttgatag ccgtttttat gaaaaatacg
    17521 caaccattct tctgatagag gagattgaaa taaaaaacaa cagttttctt taataaaaaa
    17581 acgagcgcaa gcttccatat accgaaaaaa ttttttccat tcaaaagttt caccttcaat
    17641 caacagatta ttaaaaataa catctcctct acttttagca atagtaaaat agacggtata
    17701 ttttttcgat actgctttaa aagcacattg cttttttgca gaacgccaaa aagcgtctct
    17761 actttcttga tgactttgaa aaaatggaag gggttgtttt gcttgctgga taggcgaata
    17821 cagcgacatt ttctgtgaaa agtaaacttt ttttatctcc atcgcgctct cctttttctt
    17881 caagtatacc agtacgtcat aaaaaggtca tgagtctcgt attcgacttt gtagattcca
    17941 taagaaaaca attcataaaa cctcctttaa ataaaaaaat acaaaaatca caaaaattaa
    18001 ttgaaaatga gagtgttttc aatgaaaacg tgattttgta atggaatttt aaaataaaag
    18061 aaacaagctt ttttaatgca aatgttgtaa aataaaagag attaagagga aataagagga
    18121 ggaacatgat gactgagtca gtttatgtac atattgatac cacaagtaat gctgttttaa
    18181 caaaagggct gactgctact gactttgcaa acagtatcgt gcattttcct caaaacttgc
    18241 tcttgcttga tccatctgct tctgctggag aatatgaatc ccatacaggg ctgaaagtaa
    18301 tccgtggtac agaaaatatt cagcgtttct tttcctcttc tcgtaacagg cgtggattcg
    18361 atgaattgaa gtggatcgac tttactgatt taacgatgct gaaagaattg acacctttag
    18421 aaatttcaga attattatat tttggtcata tgaaaacaca tcttcattcg ccgttttttt
    18481 ataaactaca gaataatttt gtatacttcg atcttaatga tcagctgaat cggatttatt
    18541 atcgctattt agatgaattt tataggattt ttgctagtaa attaagttcg atcttgtcag
    18601 ataagatcaa tgtaaaaaaa tcttttttca aaaaagaaac acctgttgaa aaaatcagtg
    18661 tggaattatt gaaagaaatg cgtaatttga tgcaggaagg gatcattttt tcttttgatc
    18721 aagttggttt gatcgatggt aaatatcgta tcccaatcta tgtgattgaa gattatattt
    18781 ggaaattaaa aggcacccgg tatcgagaag aggaaatcat cgctacttta atctactctg
    18841 tcaccgatcg acactggcaa ttggtagaag atgccgatct ttcttatctg ccattgaagt
    18901 agatctattt cttatcaaaa aataaaaagg aaacaagaaa ctaggacatg attctgtctc
    18961 aagtttcttg cttctttttt atttacagct cctgtcgttt atctggcaca aaaatcgaag
    19021 gagtcgtttg ttggatccct aataaaacga tgatcgttac aatagctgta ctcaacccgc
    19081 tggcaccatt cattacgaaa gaaaagagcc aaggattcat cccccatagt gcatacgctc
    19141 cccagaaaac cactcctgca ataaaatgcc aaaaatatct ggaaagtgcg ccaatcaatg
    19201 caccaaacac gatgcttttt ctaatttgtt tcgtattatc gtctacatag cctttttgga
    19261 catttgaagc catgagccct gctgcgcctg caaaagtata ggcaatcggg tactcaatca
    19321 acacttgaac gacacttaaa taataaactt gtcccaacgg aaaatgtagg atgccccaaa
    19381 tgaaacctgc cagcatccct gcctttgtcc ctcttcttaa cgcaaaaaga gtcaatggaa
    19441 tctgccctaa tgaaatagaa aaactgctgc cgatctggat cggaatcaac gacaacacca
    19501 tcgctaacgc tgcaacaatc gttccttcta cccaaactct attttttccc ataaaaaaac
    19561 ttcctccttt gaataaaaaa gtcacaaagg aggaagcagc tttcgaaaat agtcaaacag
    19621 ctgtttattc acaattccta cgctcgcata accgatacag gtacgaaggg tttagaaata
    19681 gattcaatct cagcccaatt ggactcccct ttgtgagttt atttattatt tattttttgt
    19741 ttcaaacaat ggactaactg gtttgttttc atggatacgt ttgattgcat ctcccattag
    19801 tccgccaaca cttacttcat cgattttgtc gattttacga tcttctggaa gatagatcga
    19861 gtctgtaaca actagacgct cgatagctga atcttctatt ctttgtaaag cagggccgga
    19921 taatactgga tgtgtacatg aagcatacac actagttgca ccagcttctt tcaaagcatt
    19981 ggcagccaat gtgatcgtac ctgctgtatc gatcatatca tcgatcagta cacaagtttt
    20041 gcctttgact tgtccaatga tattcattac ttctgcaaca tttgcttttg gacggcgttt
    20101 gtctatgatc gcaatcggag atttcaaaaa ttccgctaat ttacgtgccc gtgtgacacc
    20161 accatggtct ggtgaaacaa cgacaacatc atcgcctttg attccttttt ctaagaaata
    20221 attggcaata agtggcgcac ccatcaaatg atcgactgga atatcaaaga aaccttggat
    20281 ttggactgca tgaaggtcca aagtcagcat tcttgttgca ccagcttttt gaatcatgtt
    20341 tgctactaat ttcgctgtga ttggttcccg tgcacgtgct ttacggtctt gacgcgcata
    20401 gccataataa ggcatgacta cgttgattgt ttttgcactt gctcgtttca gtgcgtcaat
    20461 catgatcagc aactccatca agttatcatt gactgggctg ctcgttgatt gaataacata
    20521 gacatgcgat ccacgaatac tttcttcaat gtttacttga atttctccat cactaaattg
    20581 agtaacagat gattttccta actcgacgcc gactgctgct gcaattttct ctgctaatgg
    20641 gcgattggaa tttaacgcaa aaatttttaa tcttgggtca aagtaatgtt ttgacatggg
    20701 gacctccact ttctttttca caactctaga attctaccta ataaatagta gtcatatttt
    20761 ttaaataaat caagtgattt tgcaaaagtc ttagtttaga taagggagtt tttttgcata
    20821 tccttcttta ttgacttgtc ttgctctcgc aattgccagc gcatattctg gaatattgtc
    20881 tgtaattgtt gacccggcag caatcgaact gtttttagct acctcgacag gtccgatgat
    20941 attcgtgctg gaaccaataa agctgtggtc gccgacagtc gtatgatgtt tgttctttcc
    21001 atcatagttg acgaagacaa caccacatcc gacattgatt tcttctccca gcgtagcatc
    21061 accaacataa gtcaagtgac cgactttagt tcctttgccg atttgagcat ttttcacttc
    21121 cacaaagttt ccgatatgga cgttttcgcc aatttcagct tttggtcgta aatgtgcata
    21181 aggtcccaca tctgcatgtt tcttgacatg gctgctttca atgactgagt tttcaatgac
    21241 gacgtgatcc tcaatcctac tgtctacgat ttttgaatgt gacccgatga cacaatcaga
    21301 accaatgact gtatttcctt ggatttgtac acctgcttcg atcaatgtgt ctggaccgat
    21361 ttctacgcct gcatcgatgt atgtcgttgc tgaatcgaca aagcttacgc cattgaccat
    21421 atgcatctgg ttaattcgct ttctcatgat ctcatttgct ttagctaacg cgatccgatc
    21481 attgactccc attgattctt caaaatcatc cgtttgataa gcagcaactg tatggccttc
    21541 ttcttttaag atttcgatga tatctgttag atagtattca ccttgagcat tgtttgttcc
    21601 aactttgctt aacgcatcga acaaagcttc gttgtcaaaa cagtaagttc ctgtattgat
    21661 ttcttgtaca agagcttctt ctggtgttgc atctttttgt tccacgattt tttctacgat
    21721 cccaatatgg tcacggatga ttcgtccata tcctgttggg ttttctgctt gtgccgtcaa
    21781 gatcgttgca cttgcatttt ttccttggtg atattcaaaa agattgttca gcgtttcagt
    21841 agtcaataat ggtgtatcgc cactgataac taacgttgtt cctttttttc cttccaagaa
    21901 agaagcagct tgcaaaacag catgtcctgt tcccaattgt tctgcttgta acgcatattt
    21961 actgcgttct cctagttgtg ctttaacttg ttctgctccg tgtccaacga tcgtaatcac
    22021 ttcatctggt ttcgtttcac tgacccgatt aatgatatgt tctaccatag gtttaccaga
    22081 aacgggatgt aaaactttat ataattttga cttcatgcgg gtacctttcc ctgccgccaa
    22141 aataattgca taacgtgcgt ccaactgcgt cactcctaaa tatagtttgc tgatttcagt
    22201 ttatcccagt atacccattt tttcaacaaa acaagtgatt ttcttttatc ccagcgtttt
    22261 ctggataaaa aaatagatag accataacgg gcctatctat ttggtaaccg gtgctctcct
    22321 gccaactcct cggtcacaag tcacactact cataaaacat gaaaagctgt tttttgaaat
    22381 cgtttcttgt gacttagagt taaacggtag gcttacaacc tcttcattaa acgtttttca
    22441 atcagctgac cttcggtatt agctcaataa acggcaaaat atgaggagca atttttccgt
    22501 ttattgagct aatactcagg acaggacgct gatttcacaa cctctagata aacggtgttc
    22561 aaaagccagt ttctcggtat taagccaaat ttatgaaaaa atgtggaaag ccattttcac
    22621 aaaatttttc ttactactcg aaactaagcg gctttttcac aacctcttat tctgaaaaat
    22681 aatttcctgg cacaaccgta atatttttcg tttgagtatc tacatctttg acataaagca
    22741 gtgacgtata atcatcgatg gcgcgatgac cgttgaattt tgattcggca aatacagtga
    22801 tgccaacaag ttctgattcg aattcttcaa tcaagctctt cattccgttg actgttccgc
    22861 cgcctttcat gaaatcatcc actacaagga cacgtgaacc acgtttcaaa cttctcttag
    22921 acagttccat cttttcaatt ctttcagaag atccagacac atagttgaca cttactgttg
    22981 atccttctgt tatttttgag tcccggcgta caataacgaa tggcgcattc agataatagg
    23041 acacagcttg ggcaataggt acgccttttg tggctacagt cattacagca tcgatttttt
    23101 caccaaggta tttagaagca atgattcttc caacttgacg cagcagttca ggttctccta
    23161 acaaatcaga gagataaaca tatcctcctg gaagcagacg gtcttgttct gatagacgtt
    23221 ctgctagaga ttcaatatat tttttcgcct cttcatatga aatttcaggg atgaacaata
    23281 caccacctgc agcacccggg attgtttcca aggttccata ccctcgttct ttaaatgttt
    23341 tcttgatgat tcccaaatct tcactgatag aagatttagc agattcataa cgttcggcaa
    23401 agcttgttaa ggaaatcaat tcatgcgggt gattcaataa atactgtgtc atgtcaacta
    23461 gacgttcact gcgacgtact ttcacctatt ccaactcctt ttaagaaatt ttatcgtttt
    23521 acattatata agtttttctt caaaaattcg agcattttat gaaaaaataa gaaaatagtt
    23581 cggtatttat taaaataaaa agaagcctat aaaaaatttt tcgtttttat aaggcttctt
    23641 ttctttataa tttagttatt ttttcttctg aaggcaacca atattcgttt gatgatattt
    23701 acaatcagga acaaagcaat aaagactaat gtgattgttg ctcctggagg tgtatctaga
    23761 taaaacgagc tagttagtcc tgacagcatc cctagaaatc ctacacaaac actgatagca
    23821 atcacgacat tgaagctttt gcctaatcgc ataccgattg ctgcgggaag aaccatgatt
    23881 gcggatatca aaagtgcacc ggcaatcggg atcatcacag caatagccac accagtaatc
    23941 acattgaaca tcatcgacat caaatgaacg ggtagcccat ccacgtgggc ggtatcttca
    24001 tcaaaagtca gaacatacat cggccgtttg aataaaaaga acaatatacc gatggcaacg
    24061 aataagattc ctaaaaagat gacttgctcc catgtaatgg tcacgatcga accaaacaga
    24121 taggattgga tacttgttgc tgaattccct ccgctcaaat tcatcaatac taacgctagc
    24181 gccaaaccac cagccattaa aatggcaatg gagatttctg aataggttcg gtagatcgtt
    24241 cgcagatact ccaatatcac agctgctaat acaacgacta gcatggtcgt aagtgtagga
    24301 ttaaggttca aaaagaatcc taacgctacc cctgctaaag agacatgaga aagcgtatca
    24361 gccatcagcg attgtcttct gatcactaaa aacacaccta gcatcggggc gattccggca
    24421 atgaagatag cggcaagaaa cgctcttctc atgaattcat atgaaagcaa cgccattgag
    24481 aatcctcctt tctcaccagt cggatctgtc tgtccgcata ctgtttgata tcttcatgat
    24541 catgggtgat cattagaact gatttcccat ggacatgcgc actatgtcgg agaagacgat
    24601 aaaattcatt tctggattct tcatccatac ctgttgttgg ttcatcaaga acaaataagt
    24661 cagggtcagt agcaaaaata cgcgctaaac tgattcgctg cttttgtccg ccagacaatt
    24721 cacctattcg ccgatttcgc atatcccaca tccctacagc ttctaaagca cgctgtacat
    24781 gttgatgatc tttttcagag agacgcttga accagcggcc tcttggatat cttcctgaac
    24841 ggactaattc gatcaccgta ctaggaaaac ctgcattgaa agatgcaact tgttgaggaa
    24901 tataaccgat acttaatttt tttccttctt gatttttctc agcaatcttt atcattcctt
    24961 ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt tgacttggct gctccatttt
    25021 ctccagttaa aatgacaaat tcacccgcat ctacatgata agagacatct tctagtacag
    25081 gttcatcatc atagtaaaag gtcaaatcag atatttcgat ataacgcacc ttacaacctc
    25141 cattctaaaa cttaattgat actttctttt aaagctttca gatttttttc catgacagaa
    25201 atataatttt cgcctgcttt gatttgttca ttagtcaagc tttctaaagg attcaacact
    25261 tctagcttca ctcctgtttc attcgacaat gtctcagcta ccttcgaaga ggcattttct
    25321 tcaaaataaa tcactttgat gttgtttttc ttcacaaatt cttttagctc agctaagcga
    25381 cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg ctacttgttt taagtcgtat
    25441 tgattcgcta aataagcaaa agcagcatgc tgagtgacaa atgtgcggtt tgttgcatct
    25501 tttagctcat cggagtattt ttgatccagt gcttcaagct ttttgatata agcatcggca
    25561 ttcttcgtaa aagtttcctt tttatctgga tatttttcgc ctaattcttc agcaatcgtt
    25621 ctgacttctt ttatagctaa taccggatcg agccaaacat gtggatcgta ttcatgtgaa
    25681 tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt cttgcgattc taaacggtcg
    25741 attcctttag ctgcttcgat catcaaggtc tgtttgctat ccactgaatc ctttaacgaa
    25801 tccacaaaaa attccatgtc tgaactatta tacacgaaaa catctgcatc ggaaatttct
    25861 gctctttctt ttgcacttgg ttcgaaatca tgcggctccg ttccagcagg gatcaaaagt
    25921 ttcacgtttc cttcatcccc tacgatctct tttgtaaaat catacatcgg ataaaaagtc
    25981 gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt tcgtatttcc acatgctgca
    26041 aacagtaaag cacctatcat taccgtagct gtcaaaagat attttttcat ttatatgctc
    26101 ctttcaatga acaaatcgga attttacgat ttattaacgt gaactaatgt atcaaaataa
    26161 aaaaacacag tcaatagaaa tccatcaatt tctgatttaa gcatgtatat tcttgattaa
    26221 atcgtaaaat ggatgagttg ctgctcatat ctagccattt cactacaata aaaaactgaa
    26281 gaataccatt ttaggattcc tcagtttttc atcacctatt ttacttctta tattttattg
    26341 cagggttcgt actaaataaa cttcctcaca aaaacctttt aacccattat atactctctg
    26401 cgctcttgaa taatgttgac acaaggcaaa aacggttggt ccgcttccac tcatcaatgc
    26461 agcatctgct ccgtacttga tcatacgctc tttgatttga tggatgactg gatgccgctt
    26521 tcccgttata tcttctaaac tgtttcccat ttttttgatc atctgctgat aatcttgctc
    26581 tcggatggct tcagcaagtc cgtggatgtc ttgatgttgg atggcttgcg tatctacttc
    26641 tctaaacact gtacgtgtag agacactcat tcttggtttg accaacacaa cccagcactg
    26701 tggcatagaa ggtaatactt ggatttcttc ccctttgcca ccgacaaatg ccgtacttcc
    26761 gtagacacag taaggaacat ctgtaccgac ttccaaaccg atttctgcca gttcttctaa
    26821 cgaacagccc agattccata attggttgat ccctcttaga gcagctgcac agtctgtact
    26881 tcctccgcct aatccagctg ctactggaat attcttctta acaaagatct tgacaccggt
    26941 tttgatattg tatttatttt ttagcaataa agcagcttga taaacattgt tccgttcatc
    27001 taccggtaaa aatgctttat ttgtttctac aatgattttg tcttctgtta tttcttcaaa
    27061 aaatagatga tctgccaagt ctacgctcga catcaccatt tccaactcat gatacccatc
    27121 ttgccgtcta tataatacat ccagtcctaa atttatcttg gctggcgctt tttcaatcaa
    27181 ttccatcctc tcacccattc gaaaagtcta agtcgcaagt attctatcat agggaaaaat
    27241 aaaagtatag cttatgtctg cctagtggtt gattttgtca tcgtttcaac agctttcaca
    27301 gaattttctc ttcataatcc aaacaagttc tagtgattct tataattctg tgagcacttc
    27361 ttctgccaaa ctcggaacat ttcctttttc ttttcctatc agactgagta gctgcatggt
    27421 agcaaatccg aaattacttc ctttcctata agcaaaataa cgtccttccc aatgatcgac
    27481 atattttaat ttcttttcat aagcagactg gaaggaatag aaaaaattac cataattata
    27541 tacgatatgc atgacttttt cttgaaagaa agaaaaatct gtttctccaa cattagctaa
    27601 tggagctaag cctagattgg cttccgagta gcccattttc tcgtatacaa taaacagatt
    27661 ggctaacaga tagtcagcta attcctcaga ttctttaggt aagacccgca acaaatcata
    27721 tcccgcctgc ttcttcgtca tcggctgagc ggtgatgaag ccaacgactt tttgatcttt
    27781 acggacaata ccgacaccgc tagataatac atatgattta tcaaatcgtc ccacagagaa
    27841 gtatttttct cgtgcacctt gaagccattt ttgtgaaact tcatttacag cttgatgtaa
    27901 ttcttccggc agttgatcgt accaagtaaa atggtatcct tctttcatca aacgttgata
    27961 ttcaagttgc tgactcagcc gagacaagtc ttctgttgct agatcaacaa tccctgcttc
    28021 acctactttc gtgaagtgaa aacccaattc atgaagcatc actacgtagc tttctgatat
    28081 cttataaaac gcaagctgat agcctaaaca atctgcttgt ttcataaact gattcgttgc
    28141 tgcccgcatc attttaggat tgccaactgg atctcctaaa acaaagcatt tgtttccttt
    28201 gatctgataa ccaaaaacga cttgatcttg gtcgtcttct tggtaataaa aataactgta
    28261 acctgacaag cgaagctgat ggcttgtagc tgtgcctccg tatgtttcca ataaacggtc
    28321 aaaacggtct ttttcgtact cactgccaag cttcgtagta tcttcagcta aatattgata
    28381 aagcgccact aaagctagca atgagatgcc caaaccaata agccctgaga accacacatc
    28441 atctgaagga aacaaaataa aatgatttcc tgttattcga ccgactaaaa agcgtccgct
    28501 gtgatagcct gcaattgcgt aaacgataaa caaaaagccg aacaagctag catcaaagat
    28561 tatagctccc caagaataaa cgaattttgt tcgataaaat tcttttctgg ataaccacac
    28621 agctaacaga atcaagacat ataccaatat caactgccaa gataacgtac gtgtgattgt
    28681 atttaaaata ccaaaagata gtaaagcaat cgtaggcaga aatgcctttt tgacttttac
    28741 tgaaagtgct ctagcaaggc cgagtaataa aaagccaacc aatagattca ctgtctgatc
    28801 taaaaaattg aatgagaacg gcaataatac ttgaaatagc cgactgattg cagacagatt
    28861 agtgattgtt gatagaagta ccatcatgat ccccgcaaaa tagacagcac ttacgagcac
    28921 gatatgcgca accttttgga aaaacagtcg cggaatgtta tctaaaaatc gattgatttt
    28981 gactcctgta tgtgtgatga acaatagaag tcccgtcata aaaggtagaa cataataaaa
    29041 caaccgataa tataatagcc aaactactgc tacatcttgc cgaacagaca attgactcat
    29101 tcctaagatc atcaaaacgt caaaagtccc cattccacct gggaccatcg tcagcattcc
    29161 tatcaatgta gcaatcatga acataggata gatcgaaagc atcgaaatat ctattctcat
    29221 caacttgccg atgatcaaaa ataccgcaag ggctcccgtc cattgtccaa aggatgccag
    29281 tagcagacca agcaagcctt ttggataaaa ctcgaagaac aatgtctttc tctttagata
    29341 agcaaaaaag aaaatgactg gtgctaaaaa actaccgccg aaaagccaga tccaatagct
    29401 tcgaaaaata ctttctgact gaataaaaaa gacatcaata aatgtaagaa acgaccatac
    29461 agacaaacca gtcagcataa atagtgcgac ttttgataca gtggctaaaa ccatttttct
    29521 atttgtctgc tttccataaa aactagcccg cagacttgct cctaccacac caccaaaacc
    29581 ggcgagattg ttgatcgtgt ttgttaccca cgcagcaaga agaaactctt ttctttccat
    29641 ttttggtttt ccttgttttt ctaagactct gatcgtcacc caatcataca acaacatcgg
    29701 agtgacaccc agcagtccag ccagaatcat gagcaggatc gtcatgcggc tttgttgtcc
    29761 catcctttgc caaatatctt gccaactcat gccattagca atatttgcga cttgatttgc
    29821 cacaaagact aacacggagc cgaaaaaaat cagttttaaa atcagactat gttcctttat
    29881 ccattgcagc atcaagtatc gtttattttt cacattttcc cattcctaac ttgtatactt
    29941 ttgtgtcata ccccaatgtt atcttattta ttttaatttt tccactttag acctgttctt
    30001 tgcttgcgat gatagaaaaa gaaagatatc ctaatgctaa aggagaggat aacatgaaag
    30061 taggatttat cggcacaggg aatatggcac aagctattat tgatggttta ttaaaaaaag
    30121 gaacagtctc gaaagaagag gtgttcgttt ccagtggaca ttttgaaaat gcacaaaaat
    30181 ttgctgctga aactggcgtc catgcatgca aaagtaatca ggaagttgca tcacaatcag
    30241 atatcgttct tttagccgtg aaaccaaaaa tcattgaaaa agtattggaa gaactaagag
    30301 aatatagcca gaaagtctta ttcatttcta ttgcttcagg aaaatcatta atagatttag
    30361 aaacagcgct tgaagatcct caggcctcca tcgtacgtgt gatgccgaat gtcaatgtca
    30421 gcgtaggagc aggcgtttct gcgatttgca aaaatgaagc tgccacggag gaacagcttc
    30481 agcaagctac gatgattttt gaagcaatcg gcagcgtcta tcctttagca gaatcagatt
    30541 tcagtacatt cattggcctt gctggcagct cacccgcatt cgtttacatg ctgatcgatg
    30601 cgatgggacg gactggtgta ctgaatggtt tgtccaaaca gacagccact gagattgctg
    30661 caaaagcaat tatgggttct tgtcaaaaat tcctttcaag tgataaaaat ccatgggaac
    30721 ttgtagacca agtttcttct cctggcggta caactgttgc aggtgtagtt gccttagaag
    30781 aagcaggatt catcccagca atcatcaaag gaatcaatac gacgatcgag aaagatcttg
    30841 agatgttgaa taaaaaataa tgatagcaat gttttttcgt gccttacaaa aaaagaagag
    30901 ctgtggcttt tgccgcggct cttcttttgt attttttatt atttagcata atcagttgca
    30961 cgagtttccc gaataacgat tactttgatg tgtccaggat aatccagttc ttcttcgatt
    31021 cgtttacgaa tatcacgtac taaacgaaca gcgtctaaat ccgagatttc ttctggcttg
    31081 accattaccc gaacttcgcg tccagcttga actgcgtaac ttgattctac accggcgaag
    31141 ctgttcgaaa tattttccag acttcttaaa cgacggatgt agttttctaa tgattcactt
    31201 cgcgcaccag gtcttgctgc tgagagcgca tctgctgcag ctacaaggac agagataaca
    31261 gaagtagctt ccacatctcc atgatgagaa gcgatcgcgt tgattactac tgggttttct
    31321 ttgtacttgg cagctaattc cgcaccgatc tcaacgtgag atccttcaat ctcatggtca
    31381 agtgctttcc caatatcatg gagcaacccg gcacgttttg ctaattggat atcttcgcca
    31441 agctctgctg ctaagatacc agctaatttt gcaacctcga ccgaatgctt caagacgttt
    31501 tgtccataac tagttctgaa tcgcagacgt ccgatgatct tgattagatc tggatgaagt
    31561 gtatgagcac cgacttcaaa cgctgcctct tccccgtatt cgcgaatacg ttcatccatt
    31621 tctttacgag atttctcgac catttcttcg atacgcgctg gatggattcg tccatcttgt
    31681 atcagttttt ccaaggtcat ccgtgcgatt tctcttcgga ttggatcgaa tccggaaaga
    31741 acaactgctt ccggtgtatc atcgatgatt aaatcgatac cagtcagcgt ctcaagcgtt
    31801 cgaatattac gaccttctcg tccaatgata cgacctttca tttcatcatt cggaagggta
    31861 acgactgaaa cagtcgtttc tgaaacttga tctgctgcgc agcgttgaat cgctaatgat
    31921 aataagtttt tcgctttgcg atctgcttct tctttggcac gctgttcaga ttcttttacc
    31981 attagagtta actcatgatt cagctcttct tcagtcgatt tcatgatgac atctcgtgcg
    32041 tcctcttttg ataaaccagc aattttttct agttcttttt gttgatcttc aatcattttt
    32101 tcaacgtctt tttctcgctc ttcaattaat tgctgttttg aactaagtgc ttcttcttta
    32161 ctttcaagtg agttctcgcg tttttctaac gagtcatctt tacgattcag cgtttgttcg
    32221 cgttgaatca aacgattctc ttgagatttt aattctaact tactttctct cagctcactt
    32281 tcaacttcag aacggtattt ctgattttct tccttagctt ctaacaatgc ttcttttttt
    32341 agagtttctg cttctttttt tgcactatcg atgattccag cagctgatga ttgtgcacca
    32401 gctatttctt tctcgtgacg tgactttgcg accataaacc ctaaaccaag accgacaatt
    32461 aaaccgatga tagcgaggag aatgttgaat accataaaat ccacctccat actatctttt
    32521 tcatttttat catctataca gcttctatga taaattattc taaactcgtc aatatgcatc
    32581 ttcgcatata tgtgtacaaa aaacatgcac aactttattc taatgtttgt accccgtagt
    32641 gtcaacttta aataaacaaa gaaacggctt tctacttatt cattaaacga tggtttttaa
    32701 aataaatgac actagtttca tataggtaaa cctttaaatt gagaaacttt tctattaaat
    32761 tattctttgt agatgaacag atatttttat tttatgtacc cactcgtata attaaataac
    32821 tcacgatttc tttattcaat aaaaaaacgg caggtcaagg agagtaccta ctcttcagac
    32881 cagccgtttt tgtttatcat aatttttatt cttctactaa aggcaattct tcttgtcctt
    32941 tttcatcttc tgggacagcg acttcttcac caataccata agctgctcta actagagcag
    33001 aaacttctgc catcatttca ggatgattag ccatatagat tttagcgttt tcccgtcctt
    33061 gaccaatccg atcttcttta taagaatacc aagcaccgct tttatcaaca atgtcttttt
    33121 ctactgccat gtctagaagt tctccttctt gtgagatccc taaaccatac atgacatcta
    33181 cttcagcgat tttgaaagga ggtgctactt tgttttttac gactttgatc tttgtacggt
    33241 taccaacaat atctgtacct tgtttcaatt gttctgcacg acgtacttcc aaacggatcg
    33301 ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc tggattccca aacatcacgc
    33361 ctactttttc acggatttgg ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac
    33421 cagagagttt acgcaatgct tgagacatca aacgtgcttg taacccgaca tgtgagtcac
    33481 ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg actactacga
    33541 tatctacagc cccacttgat actaaagcat cagcgatctc tagaccttgt tctcctgtgt
    33601 caggctgtga aagaagtagt tcatcgatat tcacacctaa tttttgtgca tattgcggat
    33661 ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc atttttttgt acttctgcaa
    33721 tagcgtgtag tgcaactgtt gttttacctg aactttcagg tccatatact tcgatgatac
    33781 gtccacgggg gtaaccgccg acacctaaag cgacatctaa cgcaagagag ccacttggaa
    33841 ttgtagagat ttgttggtcg attttttcgc ccaatttcat aatagagcct ttaccatagc
    33901 tcttttcaat ttttttcagt gcagcatcta aggctgcttt acgatcatct gccaatacga
    33961 taatcctcct tatatttaca aatcagagca acatttcatt atccttatca tatctttttt
    34021 agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat ttatcatttt cttaaaagct
    34081 gttttctcaa aagatcaaat ccttgcataa ccgcattata tctgatagca gcacgatctc
    34141 tggcaaagtg atatttttcc gaaaccacgc cgtctttaga tgaaatagcg atccatactg
    34201 ttcctgcaga ttgaccttct aacgtgtccg gtccagccac tccggtgaaa gctacactgt
    34261 aatctgttct tgctatctca cgtgcttgaa tggccatctg ctccacacat tctttgctga
    34321 ctgttccata ttctgctaat agctccggat caatctccag aaaaccagct tttgtttgga
    34381 gcgagtaggt aacgaatccg cctggaaata cttcagatac accagcaatg tcgcctaatg
    34441 cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt
    34501 tcagtagttc cactacgact tttgctagcg aattatcatc gccgtatcca taaaaatatt
    34561 cccctactcg ttcttggatt ttttcttcta atgctaaaag agcttgattc cctgcctgaa
    34621 catcatttgt tttcactgtc agtcgtaatg tcacttcatt tggtttggca tatggtgcga
    34681 tcgttggatt gatctgtgtc tcaatcagat cttttagctc agtgaccagt cgcgattcac
    34741 caatcccata aaagcgcaaa actttagaaa ttagtttttc ttcagaagga aaacgttcct
    34801 ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa
    34861 gaaggatata cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc cctgtacgat
    34921 ttggtagagg aaccccgcct tcgataatct gggactgttg cagattgttt ttggtcattt
    34981 ttcgatgggt tgtttcaaaa tatgccaata attttttgta tccttccgta ttttgtatga
    35041 gagacttacc gaggtgagcc gcagttgctt cttttgtcag gtcatcttct gtcggtccta
    35101 atccgccaca taagagtatg agttcacttc ttgtttcagc tagagcaatc aactcttcta
    35161 aacgctgctg attatctcct acaactgttt gataataaac ttcgatacct agatcagcta
    35221 gctgttctga caagaaagtg gcattcgtat tcacgacttg tcctagtaaa agttctgttc
    35281 cgacagctat tatttctgct ttcattactt cccgctccct tatatagtat acgctgtatt
    35341 tttatatttt tttatttaaa aagagggcaa ggagctgtga caaaaatctt gtcacagctc
    35401 cttgttccaa ataaacagtg ttctcctgcc aaaacctcgg tcataagtca caatatacat
    35461 aaaacatgaa aagctgtttt ttgaaattat cccttgtgac tcagagttag acggcaggct
    35521 cacaacctct ccactaaacg atacttacat tgatcctttg aacacaccgc tgtttttgat
    35581 gaagtaatcc acacctgaat agatagtaaa gatcagacaa gcataaagca tgatttgatc
    35641 tactggaata ccgatcaggc tgaatgggat attattgata aacagcaaga taatagcaac
    35701 catttgtgta gccgtcttga cttttcccgg ccaagctgca gccatgactt ctccatgttc
    35761 gacaagtagt aaacgcaagc ctgtgacggc taattcacga cagacaatga tcgctactac
    35821 ccaaccaggg gcctttcctt ggtcaaccaa catgataaaa gcagtcataa ctagcatttt
    35881 atctgccaac gggtccgcaa acttgccaaa attagtgaca aggcctcgcg cacgggcaat
    35941 ttttccatct aaccagtctg tgatactagc taaagcaaaa ataactgcac ctactagctg
    36001 tgtgacagcc agtgtggtgt ctcctacgtt aaattgtccc caatctaaag gtaaacctac
    36061 aaccaaaatg aaaatcggga tcatacaaat tctaagtaca gttaatttat ttggtaaatt
    36121 cacactatcg actcctttct tccctattct acgtgactat cagcgaagtt tccaccgata
    36181 ttatttactt ttccaaatga atttattcaa aggagtattt ttattgaaat tgatcttttt
    36241 gaattaacga agaaggaccg cgacacagat ttgtcacgat ccttcttttt tatacctaca
    36301 agtaaacaga tgcgtaaact aatttatacg atcattctgc gtattgaaga gtaaggttca
    36361 cattttttgg actctgattg ttttcccctg gattaactgg tacggattct ccattcgctt
    36421 tgatcgttgc attgcttgct gcaccaatcg tgatggtcgc atttgttgct gtttccggta
    36481 acacggtact ctgtgtttca tttgctgcca gtgtcccttg ataaacatag gcattgtcta
    36541 ctagcacacc taaccataca cgattgacag cagtaaattc aagggttaca ggcttggtag
    36601 cgtctgtgat atttactgcg atggcactgc cagaatcttc tcctgtagct attgccattt
    36661 tcttttcttc acttgatttt gttgttgatt ctactgtaga agactctttg gtctgcgtgc
    36721 tttgctctga ggaagaggac tcctcgactg atccttctac tttaagcgaa gaagctgtgt
    36781 ctccgattat tggatcagct tgacgatctt gccaagtcat atagcccacg acaacgacaa
    36841 tcgccaaagc aaccaatcct aataaaatga caggaagact tgtccaaaag cgacttggat
    36901 gcgattcttc ctgatgcatt gcttttctag aagtacttac tgtttcaggc tgcggacgtt
    36961 cagggaccga tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa tggtctccat
    37021 cttctcctac agcttcagcg tattgccgaa taaatgcccg cacgtaaaat ttaccaggca
    37081 attggtcaaa atcatcattg tcgattgctt ccaaataacg tttttgaatt tttgtaatct
    37141 gctgtaattc atctaaagat atttttttat tcaggcgtgc ttgccgtaat ttttctccga
    37201 tggtttcaaa agccactcgt tcatcttctc cagtctgttt gattctccga agcatctgct
    37261 gttgcaacga aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt
    37321 ttccgaataa acattgttct cctgccaact cctcggtcac aagtcacaat atccaataaa
    37381 tataagaagc tgttttttga acttgttcct tgtgactcgg agttaaacgg caggctcacg
    37441 acctctttct taaacggtgt tcaatcagct gttcctcggt attagctcaa aaaccggcaa
    37501 aatataagga gatatttttc cgtatgtttc tctaatactc aggtcaaaac gctgatttca
    37561 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt
    37621 taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    37681 tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat aagccgaaat
    37741 atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
    37801 ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag ttgattaaaa
    37861 gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag aataccataa
    37921 aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag acttttcctc
    37981 ataaaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct tcacttataa
    38041 atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat
    38101 caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg
    38161 aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt
    38221 ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt
    38281 tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga
    38341 attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag
    38401 aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt
    38461 ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg
    38521 taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac
    38581 gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca
    38641 tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa
    38701 acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc
    38761 ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca
    38821 tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct
    38881 tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat
    38941 tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag
    39001 cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa
    39061 acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac
    39121 catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct
    39181 tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat
    39241 aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa
    39301 aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg
    39361 ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc
    39421 aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc
    39481 agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct
    39541 aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg
    39601 acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct
    39661 ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag
    39721 accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc
    39781 agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt
    39841 tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact
    39901 gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt
    39961 gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg
    40021 actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag
    40081 gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg
    40141 ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat
    40201 aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca
    40261 ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat
    40321 aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgaa
    40381 taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct
    40441 tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt
    40501 aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca
    40561 cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg
    40621 cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag
    40681 tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt
    40741 attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa
    40801 agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat
    40861 tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat
    40921 aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct
    40981 tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat
    41041 tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa
    41101 gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta
    41161 tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt
    41221 agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa aagaaagccg
    41281 attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg
    41341 tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac
    41401 tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt
    41461 ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg
    41521 tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg
    41581 taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttc tccatcaatg
    41641 tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg
    41701 acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact
    41761 aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac
    41821 gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct
    41881 caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt
    41941 caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt
    42001 ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt
    42061 ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact
    42121 ataccacata tttttaaaat tttcaagttt accataaaaa aaaaaagaaa tgcaataaaa
    42181 ataaataaga agctcattaa gatttttcta aattattcct cattataaaa tttcaagtgt
    42241 ttttttattt tgagttttac tctcccgtaa gcgctcatta cttgcttgtg atccattgct
    42301 aaacattcag ccatgtctac aaaatcaacg ccttgcatat aataatgaaa aattttagct
    42361 tctatttttg aaaactggat ttcgcaattt tcgattgctt cttttaataa aagatgctga
    42421 attgctaaag gttcttgcaa tgtgatctct gctaaaaagt ctggtccttc tgtctctatt
    42481 ttttggtcga ttgagacttc atctttttga tcacgacgct tcagcgcgtt ctgcttcctc
    42541 aacagagaat agatttgatt gcttataacg cttttataat aacttccaaa gctaacacct
    42601 ttgctttttt cgtaagttct caatgcctga tataaagcga tccttgcttc ttgttcccaa
    42661 tcctcagaaa cataatttct taaataatag gtgtttttca aacgtagtaa aagtggaaaa
    42721 tatttttgat acagggcagc aaatgcttct tcgtcaccat tacgtgcagc gcgcaataac
    42781 tcctcagaca taaaataatt cctccataaa ttcgtttatg gaggaatctt atcatcaatg
    42841 attcatcgaa ctgattagaa cgatatgtta tttatttttt ctcattaaat cgtccaattt
    42901 ttgggacaat ttttttaact gctcttcatt ccatggagaa ttacgtcgga aatcaataaa
    42961 atgcatatct ttattttctt gggctattgt tttttccgtt ttttttatcg tcttatataa
    43021 ttcataagca gaagttcgta atgctccttg ggaaaagatc atccactgtt ctgctaaatc
    43081 gcttgtcgcc actgtcactt gtgtcaaacg gtcattcagc tgaccagcga ttctctcgat
    43141 ataactgtca gccgtttcgt cttctttcgt aaaaacaaca tccaactggt atttcttata
    43201 attttgttgg atgcccggaa ctagttgggc atcgaagaca acgatgactt ccattccttc
    43261 atatttcgca taatttgata aacgatgtaa aagagcttct cttgcatccg ccattttgtc
    43321 ctgcttttta agctggacaa gttccggcca cgaaccaatc atgttgtagc cgtctacaat
    43381 caacagctgt tttttcatgg tctactcccc ctgacgttgg gaaaaagcct gatacataag
    43441 caaaccagca gcaacactgg catttaagct ctggacgtgc ccggtcatcg ggatcgtcag
    43501 caattcatcc atttcttttt tcaatcctgg actcattcct ttgccttcat taccaatgac
    43561 taaagcaatt gatcctttgg cattccattt ttggaagttt gtgccggtca tatctgtgcc
    43621 aaatacccaa aatcctgttt ctttgagctg ttgcacagct tgtaccaaat tcgtcacgcg
    43681 tgcaacaggg acatgttcga ctgccccagt ggaagtcttt gttacgatag gtgtgattcc
    43741 aactgctcgg tgttttggta tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa
    43801 aatcgaccca aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga aggggtcttc
    43861 ttttttctct tttgttcgtt caatcagttg gtcaagggat aaatattcat aaggtgtaat
    43921 cgcaagaacg actccttgat gcacgccgtg atcgctcaaa gtttctaatt tttgtttagg
    43981 aacccatttg actggcacag cttgttcacg tgcgatttct ttcagttctt ctactttttc
    44041 tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc ccttgttgta gcgcttctac
    44101 tgcggcatgg tgcccaaaga cgaaattatc ttctaatgct tcttcatttt gatcagaaac
    44161 tgactgattt cgtccagttt gctttttatt tgaatatcta ttttgttttt tgtcttcaaa
    44221 tccacgtttc gattgatgtt tgtttgttct ttctttaccc atttttttca ccaactttct
    44281 gaatacacca gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc
    44341 ccatgagcgc ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca ttttttgcgc
    44401 tcgtatggct tttagcattt cttcctctgc gataataaag ttcttcttct tctgtcaaaa
    44461 gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa acataattcg
    44521 ttgccgcacg atgcagctta ttgggttttg tttctccttg atcaatcagg tggtcacgaa
    44581 catagatttc gtaaatcgca tctccaacat aagccaaggc taaaccgttc aattgtgtgt
    44641 aatctctcat gcttctcttc tccatcgggt tccttgcggt gtatcttcta ataagatccc
    44701 tttttctttc aacaaatcac gaatttcgtc gcttcggaca aaatcacgat tgcttcttgc
    44761 ttgatttcgt tcttcgatca attgttcaat ctcttcatct aataactctt ctgctgtaaa
    44821 taaaataccg aagatcgaaa gccattgcga gaataaagct tctccttgtg ccaagacagc
    44881 ggcagaaact ttcggctggt ctagataagt gttcagccac ttagcaagct catagaccac
    44941 tgtgatccca tttgctgcat taaaatcatc gtccatttct tcgatgaatc gctgttctaa
    45001 attttctaac aaagcgattt tatccgcatc atctgccaaa ttctctgagg cagtttcttg
    45061 gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg ctgcttcttt
    45121 catcgtcgtt tcactgtagc ggatcggacg acggtattgt gtggtcgcca tgaagaaacg
    45181 caagacttgt ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat tccctagaga
    45241 tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca tccagtaatt
    45301 agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcat gatgaggaaa
    45361 ttctaaatct tgcccgccac catggatatc gatcgtttct cctaaatgtt ttgttgccat
    45421 aacggaacat tcaatatgcc aaccgggacg tcctttgccc catggtgaat cccaagaaat
    45481 ctcgccaggt tttgcttgtt tccataaagc gaaatctaat gggtcttctt tttgctgctg
    45541 ttcttcccct gtacgctgac ttgctccgac ttccagttca tcgatggatt ggtcacttaa
    45601 tttcccataa ttttcaaatt tacgggtacg gtaatacaca tcacctgaag attcataagc
    45661 atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat gatccatgac
    45721 tcttggatgc agtgtagcag gttcgacatt caacgctttc gtatcttctt caaatgctcg
    45781 gatgaaacga tcagcaactt ctggtgctgt aatacctaat tcgtttgctg tacggatgat
    45841 tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc gatattcaaa
    45901 atagcgtcga attgtatcga aagcaatggt gctacgagca tttccgatat ggatataatt
    45961 atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag ggatgaattc
    46021 ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct cactttttct
    46081 cctttgattc gaacgacttt tgctggtact cctaccgctg tagcgtcgtc tggaacatct
    46141 ttcaacacaa cggcacctgc accgattttg gcgttttttc cgatcgttac cggtcctaat
    46201 atctgcgcat tcgcagagag catggctcct tgtttaacag ttgggtgtct tttacccgtt
    46261 tcttttcctg tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc ctcgatttct
    46321 gcggtttcgc caatgacgat tcccattcca tggtcgatga acacgccagt tccaatcgtt
    46381 gctcctggat ggatctcaac accagttagg aaacgccaga attgagcatt gatcttcgcc
    46441 aataaaaaga gccggtgctg atataaaaaa tgagagaaac gatgccaaaa caaggcatga
    46501 aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg cagccggatc gtttctcttt
    46561 gctgcatcaa tcgctcgggt aaaccaactc atgactttcc actcctttcg taagaatttt
    46621 atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt ttccttatca
    46681 aaagacgctt tccgcgtggt tccacttttc ttcataatca atgattatct ctgacagcta
    46741 acgcctgtta tacgttcttg gctactttgt ttcctataaa acagtttttc ctgttttctg
    46801 tgtttcgcca agaccactcc cagaggcatt tcggcaacca ttcattgata ccttgcacca
    46861 cccggtatct ctcttgtaat gatcagctgc ttactttttt ctgttcatcg tgtttagttc
    46921 agtacttgat ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat cgtttcaggc
    46981 aattctggac catgcatttg tcctgataca gctacacgga ttggcatgaa aagatttttt
    47041 ccttttacgc cagtttcttt ttgaacagct ttgatggcag ctttgacagt tggagcatct
    47101 actacttcca tttgttctag ctgtgctttg aatgcgtgga gaacagtagg aactgtttca
    47161 ccagcaagta cttcttttgc tgcttcattc aatacaggat gttcattgaa aaatagcgta
    47221 gacagatcaa cgatttctgc agcatagctc attttaggtt gatacaggct aacgatcttc
    47281 ttcacccatt cgattgtctt ttcatctggg ttttctgcta cacgtccatc agcgattaga
    47341 taaggcagac acatgtcagt aaatgcatct agatccattg ctttgatgta atggttgttt
    47401 acccattgca gttttttcgc atcgaaagct gcgggtgatt tacttaggcg ttttggatcg
    47461 aacattttga tcaattcttg gtgttcgaag atttcgtctt cgcccacagg tgaccagccc
    47521 aataatgcaa taaagttgaa cattgcatct ggtagatagc ctaattcgcg atattgttcg
    47581 ataaattgca agattgattc gtcacgtttg cttagttttt tacctgtttc actgttgatg
    47641 atcaatgtca tatgtccgaa ggttggtggt gtccagccaa atgcttcata gatcatcagt
    47701 tgttttggtg tattggcaat atggtcatct ccacgtaaaa cgtgagtgat tttcatcaaa
    47761 tgatcatcga ccgcaacagc aaaattataa gttggcatac catcgcgttt ttggatgaca
    47821 aagtcgccac cgatattatc ggattcgaac gaaatagcgc ctttcaccat atctgtaaat
    47881 gcgtattctg tattgcgagg cacacggaaa cgtacaacag attctagacc ttgtgcttct
    47941 ttttctgctt gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg aggcatttct
    48001 cctcttgcgc gttgtgcttc ccgttctgct tctaattctt cttctgtgca atagcatttg
    48061 tatgctcgat tgctagcaag cagctgatca atcagcggtt gatagatttc tttacgttca
    48121 gattgacggt aagggccgta ttctccagga ttttcaggag attcatccca attcattccc
    48181 aaccaagcaa ggttttccaa ctggcttttt tcgccatctt cgatattacg tttttggtca
    48241 gtatcctcaa tacgaataat aaagtctcca tcattgtggc gggcaaacaa ataattgaat
    48301 aatgcagttc ttgcatttcc aatatgcagg tgcccagttg gacttggtgc atagcgtacg
    48361 cgtactttcg tcattttctt cctcttcttt caaactagac tttgactaac agcaaaaatt
    48421 ccgtcagtta cggcttctca aatatcattt atttaaagcc actcagcaca aattttacct
    48481 tttctaaggt aaatagtaaa gccaactggt tgaatttcct taccagttgg ctgaatttct
    48541 tttttatttt gattgtttag tctttttctc tgtctctttt ttattgtcga ttcctcgtcc
    48601 tgcatgtgca ggtttagcaa agatcatgcg tccagcagct gtttgaaggg cacttgttac
    48661 aacgacttcg atacgttcat tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc
    48721 gtcatcaagg taggctactc cttgttggcg ttctgtcccg gcttttacta ctagaacatt
    48781 catcgtttca ccagggatga cgactggttt tacggcattt gccaacgcat tgatattaag
    48841 aacaggtaca ttttggaatt cagagacttt attcaagtta tagtcattgg ttacaatgac
    48901 accatctaaa agcttagcca atttgatcaa tttgctgtct acttcactga tatcttcaaa
    48961 gtctccatcg tacatttcga cagatatacc ctcttctttt tgcaaggcat ttaggatatc
    49021 taatccgcga cgaccacgca cacgtttcaa actatcccct gagtccgcga tgtattgtag
    49081 ttcgtaaaga acaaaattag ggatcatcaa ggttccttct aagaatccag tctttgtaat
    49141 atcataaatc cgaccgtcaa tgatcacgct tgtatccaag attttatatt tatggaaatt
    49201 ttctcctgat tttcgttcta acagttgact ctcaacttga ctagctgttt cttgctcgat
    49261 ttttttgctt cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg ttcccattct
    49321 caatcccaga tagcctaaga tgatcatcag taaaatcggc aatacactat tgacgaatgg
    49381 aatttccata ttgtaaagag gggtcgagat cacaacacct aaagtcaatc cgatgatcgc
    49441 accgatcgca ccgaagagaa gataagtcaa gctgacctca cttagttttt gttctacttg
    49501 tttgataata gctgagatat gtttggccaa cagcaaagat aataaaaaga aaataagtgc
    49561 gcctaacagg ctgttggtaa atgtattatt cagccatccg tttgactgtt gaccgattgc
    49621 ctgccaagct gagggtaaaa atgaaatacc caagcttgcg ccgacaatga tcatcagcaa
    49681 agtaatgaca cgtttttgca tcgtttttcc tccgtttcga caatcattta ttagttagcg
    49741 gaacacacgc ttcaacgttt cagccaaagt tgctactcca actatctcga ttccttttgg
    49801 cgcttcccag ttacctaagt tgtttttagg taggtatact tttgtgaagc cgagtttttg
    49861 gacttctttg actcgttgtt caatatgact gacacgtctg atttctccag tcagtccaat
    49921 ttcaccgatg aaacattccg ttggctgtgt tcctttttct ttatagcttg aagcaatact
    49981 gacagcgata gctaaatcaa tcgctggttc atttaatttt accccacctg cagcttttaa
    50041 gtaagcatct tgattctgaa gaagcaaccc agctcttttt tccagcactg ccatgatcag
    50101 agatacccga ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga acatagtagg
    50161 tgtgactaat gcttggatct ctaccaaaat tggacgtgtc ccttccattg ccacaacgat
    50221 agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag gattagcgac
    50281 ttcttccaat ccatgctcat gcatttcaaa aatcccaatc tcattcgttg agccaaatcg
    50341 gttttttact gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa aatataacac
    50401 cgtatccacc atatgttcta acattcttgg gccggcgatc gagccttctt tggtaacatg
    50461 accgacgata aaaatggcta tcccatttgt tttagcggtt ttgagtagtt ctgcagtggt
    50521 ttctcttact tggctgacac ttcctgccac acttgtgatg tctggctgcg tcatcgtttg
    50581 gatggaatca atgataacat aatcagggga gatatgttcg atggcacgac tgatttcgtt
    50641 catatctgtt tcagcgtata gataaaattc tgtgttgata cttccgaggc gttccgcgcg
    50701 taattttatc tgctctgcgc tttcttcgcc ggatacatat aagacttttc cgcctatggc
    50761 tgctaattgc tgagaaactt gtaacagcaa agtggatttc ccgatacctg gatcgccacc
    50821 aatcaagacc atagaccctg gcaccacacc tccgcctaac acacggttca gttcttctaa
    50881 ttttgttttg atccgaggtt cttttttagg gacgacgtct gcgatttttg ttggttttgc
    50941 tttttctcct gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt cgatttcttc
    51001 gaccatcgta ttccattttc cgcaatttgg gcagcgtccc aagaacttag gcgacacata
    51061 cccgcaattt tggcaaacaa attgtacttt tgattttttt gccattgtac cgcctccttt
    51121 tatcctaatc atgagacatt aaatcaagct taagtatctt taacgtttta tttactggaa
    51181 gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt gatctgcttt taaaaacggc
    51241 aaaaagatcc cttggccgat acgctcgcct tttttgatca ctacatcctt gaaaccaaag
    51301 ttggtgaatt ggaacatgat atggccttca tttgcctcat tattgtaata atcactgtca
    51361 atcacaccta ctccgtttgt caacactaaa aaccgcttca acggattgct tgaacggtta
    51421 gcaagctgca aaaattcatc ttctcccatg taagctttga ttccagtagg cacgagggtt
    51481 ggtttcaatt gtttttcatt ttctgataca tcttttttca atccatctgc gactgtttcc
    51541 aacccctttt tccagcctgc tttccagata cttggaatca cgacctcttc tgctgcttca
    51601 aaatcatagc cagcagcatg atgtgtagcg cgctgcggta gattgatatt ttgatcttta
    51661 tatttagaaa taacttcaaa tcctcttttt ctcaccggtt ttcctcccta aataaactac
    51721 tcctattcta acatatccga ggctgtaaaa gactttcttt acaaattctt caagaaattc
    51781 tgctatctta aagtaaacca ttaattcgtt ctttttccaa acgactgcaa gttgtacaaa
    51841 aaaactattt tttacatgac tttactcgtc aaaacaagta gaatagaacg aaattaatga
    51901 gtggatattt gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa acgaattatt
    51961 gtcaaagtag gaacaagtac attgatttat cccaatggaa atatcaatct tgcggcaatc
    52021 gatcagttag cttttgtcct ttctgatctt agaaatcggg gaaaggaagt gatcttagtc
    52081 tcatcaggag ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg tccgactttg
    52141 attcctgaac agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa tatctataat
    52201 caaagattca gtgtatataa tcaacagacg gcacagatcc tcgtgacaag agacgtggta
    52261 gattatcctg aaagtcgtaa aaatgtgagg aatacattgg aacagctttt gattatggac
    52321 acgatcccta tcgtgaacga aaacgatact gtagcagtgg atgaattaga tcatctgaca
    52381 aaatttggcg ataatgatca gctgtcagcc atcatggcaa aacttgtcgc ggcagacctt
    52441 ttgatcgtcc tttctgatat cgacgggttt tattcagata atccgactac aaatcccgag
    52501 gctcagatgt atcgctatat cgataaaatc gatgaaaaga ttctggcaca agcaggtggt
    52561 gctggtagca catttggaac aggcggtatg cagagtaaat taaaagccgc ttctaggata
    52621 tttgaagcaa acagcgcaat gattttagca aatggaaaaa atccggcaat tatttttgat
    52681 attcttgcag gaaaagaagt aggtacttta tttaaggagg acttgaaatg actgacttga
    52741 tgcaattagg acaacaggca aaagatagcg cgcaacaact agcacttatg cctacaaacc
    52801 gcaaaaatga gctacttgtt caaatggcac aaaccatcaa aaagaatcaa caagcgatca
    52861 ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa caatatcagt gaaacgatgc
    52921 aagaccgctt gcagctgaca cctgagcgaa tcaatgccat ggctacagag atcgaaaaaa
    52981 ttgcttcgtt agacgatcct attggaaaag tagatgagat gtggacgaac accgacggat
    53041 tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg catcatttac gaatctcgtc
    53101 caaatgtcac gacagatgca gctagtctag cattcaaatc agggaatgcc gttatccttc
    53161 gcggtggcaa agaagcattc ttctcgaatc aattattagt ccaattattg cagcaagtac
    53221 tggtcagtgc tggcgaatct ccttatgcca tccagtttgt cgatgatact tctcatgaaa
    53281 ctgcaggaca gttgatgcga ttgacagaat acttagatgt gctgattcct cgaggcggag
    53341 caaaattaat ccaacgagtc aaagaacagg caacggtacc tatcattgaa acaggtacag
    53401 gaaataatca cgtatatatc gataaagaag cacagttatc tatggcagtg aacatcatcg
    53461 tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc agaaacattg ctgatccatt
    53521 ctgagatcgc accttttttc ttgccagcta ttgaaaaaga actggtagaa cacggggtct
    53581 ctttacgtgc ggatgctcgt gcgttagaat atctagagac ggctgcttta gcagaagaca
    53641 cagattggga tactgaatat ctagattata tccttgctgt caaagtggtc gattctttgg
    53701 atgaagcaat tgcacatatc aatcggcata atactaaaca ttcagaaacg atcgtgacag
    53761 atagttatgc ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct gtctatgtca
    53821 atgcctccac tcgttttaca gacggatcgg tatttggttt tggtgcagag attggtatct
    53881 ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa tgaattaact tccacaaaat
    53941 atatcattta tggcgatgga caaatccgcg aataatgttt cgtgtgaaac attattcccg
    54001 ccttttgcct tttttctgta aaggttctat ccaactagaa aggtgtcaat atgtttgaaa
    54061 ttttaatgat tttttttatt taccttattt ctctgaatat cgctgctttt ctgggagtcg
    54121 gtattttatc ccttttcttt caattcaaaa aacgaagcat agggagtcaa cgggaaaaat
    54181 ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt tacacgcttg cacattagtt
    54241 acatggttgc attatgctta ttagcgacaa tcaattacta ttcttttttc gatcattcaa
    54301 tcgcttatac gattacgcta ctgattgctg ggatttttca cttatcttat aaatatcagc
    54361 tgaataaaaa ccacttgaat cggactttcc gctaaaaaat atccgtacga taagtagagt
    54421 gccttgaaga tcggcaaaaa actgccggac tttttagagg ctgtgacaaa gattttgtca
    54481 cagcctcttt ttccatataa acggcgttcg cctaccagct gcctggtaaa tctgtttcct
    54541 cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc ataataacga agcagctcat
    54601 ccatggacaa gttcatcgtt ttttcttttt cttcttccgc tgattcagcc agtagctgtt
    54661 cccgatacac ctgatacata ccagctattt tttgctgcat cgcttttcta gtttcacctt
    54721 ccgcattgat tattggcaat tctttgatat agccacgata aatcaaccga tcatctgaaa
    54781 attcaatggg taattcaacg agtgtcaacc cgacttcctg aaaatattct tccagcatct
    54841 attatccccc cattcttttt cttaaatgaa ttttagctta cctactgaaa attttccagt
    54901 ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc cctttttccg
    54961 aataaatggt gttcttctgc caactcctca atcataagtc acaatattca acaaacatga
    55021 gaaactgttt tttgaaattg ttccttgtga ttcagagtta aacgacagtc tcacaacctc
    55081 ttggtaaacg gcgttcaatc agctgaccct cggtattagc tcaaaaaacg gtaaaatatg
    55141 agaagctatt tttccgtttt gtttctctaa tacttaggat aaaacgctga ttttacaacc
    55201 tctgttaaac ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat
    55261 gaggaactat ttttccgttt gtttctctaa tactcaggac aaaacgctga ttttacaacc
    55321 tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    55381 tatgagaagc tatttttgtt tggctgttct tatgacttga tgcaggacag ctttttcaca
    55441 acctctgatt tcttattctt cttcatttgt agcaaaataa ttacggtgat aaatgataca
    55501 acatacactg ataagaacaa tggcacctgc aaaataaaac gcatagcttt caccaatatg
    55561 ttcaccaatc acacctgcaa taaatggtgc taaagctcca ccgaaaaagc ggacaaaact
    55621 atagctggac gaagcaacgt ttcgttgcag acctggtatt tccatagcaa tcgtcgtcaa
    55681 cagtgtattg ataattcctt ggaaaaatcc tgctaacaca atgctgatcg gtacaaggac
    55741 tggcatagaa gttccagctc ctaataacag caaacaaata atgaataaga caaatgctgc
    55801 taaaattgtc cgataagtcg taaacttccg ctcaagagcc ggtgcaacga aaatcgaagc
    55861 aatagccaga agcacacccc agccaaaaaa tacaaaaccg acttctaatt cagaatagcc
    55921 aatcaataaa aatggcgcat acgcaagtaa agtaaagaat ccgaaattat ataacaatgc
    55981 gattagtcca actgaacgaa gtttatgatt gcttaacgct ttgacacctt cccaaaggcc
    56041 tacttttctc tttggtttct cagtttcagg taaaagtacc gctagggcta taaaaccgca
    56101 aaacatcaaa aatgctaccc caaaaaatgg ataacgccat gaaccactgc ccaagacccc
    56161 accgactaga gggccaacag ccattccgca gccaagtgct gcttcataca tcatgattgc
    56221 tttttctgtg ttaccaacta aaatactgac gatcgttgct aaagcagtag agacaaataa
    56281 agcattcccg acaccccagc cggcacgtaa cccaatcagc attccgatat tcccagataa
    56341 tccgccaaga ccagcgaata caacgatgat aaacaatcct gtcatcaatg tttttttcaa
    56401 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
    56461 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
    56521 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
    56581 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
    56641 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtca
    56701 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
    56761 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
    56821 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
    56881 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
    56941 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
    57001 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
    57061 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
    57121 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
    57181 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
    57241 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
    57301 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
    57361 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
    57421 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
    57481 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
    57541 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
    57601 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
    57661 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
    57721 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
    57781 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
    57841 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
    57901 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
    57961 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
    58021 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
    58081 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
    58141 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
    58201 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
    58261 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
    58321 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
    58381 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
    58441 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
    58501 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
    58561 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
    58621 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
    58681 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccag
    58741 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
    58801 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
    58861 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
    58921 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
    58981 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
    59041 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
    59101 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
    59161 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
    59221 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
    59281 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
    59341 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
    59401 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
    59461 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
    59521 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
    59581 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
    59641 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
    59701 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
    59761 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
    59821 atagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
    59881 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
    59941 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
    60001 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
    60061 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
    60121 atcttccctg attcgtaagc ttggttgtag atttcttcgc catattcgta acattgcata
    60181 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
    60241 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggtttc
    60301 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
    60361 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
    60421 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
    60481 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
    60541 cggggatagc acggaacctg tatctttatg gtccatttca caatgcttga ttttcttctg
    60601 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
    60661 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
    60721 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
    60781 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
    60841 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
    60901 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
    60961 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
    61021 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
    61081 tgattcttat caaactatag aagatagctc ttctgcagta gataatttta caccgccaaa
    61141 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
    61201 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
    61261 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
    61321 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
    61381 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
    61441 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
    61501 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
    61561 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
    61621 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
    61681 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
    61741 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
    61801 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
    61861 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
    61921 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    61981 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    62041 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    62101 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    62161 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    62221 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    62281 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    62341 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    62401 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    62461 tgtattttct gagacagtag ctgactgcaa agtactttct gtatcgtttt caattcctgt
    62521 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    62581 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    62641 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    62701 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttaataa atttgctttt
    62761 tctgcataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    62821 cgtttgaata actatttaac aagtaattct ctctggatat tatccgttat ggattatact
    62881 tcctgtgtcg tcggacggat aatcatgttg ctgacagaca tccggtctgg cgtttctatc
    62941 gcaaatgcta cagcagaagc tatatcttca gatgttaatc cccattcttt ttgcgcttca
    63001 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    63061 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    63121 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    63181 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    63241 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    63301 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    63361 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    63421 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    63481 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    63541 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    63601 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    63661 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    63721 cctgcaacga tggtattgtc tgggacatct ttcgtaacag tgcattcgct gcaacaatgg
    63781 cattctcgcc aatcgttacg cctggcagaa tagttgctcc tgctccgatc caagcatttt
    63841 tcttgatgtg gattggcttt actgacaagc ctcttcgttc ttttggatcg gttagatggt
    63901 tcaccgtgat gaggttgaca cgaggaccaa tcaatactga atcttctaaa cagatgccac
    63961 ctaagtcaac gaacatcact tgacgattga taaagacatt tttccctata ctaatatgtt
    64021 tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt atcatctact ttgtcactaa
    64081 tgatattttc tagccgtttg cggatatctg ctggttcatg gtacccagta ttcagcgctg
    64141 ctaaaggctc ttcgttttcc gcttggatct gatggatcac ttcaaagagc ggatccgtta
    64201 ctcgaatctc tttattgacg atttgttttt cgatgaggct tgtcattttg ctccctcctt
    64261 ttatccttac tttataacgg acggcttatg atgtctaata cttataaaac atacttaact
    64321 attcctttta actatactta gtggtatgct tttttaaaaa caggagggaa aaaatggata
    64381 ttcgtatact gacgtatttt ttagcagtag ctagagaaaa aaatatcagc cgagcagcaa
    64441 aatctctgca tatcacgcaa cctactctct caaaacaact gaaagattta gaagaagaac
    64501 ttggtgtgac acttttcact cgaggctccc gagagataca gttgacggat tctggtcgat
    64561 atctttattc aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg aacaatttaa
    64621 caaatgacgc aatcatctct ggagaaatct acattggcgg aggcgaaact aaagcaatct
    64681 ctttcatttc tgatagcctt cattcgttga tcaaggcgca tcctgatatc cagtttcatt
    64741 tatatagcgg gaatgcagat gatatcatag acaaactgga tagcggatta cttgattttg
    64801 gactcgtaat cgaaccgaca aacaaacaaa aataccagtc atttcgactg ccagcccaag
    64861 atcgttgggg acttttgata catcggaatc atcctttagc aaagcaaaca gtcatccatc
    64921 cgcaggaact gaaaactgtt cctttgatca tttcccggca aacgtcagtg gacagtcaac
    64981 tagctgaatg gctaggtttc agcatagatc agttatcgat cgtcggaaca tataacttat
    65041 tgtacaatgc ttctttgatg gtcgataacg ggacttttgg tgcattagct ctagatggga
    65101 tcatcaatac ttatgggacg gaaaaagtat tcatcccact cgatccgcca ttacaagctt
    65161 cactgaatct catttggaaa aaagaccagc ctctttctcc tgctgctcgt aaatttttag
    65221 atgtcctaac ggagaaatac agttaagtag agtttttttc cttcgctttc agctttttaa
    65281 caacgaataa gttaagcaaa agatccaatg attaaataac ataagtttct taatttgttc
    65341 cttcctttta tttctgataa ttccagtaaa ttactataat tataaaaaaa gaattgacaa
    65401 aacataactg tctatataaa attgattacg ttaacaataa ttcgtttcat gtgactagat
    65461 catactaggc atgaaaaatc tctgaggaac atggtttccc taggaggttt ttcgtgtcta
    65521 tttttgtttg gccgcaatca actggtctag caaacgtgaa aaccaaatct tcaaaaaggg
    65581 tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca gtattggtag atgacggtgg
    65641 acaggaaaaa gtagctattg gaaaagggat tggatttgac agaaaaagaa atgatttgat
    65701 ttttgatcgt gatgtcgaac ggatatttgt catggaacca gaaggtcaaa tcaagttgca
    65761 aaatttactt aatcagattg atgaacgctt tttatttgca gcagaaacga tcattgatca
    65821 tgcggaaact gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca
    65881 cattgctttt tctgcggaga acattaataa cggtatcgtc atccgcaaca aacttttaag
    65941 agaaatcgaa gtgctttaca gtgatgaatt cagtattgct caatgggcag tagagtatct
    66001 gacaaaagag cttgggattc catatactta tgacgaagca ggatatatcg caatccatat
    66061 tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag tgacgattgt
    66121 atctgacatc atccatttag tggaatcaga attggaaaca gatatccatt cagaaccatt
    66181 ttcactgaat tattcaagac tggtgaatca tttacgactg cttctacaac gaacccatgc
    66241 gcaacaatat gctgttttag acacggaaat cgtagaaatg gtcaaacgaa aatacccaga
    66301 aagctataag atttctaaaa aaatccgtgt gttgctgacc aaagaatacc agctcgcgat
    66361 cacgaaagaa gaactgggtt acttggccat ccatatcgag cgtttaagaa gcgcaatcgt
    66421 acaaacaaat gttaacaatc atgaggagga aaaaaattga aagcatatct acaacgaatg
    66481 ggacgttccc ttcaacttcc agttgcggtc ttacctgctg ctgctttact agtagggatc
    66541 ggaaactggt gggcatctta tagtaacgac attgtcgctc attttttaca agctggcggt
    66601 aacgcagttt taggtcaatt acctttgctg tttgccgttg ggttagcact agggatgtca
    66661 gtggacaaat caggtgctgc tgccttggca ggtttggtcg catttgaagt tccagtaaat
    66721 gttttaaaaa ctgattcagt agctacttta ttgaatatca aaccagaatc agttgatcca
    66781 tcttttgctc agatcagtaa cgtatttatt ggtattattg ccggattgat tgcggcagct
    66841 ctttataatc gtttccacga agcaaaatta ccgatggctt tatctttctt tagcggcaag
    66901 cgtctagtgc caatcttggc tgcctttgtc atgttgctga tcagtgcagt gctactgctc
    66961 gtatggcctc cggtttactc tgctttagtt tcttttgcta aattcatttt gagtttaggc
    67021 tggggtggtg caggtcttta tggcttcttc aaccgtttac tgatccctac aggattgcat
    67081 caagcaatga actccgtgtt ctggtttgac gtagcaggaa tcaatgatat tggtaaattt
    67141 ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt tttcccaatc
    67201 atgatgttcg ggcttccagc aggagcatat gctatttaca gaaatgcacg tccagaaaac
    67261 aaagcaaaaa cagcttcctt gatgttagca gcagcttttg cttcattctt tactggtgta
    67321 acagaaccac ttgaattctc atttatgttc gttgcttggc cattatatgt cttgcatgca
    67381 attttcactg ggatttcttt agcagtgagt gcattcttcc attggacagc aggatttgcc
    67441 tttagtgccg gatttgtcga ttacttccta tctttgaaaa atccgattgc gaatcagcca
    67501 ttgatgttga tcgtccaagg attagtaaca gcagtgattt actactttgg ctttaacttt
    67561 gctatcaaaa aattccatct gatgactcct ggccgcgagg aagcagatct ttcagatgac
    67621 gatactgcaa cgactaattc agacaataaa tatgcggcac aagcaagcaa aatctacgct
    67681 gcattaggcg gagccgacaa tgtaacttcg atcgataact gtactacacg ccttcgtttg
    67741 caagtcaaag atacaggcct gatcgatcaa aataaaatca aagccactgg cgtacctggt
    67801 atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt
    67861 gcagatgaga tggcaaaact acatggtggt gctgcaacac gacctgcaca aacaaacacc
    67921 gtggttaaaa cagaaacatt tgccggtgga acgatcgaac aagatattta cgctatcgca
    67981 aatgggaaac tgattccaat cactgaagtt tcagatgatg tattcgcgga aaaaatgatg
    68041 ggtgacggct atgctgtatt accagaaaat ggagaaatct tctctccaat cgctggtacg
    68101 atcacaaaca ttttcccaac gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa
    68161 gtcttgcttc atatgggcat caacactgtt gacttaaaag gcgaaccatt cactttatat
    68221 gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg ctttagttga tcttgccgct
    68281 attcaatctg caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc
    68341 caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt
    68401 tccaacaaag cctgagccat cgtcttcccc agactttttt atcgataaac aaacaggagc
    68461 tgagacaaaa atcttgtctc agctcctttt tccgaataaa aacggcgttc tcctgccaac
    68521 tccgcggtaa taagtccagc caaacgtaac atatgaagag ctatttttta tttgactctt
    68581 cttattactt gatgcagaac agctttttta caacctctga taaacggtgt tcaaaaacca
    68641 gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa agccattttc acaaaatttt
    68701 tcttcatact caaaactaat cggctttttc acaacctctt tatacattat tcctgatcca
    68761 atatttcttt taatgctgct tcaagagaag ttaatggttt tcccaatact ttttctaaat
    68821 catcagagga aatagctaac tgatcctttt taatatcatg ttggatcgct aaaaacatgt
    68881 cagctgcttc ttgagggaat ccggcttctt ttagattttc gacaaacgct gcatcacttg
    68941 cttcaataat ttctaacttt ttacctgtcg cacgctctaa tgccttggct aattcttcat
    69001 agtcagttag attgccggat aattctaaga tttctggaga gtcactttct actagtactt
    69061 ttgctgcagc ttctgcatat tcacgtttca atgcccaacc tgtttttcca tttcctgcag
    69121 cataaacgaa ttgaccagtt tttaaagcct gcccaatgat aggtaattca ttctctacat
    69181 accaattatt ccgtaaaaat gtatgggaaa tacctgaatc cttaatcatt ttttcagtga
    69241 attggtgatc tggcgctaat acactgatcg agtgatcggc atctgcaaaa cttgtataag
    69301 cgatataaga taccccagct gcttgtgctg ccttcactac attcttatgt tcggcttgac
    69361 gatttcctgg aacgccagag acaaaaagca atcgatctat tcctttcaac gcttctttca
    69421 ttgagtctaa atcatcataa tcaccaatac gtatattaaa tccagcttct ttcagtgctt
    69481 ctcctttttc ttcactacgt actagtacat agatttcatt ttgtggaacc atctttttta
    69541 agaattccag cgcataacca ccaaaacctc cagttgctcc tgttactaaa taatgcatat
    69601 ttgccactcc ctattctttt tatttgttaa gatttacagc tgtacattta taaattacac
    69661 ctttatcata aagagtgtgg taagataagt caagcaaaaa aaatgaaagg atgacaactg
    69721 atgaagtatt ctctgcaatt tagtgatgca atccatgtac ttgcctatat tgaaatgttt
    69781 caagataccc gatcactttc tagtgagatg attgccagta gtatcgaaac aaatgcagca
    69841 aatgtacgta aaatcatgag tcagctcaaa aaatccggtt tgatctttac aaaaaccgga
    69901 aaaccgcagc cttcccttgc caaaagtcca gatcagattt ctttactaga tgtctatcgc
    69961 agcatcgaag gtaatacaca gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc
    70021 gttggtgcga atatccaaga agctttagaa gaaagctacg ctcggctgca gaaaaaggca
    70081 gaagaagaaa tggctaagat cacacttgat agtctgatac ataaaatagc cgttttggaa
    70141 aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat ttttataatt ctctctactg
    70201 tgcctgcatt tttctgatcg gaatcgtcta cataaattct tttattctcc gggggacaaa
    70261 agccttgtca tgcccctttt tcctctctaa tgctcaggtc aaagcgctga agtcatgacg
    70321 tcttgataaa cggtgttcaa gtagctgacc ctcggcatta gttctgaaaa atgcaaaata
    70381 tggaaagcta tttcccattt ttcctctcta atgctcaggt caaagcgcta ctttcacgac
    70441 ctctatatta tcatggttta ttgactaccg gtatccagat ttctcccata tatttcccgt
    70501 tggtaactcc catttcgact acagcatttg gacccccaac ataggcgtaa tcttctactt
    70561 ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt tcttaattct tcaggcgatt
    70621 ctgctaaagc gattacaaca agatacgttc cttttgggaa tgggatgatt cgggtagtgt
    70681 ttggaacatc ctgactcgtt tgaacaccga catagtacat cattttcccg ttatagggtt
    70741 cgttgactgt aaagaaataa tgattttcag cagcttgttt caatgcatct attgttccat
    70801 taccgataac tttttgctca aatgcttgtt tctctgcagc caaaccgaca aaatccgtat
    70861 tgctactctt caattccaca ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc
    70921 tatactttgc catttctccc accctttcta ttatattaag ttcatcttac ataaaattaa
    70981 agggaaatga cacgattacg ctcttttttc tagggatatt tttatttttt aactattttt
    71041 ttgctttcac tgcaatccaa atctcactgt aaacattact gaaatcctct ccccattttg
    71101 taaaagagat ctcgggagct tcgactaact cataatctga agaaggaagc cattctgcat
    71161 agattttccc ccaagtatct tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg
    71221 cccaagtgtg ttcttctata gcaagcattt ctaaatcagc gtacggattt tcttttgtcg
    71281 tagcaaagcc aatcatgtgc atcaaactgc ccttttcttc caagcgggct ccatcaaatc
    71341 cataagaaac gttgattact tgatttggat ataaatctcc taacttatgc atctcttccc
    71401 tttgagtctc cgtgatcgtc tgcgctaact tttgtatttc ttgattgact ccttcaaatt
    71461 tgacaggaac ttttttcgcc acacctgcca cataaaactg ttctttcttt tcgatacgaa
    71521 attccatcga ttttcctcct ctgacatcaa taaaaaatgt caagcgtgga aaagattttt
    71581 gtatgccgta tctgctgatt tctgaaggca gatatcctga ccaatcacga aaagctcttg
    71641 aaaaaccttc gactgtttca tatccatatc taaacgccgt ttctgtcact cttgatccat
    71701 ctaggagatc gcgattagcc aaagccaatc tacggttttt gatatagtcc attagcgaca
    71761 tccctgcaat aaacgtaaag atgcgtttta attgatattc agagattcct gtattttcgg
    71821 ctattatttt ataggaaagt ccttctgtca aatgttcttc gatgtaatcg atcaattggt
    71881 tcagatcttt aagcatattt atcttcctcc ttgcttgcct ttttatcata ttatcaaaga
    71941 aagtcaatta cttgatgaat caggaaccaa tttagtcaga tgtagaaaat gttgtatgga
    72001 tctaaagaaa attctcaagc agcgtttcgt aacggataat tggtattatc tgcctatcta
    72061 tgttttacct ttagaaaaat aaattttggg gaagacacat tctaagtaaa caaagcggta
    72121 aaaatatctt ttattccctt agttaatccg cttactacct actgtacaaa gcaaattgtt
    72181 gttcaaactg ctgatcagaa cgctgcttgg atttttcttt gcgatacatc atacctcttt
    72241 tatcaattgg aacaattatt tatttctaac aaaatcactt catttttgtc agataaataa
    72301 aaaacaaaaa aagttccatc tttaaaacac gctattcttt tactttttct cacattctct
    72361 aagaaaagaa aaggagaatg aatttttgac aattcgagca aaagtgatta tcaaatatgt
    72421 aaaatcagct acttttttct taaacgtttc tttatactaa atataaataa gtgtttcatg
    72481 aaaggagaac tagatgaata attggcttga gtattttcct gaaaacgttt tggaacgcgg
    72541 ttacagctat catctgcacg gttttgtccg tcatttgaac tatacaagca agtacttgtc
    72601 cgcaacagtt tctggcacgg aagattataa ggtagtgatc acttgggatg aaaagaccaa
    72661 catgacttgt gattgtctat atgccataga aggaaaaaaa tgcaaacaca tggctgctgt
    72721 cttattcgct tacgaagaac gtccaataaa aaaatccaac tactcacttt ccgaactctc
    72781 gtcccttgtt tcctccgcta gttcctctct tgtacgggag cttttaacag agattcttat
    72841 agaacatccg caatttatcg aacgcttcaa agttaagatg ccatttcatg caataaatta
    72901 ttcagataaa ttaacaacta tcatccataa atacgatcat attatcaaga aaaacaaaaa
    72961 caggaaaaca gcgaagttca tcatggagat gcgaaagttc atccaagaag cagttgaatc
    73021 gctcattcag caaaatgcct atcttcccgc attcgaattg atcaatgaag tgattgctac
    73081 tctcgaaaca ttctattggg agcctgagga tgaacgaacc cttcttctga tagaggactg
    73141 ttattattta tggaaagaat tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt
    73201 ctcttggttc gtctgccaag tcgatcatac tgacgcctct tattcaaaac gctactccat
    73261 aaaaatcctg aaagaagact ttcgggaaaa agaattttct aaccaaaaga aaaaaataga
    73321 taaaaaaact aaagaaacgg taaagaaaga cgatttcaat gaaaaatagc aagtaaacga
    73381 taacaaccat cctaaatcca caagaaatac agcagacctt gctttaaaaa cacgaatcat
    73441 gcaaaggatg aagtaaagaa gacttaacgc gtacccaata ttagtcattt gactattagg
    73501 tacgcgtttt gttttataag tcatgacaaa tttccttcgt tgctgggtag attttccggc
    73561 attgttgata gattttatga tacttttcta cattttgtaa gagaggttcc actgcatctg
    73621 tatattggat aaaattctga caacaacttt caggtgtgtc gaaccactct aatctttaaa
    73681 gataaatatt aaaataacga ttaataatat taacagtctt tatttttcac caccaaaaac
    73741 ctatctttgc atatactaaa tagacgtata tacacagata ggtttgtctt gtgaaaaata
    73801 atgattatcc ttaccaaaaa atgtttgttt ttctacatgt tcgtacttat caaaaaatat
    73861 gatccttatt gaaattactg gctatttata ttccaacgac ttcttgtgtt aatttttgtt
    73921 atctcatagt aaattatgtt tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc
    73981 gtaaaaacgg taaatatata aacacacttg cggctataat taatatttgt aaaacagctg
    74041 ctcgccagtc tccggctgtt gctaaaaatc ccgatataac tggtggcgta gtccaaggaa
    74101 tttgcactac tacatgatta attaatccca cagcagttgc aaaatatgct actgtcaaag
    74161 aaaaaatcgg tgcaagcaca aagggaataa tcagactgat attaaataca ataggcaatc
    74221 caaaaataat aggttcgctt atattgaata tagcgggagt taccgataat ttagcaaagt
    74281 ccttataatc ttttctacga ctgaagataa atattgcaat caataaagca atcgtatttc
    74341 ccgatccacc cattaccgca aaggtatctt taaaggccat attgataata tgtggaattt
    74401 gctctttgtt tgcataagct gccatatttt cttgcatatt ctgaatcaaa aacggatcta
    74461 acaatgaacc tgatattact gcttgatgga taccaaatcc aaaaaataga ttagcaatag
    74521 atgtaattaa caaaaatcct ggtaaaccag ttgtaatttt acttaatggc tctgtaacta
    74581 tcgtggtaat tagcttattt atatccatat taaacaattg aatgattata aatgacaata
    74641 aagcaaacga ggtaatcgta ataatgattg ggattaatac attaaaagac tttaaaacag
    74701 cgggaggaat atttccggat agctgaatct gtagtttctt attattagaa agtttaataa
    74761 ataattctgt agccgctaat ccaacaaata tacctacaaa cattcctgaa gctcctgtat
    74821 aggatatagg tatcacaccc gtcacttcaa cagctttatc cgcattttca ggagtaaaca
    74881 tcatctgcat aggtgttaat acaacaaatg tcgaaatagc aactaatacc gctgcaatat
    74941 gatttgtata atttctgctt gcacacatat gataagatac tgctgctaca actaataatg
    75001 tagttatgct taatgtacca ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg
    75061 tactagtatc gacaatttcc cccatgaatc ctgttggttc taagatcact gaattaataa
    75121 gtgtcacaaa tcctgctaac atcataaatg gcataaatgt agcaaacgca tctcgcaaag
    75181 ttcttaaatg gatctgattc cccaacttag tcgcaactat accaaatttt tcaaatacaa
    75241 tgtccttcct gctacttttg cccatatttt ttctccttta tgcttattgt aatcgattac
    75301 tttttccacc caaataatac atcacttatc aaaaaaagta aatactttta attaaatata
    75361 tatatctttt ttagtataaa aaaataagct ctgaaaaaat aattttcccg agcttatttt
    75421 tcaacaaaaa cagcttcaaa aacaaagtct tgatataaat gtctcgatac cgaatactcg
    75481 aaaggtttac cagacgatag agcaacaact ttgtctatct caatcataaa ttcacctgga
    75541 tctattttta atatttttgc gtcaaattca ttcgcagtat ctcctttgat acgtatgtgc
    75601 gaagtcttta tttccagacc cagttcattt gtgatataag catatacaga tttctttaaa
    75661 tgttttggtt ccaagcctgg tatgactgct aatggcatga atgtttgctc aatcgagtat
    75721 ggttgacaat caataaaacg gcttcgtaca atattatata catagtcttt tacactgata
    75781 cttaatttat cagctacttt tttcgatgga agttcaattg agaaagtatg gatttctgaa
    75841 tctacgtgtt cttcaccaac cacgctaaac aaaccttcgt ttggaccaag aactcgtgag
    75901 ttatcaattg gtgttctgac gacttcagtc ccatacccac ttctacttcg caaaactcct
    75961 tcggaaacaa gtaagtctac gcctttcttt actgttaact tgcttgtatg ataaataact
    76021 gccaattcat ctcctgtagg aagtttctca ccaattttat actctccatt tacaattttt
    76081 ttctttatat ctaagtaaat ttccatatac ttcgcaattt ttttcaatgg tgaccctcca
    76141 atcaaaaaca tatcttcatc tatactttaa cataaataaa ccgataaaat ccacatcaaa
    76201 attatacaca caataaaaaa gatatatata tttaaacaaa actatttact ttaagttgta
    76261 agcggtgtta tcattaagtg gaaacaggag gtaaaaaaat tatgacaact atgaaaaagc
    76321 taacttttcc agatgatttt tggtggggtt ccgcttggtc agctgagcaa gcagaaggaa
    76381 aaggagaaac aggaaaagca gagactatct gggatcgctg gtttaaagaa caaccttacc
    76441 ggttttatca acgaatcgat tcctctatta caaccgatca catccatcaa tataaagaag
    76501 atatagaact aatgaaaaaa actgggcata actcctttcg cgtttctatt tcttgggcac
    76561 gaatgtttcc aaatggcggt accggaaaag taaatcccaa agccatttct ttctatcgaa
    76621 aattattttc cgaaatgaat aataacggaa tcaaagtatt tgccaactta tatcattttg
    76681 acatgcctgc tactcttcaa gataaaggag gatgggaatc acgtgaagta gttgatgctt
    76741 atgttcatta cgctgaaact tgttttaaag aatttggtga cttagtttgt cattggttta
    76801 ccttcaatga accacttggg cctattttag gtacttatct tgaagatttt cattatccaa
    76861 acctcattga ctttaaacgc ggagcgcaag cagcattctt cactatactt gcacacgcaa
    76921 aagcaataca agtatttaaa gaactagctc ttcctagtaa aataggcgtt attctaaatt
    76981 tgagtcctac ttttcctcgt agtcaaaatc ctgctgatgt gaaagcagca gaaatcgctg
    77041 atttatttta tacgcggagc tttttagatc ctatggttaa agggaatttt tctaaaaagc
    77101 ttatcctgtt attacaagaa tataatcagc ttcctgaaga ttatacgaaa gaagatcttg
    77161 tatgtatcta cgaaaatacc gctcaaattc ttggactaaa ctattacgag ccacgtcgcg
    77221 tcaaatctcg ttcttctatg gtggatacat ctagtccatt tttaccagag tggtttttcg
    77281 aaccacatat catgcctgga cgacgaatga acgaataccg gggatgggaa atttacgaaa
    77341 aaggaattta tgatttgtgc atggatatca agaaccatta tggaaacatc gaagcattta
    77401 tctcggagaa cggaatgggc gttgcggatg aagaacgttt catggatgat acagggcaaa
    77461 taattgatga ttatcggatt gagtatatca aagatcatct tgcttactta tggaaggcaa
    77521 taaatgaagg atgtaacatt aaggggtatc atctttggac atttattgat tgctggtcat
    77581 ggatcaacgc ctataaaaac cgatatgggc tggtttcact cgaccttact actcaaaaaa
    77641 gaacaattaa aaaaagtggc gaattttaca agcaattaag tgatacaaat ggatttagat
    77701 acgatgtaga cttgttagtt gaataatatt ctaaaattcc acttgaagtg aacacattag
    77761 aaaacaagaa taatgagaaa taccaataac aaatactttt tcttcatact tcatttatac
    77821 ttgtcagtat aagagactgt gacaaatgta gtgatcctca gaagttaggt ctggaaatct
    77881 aacttgagaa gattttttta aactacgtcc agtctttttt atttgtttaa aattgaagtt
    77941 gacaagtctt cattaaaaat ttagaatatt cacaagtttg acgacaatta tgaaaattta
    78001 tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac tgcgatcaaa
    78061 gacacgttac ctaccgtttt tggttatatc ggtattggac ttgcatttgg tatcgttggg
    78121 aaagctgccg ggtttcatcc gctggtcgtc acgttgatgt ccctgctgat ctatgctggt
    78181 tctgcccaat ttatcacggt cagcatgctt gctagccata gtccactgct ctccatcgtt
    78241 ctctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat tgctccttat
    78301 ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac agatgaaagt
    78361 ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt tcgttggatg
    78421 aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt tggcgcactt
    78481 ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat cgtcgctatg
    78541 tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa acgattacag
    78601 ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt tatcccaagt
    78661 aacttagtga ttttagttgt aacactgatt gggtgcggat tgggggtgtg gatcaaacat
    78721 gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa cttggctttc
    78781 taggatccta ccatttgtct tactgaaaaa attcgatttg ccgcaaccgt tgcttgagta
    78841 tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc tatttaccca
    78901 aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag ttcctacact
    78961 tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggtaggaa ttctttccct
    79021 ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt actgtcatgc
    79081 gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc ttatccgttg
    79141 aacggaccac taacaacgac ttttcctaat atatgtcctg tttctacgtc tttggttgct
    79201 tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt actgcggata
    79261 atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca
    79321 tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga ttggtttttc
    79381 caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg tccaaatggt
    79441 cggacaaggt gtttgatttg atcaaagtag cgggtaatat cgaataatac agcgacatac
    79501 tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg ataatcgatt
    79561 ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc agtcgcgtag
    79621 acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc cccagctccg
    79681 ttgatcacta agattttttt cccgcggttg gcattttctt ctggagtcag accaaatttt
    79741 tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcggc ttcttcatct
    79801 gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ccagttggta ttcttgattg
    79861 cttcctgctc ttgtcgttgt acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc
    79921 acttcatcac ctacagcaac aactttcccg actccatcaa accctaagat acgtaaagca
    79981 tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga tactgcgttg
    80041 acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc atcaataaag
    80101 ctttttggat catcgatcga caacccttca taaaaaccga ctaccttcat taattccttt
    80161 tctgacatac gaattcctcc ttagcttcat caagttggag catccataca gttttcattt
    80221 caatatatat agtatgctat ttgttccact tttattcatc ataattattc tcttacttaa
    80281 agtaaactat ttgcactttt ttggttaaaa aagaggttgc gacaagactt ttgtcacaac
    80341 ctcttttaaa cggtgttcaa tcaactgacc ctcggtattc gctcaaaaaa cgacaaaata
    80401 tgaggagcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacgac
    80461 ctctaattca cggtgttcaa gcaagaaatc attgttcact gaaaagcgtt ggatgttctt
    80521 tttcaaaggt gtctaagatc tctcgtactt catcagtaga tttcgtattc atcaactgaa
    80581 ctcgcaagtc atttgctcct ggaaatcctt tgatatagat tttaaagaaa cgatgtagtc
    80641 ctgtgatgga acgcgggatg atttctgaat aatgatcttg cagatctagt tgcagacgaa
    80701 gtagtccgat cagttctttt tctgtatgtt ccctcggttc tttttcaaat gcataaggat
    80761 tcttgaaaat cccccggcca atcatgatgc catctacgcc atatttttcg gccaattctt
    80821 ctcctgtctg gcgatctggg atatctccgt tgatcgtgat cagtgtttgc ggagcgattt
    80881 ggtcacgtaa agcaatgatt ttagggatga cttcccaatg tgcatctact ttactcatct
    80941 ctttacgcgt acgcaaatgg acagaaagat tggctatatc ttgatgaagt aggtgactga
    81001 tccattcttc catctcagac atttccgtat agccgatacg cgtcttgacg ctgacaggca
    81061 aaccgccagc tttagccgct tcaatcaatt gtgccgctac ttctgggcga aggatcaagc
    81121 cactgccttt tccacgttct gctacattag ggactgggca gcccatgttg atatcgattc
    81181 cttggaagcc catttctgcc atagcaatgc tcatttctct aaaaaattct ggcttgtctc
    81241 cccaaatatg ggcaaccatc ggctgttcat cttctgtaaa ggtcaatctt cctcgtacac
    81301 tgtcttttcc atctggatga cagaaactgt ctgagtttgt aaactcagta aagaagacat
    81361 ccggtgctgc tgcatgcttt acgacatggc gaaatacaac atctgttaca tcttccattg
    81421 gtgctaaaac aaaaaaaggc tttggcagct ctttccaaaa attagtagtc atttatttgt
    81481 tactctccat tctatcaagc agtgaagtcg caatctttct caaaaacatt gtcattatac
    81541 taggaaacaa aaaaagaagc aaagtgaaca gcttaaaaga tgtttctgat tgagcaaaaa
    81601 aagactgtga caaaaatctt atcacaattc cttttccgga ataaatggtg ttcaaaagct
    81661 tgcatctgcg gtaataagtc caaccaaacg aaaaatagga ggagctattt ttgtttggct
    81721 gttcttatta cttgatgcag aacagctttt tcacaacctc tccactaaac ggtgttcaat
    81781 cagctgaccc tcggtattag ctcaacaaac gacaaaatat gaggaactat tttcccgttt
    81841 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttttctaa cttctcaatc
    81901 ctcttccttt attgatcaaa taccaagtaa cggcgtacaa aacaacgatg aatagcacaa
    81961 gtacgatcat cgatgttgct actggcacat cgattgttcc aaggaatccg taacggaaac
    82021 cgaagatcat ataaacgatc ggattgactt tcgaaacagc ttgccaaaat ggcggcaaca
    82081 tagagatggc atagaaaacg ccacctagat aagttaacgg ctgcaagaca aatgtaggca
    82141 cgatcgatac atcgtcataa gactgagcaa aaatcccgtt gagcagtcct gctaacgaaa
    82201 acaagattgc cgtcatcaat aatgtaatga tgaccatgaa ccatgaatac acatgaagtg
    82261 gtacaaagaa caaggaaatg atcgttacga gagagccgac gagaatactg cgacccactc
    82321 cgccaatcac aaagccccaa atgatcacat gcgtaggaac aggcgctacc aagatttctt
    82381 cgatgttttt ctggaatttt tgagagaaga aggacgaaga aacgttggca tacgaactag
    82441 tgatagcaga catcataatc aaaccaggca cgatgaattc catatatgaa aaaccaccca
    82501 tgtcaccgat ccgaccaccg atcatttttc cgaaaataac aaagtacaag gatgtcgtga
    82561 tcacaggcgg aaccagtgtt tggacccaga tacgcaaata gcggttcgtt tccttgatag
    82621 ctaaactttt taatgcagta aaataaagat taaacatttt tctcctccgt atgattattt
    82681 tcttctgtga ttttcaaaaa cagttcttct aaacgattcg acttattcct catagaaaga
    82741 actttgattc cttgtcttgt cagctgatcg aagattccgt tcaccccttg atttcgttca
    82801 acttcgacag cgattgtctg ttcatcttca aatgtacaag cgtatccttc aatcacaggt
    82861 ttggtctcat atggcgctaa atcaaagata aatgtttcaa attgcagttt ggatagaagc
    82921 tctttcatac tagtattctc gatcagttca cctgattgga tgatcccgat gttgcgacaa
    82981 agcatttcag cttcttctaa ataatgcgtc gtcaaaatga tcgttgtacc attttcgttc
    83041 aattctcgga gaaactccca catctcgcgt ctcaattcaa tatcgactcc tgctgttggt
    83101 tcatccaaga tcagcaaacg tggttcatgc atcaatgccc tagcaatcat cagtcttcgc
    83161 ttcattcccc cggaaagcat acgtgcacgg acatttctct tttcccaaag attggactgc
    83221 ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag agacaccgta gtagcctgct
    83281 tgattcacga cgatttgctg gaccgtttca aagggattga aattgaactc ttgcgggact
    83341 aatccaatct gttgctttgc tcggactaga tccgtatcca gatcataacc gaagacattc
    83401 acttttccag atgttttatt gactaaagaa gtaatgattc cgatcgttgt agacttccca
    83461 gctccatttg gtcctaatag cgcatagaaa tctccttctt tcacggaaag atcgattcct
    83521 cgcaatgctt cgaccccact tccataaacc tttttcaaat tatttatttc aagcgcattt
    83581 gccatttatt tcctatctcc ttatagacat gattctttat tgtatcatgg aatacagcag
    83641 atgaaaaaac aacttggttc cagcgaaaaa tcaggttgct gggctcagga aaatctccta
    83701 atgaaagaca tcaatcggac gaagtcagac aaccttttca gaaaaaacaa atcgaagata
    83761 aaggattact attcatatgt gcaacaaaag gagcgtgaga caaaagtaaa gaacactttt
    83821 gtctcacgct ctaaacacgt ataaacggcg gaagcaaaag ccaagatttt tgatccaata
    83881 aatactttcg atcacttcgc ctatcccaat acatgccact gccgcttttc atcaaataac
    83941 gcggcttccg tttttgcggt ttctactaaa gtctctgggt aatccaaaac tttcagcaag
    84001 gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt tgaaatcaaa actgattcct
    84061 tttccttctg ttacttgttc ttcaaagtgg acattctcac aataattctt taaaatctct
    84121 gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg gatactccgc aagccaccga
    84181 acgactgaag cggaagaagc aatccgttcg atcgtatttg tcccttttaa aatctcatct
    84241 acgaagcagt agcagcgttc tttggaagcc acttggtcca acaaacgttt gatcgatttg
    84301 atttctgaaa caaaataact gtcgccttca aataaatcat cttcgattgc catggaagtc
    84361 aacacatgtc caggttgcag ggtaaactgt tctgcaatcg ctgtttggat cgtctgtgct
    84421 agaatacagc tgatggctat acttttgaca tacgtagatt ttcctgatgc attggaaccc
    84481 gtgactaatg tattcttctg ccagttgacc ggattcacta ccgcttcttt taacaatgga
    84541 tgatagatat ctttcgctgt cacaccgccc tcagtaaact ctggtcggca agtgatgggc
    84601 atataagtac ggtaattcaa tattgcagcg gctacttcta attctcccag caattcccac
    84661 agcttgatgg cttcagttga atgcttttct aatttagtga gaacgaaatg ataggagata
    84721 aatggcagca tgaacatgat gttgacatat tcaaaaatca actctgcttc actcccactc
    84781 ttcatacgaa atgaaaaacc taatttaggg atgtctctta atggtttagt ttgctgtttc
    84841 agctcttctt gaagtggtgt cggcagtttt gctagctgat tggcggctga aatcgttgaa
    84901 accaaataac tcatgctatt cagctctgtt tctagtgttt gtttcttcac tagataatag
    84961 accgtattga atactaaccc accgattgcc aaatagattc ctagtacttg tcctaaaaga
    85021 agcaaaccca aacccaccag cggaagcaag ccaagaaaaa taaataaacc tacgcgtcca
    85081 atctttttac tttttccatc cgctagatat tgttttgcaa aattatgatc tttttttcct
    85141 aaacgagcga attgatattg gattttttct ctcagttgag gattttcctg ataaaaagca
    85201 atcagttttt ctaactgctg atcttcacca aaatcaaagc tgcgtaaacg ttgatacaac
    85261 gcttctgaac ctacactaga ataggtactg ttgattcctt cgaatacttc gaacatatcc
    85321 aggtcatacc aagtaagatc atcgatttcg ctatcccatg aacggaactt cttctccgtg
    85381 agccatgctt ccttcaagct ttcttcttta tcaaatctcg gttgataagg aattttttcc
    85441 catttgctac gaactgtttc ctttaatttc aaacgtgaaa aaatgtctaa agcaactaat
    85501 aaaataacag ctgcaataat aattaaaacg ataaattctc tttgacccat atccctctcc
    85561 gttcctttat ttgttagctt tagtatagca ctgttgcgct atttgaaaag agggtaaaaa
    85621 aacacctttc aaaataaaag gtgttttttt cttgtatatt acatatttat ctctcattta
    85681 tcaaaattga caaataatca tagggaggaa ttgttgttaa ttcttgttta ttctccagtg
    85741 cattgacaac tgtcccaagg gaaaggatag cctcaggtac tattttttct tgttctgttg
    85801 gattagctaa gcgaagaaca aatccttgtt catgaaaata agaaggataa atcgatgata
    85861 caattaagtc ttcaggtaat tcaatcaatg aaaatttacg aggaacatca agttcatttt
    85921 gtaatggcca aatcttgtta tctaacctgt gaataaatac attcagtttc tgcttttgat
    85981 aagaaataga ttgttctaac cgtttttctg cctgttttgc cactttgaac tcattaaatc
    86041 ttccttcttc tacattaaat ccaaaagtga ctttatactc acctatctct tgagctagcg
    86101 gtgtttccat catgacatgc ccttcatttg tcgtatctcc tgaagctcgc ccaggtcgcc
    86161 aagcaagatt aggttttcca agttcccctg ttgttgaaaa taaggttatg attaattgat
    86221 tttctatacg ttcatactct tttaaaccat ctgcataaaa cgtaaaataa tgatcatttt
    86281 ctgccacaga gacactctta tcgaatattt ctaaattaac aggtttctct accatagttt
    86341 ctgaccaatt cggaggaatg acatcagggg tattttcaat aaatccgttt tgaatttgtg
    86401 caattgattt ttcagattta tctaaaacat taattgctaa gcgcatacga tgcgagtaaa
    86461 cagtattatg aattttgagt gtgccttcaa cgatttgact ttctcccaga ctgaacgata
    86521 tttcataaga tatttcttcc atttcgggtt tttcagataa acgatctttt aaatctttag
    86581 gaagttgagc ttttccgtaa acaactaatg tttcttgtaa agaatcttta tatactttag
    86641 ctgtttctaa ttttagagat agttctgtat ctccttctaa tggtgaataa tcatacgtat
    86701 cgccatcatt tgctgaatcg atcaaatgaa cgaaatcaag atattgtcta tctccaacta
    86761 tcaaattcaa ttgcccatct ttaaaagaaa gtttatattt attgttactt atctcagttt
    86821 tgtctatctc gattgctgtt tctaattttt cattattttt ctcaaattca acaacagtgt
    86881 atcctagtgc tggcacattt acatttattt ttatgtcaag ttcataataa gctggttcat
    86941 caaaatattc gaaacctgta gcaatcatcc gataagcatt tttccgttct ggaaaataaa
    87001 cttctttttc aatgatatta tagggactac ctttgaagtg tattttcttt gaatcagcta
    87061 ctacatgcac gattttcgat ccactataat tctcagtcgt tgtgttgaaa acaaggactt
    87121 cattcgattt tagctttaat gcatcagcca tccgttttac aataaggttt tcgattccgt
    87181 cagctatctc attagcttct ttgattcgat ggaaaatatc ttccgcaaca ttatctgaaa
    87241 cacagccacc aatactatca tgagcctggt tttgcaaaat ctttttccat aaccgacgaa
    87301 gtaggccttt actgatatgg ataccatttc tttctgcaat cagcatgagc ggctcaactc
    87361 gttttagaat tttttgttcc aatttaaaat tttctatttt catttttgtt cttactgagc
    87421 caatagaacg atgaacacga gagtaaaccg gctgacgaag ttcaccttta tattcaggta
    87481 attctttttt gtttctgata acttctacat aagaaggata atctgaaata atattgtgga
    87541 atttactgta tttattcaac ttctctacga cttgatcaaa atttaaaatc attgactttt
    87601 gatctacacc cgaaggtact aaaatatctt tgttttttcc ttgcatatga agcatttcaa
    87661 ctgcttcatc caacttttct tttgcaaatt caggaatatc tttaattgcg tctaaggtca
    87721 tcattccagt catataacca aatggaaaat tcatggcata aacaaattta tctcccaatc
    87781 ctttccagat aaaataagga gatggaacca tcgtttcaaa gtttaatcct cgccaacaca
    87841 tgaaattatc gatttctact tgatgcaaaa gtgtaggcaa attcgcattg aacccaaacg
    87901 tgtcaggcaa ataccctacc atcattggat ctccatattt aaggatcgta tcactgattc
    87961 caatcattaa gtttcttaaa attgattcag catccactaa taaggcatct gtttgtgcat
    88021 accacggtcc cacgaatagt tgtcctgttt tcatcagtcg ttggataacc ttaagctttt
    88081 gcggattgat ctccaaatac tcatcgacga ttgaagtttg tccatctaaa caaaagttta
    88141 ctttagggtt cctttccaat tcatctagaa cttcagtaaa gacctgatca gacaatacga
    88201 tggcatcttc atttgtaaaa taccattctc tgtcccagtg cgtgtgttga attatatgtg
    88261 catttaccat atcagattct cctactttcc ttcttctact gaatttactt gttgttcttt
    88321 tttatacttg atggatttta gtgcattgac cattaacgct gtaaccactc ccccaataac
    88381 aattgatact aaatataaag gtatattcgt aacaaaagga attgccataa aggttgaaag
    88441 cattgtttct tgagtaacac caaaaaatcc atttagacca catgctactg cagtgcccac
    88501 cacgatgctt ggtattactc gtaaaggatc tttcacggca aacgggatcg tcccttcagt
    88561 aatccccaca aggcccataa tcatagctgt ctttgcatta tctcgttccg attcatcata
    88621 aagttttttg tttaaaattg ttgctaaaaa cattccaaat ggcggaatag caattcctaa
    88681 taacatggct gtactaggtc caaagcttcc gcttgaaaaa gaagcaaccc caaaagcata
    88741 tgcgatttta ttgataggtc ctcccatatc aaacgccatc attcctccta aaatagccga
    88801 catcaaagca gcatttgctc ctgacatgcc ttctagccaa ttagtcagtc ctgttgtcat
    88861 tgcttgaata ggcgtagcta gtacatacca taatatgatt ccaacaacta atgtagtaac
    88921 aaaaggaatc agcataattg attttagact aataacttgc atcggtactt ttactttttt
    88981 caatgcatta gctaaatagc cacaagctaa tccagcaacc attcctccaa taaatcccat
    89041 accaagattt tgacacatca tcccagtgat aaaagctggt gctagaccgg gacggtcagc
    89101 aattgaatat gcgatatagg ctgaaatcat cggtacgata aactgtaatc ccgtctgacc
    89161 aatcattcgc aggactcccc agaatgtatc cgtctggtcc caaacacctg tgccaccaaa
    89221 aatgactgca atagaagtta gtaaccctgc tgctacgaca agcgggatca tataggaaac
    89281 cccactttgg aaatgattta gaatttctct tccttgtttc ttgaattcca ttagtaactc
    89341 ccccacgata tattttttat ttgttaatta atgcaattgc ttcatcaatc gtctcttcac
    89401 catcttttac tacctctgag gtccctactg ttaaaatcgg tttaccgtca aagcgacctc
    89461 gttctgaaac atttgtatct actgcaaata tgacaacatc tgcctcggaa acatccgaaa
    89521 ttgaaagttt gttttctatc cccatagccc cctgtgtttc tactttaata tctaaccctt
    89581 tcttcttcgc aaacttttct aaattagctt ttgccatata agtatgggca atccctgcga
    89641 tacatgctgt tactgctact atttttgtca tatgaaattc ctccttttaa aatgatagag
    89701 cttttaccaa ttctttttta tttctcgaat cttttattct tttaacaaac tcgtcatcca
    89761 tcaatcttcc agataattct gctaaaatac gtaaatgatt agtaccttta tcagtgtctt
    89821 ttattgctaa caaaaagacg agatcaactg gttgatcatc taatgcgccc cattcaacag
    89881 accgctttaa cttggctaca gccactgttg attcaattac tgctaggctt ttgccatgag
    89941 gaatggcgat atttttccca attcctgtgg tcatatggtt ttctctgtca aatacgtctt
    90001 tttgaaattg tttgtggtca gacacaaacc cattagtctc taacaatttc gataattttt
    90061 cgactacact ttttttatca gatgcattta aatctatcac tatattattt atattgattt
    90121 tttcactgat catttataag ccttctttca atatataaac taatttttca aacgacgaac
    90181 tcgtttttaa atttgctaat aactcatgat tcgaagcgat ttcattgaat gtttcataaa
    90241 ttttattcaa ttcaggtttt actttttcat taagtgcaaa aaagaaaaca gaatgaacaa
    90301 gtgtcccatc ccaatcaatt ccttctggat taacgtagac acagattact ggctttttta
    90361 taaaatctac ttcgatatgc ggcatagcaa aatcattcat cgctgttgaa gatagttttt
    90421 ctcttttgat tgctccctct agtatccctt ctttcgcgta attttctttt accaattttt
    90481 tggtaattat atttaaaacg ttcacttgat tatcctttat ttggtgggta aaaaagcaaa
    90541 atcgtttatc taataaattt agaaaagcat cttctgtttg ttcatttaac tgtagatact
    90601 gttttacttt tctgacatct gcccctaaca tcaaaggact aacaacaata acaggaaaat
    90661 ttgtattttc aatcggaatc gtgcttataa caagttcatc agtgactttt ctatcttgta
    90721 gttcattgat gcttagtaca tcttctatct caaccatatc tgcaaaaccg ttatttattc
    90781 tttgctctaa taattttgat gttccatatc cgctactaca aactaatatc actttcgttt
    90841 tgtcaggttt gtaacgatcc aaaagtgatt gaatatggag tgttacgtaa gcgatttcat
    90901 cttcattaaa atgtatacag aatctctctt cgatttcctc taataaatct acacttatca
    90961 taaatgacct tttaaaagaa tatttgattt tgtccgtata agggttatag atatttactc
    91021 ccaatttgag tcttttaact gctgcattga gatgtaatac tagattattg atcaattctt
    91081 catcaggata aaattcattt aatttaattc ttactaactc ttgtagctca acacccatat
    91141 ctaaatagga aagcttcgtt tgatctaagt cgtgattcat agcatttttt tcaatcgaag
    91201 taatgtgaat gtcaacataa gcttgttcat ctattggcac aataattgaa aatgcttttt
    91261 caatcatttt gattaaaata agtgtattct ttgagatttc ttcttcagaa ttattactac
    91321 gtgataagaa attgcttgtt tcaatccttt ctatcgcaat agcaaggtgg atagctaaag
    91381 tttgaaattc ataatctgta aatttcaatt gtgtcttatt catgtatcga ttaagcaatt
    91441 cggctacttt tttcaatatg tccttattta agaaattatt cgcttcacca atgaaattga
    91501 tattcaagtc taagagatct gttttactgt tagatgctgt aaagccaccc caataatagt
    91561 tcacaacttc tgacatcaac cttcttttac ttttttcatc agctttgatg cgcacgccat
    91621 ttcgatctga aactaaggac atcccatgct tgtttagtct ttttctaaca tctttcattg
    91681 tgctttctac cgtcgacctg ctcacgtata attcgtcagc aagattttga atctttagag
    91741 aatcattagt attcaataat ttagcaaaca cataagttag acggtcctcc ggagagtctg
    91801 cgatattgat tttatgactg gataataaat ttcgaagtgt ttcttttttt ccaataagcg
    91861 aaacgccttt tttaggttga accaacattt gtacatctgg cattttttgg attatttctt
    91921 tcaaaattaa aatatctttg ctaattgttt tcgtagatac atataat
//
LOCUS       DACEDD010000003        90181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEDD010000003 DACEDD010000000
VERSION     DACEDD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.003"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            315..3149
                     /locus_tag="IWO45_000178"
     CDS             315..3149
                     /locus_tag="IWO45_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0331688.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3341..3832
                     /locus_tag="IWO45_000179"
     CDS             3341..3832
                     /locus_tag="IWO45_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0331689.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3935..4924
                     /gene="manX"
                     /locus_tag="IWO45_000180"
     CDS             3935..4924
                     /gene="manX"
                     /locus_tag="IWO45_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0331690.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4957..5760
                     /locus_tag="IWO45_000181"
     CDS             4957..5760
                     /locus_tag="IWO45_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0331691.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5781..6695
                     /locus_tag="IWO45_000182"
     CDS             5781..6695
                     /locus_tag="IWO45_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0331692.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6778..7152
                     /locus_tag="IWO45_000183"
     CDS             6778..7152
                     /locus_tag="IWO45_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0331693.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7291..7536
                     /locus_tag="IWO45_000184"
     CDS             7291..7536
                     /locus_tag="IWO45_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0331694.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7708..8772
                     /locus_tag="IWO45_000185"
     CDS             7708..8772
                     /locus_tag="IWO45_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0331695.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9018..9443
                     /locus_tag="IWO45_000186"
     CDS             9018..9443
                     /locus_tag="IWO45_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0331696.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9436..9885
                     /gene="nusB"
                     /locus_tag="IWO45_000187"
     CDS             9436..9885
                     /gene="nusB"
                     /locus_tag="IWO45_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0331697.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9982..10836
                     /locus_tag="IWO45_000188"
     CDS             9982..10836
                     /locus_tag="IWO45_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0331698.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10837..12174
                     /locus_tag="IWO45_000189"
     CDS             10837..12174
                     /locus_tag="IWO45_000189"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0331699.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12179..12403
                     /locus_tag="IWO45_000190"
     CDS             12179..12403
                     /locus_tag="IWO45_000190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0331700.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12405..13295
                     /locus_tag="IWO45_000191"
     CDS             12405..13295
                     /locus_tag="IWO45_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0331701.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13304..14122
                     /locus_tag="IWO45_000192"
     CDS             13304..14122
                     /locus_tag="IWO45_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0331702.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14229..14678
                     /locus_tag="IWO45_000193"
     CDS             14229..14678
                     /locus_tag="IWO45_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0331703.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14696..16378
                     /gene="recN"
                     /locus_tag="IWO45_000194"
     CDS             14696..16378
                     /gene="recN"
                     /locus_tag="IWO45_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0331704.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16433..16549)
                     /locus_tag="IWO45_000195"
     CDS             complement(16433..16549)
                     /locus_tag="IWO45_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0331705.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16622..17578)
                     /locus_tag="IWO45_000196"
     CDS             complement(16622..17578)
                     /locus_tag="IWO45_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0331706.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17749..18117
                     /locus_tag="IWO45_000197"
     CDS             17749..18117
                     /locus_tag="IWO45_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0331707.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18287..18718
                     /gene="mraZ"
                     /locus_tag="IWO45_000198"
     CDS             18287..18718
                     /gene="mraZ"
                     /locus_tag="IWO45_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0331708.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18739..19698
                     /gene="rsmH"
                     /locus_tag="IWO45_000199"
     CDS             18739..19698
                     /gene="rsmH"
                     /locus_tag="IWO45_000199"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0331709.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19703..20104
                     /gene="ftsL"
                     /locus_tag="IWO45_000200"
     CDS             19703..20104
                     /gene="ftsL"
                     /locus_tag="IWO45_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0331710.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20105..22291
                     /locus_tag="IWO45_000201"
     CDS             20105..22291
                     /locus_tag="IWO45_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0331711.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22317..23279
                     /locus_tag="IWO45_000202"
     CDS             22317..23279
                     /locus_tag="IWO45_000202"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0331712.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23293..24654
                     /locus_tag="IWO45_000203"
     CDS             23293..24654
                     /locus_tag="IWO45_000203"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0331713.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24680..25768
                     /gene="murG"
                     /locus_tag="IWO45_000204"
     CDS             24680..25768
                     /gene="murG"
                     /locus_tag="IWO45_000204"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0331714.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25783..27003
                     /locus_tag="IWO45_000205"
     CDS             25783..27003
                     /locus_tag="IWO45_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0331715.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27159..28484
                     /gene="ftsA"
                     /locus_tag="IWO45_000206"
     CDS             27159..28484
                     /gene="ftsA"
                     /locus_tag="IWO45_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0331716.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28507..29748
                     /gene="ftsZ"
                     /locus_tag="IWO45_000207"
     CDS             28507..29748
                     /gene="ftsZ"
                     /locus_tag="IWO45_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0331717.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29765..30442
                     /locus_tag="IWO45_000208"
     CDS             29765..30442
                     /locus_tag="IWO45_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0331718.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30456..31055
                     /locus_tag="IWO45_000209"
     CDS             30456..31055
                     /locus_tag="IWO45_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0331719.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31070..31357
                     /locus_tag="IWO45_000210"
     CDS             31070..31357
                     /locus_tag="IWO45_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0331720.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31406..32188
                     /locus_tag="IWO45_000211"
     CDS             31406..32188
                     /locus_tag="IWO45_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0331721.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32209..32916
                     /locus_tag="IWO45_000212"
     CDS             32209..32916
                     /locus_tag="IWO45_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0331722.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33178..35976
                     /gene="ileS"
                     /locus_tag="IWO45_000213"
     CDS             33178..35976
                     /gene="ileS"
                     /locus_tag="IWO45_000213"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0331723.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36025..37551)
                     /locus_tag="IWO45_000214"
     CDS             complement(36025..37551)
                     /locus_tag="IWO45_000214"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0331724.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37566..38213)
                     /locus_tag="IWO45_000215"
     CDS             complement(37566..38213)
                     /locus_tag="IWO45_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0331725.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38397..38726)
                     /locus_tag="IWO45_000216"
     CDS             complement(38397..38726)
                     /locus_tag="IWO45_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0331726.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38903..39631
                     /gene="sfsA"
                     /locus_tag="IWO45_000217"
     CDS             38903..39631
                     /gene="sfsA"
                     /locus_tag="IWO45_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0331727.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39647..40660
                     /locus_tag="IWO45_000218"
     CDS             39647..40660
                     /locus_tag="IWO45_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0331728.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40660..41937
                     /locus_tag="IWO45_000219"
     CDS             40660..41937
                     /locus_tag="IWO45_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0331729.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42000..44702
                     /locus_tag="IWO45_000220"
     CDS             42000..44702
                     /locus_tag="IWO45_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0331730.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44854..45171
                     /locus_tag="IWO45_000221"
     CDS             44854..45171
                     /locus_tag="IWO45_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0331731.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45201..45521
                     /locus_tag="IWO45_000222"
     CDS             45201..45521
                     /locus_tag="IWO45_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0331732.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45629..47089
                     /locus_tag="IWO45_000223"
     CDS             45629..47089
                     /locus_tag="IWO45_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0331733.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47157..47378
                     /locus_tag="IWO45_000224"
     CDS             47157..47378
                     /locus_tag="IWO45_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331734.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47409..47591
                     /locus_tag="IWO45_000225"
     CDS             47409..47591
                     /locus_tag="IWO45_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0331735.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47591..48004
                     /locus_tag="IWO45_000226"
     CDS             47591..48004
                     /locus_tag="IWO45_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0331736.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48127..49308
                     /locus_tag="IWO45_000227"
     CDS             48127..49308
                     /locus_tag="IWO45_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0331737.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49697..49770
                     /locus_tag="IWO45_000228"
     tRNA            49697..49770
                     /locus_tag="IWO45_000228"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49731..49733,aa:Arg,seq:cct)
     gene            complement(49807..50007)
                     /locus_tag="IWO45_000229"
     CDS             complement(49807..50007)
                     /locus_tag="IWO45_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0331738.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(50312..51544)
                     /locus_tag="IWO45_000230"
     CDS             complement(50312..51544)
                     /locus_tag="IWO45_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0331739.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            51798..52367
                     /locus_tag="IWO45_000231"
     CDS             51798..52367
                     /locus_tag="IWO45_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0331740.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(52545..52985)
                     /locus_tag="IWO45_000232"
     CDS             complement(52545..52985)
                     /locus_tag="IWO45_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0331741.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            53143..53907
                     /gene="map"
                     /locus_tag="IWO45_000233"
     CDS             53143..53907
                     /gene="map"
                     /locus_tag="IWO45_000233"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0331742.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            53939..54862
                     /locus_tag="IWO45_000234"
     CDS             53939..54862
                     /locus_tag="IWO45_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0331743.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            54938..56077
                     /locus_tag="IWO45_000235"
     CDS             54938..56077
                     /locus_tag="IWO45_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0331744.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            56070..56870
                     /locus_tag="IWO45_000236"
     CDS             56070..56870
                     /locus_tag="IWO45_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0331745.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            56870..57697
                     /locus_tag="IWO45_000237"
     CDS             56870..57697
                     /locus_tag="IWO45_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0331746.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            57675..58409
                     /locus_tag="IWO45_000238"
     CDS             57675..58409
                     /locus_tag="IWO45_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0331747.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            58509..59375
                     /gene="rfbA"
                     /locus_tag="IWO45_000239"
     CDS             58509..59375
                     /gene="rfbA"
                     /locus_tag="IWO45_000239"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0331748.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            59389..59961
                     /gene="rfbC"
                     /locus_tag="IWO45_000240"
     CDS             59389..59961
                     /gene="rfbC"
                     /locus_tag="IWO45_000240"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0331749.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            59983..61011
                     /gene="rfbB"
                     /locus_tag="IWO45_000241"
     CDS             59983..61011
                     /gene="rfbB"
                     /locus_tag="IWO45_000241"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0331750.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            61109..61960
                     /gene="rfbD"
                     /locus_tag="IWO45_000242"
     CDS             61109..61960
                     /gene="rfbD"
                     /locus_tag="IWO45_000242"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0331751.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            61995..64028
                     /locus_tag="IWO45_000243"
     CDS             61995..64028
                     /locus_tag="IWO45_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331752.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQGNQLVVQ"
     gene            64072..65352
                     /locus_tag="IWO45_000244"
     CDS             64072..65352
                     /locus_tag="IWO45_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0331753.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            65562..66368
                     /locus_tag="IWO45_000245"
     CDS             65562..66368
                     /locus_tag="IWO45_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0331754.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            66380..67600
                     /locus_tag="IWO45_000246"
     CDS             66380..67600
                     /locus_tag="IWO45_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0331755.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            67590..69176
                     /locus_tag="IWO45_000247"
     CDS             67590..69176
                     /locus_tag="IWO45_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0331756.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            69215..71353
                     /locus_tag="IWO45_000248"
     CDS             69215..71353
                     /locus_tag="IWO45_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0331757.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(71721..72704)
                     /locus_tag="IWO45_000249"
     CDS             complement(71721..72704)
                     /locus_tag="IWO45_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0331758.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            73082..74473
                     /locus_tag="IWO45_000250"
     CDS             73082..74473
                     /locus_tag="IWO45_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0331759.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            74489..75529
                     /locus_tag="IWO45_000251"
     CDS             74489..75529
                     /locus_tag="IWO45_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0331760.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            75548..76633
                     /locus_tag="IWO45_000252"
     CDS             75548..76633
                     /locus_tag="IWO45_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0331761.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            76666..77628
                     /locus_tag="IWO45_000253"
     CDS             76666..77628
                     /locus_tag="IWO45_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0331762.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            77621..79048
                     /locus_tag="IWO45_000254"
     CDS             77621..79048
                     /locus_tag="IWO45_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0331763.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            79050..80120
                     /locus_tag="IWO45_000255"
     CDS             79050..80120
                     /locus_tag="IWO45_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331764.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            80107..81528
                     /locus_tag="IWO45_000256"
     CDS             80107..81528
                     /locus_tag="IWO45_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0331765.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            81541..82548
                     /locus_tag="IWO45_000257"
     CDS             81541..82548
                     /locus_tag="IWO45_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0331766.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            82560..83564
                     /gene="neuB"
                     /locus_tag="IWO45_000258"
     CDS             82560..83564
                     /gene="neuB"
                     /locus_tag="IWO45_000258"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0331767.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            83561..84709
                     /gene="neuC"
                     /locus_tag="IWO45_000259"
     CDS             83561..84709
                     /gene="neuC"
                     /locus_tag="IWO45_000259"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0331768.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            84682..85359
                     /locus_tag="IWO45_000260"
     CDS             84682..85359
                     /locus_tag="IWO45_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0331769.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            85349..86491
                     /locus_tag="IWO45_000261"
     CDS             85349..86491
                     /locus_tag="IWO45_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0331770.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            86504..87481
                     /locus_tag="IWO45_000262"
     CDS             86504..87481
                     /locus_tag="IWO45_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0331771.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            87481..88389
                     /locus_tag="IWO45_000263"
     CDS             87481..88389
                     /locus_tag="IWO45_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0331772.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            88390..89142
                     /locus_tag="IWO45_000264"
     CDS             88390..89142
                     /locus_tag="IWO45_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0331773.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            89147..90094
                     /locus_tag="IWO45_000265"
     CDS             89147..90094
                     /locus_tag="IWO45_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0331774.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        30048 a        14583 c        18621 g        26929 t
ORIGIN      
        1 tactttgtct ttttacactt aatatttttt tgccaatttg gaatcaggat gttttgataa
       61 ctatcgtttt caattgatgg aagctatatt gtttgctatc agtgaaataa ttatatggta
      121 aacagtgttc tttttcttgt ttccattata aaaatcgtat cgggattttt cgttctttta
      181 aattgaacat ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt aaatagttga
      241 cgaaacagaa ataaatacat agtattatgt tttgaagcct aagtacaata gtctatagaa
      301 aaggagcgat accattggca aaacgcattg accgaattta tatgtatgtc aaagaaaaaa
      361 ccgctcattt aacaaaggca gaatatgatc aaggagtaac aacacaggaa attgctgaag
      421 tgttagggat tcagcgaaca aatagcagta aagatctgaa tcagctagtg cgcgaaggaa
      481 aactgataaa gacagacgga cgaccagtac gttacatata tcaaacactc gttacgcacg
      541 taaagccagt tacaaaacat gtagtcagtt acaaagaaac gccggtctct gaagaaaaaa
      601 atacactcca aatagatact aaggatatct ttgcaaaaat cattggagct aacggcagca
      661 tgaaaaattc agtggaacag gcaaaagctg cgattttgta tccgccaaga ggattaaatt
      721 gtttgattac aggacctacc ggatcaggaa agacttattt tgcacacgcg atgtatcatt
      781 ttgcaaaagc gcatcatgta atagatgagg aaacggaact gattgttttc aattgtgctg
      841 attatgccaa taaccctgag cttttgatga gtcatttatt tgggtacgca aaaggtgctt
      901 ttacaggcgc agatgaagaa aaaatgggga ttatcgatca agcggatggc ggtatgctgt
      961 ttttggatga gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt tactttatgg
     1021 atcatggaac ttacagtcgt cttggggaaa caacgaaatc ccatgaagca aatgtacgga
     1081 tcgtaggtgc gaccacagaa gacccaggtt cttctctatt agaaactttt gttcggcgta
     1141 tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag aaagtcgatt
     1201 tagtaaaaat catgattgcc cacgaagcaa accgcacgca gcgaaagatt tctttgacag
     1261 aagatgtagc aaaagcattg atcggcagtg tgacctatgg aaatatagga caattgaagt
     1321 caaatatcca gttagtatgt gcacgaggat ttctaaatca tatgcaatca cccgaaatct
     1381 cgatcacaat cgatgatttg acagaaggga ttcgtagcgg attgattcaa ttagcaagca
     1441 accgcagtgc aatggcagag ctttctaaaa tattggagcc aaagataact gtagcaccaa
     1501 atgatacgat catgaaaatc caatcggact cctatgagtt accttataat ctatatgata
     1561 tcatcggaga taaagcagct ttactgaagt cggatggttt ggatcaagaa gcaatcaatc
     1621 atttcatttc tacagatatc aacatccatc tgaagtcttt ctataaagac catggttttt
     1681 cttttaatgc agacagcaaa ttagcagaat ttgtagatcc gaaaatcata gaagtgacca
     1741 atcagattta tgtaatggtc aaaagatctt taccatatga gtttcagcaa aactttattt
     1801 atgctatgag cttgcatatc agttcttttt tgaaaaaaat caacttagga gaagaacggc
     1861 atacgaatga taacatccgt gagatggctt ttgattttcc agatgaatat gctgttgcga
     1921 aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga gattccagaa agtgaagact
     1981 attacttgac cgttctactc gtctcgttac gggcaaatca agcaagcggc agaatcggtg
     2041 tagtcgttgc agctcacgga aacagcacag caagcagtat ggtccaagtt gtccaacagt
     2101 tgttagatgc agagaacgta cgagcagtag acatgccttt ggatatggat ccgaaaacag
     2161 cattggaccg aattgaacgc aatgtacaag aagtagatga aggaagcggg gtcatactct
     2221 tagtagatat ggggtccctt gcgtcgttta attcgcaaat tcaaagagac actggaattc
     2281 cagtacgtac tgttgatatg gtaacaactt ctctagtttt ggaaactgta cgaaaggtct
     2341 cagtattagg gacagatcta gatatgttgc atgaatcatt gaaaaatttt cgaggttacg
     2401 cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc tattcataag aaaaaagctg
     2461 ttttggctat ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa ttgatcgaac
     2521 gagcggtaaa caagcgtcaa gaaacagaat tagaagttct agctttgtcg attgtggaag
     2581 tgaaagaaga actgccagtt atccaaaaag aatatcagat catagctact actgggatta
     2641 ctgatttgaa tatccaagcc ccatttattc ctttagaacg ttttattgat caaaatatcg
     2701 aagtcatttt ggatcagttg ctgatggaat cagaattgga ggaaactgaa ttcatctccc
     2761 tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat ctctgataac ttcatcttta
     2821 ttaatggtag taagttaatc gatccaatgt ggcaattttc tacacaaatc tctcagacga
     2881 cgggaatcgg cgatgaagaa tatggattca aaatcaacct agtgatgcac acagcaggaa
     2941 tgatcgaacg tattattcgg aatgaaccat tgactgtgga agaaaacgaa ttgacaaata
     3001 caacgaatga tcctctttat agccaattgg cagcctcagt tgttctgttg gaagatcaga
     3061 taaaagtaaa agtgccgata gaagaaatgt attatctgct acgtctagtc cataatcagt
     3121 tagataaaaa agaatacact gtgccatgaa tacacaaaaa cgatacactt ttatagtgta
     3181 tcgtttttgt gtattggtta agaatccttt aaattcaatt agtgtatcgt aaaaaaagtt
     3241 ggcacgcttt ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata
     3301 ggggtttctg tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg
     3361 tatagatgat cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt
     3421 tgaacgtatc attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact
     3481 aaaagaagct gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca
     3541 aatctatcat agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca
     3601 agatgttgaa gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat
     3661 gagttttact gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat
     3721 tgattccttt cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc
     3781 tgacaggaaa atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg
     3841 gttgttggaa aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa
     3901 tttgttacaa caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca
     3961 tggacaattt gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa
     4021 cgtcaaagcc gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga
     4081 agaagcggtt gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg
     4141 cggaacaccg ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat
     4201 cgtcagcggt ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga
     4261 atctgctcat gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa
     4321 accggaagaa ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa
     4381 aggagcactt ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg
     4441 tgttgactca cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc
     4501 aaaccgtatt atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat
     4561 cgaacaagct gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga
     4621 agtagcaaaa gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga
     4681 agacgtattg acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat
     4741 ggcgcattcg gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt
     4801 tgaagcgttt gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa
     4861 tgattcacgc gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc
     4921 gtaataaata atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta
     4981 gtcgtattag ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat
     5041 caaccactag tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc
     5101 gtacttggcg gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta
     5161 gcaccagatg ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa
     5221 ggtgttaaag gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg
     5281 ttcttaacaa tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct
     5341 gctgaagaag gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa
     5401 ggggttcgta tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa
     5461 tcattccttg aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg
     5521 gtcgtagctg taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca
     5581 ttcttcgtta ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt
     5641 gctttaggtg ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca
     5701 aataatggag gcggaagcaa ctctggtgac cctttaggcg acattttgaa tgactattaa
     5761 tcttgaagga ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt
     5821 tagctgttgc gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa
     5881 acggtggctg ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag
     5941 aacgttctgc agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt
     6001 ctccaatttt aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg
     6061 atgatgtagc gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg
     6121 acccagtgtt ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta
     6181 tgggaggaaa tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg
     6241 cattcatgtg gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc
     6301 tttctggcgg attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat
     6361 tagctgccct tgtacaaaga tgggtatcaa tcaaattctt accagttgta tcaactgtaa
     6421 aattagataa aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca
     6481 agagtgcatt tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat
     6541 tacaagacaa cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat
     6601 gtatgtggtt gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag
     6661 tcggtgttgt tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt
     6721 gtcccgagct ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg
     6781 gttcaatcaa tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg
     6841 acagattacg ggcaaatcat gataggtgat aatggttttg agttttacca tgcgcgagat
     6901 agacgcaaat atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg
     6961 ctaaagggaa aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca
     7021 ttttcttcca aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat
     7081 agaatggtac aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt
     7141 aaacgcaagt aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt
     7201 gcagaagaaa atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta
     7261 aaattgatta gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga
     7321 gccgtgatcg gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta
     7381 acgaatatca ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg
     7441 ggaccaaaag ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta
     7501 gcagttgtct catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa
     7561 aggagttgca gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt
     7621 tttccggaaa tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa
     7681 gcgtgcaaag aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa
     7741 atgcaagaag aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta
     7801 acgaacttta caggtacgac aggattagct gttattactt tggaaaaagc attttttatt
     7861 actgattttc gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa
     7921 aatgtcggac ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca
     7981 ttagcttttg aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc
     8041 gatgcggaat tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa
     8101 gaagaaatcg ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc
     8161 ctgaaaatga tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat
     8221 atgcgttctc ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt
     8281 tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca
     8341 ttggactttg gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt
     8401 ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca
     8461 gttattgatg ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat
     8521 tatatttcaa aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt
     8581 ttagagatcc acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga
     8641 aatgtgatca ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag
     8701 gatgacttgt tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg
     8761 atcatcttat agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt
     8821 gttatagaaa atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga
     8881 taataaagtt gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt
     8941 cttaccacgt gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca
     9001 aagggaggaa taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt
     9061 taggagagat cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta
     9121 aagtagaagg cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag
     9181 gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag
     9241 atctttactg ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg
     9301 aacgggtcag ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc
     9361 atgtagtagc tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag
     9421 acgaagagga agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg
     9481 caggctttat ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac
     9541 gcaatcgaat tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt
     9601 tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt
     9661 gaaaaacatt taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc
     9721 ttgcgtatcg cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta
     9781 aatgaagcaa tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac
     9841 ggtgttttat ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct agcttttttc
     9901 tatgaatcat cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa
     9961 aaaatatctg ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa
    10021 atgcaggcag agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga
    10081 ttagtcgttt tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa
    10141 cgtgtagcaa aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct
    10201 gaagacgaac tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata
    10261 ttggtccaat taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc
    10321 cctgaaaaag acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa
    10381 ccggacaaga ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc
    10441 gatccggcgg gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg
    10501 gctcagctac tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat
    10561 ctggctgaac tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt
    10621 gtcacaaaag aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat
    10681 gctaacggta agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg
    10741 atcactcctg tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc
    10801 gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc
    10861 actacattaa cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta
    10921 tatttaacag gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc
    10981 ttaaaagatg accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta
    11041 cgattccagc caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa
    11101 gcgggaggtt cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta
    11161 tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct
    11221 aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg
    11281 gttatccgcg atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg
    11341 ttcccaacag ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga
    11401 gtagaagaga tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag
    11461 gatttatggc cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg
    11521 gtgatttcat ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt
    11581 cgggctgcca ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg
    11641 atgcgcattc aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc
    11701 aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg
    11761 tatgaaggtc agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag
    11821 caaatttatc atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg
    11881 gctccgcttt atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta
    11941 gaagagaaaa tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa
    12001 tctcttgatt tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta
    12061 aatgatcaag tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat
    12121 tatcaagatg gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat
    12181 ggaaaatcca acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga
    12241 acaaggagac gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag
    12301 caaacattgc caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga
    12361 atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact
    12421 tttcagctat tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata
    12481 caacggatag aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa
    12541 tacgtccctt attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg
    12601 tctatcaagt agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt
    12661 tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg
    12721 gcgaagcatt agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca
    12781 gtatggcaca cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg
    12841 cgggatcaca aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc
    12901 tttctttaga agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg
    12961 ccttgctggc tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac
    13021 gattagccga acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg
    13081 gtacaacgaa aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt
    13141 acccgcgttt attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg
    13201 ccaacaagat catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc
    13261 gccagatgat caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt
    13321 agatgtatta gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt
    13381 gatggctggt ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat
    13441 tccagtgagt agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg
    13501 attaaaatta gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt
    13561 agatattggt gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat
    13621 gagttatgct ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag
    13681 agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg
    13741 aattccagaa gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc
    13801 attacatgct attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga
    13861 agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa
    13921 agttttagaa gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag
    13981 ttattctccg atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt
    14041 acccgaaagc ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca
    14101 cgaacagttt gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt
    14161 cttctttatt ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg
    14221 aagacgtaat gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag
    14281 atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc
    14341 aagctacgat ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg
    14401 gcggctaccg ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa
    14461 aactattaaa agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa
    14521 cattgccggg aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat
    14581 tattttcgat tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg
    14641 cagttttatt gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct
    14701 acaagaaatc agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga
    14761 aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt
    14821 aggtctgtta gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg
    14881 tgtgcttgaa gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga
    14941 attagggatc gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg
    15001 aaaaaatgtt tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg
    15061 cagttattta gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc
    15121 acatcttgct ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta
    15181 tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa
    15241 tgaaaaatcc tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc
    15301 tgctgatttg caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa
    15361 ttatcaaaaa attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca
    15421 aaacagtgta gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga
    15481 tgtggagtat gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc
    15541 aatcggcgat atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga
    15601 agttacgcaa cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga
    15661 gtcaatattg gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga
    15721 aggtcaattg gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca
    15781 agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct
    15841 gcatgaattg aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga
    15901 agaaaataga caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa
    15961 tccaggagag ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat
    16021 gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga
    16081 tgaagtggat acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca
    16141 aatatcgaaa tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc
    16201 cgactatcaa tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc
    16261 agagttgaaa acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat
    16321 tacaaaactc acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc
    16381 cacctcgcag tggttcaggt ccttttcagc agctgatagc tatattttat ttttacatga
    16441 cactcattac agcagtcaat acaacagatc caatcgttaa aatcagcatt gtccaaatga
    16501 caaattttgt tactttagca aaagtacttg tttgtttttt ttcacgcatt gttttcaacc
    16561 tcgtcattaa ttagttgatt caagtgtaca atcttttttc catttccaca agagggcttt
    16621 tttataaaaa cttccctttt tttaaataat aggtagttac gatacaaata aataatgtta
    16681 atagaactag ccatataaag gcatcatccc tttcagcaat aggtaactta atgttcattc
    16741 catagatact gccgacgatt gtcggtatag ttaaaataat cgttaaagaa gttaaaattt
    16801 tcataatatt attcagatta ttcgatacga ttgctgaaaa agtctgattc atttgttcta
    16861 ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc aatacatcac
    16921 gtaaatgatt tttcaaagca tgattttctt ggaaaagttt attttgcgct agttctttca
    16981 atgcatctaa attagcagaa gtcgcttctc tgaaataaac gaggcttttt tggatatcca
    17041 tgatttgata aagttgtttg ttttcagtgg atacttgaag tttggcttcc aaagtatcgc
    17101 attgagattg aagcgtagct aaataactgt tgtaagagag tgtgacctgc caaatgaatt
    17161 gaaagaagat attgagatta ggcgaaatat cactttttgg aaaggtctgg ctaaagactt
    17221 tctttaaaaa cagtggttca tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa
    17281 taatagagag cgggtatgta tccacttgaa aataaccgct aggacttgtt ttagcaaaag
    17341 gatattgtaa caataataac gctggctttg tcaactttga ttgattgaat tcttctgtac
    17401 gggaattttc tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt tcaaattgat
    17461 gtgtcagctg atgaatctct tcttgattag gacgttctac acaataccag atcgtttggt
    17521 cagtttcctt tccggtatta cgcagtttct cattatctaa tgtaaaatat ttgatcatga
    17581 ttttcctcct gttgcctgtt tcttttatta taacggatag ggaattggaa tacatggaat
    17641 gtgtttgtaa gatatagaat ataactgcat aaatttaaat aaaatgaaac agttgtgtaa
    17701 agtatataca gtatacttga agcaacgata aaaaaggagt ttacgttcat gggcacattc
    17761 acaccactta tcttgttttg gtatatttac ccggtaatcg ttttgtttgg ttgccaattt
    17821 cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa aagcccctga tttggcgatt
    17881 ccttttttgt ggggcggtat gcatgcattg tctagaaata cagggacaat ttctatctta
    17941 ccgtttttct ttatttctgt tttgctaatg ggaatttgta ttgctgtttt ccaagcatat
    18001 tattatgaag aaattattta tccgcgttat ttcaagatga cttggcgttt ggtttttctg
    18061 ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca tactttctta cctatgaaaa
    18121 aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa
    18181 tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg
    18241 attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga
    18301 attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga
    18361 acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat
    18421 gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc
    18481 acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg
    18541 acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt
    18601 catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc
    18661 agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga
    18721 ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa
    18781 acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt
    18841 gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt
    18901 gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa
    18961 ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat
    19021 gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa
    19081 gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca
    19141 gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt
    19201 ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta
    19261 cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc
    19321 ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg
    19381 attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatccggtta
    19441 ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc
    19501 aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata
    19561 cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag
    19621 gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata
    19681 agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga
    19741 tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga
    19801 aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat
    19861 tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg
    19921 cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa
    19981 aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat
    20041 taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa
    20101 gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat
    20161 gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt
    20221 atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc
    20281 tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat
    20341 ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat
    20401 actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga
    20461 taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat
    20521 tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa
    20581 tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg
    20641 tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt
    20701 tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga
    20761 gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa
    20821 ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga
    20881 tatctacacg actttagaca gcagactaca aagttattta gaaacactga tggatcaagt
    20941 aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga
    21001 aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg
    21061 aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat
    21121 gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt
    21181 ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa
    21241 aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact
    21301 ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag
    21361 tactcattcc ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga
    21421 ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc
    21481 tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt
    21541 ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc
    21601 aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta
    21661 cggtagtgct tatggtgttg tacctggata caatgtatcg gcaaaaaccg gaacagccca
    21721 aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt attcgattgt
    21781 ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga aacaccctaa
    21841 gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac gggcaatgga
    21901 ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag tatccgtagc
    21961 cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa gtggactgca
    22021 acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca acggtgatca
    22081 gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga tgcctgacgt
    22141 aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta ttgaagttag
    22201 ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa aaatcactga
    22261 agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa aagagcatgg
    22321 aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt gccgcaatgc
    22381 cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt gaggagggac
    22441 caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg tttctgatcg
    22501 gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact cctactcttt
    22561 tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat gattttataa
    22621 aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt ggacagattc
    22681 tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt gtattgaatt
    22741 tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc ttctggcttg
    22801 ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt gctggcttag
    22861 gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag tatgatgtat
    22921 taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac aacagaaaac
    22981 ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt ttagcggcaa
    23041 tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc tatgttatgg
    23101 aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa cgaattttta
    23161 aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg aagattgata
    23221 cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc gtttggtagg
    23281 agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct tgtcttagga
    23341 ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc acttgtcacc
    23401 gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct ttctttaggg
    23461 ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt ttcattgatg
    23521 gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca agaaatgggt
    23581 attccggtaa ttacggaagt tgagctagct tacgaagtat cggaatgccc aattattggg
    23641 attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact actgaatgca
    23701 ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc aagtggtgta
    23761 gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt ccaactcatg
    23821 ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga agcacatatt
    23881 gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca aaaaaacatg
    23941 acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga attagcgcaa
    24001 agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga cggtgtgtat
    24061 gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc tgaacttggc
    24121 gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc aaaattgtat
    24181 ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt accacatcgt
    24241 acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa agcaaccaat
    24301 attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt gttattggct
    24361 ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa aggaatcaaa
    24421 gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa aaaagcaggc
    24481 atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc attagaaaac
    24541 agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga tcaataccca
    24601 aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa atagaaaaag
    24661 tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag ggggacatat
    24721 ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg ctgaattcat
    24781 gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag gaatgccttt
    24841 ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca tcaagacggt
    24901 ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt ttcaaccaga
    24961 tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg cagccaaaat
    25021 gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca ataaattttt
    25081 aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct tttttcctga
    25141 aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata ctaaaaaatc
    25201 agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga tttttggcgg
    25261 tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg aatatattcc
    25321 ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga tcctaaataa
    25381 aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata tggcagaggt
    25441 catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg cagaattcac
    25501 agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg atcatcagac
    25561 taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag ataatgaact
    25621 aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag aattgcaaaa
    25681 gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac gtctttatga
    25741 cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa gaaaaaagaa
    25801 ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg gcaaaaggct
    25861 aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa agaagacata
    25921 acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga ggaacagcaa
    25981 gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca aaagaaaaac
    26041 ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag tcaacggcac
    26101 aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc gctgattttt
    26161 ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc aggcaataag
    26221 acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga aaatgtttgg
    26281 ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca gccaagaatc
    26341 gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt aacagaatat
    26401 aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga aaatggaaca
    26461 gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga agagttcaaa
    26521 gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc agaattacaa
    26581 aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct gatccaattg
    26641 aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa ccaaatgaaa
    26701 tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat ggaagttggt
    26761 atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca atcggaagaa
    26821 acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga ggcagaaacg
    26881 caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc aaatgattca
    26941 agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt aaatgaaaat
    27001 tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat ttatggtaga
    27061 attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc tagttgttcg
    27121 cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca ggaatgtatg
    27181 taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat atcgacagcc
    27241 aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga ggcattatcg
    27301 ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca gaagaaaaag
    27361 caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt gaagtagaaa
    27421 attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat gaagatgtca
    27481 gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa atcgtgtcaa
    27541 tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct cgtggaatga
    27601 tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg atcatccata
    27661 acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg attactcctt
    27721 tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg atcgtcattg
    27781 atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg aagtttacca
    27841 atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta ttaaatacgt
    27901 catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca gaacggacat
    27961 caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg aaagtcgatg
    28021 aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac aaagcaaaag
    28081 atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg acaggcggag
    28141 ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta aatgtaaaat
    28201 tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta ataagtatcg
    28261 tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct gttacgggtg
    28321 aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca tatgagagct
    28381 atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc ggagaaaatg
    28441 taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc ctgggaggaa
    28501 taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat caaagtcatc
    28561 ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa cgttaaaggt
    28621 gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa agctgaaaca
    28681 gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca gccagaagtt
    28741 ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga cggtgcagat
    28801 atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc accaatcgtt
    28861 gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg tccattcact
    28921 tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt gaaagaaaat
    28981 gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga taaaaagaca
    29041 ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt acaaggtatc
    29101 tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt gaaaacagtg
    29161 atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga agatcgcgta
    29221 gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat tgatggagct
    29281 gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga agcacaagat
    29341 gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct aggtacatct
    29401 atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg tatcgatgaa
    29461 tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca atcacctgct
    29521 caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga agaaaacagt
    29581 tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga tgattccaat
    29641 tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac aaaatctcat
    29701 ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag gagaggaaga
    29761 gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt cgtgtgctct
    29821 tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg ttgaatccgc
    29881 tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc gagtagataa
    29941 attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc atttaattgg
    30001 taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact ttcatgcgtt
    30061 ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat tatcctgttt
    30121 tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag atgagctttt
    30181 accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt tgatgactat
    30241 ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc tgaaagaatt
    30301 gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat taagtatggg
    30361 gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc gtgtaggaac
    30421 agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac agaggagcaa
    30481 ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat tatcctgaaa
    30541 caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa tcaactcatt
    30601 ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt cctgtaacgc
    30661 atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa tcacctacaa
    30721 aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa gcaaatcttg
    30781 ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg attgctaaac
    30841 gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa aaacaagcga
    30901 gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat atcaaacgtg
    30961 ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt tcaaccgctc
    31021 aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga tttatactat
    31081 tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta tcttgatggt
    31141 atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac gattattagc
    31201 taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga ttggaccctt
    31261 ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag gtttagcaca
    31321 gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa atctttattc
    31381 tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact ttcgggcaga
    31441 tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag gacaatatgc
    31501 accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa cactgattcg
    31561 acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag aaagaaaaag
    31621 atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa cagcattgta
    31681 tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc ttggaacttt
    31741 agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg gtgatcgctg
    31801 gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa caaagattgg
    31861 aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc cgaaagatag
    31921 ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga tctctgagat
    31981 ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag taaaagtgaa
    32041 ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta tttcagtcag
    32101 aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag aaaagtacag
    32161 ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat ggctttaact
    32221 ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa tcaagacgaa
    32281 gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca aagaaatcga
    32341 gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa tgaattaaaa
    32401 gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt aaaaacaagt
    32461 gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga tgagttagtt
    32521 gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa agcaagaaaa
    32581 atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga agatcttaag
    32641 aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca attagaacaa
    32701 gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt gactgacagc
    32761 catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga tactgatgta
    32821 aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca ggctattgat
    32881 atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact ttgttattct
    32941 taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa gatcctctac
    33001 ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt tattggtcac
    33061 agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca tgttcgtccc
    33121 ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta tggttgaatg
    33181 aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg taatttgcca
    33241 aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca aaaaagacaa
    33301 gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata tgccaatggt
    33361 aatatccatt taggacattc attgaataaa atcagtaaag acattatcat tcgttcaaaa
    33421 tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg attgccgatt
    33481 gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc agaatacttg
    33541 gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga tttcaaacgc
    33601 ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc ttatgaagct
    33661 gcacaagtac gcgtatttgg taaaatggct gaaaaaagct atatttacaa aggattgaaa
    33721 ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat tgaatataaa
    33781 gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa agatattctt
    33841 gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc agcaaaccaa
    33901 ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg ccgtaagttt
    33961 gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga aaatgtaaca
    34021 gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca tccattctat
    34081 gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg tactggatta
    34141 gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa atataaacta
    34201 tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc tggatttgaa
    34261 ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga aaaaggagct
    34321 ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac gaaaaagcct
    34381 gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg tcagaatatt
    34441 ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg tttgtataat
    34501 atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg agtaccactt
    34561 ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat cgaacatgtg
    34621 gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc gaaagaactt
    34681 cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa agaaaaagat
    34741 atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg tcagcgacca
    34801 gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg tggctggttc
    34861 aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa atcagttatt
    34921 tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt aggaaacatc
    34981 attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt atgggtatct
    35041 agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca agtggcagaa
    35101 gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga agatttcgat
    35161 cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata tatgaccgtt
    35221 cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata taacttcatg
    35281 catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt ctacctggat
    35341 tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg ttgcatgcaa
    35401 actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat cattccgcat
    35461 acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca gttaagcgag
    35521 tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg gactgctttc
    35581 atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag caaattgatt
    35641 ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg tgaaatgtta
    35701 aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta ctttgaagtt
    35761 atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt tgctattaca
    35821 gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa agatgtagga
    35881 gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga agacttctat
    35941 cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg tgtgacatgt
    36001 tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac gataccagtt
    36061 atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag ctgctttagg
    36121 tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc aagcttttcg
    36181 aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga agttcgttcc
    36241 atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat tttcaaccat
    36301 ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt gccctacttc
    36361 tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg ttagaacatt
    36421 aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata cattaacagg
    36481 aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt tgcctgtacg
    36541 tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc ctgcgacgaa
    36601 tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct tgaattgtac
    36661 accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca cttcttcttc
    36721 gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt ctttttcgga
    36781 aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat gattttgcac
    36841 catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta caccgaggtt
    36901 ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag cctcaaaaat
    36961 attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta aataatgatc
    37021 aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca attcaaaagc
    37081 agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc cattttttgt
    37141 cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag gtgccatagc
    37201 caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt cagacaattc
    37261 ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt aaaaatggct
    37321 tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt ctttgacaac
    37381 ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag caaaatgctc
    37441 gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac gctgggctag
    37501 gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca tgtggataca
    37561 ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac ggggattttc
    37621 tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac gatctcatat
    37681 tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt ttcatcaagc
    37741 actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg tcggcgttct
    37801 tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg ttcaggatga
    37861 ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc caatacttca
    37921 gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa aacttcccat
    37981 aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt caattgtaca
    38041 ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat catttcactg
    38101 acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac tttcgttgta
    38161 tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt catattcgat
    38221 catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg ttaattgact
    38281 atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa acgaaagatg
    38341 atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg agggatctat
    38401 ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt ttgttcggga
    38461 taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata ttcatctatt
    38521 tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc agatgtaatc
    38581 tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata ctttgagagt
    38641 ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga gagaacggta
    38701 atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct ttatgagaaa
    38761 ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa agctaaaatt
    38821 tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat aggatacata
    38881 gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt tttttattga
    38941 ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa ttattgcgca
    39001 tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag tggctattgt
    39061 atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa aaaagaaggg
    39121 tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg gtattttaga
    39181 cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc gtgaagtaac
    39241 ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa aagcatttgt
    39301 tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg atgcgcccac
    39361 actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg gttatgccgc
    39421 ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc atgaagaaat
    39481 gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc aaatcctcgc
    39541 ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc catttgatgt
    39601 agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga tcaacttagg
    39661 tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa gcacaaaaaa
    39721 atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat ttggttctca
    39781 ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata ttactcatat
    39841 ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta agcaagcaat
    39901 tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa aagaaatgga
    39961 tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag caagaaatat
    40021 ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg agataatcgg
    40081 tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag atggagaaga
    40141 accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag gtgtttacct
    40201 tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt tcccaagaaa
    40261 gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga attttgatgt
    40321 atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt attttgactc
    40381 aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc atgatcgcgc
    40441 gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg cagatgaagc
    40501 agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat tgttgtatga
    40561 agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa gaaaaaatgg
    40621 cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt tgtacaagta
    40681 tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc ttctcttatt
    40741 caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt ttctcctaca
    40801 ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga accaggtgaa
    40861 gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt agcagatgtt
    40921 gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg cggagccaac
    40981 gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg tacaccagga
    41041 agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa aacaatggta
    41101 tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga tcaaattctt
    41161 catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga tcgttccctt
    41221 gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac agcagaggac
    41281 gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa gaaagatgac
    41341 tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt taatcaactg
    41401 actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt aggaagtctg
    41461 gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa aaagaaaatg
    41521 gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac aggtcttcct
    41581 tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg ttcgggaaga
    41641 actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa taatctaaga
    41701 gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt tttatacgga
    41761 ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt aaataaaaag
    41821 tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga aaaaataaga
    41881 aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg caaataagcg
    41941 cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg aggggttatg
    42001 tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa ggattgacag
    42061 caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga tatttaagtg
    42121 aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt tactctttgc
    42181 caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa gtgacttttg
    42241 aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca aaagctgcga
    42301 ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt acagggaagt
    42361 cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt tctgcagatg
    42421 cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg ctctttggac
    42481 acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt cccggtctaa
    42541 tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta ccgccggaag
    42601 ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt ggagaaagtg
    42661 cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct tcagaaagtt
    42721 tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat cttgcttcac
    42781 gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa gcgaatcgtt
    42841 tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt tatgatgcgg
    42901 aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa tcttttttac
    42961 attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt tcccttcaag
    43021 tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga ttcatggaca
    43081 gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg tcacaagatc
    43141 cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct ctttccgaaa
    43201 ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat gcttatttcc
    43261 agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg ccagatgaaa
    43321 tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac cgtatttata
    43381 atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat cgtgtgagag
    43441 aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt aagagggaat
    43501 tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt gagattcctt
    43561 ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa agtgagcctg
    43621 ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact gcttcaagca
    43681 tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt aatatgccac
    43741 tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa aactatcaag
    43801 agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac accttcagtg
    43861 atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca agtaccatga
    43921 ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat atttatcaaa
    43981 atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa aagtctgaaa
    44041 ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga gtagctgcca
    44101 aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg atccagatgc
    44161 agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa gaatatgaga
    44221 tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt ttttcagcct
    44281 atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat gatgagatcc
    44341 cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt tctattgatg
    44401 aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg tcgattatcg
    44461 tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta gacggattga
    44521 tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa atttatcgtt
    44581 tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat caggtaaaaa
    44641 ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa ataaaaaaat
    44701 gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat aaaaaagagc
    44761 taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc ttgcgttata
    44821 taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat catgttagtt
    44881 tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc agcagaagaa
    44941 aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa taatttagaa
    45001 tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa agctcaattt
    45061 gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca agactatggc
    45121 atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata aatcaagtat
    45181 gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc atggggttaa
    45241 tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct gctaaaatcg
    45301 gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt atacaagcgc
    45361 atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg caagtgacat
    45421 tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact gatttagcaa
    45481 aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt cttttttgaa
    45541 cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa ctctctttat
    45601 ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga tggagaaata
    45661 catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt tgcgtgatgc
    45721 ttttatcggc acactgcccg caacaatggc gggatcagtc gctgtaatga tcaatgcaat
    45781 tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag cagcagacaa
    45841 aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct ggaacggtac
    45901 actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg cccgagctta
    45961 tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc aaggtatttc
    46021 ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg tcgcagatgc
    46081 aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag ttggattatg
    46141 gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta tgatcatggg
    46201 agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga tcaaaatgcc
    46261 tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag cgacgatcgc
    46321 tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg ggcaactgat
    46381 cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc aagggttggg
    46441 agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta ttcatggacc
    46501 aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa tcaatattga
    46561 tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag atgcaatcga
    46621 agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat ggtttggcgg
    46681 atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac gtgcagatta
    46741 tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg aaccagtgat
    46801 gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga ttgctccagt
    46861 agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc cggtatcgca
    46921 gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag gagcggattg
    46981 gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt gggcaccatt
    47041 tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag atgaacgtga
    47101 ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga actgaaatga
    47161 ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa ggatacaatg
    47221 aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc tttgaaacaa
    47281 gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa gcacagcctt
    47341 ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa aaagatagga
    47401 tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg atcgtgttgt
    47461 atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca ggtctttttc
    47521 taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa gcaaaggaga
    47581 aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt tttacgataa
    47641 agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag taactagaaa
    47701 acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg ctcgcataaa
    47761 aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa cgaagaatga
    47821 attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg ttcattacac
    47881 agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt taggaacaat
    47941 cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg aagcaacgta
    48001 ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc tagaaggaat
    48061 ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt aggaaggggc
    48121 agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga aatgtttgat
    48181 ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg ttttcgtaca
    48241 cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct aacaagtcaa
    48301 gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat ggccggcatt
    48361 ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca gatcatggaa
    48421 aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc gccattctcc
    48481 tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag ttatcaacta
    48541 aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg tatggatttt
    48601 gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa ccaacgagct
    48661 ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg caattaccag
    48721 tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta tcagcttgca
    48781 acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt ttgcagcctt
    48841 gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc atgcggctac
    48901 ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat gatcacacct
    48961 tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc atttaaaaag
    49021 agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg gaatgaagga
    49081 atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga acaaacaaaa
    49141 ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg gtatcgtaaa
    49201 gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga agaaacgtta
    49261 agctcaatct tgccacagca acagccaatt cttaatgact atttttgata aaaaacgggt
    49321 gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa ggaaaatgtt
    49381 aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt ttagggtttt
    49441 cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt ttagatgaat
    49501 ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag gacctcccta
    49561 cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa cgttttgcct
    49621 aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa caagctacaa
    49681 ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct cctaagcgat
    49741 aggtcgctgg ttcaattcca gtcaggaacg tacaaataac ccctgtatcc tttgcggtac
    49801 agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt
    49861 cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg tatgtcttag
    49921 tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag aaaaacttac
    49981 taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac gatttcgatt
    50041 ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc ggtataactt
    50101 gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta tttttagtaa
    50161 tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa aaaacggtgg
    50221 ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt ctatgcttta
    50281 tattattttt tgcatgtgtg cgaaagtata ctcaagccca atcgccattt cggaaaatcg
    50341 gtactgtaga gccatcttca cggattccgt cgatatccat cttgtcagat cctaccatga
    50401 aatctacgtg ggtttgactt ctattcaatc cagcttctgc taattcttca tcggacattt
    50461 ctgtaccgcc tttcacactg aaggcataag cagaacctag tgctaagtga ttcgatgcgt
    50521 tttcatcaaa taatgtattg aaaaagatga tgccagattg agaaataggt gaagggtcag
    50581 gtacaagtgc gacttctcct aaacgacgcg caccttcatc tgtatcaagc agttttgcta
    50641 agacatcttc tccttgttcg gcagaaaaat caacaacttt tccatcttta aaagtaaatt
    50701 tcatacccga gatgatggtg ccagcataac tcagtggctt tgtacttgaa atgtaaccat
    50761 ctactcgatg actgtcaggt gctgtgaaca cttcttccgt cggcatattt gccatgaatt
    50821 tttctccacg agcattatag cttccagcgc cttcccaaag atggttttta ggcaatccga
    50881 taatgatatc tgtaccggga gcagtatagt gaagggcaga aaattgttct cgattcaatt
    50941 cttctgcttt ttttgctaat ttttcatcat gcttcttcca tgctaagaca gggtcttctt
    51001 catagacacg agtcgttttg aaaatctgat cccataatgc ctcaacttgt ttttcttctg
    51061 gcagttctgg gaagactttt gctgcccatt gtttaccagc cgcagccacg acagtccaac
    51121 tgactttatt tgcttgagtt gccttgcgta aattcattag tgctttgcca ttcgctgatt
    51181 gataagacgc tactcgatgg ctatctacac cagcaaatgc atcgggatcg gcagaaacaa
    51241 cgctgatcct gcttgctcct ttttcaagcc attcatccgc ttgatcgatt ttagattgtg
    51301 gtacattctc aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg atctggtcgt
    51361 cagtccattg aacgataacc tcagcggctc ctagtttata agcttcttgt gtgatcaatc
    51421 gagcaagggg tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct ttttctgtgg
    51481 ctacgccagt ttctgcaatt aagcgtgcat atttttttag aagttcatta aaatcagata
    51541 acataataaa aattcctcct caccattttt tctggtaact attagcatga taccatttaa
    51601 ttatgattca aacaatgagg ctttaaataa aaaaaggagg ttattttttt aaggttaagt
    51661 ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa aactaatttt
    51721 gcatatcaaa aaaacatgtt acaatgaaat taatcaatgg tggaagcgtt ataaaaaaat
    51781 taaggagatt aaaaaatatg gcaagaaaaa agacgattac tagagaacag attttggcag
    51841 ctgcttatga agtagctgcc accgatggat ttacacgatt tactgcgaga aacatcgcga
    51901 acaaaatgaa ttgttccacg caaccgattt atcttgagtt taaaaatatg gatgacttga
    51961 gacaagctct ttttgagcaa atctatgatc atttacaata tgaagtgttc actgttgagc
    52021 atacagggaa tacgattatc gatcttgctt ttaattatat ccattttgca agaagagaga
    52081 agaagttgta tcgttcactg tacttagaag aatatggtgg aggaaaagag atgcagaatt
    52141 tctcttatac ttactttgtg aatacagtga aacgcgatcc agcttatgtg aatctttcag
    52201 aagagcagat cgaatctttg tataatggga catggatcat agctactggg atcgcagctt
    52261 tgatgtcttc aagcatcatc catccaactg atgaacagat tgaaaagatg atccaagaca
    52321 gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat cgattgaata tactcaaaag
    52381 ttccacaaat cagtaggtaa caagagatgt gtcaaaataa ggaaaagttc gtttttctta
    52441 cagcgtatgc atttttaact cccgaagaac tcttccatga atgcaatgaa taaaagagcc
    52501 atacagattg aataaatctg gatggctctt ttattgcaag tatattaaat ttttgcgact
    52561 agttctttag cgaaagcttc taaattttcg atatcttctt cttcagcatt cagatccact
    52621 ttaacatttt cggctccttt tgatgcgcct gtttttgcaa aagcagcatc gaaatcatcc
    52681 accgatttgc agaattcatc gtaaaaagta tcaccagatc cgcagacacc gtaaattttt
    52741 cccaatagat ccaattcttg caagtcttca taaaagtcta cgatctcatc aggcagatcg
    52801 ccatcatcgt atgtataagt agctactacg caaatatctg cctcctcgaa ttcatctgca
    52861 tctacttggg tacattcgtt gatttcgact tcgatatcca gattttcaaa tgcttctgca
    52921 acaatatcgg cgatttcttc agtgtttcct gtcatgctag catatacgat ttttgctaaa
    52981 gccatgatgt gcctcctcgt ttataattat ttaacaatta tgaattatac caaaacatca
    53041 ttgaaaagaa aataaaacct aaagacacct ggggaatgtc atttggctaa atgtatgccg
    53101 ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa atatgattac tttaaaatca
    53161 gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat tagcagatgt acataaaaat
    53221 ctgcgtgatt ttataaaacc aggaatcaca agttgggata ttgaggtatt tgtcagaaac
    53281 tatatcgaga gtcatggcgg aattgctgcg caaattggct ttgaagggta tgaatatgcg
    53341 acttgtatca gtataaatga tgaaatttgt catggattcc ctcgtaaaaa gcctttgaaa
    53401 aacggtgatt tggtaaaagt cgatatgtgt atcgatttga aaggcggcat ttctgattct
    53461 tgttgggcct atgtagttgg agaatctaca cctgagatcg accgattgat ggaagtaacg
    53521 aagaaagctt tgtacatcgg tattgagcaa gcacaagtag ggaatcggat tggtgacatc
    53581 ggtcatgcta tccaaactta tgtagaaggc gaaaatctag cgatcgttcg tgattttatt
    53641 ggtcatggcg ttggtcctac gattcatgaa gaaccagcag ttcctcatta cggagaagct
    53701 ggaaaagggt tgcggttgaa agaaggcatg gttatcacga tcgaacctat ggttaatacc
    53761 ggaacttgga aaatgaaaat ggatcctaac ggctggactg cgtatacaag agacggcgga
    53821 ctttcttgcc aatatgaaca tacattggct attactaaag atggtccaag aattttgaca
    53881 tcacaaggag aagaaggcac ttattgatct atccagtcaa taggtacggg agaggtacat
    53941 gaaagtttgg aacaaaataa aacaaaatga aaatctgatg cgctttattg agacgactca
    54001 aaagcgcatc actgattcag aaatgagtac gacctctgtc gtcgttgctt attatttact
    54061 cttgtcttta tttccattga ttattgcttt tggtaatatt ttaccgtttc tccacatcga
    54121 tcaagagact gtactgacct atttacgaga agtgattcct gagaccgttt accagtttat
    54181 cggtcctgca atcaaagacc ttttgacaca aagttcagga ggcttgttat ccgtttctgc
    54241 attagcggct ttatggtctg caagtcaaag tatcaatgct ttgcaaattg cgatgaataa
    54301 agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc cgtttcttct cactaattgt
    54361 cattgtcttg ttcatgattg ctattagtgg ggtaactatc gttcttgggt tgggacagct
    54421 gattttggat gctattcagc caatcgtaca aatacccgtg aactttatcg atcagttcca
    54481 aactttgaag tggccaatca cattggtagc tttatttgtc atcatgttct tgatttattt
    54541 aattgtccct aatgctcagt tgaagttgaa gacgatcatt ccgggcacga tcttcgctac
    54601 tgtcggctgg atgctgttat cacaagtgtt tagtatctat gctaggtatt ttgcaacgag
    54661 gatcagcggt tatcagatca taggaagttt tattgtactc atgctttggc ttaattttgc
    54721 tgctacgatc atcattttgg gcggaatcat caatgcagtt gttcaagaat acgtgacggg
    54781 tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg ttcaataaat tgaaaactaa
    54841 attttctcat aaaaaaaagt agaaacaaga gttcttattg taaatttata tcagtaattt
    54901 ataataatta tgactcttaa ttaaagtagg tgcccatatg cttgtttctt ttagtatggt
    54961 tataagattt tttctgacaa ttttattatc actgatatta acacctgtat tcaaaatcat
    55021 ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga agaataaata aagttccaat
    55081 gccttcagcg ggcggactgc ctatctttat ttcatttgtt atatcgactt tattcttatt
    55141 ccgtaaaatt atccctcaat cgtatattgt accgatcttg attgcttctt tagtaatcat
    55201 agtcacagga gtgctcgatg ataaatatga gctgacacct aaacaaaaaa gtatagggat
    55261 cttgattgct tctcttatta tttattttgt agcagatata cgtattgatt ccttcacgct
    55321 tccttttatt ggatatatcc aactaggctg gcttagtttt cccgtaacga ttttttggat
    55381 cttcggtatc acgaatgcgg tcaatctgat tgacggtttg gatgggctgg cggcagggat
    55441 ttcattgatc ggtttaatta cgataggaat cattggatat tttttcctcc atgcttctac
    55501 agtgtatatt ccagttgtta ttttctgtct tgtagcaagt attatcggtt tttttcctta
    55561 caatttttat ccggcaaaga tttatttagg cgatacagga gctctattcc tagggtttat
    55621 gatggccgtc ctttcactac aagggctgaa gaacgtgact tttgtttcat cgatttcttt
    55681 gctgatcgtc atgggagtgc cggtaacaga cacgtttttt gctatcatta ggagaaaagc
    55741 aaaccgtgtt tctttttcta cagcagataa aaaacatctt catcaccgac taatcagttt
    55801 aggctttact cataaaggtg ctgtcctgac gatttatgcg atggctttga tgttttcttt
    55861 cacagctatg gtcatgaatt atactggaag aatcggaacg atcatattga ttattgccat
    55921 gttgtttgct gcaattttat tatttgaatt gattggtctg atcaatgaga aacatcagtt
    55981 tattttaaaa acattgcgat ttttagggaa taaagagtac cgtacgcaag taaaacagaa
    56041 atatttgaag aaatggaacc gtcatataga tgaataacaa attaaaccag ccgatcctta
    56101 ttagtattgt gacggcaaac agtgataaaa tttttcggac actagataat tttatttcaa
    56161 ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga caacaattca cttcccgagt
    56221 acaaaaaaag actaacagag tataggaaat atccctacat cgatatctat tttcatgatg
    56281 aaaattccgg atttggttat ggacataatc acaatctgat agctagcgat tctcgctatg
    56341 gcattatttg taacccagat attttagtgg atgaagagac agttacagca ttggtcttgt
    56401 tattgaaaga acatccggaa tgtgcgatgt tagcacctaa gattcttaat gaagatggaa
    56461 caactcagca cctcatccgt gagcagctgg ctgtttttga ttacgttttg cgctttgtgc
    56521 ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc tgtgtttgaa tgccgaggat
    56581 tgcctactga tgctaatagt tatgtacgga tgatctcagg tagttttatg gtagctgata
    56641 ttgataaatt taaagagata aaggggtttg atgagcgttt cttcatgtac tttgaagaca
    56701 acgacttgtg tttgagtttt gaaaaagcag gttacaagtt attgtacaca cccttttatg
    56761 cagttatcca tttttacggg aagggtgcgc atcgaaatgc aaaactgttt cgcgtgtttt
    56821 tgacttctat gatgaaattt ttcaataagt gggggtggcg ttttttctga tgaaaccaga
    56881 aatattagta gtcgttgtgc tttatcagca actattcagt caatctccag cttatggacc
    56941 attaagcaaa gcacttagcg aaaagcaaat tcagcttatt atttacgata atagtcctca
    57001 aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt tatcatcatg atccgcaaaa
    57061 tcctggttta gcggtggctt ataattttgc gttgtctcaa gcagatgaag gaacacgttg
    57121 tctcgtcact ctggatcagg atacgaggct agtcgacgat tattttgaac tattgcgtaa
    57181 agtaaccttt acagatgaat gtgttgctgc agttccgatg attttttctg ggagccgaca
    57241 agtttctccc ctatattcag atcgttatat caatcgtaca gctcaagcag tagaagttga
    57301 aggcccaaca gctgaacgaa tcatggcaat caattcaggt gttgcctggt ctgtcaaatt
    57361 tctaaaaaag attggcgggt ttaatcctgc tttttcttta gattttttgg atcattggct
    57421 gttttggaaa gtggatcagt tggaaaaaac tgtggagatc cttcctgtac gaatggaaca
    57481 tgatctttcg gttttagact atgaaaaagt ttcaagtaat cgataccgtt ctattttaca
    57541 agcagaaaat cgattttatc atgaatatga tcaaaggcat ttatccagtc accgacgaca
    57601 attattgtta agaacagcta aacagttctt gaccgtcaaa aaccggcata tctggcggat
    57661 gactttgcgc tcatatgtta agaattggaa ggtgtgaatt cgtgcgttcg gtttgtatag
    57721 ctacctttaa tggaggcaga tatttaagag agcagttaga tagtatttta tctcagcttt
    57781 cagcagaaga tgaagtagtc atttctgatg atgggtcagt agatgataca tgggatatat
    57841 taactgaata cgcagaaaaa gataagcgaa tccagctttt ttcggggcct cgtcaaggat
    57901 tgatcgctaa ttttggttat gcgatccagc agacgaaagg agatctaatc tttttggcag
    57961 accaagatga tgtttggttg gagacaaaag tgaaaaagat cactgactat tttgagcaga
    58021 atccggatca gctagtagta gtttctgatt taatcattgt ggacagcgag ctgcaagaga
    58081 tccatgattc gtatttttcg tttcgaaatt ccaaagaagg atggcttaat aatgtgataa
    58141 gaagtcacta tattggtgct ggtatggctt tccggtcttc tttaaaaccc gatattttgc
    58201 cgatcccgtc agaagtgccg atgcatgata tgtggattgg tttgctggga ggaaagaaga
    58261 ctgggttcct aagagaaccg ttaactctgt accgaagaca tgatttaaac gcgagtgaga
    58321 ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg tgttcatctg attacggcgt
    58381 tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt taaatcctta atatttccat
    58441 gacaaatata acaattgatg agataataac aaagtgaaat ttgaaatcaa aaaggagagc
    58501 agatatccat gaagggaatc attcttgctg ggggcagcgg aacacgtctg taccctttaa
    58561 caaaggcaac atcgaaacaa ttgatgccga tttatgacaa accaatgatt tattatccaa
    58621 tgtctactct gatgttggcg ggaatcaaag aaattctgat tatttcaacg ccacaagata
    58681 caccacgttt caaggaatta tttggaaacg gaaatgattt aggactgcag atcgaatatg
    58741 cagtacaaga aagtccagat ggattggcac aagcgtttat cattggagaa gaatttattg
    58801 gagatgatag tgtctgcttg gtattagggg acaatattta ctatggtggc ggtctgtcta
    58861 aaatgttgca acgagcagct tcaaaagata caggcgcgac agtatttggc tatcatgtca
    58921 atgatccaga acgctttggt gtagtagaat ttgatgagaa tatgcaagct ttgtcgattg
    58981 aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac tggcctttat ttttatgaca
    59041 atgaggtaat ttctatcgct aaaggtatca aaccttcaga acgtggagaa ctagagatta
    59101 cggatgtcaa taaagcatat ttggataaag gaaaactatc cgtagaactt atgggacgcg
    59161 gatttgcttg gcttgataca ggaacacatg aatcattgct tgaagcatct acctttatcg
    59221 aaacaattga aaaacgtcag aacttaaaag tggcttgttt ggaagaaatt gcttaccgta
    59281 tgggctacat cgataaggaa caattgctgg cccttgcaca gccattgaaa aaaaatcaat
    59341 acggtcaata tttattgaat ttggccgctg aataaggagt ccttacagat gaaagtaaca
    59401 gatacaaaat taacagatgt caaaatcgta gaaatggacg tctttggcga tcatcgcggt
    59461 ttcttcacag aaagttattc aaaagcaaaa tttgcagaac atggattgaa atatgacttt
    59521 gttcaagaca accattcact atcagctgaa gctggtgtcc ttcgggggct tcatttccaa
    59581 aaaggagaag ctgcacagac aaaattgatt cgggtagtca ctggagcagt tttagatgta
    59641 gtagtggata tccgcaaagg tagcccgact tatggtcaat gggacggatt tatcttatct
    59701 gaacataatc atcgacagtt gcttgtacct aaaggatatg cccatggatt cgttactttg
    59761 acaccaaatg tgaacttcct ttataagtgt gataattact ataatgcaga agcagatggg
    59821 ggcatcgcat ttgatgatcc agatttagcg attgattggc caattgatcc atcaagagcg
    59881 attatgtcgg aaaaagatca ccgtcatccg acattaaaag aatttgaagc agaaaatcca
    59941 tttgtttacg gagaaatcta agaattaagg agaaattttc ttatgaaaaa catcattgtt
    60001 accggaggtg ccggattcat cggctctaat ttcgttcatt acgtggtaaa caatcatcca
    60061 gaagtgcatg tcactgtttt ggataaactg acttatgcag gaaacaaaga gaacttggcc
    60121 ggacttccta gcgatcgcgt agaattggtc gtaggagatg ttgcagatgc agaattagtg
    60181 gaccgtctag taaaagaagc ggacgcagtt gttcattacg cggcagaatc ccataacgat
    60241 aattcattaa aagatccttt cccatttgta caaacgaata tcattggtac gtatacactt
    60301 ttagaagctt gccgcaagta tgatgtacgc tatcatcacg tatctactga cgaggtttac
    60361 ggcgatcttc cattacgtga agacctgcca ggacatgggg aaggcgaagg agaaaaattc
    60421 actgcggaaa caccatataa tccttcaagt ccatattctt ctacaaaagc tggttcagat
    60481 ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg caaccatttc aaactgttca
    60541 aacaactatg gtccttatca gcatattgaa aaatttattc ctcgccaagt aacgagtatc
    60601 ttaagcggta ttcgtccaaa attatatggc gaaggaaaga atgttcgtga ctggatccat
    60661 acgaatgatc attcttcagc agtgtggctg attttgacga aaggcagaat tggcgaaacc
    60721 tatctgatcg gtgcagacgg tgaagaagat aataagaccg tgatggaatt gatcttagaa
    60781 atgatgggcc aaccaaaaga cgcatacgac catgtaaacg accgcgcagg gcacgatttg
    60841 cgttatgcaa tcgattcgac aaaattgcga gaagagctag gttggcagcc tgagtttacg
    60901 aacttccgtg atgggttggc tgacacgatc aaatggtacg aagaacatga agactggtgg
    60961 aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa atggacaata atacagagga
    61021 aatttcactg tattaatgga aaaacacatg tgaggcaggc gctcgcctgt ctctttttta
    61081 aaataaaaat caaggagcgg ataaccatat gtttttatta actggaggaa atggacaatt
    61141 aggtacagaa ttgcgccatt tattagatga aaaaaatctg ccatatgttt caacagatgc
    61201 aaaagacttg gatatcacag atgctgagaa aacaatggct tatattacag aattaaaacc
    61261 cgatgttatt ttccattgcg cagcttacac agcagtagat aaagcagaag aagaagcaaa
    61321 agaattagat gagaaaatca atgtcgatgg cacaagaaat gtagcacttg cagcaaaagc
    61381 tgtgaatgca caattcgtct atatcagtac agactatgtt ttcgatggaa aaaacaaaaa
    61441 tgtagaatac gaagtagacg accaaacgaa tccactaaat gaatatggtc gtacgaaatt
    61501 attaggcgaa aaagcagtag aagagatttt agaagactat tatatcatac gaacttcttg
    61561 ggttttcggg atctatggac acaatttcgt cttcacgatg caacgcctag cggaaacccg
    61621 cgacaaactg actgtagtaa atgaccagtt tgggcgacca acttggacac gtactttggc
    61681 agaatttatg gtatatgtca tcgaagaaaa agcaccattt ggtatttatc atttgtcgaa
    61741 cgaaaatagc tgttcttggt atgagttcgc aaaagaaata ttgaaagata cagatgtaga
    61801 agttgcacct gttacgtcag aagaatttcc gcaaaaagca acacgtccgc agtattcagt
    61861 catgagcctg aaaaagacag aagcgttagg ttttgtcata ccaacttggc aagaagcact
    61921 tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa tttccttttt atcaaataga
    61981 atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa aagccaaaag
    62041 cgaatcaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc tatcttattg
    62101 gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca cttggtgttt
    62161 gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt tttggacaga
    62221 atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc tcacaagctg
    62281 aagaggactt ttcgttggtg aacgaggatt attcattaaa cggacaaaac atgatcattg
    62341 cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac tggggattct
    62401 ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta gtcgtcatgg
    62461 tcttgcttgg attcgaagta ctgatgatat taacgaagag gaaaaaagct ctatcgctaa
    62521 tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg caacatgtgg
    62581 gcgatttgat tttctttact ttgggactga tgattgcttt ttatcattat ttctacaatc
    62641 atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc ggtattgggt
    62701 ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg attttttatt
    62761 ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg gatgtagtgt
    62821 tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg ctaacgtcaa
    62881 aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta agtactggag
    62941 gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt ccttttgaag
    63001 atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt ccaactgtat
    63061 tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag ttactgggac
    63121 gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg ttgccaaaat
    63181 ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc cttactttta
    63241 gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga gaagatgtta
    63301 ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat ttctatgcga
    63361 ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca gttgctgtgt
    63421 tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt tacctcgctt
    63481 ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa ggtacaggag
    63541 cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa gatcctggta
    63601 aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga gtgaaatcct
    63661 tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat ccgaaagaag
    63721 aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct aaggaagata
    63781 caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt gaagacgcag
    63841 aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac aaccaattat
    63901 atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg atttttaaag
    63961 tagaatatcc ggattatcca gatgtaccac aaacacaaac tcagggaaat cagctagtcg
    64021 tacagtaata ggctagctga gaataatcct gaaatattag tagaaaggag agtgaagata
    64081 attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt gcttttgttg
    64141 ctgacgctag tcttttttgt ttggacagca ggggtaggtt ttccagcaac tttgtttata
    64201 tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat tttaaatgtt
    64261 tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct tttcattttc
    64321 ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact gtttcctttg
    64381 attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga tcgcggtatt
    64441 tatctgattg tctttacggt cttcctcgga atcacgttat atcgtgtatt tacagagatc
    64501 caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga tatttggatc
    64561 aatacgaata caatcggaag ttctttgatg actcttgctt tattgatcag tggatttgcc
    64621 agttcttttg aacgatggta tgttcgaatc atcggtggga tagcagtcgt tgcagccttt
    64681 ttaggtattt gggtctgccg atctagaggt gcattagtcg cgttgattgt ttttgcgata
    64741 ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat tgcctacacg
    64801 gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga aaaagtcaat
    64861 ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg agaaaaatct
    64921 gaacaagttt tactaggaat gaaaacattt atcttccagc gaggaaacca atttttagga
    64981 aaccacaata gttataattc tattttgaat atctatggat tgataggatt tgggattgct
    65041 gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt atccaacggg
    65101 cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat ggaggataca
    65161 ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc cgttcatcga
    65221 tatgatgaac cagaagttac ggaggaattc tatgttatgg aagaggcata tgacgatatg
    65281 acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc caggatccaa
    65341 cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc agcctttttt
    65401 ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa aatcttgtat
    65461 catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg gtaaaatggc
    65521 acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa gatttgtatt
    65581 tatttatgaa ggacatttat cagaacagaa aattactgct acagttttcg atcaatgact
    65641 tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc aatccgctat
    65701 ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa atgatcgatg
    65761 caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc ttcttctcag
    65821 atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta aagaaagtcg
    65881 tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac acccatttat
    65941 tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct tcagcaatgt
    66001 ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga ttgacttggc
    66061 tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg atcgtgctgc
    66121 agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat tcaggtcttg
    66181 taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt gagtcatttt
    66241 taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg gttttcacgt
    66301 tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat ttttcagatg
    66361 tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata tcacaaaatc
    66421 ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc cttttaagaa
    66481 atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa aaaaaggtga
    66541 gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga aaatcattac
    66601 gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct cagctttgct
    66661 agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct atttaaatgg
    66721 aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata tcattgaatt
    66781 tgctgacatc ggcgaccata tttatcaacc tgtaaaaacc tattcaagtg ggatgttcgt
    66841 tcgtctagct tttgctgtag cgatcaatgt gaatcctgat attttgattg tggatgaagc
    66901 attggcagta ggagatcttg aatttcaact aaaatgtatg gaaaaattta cagaaatcaa
    66961 gaactcggga aagacgattt tattcgtttc tcatgatatt agttcgattc gccgtttttg
    67021 tgatcgttcc tattggttga aaaatggaaa agttgtcgaa tatggtgata cgatggaagt
    67081 aacgacgaat tacgaaaact ttttgaagaa aaaatcagtt aaaacagtag atcgaaataa
    67141 aaaccaagta gaacgcttcg cagctccaga tatcgtagac gtgacgaaag ccgaattgtt
    67201 ggataaagat aaaaatccag tggatatgct ggaacaagga gaaaaactat acgttaaagt
    67261 aacctatgaa gtaaaagacg atacaatcaa aaatccagtt ttaggaattg ctctgcgtac
    67321 agtcgacaat ttttatgtgt gcggactcaa tactctacta gatggtatca agatcccttg
    67381 gaaaaaaggt gagaatatct tttatctgga gtatgaaaag ttaggtttgt tagctggtga
    67441 atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg tatacaagac
    67501 aaaatacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg tggtcttaga
    67561 ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg gacctagatg
    67621 aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa gttttagagt
    67681 ttggtcctgg aaacggccga ttaacgaaac atttgatcgg agcaaaacaa tgtgaagtga
    67741 gcattgtcga gctagataag gaattgttcg attttgtcag cgagtttgcc caagatggat
    67801 tttatggaga tatcgaaagt tttgaatggg caaactatta tgctggacaa acatttgatt
    67861 acgttttgtt tgccgatgtt ttagaacatt tggtagatcc tggaaaaacg ttgaagaaag
    67921 ttagagaatt tttgaatgaa gaaggagaaa ttctaattac attccctaac cttgcgcata
    67981 attctgtcat gattgatttg ttcaataatc aattgccatg ggcttcttat ggtttgctag
    68041 atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt gaaaaagcag
    68101 gattatacat caatattgaa gattatttgt acttagctgt aggtgataca gaattaaaat
    68161 caacttatga agaattgccg gaagctgtac gttatgattt caaaatgcga ccatttggcg
    68221 aagtctacca atacttcttc tcattgatga aacatccagt agcacaaagt tccattgcgc
    68281 agccgcaaaa ttctaattat gtgaaagtat tagaagtaat ccagcaaacg aaacaagatg
    68341 aaataattca acaaatacca ttcaataatt tcacagggga aaatgagaca ctaagtttcc
    68401 atgtatcaga attagcagaa cgaatggtgt ttcgcttcgc gcagcaacct agctttatcg
    68461 aattcagcgc agaagctgct ggagaaaaac tcacatttat cgattcaaat gctgtagtca
    68521 aaacagtaaa tgattgttat ttgtttgatg gtaaagattt accagagttc tttttaacgg
    68581 atattgcagg gaaagaagtg actatccatt gtcactatcg ttttatcgga gaattgacac
    68641 caacaatgaa agaattgctg gaaacaatcc gtccaatggc tgaagttaca aagcagcttt
    68701 ctgagaaaaa tgatgaatta gaaagagaaa atcattatct tattgagaat aatacaagat
    68761 tagatcagac gttgaaaaca accataaatc gctattgcac gttgttggaa agtgatgaat
    68821 ggacaatcaa acatagatta gggcgacgta agaaagaaac atcgaaaaaa atccaagaaa
    68881 aagagctttc tctatgtatt gacgaaaaaa tctgggatgc agaaacaaag atattgaaaa
    68941 tcatcgggtg gggaatcgct aattcggatc gccaaccatt gtcctataag ttatcagccg
    69001 accaatcacc attttttgaa gcaattcctg ttttacgaga agaagtcaat caagcaaaac
    69061 agctagctga aggagcaaaa gcaggatttg aactgcggat cctttgcgag aaggaacgtt
    69121 cattcttagt tgaagcagtg gctcaaaatg gtcaatcatg gatcattgaa atgtaaataa
    69181 tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa ttgccgtatt
    69241 tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg gctgggcaat
    69301 tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg tgattgctga
    69361 agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg ttaagaccca
    69421 aagcggtttt aaggtacgtt tatcagggaa aatgagagga aaagcaatct tagacttcca
    69481 aacagcaaga catcaaaatg gcatagctgt gaaattaaac ggtaaatatc cttatgatga
    69541 cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga taaattatgc
    69601 aaggactcat ggcgtaaaaa aagcaatccg ccgggtcaaa ctggaattga aaccaggatc
    69661 gatcgactat gcagagtgga tcagtcgtca tgagaaacct gatctgaaag agcaaaggaa
    69721 agaagtacaa ggatttgcct accgcccatt gatttccata cttatgcctg tctataacgt
    69781 tgaaatcaaa tggctcgaaa aatgcattga ttcggtactt gatcaaacgt atgatcactg
    69841 ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt gtctagaatc
    69901 ctatcaagca aaagatgata gaattaaagt cgttttccga caagaaaatg gacacatcag
    69961 cctggcaact aattctgcat tggaaatggc agaaggagag tttatagcgc tcttagataa
    70021 tgatgacgaa ttacctccat ttgccttata tgaagtggca aaagtgctaa acgtacatcc
    70081 agaactggat ttgatctaca gtgacgaaga taaaatcgat gcagatggga atcgctttga
    70141 cccgcacttc aaagcagact ggtctcccga tacattgatg ggaaataatt atatctctca
    70201 cttaggcgta tatcggacaa gcattgttaa ggaacttggc ggtttccgta aaggatatga
    70261 aggctctcaa gattatgatt tggttcttcg tgtgacagaa caaattcctg cagaacacat
    70321 ttaccacatt gaccgtgtct tgtatcattg gcggacgatc ccgggatcaa ctgcaagcaa
    70381 tggagaagct aaaagctaca tctatgattc tggagtgaaa gcattgacag atgctctatc
    70441 tagaagaaat atcaagggaa gcgtacatcc tggcagaatt tctggttttt atgaaatcac
    70501 ttatgatgtt ttgcaagaag acttggtcag tgtgatcatt ccaactaaga atggctatga
    70561 agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttatccaa attatgaaat
    70621 cattattgca gataatggaa gtacagatcc taaaatgcaa gaattatttg cagaatacaa
    70681 gcatcagctg aaagaccgat ttatcgtcga attaattgac attccattca attattcacg
    70741 tatcaacaat ctggcagcag aaaaagcaaa cggcaaatac tttttattct tgaataacga
    70801 tacagaagtg attgaacctg actggatgac agcgatggtt tcttatgcac agtttgatcg
    70861 aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc atgcaggagt
    70921 attattagga atcggtggtg ttgcagggca tgcattgaat aactatgatc gaacacattg
    70981 tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg cagcttgtat
    71041 gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat tagaagtagc
    71101 attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca atgtctatgc
    71161 acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag atactccaga
    71221 aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc cagcatatat
    71281 tgcgcatgat ccattttaca atgataattt aacaaaagaa ggaatcggcg acttttcgtt
    71341 gagaccagat taaactataa taaaaagctg ttatgttgtg attcgctcgc acataacggc
    71401 tttttatagt tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa taagtcgagg
    71461 ttgggacaga agtgtttaac tccgagaaat aagaagaaat tcacgaaaat tgcttttcaa
    71521 atttttgtga atttcggctt atttaagaag aagttgcttc tgctcccacc gtttattcgt
    71581 ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc acgctcttat tcatgctcag
    71641 agcgccaata gatacaaaat catagccaaa gtttcacgaa ccctagctgt caaagaatcg
    71701 atattttatt ttaatatagg ctattttaaa ataaatggtt taaggtattc ctctacaaaa
    71761 gccactctat caattatcat gggtttaaaa taagaagtga tggagataac cagattgttc
    71821 ttagcgtcaa tataaagaac atttccacta ttaccaattg ctgaatatat ttcgttctca
    71881 gaatctaata cccaccataa atagccatat ctcatatttt gtaagtcttc taaagcactg
    71941 gttctaggtt tgaacatctc gtttatccac atgggtggta cgatttgttt tcctttatac
    72001 atccctttat ttaggcataa tacaccaatt tttgccagat cacttgccga taaacacaaa
    72061 ccatatcctg ccgtcccgat atttagtgga tcacaaaacc agacattggt tttaggagac
    72121 ttatccattg taaactgctt atgttcttca gctgtttcag cataataatt gacatggttt
    72181 ggtatcccta taggatcaaa tagatactta ttggcaaaat caacagttgt tagcttactt
    72241 gtctgataca acaagccagt taatatgtgt aaacagacag tttgatagtt gaattcgccg
    72301 gttatacctt ttcttcctcc caataaatct agtgatgttt ttgtccaatc ttcacttgaa
    72361 cagacttttg tccatggatc gcctttacat ttgtagggtg ctttcattgt tagcaagtgc
    72421 ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca ctttatactc tggaaaataa
    72481 tctaatactt tatcatcgat acttttgatt aatccttgct ctaaagcaat gccaatcagc
    72541 aaagatacaa tacttttcgt tgctgacatg atatgacaag tatcgtcttt tttataacca
    72601 ttccattcat ctgtataaac ttctacccca tccttatatg cgacaatttg acatatattt
    72661 ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat tcataagata ttacctcact
    72721 ttcttctata agcataatgt cattaaaaat cgatttcaat tttttcgatt cttcacgtaa
    72781 tacaggtatt cttatggact agacagcgct ttgggaagat ggattctctg aaaaatataa
    72841 aggttcactt tatgagtgga taaaaaaagc agcaaatact tcgacttctt taatttacca
    72901 acattagtga aaatcgattc atctggagat ttgtaattta gagtccttgt cttgattaga
    72961 ggtgtataaa gctttttatt agacattttt aaattccatt acaatttgct tataaactag
    73021 gtttttaaag gtgactatgg taagataata gagatattat tttgtgatta ggagtgtata
    73081 tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat taatggatgt
    73141 tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg gagagatccc
    73201 attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga tttttattgc
    73261 gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg acatcgtgtt
    73321 tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga cttttgcggg
    73381 ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg gtacgctttt
    73441 cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc gagataaaaa
    73501 ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt ttggttcaaa
    73561 gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg caaacatcaa
    73621 aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag aaactgaaaa
    73681 aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga atacaacatt
    73741 tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa gtattattga
    73801 agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt ttgatatcat
    73861 cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag caattttagt
    73921 caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa aaaatcaaaa
    73981 ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa aatctggacc
    74041 agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta ttcgagctgt
    74101 ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt cgatcgtagg
    74161 accacgtcct gaacgtcctt tcttcgtcga tcagttcaat gcagaaaacc cttattacta
    74221 cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg gaaaatatgt
    74281 ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat attcattgat
    74341 gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa tgtcctctca
    74401 aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc cgaatgaaaa
    74461 aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca taatcgtacc
    74521 agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag aattggatta
    74581 tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac aggaaatcat
    74641 tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca atcaagggcc
    74701 cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct tattcttcga
    74761 tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc tagaaaagag
    74821 tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg agggaagaat
    74881 cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg aagagtttat
    74941 aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga acaaatgcta
    75001 tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata gttgtgagga
    75061 tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga acccaaaagt
    75121 agaatatatt tatgaatttg aaggtgggaa ggggatcact aatcaatacc gacctaacaa
    75181 gttcgaaaca tttaaagaat tctattcatt agcaaatact gtaacagacg atattaataa
    75241 agacggtatg gcggcgcttc ttttaaaagg gacaacttca gttatttttt ctatttatag
    75301 tacctctgca agaacttcaa aagagaagaa agacgaagca aagcagatat tgagtgatcc
    75361 agtgatccga gaagccaagc aaattgctaa gacggattct tcagcaaaaa aaataacaaa
    75421 gttgctttac aatatgccag ctccactatt tttatctgct gtaaaagttg gaagcttcgt
    75481 tgaagtgaaa atgccagggt taatggctgc tttgaagcga gtatattagt taatggagga
    75541 aaatataatg aaaagaattt tgcattttca gggacgaatg ggtttaggtg gagcagagtc
    75601 tttcatgatg aatttatatc gtaaaattga tcgtacaaac tatcaatttg attttttgat
    75661 ttatgaagat tatgcagatg ttcaagacta tcattcagag attgaaagat taggaggccg
    75721 tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa tatttaatag aagtgaataa
    75781 gttactaaaa aaagaatctt tttctatagt tcacaatcag gtttattttg gcgggggaat
    75841 taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata gccatgcaac
    75901 tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt taaccaaatt
    75961 gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg aaagtctgtt
    76021 tcaaaaccat ccctttgaga tagttcataa tggtattgat ttagagcttt atagtaaaaa
    76081 ttcagaagct aaggttaata agagaaaaga attggatatc tcaatgagta cttttgttgt
    76141 gggaaacatt ggacgattag agaaacaaaa aaatcaatct tatttgttag agattttttt
    76201 agaaatacta aaaatgaatt ctgattctta cttgatttta gtaggtgaag ggtcacttag
    76261 aaataaactg aaagccaaag cagtagaatt ggatatagag aaaagagtaa aatttttagg
    76321 agaaagaaac gatattcccg agttattatc tgtgatggat gtttttgtaa tgccttctct
    76381 ttatgaaggt ctgccaatgg ttggattaga agcacaagca gcaaaattaa aattagtctt
    76441 agcagatact atttccaaag atacaaagtt aacaaataat gtggattttg tatcattaga
    76501 tgaaacacca caagaatggg caaaaaaagt aataaaagag ccgtatgcta ataaaataac
    76561 acaagaacta aagagttatg atgtttcgta cactactgaa caaatggaga gtatatatag
    76621 tagagatcat taaaaatgtt caagatattt taggagtgat tatttttgaa aatatgttat
    76681 ttgatattgg catacaatga tattggcaac ttaaagagat tggtcaatcg attgaacgaa
    76741 aatgcagttt ttattattca tatagataaa aaaactgata tagaaccatt tattgaagca
    76801 tttaaatcat atgataatgt tgaattttta tgtaataaaa accgtgttaa aatttattgg
    76861 ggtggttttt ccattgtaca agcagaaatc aatttagtaa agagagcatt acaaaatgag
    76921 aaatatttaa aatatgtatt attatctggt gctgattatc caatcaaaga taatgagtat
    76981 atttataatt attttaaaaa gaatagttca gtggagttta ttcgtggaat agatctagat
    77041 caaattaaac ataaagaatt ttattataaa catatagatg tatatcaaaa acatgattat
    77101 cctagaatta ataggaataa cacaactgca tttaaaattt ttagagctat aattaatcgt
    77161 tgtctgagga tgattaaact tcccccaaaa attagacacc ataaatttga tttgtatcat
    77221 ggttctcaat ggtgggcttt gtcgaaagaa tgcctaacag aattaataca aatgtatgaa
    77281 caaaatcaac aagactacct gaattttaaa ataggtatgt ttgccccaga tgaaaaattt
    77341 tttcatacac ttttttttaa ttcctcgttc aaaaataaaa atgttattgg aggaccagat
    77401 ttaccacttg aattgaaaaa tattgaagaa actactttac aaacttcgaa attagcaaat
    77461 attcatatta ttgatccttc catgaacaaa tggtttgatg aaagggatat agatcaaata
    77521 aaagaaagtg aaaaattatt tgtaagaaaa gttaaatcgg attactctga taaactatta
    77581 aatagaattg ataaagaaat tttgaaagtt ggaaatatag atgaatagaa tagtacctgt
    77641 tttagagaag agtttattaa gtcgagatgc gtttcgagta ataaatttaa tcatttgtct
    77701 gctttatttt atgcctagta cttttaatgt aactaatatc ccgatgaaaa tagcttttgc
    77761 ttggggtgga attatagttt tgtatgaggt ttttataaag aaaaattttt ttaaaatgcg
    77821 tttttcttgg ctactgattg gagcaatgat tagttttatc ttaactataa tcattaacag
    77881 agaaaatttt ttaattccat ctatttataa ttttggatat ttgctgatta ctttgatagt
    77941 aatttttcct gcagactttt cacaaagtga agaggaaaaa aagaaaaaaa tggtgtattt
    78001 taataatgtt ttcattatca taattttttt agctgcgcta atatctatct atcaatttat
    78061 ttttttgatt tcttatcatg tcccaacagg aacgccagga ttacttgcaa gacaaggttt
    78121 tattgaacat cggttatttg gtgtctatac aagtccaaat gttggagcta tgtttggata
    78181 tacttctatt attttaagtt taattagtgt attaatttca aaaataacaa aaacgattcg
    78241 tgttttttat atgatgaatt ttatcgtaca aatattttat attacacttt cttcgtctag
    78301 aggtgcccag gtagtaattt gttctttttt agtattattt cttttgttgt ttgccaattc
    78361 aaaatttaga aagagcatta gagaagtact acctgtaaaa atcaaatgga tggtaagcat
    78421 attattagtt ctattggtat atttttcttt tggtactaat tatgctaagg attttttagc
    78481 aacaattcct gtatcgattg atagaaaagt agaagttata gagtcaaatg aatcagataa
    78541 aactccactt ccagaaaaag aagtaatatt acagcattct agtgataact ccgaaatttc
    78601 agctggtcgt tttacgattt ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt
    78661 tggttatgga gagaccgatt tctatgggaa tagcgatgca aaatttttga aaaaagtaga
    78721 tcttgatact acagatgtga atgaactaaa aagagcacat ggaaacatgc ataatggatt
    78781 tcttcaagta cttgtatcaa gtggtataat tgcttttata tgtatctatg ctttctatgt
    78841 gttaaatatc tttagtttac tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct
    78901 tggtattgtt ttaattttta tactatctat ttttatagat gaaatggtgg aggctcatgt
    78961 acttttcaat aagcgcgatg tcatttcaat tgttttctgg tattatttgg gaagcatttc
    79021 ttacttgtat ttaaaaaagg agaattaata tgaagacagc tttcattgct tggactcctt
    79081 tacatttgat taatatatta aatgtttatc aaaattttta ccgagaatca aaaggagatt
    79141 tatttattta ttctgaattt gaaaacgcta gaagatatag tgagcgtata aaagaattag
    79201 ggctatttaa tgatgttgtt tttgtgaatc accggaaaat cggttcttcc tttaaacgta
    79261 aaattaactt gttaactaac cataatcaat ttttctcgga tgatttatcc gtttatgatg
    79321 aaatttttat acagggggaa aattatttca caaaaatact tttctccaat gtttacaaaa
    79381 aaaatccaga tgtaaaacta aattatatag aagatggttt gggtacttat ttgaagaaga
    79441 gtatattttc aacaagtaaa aacgttcaaa gagtttttaa aatattaaat aaaaatagca
    79501 tcttcaaaaa aaaaattgac gattattttg tctattgtcc ggaattgtta ctttatgatg
    79561 taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag taaattgatg aaaaacatac
    79621 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
    79681 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
    79741 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
    79801 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
    79861 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
    79921 tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag
    79981 cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata
    80041 accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg
    80101 tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg
    80161 tatactatag ggaatttttt tattaaggga atttcattca taactattcc tatttttgta
    80221 aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt
    80281 ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat
    80341 aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt
    80401 gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac
    80461 caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc
    80521 cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct
    80581 acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa
    80641 tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat
    80701 ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta
    80761 aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat
    80821 agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt
    80881 aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg
    80941 ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat
    81001 ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa
    81061 tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc
    81121 tatttctttc agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca
    81181 tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt
    81241 ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta
    81301 ctttttattt ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca
    81361 aaaaaaatat gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta
    81421 atcaatcaaa tgttgattcg ttacgttttg acatcattat tgataattag tttagttata
    81481 tattttttaa agattaagaa tcgaattata gatgactata attcatagga ggagatataa
    81541 gtgtttaaag gaaaaacaat attagtaact ggcggaactg gttccattgg aagtgaaatt
    81601 gttcgtcagc ttttaaaata tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa
    81661 aaacaatttt atatgcaaca agagctctca aatcacacta atttgagatt tttaattggt
    81721 gatatcagag actctcaaag gattgattat gcatgtcgtg atgtggatat tatttttcat
    81781 gctgccgcaa tgaaacacgt tccagcaagt gaatataatc ctatggaagc tgtaaaaaca
    81841 aatgtgatgg gaactcaaaa tgtaatagat gcagcgatca aaaataatgt aaaacatttt
    81901 gtaggtatca gtacagataa agtagtgagt cctactaaca ccatgggtgc gacaaagtta
    81961 ttgagtgaaa aattaataat agctgccaat cagtataaag gaaaaacaga aacaatattt
    82021 tcttgtgtaa ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc tctttttagt
    82081 aaacaaataa aaaacaataa agatattact ttgacagatg ttgatatgac acgatttatg
    82141 atgtcaatac cggatgctgc aagattagtt ctgtctgctg ctatgaattc aaaatttgga
    82201 gaaacatatg tcttaaaaat gccggcaatc aatattttaa ctttagctca atcattaata
    82261 aaaatatata atgatacaca tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc
    82321 aggccaggag aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat
    82381 gaaaatgata aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa
    82441 gattatgaaa aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga
    82501 caggaattta ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga
    82561 tgaaatcaga agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt
    82621 tagctttaga aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa
    82681 catttaaaac gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata
    82741 atcttaaaga ggacagttct caatttgaaa tgctaaaaaa attagaatta tctcaatctg
    82801 attttattag aataaaagat tattgtatac aaaataaaat tgattttttg tctactcctt
    82861 ttgatgaaga aagtgcagac tttctaaaac aaattggtgt acaaggattt aaaattggct
    82921 caggagattt aacaaattta ccttttctta gaaaaataga tatgtatggg ttgccagttt
    82981 tattatctac aggtatggct gatattcatg aggtcagtga ggctgtagat tgtttcatag
    83041 atagtccagt tactatttta cattgtactt caaattatcc ggctagttct gaagatatta
    83101 atttattggc gattaacaca ttaaaagaaa tgttcgggat acccgttggg tactccgatc
    83161 attcattggg atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga
    83221 aacattttac cttggataag gaactacctg gtccagatca taaagcttct ttagatcctc
    83281 aggaatttgc agattttgta aagcatatta gaaacactga aatattttta ggtgatggtg
    83341 ttaagcgagc aatgccttct gaacttagta caaaagaagt atcaagaaaa agcctagtgg
    83401 tcactgagaa tttaaatgtg ggagatattc ttacagagaa atctcttaca ataaaacgtc
    83461 ctggaaatgg aatagctcct aagtttttat atcaatatat tggcaaaaaa gttaaaagac
    83521 caataagtgc agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa tacttatagt
    83581 aagtggaaca cgagctgatt atggaatata taaacctgtt gctgagaatt tactacaaga
    83641 taattgggat gtatcatttg ttgtttctgg aatgcattta tcccataaat atggatttac
    83701 taaagaattg atcttagctg acggatttag aattagtgga gaagttcaaa gtttgttttt
    83761 agagaacaat aagggaaaca tggctcggtc tgtggcatta gagataaatg gtttcacaaa
    83821 tatatttgaa caaactagcc ctgattttgt attattacta ggggatagag gagaaatgtt
    83881 ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca gcacatattc acggaggcga
    83941 agtgtctggt actgtagatg aatcaataag acatgccata acgaaaatag catctgttca
    84001 ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa atgggggaag attcttggag
    84061 agtttttccc gtcggagctc ctagaataga tactatttta aataatagat tacctagttt
    84121 tagagatata aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg ttttgcaagt
    84181 atttcatcca gttgtaaccg aactagaaaa tatagaaaaa caaattttag ctttgatcaa
    84241 ttcaatggaa caacaatctc aaccaattat ttgcatttta cctaatagtg atgctggtag
    84301 cgaaattatc agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa
    84361 tttagagccg gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc
    84421 ttcttctggg ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag
    84481 acaaaatgga cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat
    84541 tgaaactgct ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa
    84601 gaacatctac ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt
    84661 gaatgatggg gaagatatta agtggattca aaaacaaata gcttattaaa atatactagt
    84721 gatccataca ttattattgg ggctggcgga cacgcaaaag tcattattga aaccttaaga
    84781 ttagaaaata agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg
    84841 aaatatcttg ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat
    84901 aaatttattg tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat
    84961 atgccaagtg agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt
    85021 tcaattggtt atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt
    85081 ggagagcatg ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac
    85141 tatgttcatt tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact
    85201 caaataggaa ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata
    85261 ggcgcaggga gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca
    85321 gctaaaatta agaaagaagg aattaatttt gaattctaga attttattat caagtcctca
    85381 tatgggagga acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc
    85441 attaggtgaa aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg
    85501 cggagcttct gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa
    85561 tgttggaaaa aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc
    85621 tattttatat caaggggctg taccagtgtt cattgactca gaacctaata catggaatat
    85681 gagccctgtt gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa
    85741 ggcaatcatc gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat
    85801 tgcaaaccaa tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta
    85861 taagggaatt tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa
    85921 gatcatcaca acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca
    85981 ttcaagattt ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat
    86041 aggttataat taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt
    86101 attgaatgat agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc
    86161 tggaattgat agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt
    86221 gtctacttta acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa
    86281 actaaatgaa aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt
    86341 atttcaaggg gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt
    86401 taatagcggc ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt
    86461 tattgatgag tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat
    86521 tggtttaggt tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat
    86581 tttactatgt ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg
    86641 aataccaact tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt
    86701 tgtttgtaca tcccctataa cccatgaatt tattataaaa gaggctttaa atttagattg
    86761 taatatcttt acggaaatca atttattaaa tgaatattat gatgaaataa tcgaagtagc
    86821 taaaaagaaa gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca
    86881 atatattaag tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca
    86941 gtatttaccc gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa
    87001 aactaatggg tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg
    87061 gaaagttaaa tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc
    87121 ggacagtttc actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga
    87181 tgttgtttca agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga
    87241 atgggaaggt aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga
    87301 tattcaattg tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga
    87361 tgcatattta gaggagatta aagaattttt cacgcttttg gaaaagaaaa tggaacccca
    87421 atatactttt gaaaaagata agtatattat taacttaatt aacgacattg aggtaaatta
    87481 atgatgaaag taattttttt tgggttaggg tcaattggaa agagacatct aaaaaatcta
    87541 ataagctatg gaaaaaagaa ttctattaat tttgatataa cagcttatag tagcagtgga
    87601 aaaaaagagt ttgaagaaat aacgtatatt caaaatagag acgatttgga tacacattat
    87661 gatatagctt ttattacaaa tccgactttt aagcatctgg aaacactaaa tgtaattgtt
    87721 gaaaaagttg attacatatt tttagaaaaa cctgcttttt ctgagagtat tgatcttact
    87781 caatataatt atgattacaa aaatatttat gttgctgctc cactgaggta taaaaatatc
    87841 atggaagttg ttcgtaatag tattcaaagt aaaaaagtat attctgtaag agctatttgt
    87901 tctacttatc ttcctgattg gagaaaaggt actgattata gaaataatta ttctgccttt
    87961 aaatctatgg ggggaggagt agaattagac tgtatacatg aattggatta tattataaac
    88021 ctatttggtt tccctaaatc agtaaaaaaa cagtttggta aatattcgaa tcttgaaatt
    88081 aacagtaatg atttagcaac atatatattg agatacgatg ataaaattat agaaattcat
    88141 ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt agatgaatgt
    88201 atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt taaagaaatg
    88261 cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga aaacgtagta
    88321 acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa aatagctgag
    88381 gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa ggggtttaaa
    88441 aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt agcaatgata
    88501 gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa tacggacagt
    88561 aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt agaacgtaaa
    88621 cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc attcttacaa
    88681 ctagaaagag aagataatat atttgattta attatagatt tagatttgac ttcaccttta
    88741 cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac
    88801 gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt aaaaagaatt
    88861 gaaaatgaag ttactatagt taataaatca agatacacat ctaggcaaca gacacctgaa
    88921 gtttttgaaa tgaatgcctc aatgtattct tttaaacctg aatttctaaa aaaaagtaat
    88981 tatatttttg atgggcaatg tgggataata aaaatgagag attatctaat tttagatatt
    89041 gatagtgaag aagattttaa atggttagaa tttttgtttc ctaaattttt agaagattct
    89101 tcggagcttc aagatatcta taattattta tatcggaggt aaaaggatga gctgttatct
    89161 tatcaccggt ggggctggct tcattggttc taccttagcc aatcatttag gagaagaaca
    89221 tgtggtaatc gtcgtcgatg acttatcaat gggaaaagta gaaaatcttg atatgacgaa
    89281 aaatatcact tttattgagg gagatgtcgc tgagaaatcc ttgatggaag agatcatgaa
    89341 agctaataaa tttgattata tctttcattt agcagcagtt gctagtgtgg cagattcagt
    89401 tgcccgacca gtagaaacac atcgtgtgaa ttttgaaagt gtattaatga ttttagaatt
    89461 gatccgcaag tatcaacctg atttaaaacg gatcgtgttt agttcttcag cggctgttta
    89521 tggggatgaa ccgactttac caaaaaaaga agagtccgtg attcgcccat tgacgcctta
    89581 tgcggttgat aaattcgctg cagaacaata tgtgctagat tattgtcatt tatatgatgt
    89641 acccggtagc gcagttcgtt ttttcaatgt ctatggtcca aatcaaaatc cgaattctcc
    89701 atacagtggt gtgatttcta ttctcgtaga tcgctataag aaacaaatcg taggagaagc
    89761 aacttcattt acattatttg gtgatggaag ccagtctaga gactttgttt atatagatga
    89821 tgtaatccaa gcattacttt tagttgcgaa tgaggaagct gcactgggga aacaattcaa
    89881 tgttggaacg ggtaaagcaa ccacactttt ggaattgatt catgcaatca atgaaattct
    89941 cggtgtagaa ttgacacttg aatacaaaga agagcgttcg ggtgatatcc atgactctct
    90001 agcagatatt actaagatcc agtcaattgg ctatcaaccg aaatatggta ttttaagtgg
    90061 aatgactaat tatttgaaaa ctgaaatcaa ttaaaacata ctaagataag attagagggt
    90121 aaattcaggc tctttgtcaa taaggactga tgagttttgt aaaatcaaat ctgggataga
    90181 a
//
LOCUS       DACEDD010000004        86094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEDD010000004 DACEDD010000000
VERSION     DACEDD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.004"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1165..1563)
                     /locus_tag="IWO45_000266"
     CDS             complement(1165..1563)
                     /locus_tag="IWO45_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331775.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKYT
                     SMKPCILDWQWLIDAVLDVKCSKYNITLGC"
     gene            1779..2834
                     /locus_tag="IWO45_000267"
     CDS             1779..2834
                     /locus_tag="IWO45_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ0331776.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMKQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLSISPNAFQLEKIIREGKEKGIYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFSTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2854..3207
                     /locus_tag="IWO45_000268"
     CDS             2854..3207
                     /locus_tag="IWO45_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331777.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHIPIQQEHPSTVTSDHLAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3402..3710)
                     /locus_tag="IWO45_000269"
     CDS             complement(3402..3710)
                     /locus_tag="IWO45_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331778.1"
                     /translation="MIEVKENRFKKFADAEIEEVPEVVEAVEKPKQKPKQKPKETTSS
                     LIAKLKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYAEVFGE
                     "
     gene            complement(3714..4532)
                     /locus_tag="IWO45_000270"
     CDS             complement(3714..4532)
                     /locus_tag="IWO45_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0331779.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKSENTINHLYLHGSAEPLAINESMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVINYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALSKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKDTYRKNKNFFESTFELYNKIIDTAQE"
     gene            4748..4969
                     /locus_tag="IWO45_000271"
     CDS             4748..4969
                     /locus_tag="IWO45_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ0331780.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEESDQSISHEDMLKELGL"
     gene            4971..5237
                     /locus_tag="IWO45_000272"
     CDS             4971..5237
                     /locus_tag="IWO45_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0331781.1"
                     /translation="MTYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5402..5686
                     /locus_tag="IWO45_000273"
     CDS             5402..5686
                     /locus_tag="IWO45_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331782.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLENVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            5693..5938
                     /locus_tag="IWO45_000274"
     CDS             5693..5938
                     /locus_tag="IWO45_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331783.1"
                     /translation="MRTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            5960..6214
                     /locus_tag="IWO45_000275"
     CDS             5960..6214
                     /locus_tag="IWO45_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331784.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISSKDLENALAYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKINKSFY"
     gene            6296..6481
                     /locus_tag="IWO45_000276"
     CDS             6296..6481
                     /locus_tag="IWO45_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ0331785.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNFKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6548..6943
                     /locus_tag="IWO45_000277"
     CDS             6548..6943
                     /locus_tag="IWO45_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ0331786.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIIFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            6933..7310
                     /locus_tag="IWO45_000278"
     CDS             6933..7310
                     /locus_tag="IWO45_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331787.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHLANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            7311..7706
                     /locus_tag="IWO45_000279"
     CDS             7311..7706
                     /locus_tag="IWO45_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331788.1"
                     /translation="MKQEKRKMKQTQTETINDTLADQLAGYYKLFEMSKAQRLIHFLW
                     KYKILFLFWEATLTFSILFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEENLVIS
                     DAGIERNIKRVSIKITLNKPTDKKLSEAF"
     gene            complement(8012..8410)
                     /locus_tag="IWO45_000280"
     CDS             complement(8012..8410)
                     /locus_tag="IWO45_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ0331789.1"
                     /translation="MKYLFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(8601..9368)
                     /locus_tag="IWO45_000281"
     CDS             complement(8601..9368)
                     /locus_tag="IWO45_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0331790.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLEIELKTIVNTLQRQAVYEISNLEKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(9404..10627)
                     /locus_tag="IWO45_000282"
     CDS             complement(9404..10627)
                     /locus_tag="IWO45_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0331791.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIAELRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TDIKFLLTRTMSVPVPYKQEQRDLAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKFMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            11585..12388
                     /locus_tag="IWO45_000283"
     CDS             11585..12388
                     /locus_tag="IWO45_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0331792.1"
                     /translation="MSQEVTEKVIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            12971..13426
                     /locus_tag="IWO45_000284"
     CDS             12971..13426
                     /locus_tag="IWO45_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331793.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEDIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            13545..14603
                     /locus_tag="IWO45_000285"
     CDS             13545..14603
                     /locus_tag="IWO45_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ0331794.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            14645..16594
                     /locus_tag="IWO45_000286"
     CDS             14645..16594
                     /locus_tag="IWO45_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0331795.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKKRVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            16597..20238
                     /locus_tag="IWO45_000287"
     CDS             16597..20238
                     /locus_tag="IWO45_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331796.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFMRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGKDVSDRVYRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNENNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            20638..20976
                     /locus_tag="IWO45_000288"
     CDS             20638..20976
                     /locus_tag="IWO45_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331797.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            21086..21328
                     /locus_tag="IWO45_000289"
     CDS             21086..21328
                     /locus_tag="IWO45_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331798.1"
                     /translation="MVFDREKMLAHANEVLMSGLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIRFEKAYVYMLDKQKRTIDRKKGVLQ"
     gene            21328..23484
                     /locus_tag="IWO45_000290"
     CDS             21328..23484
                     /locus_tag="IWO45_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331799.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLIKDLPKKNYMVIEDVQKLNSEEVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLLIPDDPNMFESEVEEATDFYSAVEQLEVATN
                     GLERLAETFNSSEGVQFEAVAEDVPDEKEALFVYDDKPTEAENQEVSIEETQIQTENY
                     QQPKIDEKPEFQSDKENSVYEFEKIVSESDIPQNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEDATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            23496..23729
                     /locus_tag="IWO45_000291"
     CDS             23496..23729
                     /locus_tag="IWO45_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0331800.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            23719..24585
                     /locus_tag="IWO45_000292"
     CDS             23719..24585
                     /locus_tag="IWO45_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331801.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            24600..26570
                     /locus_tag="IWO45_000293"
     CDS             24600..26570
                     /locus_tag="IWO45_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331802.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRLEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAEINVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            26737..26964
                     /locus_tag="IWO45_000294"
     CDS             26737..26964
                     /locus_tag="IWO45_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331803.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            26961..27455
                     /locus_tag="IWO45_000295"
     CDS             26961..27455
                     /locus_tag="IWO45_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331804.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFEL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            27630..27965
                     /locus_tag="IWO45_000296"
     CDS             27630..27965
                     /locus_tag="IWO45_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331805.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(28222..29805)
                     /locus_tag="IWO45_000297"
     CDS             complement(28222..29805)
                     /locus_tag="IWO45_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331806.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDITSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLPYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLKILKEEISKYRFTAESREEINKSLGENQQITYTK
                     GVFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGSN
                     NEELQNHLNSLLDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKNLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            29927..30511
                     /locus_tag="IWO45_000298"
     CDS             29927..30511
                     /locus_tag="IWO45_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331807.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSIT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIDTSIKELREYVQNPYNFQYLAGRINLV"
     gene            30522..30767
                     /locus_tag="IWO45_000299"
     CDS             30522..30767
                     /locus_tag="IWO45_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331808.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLVLNKRKKDR"
     gene            30773..31495
                     /locus_tag="IWO45_000300"
     CDS             30773..31495
                     /locus_tag="IWO45_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331809.1"
                     /translation="MKSTKWKIVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            31626..32369
                     /locus_tag="IWO45_000301"
     CDS             31626..32369
                     /locus_tag="IWO45_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331810.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            32375..32740
                     /locus_tag="IWO45_000302"
     CDS             32375..32740
                     /locus_tag="IWO45_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331811.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            32756..33286
                     /locus_tag="IWO45_000303"
     CDS             32756..33286
                     /locus_tag="IWO45_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331812.1"
                     /translation="MSQNISAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            33448..33816
                     /locus_tag="IWO45_000304"
     CDS             33448..33816
                     /locus_tag="IWO45_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331813.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            34007..34267
                     /locus_tag="IWO45_000305"
     CDS             34007..34267
                     /locus_tag="IWO45_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331814.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            34885..35685
                     /locus_tag="IWO45_000306"
     CDS             34885..35685
                     /locus_tag="IWO45_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331815.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(35835..36991)
                     /locus_tag="IWO45_000307"
     CDS             complement(join(35835..36733,36733..36991))
                     /locus_tag="IWO45_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0331816.1"
                     /translation="MGDKRKSPRKFDEEFKKSIVKLYESGKTQNALSKEYGIALSSIA
                     RWIKQYSEVKIDDDTILTARQIKQLQKRNAQLEEENLILKKANCHIHASLDERLKAIH
                     ILRYEHSIVTLCRVLKVNRSTYYKRFSGKVAPRTKENQHLRKVILEIYTSSQKRLDAA
                     KIRRILLRDYGILISIGRVYRLMKSMNLPKMSTVKPVFKKKKNKVSLTRPNHLKQAFN
                     PPAPNQVWTSDFSYIPIGKKKFAYLCVILDLFSRKVIAWTVGSTIDTNLAIKTLDKAL
                     HSRNLMSPVLFHTDQGSQYTSFEFRKFIENHPIVHSLSKPGYPWDNAVTEAFFKYMKK
                     EELNRRSFHSIEEVRLACFTYIEGFYNIKRPHGTLDMLTPTEKEALYFESL"
     gene            37376..37663
                     /locus_tag="IWO45_000308"
     CDS             37376..37663
                     /locus_tag="IWO45_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331817.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            37754..37993
                     /locus_tag="IWO45_000309"
     CDS             37754..37993
                     /locus_tag="IWO45_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331818.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            38121..38450
                     /locus_tag="IWO45_000310"
     CDS             38121..38450
                     /locus_tag="IWO45_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331819.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            38503..39333
                     /locus_tag="IWO45_000311"
                     /pseudo
     CDS             38503..39333
                     /locus_tag="IWO45_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39348..39665
                     /locus_tag="IWO45_000312"
     CDS             39348..39665
                     /locus_tag="IWO45_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331820.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            39668..39900
                     /locus_tag="IWO45_000313"
                     /pseudo
     CDS             39668..39900
                     /locus_tag="IWO45_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40015..40491)
                     /locus_tag="IWO45_000314"
     CDS             complement(40015..40491)
                     /locus_tag="IWO45_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331821.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            40618..41949
                     /locus_tag="IWO45_000315"
     CDS             40618..41949
                     /locus_tag="IWO45_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0331822.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            41974..42501
                     /locus_tag="IWO45_000316"
     CDS             41974..42501
                     /locus_tag="IWO45_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331823.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            42488..43051
                     /locus_tag="IWO45_000317"
     CDS             42488..43051
                     /locus_tag="IWO45_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331824.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            43469..44206
                     /locus_tag="IWO45_000318"
     CDS             43469..44206
                     /locus_tag="IWO45_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331825.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(44223..44639)
                     /locus_tag="IWO45_000319"
     CDS             complement(44223..44639)
                     /locus_tag="IWO45_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331826.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            45032..46279
                     /locus_tag="IWO45_000320"
     CDS             45032..46279
                     /locus_tag="IWO45_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331827.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSNPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            46549..48939
                     /locus_tag="IWO45_000321"
     CDS             46549..48939
                     /locus_tag="IWO45_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ0331828.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            48988..50823
                     /locus_tag="IWO45_000322"
     CDS             48988..50823
                     /locus_tag="IWO45_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0331829.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            50898..51755
                     /locus_tag="IWO45_000323"
     CDS             50898..51755
                     /locus_tag="IWO45_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0331830.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            51745..52824
                     /locus_tag="IWO45_000324"
     CDS             51745..52824
                     /locus_tag="IWO45_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0331831.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            53163..53963
                     /locus_tag="IWO45_000325"
     CDS             53163..53963
                     /locus_tag="IWO45_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331832.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            54051..54857
                     /locus_tag="IWO45_000326"
     CDS             54051..54857
                     /locus_tag="IWO45_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331833.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            55088..55957
                     /locus_tag="IWO45_000327"
     CDS             55088..55957
                     /locus_tag="IWO45_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0331834.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            55977..56303
                     /locus_tag="IWO45_000328"
     CDS             55977..56303
                     /locus_tag="IWO45_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331835.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            56337..57599
                     /locus_tag="IWO45_000329"
     CDS             56337..57599
                     /locus_tag="IWO45_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331836.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            57679..59055
                     /locus_tag="IWO45_000330"
     CDS             57679..59055
                     /locus_tag="IWO45_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0331837.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLG
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQLISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            59052..59681
                     /locus_tag="IWO45_000331"
     CDS             59052..59681
                     /locus_tag="IWO45_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331838.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            59693..60151
                     /locus_tag="IWO45_000332"
     CDS             59693..60151
                     /locus_tag="IWO45_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331839.1"
                     /translation="MLTILKLYREGFNEKVSIGESIEYRGDKYIIIRILKINKHYFSK
                     SISLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIGDIVFPEKGIAYEV
                     VSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVLEGEKK"
     gene            60148..60627
                     /locus_tag="IWO45_000333"
     CDS             60148..60627
                     /locus_tag="IWO45_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331840.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDTLMRKYYKQGIY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
     gene            60766..61305
                     /locus_tag="IWO45_000334"
     CDS             60766..61305
                     /locus_tag="IWO45_000334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331841.1"
                     /translation="MNKRRILWLIAGMYLCIAVSGFTICRIINQLKNAIVYLGEIGYL
                     EDLKAGLQIMSWILFVLTVALVILTCENFSTIKKQIISNHNQETVKSGLEVDPEEEVT
                     EQAIKEQEEIDKKEIMSIYLSSGESFFHEIDSLKLSSQEIIDRWQNMSLDGELIVEDS
                     EGWSAYKKDEIVAITILFE"
     gene            61956..62009
                     /locus_tag="IWO45_000335"
                     /pseudo
     CDS             61956..62009
                     /locus_tag="IWO45_000335"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            62013..62612
                     /locus_tag="IWO45_000336"
     CDS             62013..62612
                     /locus_tag="IWO45_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0331842.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            62628..63104
                     /locus_tag="IWO45_000337"
     CDS             62628..63104
                     /locus_tag="IWO45_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331843.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            63121..64023
                     /locus_tag="IWO45_000338"
     CDS             63121..64023
                     /locus_tag="IWO45_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331844.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            64096..64287
                     /locus_tag="IWO45_000339"
     CDS             64096..64287
                     /locus_tag="IWO45_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331845.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            64451..65038
                     /locus_tag="IWO45_000340"
     CDS             64451..65038
                     /locus_tag="IWO45_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331846.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            65038..65937
                     /locus_tag="IWO45_000341"
     CDS             65038..65937
                     /locus_tag="IWO45_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ0331847.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            65937..66851
                     /locus_tag="IWO45_000342"
     CDS             65937..66851
                     /locus_tag="IWO45_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331848.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQTGGMPRNTKANISRYIKKAE"
     gene            66903..67118
                     /locus_tag="IWO45_000343"
     CDS             66903..67118
                     /locus_tag="IWO45_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331849.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            67304..67558
                     /locus_tag="IWO45_000344"
     CDS             67304..67558
                     /locus_tag="IWO45_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAQ0331850.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            67737..68780
                     /locus_tag="IWO45_000345"
     CDS             67737..68780
                     /locus_tag="IWO45_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ0331851.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            68953..69177
                     /locus_tag="IWO45_000346"
     CDS             68953..69177
                     /locus_tag="IWO45_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331852.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            69180..69437
                     /locus_tag="IWO45_000347"
     CDS             69180..69437
                     /locus_tag="IWO45_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331853.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            69539..69892
                     /locus_tag="IWO45_000348"
     CDS             69539..69892
                     /locus_tag="IWO45_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331854.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            69945..70739
                     /locus_tag="IWO45_000349"
     CDS             69945..70739
                     /locus_tag="IWO45_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331855.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            70768..71886
                     /locus_tag="IWO45_000350"
     CDS             70768..71886
                     /locus_tag="IWO45_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331856.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            71919..72239
                     /locus_tag="IWO45_000351"
     CDS             71919..72239
                     /locus_tag="IWO45_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331857.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            72494..73386
                     /locus_tag="IWO45_000352"
                     /pseudo
     CDS             72494..73386
                     /locus_tag="IWO45_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            73406..75034
                     /locus_tag="IWO45_000353"
     CDS             73406..75034
                     /locus_tag="IWO45_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331858.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            75085..75453
                     /locus_tag="IWO45_000354"
                     /pseudo
     CDS             75085..75453
                     /locus_tag="IWO45_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            75553..75753
                     /locus_tag="IWO45_000355"
     CDS             75553..75753
                     /locus_tag="IWO45_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331859.1"
                     /translation="MLKRIAWDKDNKTVIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            75887..77113
                     /locus_tag="IWO45_000356"
     CDS             75887..77113
                     /locus_tag="IWO45_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331860.1"
                     /translation="MIFYMFININEVLKMTTITMTKNIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLQSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            77972..79297
                     /locus_tag="IWO45_000357"
     CDS             77972..79297
                     /locus_tag="IWO45_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0331861.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            79290..79637
                     /locus_tag="IWO45_000358"
     CDS             79290..79637
                     /locus_tag="IWO45_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331862.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDGLGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
     gene            79988..80809
                     /locus_tag="IWO45_000359"
     CDS             79988..80809
                     /locus_tag="IWO45_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0331863.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            80829..81389
                     /locus_tag="IWO45_000360"
     CDS             80829..81389
                     /locus_tag="IWO45_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAQ0331864.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGILSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            82213..82980
                     /locus_tag="IWO45_000361"
     CDS             82213..82980
                     /locus_tag="IWO45_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ0331865.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIII
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            83006..83548
                     /locus_tag="IWO45_000362"
     CDS             83006..83548
                     /locus_tag="IWO45_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331866.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGMSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            83549..84115
                     /locus_tag="IWO45_000363"
     CDS             83549..84115
                     /locus_tag="IWO45_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331867.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            84130..84378
                     /locus_tag="IWO45_000364"
     CDS             84130..84378
                     /locus_tag="IWO45_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331868.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            84398..84637
                     /locus_tag="IWO45_000365"
     CDS             84398..84637
                     /locus_tag="IWO45_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331869.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            85008..85289
                     /locus_tag="IWO45_000366"
     CDS             85008..85289
                     /locus_tag="IWO45_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ0331870.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            85279..85620
                     /locus_tag="IWO45_000367"
     CDS             85279..85620
                     /locus_tag="IWO45_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0331871.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
BASE COUNT        32084 a        11881 c        16534 g        25595 t
ORIGIN      
        1 tagggaatag gacttttgtt tcgtgttaag tttctttaca ttactacgca cctttcgtgt
       61 aaaacttctt tattcatacc acgctccatg ttattttata tttccttttt catgttattt
      121 ttagccagaa accgatcaag tttttaccta ttcccaaaat ccatttattc gttatttttt
      181 cctgattaag aataattatt tgatgcttaa attagctaaa aaagcgattt gttactgata
      241 taatcatgat attaaagctg aattaggcag aaagataaaa tgttcgcaaa atcaaagtat
      301 caattatctt gtttttttat aaatttgtaa gtaaaaatct aaaaagtttt cttttaattg
      361 gtatatgtat taaaatcaca ttcaatcact cataaaagta tgcgatttca tttttttgga
      421 tgagattaat atcaataaat aacgatgtaa aaaccattta ttatgctctt ttataaggat
      481 attaattgat ctaatttgaa aaaataattc aaaatttaaa tgttttaata ttacgaataa
      541 aatcatgtta tcgtaaaatg gtataaaaaa gctaaatatt atatttttgt tattttatta
      601 gcagaaaaat ttaaatagtt ttacaagaat ccgtagaaat atattggttt tgccttcaga
      661 ttaatactgg atcatattaa tctgaatata atatatgaac taataaatag cttaacctcg
      721 ctattttgtt ttttctgatc actatttata cagcattagt aaataaaatt ttaaaaataa
      781 aaaaaataag acaaaaaaag tatcaacata aacatataca tatacacgtt tatgttgata
      841 cacttaattt gtcctttaaa aaacactttt tttgatatta tgttatttta taatcttatt
      901 acaagctgat tgttagacgt tgccctttca ttacatttat taacggttat tttatcgttt
      961 aatttattaa ttatttttga tctaaaactt agcttaacaa ataaataatt aaccaaccgc
     1021 cagataaaaa tgcgatcatt gctaatacat aagcaaagat aaaaatatgt tcgatcagct
     1081 taaaccctga tgcgattaag cagaccaagt aaaatagaaa ttctcttaca gataatggta
     1141 attttctaac tagcatactc ataattaaca ccccaaagta atattgtatt ttgaacattt
     1201 tacatccaat acagcatcaa tcaaccattg ccaatccaaa atacaaggct tcattgaagt
     1261 atatttttca gatgaaaaga tataatataa tttcttcgga ataatatttt cagttgttgg
     1321 gatcaagcta aatctaatac cgtctaactc atagctacca gctttctctc cattaaaaat
     1381 ttccttagca ctttcttcta aagaatcaaa atatttttcg acagcaacct ttgaagcttt
     1441 tactattgaa gcatttccct tttcgataaa atctgctttt gttttaccga caataagtaa
     1501 ttcttgatca gtaaaatctt ctaaaataat ggtagtgtaa ttcttatttc ttctcaatcg
     1561 catcaggatc actccttaat ataatttatg tttttataat atcataataa gataaatttg
     1621 caagcattta tttacacctt tttatttaca ctttattata attaattata cttttatgat
     1681 tgcatttttt tcttataaac agttgcaact aatagtaaat aggtgtaaac tattttcgta
     1741 ccgaaatacc ataaacacaa acagaaagag gtcataccat gaaagaacca ttattgtttt
     1801 ccgttgactt agggaataaa tcagtaaaag aaatgagtac acacgtttct ccactctcac
     1861 ttccctctcg tattttgaac aaaaatatca ttaattcagg acgtttttct aatataggtt
     1921 cctcattaaa acgatcacta aatgtttctg aatatagcct agtaaacact actgaaagct
     1981 attattttgg aaaggatata cacgaactag gaaaagatga ctacgtgatc gaatcactag
     2041 gttttggaat cagcagatac aaacaggaaa cctaccaaaa tatcctatct ttctctttag
     2101 ctaacctagc acgtttagaa gatcaatctg atgacgtttt aacagttaat cttgttttag
     2161 gtttgccttc atccgactat accgaaacaa tcgtcaaata cgtgatgaaa caagccacac
     2221 gacaacattc agttgaagtt gatggtaaag cttataatat aaacgtagat accgtttggg
     2281 tactgccaca gccgattgga acactatata accttctact tgctgatgat ggtagtgtac
     2341 aagatgaatc attgatcagt cagaagatcg gttttgttga tattggtggt ggcacgaaac
     2401 tttttgatgt tgtccaaaat tttgaaattg acgaaaacaa tcgtgaacaa aaggataccg
     2461 gcattttcac tttatataat cgtatcgtaa atatgcttga tctttcgatc agtcctaatg
     2521 ctttccaact tgaaaagatt atccgagaag gcaaagaaaa aggtatatat ctatttaagc
     2581 caagcggaaa tgaaagtttt gatatttcag aagtagttga aagagaaata aaaacataca
     2641 caaatatggt tgttgcagaa gttttagcta cattcaaaaa cttctcaacc ttggataaag
     2701 ttgtctttac cggtggtgga gcttctatta tcgacaaaca acttgtaaaa gataaaattc
     2761 cgcatacctt gtttgttgaa gacagtgaat tttctaatgt aaaaggcttt tacaagtttg
     2821 gtttaactca ataaaacagc gaaggagatc aacatgaata agggaaaatc agagcgttat
     2881 gtagttataa ttaataggga aaaggttagc gagttacctt tagcagagtt tcttgaatcc
     2941 gaatatgaaa ccctaggcgt gaaatactct cagacgatca aaaaagcttt gaacctatat
     3001 cggcgtgtaa agaacaatga agttacaatt attgaagcag gcaacgatag aaacgatagc
     3061 aaagtagcag aagtaaagaa taaaccaact gatcatatcc ctattcaaca agaacatcct
     3121 tcaacggtta catccgatca tttagcaaag aaagaaaagc caatgctaaa agggttcaat
     3181 gcaccaagta acaaagactt actttaaaaa gaaaaaaggc gaggttacaa tacctagcct
     3241 ttttattatg tacaaatata agcataaaca tacacgtata catgtatata aacatatata
     3301 taaacatgta tacgtatata accataaaca cgtatatgta atttagttaa gctataccta
     3361 accgcttatt ctaataaaaa aagtgatcag ttttaaactg atcactcacc aaaaacttca
     3421 gcatacaagt tatctatcat ttcaccaagt aaagctgaat ctgatcgaat tcctaacttt
     3481 tcgctgattt tagatagttt ctttctttgc gatggttgaa gcgtaaattg ataatttttc
     3541 tttttttccg agctatcact ttttagttta gctatcaaag aactagttgt ttctttaggt
     3601 ttttgtttag gtttttgttt aggtttttct actgcttcaa ctacttcagg aacttcttct
     3661 atttcagcat cggcaaattt tttaaatcta ttttctttca cttcaatcac tccctattct
     3721 tgtgccgtat caattatttt gttgtataac tcaaacgtgc tttcaaagaa atttttattc
     3781 ttacggtagg tgtccttatc cttagcgaac tcagcaatat tcttaccttc taaactagtt
     3841 gcattaagca attccttctc ttgtacatat ccaagataat tagaaagcgt tggtaccaat
     3901 tctaggaact cacgacttga attagtatta tgtttgatcc tgctaccgat cgtagcgtat
     3961 ttagtttcca tcagtgtttc accagtctta atatcaatca actgttttga cagtgcatca
     4021 atatctttac ctagcttcag caaagcttca aaaccatctt tagatggatc agaaatgcca
     4081 acgacaagat cagatacagc ccacgcattt tgagctagta gacctgcatc attatgagta
     4141 tcaatcagaa ttagatcgta cttctcttta agctgttcat agttaataac aatccatcta
     4201 aacagtaagg ttctgttgtt aggcatatca cgcatttgca tttcaacttg ttttaaatct
     4261 tcacctgcta ctaaaatatc catgctttca tttatagcta aaggttctgc tgatccatga
     4321 agatataaat gattgattgt attctcactt ttcgctttac tgaaagcatc aggaaatcgt
     4381 tttgataggt tttgcgactt atcttcatca atatacagca ccttcatacc taaattttcg
     4441 actgcatatc cacctagatt gtagatcgtt gttgttttac ctgttccacc cttttcaatg
     4501 tttccagtaa tgattgacag cttcttttcc atgattaaac ctcccttatt aaacttttgt
     4561 aactccatta tacactataa acacatttat gtatatgttt atatatataa atgtgtttat
     4621 aattatattt gcttttatat tctacaacat aaacatatat atgcatatgt ttatgtatat
     4681 gtttatatat atattgcatt cgtcctacga ttgtagtaca atgtaattga aaaggagttg
     4741 ataaactatg ccagtaataa gtattcgtgt atctaaagaa gaaaaagatt ttcttgaaaa
     4801 agttgctgaa ttcaacggag ataacatttc tgaatttatg cgtagtaatt ccctgcaaac
     4861 agcagaaacg ttagtagact ttgccaccta caagcaacta atgaaagagc atgaggaaag
     4921 cgatcaaagt atttcccacg aagatatgct aaaagagtta ggcttataag atgacttatc
     4981 aagtaagata tgaacgtgaa gcacaaaaaa cgttaaaaaa aatggataag tttcaagcaa
     5041 aggttatctt gaactggatc gagaaaaatc ttgtaggcac tgatgatcca agaaagcatg
     5101 gtaaaggtct aacagctaat aaatcaggct actggcgata tagggtaggt gcataccgta
     5161 taatagctga tattagcgag catgaagtga caattcttat attgtctatt ggtcacagaa
     5221 gtgaagttta taagtaacag acaagctgat aaggcttgtc tgttttttgt taacccaaca
     5281 aaatcactgt ttacgatccc actttaccta aaaaagaaca atatcccctt gacattatac
     5341 acactgatgt gtataatgta attgtaaaca agctaattaa cttttaggaa ggagtgacaa
     5401 tatgctaaca gccacattac agcaacctac tcacgttgct attgcagaaa gttatactat
     5461 taatgcaggg gtcgttaaac agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg
     5521 tcaagcgtat aacagacagt taaagaaagc tattctaaag cacaaaaatt ccttcatcgg
     5581 gaaattggaa aatgtggatg ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac
     5641 tattgacggt cgcttgattt ctctatttgc ttttggaatt aattgatagg gagtgaggac
     5701 tttggaaggt aagacttttg cagaaatttg gactgaattt attgaggtac caactcgcat
     5761 tgatccgata acttctgaaa aggtattgga tgcagacttc tacaatctca aagcaggtac
     5821 tagccttgaa tgcgtttgga tgtatttaga agagcatttc aaaggcgtaa cgctcggtca
     5881 cgtcttatac cacaatggtg ccgagtggga tgcaattgga aatatccatt tttcttaata
     5941 caataaagga gaacacaata tgaattatac cgatgaagaa caaatcactt tggaatacgt
     6001 tttcacaaat gaagaagggg aggaaattga aaaagtcttg atcatttcat caaaggactt
     6061 ggagaacgct ttggcttatg aaccatacga atcaaccgta actgtcaatg aattcttaga
     6121 tacctatact tctaatgata cttgggaaat tatgaatatt tgcaacgcta ataacattca
     6181 atttgctgaa aaaatcaata aatcatttta ttagttttgc aataaaacat cctctttttc
     6241 tattgattca atacacaata atgtgtataa tagattttgt aaagggaggg tagctatgcc
     6301 taagacagca agagaaattg taaaaattct caaatctaac ggcttcaaaa agactggaca
     6361 aactggcagt catgcaaaat tttataattt caagactaaa agaacggtcg ttgttccgat
     6421 ccacaatggt gatgtaccac taggaacaga agctaatatc tggaagcaag cagggcttta
     6481 aaagccttgc cttcctattt ggtattaggc atactatcct ctcttacact agaaaaggag
     6541 cgaggaaatg aaaacaaaaa atatttttta tccggcaatc ttcgagaaag aaggagaagc
     6601 ttataacatc attttccctg acttaccgga aatatctacc tttggaaata gtcttgaaga
     6661 agcatattta aacgctaagg atgctttagg attagcgcta tatagtgtac cagatgcaga
     6721 atttcctaaa ccatcagcag tggaagaatt aactttgaaa gccaaccaag tggtagtaat
     6781 catccaacta aacattaaac ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa
     6841 tgttagtgtt ccggaatggt tagttctttt agggaaggag aaaaacataa acttctctca
     6901 actacttcaa aaagcactag aagaagagtt gcttgaaaag taaaaaggag accgaaccta
     6961 tgaaagctac acacttaaac tatttctctc gaacgttaat caataatcta ttaaaagata
     7021 caactggcat tagcgttagt accaaacacc ttgccaatga tctaattatt tttgaggata
     7081 ataacttgcg aattgagatg tttattgaac aggcaacccc taactcaata cctgaattct
     7141 gctatacgca cactgaaaaa atgaaacatg caaaatcagt taaagatgtt gaagcattaa
     7201 gtgaatcact aacgaacgct ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc
     7261 aaaccgacta ccacaatgag ttgcttgctc aatctgaaat tttagattaa ttgaaacagg
     7321 agaagagaaa gatgaaacaa acacaaacag aaaccattaa cgatacgctt gccgatcaat
     7381 tagcaggtta ttataaatta tttgagatga gtaaagcaca gcgcttgatc cactttcttt
     7441 ggaaatacaa aattttgttt cttttttggg aagcaacact aacattctca attctttttt
     7501 ataagtcaga tttctataat caacacttag ttttagggag ttttttgctt atctacacca
     7561 ttttgatgct taatttatat acctacatca cacttgaaga aaacttagta attagcgatg
     7621 cagggatcga gagaaacatc aaaagggtat ctatcaagat cacgttaaac aagcctaccg
     7681 ataaaaagct ctcagaagcg ttctgagagc ttttgacatt tccttgtata atttatccta
     7741 acacattaca aagcacctaa ttcgagctta tatcaagcaa atttgctatt tattactcaa
     7801 tacatatcta aaaattactt gctggagaac tagatacatg gtaatcatga aaagaaaatc
     7861 aatttgatca tatgcagtat aatacatagc aatttaaaag ctctcagaag cgttctgaga
     7921 gcttttaata tattctttga taatttgtct ttcttaaatc catacgtcct aaaacaagct
     7981 gttattgctt aatatgatca tttgagagac atcaaatcaa cttttctctc aacaaatcat
     8041 tcaaagcttt cgacagatta atattctctt cagccgctaa aacatttaaa tagtgttcca
     8101 cagtcactga ttttcgatcg taattttgct tgtttcgagc tttagcaact ttcgtattag
     8161 cttttacgac aaataaatta acattctcag atcgcttgat tcttgcattt aactcttgaa
     8221 caggcgtagg cgatggaatt tctttttctt gcaactctaa aagcaccaag tatccttcca
     8281 atacttcttg cgccatttca aaagcatctt ctaaagtttc accatgcgta tataagtctg
     8341 gaaaatctgg gaatttaact gcatattcat ttcctttcca ttcgaatact gcacaaaaaa
     8401 ggtatttcat cgcattgcac ctctctaatt aaaaaataat ttttgctttt tagtttcaaa
     8461 aaaagaatcg cctaccaaaa aaattgatag gcgatcacaa gctaattaac ctctaaagga
     8521 gattaatacc ttgctcccaa ggcactacat atcttagcat acgtctgaag catcttcaaa
     8581 cgagtaagat atattttgca ttaacctaat tttttttctc tataaggttt ttcatcaaac
     8641 tcactagaaa tattaaactg gacagcaaaa ttgtaatttc tgccttttct aactttagca
     8701 aaatcaatct tcagcattga tttttcatta atttcctcaa ctgattccct taaaacacgt
     8761 ctatcaaaat tcgatattgt tttatactca ttttctgttt tcgtaatctc acgaatttta
     8821 actatttcaa tcaggggatt cttatatatt tcagctttct caaaatctcc tttatttttg
     8881 gcgataagat attttttata atacatcgcc aaatatttgt ataacacgat gctatatttt
     8941 ttttgaaaag atgctaagtc ttcaaaaaca aagaacggat acttcttttt gattgagaca
     9001 attaagggaa ttacatcttc tgaaaaactg aaagatacac tatgctcttc taaatcccaa
     9061 tgaatccgat caatagcaga tacagtagta tacgtcttat atttcttttc tagattcgat
     9121 atttcataaa ctgcttgtct ttgcaaagtg ttaactatgg tttttagttc aatttcaaga
     9181 ttttgatcaa catccatgta attaataatc gctttaacag gtaaatctac tacgcctgtt
     9241 ttctttagct ttgtgggtga gatataactg aacagcaatt gataaagttc atacgacttt
     9301 aaatccattt tcatgtcaat tgttgctaag aactcctcgt tgtactttgt cagttcatct
     9361 tttttcaaaa gcttttcctt ccttcacgca agtttttcag ttgttaatca taaaggttca
     9421 cataaggtaa ataactcgta attgattttt cgagatattt aacagtattt tttattgtgt
     9481 aatcctcctt atgagtagca acgtaagata gatgattgac aactgattca aatcctctat
     9541 ctttggataa acgatcataa agcggataaa ctttattctg aagccgactc ataaatttca
     9601 gatcgcataa gtcggcagaa ctgatcaaaa agtttttgat tagtagttta gtgtatttgc
     9661 tattcatagc tttcaagtac gattgattca ttttctctgt tttatcttct gcgcctttta
     9721 aataagcaag gtctctttgt tcttgcttat aaggtaccgg tactgacatg gtacgtgtaa
     9781 gcaagaactt tatatctgta taagtatgcc ccttcttgat aagctcatag tcaattttaa
     9841 aatcagtgta tttattcagt tgggcaatcg gttctttaat tacacgtctt tcaaagtctc
     9901 gaaaagcttt atattttttc tcaatactga atttctttcg taactctgca attgaaaaaa
     9961 cggggttttt taatctatca agtgattttg tactccatgt taagttatct ctatttgctt
    10021 catacagatt aaaatcttta gtgaataatt gaaaaaaact gatcgcatac ttgcttgtca
    10081 agttaactac ttgatcaaat gtatattgcg taaaatgatc ctctaagttc gttaataaag
    10141 gcataattga actactaaat ttaatagata ctgcatcaga aaaatcattg aattcagtaa
    10201 tttctagcgg tgagatgact ttgtattcag tcttaccgtt aggtttagtt ctttgaattt
    10261 taatgacaga ttgctgatgc aatttttcaa taacttgttt taatcttgaa tgttttgaag
    10321 aagatgaact agaagacatc aacgcaaata aagccttttt agataccttg acggtattat
    10381 taaaaggcgg atttttaggg tcaagatgtc ctatagcgta aagataaatt ttttgtggca
    10441 tagcatccat ttccacaatg ctatctacaa gttgattcga taaagtaaca gttaaatcaa
    10501 acttttttag attgctttcg gtagtttcaa atttcgggtt tacattcttt tctgttattt
    10561 tcatacgatt gcctcttctt tctaagcttg gtacgagctt aaaaacttgg aactggtacg
    10621 attccattga ataagtatag acctgttttc gatacgttac aataagaaat gtatcgtaaa
    10681 tttataacat aactcaatgt cagtaaaaca cattttattt tttccaatca aatttcatta
    10741 ataatacagc agaaatcgct attttagaac ctttacaaaa taaaaatcct tttcgaaaaa
    10801 atacgaaatt ataaaaatta ccaaaattaa aaaacaaata tttttctagt ttttagaaat
    10861 aaattcacat actttttctt tttttggata tattcggaat accacatcgc tttttgtcca
    10921 cgtttacatc gctttatgtc catgttcgca tcgctttttg tccaccctaa aatctattta
    10981 agattgatat aaagcctttt tggaggttgc taaagtttta aagattaaaa tatatattat
    11041 aaatataaat aaaaggggaa gctgaagctt cctccttttc ctttttttca attattcaaa
    11101 gttacacaag caaaataaac tcatagatag tgttagtttt tttaagattt tttattttta
    11161 agattttgaa ataagagagg agtaatcctc ctttattaaa tatctgttaa tatatctgtt
    11221 aaatatctgt taatatatct gttagggggg tcttaacctc ttgggagagt aagtattaaa
    11281 ggggtatgaa ggtaacactt ttggacagta aaggtaacaa aagtggacag gaagggtaac
    11341 acttttggac agtaaaggta acaaaagtgg acatttaagg tatcacatta ggataagacg
    11401 ttaccttcgg ttttcttgat ttggaaggcg aaaaaacaga aaattagatt agtaacaaat
    11461 ctttgttgac tgttaccttt agttgtgatt taatttaggt aacaagcaga aaaaattgtt
    11521 tcctttaatt agtaccagat taacatgatt aaaggagcgc aaaaagaaga ttggagagta
    11581 atcaatgtct caagaagtta ccgaaaaggt tattgatagt gatgtagatt tagtatcaga
    11641 acaaacaaat gcaattgtgc gttataacaa tggtctgaac acaacagctt tgcgaggatt
    11701 taagcctgtt gaaatggatg tgttttgggc tattttatca aaaatgaaac gtcaaggaac
    11761 agcagaagta acttttgatt tcaacctctt tcgttatttg gtcaaatacg acagaagaga
    11821 aaaggacagt ttttattctg tgttaaaagc tttgagtgac aaactaggta ctttaaccta
    11881 ccgttttgaa gattcagacg tctatgaaca gctgtggctt tttcaaaaat tccggataga
    11941 taaacagaag gaaacagtta caattaaggc aaatgaggaa tttgaattta tcttaaactc
    12001 aattggcaaa aactttactc gttttgaact tgataatttt acaaaactaa gaagtagcta
    12061 cacaaaagaa ttgtatcgtc atctcatgca atacagaaat aaagagtggc gaaatggtta
    12121 ttgggtagtc aaagttgaag attttagaag agcattaagt ataccttcaa gttatagaat
    12181 gacaaatatt gacaaaagaa tactagagca ggcgaaagaa gaatttttag ctcctgatga
    12241 aaacggaata caggtatttg aaacttttga ttatgaaaaa atctatgcga agaaaggaag
    12301 aagagttgat cggctcagat tcacttttag tgagccagaa tcaaatttgc caaccatttc
    12361 aatgcataat tggttagaag aagattgaga agaagcaggt tgtaatttta atacaacctg
    12421 cttctttaaa aaagaatggg taacactttt ggacaataaa tgcaagaagt tttttttaag
    12481 caagcttcag tctaaataat atttaaaaga atggctaatc tatacaaatt ttaacaacta
    12541 agggtaacac ttttggacaa gtcaagaaaa aaaacaattt ttttaatttg ttgatcgact
    12601 aaaggtaaca cttttggaca acacagaata aaaagtccgg tgatatcagt tttggtagaa
    12661 aacatcttaa tatctttgtg agattgaagg taacattttt ggacaagtca actatttgtt
    12721 aaaagttaat atagagtaat tagataagtt ttttcttttc gtctgataaa tggataatat
    12781 tagttatttt attgttaatt agtctttcgt gatgactgaa atagctaaaa taaatatctt
    12841 gttgaataat tgctgataat ttaagggata tctaaatttg ctgattgatt tatttatgtt
    12901 attatgatat tataataaca taaatgtgtt acgcccgaac ctcattttaa aaaaatgaag
    12961 gggtgagaca atgaatgcta ttaattggca tgaatttgtt aactatatgg agagcttact
    13021 agatgatcta aaaagtttta gtattgctga tgcagtaaag catgggttac cagttagtac
    13081 aacgatgaaa ttacgaggat tgaaaattcc agatgaaatt attaagctga gaactaaaac
    13141 aattattgaa accgcaaaag tcgttgacaa tatgaaccat gaactaaaag gcaagaaaac
    13201 aacttacgag aaagattttg aacgtttttt aaaatctgtt attggaaaat atctaggtaa
    13261 aaaatctttc agtgaattga gagatgaaga tattgtacct actccaatgg ttagcaaaat
    13321 ccgaaaaaac aaagccgagg ggaattacta tcttggtaca atagcaaaaa tcattgatta
    13381 tttggaaaat aaagagcaaa agaaaggaac gaagaaagga agataatttt tgtgttaaat
    13441 attaatgggg aagaatatac cgaaatgatt caaaatcccg aacctagaat taccgaaaaa
    13501 acgtctgatt gtttgctctt ttatgaaagg gggcagacaa actaatggaa aaaaacgtca
    13561 attcaacatc taccaaagat caatacagta ttattggagg gttgcttggt gataaggata
    13621 agagaacacg tcacggatta cgcattatgc ttgcaacttt tctaggagtg ctttttttat
    13681 taacaggctt gggtagctac atttatcaat taggaaacta taattattat ctcgtgaaca
    13741 atacaacacc tataggctct tcattggagt ttgctcgatc aggagcagag atcactattt
    13801 cagatgtgtg gacagatcaa aacagagatg taacagtagt aaaactcggt tactcgcaaa
    13861 acgctagaaa gttgttatct acgcaaggaa aaaactacaa cttatatatg gcaacgaaag
    13921 aaaaagatcg tcctaaaaag cttgaaatga gctatgggat gttaagtact gatggggatg
    13981 ctttcttatt cctaaaaggt aaattagatg aaagagctta tcaagttttt attgcaaacc
    14041 aagtatcact aatttctggt gatgatacag ctgacgatga taccaaagtc aatgtgaatg
    14101 aagcagattc agttacaaaa gcactttctc aatattctat gaatgatgtt aataatgaag
    14161 gagtaatttc ttttgggaaa aaagcaaaac ctgttaatac tcctgataat atcaatttca
    14221 gaataaatcc atattcagat tcaactaatg tctatcaagg aagctttctt accaaagatg
    14281 gcgatattga ttattcaaaa gttgtagcag ttacaagtaa gaataatgtt gtaaataagc
    14341 tagaagatca gttaaatgct tcaaatacta acatcaagaa aattgaagcc acaaaagctg
    14401 agtatctgga acgtttacaa caaaatcctg acgataaaga ttcgcaagaa agtattcgtc
    14461 aactagacag tcgaatggag acagaaatta aagcaatcaa tgatacacaa aaattgattg
    14521 atcaatacaa aaatgctgac tttaacaaag attcttttgg agacatgaag acgacattta
    14581 agtacctaac tctgactgac tagaagtaaa ttattcttat tttagaggaa gtgaacacaa
    14641 acagatggaa aatagtaccg agttttttaa gaatattcaa actgaattta atcatgctct
    14701 cacaccaatg aaaccaccga aacataagtt agtgggacgt gatgacgaac tccatgagtt
    14761 gtcactgatc ttgaatagac cggaaacacc gcaagctatc ttgttaggtg aagccggaac
    14821 aggtaaaaca gcacttgcaa gtgaatgggc tgaaagacag cgacaagatg gaaagaaagt
    14881 acaagtatac aaattatcaa tcggacaatt gcgtgttggg ggagatgatg tcctccaaac
    14941 acgtttagaa acattaattc caagattgaa agaatatgag cgaattttaa aatctgagga
    15001 caaagaaaat gaatttgtcc tcttcattga cgagtttcat aaagttgtta gctcttttgg
    15061 tagtggcaat aaaattgggg gtgattcgct aaaagaaccg ttagcaaatg aatcacttca
    15121 attgattacc gctactacaa gaaaagaata tgaaaactat atcaaaagag atgatgcctt
    15181 agggagacgg ttacaaccgt tagaattgaa tgaactaaaa agcgatgttg tcgtcgatat
    15241 tttgagacaa tggctaaata catcctctgt taaagatgat cctcaggctt cttttaaatt
    15301 atcgcaaagg attcctgatg aattacttca agaaatcgta caagcaaaca agctttatcg
    15361 tgaggatttg tccgaaccgg ctaaatcaat tgccgtaatg gaggatttga ttgcaaaaga
    15421 tcgagtagac ggaaatgctt ataatcgaga aagtctattg caaatatttg gtaagtataa
    15481 tattcagcta gattctgatt ttgatatgga aaaacttcaa gaggaattaa agtccataaa
    15541 agggcaaccg ttagcagttt atactataac aatgttgttt aaacgtatga agcttggatt
    15601 tgatcgtttt aaaggtcgaa agcaaccaaa agcaactgct ctgtttacag gatcaacagg
    15661 ggtcggtaaa acagcaatgg ctaaagccgt ggcaagagga ctttatggcg atgaaaataa
    15721 agttctctta gttaatatgc cggactacaa aaccaaagat gatgcaatgc gttttagacg
    15781 actcataggt actcatgtaa ggcatgatcc ttctgcgatt attgtgtgtg atgaatatga
    15841 gaaagctggt gtagaaacac tcgatgcctt tttatcaatt ttagatgaag gtaggataag
    15901 ttttactgaa agaggatggg atgatcgaga agttcacgac accgtttcat tgtcaaatac
    15961 aattttattt gcgacaacaa atgcaggtgc ggagatgttt gattcgttaa acagatatag
    16021 ctcactatct cgtgttacaa acaaggaaaa ttctaatgaa tatagcactg aactaaaaag
    16081 agaataccga aaagtcgcat cagggatcaa acaagccttg attggtgaag gtctaaagcc
    16141 tgaagttatt aaccgatttg gacgaatagt tccattttgg gctttatccg aacaaactct
    16201 tcttgaaatt gcttataatg aaatgttgaa aagtgctgaa ttagtacgaa aacatttaaa
    16261 tatagattta cgtcttccac ctaggaaaaa aagagtatat ggtcaaaatt tttatgcaga
    16321 cgatctctcc atgcacttag tttttgacat ggcttcacaa gaggattcta aatcaggggg
    16381 tgcaaggaat atcttgcatt tagttgaatc tgagtttgaa gaagcgatta ctgaagcaat
    16441 aattacttat ccaaacgcaa aaatattcga tgttaagttg agtgaagccg gagcgtttga
    16501 aggaaaaggt gattctaatg ctgaaattat tgcaaagcct tccgttgctg aaagtaaaga
    16561 aaacaagatt gtattttcga ttggaggggc gtagctatga aattcaagaa atatttacta
    16621 caaaacctta gaataaaacg atgggtcgta agaggtctag ttattggagg agtattagca
    16681 gttactttag gcgctccttt tggaatgaat gatccaactc ctgacaatca agatagcaaa
    16741 caagaaacag ttataaataa gaacgttgca tatgcggaaa gtaaaccggc agaacaagga
    16801 atgtggacac tatatgctcg tatcctaatg ggagataaag aagttaatga tagtgccgct
    16861 aaaactggac aaatgaattt gttaggacaa ggtggtatca gtgccgattt cccttattct
    16921 gacatttcta aacaaggtaa ttcgattgca gggggtaatg caggtactca actagcatct
    16981 actttagcaa cttatagtta ctacggatat attgaaacag tctcaggaaa tgctattgcc
    17041 agtcaagcat catccattct aggaagtatt ggtaggacga ttggtagttt tatcggttca
    17101 attagtttaa tcctttctaa tattgctgaa tccataaatg aactccttgg aaaatttttc
    17161 atgagtatga atatattttc tcttatgggg tggggagaag gtggatcgga tgtatctaat
    17221 ccggtatcta aagcattaac tagcttgctt aacaatttgg gattaaataa tgaattattt
    17281 aaagcgatag caagcttatc tatgctcttt ttcacagcaa tactaggtta tcaaggaatg
    17341 aaattattgt cgaaagatgg tgttaactcg aagggattat caccagtact tgaaaaatgg
    17401 attgttcgta tttttgtatt gtttatattc ttaccagtgg caggatcgct attgggagtt
    17461 tatatcaacg gcattcagtc agcaaaaaaa gatggagata taccaacaaa tgttgtttct
    17521 gactacatca ttaacacaag actttgggct tctactcaaa atcttgctcc atcatctgat
    17581 actggtggtg atgtacctga cggaaagcct acatccgaaa tcaagttcgt tgatacagat
    17641 tatgaaccat ctaaaaaatc tgcaatgata agagcgatca atactagcag ttatgccaga
    17701 caaaataatg atctagattc taattcagtc ggtatggctc tattaaaaga atggtcatcg
    17761 aatgatactt tcaatattaa tacttatgcc ggtgatgtta atgtcgccga aaatgatgct
    17821 tatatgggaa gagcgcctgc tgtttggaca ggatataatt taggtgatgg aaaagaagga
    17881 agtgcaatta agaaaccaac caatttaaat ggatatattt ggtcagcaaa tcaaaatgtg
    17941 acagacgaca atcgcaagcc agatggaaaa ggaacagcgc ataactttga tgctactcaa
    18001 ccttggggcg ttgctaacaa tgcaactttt agcacacagt cggtagcttt actgctccaa
    18061 tcggcacttg atacatcagg agctaagttt tatgcgtata atatcgctcc ttcaggaatt
    18121 caaggcagtg ctaagaatat gtcaacagta aaaacagaat ggcgtacagc atccttagtt
    18181 ggtcatggag cgatgggtaa gtttggctcg tggctagcga tgaacgctga atcaatcgtt
    18241 ttagcagttc tttacttagc atgtgcttgg gcgatgttca atgtgaatat cgtgaaagca
    18301 tttaaacgtg aatttatgcg gatttgggaa gtagttctaa caggatcact tgcatcagga
    18361 gcaggtgtga tggcaatttc attaggtgtt gttacaacat ctctgatcgg actacaatta
    18421 cctatctttc tgattacttt tattcgtgca tttggtgata ctgctgtggg attcattcct
    18481 aatacattag ttggcgatgg cgtagcaaat attgttcgat ctgctatgat tttaggtgta
    18541 ggatatcttt tagtctttaa aaaaggcgaa aaagtaaata atttgattaa tatgcttatt
    18601 ggcttaccac ttgaagtagc gtttgggttt gatgaacgtg ctaagttatt atttggtaat
    18661 agaaatgatg ctgtgcgagt tgtggacggc gtttctaaag caatcaagaa aagccaagga
    18721 gctaaaaatc cctattatga tggtggcaac agctcagtaa aagatatggt tgcaggaggt
    18781 cttggagcag ttggagcgca aaaaatggct gaacgtttga acaatatggg agatagcaat
    18841 gctgatgcta agtccattgc tgataaagta attcctgaaa caccagaagg aatgtcattc
    18901 aacggtggcg gagttggttc aacaatcaat aatacctctt tcaaagattt agttcgtcaa
    18961 gatcaactac gtaataactc ccaacaatat attgatcatt taggacgtat ggttgatgaa
    19021 aacggaaact ttgtcgatga aaatggtgaa atattaaaag atagtaaata cggtaaagat
    19081 gctaaaggca actttattga cgaaaaaggg cgtttggtcg atgaagatgg aaaccttata
    19141 gatactaatg gtaaaccatt ggaagatagt gaagatggta atattggtat ggatagcgaa
    19201 ggacgtttaa ttgatgaaaa aggtcgctta gtcgatgaag atggaaatct tgttgacgaa
    19261 aatggacgca ggttgctaaa tggtgatttt ggtcaggata gtaacggacg tttagttgac
    19321 gaaaaaggtc gtttagttga tgaagatgga aatcttgttg atgagtttgg taataaactt
    19381 ggtaaacaca gcgatggata taatgtgaat ggcgatttag tcgatgagga tgggaatatc
    19441 attgagacga gagatgcgct tgtggcagat cgaaatagag aactcgaaca acaacggcat
    19501 acgccaattg ctgttgcaat tcctgagaat gaagatgacg agattacttt agatcaaact
    19561 catcaagaac tgaggaatga aatgagtggt aaggacgtgt cagacagagt gtatcgggaa
    19621 ttaggagatg gagcatctgg tgaaagtcag tcaagagagc ttaacagcaa ctttgataat
    19681 agtgctattc ggaatgacac agcacttaaa gacagagaat cgctaacaaa agaaacggat
    19741 aaagaacaat cagatgtgaa gaaaaagttc attcctactc ctaatgttaa tcaaacagat
    19801 aaatatgaaa caaatattgg atcaggaatg tcgaaagaca atcaatcaat tagagaacct
    19861 gaactgacaa atgaaaataa tagaacaaat aattctaaat ctacttcaga taattcaggg
    19921 aaatctaatc aatcaaccaa gaaaccatca acagctaaat cagccgttga atatctatca
    19981 aaaactggta aaaatgttta cacctcagca gataaagctt tagcaaaagc tccaaaaggt
    20041 agtctagcag ggataaccta tgaagctaaa aacgaagcta ttaaaatagg taaaggaacg
    20101 gctaaggttg ccaaggctac tgctacagga gcatccaaaa ctggtaaagt agtgaagaaa
    20161 tctactatgt cagtcaagga ctatattgta aaatacggta atcagaaatc aactgaaaat
    20221 aagtctgaag acgagtaaaa agaaggagca ttgatttgct ccttcttttt actttgattt
    20281 ctaactgaag atgtcaagga aaatttctat ttatgcaaaa aaattatttt aaattgtcta
    20341 taaatgaatt aaatatttac tgttcaaagt tgtataatta aaaagggctt atgctggtaa
    20401 agaacagaat tattatctaa acaaaaactt gataaaccag catttttagc caataaaaaa
    20461 agtgaaataa tttaatggaa aatctctttt aatcatcgaa aatttatgtt attatgataa
    20521 cataaagatg taaataccga gacgtctttt tcttagttag atcattgaca agtgaacaaa
    20581 taagatttgt cgatgatata attaacttat taaaaatact aaaaaggagg tttttatatg
    20641 ttcgattcta ttaaaaattt ggttagcgga atgacgcctg atatgaaaac tttagtacaa
    20701 gttgttttgt atttcttggc attcgttttt tcgttgaaac caggattcac tgcaatgaaa
    20761 ttttttggtg atgcaaaatg gggagatgga ttaaaatatg ttggggcgat tattgcaatc
    20821 tttattattc ctgtaattac aaccgttatg ctttacacaa tgggtactaa aacaggtaat
    20881 gatttgaacc aaaaagctaa tatcatatca gcacttattc cagttgttgg tatctatcta
    20941 gtttcaaaat ttccaaaaaa tgaacagtta ggttaaaagt gaattgaaca aatttggata
    21001 atacgccgat ttgttcaatt tactttttac caaaaaacta cgctccgagc gtagtttaga
    21061 agtgagggaa gaaagtaaat gtaagatggt ttttgataga gaaaaaatgt tagctcacgc
    21121 caacgaagta ttaatgagcg gtctgaaagg taccgaactg gcaaagataa tgaatatgaa
    21181 tgttaaccag ttctatgatt atcgaaatgg gagtaaaaag attgaaaaag ccagacttga
    21241 aacgcttatt agattcgaaa aagcatatgt atatatgcta gacaaacaga aaagaactat
    21301 tgaccgaaaa aaaggagttt tacaataatg gcaaaattca aagatattac cgaatctatt
    21361 aattatgtgg cagaaacagt acctgtagtt gcactctctt tgaagccaca attactcgaa
    21421 gtgtaccaaa aagaagaaaa agaagcgaag gcaaaaaaag ctaaagcaaa ggtatggaaa
    21481 gaattcgatg cgttgctgat caaagattta ccaaagaaaa attatatggt gattgaagat
    21541 gtacaaaagc tcaacagtga ggaagttata gatgaggatg atgctattct cgattccgaa
    21601 aagtatgtcg ttctattgaa gtcaagtttg aattatgtag ctgatctacc agaaaaattt
    21661 aaagagaagc tattagcagg tgtccgtttt atggatagta gtggtcaatt attacttatt
    21721 ccggatgatc ctaatatgtt tgaatcagaa gttgaagaag ctacagactt ctatagcgca
    21781 gtggagcaat tagaggtagc tacaaatgga ttagaacgac ttgctgagac cttcaactct
    21841 tctgaagggg ttcaatttga agctgtagca gaagacgtac cagatgaaaa agaagctctt
    21901 tttgtgtatg atgacaaacc aaccgaagca gaaaaccaag aagtaagcat agaagaaaca
    21961 caaatacaaa cagaaaacta ccaacaacca aaaattgatg aaaaacccga attccagagt
    22021 gacaaagaaa attctgttta tgaatttgaa aaaatcgtat ctgaaagtga tataccgcaa
    22081 aacttagatg ctgatttttt agaaaaaaat tttgaacaga tcattgctga caaatttcaa
    22141 cctgtagttt tagatgagat taatgaccct gtcgatttaa acgaggagat gctatccata
    22201 cctgagttga aaccattgct gtttttagct aaagagagta tgaacgctcg tatctctatt
    22261 gctaataata aaatcaacag tgaattaaat aagacgaagg agcaattgat cctcaaagga
    22321 aagcaaatgt tagccgatga gcttttgcgg atagaggatg caacctcatt aaatggtcaa
    22381 aatcagttta caaatacact atcggttgct aaagagagct acgaagcaga agcatctaaa
    22441 attgatgatt tagtgatgga aaaatccaat atcattcgta aagattatga tatggcgaaa
    22501 caggcttatt tggatgctga actaaagaaa ctagagcaac ggtatgatgc tgaacattta
    22561 ccagaagtta aacaacaaga agatgaatat agaaaagcaa ctgaagaaag tcttgatcaa
    22621 cagtaccaag aagcaattga atctattaaa aacgatgcat tgaaacaaag ccaagaagca
    22681 aaagaagttg tggtggaacg aattatcaat tcattgaaac cagaaattga aaaatcatta
    22741 acagacgtgc aagaaaaaac tttagagatt gtcgaaaaaa caaagtcaga aaatgaagct
    22801 gagttccatg atacaaaaga gaaaacaaca gatgtggttg tcaaatattt tgcgaacaaa
    22861 gaagaagttg aaaagcaggt agctaacaac acgaaagaat tagaaacaaa acttgtagag
    22921 caacaaaaaa ctatcgaaga atataaagat cgaatgaaag aggtcaaaga tttagcagag
    22981 ggttataaat taaatagcga aaagttggca gaacaaagtg aagctttaag agaagactta
    23041 tctcgttccc gaagtgatct agctcaaatg gcagaaaaaa cgaaatcatt agcagttgct
    23101 acagaaaatc aatcaagttc aaaacgtgat tttacaagta aaaagatggc tactatcatt
    23161 gctgtcgctg caatggcttg cggaacgatc ttgggatcgt cctactttat tagtcataat
    23221 aatcaacaag cacaggctca aactcaaaca tctatttcga gagtggaaaa ttcgattggg
    23281 acaaatacga actctgcttc aacaaatagt agctatgaag caacaccaac tacagagttc
    23341 aaagttggga aagatgttgt catcagtatt gatgggaaaa caactaaggc tgaagtgatt
    23401 tctattgatg gagaaaaagc agttgtgaaa actccagacg gaaatcagta caaaattcaa
    23461 gctcacttat ctgataataa ctaaggaggg ctattatgcg agaagaaaaa atagtttatc
    23521 cagtaattca aaacaatcaa gaaaacatga cctcttctca gcacttacag gaagcacagc
    23581 tcgtgtatca ggtaaatgaa ctaccacaaa cgaacgagac aatgagcaag tcaatatctt
    23641 taattggctt gcttattgtt gcaatagtga taactgtagg tgtttaccgt aagttgaacg
    23701 tgaaaggaag tatttctgat ggcagataac tcaaactatg gatttaaaca taacttacat
    23761 caaaaacctt ctgaaacaat tgtcagagtt gaatggcttg aaaagcatgt caatagcacc
    23821 tattctgtta cagcaaaaag attaattgta tcagctatag ttgttgcagt cgttgttctt
    23881 ggatcaattg tttacttagt ttctgatacg attaaactga aatttatttt tgttccaacg
    23941 ttattaatga taattggatt tcttattcta attagccagt atatgtttat tgatccatct
    24001 agtggttcat tgatttctgg ttctattaga ctgttcaaat cagtagttaa ttcagcaaga
    24061 gtatctatgg gtaaaaagaa accatttcga ggcacagaga ttttaaaagt gaaacaagat
    24121 ggaacaatcg tatatcgaga tgggagtgta gcattaatgt tcaatctaga tggggctaca
    24181 tcactaaccg cttttccaga agaagttatg gctcaggaat cgaaatcaat acaatatcaa
    24241 aataccagag atcgttcctt agctgaaatc aaaatcactt catctcaacc tcagaatacg
    24301 gaacgacaaa ctcgaaatat gaaaatacaa aaacgaaatg caaggagtaa caaagcagtc
    24361 tttgacataa tagatcagca agaacgattt gtatcagaat atattgatgg tgtgaaaaca
    24421 acagtaatcc aatatcttat cttgaaatct caatctaaga tggtattgga tgaaggtgtg
    24481 gaacgtttag aaaactatac gagtagagga ctttattata gtgtcactca gttgtcagct
    24541 aaacaaacaa aagaaatatt atctgatatt tatgcactga aatagaggtg agagattaaa
    24601 tggaaattat gggtagatca caactattaa atttcaaaaa aaagagaaaa actaaaaaag
    24661 aacaaaaaac tagaaattca aaggtagtga ataatacctt tgttagtgat ttaacatctt
    24721 tagttggtta tcaatttaca ccatcttaca ttaggacagg taagcggtgg ggaacaattg
    24781 tgaaattagt gaatacttat ggaatgaata gaaattatgg atttggttgg atggttaaag
    24841 ctattcctga agtgagacta gaaggggtaa aagcctatct aattgaagca gataagccag
    24901 tgtcacaaaa agatcaacaa gaaataatgc acgagaatgt tgggggatca acgaatagtt
    24961 atgaaaataa tttagaagga aaaaattcag atgctgaaat tagcaaaact gttatgcgga
    25021 ttatggattt gaatacagca tctttactag acagcatggc aaataaaatt attgattgga
    25081 agctagaaat cctattagta tccgattctc ctaataaaat cagcgaacag ctaattaaac
    25141 tccaacggtt gtatgatgaa aaaatgtatg gattaaagct tatgtcatat ggaggagatc
    25201 aacgtgatct gtttgataga cttttaagtg aaccaatcgg gaatgtctat gagtatacgt
    25261 ctatgagttc ggattttgca ggaaatgatc atgcaattcg taaaggttta gatgatgcga
    25321 atggatgggc tatcggcact atgtctacta gctacgcaag tggtgtagct tttatggcac
    25381 tggatgattc ttttacaagt aaagttttag tggcatcaca caaaaacgct acaattaaag
    25441 gctatgatga aaaaatctca gcatcatcac tttgggggca attgattgct aataatgcaa
    25501 tggcaaatca taaacgaact tttcatattg tcttaaataa atttgattac tttggtgatc
    25561 caactggtag aacatttgtt gccaaacctt cgttaaacag tgtaatagaa aaggttaatt
    25621 tggctgaaga aggtcttaac tctttggagc tttttggtaa tcaaaaggac attggtgatt
    25681 tatatagacg gaatctcgat aaactaactc agatttttta tttgttagca ggcagaacat
    25741 tagattctgc aactagagtt ttattgaaaa aagaattagg taacttttat ataagtcaga
    25801 aactttggga tattaattat gaagaacgtt caaaactttt ggtgaacagc agtgatacaa
    25861 tgccagttct tggggatttt attagctatt tgactaatgc tgaaacaaaa gccaaaaaat
    25921 ctggtactga aaaagaagtc gatcgtatta ttgtattaaa agacacattg agaacagcat
    25981 tagacagctt ctctaggatg tttaatcaaa gaacgacact aaaagaaatc ggtaaaagtg
    26041 acaaattaca gttttactat aacttaggtg atctaagagc agaaatcaat gttatggaag
    26101 ctcaattttt aaataccttt gatttcatca catcacaagc aaaaaaagat gatattgtta
    26161 tgattcacgg cttcgataag ttatctattg aaaccgttgg gctaattaaa aaaagattaa
    26221 caatgcttga agaagatgga attcgtcttg cctttctatt tgatacaatc ggttcaggag
    26281 aagacaatga atacaaagaa gatattgaaa cagctgatat attcaataca gatggaaaaa
    26341 tttaccaaag ttttgaaagt gattttaatt acactatcat tggaaagatg accagtggag
    26401 agttaatgaa gtatgaaaaa aaagttgctc ctgctagaaa aggagaagat gatcaaaaac
    26461 tttctgttga tttgagacaa tatttgacaa caggaagaag taataaattt cagatcagga
    26521 gaccagtgga tctgactaat aattttgtta ttggagagtt tattctttaa gttttatctt
    26581 tgattttgag tgtataagga aaggataaga aaaaacatgt atatttgaga ctaaaaacag
    26641 tcttagagaa catggtacgt tttgtgaaaa gagaactata gaaatttcat aaagcgaaac
    26701 cttactgagt gaaataatta tagcggaggt taaataatga aaccaaacat aaaaatttta
    26761 gatcgaatat ttctaggtag agacacagaa gtaatcctaa ttcaacatga agaaggattt
    26821 gaagttagta taggtattca gaaacttcaa aaacctcatt attgtaatca gttgtataaa
    26881 aactttactg atgaagaaaa agcgagagtt ttttttaatg aagtcaaagg tatgagagaa
    26941 cagtatgagg tggttgaagc atgaataagt taccagaaaa gaaccgtgta tttagattga
    27001 aaggtgatct cagccaattt aaaatagatg agaatggaca attttttgtt tggatgcgac
    27061 atgagcattg ttggtgtaat ccttttgagt taaaaaaaga aaatgaaatc agaactaaaa
    27121 ttgcaaacgg tgagtgggat tacctcttca ggtgggaaga tgaatctttt atagcaaatg
    27181 atatggatca tgcatttgtc tcatttctgg ataatacatg gggtacttgg tacgattgtt
    27241 atctagaaaa attgggcttt caaaatattg atattgatgc tattgaatgt cttaaagaag
    27301 tcgatcaaaa gtatgaagaa gaaccctatt atctcttgca acaaaaatta gccaataatt
    27361 cagtatttca ggtagaaatt tgggaaaatg aagatggaac ttatgagggg ctagcgcaag
    27421 aaataccctt ctcagatagg aagagttcga tttgaaaaga gtatatcagc aatttttatg
    27481 aagtgaaagc aagcttacaa tatagtaatg gaaaattgtg gttagctgga aggtatattc
    27541 tagctaacca aatcttacgt tttctgggag agtggcgttt taaaacaatt tcctaggaaa
    27601 aatagttaac ataaaaggaa tgaattgtta tgaaacaata tttaaatgaa tggaaagcga
    27661 tagaggggtc attagtaacg caaagaataa aggaattacc tgattgctta gagaaggatc
    27721 atttgtttca aatacgagaa atgttgaaaa aagaacagtt tgatccagac caatttttag
    27781 tagtcgagta tccaactatt ggagtgtact gttgtaacca tatcaatgat ggaaaatatt
    27841 ttattattca ggagtatgga ggacagttaa ctcctttcta tactacatgg gaaatgagtg
    27901 aagaaggaat caataatttt ccatgtgaaa gcatcgaaga ttcaatttca cacaccgaat
    27961 gttaattgat tataactata aaaaaggatt gaacattatg ttcaatcctt ttttcaatga
    28021 aatgttttcc gccatttcat tgtttggtgc gatttctatt ttattatatc aaaataaaga
    28081 gagattctaa agaaagaaga gggaaatata taaatcaagc gacagtatat ttattattaa
    28141 atgctattgt gagaatataa ctaattagtt taatttgaaa gtaaatatac gctataaaag
    28201 tgcatcttgt gttataagaa attattttat tttgtgaaca taatttaaca attctttctc
    28261 ggaattgatt tcttttctta aatagtcttc agacttatta ataatattat ctattatgtc
    28321 tagatacata tcacttttcg attcagctaa ttcatacagg ttttttaatt ctttatcctt
    28381 taattgaaaa ccctgatcca tagtcactgc atttggatat agaagcccac tataatgttt
    28441 acgtaaattc acttggaatt tctgattata aaagtctaac agattagaaa taaaaggcgg
    28501 aaaattactg tttttgtaaa attgattatt tccatttagg ttgtatagta atttgttaaa
    28561 ttgctcctga tttaatttat tgtaaatcca tggtaatact tccatatcct tatctgctaa
    28621 cctgaatgaa ctagcatcgg taagatgttc gatcataaaa tctatgaaca tctctttttc
    28681 accaaaatat aaatattgat tatatattgt ttctaatgtt gaagatggta gcgaagaaac
    28741 tctcattttt tgctgaagta taggatcaag tagactattt aagtgatttt gtaattcttc
    28801 attgttgctt cctagaactg caagtgaacg taaacggtag taatattcta tttctgttag
    28861 tacttgttgt ttcggtttaa aatcatttat tgatatttgt aaattttgaa ttgtctcgtc
    28921 tgaaaaatca tgttgtaatt ctggatgggt agctataaaa gtaagtaaaa agaccccttt
    28981 tgtatatgtg atctgttgat tttcaccaag tgatttatta atttcctctc ttgattcagc
    29041 tgtgaaccta tactttgaaa tttcttcttt tagaattttt aaatagaatt ctttattatc
    29101 aaaaattagg gcattaagta gtctgaaagt actttcccta tctctatctg cttcctcatt
    29161 tccataaggt aattgaaaaa caaacttaaa ggctgtctta cataggtttt cttttgcttt
    29221 ttcgcttagt ttatttagat agctattttt tagtgcagag tgaaattctt caggggtaac
    29281 agacatagca tgtatgcgac ttgcatattg tataatttcg atcgttactt tttttcctaa
    29341 tattggattt tttaggaata taatttcata ggacttccgt ataacatcct gacaagtttc
    29401 ttttgttata tctcttaatt cccaagtatt accgttgtaa gtgactacag gatgagctga
    29461 ataatttctt tgatccttaa tatattttaa atcctcatat tcatagctat caattagctc
    29521 agttgaacta taagcttttt ctagtagttc catttcccat tttggagaat ttggatcact
    29581 ttttcttaat ctagctatat cttctaaaat tttcttagaa gtaatatcct cgtaaatatt
    29641 tgctaaatca gataatttat caattaaatc taaaacaagc gcactgtaat tgacagctat
    29701 tgcagaacga taatttccct gataaaaact acttcgaact tcttctaata atggaataat
    29761 tctaggattt ttaacttttt ttatttcatc atctaatata aacatataaa tctccttttt
    29821 ttgtttgtat gacaagttta ccataaaaaa atattaaaaa gcgagacaaa tttatgttat
    29881 tatgttatca tgatagcata aatatatgga gcgagatgat ggaaaaatgg atttgaaaca
    29941 atcgttttac atctggttat atcctgataa tcctatgtca ataatggctg atatttttgt
    30001 agttagcatc ttattgtatt tattttattt aattttcttt cacaatttaa tcgggtacat
    30061 gattcttgat tggaaaggaa tgttcgatat agagagaaaa ccatttgcta acaatcaaac
    30121 ctataccgtc ttcgaaagtg gagattggga agaaaatgaa gttgatgtga tccatgaaat
    30181 tggattagct ttatcaggaa tacaagatgc tgatgttttg gtatcaataa caacggatac
    30241 tggatacaac gcttatgagg tattttatct tatgtatttt atatggcgtg atggtcaaga
    30301 aattatcaat ggtcgtgctg aaaaaacaat catttctaat actgaaattt ctttctgtat
    30361 gggtgaaggg gaaatgattg tatataaaga tgggtcagca aacattaaaa cagcctttgg
    30421 tgatattgat acaagtatta aggaacttag ggaatatgtg cagaacccat ataattttca
    30481 atatttagca ggaagaataa atttagttta ggagtaaaaa tatgaaaaaa attattaatc
    30541 aaccaacaaa tcacttgata attgaaggtg ttattttcgt aacaatgtat tttactgtag
    30601 gaaatggcat ctcaaataca atcattcaaa aaaatccaca atacctatta tttagtcttt
    30661 atgcttggat tgtgcaaatg gtttgggttt ccatattgat gctgttgttt tattggatag
    30721 tacgggttat tcttttagtg ttaaacaaac gaaaaaagga taggtgaata aaatgaaatc
    30781 aactaaatgg aagattgtat tgttatcaac agcagcatta ataactctta cagcatgtgg
    30841 tacaactaat agtgctgata ataacaaaga cacaaagtct agtagtacgg tggttactaa
    30901 aaacagctct acatcggcat caacaacaat ttcttcaatg actgaaaata atgctgaaaa
    30961 gtatcaaaaa tacgaagaca ttaataaagg tcaaaagccg gaagaagttt ataccgactt
    31021 tattaataca gtaaaaaatg atgaagatta tgggaatgat ccattgcaaa ataaaaatct
    31081 gactatcgtt ctttacaaac ctgattgtcc agattgtcaa aaagcagaat caaaaatcat
    31141 gcaagaaata aaagaaggat ttagttatgc tccaacatta atgcatgata ttaccataaa
    31201 taaaccagct tacgagtatc acgcagttgc tgtagatatt actgatggtg tacctgagtg
    31261 gatacaaaac attcctggtc tggctgatga gaacaatcaa tatcatacgc caacagttgc
    31321 agtagtaaca ccaaagaaag ttaatggcac actatactgg gatgtaatgg gaatgacaac
    31381 aaatacaact gatgaaatga ttgaaagtac aactaaatat tatatggatt tgggagcata
    31441 taaattatat caacagtcag aacctgttat ggacgatcaa tccaaagtta aatagttctt
    31501 tagggcgtaa gccctatttt tttaaaatta agtgataaca aataaatgat ttctgaaaca
    31561 aacgttctta ctcaactact cgaaacacac aagctaatta actagattat aaaggagaat
    31621 gcgtaatgaa agaaatcagt gcaaatgaag ctcatattcc gcaaaagaat caggttggta
    31681 tatataaggt gcttcatggt gaaggaattt ataaaataca gttacttacc ggttacaata
    31741 acggtgcaaa tgggaagtgg ttaaaggcaa aaagtattcg tgaacttaaa actgaagtag
    31801 aaaaaataag acttgggtta acaagggaag aaataagtag actgagtgaa aattcttctt
    31861 ttgtaatcca aaaagaaaag gagaaacatc tacttcatcg ggaactaata attgaaaaat
    31921 actacgatgt tgatttacct gatcagttaa gaattattga cgaattttca gagaaaaagt
    31981 ggaaatctga aatataccca agcttaattc aatacaaggc atcaggaaag cccatgtata
    32041 actacacagg ctttttaaac tacacacaat ataaatatgc ctttcgcaaa ttaaaaggca
    32101 ttaaacaagc tgacattgcc ctgtatgaga tgaaaaaaat ggatagattg aaattgattc
    32161 aagagttagt gcaaaataca tggaatcaag cgattgtcga actagaaaaa gaaaatggga
    32221 aaattttaac taatgaatat ctgatcgtaa atgaaattga agaattaatt ggtttatttt
    32281 catgtgaaaa tggtgaagtc ttcttgaagt catcaccaat ctattcaatt ttggacagtc
    32341 cgtatcagtt tatacaggta aggagatgag ttacatgtct agcttgaaag atgactatat
    32401 ccaagcaaaa caagagtatg aaaaaataca gatgttaaaa tctgagaaag tcgaattatt
    32461 tttaacacaa aaattagcag aggatcatat tgctaactta gatgctaaaa ctttagagga
    32521 atatgatcag cagtttaatc agacacatca caatatcatt ttgcttgttg atcaggcaaa
    32581 gagaagactt gaaaaagcgg aatatgaact attagatcaa ggaattatat gcttggataa
    32641 agggattcga agatattata gtgcagagtt aaaaaagcaa aatgtagaca gacatgcgtt
    32701 aaaacatgaa atcttagaat tgataatgca acgaatttga agggatagaa agtaaatgtc
    32761 acaaaacatt agtgctgtaa aacaattaat agaaaaagat ttagagacga cagttcagag
    32821 gttagccgaa cgaatccatt ttgcaaatta tcaaaccatc tctaattggt caagcaggaa
    32881 cagaaatccg gaagaaattc caatgaaatt tttttatcga atcggaaaag aatatggtgt
    32941 tgatccgata aaaattttcg aaaagaccat gatttattac tatcatggaa cacctaacga
    33001 agtgttggaa catcttttgg aagaagcaga agaaaaatca tcaatattag agatatcaca
    33061 aaatgacaaa gctattcaag tgaaatgtgt tgatccaaca ggaaagatta ttgttggatt
    33121 tgatattgaa agaaaatcta aaatagattt tattactcaa ttaacaaaga taaataattt
    33181 aaatatttta acacttagac cagatcatga acagttgttt atgagtttta aaaaacaatt
    33241 gaaagaaata caaaaaagat ataacaaggt gttttctaat gcgtgaaaag ctaaaagaaa
    33301 tcagagtgtt ttggataatt aacaaagaat ttattatctt gtatacactt ggatttatca
    33361 gcatgatctt gttttggtgg ttatatttaa aaaagtaatt taaaagtgtc aaggagattt
    33421 ttatccagag aaggatgtgg aatttgcatg tggaataata aattaaaaac aggcttgtta
    33481 ttaatagtta tttcctgcgc tatgatggtt ggcatgataa tacaaaggga acaatcttat
    33541 tttgaagttt ctgctaacaa tgtaatagaa agatgccatt atggtcaaca tttttggact
    33601 ggggaagtca gagaaaatat agatcgtgag tatgttcaaa gaatcgtttg ggatgcatat
    33661 tctatcaaag attatcctaa aagtttaacg agtagattat tttacagcga aaaagataat
    33721 caaaaactct cgaatttgat gatgaaaaaa gtaaggaaac ttgcacaaaa ctatttggaa
    33781 gaaaaagaag gtaatagtcc aattgacaaa aattgatgac actgcaaaaa aatatagagt
    33841 tcgaattagc ttttttcgga gcattgtttt ttaggtgaac tatatttcat cactaaaatc
    33901 agagataact aacttgaaac atggtcagat gtgttacttg aagtctctga ataatcaatg
    33961 ttatctagta gaattaagca actaaaaaag aaagaagtaa gaaaatatgg catacgaaaa
    34021 tttacaatta gtagtcgcag aagttagtgg ggatatatac cttacaaaag taaaagatgg
    34081 aattatggac acgaattata ggagagttgc tacagatgat gtgctaagct catcgacaga
    34141 atggtttcga aaaaatgaag aatctgctgt tcactttgaa ggaattgatg gaaacattca
    34201 tagtttgttc tatacaaaag acaaagaaaa agcaaaaaaa attcttgata ttttgaaagg
    34261 aaggtagtta attaaccaat gatataagag aatgtgagac caagtaaata cttggtctct
    34321 ataattttag ccactagtca attaaatagc taaacaattt taataataat aaaatatgac
    34381 tgcttcataa ttaagaagtg cagaattttt ctaagaacga tatttccttg ttgaaatgag
    34441 tattgctaac aatttgatca cgaacagcat cgttacaaat gttgtatcaa tatttgatag
    34501 attacatatt ctattaaaaa agaaaagaaa attattgata ttatacacat tagtgtgtat
    34561 aataaaagag agggagtgat tagaactttg agcaattcac taattgttta ataatagaac
    34621 gattcctaag aagttcattg ttcattggca aattgcttta tcagattgaa agttggcgaa
    34681 taaggtttgt agaataacac cttttcaagc aaagcgattt ttgaaagcta tgaaatttgt
    34741 ttaataaaaa aattcaccag ataatctgat agagactaac ccaattacta acagttgtaa
    34801 ttggtgttag cttacaaatt aattattgtg tttagtcaat tacaaattgt gaaacggcac
    34861 taataattgg aaaggaaaaa tgaaatgtct aaagatttac gagagcttaa attgcgaaaa
    34921 aagcaatata atcaatattt ttctagtttg ttgaataaac atattgaaga aggacttgat
    34981 catgattttg gaaaagatga atattgttta tattgcgaac gttacaatga aaaaatgtct
    35041 gaatatctac agctgaaaaa agagatacag aaattagaga aaaaagtaca tgcttggaag
    35101 tataaagatt ccaggtctgg ttatgttgaa tctcaaaata agccaagaaa aaagaagcaa
    35161 gcgtatgttc taaagatggt aaatggggaa gagttaattg tattagcgta cagtgaagaa
    35221 gaggttcgag aactcgttga aacacctgtc aggaaaaagg ttgaaacaat aaatgagata
    35281 gacgacagcc aatacaatag catctatatt gaaacaagtc atggtggata taagaaatta
    35341 gcaactttcc ttagaagttc taaagttggg ataatttcta aaaaagacat aagtgctgtt
    35401 accgatagtt tttgtacttt atctgttcct aaagcttatt tccaatacga tagtaaaaga
    35461 tttgaacgct tagagaaatt ctgtagaccg tatactgtat tgaaaactaa aaaatttaat
    35521 aaaactaaaa gagtttcgat acagttaccg actggtcaaa tctcattagg tgaaaaagac
    35581 tatctgataa aagtaggaaa agagttaatt ataaaagtac cggaacactt atttaaagaa
    35641 aattttgaga tacgtgaaac cttggtatgg aaagaagaaa aatagtttgg aagacataga
    35701 atggtataag gggaaattga aatggcagta aataaaatat tgcaggtatt aatcatagac
    35761 gaatttggcg gaggagaatg gtttgatgtg cagtggatag atgtcaataa agtagacaca
    35821 aaaagagaag atacttaaag actttcaaaa taaagagcct ccttttcagt tggtgtaagc
    35881 atatctaatg ttccatgagg acgtttgatg ttatagaatc cttcaatgta agtgaaacaa
    35941 gctaaacgta cttcttcgat cgaatggaat gaacggcggt ttaattcttc ttttttcata
    36001 tatttaaaga aagcttctgt gacggcgtta tcccacggat aacctggttt agaaagagaa
    36061 tgaacaatcg gatgattctc tataaacttt ctaaactcaa aagaggtata ttgagaccct
    36121 tgatctgtat gaaaaagaac aggactcatt aaatttcgag aatgaagtgc tttatctaat
    36181 gttttaatgg ctagatttgt atcaatcgtt gatcctacag tccaagcaat tacttttcta
    36241 gaaaataagt ctaaaatgac acacaaatat gcaaattttt tcttaccgat aggaatataa
    36301 gaaaaatcac tagtccaaac ttgatttggc gcaggtgggt tgaaagcttg ttttagatga
    36361 tttgggcgtg tcaaagaaac cttgtttttc ttttttttga aaacaggttt tactgtagac
    36421 atttttggta ggttcattga tttcatcaag cgataaactc ggccaatact aatcaaaatg
    36481 ccataatcac gcaaaaggat ccgacgaatc tttgcagcat caagacgttt ttgagaagaa
    36541 gtataaattt ctaagatcac ttttcttagg tgttgatttt cttttgttct tggagctact
    36601 ttcccagaaa aacgtttgta atacgtagaa cgattgactt tgagtacacg acagagtgta
    36661 acaatactgt gttcatatcg aagaatgtgt atagctttta atcgttcgtc gagtgaggcg
    36721 tgaatatggc aatcgctttt tttaggatta aattttcctc ctcgagttga gcatttcttt
    36781 tttgtagctg tttgatctga cgagccgtaa ggatcgtgtc atcgtcaatt tttacttcgg
    36841 aatattgttt gatccagcga gcgatcgaag aaagagcgat accgtattcc ttagataaag
    36901 cattttgggt tttcccactt tcgtaaagtt taacaattga ttttttaaat tcttcatcaa
    36961 atttacgcgg tgattttctt ttgtctccca taataaaaag tcctttcaaa atttgtgtct
    37021 atttatttaa taatatatca aaaaaagtgt ccactttatt aggatacatg cacagtccat
    37081 agactttgaa gaagaagtcg tagttataga aaatatttac ggcgaagtat actcatattc
    37141 gcttgaaggt gagaatgttg aggatctacg gttgattggt gagtagaaaa aagcagaaat
    37201 atcaaaatgg agataaaaat taatagcatg taaagatagt catgcttaca tgctttatcc
    37261 aaaatggtat gcagacaaaa atatagctga aataaacgat tatctaacta gtataaagga
    37321 acgcaatgca attaattaac aatagacgaa aaatgaaatt aaaggagata ggaaaatggt
    37381 agaagttgaa tttggatcaa aagaatggtt tgaagagtta aactcaatga ttaagttccc
    37441 agaaaagttt actttttact acaataaaag agacaatatc attcaagtag ctttacttga
    37501 aactaaggga agattagaaa atactcgatg gagtatgata aaaccatcgg cacttagatt
    37561 tcatcctaaa gacttgacag cggttgtagc ggtaaaagag gttcaagact cacaagaata
    37621 ttatgaagta gtaaaaaatc tgaaggatat ttggtgtaaa taattctaaa ttgaccagtt
    37681 atttggtcaa aaatagtatg tgattatttc atccaatgtg gcatcataat tggtatggaa
    37741 ggggataaat tatttgaaag aaatagaagg tattaagaaa agaaatcagg aaagattgga
    37801 actgttaaat gaaaatagaa atagaatgtt tagttattat gaatctatga ggtgtttaaa
    37861 taaatctaga agtgaaatta tagaatcatc aaagtataaa caattgatgg aagagagagt
    37921 ttctctcatc caagaaatag aaacagagtc tatttttcca gatcattctc ctaaagtccg
    37981 attttataat taaacattaa caaatgaaac gatgctaaat aggcatcgtt ttttatctat
    38041 ttataaaatt aatttattta caaaatgtgt atcggtatgt atggtgaatt tgtagataag
    38101 ttgagaaagg gtgatcttaa atgaaattag aaggtagctt acaaaataga atgatggaag
    38161 gcagtacaac aaacaaggag attgtagttg gaatgggagc aaccgagctt ctatataccg
    38221 acagaaatcc ttatacagta attgaagtaa aaagcaaaaa cagaattttg gttcaagcgg
    38281 atggagcaat caataaagct acttttccag atcaagagtg ggaatatagc agaaatcctg
    38341 aaggtcaaat attggagtta gttaagacaa aaaatggatg gaaagtgctt aaagaagata
    38401 ttcattttta tataggtgat agagaaaaat actacgatcc tagtttttaa acaacctgaa
    38461 gatgagcgag gttttatcct cgctcagaaa ggagtatgga ggatgttcag tcaagaagaa
    38521 atcatcagca aatcgcaacc ggataaaaat ggaactataa tcatgaagaa aaaaatccct
    38581 tgctatcgtt gtagcggatc aggtcagtac aagtttatgt ctatggggaa aattacctat
    38641 ggcacatgtt ttaagtgcga tggtgatgga tttcttgttg taactgaaaa atcctttacg
    38701 ccagaatata gaactaagct aaatgtttta aataaaaaag agctgatcga aaaataagga
    38761 tcgcaagaga gaaagaatct cagaaaaatt ttgatcgtta tggctttgaa aagggttata
    38821 tttacgtggt tatagagaaa aatagctata cgatcaaaga tgaactgcat catcaaggag
    38881 ctacttatga tcctttatta ggttggtttt tttaaggaaa aacaagaaca atacaaaact
    38941 caaaaagttt ttgtagaaga attttctgta atcaatgatg aaggaattat tgagttgaag
    39001 ctagaagaat acagcaaaag acaacaggag attttaagtg cgaagcgtag agaagagggt
    39061 gagtttattg gtacaatcgc tcaacgattg gaattggatt tgaaacttac caataccgtc
    39121 accatagata cacaatgggg caatgtttac ctgcatttat ttgaagatca aaaccataat
    39181 tcattgattt ggaagacaaa taaatttctt tattatgatg aatgggatgt tggagaaata
    39241 agaaaagtta aggcgacaat aaaagcgcat gactactaca gagataccaa gcagacttgg
    39301 attaaaaatg ttaaaattca aaaaaatgag taagaaggga taaaagtatg aaaagagaag
    39361 aatacaaaca aagattaaac gaactgcttg aagaggacga aacattaact catggttcgc
    39421 cggatgaaat tctatacatg attgataata tggtaatttt tggtggatat gaacttggta
    39481 ataggtcagt tgatcataat attttagaat ttgatgatgt atcatgggaa gaaattcttg
    39541 attggggaat tttggcagta cctgaaacta aaacctatat tagtgacact atggttcctt
    39601 tttttgagga attggattat aaaagacttc ccaaaaatga aaatcatata ctgggaggaa
    39661 actaacaatg ttaagtgact tacttatttt actgatctat ctaggtggaa ctgaaattat
    39721 tgcgtatttt ttgcttgtaa ttggtgaata tggtatttcc agtgcaatca ctcaaaggtt
    39781 tagattacca gtaagtgaac taaaaaaatg atgagattat cagcatatct tttaatgttt
    39841 ttgaatgtgg tcatgttaat ctttgtagta atcggtgtga ctttcaattt aacggaatag
    39901 agggaaatct ataatggaaa aaagagaaaa gaatgaacat attttttgag tcaatattgt
    39961 tgatggtttt tgcgatcagc gcttaccaga aatctaaaaa agagtaatga ctgtttagtc
    40021 atcactcttt ttttgtaggg aaattgaaat atgtggagct tgtattggtt tagttaggat
    40081 tgcttggcta taaggagcta gttctttatc aaaaccatca aagtaggttt tcagtagcga
    40141 tttaactgat cgctcatctt gtttgatttg gataatttca gcatccaaat cggttgtgag
    40201 gtcaggagct tttgaagcga ttacctgatt ttttttatct tccaactttc ttaggctgtt
    40261 taacaagttg taaaaataat cattttgact gttcattcca acgtgagatg caattttaac
    40321 agaagtttca tccgtattgg catcaagtat ttcatcaaaa gaactcattg gaatatcaag
    40381 tagtaacgag gatgtttcct ttacagcatt tttagttttt ccatcttttt tgctcttaac
    40441 ttgagatatt tttggatcta gtttaggtaa atcacttgtt ttatcagtca tttattagct
    40501 tttcctttct attaatttat gttattatat tatcataata acataaaaaa agtacacttt
    40561 ttgggggcgt agttattgaa aaaatctaag agaaagattg ccacgttgct tacgttaatg
    40621 gtgcagttta cagggattgc aatgagtgta atcacaataa atccgcaagt gttacatgct
    40681 gatgggatag ctacttatga tgcacaattt aactgtacta ctgaaggtag tgggtcagga
    40741 acagatagcg atggaaacga tactggaagt ggcgctgtaa caggtgatgt agccaaaaat
    40801 attgaagata tttacaaata catgaatgga gaatatggct tatcaggtgc tagtattgca
    40861 ggtattttag gtaactggaa acaagaatca ggtattgatc ctcgaacagt agaagggtat
    40921 tacggcgatg ggcagagaga agttgacttc atcaaacaat ttactgatgg tctaatgggt
    40981 attggctatg gtcaatggac agccgggcga catacagcat tagttgattg gtctaagaaa
    41041 aataatggcg gttcttggtg gactacagaa gcccaattgg actttatgtt caaaggtgat
    41101 gggggattcg taggaatatt aaaagattat gctttaaatg cttctgatga tgtagtagaa
    41161 aatgcagtaa cttttcataa aaactgggaa atttcagctg attccgaatc agctgtaata
    41221 gctgtacgtg gtgaaaatgc aaaagaaatc tggaagtaca tgaaaagcaa gggtatggac
    41281 ggtgcaaaag atgaaagtaa gatcaataaa atatccggag gttcaagtac cggagatgga
    41341 tcgaataatt catcaagttc agcatcagga acagtagaag atgcctgtgc aactacggat
    41401 gatgctaatt ctggttctgg atcaattggg aaagaaggaa aaattaacgg taaaaaaggt
    41461 caagtaatag caaatatgac ttatgaagaa gcattaaaaa aatatgggaa agcaatcacg
    41521 ctaccaaaat ttaaagaacc agactggtca acatcacctt ttcaaggtga attacaagga
    41581 caatgtactg aattaacatg ggcttatatg agtcaattat atgatggaaa tcaaccaact
    41641 ttgggaaatg gcggttacgt ttgggaaagc tacaaatcgg caggagcaaa ggtgacgaac
    41701 aaaccaattg taggttatgc gtttagtgct ttagatggat ggttatgggc tgttggatca
    41761 gcaggacata caggtgtagt tatagcggtg tttgacgatg gatcattttt gtgcgctaat
    41821 ttcaatgttc cacctaccca agcaccaaca agaggtgttt cagtaacttt gattgatggt
    41881 gtggatgggt cagataatgt gcatttcttt agcggtgtag gcaaagctaa atttgaaatt
    41941 gaaaaataaa aatttgaatg gagggcgaca gagatgaata gtaaaacgaa agatgcaatt
    42001 atcatttctt tgctttctgg tatgctatta gcaatgcttg ttggaattct atatttcaat
    42061 aagcagaatc aagacataga agctgaaaag cacagtgtgc aaattgaaaa tgaattgacc
    42121 ttctttaaag aggttaaacc aaatgaagtg gatggattgc aaagagattt agaaaatgat
    42181 attcaagaga tgtttaaagg atcagaccga agcaaatttg cgaatcagtc aggtgaagaa
    42241 tttttgcgaa agttatttat tgtgacaggt aagccaccag ttactaacga gagtagcgag
    42301 caagaaaaaa tagaatacta taaacctttt acttttaagc taacaaactt tggaattaag
    42361 acatctagta gtgggttgta tgaagcattg actgatttcg aagttcaata caacggaaaa
    42421 aaagtgtatg ataccacatt aaccatcaca ctaaataatg ataagaaagt gactgggggt
    42481 gctttttatg caaaatcatg aaattactac tgctaaaaag gcgatcggaa tactttccat
    42541 aattttgatc ctttgttgtg tggtactatt tcaaaagaaa aatgcttatg ataataatgt
    42601 caaagaattg gaagacctta aaagtaaaca aatcgaagta gtcaagaata accagaaagt
    42661 gaaagctatg caagaagccc aatacaaaga attgggaaat gctgaaatca aggaacaaag
    42721 taggaaattc tttaatttat tttacaactg gaattcatgg gaaaaatata cgaagaatat
    42781 gaaggaaata caaaaagaat ttcctcagat agtaaagtct agtcctgttg atatctctgg
    42841 cacaatggta ggtactggta atagtccaat atctagctat actgctgatt actatacaac
    42901 aaatcaaaaa ggtaaaatgg ttgaattgat tacacagaca agaaatactc caacaacagc
    42961 aacaacaaca atttggcgtg gagtaacaag cataggagaa aatcaattgt tttacttgga
    43021 ggatttagat agctatgtaa gagcagagta aaataaaaaa agagctaacg gtaccaacgt
    43081 tagctctttg tgctgacgat gataacaaaa cctaccgaaa aaggattgtt tcatcacaaa
    43141 cacaaacaga aaagaattga taaaaaaccc gaacctcaga atttttatta atcaattcat
    43201 tttgattata ataaattgag ctgaacatta caatacgtat atgttgtaaa acgtaaaaaa
    43261 agtcaaaagt aaatactttc ttaaaaagtc aaataaaact attaaagaca atatatatgg
    43321 tttatttgac ataagaaaat ggtctagatg ataattagaa tatagaattg tagacttaaa
    43381 taaatcataa aaaaccttct atgataagtt atagctctta attttaagca gttttagata
    43441 taagaaagta aagtggggga gaataataat ggcagatcgt aaattagaaa agttattaga
    43501 agaaacgtgg gatccaaaag aatttagtga attttttatg gagaattttg aaactgactt
    43561 agcggtgatt gttaaagatg ctcttagaga gcaagggtat cctgaaactg caaattatat
    43621 aaacattaat tttactttgt atactgaaaa tagaggaact tgggattttt gggctaactt
    43681 agcaaataag gagttaagtg ataaatcaga tacaggaatt cgtaactttt ttgaatcaaa
    43741 cagagatgat tatatgtacg ctaacaatca aaataaattg aactttagag tagagtttga
    43801 tgaaacacct gaagaattta tagaaagaca accgcctaaa gaaaatgtag caaaggtatt
    43861 ggaagatcgt tggaattcag atgaaatagt ttcaacaatt agcgaacaag acggacaata
    43921 tgaaccgcta gtagaagcgg ttagagaaga gctaagactg aataagtttc ctgatgttca
    43981 aaatattgat gtttctcaaa ttgaaataaa cgtcaatatc accaatagac ttgattatga
    44041 tagttgggca gatatagcat tagaaaaata tatttactca acgttaaaag agtttatcga
    44101 aaatagaatg aatatcatgt atctacagca tccacagtat ttaaactttg gtgttgaaat
    44161 agctacacct cttgaagaat ggaaaatgga gcaagggctt gactagcctt tgctccattt
    44221 ttttatttta caaatgaata cagttcttcg tctttaccaa gaatgattgt ttgcccaact
    44281 ttgagattat caggtgatcc ttcagcggtt tgtaaggaga acgttccatt gaacttatcg
    44341 gataccgtta taggtagctt taattctgat ccttcctcat ctttgagaat tgctgttttc
    44401 cgttcatcaa aataaacagt ctttattaga taatttttag tagtggtttc atcagatttg
    44461 tctttgtttt tagtagtact aatttcttta gaattagatg tattttctat gactatttcg
    44521 tttttggtat ttgcgcttgt atttatttga ttgaaaattt taatcgacaa gatcccacta
    44581 agaatagcca acatagaaac aagtgtaatt aatactgata atttcctttt tttgttcatt
    44641 tcttcttaac ttcctttttt gttttgatta gttagatatt ccttatctaa taagcctttt
    44701 taggctatta aatgaagata aaccccaaaa aattattgca ataatgatag tgcaatggta
    44761 ctattattat aaaataaatt ttgcgaaagg ggtaggtcta aatcgcaaat aaaaagaaat
    44821 tgaacctaga caaatacaac ttgtataccg caagggaagc aagtctaatt ttgggtaaag
    44881 gtagaaatta tgttacaaac ataatcaact attatccaaa tcgtttgcct aaaggttcgt
    44941 tcaagaaagt gggtgacaaa acctatatta taacggaaga aggaatagaa gcattgcgat
    45001 cacaaacaga aaataaaaac aaaaagaagg aatgaatatg aagaaaaatt cgcttgctat
    45061 ggttttattt gcatcagctg tacttgcggg gttatcagta ccagtaacct cacatgctga
    45121 tgaatggact gcaaacacac cggatcaaat ccaaattaca acaggacaat caagttatgc
    45181 gttcaaaaaa ggagatacta tttggtcaac tgtagagaag cttaaaagct taggttacaa
    45241 gactactgtt gctgagtttg ccgaattgaa caatgttgat ttggcaaaag gagaacaacg
    45301 gactatcccg attggacgaa ctttcaacat caataaagat acattaacag ttaaggaagc
    45361 agatggcgaa gtagttagtc aaactgttca aaaagataaa cctgatcctg ttgaaaatac
    45421 ggagcaagag aacaaaaaat ccgagactac aaaagaatcg gaaccaacag caaacacttc
    45481 tgataagaca gaaacaagtg cttcaagtgt tgatggagca agttcatcta atacaaataa
    45541 ttctacaagt gagaaagaag catcacaggg aacaagtgaa agtagcacta cggaagctga
    45601 tactggaaca tcaacaaata actcacaatc aacatcaaat gatgcaaatg aatcaaccag
    45661 tacatcttct agtgatcaat cacaaacagg ttcaagtgat gaaactgaca gttctacttc
    45721 accaagtaac cccgaacaac caactaatgt tgtgctaggc gttccattta ttagtcaagg
    45781 taacacaatg ctttgcgaag gaacatcttt gctagaagcg ttacattata aaggtgttgc
    45841 aacaaatcaa gatttaatga cttttgttaa atcaatgcca ctatctcctg ataataatcc
    45901 aaataatggt ttttctggtg aatggagaca caatgtggat ggaacttatc aaggtatgaa
    45961 tccggctcct gtagtttcat ggggtgttgc taatggcgga aatgttgtag atatttctgg
    46021 ttcatcggta caaggattaa aagatgagat tgtaaatgga aatcctgtag tagcgtgggt
    46081 aacttatgcc tttgaacctg ctcaacataa atcaatgttt tggggcgatg ctatttggaa
    46141 tcgacatgta gtagccgttg atggatttaa agacggtttt taccatgttg tcgatcctgt
    46201 gtttggggca agttggatta atgcaggatc atttgaaaca gcatataacg tatctcaatt
    46261 agcggtttcg gtacgttaat aaaagagatc gtttatatac taagtagtta agtatagtct
    46321 ttatttgtcc ttgcttaact acttagaaaa cttgttgctc atggttggta gaactaaaag
    46381 gggtgatgct atgagtacta gagcgttgaa acagcttgct ttcaacgaaa aaagtgatta
    46441 ataggtgtgg tgattttaac ttaggaatgg gcgcgaaggt cattaccaca ccgacatatc
    46501 gtggctcatt ttcttcgcct aactggtata aagaaggagt aacgagagat gaagcatgaa
    46561 aaaacgataa ttttaaaatc aaaaataatt tattgttttg cgactttagt aatggtgtta
    46621 tctacgctta taatgccttt ttcagcaata agagctgaag cagatgtcac aaaaccgaca
    46681 agtgcaaaat ttacagaaac tggcgtaaaa acagacggtt atattagggt taatatgccg
    46741 aatcatccaa atgaatggat gatttcaagt caatttaaag attctcatgg aaatattgga
    46801 tattgtatgg attcagaatt gccatcccca acaggaagtg gagccggaag cttgaagtac
    46861 aagggagctg gcagtgatga gttctatcgt atgtttaaag gtggttttcc atcaaaaaca
    46921 gcaaaggaac tgggtgcagg aaatgataca gaagcatggt atgcaacaca attagtttca
    46981 tgggttttag ccggaaactt taaagtttca cagattgtat ggtcacatcc aaaccatacc
    47041 gccgcagaaa cagctcgtgt aaaaaaagca tttgaaaaaa tttatgacta tgctaagaat
    47101 ggaaaagata cgcccaacac tgagttttct atcacagctt ctaagacagc tgacgaagga
    47161 aaatatcaca ctttcacata taaaacagca tcaaataaga ctggtaatgc gaagctcact
    47221 tttacatcag caaaaccagc tggtatgaag atttatgatg cagatggtaa agaaattact
    47281 aacaacactg tgaaattaaa ttcaagtttt actattaaag tacctgtgac tacaccaagt
    47341 ggtactttgt catttaaagg tactgctaat gttagcacaa ccaatccttt tacttttgat
    47401 ggacgaggtg tgtatcaaga tgccgtagtt atgatcacta cttcagaaac aaaggactct
    47461 aaatctttga gtgctaaatg gacaagagct gttggtcagg taacagttaa gaaagttgat
    47521 caagatggaa atgctttaaa aggagcggag tttactctaa ctgacaataa caatgctaaa
    47581 tctgttaaga caaccggaga tgatgggtta gctacgtttg atttgcaaca aggcttaaca
    47641 tacagcttag aagaaacaaa agttcctgaa ggttatcaag gttcatttaa aaaggatggt
    47701 attactgtta ataccgatgg acaagaattt agttacactg ctacgaacac aattaaaaaa
    47761 ggtaaagtaa aagttaaaaa ggttgaccaa gatggaaaac cattagctaa tgctgaattt
    47821 accttgacgg atgattcatc ccaaacacaa gcagaaaaaa cagatgaaaa cggacttgca
    47881 agcttcgata ttgtagcaaa caagacctac agccttgaag agaccggagt accaactggt
    47941 tatacaggcg tattcaagca agaaggaatt accgttaaag atgatggaca aacttttgaa
    48001 tatacagcta caaacacttt gaaaaaaggt ttagtaaaag ttcataaagt cgatcaagat
    48061 ggaaaacctt tggcaaatgc agagttcact ttaaccgatg aaaaaggtgc aacacaagta
    48121 gaaaaaacag atgaaaatgg acttgcaagc ttcaatattg tagcaaacaa gacgtatagc
    48181 ctagaagaaa ctggagtacc agtaggttac acaggtgcat ttaagcaaga gggtatcacc
    48241 gttaaagacg atgggcaaac ttttgaatat acagctacaa ataatttgaa aaaaggtgta
    48301 gtaaaagttc ataaagtcga tcaagatgga aaacctttag cagatgcaga attcacctta
    48361 acggatgaaa aaggtgtaaa agaagttaaa aagacggatg caactggtct ggctacgttc
    48421 aacattattg cgaacaaaac ttacaactta gaagaaacaa aagtaccaac aggttacaca
    48481 ggcgcattca aacaagaagg aattaccgtc aaagatgatg gacaagtctt tgaatatact
    48541 gctaaaaaca ctttgaaaaa aggtgtgatc aaagttcata aagtggatga tcaagggaaa
    48601 gcattagcaa acgcagaatt cactttgaca gacgacaaag gtgtaaaagt tgtacaaaaa
    48661 acggacagca atggcttggc aacatttaat attgttgcca acaaaacgta caaaatcgaa
    48721 gaaacacaaa accctaatgg ttatacaggg aaatggtcta aagaaggcat caccgttgct
    48781 gacgatgaac aaacctttga atatacagta attaatcacc ttgtacctga aacagtaaga
    48841 acaggcggaa tcggtacatc agccttcctt cttgtatttc taattgcagg attgagtatg
    48901 gtgctactgt attatcgaga acagcacaaa gcaaaataaa aaagtgttaa tggaaaagaa
    48961 gtaaatatga aaaaagaagg ctgacgtatg aaatcaaaaa aaattttttc aaatttaatg
    49021 ctatttggta ctttgctagg agtgggattg ggcgcaagct caatctcttc tagccttgga
    49081 ctaggggggc aagtagcata tgcacaacaa aactataatt atcaaggaac tggaacgatt
    49141 catatccata aagtaacaaa tactaatggg gatgggaacc ctgtctcaga tattgataac
    49201 aaagggaaag aaatagcttt accaaatggt gtaaaacctc ttgaaggcgt tgagttctct
    49261 tatgtgaaac ttactaaaga tgaagctaaa tccttgggat ggacggatag tgatactaca
    49321 aaaggtcaac atattgatga tgctaaagca cgattattac taaacaaaaa atacagtgaa
    49381 gtaagtagaa tgaagactga ttcacaaggg aatatagaca tacctataca agtaaataca
    49441 gacgatttaa cagataactt gtatttgatt gttgaaacag gaacagtcaa aggtgtagtt
    49501 gaagaagcgt atcctatgat tgttaacgta cctgaactaa acggatcaga tgatcaatac
    49561 tttaattatg atgtacatgt ctatcctaaa aactatttcc aattaaactc agcagagctt
    49621 gataaaaaag acgaagatgg aaaggctctt gccggaactg tttttgaatt gcgaaaagat
    49681 gatggatctg atcaggttgt tggaacttat aaaacagaca gcacaggtaa attaactgta
    49741 gatggtttgg aattaggtga ctattatttc ctagaaaccg acttgggaac agatgctaat
    49801 aaaacaacct atttactaga tggtaaaaaa ttcaaattta gcgttaaacg tggggagact
    49861 gctaaagtag ttcttgatgt taccaactac aaacgacctg taattactaa ggtaattgat
    49921 aaagaaaccg ttgatactgg cgaagatgtt caatatacat tgaaaccatc aattcctggt
    49981 aatattggta attacgaaaa atatgttgtc aaagatgttc taggtgcaga gttaacttat
    50041 gattcatcag aagtgatcga aggaatgact ttagatactg attacagtat cgaacgtgac
    50101 ggacaaacga caaactatgt ctttactgaa tcaggtatgg ataagcttca gaaacaattt
    50161 gatgcagggg atcgcttctt tacaattttg atgaacgctc atactaatag taaagctaaa
    50221 atggatacac cacttaagaa tgatgtttca acgatctaca acaatggata tgaacccgaa
    50281 gacgaaacac cagttgatca cgcttataca atcattggtg atcaccaatt cattaaaaca
    50341 gatggagata caagcaaagc attgagtgga gcaaaatttg tagtgaagaa caaaattgat
    50401 ccgaataaag ttacttacct aaaacaagat ccaacaacga aagctgttac ttggattgca
    50461 gataaatcta aagctactga attcgtttcc ggatcagacg gtaagttcca aattgaaggc
    50521 ttgcaatata cctatgatgt agattttgat aaacaaggta atccaacaat cacagataaa
    50581 aaaatcaatg attatgctct tgaagaagtt gttgctcctt caggatatac accaactaga
    50641 aatgatgttc catttgtggt atcgaatgat gatgcttcta gcgaagtaac agacatcaag
    50701 aaccttaaaa cacctgaaac agttcgtaca ggtggaattg gaacagtagt atttctagtg
    50761 gttgcaggtg tggcaggaac agggctatat gttgtctaca aacgagaaca acacaaagct
    50821 taatgaaata aaattcccta gagtgttttt ggctctaggg tttcttttta aaaagtgtgt
    50881 gaaaagtagg tatgagcatg aaaaagaaga atatcaagtt gatcatctta aaaattggga
    50941 tgttgctatg tttaggagta ggtctatatc ctgtaattac tcaggcttat acctactacc
    51001 aaactcaaca aacactaaac tcctataagg aaaaatatga tgaagcttct gaaaaggaga
    51061 atgaagaagt cttcaagaga tattcagaag ttaataggaa acgaaaagac acagaacaaa
    51121 cctcagtcac gattcctgaa gataataaca atattccgtc aaatggaaat cttcagaagg
    51181 ataaattagg tgaattagtg ggtgaaattc ttataccaaa gattaatgtg gatttgccaa
    51241 tttatgatgg tgtaaacgat tttcaactat cagcaggagc aggaatatta accggaactg
    51301 attatccaac tggtgggatt ggaaaccatt ctgttatatc agctcattcg ggcattccta
    51361 ataaaaaata ttttacagat ttagagttgc tcaaacttgg tgataaattt tttattgata
    51421 tttcagggaa aaagcttgct tatcaagtag atcggataaa aacaatccta ccaaccgaat
    51481 ttgaggattt gcttcctgtg gcaggaaaag attatgtaac acttatgaca tgtacacctg
    51541 ttggagataa ttctcatagg ttactcgtta gaggggttag cataccttac caaaaaagta
    51601 ttgttgagaa agaaaagaag aaagaagatt taaaggtaaa ggcgcagatg aactggtatc
    51661 ctatcgtagt tgtaggggtt ttattaataa tagctgtgat agtcattatt aaaaaaatag
    51721 tagcaaagag acgaggaatg aaaaatgaag aataaaatac tacttttttt gactcttatt
    51781 tgcactttgg ttttaacaat ttatcctgca aatcagctct atcatcaatt caaaacaaaa
    51841 gagaatgtgg cggaaatcca agagaaaatt gagaaaaaac aaactccttc aaaagaggaa
    51901 gtggcaaaaa aagtcgaaca aataaaatcg aacagcggta accaaggatt agacaatact
    51961 cagaaaacag acggcggatc atttgatatt ggtgaagctg aaggaaaggt caatccttac
    52021 acaggagatg ttttttcaaa aaatcaaacg aaagattttg aaccatctac ctattcagga
    52081 gcggttggtt atgtctatat tccaaaaata aatgtgctag agcttttgtt tctaggcgct
    52141 tcacaggaac atttatatag aggtgtggca cagatgagtg ctactagtct acctttatca
    52201 ggtaaaggtc atcaaacagt aatagcaggt catcgtggcg gttatacgtc accacaattt
    52261 ctttatgttg atcaattagt agccggagac cacatttata tagattatct tggtgaaaaa
    52321 gcagtttata cggttttccg aacagacgtt attagaaatt acggtcagaa ttcaacactg
    52381 actgatattc ctgatttaga tatgttaaca ctttatactt gtcatccata tcctcagaac
    52441 tacgatcgtt tattagttag aagtctaaga gatgaaaaag caaaatacat cacgccacaa
    52501 gtaacagttg gcaataccgc atacgctgat gccattgata gcgagtccgt ttcagataag
    52561 acagaaactg ttacatcttc taatgattta tttgaggatg ttgatcgtgc caacaaggaa
    52621 ttagatggaa ataatgatct taatattccg gaacaaggta gcgtagagat gcaagacaga
    52681 acatttgctg aaaaggcaag ttcaatattc acaggaatta ccaataaaca tcagaaaatg
    52741 cttttagtca tttggactgt aataggagca ttagcaagtc taaaggttct tattctttta
    52801 atcaaagagt ttcgaaaagc ataatgaaga ttataaaaat tgatagagta cagtaaaaag
    52861 ggattgatac tattattgat ctctttttta ttttgaatag agctagattc acttttaaat
    52921 aaaatcaaaa ctgattttaa ttctaaagaa aaagtcaaga aaaaaataca acaaattttt
    52981 ttatcagaat atttatcaga atattattag aaaagataaa ctcaaaagct taatttaata
    53041 gggttattgg ttgtatttag aaataatgta tggaaaagct ataaagaaat agataagatg
    53101 tagaaaatct tatttgtgaa tgaaaaacaa gtcatcgtaa aatattaata ggaggtgtag
    53161 taatggtggg aatatcaaaa acagctttag actcattagc taaactattt agtagcaggt
    53221 ttgatagtgc agaatttgtt gaatatctaa gccaaaacga aagtggaaaa actctgatcg
    53281 aaacaatgaa aaaagactat ttgaatgaaa atgactttcc gacatggacg aatattgaag
    53341 atgctacatg ggatgtagtg tgtacgatat attctccttg ggtaacgatt gctgattttg
    53401 ttttagataa aattggaggg gatacatacc ctgatgccaa atctcttgct acaagtgact
    53461 ttaaaacgtt ctatggttat ctaataggtc taataggcaa tggaatagat ttgaaagtta
    53521 cgccaaaaca aacacctgaa gaaattgaaa aagaaaaagg agaggttcaa atggcttatg
    53581 caaatgacag agaaaaagta aaattattgg aaggtaaatt atctgaagaa gaacttggaa
    53641 aaatggtgca agaaatcaat gaagacagca actattttcc tgaattgaag gtatatcaaa
    53701 atgacgaaga tttctttaat gaaatgttta gtaatagtcc atgggaagca gtgagtaaaa
    53761 caaatttagg agaatggaac tttcaagatg atctagttgc atttagcaat gatgggtatt
    53821 tacgatcata ttcagaaagt gagtatagag ctgaattaaa aacgtatgaa acggatattc
    53881 tgatgagcta tttaaatcta ttagagagtg gtcagggaga gttaagagat aaagttgaag
    53941 aactattaaa agaagagcta tagggattat ttcaacataa acacaagcag aaaactacca
    54001 aacaaaacgt accatgcaaa aagtcggtta aaccggcttt ttgctctatt atgagaggag
    54061 atcaaaccat gagtgcaaca accgatatgg ctttagaagc agtaataaaa gataatacag
    54121 gaaatgtcga aaatgtagaa gactttagcg atattgcaat caatctttca gcatggattt
    54181 cagctgtagg agaagaaaat atcgaatttg agatcaagaa tatggatgac ttaaaagcaa
    54241 aatttccttt tatcgcttcg aaactaagtc aatttgccga ggataaatcg tggaatgaat
    54301 tagttagttt ttccgaaaaa atttatgata ttacgcaaga agatgagtgt gtttcatggg
    54361 aaatgaataa tcaatacaag aaaattgatg ctttcgtcaa atcacgtata gaaggaacag
    54421 cttttgagtt tgaaggtata gataccgcag aaaattggtt aaataaagaa tcctttatta
    54481 aaagtatagc ggatgatctt gaaaaacaat ggtctaagga agactatgca gagtacctaa
    54541 aaacagaaag agatacagag gtgaaagaaa aactcactga ggtatgtttg gatgaacata
    54601 ttgatcccaa tatcgtaaaa caagaagact atgaagtggt tattgataaa gttcctactg
    54661 attttgagga agttgtaaag tatatagttg aagaagcatt atcgcaatac tcttcagatg
    54721 aggaagtgga cgaagaaact gttcgtacca gagtttggca actggttttt gatgacaaag
    54781 attataaata tttgagtgat caaaactcgg ttaattggag tgtacgatta ttaattgatc
    54841 aaaatgatat tgaataaaat agagctgaaa agctctattt ttttgataaa aaaactagct
    54901 attttattaa aaataatttg gttcaaaatt tattttttta acttgataat tttttgtcat
    54961 ccttttttaa attggctata tatcaaggtt tatagtggga acaaataaaa atacatttga
    55021 caaaatttac aatatagttt attatatatt tatcttatta tgataacaca ataaaaggaa
    55081 tgatggtatg aaaaagaaga agtttagtcg atcgttgtta ggaattttgg ttttagttgc
    55141 ggttggtctt agtggcaagg gttactttca gaataacagc actcaggaac atgattttca
    55201 gatcaaaagt agtgttcagg taggtaataa agcagacagt gaatcaaaag aatttttagg
    55261 gatacgttat gatccaaaca aatatccaaa taatatcgta aacattaatg atgggaaata
    55321 tacactgaca aaagaagacc atgaagcatt atcagaaaaa ggcagtcaaa ccttttgggt
    55381 aaattatgca gatttagatc aacttggaag agcaggaaat gttgaagcgt tgatcaccta
    55441 tgatgctgta aattctcact catctaaaaa tgtggaacgt ccatctttca aaagttctgt
    55501 ccatattgca ggagaataca aggatggagt atttgatcaa gagagagata cttgggtcgg
    55561 caaagattca aacaatgaaa tcactcagct agaaggctat aaaggttatc tctataacaa
    55621 aagtcattca ttagcatggt cgctaggagg agatatggag gtacacaatt taacacttgg
    55681 aacgagagcg caaaatgttg ggaaaaatga tcagaatgga gggatggctt actcagaaac
    55741 gattgtacgt gatgcaatct ataaaaataa gaatgaaaaa gtattgtata aagttgatcc
    55801 aatttataaa gggaatgaat tggttcctcg tggttctcat gtcgaagctt tttcagttaa
    55861 cgataatgga aaaacggtaa accttaatgt ttatgtattt aacaatcaaa aaggaatcaa
    55921 catcaactat caaaatggta cttgggtaag agattagaaa ttgagaaggt gaagaattgg
    55981 aaacaataag catcaatgga ttagataaat cagttgcaaa tcgaataaaa aaaggaaatg
    56041 gctactatct tctgaagaag ggtaaatatt cagttgctag agagttagaa acaatgagta
    56101 tcgtgtatgt agctaagaat cattttgacg tagaacaggc tatttttgat ccagattctg
    56161 aaatagggaa atgtgttgaa ttatcagaga aaactgttag tacaaaaggg gcgatgatca
    56221 ttgagagaga aatgttagac gaaatcagag taatcattcc taaagatacg cagatgtgtg
    56281 aggacagcat aagtgacgat taaccaaaat cagaaagaaa ataaggagat attcagatga
    56341 gcgaaaaatc cattgaatta aaggaatata agcgaagcga tggagaaaaa tattatcctg
    56401 tagccaattt ttctaaatca gtatatctgg ttggacgtgc attggtagtt agtgcaattg
    56461 gcatatatat tttgcattgg gtaacaaagg ttgtacaact gcttgtagat accttagtaa
    56521 gtattctcgt ctcgttaaat gatgttaacg gaacagatga tttaggatta aaagagatgt
    56581 ttaaagagtt actcaatgcg ttttttccaa attatttaag ttattcatat aatgtacaaa
    56641 gcttttttgc aaagctacac ttggtagata tctctatttt agtattagaa gcaggaacgg
    56701 tattctttgt cggatatttg gttactttga tgttcaggca tcactatgga gaacaaagac
    56761 cattctttga tgatatggaa agtagaaggt tgaaaagaga agctctagaa gctatggatg
    56821 tgaagtatga tccaaacaag attataggag atgaaacgaa caacaaaaaa agaaaacgaa
    56881 acaaaacgca attagaatcc gatgtgctat caacgcttaa aaatatcaaa atttctgttc
    56941 atacaagacg tgatctaagt gtcaatgaag atattcgaga atatatcgta aaaataccgc
    57001 aaccaaaagg gaagaaagtt agagaaacag ttttacgaca aattcaagat ttagggtcga
    57061 tcctgacaag agcaacaaga gggcgagcta aatttgggga aaaagaagac gaagtttcag
    57121 gtgattactt catcttcaga gcatcagtaa acattgaaag agaacgtaag gaaagagtaa
    57181 gtaccaaaaa caaggaaacg gaaaaatcaa cagcatccat tgcagtttca aattcacaat
    57241 ttgcttttcc aatcaatctc tttgcagata actccaaaaa tatccaaaac caaaccgaaa
    57301 aagctagaaa atatgccgaa actcaaaagc aatctttaga actttatcta tcttctgctg
    57361 acgtacctgc aagtctaaca gatatgcacg ttgggaatac atctattgaa tatttgttta
    57421 ctttatctcg taccagaaaa attcaactaa gctcaatgaa agaagaaatt gaaacttact
    57481 tgcatatatc aggtgtaatc gtgcggatca acgcagggcg tattcaaatt actttaccat
    57541 taggagaaaa tgatcgtatt ccaattgatg taccatcaat gatcaatagc gtgttttaga
    57601 agacatcaca aatagaaaca gaaaatacaa actaccagtt cacaaacaga aaagtaccga
    57661 gaaaagagag gaatagaaat ggctatgaaa ttgttagaga aagtcaaaaa gagagccgaa
    57721 aaagctgaat ctattttgct agataaagaa cctaatataa agcttaatgg aaaattcagt
    57781 gtcaatatgt tgaacacaaa ggctaaagac ccgacagatg ctatttttgg aattaagcct
    57841 gatggcacaa ttgctcattt ttgcatcagc gatacgccac acatgttagt tgccggaaca
    57901 acaggatcag ggaaatcggt attactgaat gaaattcttg ttacagctat ctgtcatgca
    57961 atgcctgatg aattaaagct agggatagta gacccaaaac gagtagagtt tgggcgttat
    58021 aaaaaattac cctatatgct tgctgatcca attactgata tggatgaagc ttatgaattt
    58081 tttgaatacc ttgtgatctt aatgaatgaa cgctacaaat tgatggaaaa agcaggcgtt
    58141 caaaatatca cacaatataa caaatacgct gaaaaacatg gaatagagaa atttccctat
    58201 gtgatcttat tggttgatga atattcgcaa ttagttggaa cacacaaaga agttgaaggg
    58261 ctgatcgttc aattaggaca aatggcaagg gcggcaggaa ttcacgttat tcttgcaaca
    58321 caatcacctc gatctacagt cgtgacaggt attattaaag caaatttccc atctagagtc
    58381 tgcttgatgg tagcaagtga tttagaaagt aggatcgtat tagatgaggg tggtggagaa
    58441 agcctaagtc caaagggaga tatgtttatc aagttgaatg gaggaaacaa aatcagagcg
    58501 caaggagcct atatcagcaa cgaggaaatt gaagcgatct ttgatcacct tagaaatact
    58561 atgccagaac cagaatatgt tgattaccga gcaatcgtag ctgaatctcg tggtgagggc
    58621 ggtgaagacg aggattttgg tggaattggt aaagctcctg taagaaaatc tgctaaaaga
    58681 aatttgaaca cacctttagc aaattcatca tctaaagagc catcaattga aaagaagagt
    58741 acgtttgctc taaaacttga aaaaaacaaa gaagaaaaag aaaaacaagg agctaaaaca
    58801 ctagaaaaac ttcaagccga aaaattagaa aaagttttaa aagcattaga taaagttgat
    58861 cctgaaccgc aaacctcaaa caacacgaat aatgaacagc tgatttcatc taaaactaaa
    58921 ccgaaaacta ctgatccgaa agtttcagta aaagtgacca tcacaaccga taattctgag
    58981 aagaaacaag taaacggaac tacttcacct atcatgcaaa aaatgttaaa agccaaaaaa
    59041 caggtgcata aatgatcatt gtcagttctt ttttgaggtg gcttacgcca tataaatata
    59101 cagatgaagt agttgtgaaa cgagctaaga aaaaagataa aaaagcggtg ttaactccta
    59161 aacagatgct tgatcatgga attttgggtg tgaaggtttt agaagtgaat aaaccgtttg
    59221 aatcaaatag tgcaagagaa aagttgcttg gcgctgaagc agatgtggca tatttttatc
    59281 atgacaaaag caagcatcaa atagctatta aattcaatcc aaaatcggca tggcggtatc
    59341 aagcaagaga tgacttgaaa cgtaggaaac ttagaccaat gctctatcca gaaggaaaag
    59401 aagcatttga tagaacccac atcgttccca tcggttttca tggtacagaa tcagataatc
    59461 gcctattagt aggttggaac agcaatcaaa atagagttca gatcaataaa tttgagaaga
    59521 aagtaagaaa attcaacgaa cgaaaaacaa tcctatggtt tgcagatatt tgtaaacagc
    59581 cggacggaac agcaaagtgg gaagcggtga tatttgacga aaatggtaaa aaagtaatgt
    59641 ctgaaacatg gcatgataaa gctaaatttg tttggcagta gaggtgaaaa ttgtgttaac
    59701 tattctaaaa ctatatagag agggatttaa cgaaaaagta agtattggtg aatccattga
    59761 atatagaggt gataagtata ttattattcg cattcttaaa attaacaaac attattttag
    59821 caaatctatt agtttaacag ttagagttct agttcagaaa gtcggagact tgagtgatta
    59881 ttataactgc aaaagagaag ttattgtgga taatcgatac gaccaatcaa aagatgataa
    59941 gcgaataata aaaataggag atattgtctt tcctgagaaa gggatagcat atgaagtagt
    60001 gtctatcaat agttttcgct atgaatttgt tgatttggtt gtcgaatatt ctgttcgatt
    60061 gatcgttccg tggagtaaag aagaaattaa tgaagcatta aaagatgaac gaaaaagtac
    60121 attcaaagtt ttagaaggtg aaaaaaagtg aaaacagacc ttactagaca agctgagaag
    60181 tgtttatggc atgaaacaaa tcgcatgggt gtatttggtt gttttgaagt aactatcggt
    60241 tgggatggca gagaaagagt agattatata acttattcag tagataacac agttcgatgt
    60301 tatgagatca aagttacttt atccgatttg aaaagcaagg cgaaacagac atttatcggc
    60361 gattataact atttagtcgt tactagtgat ttgtgggaga aaatgcaaca agacgacaca
    60421 ctcatgcgta aatattataa acaaggcata tacgtgttta atgaaaaata tcctagcatg
    60481 ataagtgtta agaaagcaag gaaacaaaaa gtttcgctag gtacaagatc aactgtatta
    60541 gaaagcatgg ttagatcgat gaatcgtgaa gttaagaaat tatataaagt aaaaccattt
    60601 tgggagatag tagagatacc ggaatgatga gtaaggaata ttatatctat tcatatatag
    60661 tattaactga ttatgaggag gatacagcaa atgaaaaatt atgatccgaa tatgcgatgg
    60721 ggtacacata ctcttaaagt gtcttttcaa cggaggataa gctaaatgaa taaaagaaga
    60781 atactgtggc ttattgcagg aatgtatctt tgcatagcag tatctgggtt tactatttgt
    60841 cggataataa atcagttaaa aaacgcgatt gtttatctag gagaaattgg ttatctagaa
    60901 gatttaaagg caggactaca aataatgagt tggattttat tcgttctgac agttgcgtta
    60961 gttatattaa cttgtgaaaa tttttcaaca ataaaaaaac aaataatatc taaccataat
    61021 caagagactg ttaaatcagg tctagaagta gacccagaag aggaagtaac tgaacaagcg
    61081 atcaaagagc aggaagaaat agacaaaaaa gaaataatga gtatttattt atctagtgga
    61141 gagtcatttt ttcatgaaat agattcgctg aaacttagta gtcaagaaat aattgataga
    61201 tggcaaaata tgtctttaga tggggagtta atcgttgagg atagtgaagg ttggtcagct
    61261 tataagaagg acgagattgt agctatcacg attttgtttg aatagtaata tataccacta
    61321 gataatctga atatgttgat atgctagtga cgtaccaaat gtgttcaata gagccgtggt
    61381 cacaggcaga aattgataaa atagtaaaat ttaaccgatt atcaaaattt aacgttttag
    61441 gaaatacctt ttaaaaatca caatacacaa acagaaaaat accaaaaaat taacaatgaa
    61501 cgtaggtaag aacatgacaa taaatgataa taacctcaaa aaatataatg ctcttgtcca
    61561 acgctatatc gaaaacaaaa taacgatgca agaggtcgct gatgaattag gcaaatcaaa
    61621 aagcctagta tcacacgact ttaagcacaa tgtttttccg aacccgattg agctgaggaa
    61681 aaaatttcaa aagagagttt atcaagaaat agggaagtgg attgatgaag ggaatgcaat
    61741 tgacccttta attgaaaaat atcctttttt aaaaagcaaa aataaaattc aaaggttaat
    61801 tcgtcaaggc gatattcaag ctaaagaacc tgttttgaca accaatgatt tggctaaaat
    61861 aaatcttcat aaacaaatta ttgaagactt tgaaaaaaat ctttcaacaa ctgaaatttt
    61921 aaaaaaatac aatattccga gaggaaagct aacgtcgact ctttttagag aatatggaga
    61981 atcggcagtt actaaagcaa aggaagtagc gaatgagaga aaaaaacaaa aatagtagtc
    62041 gatacttagt agcgactgta gtttttgttt gtgtattgat tttcctgttt gattttttac
    62101 agattggaaa aagtataatt ggaaatacat tgagtgcaaa ggaaatggtt gctttaggtg
    62161 gtttgtatga aggacatttg ttcccgaata tattcatttc tatcgtttct catattaact
    62221 tgtaccattt aatttctaat ttattgttct tagtagttct tgaagaagca ctgaatgatc
    62281 agtatgagtg gttaacatta ttgatcgttt taatcatatc aggatttcta ggaagcttag
    62341 ttccttttct gctacaaagc aacatcgtaa gtatggggct tagtggaggt atttttggat
    62401 ggattgcttt atttgcgatt gcatacatcc attttgatag caaggaacat ttattactaa
    62461 gtgtttttgt attattgata atcggcattt tattggtatc tacttttgaa tcaggcgagg
    62521 caaatgtctt gtcacatgta atcggatttg tctgtggaat gatcttggga tttattctaa
    62581 agaacgtttc attgatcaaa caaaagaatt aaaagggagt gctaaaaatg ccaaaaagaa
    62641 aagaaaagaa aattccagtg tttttgtctg tctcagaaat gttatcactt caaatactgt
    62701 taagaaaaaa gattgaaaca atagacgaag aagttagaaa taaaagggct atgtacgaac
    62761 aacgtgaagc gctaagagaa cgaatccact attttgatga tatacacact aaaatctatg
    62821 aagcacgtac aaatcacgaa gacgaaagta aattaggatt agctcaacaa cggatacttg
    62881 aatggcttaa tacgaatact gatcaagaac cgttaagagt gattagtaat ctttttgaat
    62941 ttggaacgaa gcattggttt aatgctgtag agaaggatgc tgacttacaa gaagcgtttg
    63001 atacgctaag taaggatagc aaatccatgc gagatttatt gattacttat ctaaatgagc
    63061 atgagtttga acaaacgaaa accaaaaaga aaaataaaga atagaaagtt gtgatgaatc
    63121 atgctattaa ccctgttagg ttatttatgt ttaattcttg ctttttttgg ggcaatcgct
    63181 ataattattg gtcaatttca aacaaataat ccgattatgc gtaacatagg cattggaatt
    63241 gtgattgtaa cctttattgt gtttaatgcc actaagattt ttggaagtaa cgataaacaa
    63301 tttggtatat cgggagttaa taatagttat gctccagatt ctttgggaag ggtagtaata
    63361 gatggaacat ttgaaaatgt aacgagttat aaaaatgttt cgctgacagt gaaggaagat
    63421 ggcaagaaag catcagacga cgttctcgct actattaagc cgtctcaatc acaatttgaa
    63481 gtgttttata cagttaaaga gaatagccct gtacacaagc tgacattggt ttttaacgtt
    63541 gatggagaag aatactataa agatgtaaaa ataaataaaa catcaacgaa aaagattatc
    63601 gaatcagcga aaaaagaagt agctacagaa gaaagtacaa ctgaatcaag ttctactgtc
    63661 gaagcggaac taccagataa aattgatttt gatcctgaaa aagtcaaatc gtttgaatct
    63721 gtttctggtt ctatggctga tcggtttaac actgaagtct tcaaagctat caaaactgtt
    63781 aaattggata ctgaaaagca aatgattaat gttaccttat tcaattcagt tgaatctatg
    63841 gatcaggata aaaaagttac ggttgcaaga gaactaggaa aaagagtaat tggtttgtat
    63901 cgtggtactg ctaaaccaga ttcagacggt gcagatttgc cagttcatgt agtttatgaa
    63961 gacggtaaag tttttggaga ttctacagca aacgaagcat taacgattaa attggctaaa
    64021 tagccagaaa gttataataa acaaatggtg tcacaccaat aatatatagg tgtgacacct
    64081 tggagatgat agctgatggt agtaaataaa gaaaaagtta gaaagtatgt gactttaaaa
    64141 atagcttctg ttaaatattt agaaaagcga gctaaagaag aagaaagagc aagaggtaag
    64201 aaagtaacag tttcagagtt gttagaagaa ttgatcgaga atgatcaaac aataagacgt
    64261 gcatttacta ataggtctat cagataacaa tataattaat aaaaaagaaa gagtgatttg
    64321 gtcttagact aaatcactct ttttatataa agtttcttta cactcattcc gaataatgtt
    64381 aaaattcttt atatgatata tagttccttg tcagaggaag agataaccag caatattgga
    64441 gggataataa gtggaaaaca ataatgatat tagacatttt agcattgggg aaagagttac
    64501 aacagtaagt gaattatcaa aggattatag agataaagag ggaacgatta tttctcttaa
    64561 taagaatcga agaggcgttt ggcaatatgt tgttgattta ggtgatggcg ttacagttga
    64621 gtgtaaagat agtcaactga tcagaaataa tggcagtcat tccattgaaa aagcaattct
    64681 tgtataccca aaagtatcaa gtaagatcat gaatgactgg ttgcttggaa aatttggtga
    64741 gtacaataaa aaagaaatcg cagaagtgat cggcaaatct tatccaacag tattagggta
    64801 tctaaaaaag ataacgagta aagaagcaaa aattgaggat gttttaacag taagagagct
    64861 tcaatcatta atcgagtatc ggaataaaca aataacaaat cctgagtacc ttagaaaaga
    64921 agatggtcta ttgaaacaat tggaacaagt aaataaaatc cggaagatgc aaaaggtagc
    64981 tcctttaagc cttgatacct ttaaaaaaat ctatttgaaa gagatcaaaa aagactaatg
    65041 tggactgtaa ctttaccgtt ccaacctgta tctgcaccaa gaccaagtgc aagaaggaat
    65101 gctgctgatg aaactaatga cgtagcagag aaaaggattt caacctacta tgataaaaag
    65161 tatcttgatt atttgaaaaa aataaagagc tttatcgaag atcaagattt attagatgat
    65221 gaattttact cgattgtaaa tgatcctatg ggcgcaatta tggaagtgga cttttactac
    65281 cagataccaa aaagctataa aaaagtctat aacattatga agacaacagc gccagattta
    65341 gataatttag taaaaagtgc tatggatggt atttttaaca tctcagctat acgagatagc
    65401 catatcacag gcttaattgc ttttaaatat aatgtggcga acgaaggaaa aacagttgtg
    65461 aggatcaaac gatattcaga atttgagtgg gatacttctg aaggattgaa cggagatata
    65521 tgggaagcta catttgattt taaaccagta gcaacgccaa gaccaaagtc taaatttaga
    65581 ggaaatggga ttatcactta ctatccaaaa gagtacaacg actatttgga aaatatcaag
    65641 aatacgctta aagagaaaga tttggtaaat gatcaactga aaaaggtaat gaatgctcct
    65701 atgggggtta tagctcagat agattatttt taccaagtga gaaaagataa gaaaaagctt
    65761 gattcactga tgcgtacttc acagccggat attgataatt tagtcaaagg aacattggac
    65821 ggaattttta acaaggaaat aggcatcaaa gatagcaggg ttgtagggtt aattgctttt
    65881 aaatacaata cttttgaaaa gtcgaaaaca aacgtgagac tacaggagat gggctgatga
    65941 ataataatga aaaaattctt catgtacttg atagctttga aataattcaa gaagaactaa
    66001 aaaaatatcg ggatgttttg gaacaaagat atgattttgt caatcagcaa aagagtaatc
    66061 acatggattt cattctcaat atgaatgact tgaagaagaa attagttgaa cgaaaagaac
    66121 aagaaaagtt gatcaaagct tactttgaac tcggtgaaaa agaagttaaa aatgcaatgg
    66181 aactgaatga agagcgcagg gttttagatc agctgttaga acaattattg gtgatgtttc
    66241 aaaaaggacg aatagatgag gatttaattg aagaaggatt gagaaaatat ccttcaaact
    66301 caggtatcgg aatagtgctg aaagcgattg atgaagaaga aattgaagct ttcataccgc
    66361 cagaggattt tgaatcggca atggaatata taaaatatta tagccaaggg atcactgctt
    66421 ttagagagtt tgatcctgaa gatgttattc atgacttgaa caacctaaaa gaatggtgtg
    66481 aaagttatgg agtcgatgat tccggattgg actacttgat cagtattatg gaaatcgaag
    66541 aagagatgcc ggataagcct gatccaacag atattttaga attgatccat gaagctagaa
    66601 atccaattgc ctatatttct agaggatata ctgtattgga atactataag ccttatatat
    66661 cagcgatgaa ccatttaaga cgtgtattaa gggaaaaacg tgaataccgc tcagtgttga
    66721 atgcaacgaa tagattagaa aaagctgttt cagagctaga tgcttattat cgtgaacatt
    66781 acctgcaaac aggtggtatg ccaagaaata cgaaagcaaa tatcagtagg tatataaaaa
    66841 aagctgagta ggcggagtta gcccataaaa tggcaaaagg taacctgaaa ggggcatttt
    66901 gaatgaaaag ggatgttttt gaattgtacg tattagaacg tctagaagac ggcatgatgg
    66961 cacaagagat aattgatgaa attgatgata tgatcagcag atttggtcaa ttggagttga
    67021 atgatggaaa caaaaattcg gtagtgctaa aacaattttg gttgaacaat ttttcgtata
    67081 aggattttat caaagaaaag gcaaatacat tgtactgact aaattcacga ggagcagtga
    67141 tgagagctta ttgctaagag cagttaggct cttttttgtt tgtgtaaaat tatttgtttt
    67201 ataatacaca tttatacaca aaaaaatatt gacagatagt acacaaatgt ggtaggatat
    67261 atttgtaagc aagttagatt aacaagttaa aggaagagca gttatgaaaa aagaagaact
    67321 aatagatata tttcaaattg ttgaacgagc aaataatatg ggaattatgt tttttgatcg
    67381 tatttcttta aagatggatt taagcgttgc ccaccaagaa tttaatctgc gattaaaagc
    67441 cttgttgaat tctgatgatg ttaatttcgc tcatgatgtt gtaggtatac agaaccatat
    67501 agatagagaa aacaaacgta tgggtgatga attcttacca agatattcaa gtttatgatt
    67561 tggcgaaaga ttcaagagta tgtgtcatta ttgataggta ctcttgaatt ggaaaggagg
    67621 aatgaattta agacaacaac gattatgatc ttaaaacaat cgtttatgta attggcaatc
    67681 ttacgaataa ttttcatgca ataatgacaa taaaaaatga aaaggggtaa aatcaaatga
    67741 aaaaattttt agtaggaatt ctattcacaa tggggctagt attattatca ccttcagttt
    67801 cagcagaaag ctctatttcc atgtatcgta tgtataatcc caatagcgga gagcatctat
    67861 acactagaag cgttggagaa agagacaatc tgaaacgtta tggatggcat tacgaaggtg
    67921 tcgcatggca agctccaaca agtggtactc ctgtttatcg tttgtataat cggtataacg
    67981 gtgaacattt ttacacagtt gatgccggag aaagagataa gatttcacac acaggatgga
    68041 cgtacgaagg tgttgcgttc tattcctaca acggagcaaa tagagttcct gtaacacgtt
    68101 tatataacaa atacgtgaat tggcatcact acacgttaga tcaaaatgaa aagaacgtaa
    68161 ttagtaaaca aggttggaag attgaaggta caggctggta tgcaatgcca aacagcgtac
    68221 caaatcctac acctgctcca agcactgaaa aagtgttgaa cgttcctgtt gtatggcaag
    68281 gaaatacaat gttatgcgaa ggagcttctt tattagaagg tttacactac aaaggtgtag
    68341 ccacaaatca aagcttaacc agttttgtaa actatatgcc aaaagctaat gacaataatc
    68401 cttatcatgg ttactcagga gagtggcgtc ataacgttaa tggaacgtat caaggcatga
    68461 tggctgaccc agtagttaaa tgggcaaaaa acgttggcgg taatgccgtc aatatcactg
    68521 gttcaggaac gaatggaatc aagaatgaaa ttcgcaatgg taatccggtt gtaacttgga
    68581 ttacttataa ctatgctacg ccagagttca aacaaatgcc ttggggtcgt gcagtatgga
    68641 atggacatgt agttctcgtg gatggcttca gaaatggtgc ataccatgtt gttgaccctg
    68701 tatttggcgc aaaatggatc aatgcaagca cgtatgagag agcatttaat gtgactggaa
    68761 tggcggttgc tgtacgttaa ttgatagaat tatcaactaa atgaaataag agcttaatta
    68821 ctgaaatggt aaataggctc ttattttttt agaggaaatt tttgtaatac gaatctataa
    68881 caaaaaatat tgaatgtagt acacaaaagt ggtatattaa aatcgtagag attatgcgaa
    68941 gaggtgattt tgatgaacca aagaagaaga aaaaggttag aagaaataag cgagcgactt
    69001 tccgagctaa aggatgagtt agaagtaatt caagaagaag agtacaaaga aaatatacct
    69061 aaaaatctac agggatcaga aagatatgaa aaagcagaag aatccgttga tcaacttgaa
    69121 gaagctgttg atggtttatc cgacatatta gaattgattg aagatatagc tagctagaga
    69181 tgattaatga agatttcagg agtgagaata aaatgattga tatttcactg atagagaagt
    69241 tacttttctc aaataagtac accgcaaaag agctggctac aagatggcaa atatccgaat
    69301 cagctattaa agcctatcgt caaaataagg gaagcgtagg atggcgtgat tggaaaggga
    69361 tcagcattgg aaaagcagaa gacataatga aacgtgaagg acttcgatcc tataccactt
    69421 ctgaaaaaga caaataactt aatgtttgaa aagataaaat atctgtctta aagctatgtc
    69481 aaattcaaat ggtgaatgtt tgctaaatat aatgcaaaaa aaggaaggga aatattaaat
    69541 gataagcgaa aatttgtctt atgagaataa taaacatcta gttaaagaaa ataagaaagt
    69601 agaagattat aagaacatgt atatagaaat gaaaaaatca ccactgtgta ttcaatataa
    69661 aaatgataat tggaagcaag tagtcgaatt tgtaaaacct catagtatac gaagagtacg
    69721 aagggggaag aaaaaaagtg aacttgtaaa tagcggaaga gcggtaatta gagttataac
    69781 caaagattat ccagttgaat atggagatta tattttaagg ttaagttatc atgcttttgt
    69841 tgtgttgaaa gaagagcatt tcaacgagtt tttcaattta aaaagtaagt aatcatattt
    69901 ttttgtagac gtgtataaag ttaactaaat tgcgaggtga tgacatggta gcaaaaaaga
    69961 atactctaaa agaagcttct gaaaaatatc aagatatgaa aagacgagga ttaataccga
    70021 aagacaaaaa aggtatatgg agagaatttg ttaaggaagt cagtagtgat cgaagtggcg
    70081 gtgactttgt tggacgatat acagcaaaag gctatagcaa acgattaagg atcactgttg
    70141 aatatggaac gaatgaatca gctacagagc tgaagcaaag gtacttagat acacgaaggg
    70201 aagcttcaaa aaaatataat gatttcaaga gcagaggtct gattcctaaa aagaaaaagg
    70261 gaatttggcg tgagtttttc aaggaagaag atagcaatcg gaataaggga gacttcattg
    70321 gacgatacac agcaaaaggc tacactaaga atttagggat caaggttgaa tacggaactg
    70381 gtgaaagttc atgggaattg agaaatcgct atttaaatac attgttagag aaatataaag
    70441 cagaattatt ggagttgttc gatgttaatg attttatcga gatggctgaa aagtatggtc
    70501 atgaagaagg ttttatgatc aatgacatta tggttattga taattttacg gatttattag
    70561 cacttgcgaa aaaagcaagg gatttaagag aaacgcagat gtatctattc agaaataaaa
    70621 acggaagaaa ccggagaact gaatcgagtg agattgaaga ttactacgat gcctttacac
    70681 acgatctggg agcctttgta gacgagataa gtggtgtaat gaacaaatat agggtctaga
    70741 ataaaaaagc cggagatgag cagataaatg ggaaacaata gcatatacac acctgaaatg
    70801 cagaataaaa ttaaaagact tgtcggatta gggtggatga caagaggaaa attagattct
    70861 tttagtacga tcaaatatct tttgatggac atttaccgtc agaacaagca aacaggaata
    70921 cattttttgg gctatgataa gccggaagat gcacctttct ttgttccaag ttcagcctat
    70981 agagccagta cctccggtag catgtataac ttggatttgt ttgaggaagg agcaaagatg
    71041 atcgaatatg atttcaacga agcctattcg aggatcatga gaaattatcc tttgccgtca
    71101 aacacttatt tgaaagcaaa atataataca gatcaagcta ttttgagagc gataaataga
    71161 ccaaaagtaa gaccgtatga aaatttaaga acgtatatgt tctttttggt taattttgaa
    71221 ggaacagcca aagaaggaac atatgctaat ccaaaatcac agtcgatgtt tgcggattat
    71281 ggcgaaaatc ccaaaacagc taatgagcct atttggttat cggagattga gctaacactt
    71341 atccttgatt attatgatat taaagagatg gaagtcctag atagccacac atttttgacg
    71401 aaaaaaggat tactcaaaga atattttgaa agaatcgagc cattaaaaac tgatcctgat
    71461 acgttgaagc tttataaaaa tcttagaacg aaagtatttg gtgctatcgg acaaaaatca
    71521 ttaagagaag gagaaacaag caccttcgat aaatacagta tgtataatcg agcgtattcc
    71581 tccatggtag caggcgtatt tagagatcgt atggttagat atgaacagaa atacgttcat
    71641 agcgcatatg ggctattaaa aattcggact gatggattgt atttcagatc cgaagtacca
    71701 gagtttgaag agctgtataa acttggagta gttaagaaaa aagttcatac aatatccaaa
    71761 gaagaagtcg aaaaagcttt agaatttaaa aacaaaggat ttgaaaataa gggctccttt
    71821 gggaactcta aagatgaagt taagggtgtt gtgatagacg atgatttcga gtttgatttt
    71881 gactaaatac tctttggaaa agatggtagg aggtaaatat gaagaagaaa attgtggaag
    71941 gaaatgtagg tgttattatt ctaaaagatt caactgacta tgctaaatca atggcactaa
    72001 aagacgaaaa agttgcacgc atggataaag atttagttga atttctgatg gatgatacag
    72061 aaagattaaa aggggatcag tttttgagtt taatgaaaga caaaggctta aacacatatg
    72121 gtttatttga ttataagaaa ttagctatag agtgggtaaa agaaggagaa atatttttta
    72181 ttgaagaatg ggaaggtact gagaacttag ttcttcatca gtatcttttg aaagcttaat
    72241 ctaagtaaaa cagttctgac tagttatgct tgtcagaatt tcaaaggatg ttttccataa
    72301 ccatcatcat tcaaaaataa aggatttgaa aataagggtt cctttgggaa atctaaagat
    72361 gaagttaagg gtgttgtgat agacgatgat ttcaagtttg attttgacta gatttttttg
    72421 aaaggtttaa gagtaaacaa ggtaagaact aagatcatga agattgagag taaagaagat
    72481 agaggacgaa tgtatgagaa cacactatat ttatttagaa gataaaattg tagatgaaat
    72541 tccggcaaaa agtcgagaac aagctagaaa attatttatt gaaaaataca aagagatcaa
    72601 tggagatagt gaagttccaa aagtggtcgt aacagttaga aaaaggcaaa caaaaaagag
    72661 aagtacgttt catgtcagcc ctgatatata taatcttata gagcgacata gaagtgaaga
    72721 taatatttct atctctcaat atattgctac agttattgat gaattgattt caggaaagta
    72781 tgactatgct tttgattttg aggatgaaaa ggattacaaa agtgttgctg tctttcaatc
    72841 tgattttgag aagctaaaaa actatgctgg caggagaaat gttaattttg caagactact
    72901 agaagagttc tactccgaag agttgggagt aggaaatgaa tacaagagag aaacttatgc
    72961 gaaattgtct cataagttat cccaagcgct taaagaagtg acagaagtta aagcggaact
    73021 agatgctaaa cttgaagaag tgaaaaaata tcaaacagag aggaagaaca acatgaagaa
    73081 tcaagtggaa tttgacgaac agaaaaattt gaaaaagcaa gaaaacgcta taacaaaatt
    73141 atgaatgaac ttagctatga acacttaaca gttcatgaag aattgagtga gggtacagaa
    73201 aaatatacga tcgcagatat ggttaatgaa gtggaaactc ttttagcttc tttctttgaa
    73261 aatggtcacg taaacgatga atggcgaaaa gaagaaccta aaagggttaa cgcttatatt
    73321 gctcgattaa aaaggtttat tgataaatat aaggagaata gtgacaaatt ttctaaagat
    73381 aaataaggag agagtgtgat tacatttgcc agaaatcgaa ggaatatttt cgccaaaaat
    73441 aggtaaaaaa cagttagatt tcagcagttt tccgagcgta aatagagatt ataatctagg
    73501 tgatgaggta cagatactaa atattgattt caaagaacta gacttcgctg ttgctgtttc
    73561 caacaaaccc aaggcattgg aacgagtgaa aaaaatatac caagagaatg atcaaaatat
    73621 caaaaatatg atcaaaaagt atagacgaga taattcaaaa gttgaagacg ttttattcaa
    73681 cggtacggca aaacatgatt tgaatgttta tatggcatta ggagtggctt tgtactgtaa
    73741 ttcaattaat cagacagata ttttggttaa tatcgtgagg aatagctatc aaaaaatatt
    73801 aaaaataagt agcgaaacaa acccttatta tgcaatgaaa tatcttgggg ataatttatt
    73861 aaatgataaa aaattgatgc acaacactga agagctgtta tctaatttgg ctgcgattaa
    73921 ctttctattc aaaacaggtt ctgaagatta ttttatagat aacgtactaa aaggtgctta
    73981 cggaacagca ttttatccgc tttacaatat gaaacagcat aaagccgaac aaaaggataa
    74041 caaacttgaa attaaaaaga tgaaagaact attaggtata ggatttgtaa agcccaattc
    74101 aaaagtcact attgcttcgt tagcaactac taacaatgat gggaaaatag taaatgggtt
    74161 tgacgattca gatatagata tattgtcaaa atcatatttt tctttgtatg gagattttgc
    74221 agacttgatg ggtattgata gttacttagt caataatttt gagatcaagg gtagcgagct
    74281 gaatgatatt gtgtatttcg ttttaatctc tctatatgca attgatcaga ataccgagta
    74341 ccgacaaatt gactatttgc tagtttttta tagtttgtta tatcagttcg ttttaactcg
    74401 gttgtttaaa aatgctaagg aagaactgat cctgaattct gattatgaaa tagcaaaaca
    74461 aattgcaagt attagaaaag aaaacaaatc tgaagtaatc aaattaagaa aagaattaag
    74521 tattgcgagt aaagaaaacg gtcggttaga atctttagtc aaatcggcaa aaaaagaaga
    74581 ggaacttttg aagcaagaga ttaaagaatt aaaaggaatc agaaaagaat ttgaacagct
    74641 gaggtttgaa aataaagtat taaagcaatt gcaggaacaa gaacatagac aatcattaaa
    74701 tggtaaaact gaagagcctt cactgtctga aatgatagct tatctaaaac ataaaaacat
    74761 tcttttagtg ggaggacatg taaattttca aaataaatta actgaactat taccaaatat
    74821 taaaatgttt gatgtaagcg aaaataatgg aaagatactt aaaaatttga atacgtgtga
    74881 tgcgattttt ctatatacag attacttgaa tcatggtcta tattatcgtg ttatgagtga
    74941 aatttctaat aaagataaga tattttattt ggataatcat gtgaatatga acttaacaat
    75001 taaacgtatt tacgatcttt tatcacttat ataaagagag aagtgataaa taggacacaa
    75061 aatttggaaa gtaggtaagt cactatgaga gaactaactt tatcttctga ggaattagaa
    75121 aagaaaactg ctttattaaa cgaaatattt agtgctgatt gtataatcca aagtttaaaa
    75181 gaaagttcat tttcaaaact aacagatcag aaaaaaattt cttatatgct aagtttagat
    75241 aatctaggaa ctaaatatga tgaatcggca agtagcgata agcttttgat tgaattagta
    75301 tcaagtctta gtagttttgg aaaaatgaaa agatttaatg tgatattaga aatttttgct
    75361 tggataatcc cttcatttat ttggtttttt tatattttca tagttgaatc tgagttaata
    75421 gccaaggtct ctcttgaact gttaattccg atatatgcat tttatttaat aggttcagca
    75481 atttacttta ataacttgta tacacgtatt caaataaaaa agcagttatt acatgaagga
    75541 ttaagtggta atatgttaaa aagaattgca tgggataaag ataataaaac agttattgag
    75601 cttaaagaat ataaatacaa ttatttctcg agatgtatag atagaaagtt actgtttaaa
    75661 aaagaaggat tagaatttag ttttgatgaa gatgaatttg aaaaattagt gatggactgg
    75721 cgtaagaatt tactggatac ggtacaattc taaaagcaat tgaggtcgaa attgcgatac
    75781 ttgaatcatg gtctatatta tcgtgttatg agtgaaattt ctaataaaga taagatattt
    75841 catttggaca gtcatgtgaa tatgagctta acaattaaac gtatttatga tcttttatat
    75901 gtttataaac attaatgagg tgctaaaaat gacaacaata actatgacaa aaaatataga
    75961 agtatatgtt gatggatcac aagaagaaag ggattccttt tttgattttt tttggaaaaa
    76021 tagtagagaa tatgtaaaag cgttgaatct ggtatataaa cagctaatgt ttgaagagtt
    76081 gataaaagat gcaattaaag aaatggatca aggttatgct gagagagaag aaagtattca
    76141 aaatcggatt gaaaagttag catatactga tccaatagat caaaaaaaac ttgataaagc
    76201 aaaagctgaa ttatctaaat tgagaaatga aaaaaataaa gaagcgagag aaatattaac
    76261 gcaagcgata gggttgcaga gtcaaacgag aacacgagat gtagcaaaaa aattagactt
    76321 tgactttgga gattttgttg attcagcaaa tatgaaagct agtaaggatt ttggcaatga
    76381 ttttattgga attgttactg gtcaaagaac acctagaatc tatagaaata aaagtaaata
    76441 cggattgtta ccgttcagag gtcgggatat taagattcac aaagctgaca agttttatat
    76501 ctctttacct aaaggattta gacttaaagt aaatttaggt agcaagccaa gaaaagcaag
    76561 agatgttgct tcagttttac aacacattat ggatggcgat tacaaattaa atcaaagtaa
    76621 tcttattttt gatggaagaa aattattttt taatctaaca atttcttttg aaaaaagcta
    76681 taatgaaatg gaattcaaga aaggtaatga attgatcgta aactttgcag atttgtgtag
    76741 cggtgaatgt ttaattaatg ataaatctgt aatgtctttt ggtgatagcc aatatataag
    76801 aaacttcaaa agtaaaattt tagcaatgaa atcaaaagaa caaagccgag gagtcttttc
    76861 taagggcggt catggtcgaa aaagaaaatt agatcaagat cgatgggaag caataaagaa
    76921 aagagaaagt aactttgtta aaacttataa taatcaatgt tctgcaaaaa tcgttaaaac
    76981 tgctattcgc aataaagctc aaattattcg tatcattgaa ccagataagg cttatggcga
    77041 atgggcttac tatcaattga aagaacaact agtaaacaaa ggaaaaagat atgggataga
    77101 agtcagaagt tagtaaatta aacaatcgtt gatttggcaa cgaaatagtg agggaaaatc
    77161 cactcactga agggtaaact ccgtcaagtt gtgtgaccag acggaaggac gaatgttgtt
    77221 gaggttttag aactatataa aacagcatgt ttctcaaaca aagtgcagaa cgaaaatcaa
    77281 ttcatcacag ttttaggacc atataaaaca gcatggtttt aaaacaagaa ataagtcagt
    77341 tttccttttg ctttagtaag gaaaaataga gtattctcaa taaaacttaa aataagaaag
    77401 aataagcaaa cctttgtttt aatagctaat cgaagtaaaa cccaaagcca tatgaactcc
    77461 tgaatcagcg aaagtttatg atgattgggc ttactaataa tttaggaatt agatggtaaa
    77521 tctaaattaa acaatcgttg atttggcaac gaaatagtga gggaaaatcc actcactgaa
    77581 gggtaaactc cgtcaagttg tgtgaccaga cggaaggacg aatgttgttg aggtttagaa
    77641 ccacataaaa cagcatgttt ctcaaattgc cttagtggtt gaattgagat ctatcacgtt
    77701 ttagaaccat ataaaacaac atagttctca aattgtttaa aatgattgtc aagttaaaaa
    77761 aatctgggat catataaaat agtatagttc tcaaatatga ttagaaaccc tattaaagag
    77821 caatattaaa ccaacttact taaatcgttt aattaagaac agtaatctct tttagtcaga
    77881 cttttagtct gacttttttg tatttccaaa atttattctt tacaaaagcg aacgtacgtt
    77941 tgtataattg cttgtgaatg ggggaataaa aatgtttgat tacagtagag aaccggtgaa
    78001 tgactatttt ttaatcgaca tgaagtcttt ctatgctagc gtggaatgta ttgaacgaaa
    78061 tttagaccca atgactacac cgttaattgt catgagtaga tcggataaca ctggttcagg
    78121 tctgactcta gcttcttcac ctactgcaaa gaaaaaatat catatcacta atgttagtag
    78181 agctaatgat ttaccttatc cattaccaaa agaactttat attgttccac ctcgaatgaa
    78241 cttatatata aaaaaaaata tggaagttaa tcagattttt agaaagtatg tcgcagatga
    78301 agatttattg atttattcga ttgatgaatc aattttaaaa gtgacaaaat ccttaaattt
    78361 atttactagt tcagaaacaa gaagcgagag gagaagagaa ttagctaaaa taatcaaaga
    78421 tgagattgag aaagaaacag gactaatagc aacagtcgga ataggagaca acccattatt
    78481 agctaaactc gcactggaca atgaagcgaa gagtagtaaa gaatttatag ctgagtggac
    78541 ttatgagact gtacctgaaa aaatatggaa aataaacgag atgactgact tttggggcat
    78601 tggatcacgc atgaaaaaaa gattgaatca tatgggaatt atgagtatag aagatttagc
    78661 aaattggaat ccctacacaa ttaaaagtca gttaggtgta atcggacttc agttatattt
    78721 ccatgccaat ggaattgatc gtacggatat tgctattcca ccagaaccaa ccaaagaaaa
    78781 gtcttatggg aattcacaag ttctaccaaa agattatacg aggagagaag aagtagaact
    78841 agtagtcaag gagatggcag agcaagtagc cattcgaatt agacaacata attgcaaaac
    78901 tggttgtgta cgcttagcaa ttggtacgtc aattttggaa gaaaaatcag gtttctccca
    78961 ccagatgaaa ataccaatta cagataatac gaaagaatta caaaattatt gtcttttttt
    79021 attcaataag ttttatgagg ggcaggaagt aagacatata ggagtaactt atagcagatt
    79081 agtatacaca gattccctac aattagattt atttagtgat ccaagaaaaa agattgctga
    79141 agaaaatttg gataggatta ttgataagat tagacaaaaa tatggattta cagctattat
    79201 acatgcttca tctatgttag aaggtgcaag aagtattacg agaagcactt taataggagg
    79261 acacgcagga ggattaggag gtttgcataa tgattgaata taacgataga ggacgtttaa
    79321 aatggtcggg attttatttg tctgatcata ctgaaagaat caatgcagac aatgtacaac
    79381 gtgctaatca taatttggca aaagaacaaa tgacgactga agagattgcg aaaatactga
    79441 gaactgctgt cataaaaaat agacccgtct gcattcaaaa agaggaacga aacgctgaag
    79501 gttactatcc agatgatgta gtaggaaaaa ttattggata tgacgggctt ggtgtatttg
    79561 tggaagaaga aaaagttcac tatgatgaaa ttaggaatgt ttctttcgca gaattattaa
    79621 aatggagtca gatatgaaca ttattaaaaa atacgaattc ggttatatta cgtatgaaca
    79681 gctaatagag gaattatggg gatatggtca acaattaatt aatgaagtgg ggattgattg
    79741 cttttgtttc tatattgaat ccggagcagg ataccataag aacagatact atatatttcc
    79801 atatccacca aaatagtctc ttagagattt ttaaattttc tccaaatact ttcaattatt
    79861 gtgaaatatt tccctttaat taatcaaatt ttttataaaa gtaatatata aaataaattg
    79921 cttgaagtat tatatttaga tatcataatt tatggaagaa aaattaatta aaaaggatga
    79981 gattataatg gggttactgg ttccgagagc taatacttat actatacctt taacgaaacg
    80041 ccaattaatt gctaaaagat atcaaaggat tactagagcc atcaatagag agttttggaa
    80101 ttctgaaagt gatactgctc acagtttata cgtaggttct tatgggagag gaacggcaat
    80161 tagtactagt gatatagata taatagttga attaccgatg gcagaatttg acagattcaa
    80221 aaattattta tcaaatggcc cgtctaagtt attacaagtt ataaaaaacg catttcagga
    80281 aatactacca aatagtgata ttagagctga tggacaagtt gttaaaataa atttccatga
    80341 tggaatcaaa tttgaaatag ttccagcttt taatgaaaaa gattattggg gtgagtcaaa
    80401 agggtttatt tatccagatt caaacatggg agggaactgg aaagctacaa atcccaaaaa
    80461 agagcaagaa gccatgaaat taaagaatac aaaaagtaat aatttattat atgctacgtg
    80521 taaacatttt cgacatgtac gagatacaga atttacgagt tatcatcttt caggaatagt
    80581 aattgacagt ttcgtatatg aggctatggg aaattggaaa tttgttgaaa ataacagtgg
    80641 gggtcaaaac attagttctg tatcttatga gacagcttta ttagaatact ataactcaca
    80701 taaagttatg ggcggtttaa atttatactc tcctggaagc aatcaatttg ttaattctga
    80761 tagtagtatc atatgtttag aaaaagtatt aaaaaaaata gctttataaa gttttatgga
    80821 ggaacaacat gggagacgaa aaaaagcgct tggaagttca attacgtgaa gcatatggac
    80881 gagtaacgta tacttacacc actcatttaa agcaagccga taatattatt tctgacaata
    80941 aaattttaag gtgtcttcaa attggtttgt cagcccttac tactggaggg atacttagta
    81001 ccattattac tgataatagt gagttaacct ttgttgctgg attatgttca acaatttctc
    81061 ttggattaaa cttatacttt aagaactttg atcttgctga agaagctaaa aatcatcaaa
    81121 ttgttgccga taaattatgg ttgatcagag aagaatacgt aagcttacta acagatttaa
    81181 atcgtttaag tgttgaagat attgtgaaaa aaagagatca attaatgaac gaaactcatg
    81241 aagcatataa aaatgcccct aaaacaaata gtaaaagcta tgaatcagct cagaaagcat
    81301 taaaatctga agaagagcaa ttttttacag aagaggaatt ggataagatg ttaccagagc
    81361 atcttagaaa aaagagttct aaatattgaa ctcttttttt atgcttaaaa ttctcttgta
    81421 atttttctat tcaatcaata aataatctat atttaagaag aaatatgatg atagaaacat
    81481 ctcctaaagc caccgcaagc cgttctaagc atagcttagc ccgacattgc tagatatgct
    81541 catctcacca tgaacgtttc ctaaagccac cgcaagccgt cctagtcatt acctagtccg
    81601 acatcgctag attagtgcat ctcgccataa aagttttcta aagccaccgc aaaccgtcct
    81661 agccatcgcc tagtccgatt ttgcttgatc ggttccattt ctgtttattt agtgggtcaa
    81721 caccgtaatc ttaaatagcc atcgcctatt taagattact aggtctgctt tgaaggtaaa
    81781 aagtctttca ttcttcaaga cttttaggct ttcaagctac gcctgaaagc attgaaattc
    81841 atcgctcttt atttggtttt gccatttata attccttacg cccattatct ttttcgcctg
    81901 tcttgccttg tcagaagtgt ttagagctta tctcatcttt ggattaatac aagtggcgta
    81961 aatttttttc taaacgcaag cgttacgaaa ataatttact taaacacttg tattaatccg
    82021 gcagataaga acgcattcta atcactcccg acaaggcaag acaggcgaaa aagataaata
    82081 aatgaacgaa aggaagaaac ataaaatgca aaaccaaata gctatgaaga aaaaaacacc
    82141 tccctctaaa acaatccaaa caaaaaagaa ttttgaaaaa ccaaaaatca aaaacagaaa
    82201 gaagcgttca acatgagaga tagaatttta gaagttaatc aagaaatggt aaacaagtta
    82261 acaaatatca gtgggaacta tgaaaagtac gatggtactt tacttgataa ctatattttt
    82321 cacgatacac aaaatatcat aattggcaga agtaaaccga gagcgttcat tctcatcaga
    82381 gaaaaatatt tgaatgaatg gaatagcacc tatgaaatgg ttatgactga taactttgaa
    82441 aaggtattgg atttcagaaa tgctttgttg ggttctgata tgggaaatag cagtcttgaa
    82501 tctattgaag ttgaaaacaa gaccgagaat gaaggtgaag gattgattaa gaatgtcttg
    82561 aaaatcgaac aaaaatatat cagtgaagat agaagttttt atagagtcac aagctcggtt
    82621 ggtctagcaa actttgctac taacttaatt ggaaacaatg ctagtgaatc tttgctagtg
    82681 atgtgtctga atacaaagaa tgaggtcatt gcttatagcg aagttttcaa aggtggaatt
    82741 aatcaatcag tagcgatacc aagagagatt tttcaactag ctttgctaaa caattcagca
    82801 aaaatcgcag tatcacataa tcatccgagc ggatatgaat ttccaagtga aaatgacaaa
    82861 agttttacta cgaatttgaa aaacgcagga aaaacaattg gtattgaatt gttagaccat
    82921 atcatcgtaa caagtaaaaa cacaagctat tactcatttc aagaaaacgg ttctttataa
    82981 aaattagaga aaagaggaaa acaagatggg cgaaagaaca caagcgttaa ttacagtcaa
    83041 atatggtaac agcaaaacga aagcaagcgt gtcgattcat tatcagtggg ggtatggtcg
    83101 atatatgctg atggacgtat tgaatcaagg aatttattta atcaataaag catacgactt
    83161 acgagataaa tcaagagaag aattagcaaa aggaatcttt gatagaagta caggggttat
    83221 caattttgcc acctatgaat caggtatgag tgcttatgat gaaacaggat ttgatgggcg
    83281 ctatgaacct tatcaagaaa cgattgaaga tgtgatgaag ttttctgata acaatgatgg
    83341 ctatgctcat ttagagatgg tgttcgatga aaatggatat agtctagatg ataaaaccag
    83401 attgcgattg tttgatattg agggtaaaca gataggacta aacaagtttt gtaagaatgg
    83461 aaaagagttt gctacaaaag attttattaa gggatacaga ttgttgctag aaaattatgg
    83521 aataaaccta gaagagagcg tgaaataaat gtttcaaaaa agaaaagaca aattccctaa
    83581 tttagaagtg aaaagtcaaa aagtgattga tggtgaaatg attgaactat tccaaaaaaa
    83641 ttatggaacg atagaaacta ccgcaagttg caaatactac tttcaaggaa agagaaacac
    83701 gtttaaaggc tttgccgatt ctgatgaaga attggcaaag aaaatcattg attggcttgt
    83761 tagcagtgaa cacgtagcaa gagaagatac cgttagaaca acgattaatg gggaagtcct
    83821 aacaggtact agatgtgttt gttgtaatca tgaatttgta gaagatgaag gttgctaccg
    83881 aatcaatgaa tatggtgatt ttatttgcca gagttgccac gagatagaat ttccaaccga
    83941 aagaagttgg tttgaataca tgctacaaga ttatgagatg gaaaaagaaa atggtgaagt
    84001 agttttttgt accaaagagg aattgagtaa gagaagcgat gatgaaattg aagattggtg
    84061 tgtgtggtta gtaggggcag atgattgcgt tgcatattac acaacagcag aataggagtg
    84121 tgaatcagca tggaaggaat ggattttcta gatcatgaag atttggttga ttttggttat
    84181 acatggaaag gaatggtagg tattagcaga agtcttgcta atgcctttta cgaaagaaat
    84241 tatgcagtat atgttcttta tgatgacgat acagaaagcc ttgttgacga ggaatataaa
    84301 ttggatttag agaatgtttt atatggaata gaaaaggaag atttagcaaa atatattttt
    84361 tcatggttgg gtcaataagt gaaaggaaga tagaaacatg acaaaattta aaagtaaggg
    84421 tatgcaatgg tgttcttatt attatgttgg aaatgatgaa gatttattga gaaaaaccca
    84481 acgaaaaggt tatccagata agatattgaa acaaataggt tattacatgc cagaagagag
    84541 gatagaaaaa gaattttgtg aggttctttt tgatagtgaa cagctattag ttttgttaac
    84601 ccaaaataaa ttgatttgcc aatttgaaaa taattaagat gaggtggtga accatgaatg
    84661 aagataagat ggtattaagt gaatatgcta gattgagaac tattaattct ttagcatctc
    84721 atttaagttg ttctttcctt acaaaggtgc taaaattaat ttacaaatgt gaagattttg
    84781 tacaagagaa gcacaaagac aagcctactg cgaataggct tgtctttttt tcattcatta
    84841 cgattccgta tgtgtgggtt agcgacaatt tgttcaatta ccgtatgata tagcatctta
    84901 atttgattag attacttgca atgtagttta ttaagagtat aatataatca taaatgtgga
    84961 taatttgaga aataatgagt gttttatact caaaaggagt ggatgaaatg aaggtcaaaa
    85021 atccaactga aaaaagcaga atccaagtcg gaatagacaa gaatttaaaa gagaacgcag
    85081 aaatgatatt ggaagagcta ggtttaaatc ctacaacagc aataacaatt ctttacaaac
    85141 aagtagtcgc tagaggtgag tttccagtag agataaagct aagtgaagag gagaagcaag
    85201 gaatcagatt gcaacagcta actaaggaca tgccagtaga tgttctagat acagatgaaa
    85261 agttagaaga gtggttcgat gaagcctaat gagatagtaa cggtctatgt tgcatttaca
    85321 gataaaaatg gtggtaaaag aagacctatt ttggtggtta gtgataaaga agatagagta
    85381 gaattttttg gaatcacaag tcaatatgag aagaaatcag accaaataaa gagagtgtat
    85441 tttcctattg gagaatggga aaaagcaggt cttaaaaagc aatcatggat tgacgtaggg
    85501 tctttaaagg ctattcccaa aagccgagag aacatatcat ttaaaaaggt tggtatgcta
    85561 tcagctagtg atacgaaaaa gctaaataag tttatcgaga gattacaaag aaaagtctaa
    85621 caaggctttt ctttttttgc ttatatgtat attaagatat acgtctatac aaacgtattt
    85681 atgtatataa aaatacacgt aaatatatac gcgaatataa acattaagaa aatattaaga
    85741 atgattaaat agtggtaatt gttaatatac gaattataag atatatttta tattgaatgt
    85801 atgtaaataa ataaaatgac tggaggatat ttaatagtga taaataacag taatctagac
    85861 ggatacagtg agtgaagtag cttgctacga acgaacaagc agaatgaaac ggtagtttca
    85921 ttagagcaag tcgcttgcga cgtagcgtta ccgctgatgt aactggcaag ttacatagag
    85981 agagtttgct tgcaaacgaa cgttacctac ttgaatagaa aacaaaaata acagattagt
    86041 gaattttaaa taggagccac aactcaacca accgcttgcg gtgggtgagt ccca
//
LOCUS       DACEDD010000005        81266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEDD010000005 DACEDD010000000
VERSION     DACEDD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.005"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IWO45_000368"
     CDS             <1..411
                     /locus_tag="IWO45_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0331872.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="IWO45_000369"
     CDS             509..697
                     /locus_tag="IWO45_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0331873.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="IWO45_000370"
     CDS             complement(755..1108)
                     /locus_tag="IWO45_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0331874.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="IWO45_000371"
     CDS             1639..2061
                     /locus_tag="IWO45_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0331875.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="IWO45_000372"
     CDS             2058..2288
                     /locus_tag="IWO45_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0331876.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="IWO45_000373"
     CDS             2786..2986
                     /locus_tag="IWO45_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0331877.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="IWO45_000374"
     CDS             3013..4206
                     /locus_tag="IWO45_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0331878.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IWO45_000375"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IWO45_000375"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0331879.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="IWO45_000376"
     CDS             complement(5898..6821)
                     /locus_tag="IWO45_000376"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0331880.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="IWO45_000377"
     CDS             7017..7622
                     /locus_tag="IWO45_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0331881.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="IWO45_000378"
     CDS             7930..8946
                     /locus_tag="IWO45_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0331882.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="IWO45_000379"
     CDS             9087..10388
                     /locus_tag="IWO45_000379"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0331883.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="IWO45_000380"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="IWO45_000380"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0331884.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="IWO45_000381"
     CDS             11117..11515
                     /locus_tag="IWO45_000381"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0331885.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="IWO45_000382"
     CDS             11581..12663
                     /locus_tag="IWO45_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0331886.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="IWO45_000383"
     CDS             12875..14437
                     /locus_tag="IWO45_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0331887.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="IWO45_000384"
     CDS             14430..15563
                     /locus_tag="IWO45_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0331888.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="IWO45_000385"
     CDS             15563..16519
                     /locus_tag="IWO45_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0331889.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="IWO45_000386"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="IWO45_000386"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0331890.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="IWO45_000387"
     CDS             17808..18626
                     /locus_tag="IWO45_000387"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0331891.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="IWO45_000388"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="IWO45_000388"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0331892.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IWO45_000389"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IWO45_000389"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0331893.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="IWO45_000390"
     CDS             complement(20506..21087)
                     /locus_tag="IWO45_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0331894.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IWO45_000391"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IWO45_000391"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0331895.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="IWO45_000392"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="IWO45_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0331896.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="IWO45_000393"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="IWO45_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0331897.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="IWO45_000394"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="IWO45_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0331898.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="IWO45_000395"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="IWO45_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0331899.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="IWO45_000396"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="IWO45_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0331900.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="IWO45_000397"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="IWO45_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0331901.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="IWO45_000398"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="IWO45_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0331902.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="IWO45_000399"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="IWO45_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0331903.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="IWO45_000400"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="IWO45_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0331904.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="IWO45_000401"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="IWO45_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0331905.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="IWO45_000402"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="IWO45_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0331906.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="IWO45_000403"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="IWO45_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0331907.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="IWO45_000404"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="IWO45_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0331908.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="IWO45_000405"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="IWO45_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0331909.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="IWO45_000406"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="IWO45_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0331910.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="IWO45_000407"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="IWO45_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0331911.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="IWO45_000408"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="IWO45_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0331912.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="IWO45_000409"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="IWO45_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0331913.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="IWO45_000410"
     CDS             33541..33726
                     /locus_tag="IWO45_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0331914.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="IWO45_000411"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="IWO45_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0331915.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="IWO45_000412"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="IWO45_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0331916.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="IWO45_000413"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="IWO45_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0331917.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="IWO45_000414"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="IWO45_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0331918.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="IWO45_000415"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="IWO45_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0331919.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="IWO45_000416"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="IWO45_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0331920.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="IWO45_000417"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="IWO45_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0331921.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="IWO45_000418"
     CDS             37801..38448
                     /locus_tag="IWO45_000418"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0331922.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="IWO45_000419"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="IWO45_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0331923.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="IWO45_000420"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="IWO45_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0331924.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="IWO45_000421"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="IWO45_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0331925.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="IWO45_000422"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="IWO45_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0331926.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="IWO45_000423"
     CDS             39886..40824
                     /locus_tag="IWO45_000423"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0331927.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="IWO45_000424"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="IWO45_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0331928.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41504..42343
                     /locus_tag="IWO45_000425"
     CDS             41504..42343
                     /locus_tag="IWO45_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0331929.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42319..43188
                     /locus_tag="IWO45_000426"
     CDS             42319..43188
                     /locus_tag="IWO45_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0331930.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43178..43975
                     /locus_tag="IWO45_000427"
     CDS             43178..43975
                     /locus_tag="IWO45_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0331931.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44178..44924
                     /gene="truA"
                     /locus_tag="IWO45_000428"
     CDS             44178..44924
                     /gene="truA"
                     /locus_tag="IWO45_000428"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0331932.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..47926)
                     /locus_tag="IWO45_000429"
     CDS             complement(45257..47926)
                     /locus_tag="IWO45_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0331933.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48164..48352)
                     /locus_tag="IWO45_000430"
     CDS             complement(48164..48352)
                     /locus_tag="IWO45_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0331934.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48622..48984
                     /locus_tag="IWO45_000431"
     CDS             48622..48984
                     /locus_tag="IWO45_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0331935.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49036..50718
                     /locus_tag="IWO45_000432"
     CDS             49036..50718
                     /locus_tag="IWO45_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0331936.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50835..52871
                     /gene="recG"
                     /locus_tag="IWO45_000433"
     CDS             50835..52871
                     /gene="recG"
                     /locus_tag="IWO45_000433"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0331937.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52920..53921
                     /gene="plsX"
                     /locus_tag="IWO45_000434"
     CDS             52920..53921
                     /gene="plsX"
                     /locus_tag="IWO45_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0331938.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54043..54285
                     /gene="acpP"
                     /locus_tag="IWO45_000435"
     CDS             54043..54285
                     /gene="acpP"
                     /locus_tag="IWO45_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0331939.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54553..55557
                     /locus_tag="IWO45_000436"
     CDS             54553..55557
                     /locus_tag="IWO45_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0331940.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55558..56502
                     /locus_tag="IWO45_000437"
     CDS             55558..56502
                     /locus_tag="IWO45_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0331941.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56502..57464
                     /locus_tag="IWO45_000438"
     CDS             56502..57464
                     /locus_tag="IWO45_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0331942.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57491..58405
                     /locus_tag="IWO45_000439"
     CDS             57491..58405
                     /locus_tag="IWO45_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0331943.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58431..60212
                     /locus_tag="IWO45_000440"
     CDS             58431..60212
                     /locus_tag="IWO45_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0331944.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60560..61246
                     /locus_tag="IWO45_000441"
     CDS             60560..61246
                     /locus_tag="IWO45_000441"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0331945.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61266..64847
                     /gene="smc"
                     /locus_tag="IWO45_000442"
     CDS             61266..64847
                     /gene="smc"
                     /locus_tag="IWO45_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0331946.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64856..65665
                     /locus_tag="IWO45_000443"
     CDS             64856..65665
                     /locus_tag="IWO45_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0331947.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65676..66674
                     /gene="ftsY"
                     /locus_tag="IWO45_000444"
     CDS             65676..66674
                     /gene="ftsY"
                     /locus_tag="IWO45_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0331948.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66861..69128
                     /gene="gshAB"
                     /locus_tag="IWO45_000445"
     CDS             66861..69128
                     /gene="gshAB"
                     /locus_tag="IWO45_000445"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0331949.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69217..69669)
                     /locus_tag="IWO45_000446"
     CDS             complement(69217..69669)
                     /locus_tag="IWO45_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0331950.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69819..70769
                     /locus_tag="IWO45_000447"
     CDS             69819..70769
                     /locus_tag="IWO45_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0331951.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70762..71721
                     /locus_tag="IWO45_000448"
     CDS             70762..71721
                     /locus_tag="IWO45_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0331952.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71718..72473
                     /locus_tag="IWO45_000449"
     CDS             71718..72473
                     /locus_tag="IWO45_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0331953.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72496..73449
                     /locus_tag="IWO45_000450"
     CDS             72496..73449
                     /locus_tag="IWO45_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0331954.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(73972..74268)
                     /locus_tag="IWO45_000451"
     CDS             complement(73972..74268)
                     /locus_tag="IWO45_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0331955.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74623..74979
                     /locus_tag="IWO45_000452"
     CDS             74623..74979
                     /locus_tag="IWO45_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0331956.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            74989..75888
                     /locus_tag="IWO45_000453"
     CDS             74989..75888
                     /locus_tag="IWO45_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0331957.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            76405..77136
                     /locus_tag="IWO45_000454"
     CDS             76405..77136
                     /locus_tag="IWO45_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0331958.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            77281..79308
                     /locus_tag="IWO45_000455"
     CDS             77281..79308
                     /locus_tag="IWO45_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0331959.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            79416..80513
                     /locus_tag="IWO45_000456"
     CDS             79416..80513
                     /locus_tag="IWO45_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0331960.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            80820..>81266
                     /locus_tag="IWO45_000457"
     CDS             80820..>81266
                     /locus_tag="IWO45_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0331961.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        26641 a        14563 c        17368 g        22694 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41461 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41521 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    41581 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    41641 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    41701 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    41761 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    41821 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    41881 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    41941 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42001 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42061 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42121 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42181 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42241 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42301 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42361 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42421 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42481 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42541 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    42601 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    42661 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    42721 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    42781 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    42841 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    42901 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    42961 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43021 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43081 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43141 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43201 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43261 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43321 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43381 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43441 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43501 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43561 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    43621 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    43681 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    43741 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    43801 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    43861 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    43921 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    43981 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44041 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44101 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44161 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44221 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44281 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44341 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44401 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44461 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44521 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    44581 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    44641 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    44701 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    44761 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    44821 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    44881 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    44941 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45001 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45061 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45121 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45181 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45241 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45301 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45361 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45421 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45481 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45541 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    45601 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    45661 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    45721 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    45781 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    45841 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    45901 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    45961 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46021 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46081 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46141 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46201 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46261 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46321 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46381 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46441 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46501 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46561 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    46621 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    46681 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    46741 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    46801 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    46861 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    46921 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    46981 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47041 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47101 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47161 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47221 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47281 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47341 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47401 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47461 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47521 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    47581 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    47641 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    47701 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    47761 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    47821 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    47881 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    47941 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48001 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48061 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48121 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48181 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48241 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48301 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48361 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48421 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48481 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48541 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    48601 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    48661 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    48721 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    48781 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    48841 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    48901 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    48961 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49021 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49081 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49141 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49201 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49261 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49321 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49381 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49441 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49501 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49561 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    49621 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    49681 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    49741 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    49801 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    49861 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    49921 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    49981 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50041 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50101 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50161 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50221 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50281 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50341 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50401 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50461 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50521 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    50581 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    50641 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    50701 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    50761 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    50821 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    50881 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    50941 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51001 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51061 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51121 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51181 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51241 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51301 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51361 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51421 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51481 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51541 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    51601 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    51661 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    51721 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    51781 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    51841 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    51901 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    51961 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52021 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52081 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52141 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52201 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52261 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52321 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52381 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52441 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52501 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52561 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    52621 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    52681 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    52741 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    52801 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    52861 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    52921 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    52981 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53041 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53101 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53161 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53221 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53281 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53341 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53401 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53461 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53521 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    53581 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    53641 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    53701 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    53761 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    53821 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    53881 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    53941 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54001 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54061 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54121 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54181 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54241 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54301 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54361 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54421 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54481 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54541 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    54601 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    54661 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    54721 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    54781 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    54841 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    54901 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    54961 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55021 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55081 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55141 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55201 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55261 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55321 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55381 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55441 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55501 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55561 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    55621 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    55681 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    55741 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    55801 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    55861 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    55921 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    55981 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56041 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56101 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56161 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56221 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56281 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56341 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56401 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56461 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56521 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    56581 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    56641 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    56701 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    56761 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    56821 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    56881 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    56941 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57001 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57061 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57121 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57181 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57241 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57301 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57361 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57421 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57481 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57541 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    57601 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    57661 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    57721 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    57781 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    57841 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    57901 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    57961 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58021 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58081 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58141 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58201 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58261 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58321 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58381 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58441 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58501 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58561 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    58621 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    58681 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    58741 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    58801 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    58861 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    58921 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    58981 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59041 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59101 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59161 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59221 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59281 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59341 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59401 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59461 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59521 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    59581 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    59641 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    59701 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    59761 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    59821 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    59881 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    59941 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60001 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60061 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60121 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60181 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60241 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60301 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60361 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60421 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60481 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60541 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    60601 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    60661 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    60721 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    60781 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    60841 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    60901 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    60961 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61021 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61081 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61141 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61201 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61261 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61321 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61381 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61441 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61501 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61561 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    61621 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    61681 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    61741 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    61801 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    61861 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    61921 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    61981 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62041 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62101 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62161 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62221 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62281 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62341 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    62401 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62461 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62521 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    62581 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    62641 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    62701 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    62761 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    62821 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    62881 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    62941 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63001 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63061 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63121 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63181 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63241 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63301 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63361 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63421 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63481 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63541 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    63601 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    63661 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    63721 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    63781 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    63841 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    63901 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    63961 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64021 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64081 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64141 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64201 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64261 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64321 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64381 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64441 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64501 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64561 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    64621 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    64681 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    64741 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    64801 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    64861 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    64921 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    64981 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65041 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65101 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65161 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65221 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65281 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65341 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65401 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65461 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65521 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    65581 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    65641 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    65701 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    65761 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    65821 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    65881 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    65941 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66001 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66061 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66121 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66181 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66241 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66301 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66361 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66421 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66481 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66541 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    66601 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    66661 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    66721 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    66781 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    66841 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    66901 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    66961 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67021 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67081 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67141 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67201 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67261 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67321 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67381 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67441 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67501 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67561 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    67621 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    67681 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    67741 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    67801 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    67861 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    67921 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    67981 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68041 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68101 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68161 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68221 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68281 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68341 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68401 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68461 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68521 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    68581 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    68641 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    68701 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    68761 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    68821 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    68881 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    68941 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69001 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69061 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69121 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69181 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69241 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69301 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69361 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69421 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69481 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69541 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    69601 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    69661 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    69721 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    69781 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    69841 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    69901 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    69961 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70021 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70081 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70141 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70201 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70261 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70321 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70381 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70441 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70501 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70561 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    70621 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    70681 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    70741 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    70801 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    70861 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    70921 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    70981 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71041 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71101 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71161 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71221 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71281 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71341 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71401 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71461 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71521 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    71581 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    71641 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    71701 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    71761 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    71821 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    71881 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    71941 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72001 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72061 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72121 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72181 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72241 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72301 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72361 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72421 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72481 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72541 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    72601 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    72661 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    72721 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    72781 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    72841 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    72901 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    72961 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73021 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73081 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73141 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73201 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73261 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73321 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73381 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73441 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73501 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73561 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    73621 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    73681 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    73741 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    73801 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    73861 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacaatgttc
    73921 aatcagctga ccttcggtat tagctcaaag cactgatttc acaacctcta tttaggcatg
    73981 tgtctcagca agcggttgat agttgatgat ctgcattttt cgataaaaat cggcgggaag
    74041 cttactccac cgttttaata attgatagaa ataactctct tctgctttga ttttatactc
    74101 gcttaatatc aatccaaaac tattcgtttt ttttgaagct aattttttat aaatatccaa
    74161 tgctttggtt ttatcttcat tgacaaagag tgtttttact tcaatataag aaccctcgat
    74221 attttgtgtt tcttcgataa aatagatgtt ttttatatat tccttcatag tgattcctcc
    74281 aaagaagtct tttatttgat aagtttactc taccgaagag ttgttaataa tccgtgaaca
    74341 aaaagtaatt ctttttccaa tagttgttaa aggtttttta ttttttatag tattttttgt
    74401 tatttaagaa aggaataagg ttaaacaaat aacaggttgc tttttgggtc tttttccgtt
    74461 caagaaactg aacgaagaaa agggaaagtt gtctcttttg tgataagaga aatcataata
    74521 aacgaatgac ttactttcca ataaattgag aacagtcaga accagacaaa ttagttgcat
    74581 ctgatgaata aagtgattat tctatgacat gaggtgatac agatgaaatt agaagtgaca
    74641 aaagaagcac aagaagtatt gaagaataaa ttagaagcag acgatcaact tttattagat
    74701 atcgaaaacg gtgacggtcc atttgcagaa agccaagtca gttgccaatt ggatacggca
    74761 tttcgattga tcattgtcaa aaaagataca caaacagatc taagtcttta ttctgtagtg
    74821 gctgatacac cagttggacc aatcaaaatc aaagacagtg caatacttta catggatgat
    74881 ccatccacac ttgctttaga accaacttat aatagtttac aattaaaagg accaagcggc
    74941 ttgagaaaag gcaacttgca agttgttcga aaagactaga gggataaaat gggaaaattg
    75001 gcgatcatca gcgacctaca tgtggacatc aataaattcg gcgaatcaga gctgatgcag
    75061 ctttggcaag ttttgcaaaa gcacaagatc actcggcttc atctagccgg agatacggct
    75121 aataaagtag accgttgcct agctgttgtc gaatttttcg tcaataaagg attatctacg
    75181 acatttcatt tcggcaatca cgagttggca gatgtgccca atgaacagat gatggaccat
    75241 tttccagacc cacatttttt aaatgaacgg tatattgctt taaatgagca gaccgttctt
    75301 ttagggatga atggctggta cgactatcag ttttccgaat tgaaagatta cgaggcaatc
    75361 gtacgaatga agcgtttgta ttggtatgat cggttcatcc agcgtgggaa aacagatctc
    75421 gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa
    75481 aaacttgagg tgattttatc cactcatttc gttcctaaac gggcatttat catgtatcaa
    75541 tctgctcctt atgagcgttg gaacaaatta aatgcatttt taggatcaga atcattcgga
    75601 gaattacttg accgttattc aaatatcaaa caagtagttt ttggccatac gcatcgtcgc
    75661 tttgaagata aatccatcgg tcacacgatt tacagctgca gaccttttgg ttattattat
    75721 gaatggcagc tgacccgaga ttttgtcttg aaagaacagt tgctagacag ctatgatcct
    75781 atgaaattac gtgtcctctt gcgtgcccat cacccagctt ttcaggccta tcaaacacaa
    75841 catttgcaag aggaatttga acaagcgatg acaatcgttc cgtattgaga cagcattagt
    75901 aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag taagagcagt agatccattt
    75961 atcgtggata tgctgctttt tttgcggaaa ataatcagcg aatgtttatt tgatcggatg
    76021 atttgtaact cgcagagtac tagatgcaag cgtgtattct ttttttgaac gataatcaca
    76081 aaaaaaaata taatatacat tttttattta attataatta atattgattg aagtctaggc
    76141 agtcaagtca cattttgata gataggaggg ggacttacga attattttaa aggctatgac
    76201 agctgtattt ctagattgtg tttgcatcaa gttgcaataa aaactagaaa tcaaatcggg
    76261 ctgataatca tttataacta atcgatacat aggaccaaat ataggtctat gttcttttat
    76321 ttcataagta tcgtaaaaca gaattttgct atggattaat ccaatcgact gatatgttta
    76381 gtaaataaag aaagaggaat gtcaatgatg aaaaaagaaa tcgtattaac agcagcaatg
    76441 atgttattca gcatggtagc aagtacgact tttgtttctg caactgaagt atacccaaag
    76501 gaatataaca cagaaggaac gattacattt gaagcaggag atgagggtgt aactcctcct
    76561 gttgatccag agaatcctga tcccaataaa cctgtcgatc catcagatcc tccaagccct
    76621 ggaacaggag gggcattatc gattgattat ggatcaaaat tcaaatttgg gactcaaaaa
    76681 atttctactg cggataaaac gtattatgca gctgctgatg tgatgaacga tgggtctaga
    76741 aaaccgacat atgtccaagt gacagatagg agaagtacct tatctgggtg gaaattaagt
    76801 gtctctcaac cagaacagtt taaaactgcc agtggggatg aactagttgg agcacagctg
    76861 aaatttacga aagggcaggc tgtttctttg gttgatccaa catatacgcc gcaaacggtg
    76921 aattctgaat taacgttgac accgggagga aacaatacgt tagcaattaa tgctaaatca
    76981 ggaacaggtg tggggacttg ggtctatcga tttggtgcaa atgaaaatga aaatcaagat
    77041 gcggtacagc tttcggtacc cggtaaatct gtcaagttag cgcaacaata ttccactaaa
    77101 ttagtttgga cattagaaga tacacctaac aactagttta ctgaaaagaa ctgttaaaaa
    77161 taaatacgta gtcatattgt cttgcgaggt aatcgagcaa aaattgtcaa aggctaggct
    77221 aaatttgttt ggatacgaat agttcatcta ggaatttaac gatcaggagg aaaagatata
    77281 ttgaaaaaga ggaggatcgt gctcatttta agcagtctct tagtgactaa tatctttccg
    77341 atcatagggt actctttgga attgaatcag ttggagcatg gaaatagaag agacgaagaa
    77401 agcaaactca cttcgacatt cgacagttta atagaatcca accagacaca agaaagcagt
    77461 gaggatttaa gtatttcgag tagtggagag accactgaca acgtagaagc tgagggttcg
    77521 gacaaacaag gactagaaga tgcaccaatt tctgcggttg agctgcctga cgatcccaat
    77581 attatttcta tcgataaaat ttttcaaaca cctatcggta ctagcacgtc catattggaa
    77641 aatgggaagt tactgcagtt gaacccagca gaaaggtctc aaagaggagc tatttggtca
    77701 aataagaaga ttagtttact ctcggatttt acatttaaat cttatttata cttaggaaat
    77761 caacgagata gagcgggaga tggcatgacc tttacattga ctggcgatcc tagaatggct
    77821 agtaatccca gtcaagttat tggatcgcca ggtatgggaa ttggctcata ttctactcgt
    77881 tctggccaac cttacattgc gaatgcgctc tcgattgagt ttgatacgta taaaaatagt
    77941 ggttcttctg atcgaatgga tcgagagata actgctgata atagagggca tggacatgtc
    78001 gcttttgtca cgcctaaacc aaataataat aattattctg gagagcatac tggcgtgacg
    78061 gtagctccta catttctttc taatggcaca tggcgcttgt taataattaa atgggacgcg
    78121 gagcaaagaa gtcttactta tgatttggaa ggtgtcggtc actcttctcg gattatacct
    78181 gatctcatta ggcagtttgg ttctacagaa gtaaattggg ggtttacttc ttcaacaggt
    78241 actttttttc aagaaaatgc tgtcgctgtc acgcgacttc catcgaatgt cacatcaaca
    78301 gctgatgtaa aagtcaacga tagtgattat ggggccatcg ctgaagctgg aggaaacgat
    78361 aagataacta tcagaaatac attgaaggtc gagggggatt ttcttgaata taagaaaatg
    78421 cagactagta tcgacctccc acaagaatta actattcccg agcatatctt gatcaatgga
    78481 aatcaaattg atgccaaaga tatcttgata accggaaatc gagtcacgct aaatctgaca
    78541 gattatttta ttgctggaga tacgttaata atcgaattgg agacaacttt gcaggaagta
    78601 attcctgaaa aagtactcac tatgcatttt gaataccttg aaaacgggaa tttgattgag
    78661 caatcgaatg aaataaacct aaagatcgca aagctgaagg agatagatat ccatgtttat
    78721 tataaagata aagacagtca gattgatatt gctgaaatga aaacaatcac tgggaaaata
    78781 ggagagaagt ataaagaaac tcctattgat attgaagggt atgtttttga ctcggattca
    78841 gcgaatacag aaggcatctt ctccgaaaat acagaggata tctattttta ttacagaaaa
    78901 ggcaaacttt atctctcaac tgctcctcaa tatgtcaatt ttggaaaaca taaaatatcc
    78961 agtagaccat taagtgaatt cggacagttt tcaggtgggt tgaaaattat agatgaacgg
    79021 gcttttggaa gttggaaatt acaattgaaa caagagaaag ttcttacaaa tggagatttt
    79081 gagcttccag aagctctttc ctttgttacc tctgaaaata caacggttat agggacatcc
    79141 gctgttacga ttttcgaaag tgatcaaaag ggtgaaagcg atttgtcctc tcttttagat
    79201 tctacagctc atcgtggaat cagaatagat attcctgtag aaaaccaaag aatcggaacc
    79261 tttgagggga aactatcttg gatacttgca gatgtcccag gaaattaaca ggaagagaag
    79321 aatataacaa aattattgag tttcaagaag ggcacaggta ccataggctc ttgtgtccca
    79381 cagagacaag attttgttat agtcttctct tacatatgat taaaggagcg attttcttga
    79441 gaccaaaaca acaattattt ataacactcc tagttatttt aggggcactt tttaacaata
    79501 tatcagtgtc agctagtgca tttaattttt cagtgacgcc gataacttca gagaatcaaa
    79561 tcgacaagag aaaaacttac ttcgatcttc aacttgatcc ggatcaggaa gtagaagtaa
    79621 aagcggaatt gagaaatgat acagaaaaag aagtgaaaat cgatatttct gtgaatagcg
    79681 caaccaccaa ttcaaatgtg atggttgaat acggaaaaaa cgagattgaa aaagacatga
    79741 gcctgatctt tgatctggta gattatgtga gctatccaca gatagtgacg ctaaaaccta
    79801 aaagtgtcca aaccgttgct tttcatgtca ggatgccgaa tgagcgattt gatggtgtat
    79861 tggctggtgg gatcacattc caagaaatcc agacggaaaa ggaccaaaca gaaactaagg
    79921 atcaaagctt atcgattgag aatgaataca cctatattat tgctttgttg atgcaacaga
    79981 cactgaacga agttgctccg aaccttactc ttcatgaagt taaaccagat caaatcaatg
    80041 caagaaatgt catcttagca aacgttcaaa atgatcaaaa aacctatatc aatcaagtag
    80101 ttattgaaac aaaaattaca aaaaaaggac attctgaagt tttatatcaa gaggaaaaag
    80161 aaggattgca gattgcgccg aatacaaatt tttcttttcc aactgcttta aatggtcaac
    80221 cgctaacccc aggtgaatat caccttacaa tgactgtttt aggaaatgaa aatgaaaatg
    80281 gaaaattttc gagaaaaaaa ggaaatacta cgatcaacta taccaatcaa tgggtatttg
    80341 aaaaagattt tacaatagac ggaaaagtag ccaaggaatt gaacacgacg gatgttacga
    80401 ttaaaaaaga caataattgg atttatatct tagtagcttc acttttctta ttgtttattt
    80461 tattatgcat acttttgttt gttttgagaa aaaaagaaga gaaagaaaag taaggttaag
    80521 ataacaaata gtaaattttt attattgtag gatggttggg ctctttttat aacaatataa
    80581 aatataccat tttattaata aaaatgcata ctagtaaatg atataattct aataacaggt
    80641 ttgatcgcga gaaaggccaa ataaaacgct ctccaaaatg ttaaaaaaaa gacaggacga
    80701 tgtatattat acaaacaaga atcgctgaca acagtgactc ggatccagga aatcagtttt
    80761 atccccagct agttggtgaa tgcatggaaa gtaatatacg tagaaagagc tgatgactaa
    80821 tgaattatga gtttttttta agcaaagaag atcacagaag ttattcatta cttaaatatt
    80881 tagaagcaag ctcaaattta tcagagtcaa tatcgaatgt tcaagaagag ttgtcgctat
    80941 cgacatttct cttgaaaaaa acgatcgata agctgaaaca tgatttacaa aagttcgatc
    81001 tagagaaaaa tttgcgagta accgtcagtg aattggatat atgtctggaa attaatggaa
    81061 aatgttctag caaagctctg ttatctcgtt atataacgga ttctttatct atgaaaatgg
    81121 tattggcttt ctttcaggaa aaatatatct cagtagaaaa ttttgctaaa cagcatcacg
    81181 tgagttattc tgtggcttat aaagtattac aaagactaaa aagaaatcta aaaaaatatc
    81241 aaattttttt tgaaaagaga aagttg
//
LOCUS       DACEDD010000006        75047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEDD010000006 DACEDD010000000
VERSION     DACEDD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.006"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1281)
                     /locus_tag="IWO45_000458"
     CDS             complement(<1..1281)
                     /locus_tag="IWO45_000458"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0331962.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNK"
     gene            complement(1865..2572)
                     /locus_tag="IWO45_000459"
     CDS             complement(1865..2572)
                     /locus_tag="IWO45_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331963.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(2637..3473)
                     /locus_tag="IWO45_000460"
     CDS             complement(2637..3473)
                     /locus_tag="IWO45_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0331964.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3491..4900)
                     /locus_tag="IWO45_000461"
     CDS             complement(3491..4900)
                     /locus_tag="IWO45_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0331965.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4914..6737)
                     /locus_tag="IWO45_000462"
     CDS             complement(4914..6737)
                     /locus_tag="IWO45_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0331966.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7017..7244)
                     /locus_tag="IWO45_000463"
     CDS             complement(7017..7244)
                     /locus_tag="IWO45_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331967.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(7580..7906)
                     /locus_tag="IWO45_000464"
     CDS             complement(7580..7906)
                     /locus_tag="IWO45_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331968.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7952..8314)
                     /locus_tag="IWO45_000465"
     CDS             complement(7952..8314)
                     /locus_tag="IWO45_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0331969.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8351..8566)
                     /locus_tag="IWO45_000466"
     CDS             complement(8351..8566)
                     /locus_tag="IWO45_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0331970.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            8693..9028
                     /locus_tag="IWO45_000467"
     CDS             8693..9028
                     /locus_tag="IWO45_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0331971.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9104..11101)
                     /locus_tag="IWO45_000468"
     CDS             complement(9104..11101)
                     /locus_tag="IWO45_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0331972.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11199..11474)
                     /locus_tag="IWO45_000469"
     CDS             complement(11199..11474)
                     /locus_tag="IWO45_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0331973.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11706..12440
                     /locus_tag="IWO45_000470"
     CDS             11706..12440
                     /locus_tag="IWO45_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0331974.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12479..13621
                     /locus_tag="IWO45_000471"
     CDS             12479..13621
                     /locus_tag="IWO45_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0331975.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13716..15107)
                     /gene="sufB"
                     /locus_tag="IWO45_000472"
     CDS             complement(13716..15107)
                     /gene="sufB"
                     /locus_tag="IWO45_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0331976.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15112..15582)
                     /locus_tag="IWO45_000473"
     CDS             complement(15112..15582)
                     /locus_tag="IWO45_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0331977.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15569..16804)
                     /locus_tag="IWO45_000474"
     CDS             complement(15569..16804)
                     /locus_tag="IWO45_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0331978.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16801..18087)
                     /gene="sufD"
                     /locus_tag="IWO45_000475"
     CDS             complement(16801..18087)
                     /gene="sufD"
                     /locus_tag="IWO45_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0331979.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18100..18873)
                     /gene="sufC"
                     /locus_tag="IWO45_000476"
     CDS             complement(18100..18873)
                     /gene="sufC"
                     /locus_tag="IWO45_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0331980.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19070..19918)
                     /locus_tag="IWO45_000477"
     CDS             complement(19070..19918)
                     /locus_tag="IWO45_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0331981.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19942..20628)
                     /locus_tag="IWO45_000478"
     CDS             complement(19942..20628)
                     /locus_tag="IWO45_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0331982.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(20621..21655)
                     /locus_tag="IWO45_000479"
     CDS             complement(20621..21655)
                     /locus_tag="IWO45_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0331983.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(22050..22388)
                     /locus_tag="IWO45_000480"
     CDS             complement(22050..22388)
                     /locus_tag="IWO45_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0331984.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22390..22746)
                     /locus_tag="IWO45_000481"
     CDS             complement(22390..22746)
                     /locus_tag="IWO45_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0331985.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(22752..23939)
                     /locus_tag="IWO45_000482"
     CDS             complement(22752..23939)
                     /locus_tag="IWO45_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0331986.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24130..24975)
                     /locus_tag="IWO45_000483"
     CDS             complement(24130..24975)
                     /locus_tag="IWO45_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0331987.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25209..26174
                     /locus_tag="IWO45_000484"
     CDS             25209..26174
                     /locus_tag="IWO45_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0331988.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            26551..27594
                     /locus_tag="IWO45_000485"
     CDS             26551..27594
                     /locus_tag="IWO45_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0331989.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(27656..28120)
                     /locus_tag="IWO45_000486"
     CDS             complement(27656..28120)
                     /locus_tag="IWO45_000486"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0331990.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            28366..29313
                     /locus_tag="IWO45_000487"
     CDS             28366..29313
                     /locus_tag="IWO45_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0331991.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(29524..31596)
                     /locus_tag="IWO45_000488"
     CDS             complement(29524..31596)
                     /locus_tag="IWO45_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0331992.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(31529..31906)
                     /locus_tag="IWO45_000489"
     CDS             complement(31529..31906)
                     /locus_tag="IWO45_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0331993.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(32035..32595)
                     /locus_tag="IWO45_000490"
     CDS             complement(32035..32595)
                     /locus_tag="IWO45_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0331994.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            32745..34037
                     /locus_tag="IWO45_000491"
                     /pseudo
     CDS             32745..34037
                     /locus_tag="IWO45_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            complement(34097..35215)
                     /locus_tag="IWO45_000492"
     CDS             complement(34097..35215)
                     /locus_tag="IWO45_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0331995.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(35229..36650)
                     /gene="arcD"
                     /locus_tag="IWO45_000493"
     CDS             complement(35229..36650)
                     /gene="arcD"
                     /locus_tag="IWO45_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0331996.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(36896..38017)
                     /gene="mnmA"
                     /locus_tag="IWO45_000494"
     CDS             complement(36896..38017)
                     /gene="mnmA"
                     /locus_tag="IWO45_000494"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0331997.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            38421..39020
                     /locus_tag="IWO45_000495"
     CDS             38421..39020
                     /locus_tag="IWO45_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0331998.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(39158..40444)
                     /gene="efmA"
                     /locus_tag="IWO45_000496"
     CDS             complement(39158..40444)
                     /gene="efmA"
                     /locus_tag="IWO45_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0331999.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(40715..41059)
                     /locus_tag="IWO45_000497"
     CDS             complement(40715..41059)
                     /locus_tag="IWO45_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0332000.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(41196..42332)
                     /locus_tag="IWO45_000498"
     CDS             complement(41196..42332)
                     /locus_tag="IWO45_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0332001.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(42521..43495)
                     /locus_tag="IWO45_000499"
     CDS             complement(42521..43495)
                     /locus_tag="IWO45_000499"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0332002.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(43575..44450)
                     /locus_tag="IWO45_000500"
     CDS             complement(43575..44450)
                     /locus_tag="IWO45_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0332003.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            44580..46487
                     /locus_tag="IWO45_000501"
     CDS             44580..46487
                     /locus_tag="IWO45_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0332004.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(47028..50306)
                     /locus_tag="IWO45_000502"
     CDS             complement(47028..50306)
                     /locus_tag="IWO45_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0332005.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(50577..51122)
                     /locus_tag="IWO45_000503"
     CDS             complement(50577..51122)
                     /locus_tag="IWO45_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0332006.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(51134..51826)
                     /locus_tag="IWO45_000504"
     CDS             complement(51134..51826)
                     /locus_tag="IWO45_000504"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0332007.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(51845..52129)
                     /locus_tag="IWO45_000505"
     CDS             complement(51845..52129)
                     /locus_tag="IWO45_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332008.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(52142..52705)
                     /locus_tag="IWO45_000506"
     CDS             complement(52142..52705)
                     /locus_tag="IWO45_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0332009.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            52933..53595
                     /locus_tag="IWO45_000507"
     CDS             52933..53595
                     /locus_tag="IWO45_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0332010.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            53719..54915
                     /locus_tag="IWO45_000508"
     CDS             53719..54915
                     /locus_tag="IWO45_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0332011.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(55016..56392)
                     /locus_tag="IWO45_000509"
     CDS             complement(55016..56392)
                     /locus_tag="IWO45_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0332012.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(56429..57295)
                     /locus_tag="IWO45_000510"
     CDS             complement(56429..57295)
                     /locus_tag="IWO45_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0332013.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(57384..58409)
                     /locus_tag="IWO45_000511"
     CDS             complement(57384..58409)
                     /locus_tag="IWO45_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0332014.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(58427..59101)
                     /locus_tag="IWO45_000512"
     CDS             complement(58427..59101)
                     /locus_tag="IWO45_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0332015.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(59285..60178)
                     /locus_tag="IWO45_000513"
     CDS             complement(59285..60178)
                     /locus_tag="IWO45_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0332016.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(60252..61949)
                     /locus_tag="IWO45_000514"
     CDS             complement(60252..61949)
                     /locus_tag="IWO45_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0332017.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(61942..63255)
                     /locus_tag="IWO45_000515"
     CDS             complement(61942..63255)
                     /locus_tag="IWO45_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0332018.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(63255..67292)
                     /locus_tag="IWO45_000516"
     CDS             complement(63255..67292)
                     /locus_tag="IWO45_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0332019.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(67305..67763)
                     /locus_tag="IWO45_000517"
     CDS             complement(67305..67763)
                     /locus_tag="IWO45_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0332020.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(68061..68579)
                     /locus_tag="IWO45_000518"
     CDS             complement(68061..68579)
                     /locus_tag="IWO45_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0332021.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(68581..69198)
                     /locus_tag="IWO45_000519"
     CDS             complement(68581..69198)
                     /locus_tag="IWO45_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0332022.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(69195..70049)
                     /locus_tag="IWO45_000520"
     CDS             complement(69195..70049)
                     /locus_tag="IWO45_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0332023.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(70046..70957)
                     /locus_tag="IWO45_000521"
     CDS             complement(70046..70957)
                     /locus_tag="IWO45_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0332024.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(70998..72332)
                     /locus_tag="IWO45_000522"
     CDS             complement(70998..72332)
                     /locus_tag="IWO45_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0332025.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            72540..73370
                     /locus_tag="IWO45_000523"
     CDS             72540..73370
                     /locus_tag="IWO45_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0332026.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(73614..73863)
                     /locus_tag="IWO45_000524"
                     /pseudo
     CDS             complement(73614..73863)
                     /locus_tag="IWO45_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(74054..74248)
                     /locus_tag="IWO45_000525"
     CDS             complement(74054..74248)
                     /locus_tag="IWO45_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332027.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(74753..74938)
                     /locus_tag="IWO45_000526"
     CDS             complement(74753..74938)
                     /locus_tag="IWO45_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332028.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT        21765 a        15928 c        13122 g        24232 t
ORIGIN      
        1 tttattttga tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg
       61 agtttgacga ttcttcttca acacaatcac cgtcgttgga atcgatgtcc caaagaacaa
      121 gttcgcaggc ataccgatca cagcataaat actgccatct tctagtaatt tttgacggat
      181 gaccccttct gcagcgccac ggaataaaac tccgtgtggt aagacgatgg ccatggtacc
      241 tgtttccttc aaatgataga acccatgaag aagaaacgca aaatctgctt tggattttgg
      301 cgctaatttc ccataacgat taaaccgaga atcatctaag aaagtcgtat ctgctgacca
      361 attagccgaa taaggtgggt tcattactac tgcatcaaat gtgtaaggtt catctgttgg
      421 ccagtcctta ttcaacgtat ccccattgcg gagattcatt tcttcggcgt cgacaccatg
      481 taaaatcaag ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata
      541 tttaacgtta tctgggtgtg ttaagtagtt acgtacgttt aacatcaatg aacctgaacc
      601 catcgttgga tcaaaaacac taaagaatcg tcgttccttt tgatcgagcg ttacgatttg
      661 cgccatcata tcagaaacca tatgaggtgt gtaaaattct ccggctttct tccctgcttc
      721 tgaagcaaat tgactgatca gaaactcata cgcatccccg atcacgtccc catcgtgttc
      781 taaaacatct acatcattca atttcttgat gacttcggta atcgtcacat ttcgttgttg
      841 ctcatctgtt cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg
      901 attgtaggta gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc
      961 ttgttttgct tgatccgcta agacgttgaa aagatactct ggtgcaattg catatcccaa
     1021 aatatcaacg attgtggcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
     1081 atacaacatt gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
     1141 aaccacttgt tccaacaatc gatcggataa atatttatag aaaatcagtc ccaataaata
     1201 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataattt
     1261 ttgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
     1321 ccctcttatt ttagcatacc cccttttata aaaagcaaaa aaaaaagaaa gaaacttatt
     1381 cttgctaata aactacgcga aaggagtcta acgttgtttg atataactta gactgatcgt
     1441 taacttattt tttatatttc tacatagtta tctttagaac catttcagat acatatgaaa
     1501 caattacata tgattttttc ataaaaaaac agtactgtct agtagtataa aatatacaaa
     1561 tttcttaaaa attttttcac taaaatcact acctgaaaca gtctaaaaca cttatatatc
     1621 aacgtttaat gaaactatct ccgtttcatt tcagatacaa ataaatatag tcgggctcca
     1681 aatccgaaaa aaaatacccc cagtagctta tcctaagcta ctggagatgt ataagtacgg
     1741 tcatataaca acggggtagg gtaaataaat gaaaaagatg gaagaatcat tattcaataa
     1801 cttttttaaa tacttcgttg taatcagtta cctgtccgtt cttaaatacc acagaataat
     1861 ctttttaccc attttcaagt aaccgaagtg gaatgtcttt accaacttgc tctgtcactg
     1921 cattactgat acctgttaga ttagctaata aatctgataa agattgagca tctgtagaaa
     1981 tctctgtatc agtatttatc gtgattcctt tatcttcatt tacaagcttc caagtaggtg
     2041 gtatagctgt gtatgtttcg atgaatgaat caattctttc atctatcgtc ttcccagtat
     2101 ctacttctgt ctcattactc ttagatgaat cgattctttc atccatcgtc tgctccgtaa
     2161 ctgcttctgt ctcattattc tcaccggaaa ctctcttcac acccaaactt atatattgtc
     2221 catctttagt gtgcatagtt aaaattccca cgcttttact aataaattca ctataaccag
     2281 ttctttcaat agagacttca atgtatttca cactatcata ctcaaaagaa aaatagacat
     2341 gtaaaagcat tttgttgttt acatctacat aataatcctt tgactctact tgagaccttc
     2401 ctatacggaa tacagcttta ctgtctgata tctctatacc agcatcctta ttattaatct
     2461 gccaatctcc aagtagctct tctggcaagc tagtagtttc ttgtgaacac tttaccactt
     2521 cactggtaga ggtcgaaaca ttcacctccg cactgttaga agaattttcc aaggaatcag
     2581 gagccttagc acaaccaaca agtctttata tattgtgcag ccgatatttt cttcattcag
     2641 tacacccttg aaatatgcag tgttagataa agcagttcgt cctgatttgc tgaggcaccc
     2701 atctcttctt gaacgtactc cttgattttc aaggccgccc gatactcttt cggaaaacgc
     2761 tgcttcactg aagccagcaa aaaatcatcc ttctccggca actcttcctt cttaatcatt
     2821 cgggcaataa aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga atctttatca
     2881 accttttttt cgggaaacag atatcctagc atagccaaga tatcctttag tgctcttacc
     2941 gagagcatgg tttcttccat atttgcttgt tcactttggg catttacaat gtgaaacgca
     3001 atgtttccag cctcctcttc tggcaactga acattcattt ttttgttgat caattggata
     3061 gcatagcagc cgatctcaaa ttcttttggg taatactttt gaatttcaat cagcaggcga
     3121 ttttgaaaaa caatgccttt ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag
     3181 gtaaaaaata gacttttatt cagcttaaag tcaaacttgg cgttggcata atcgataatt
     3241 cgcttgacgc aaataattag atcatccgga gcagcctcaa acagtttaat gtaatccttt
     3301 tttagagcat cagaaagtga atctaattta aatacttttt ggattttgtc agccggaacc
     3361 aaatcgcctt tctttagttg aaatccaatc cctttcccca tcaaaattac ttctttgcct
     3421 tgcttatcta agcaaagaat taaactgttg tttaacgttt taattactct cattagattc
     3481 accgttctct ctaagctaag ccatttcttg cgattacttt ttgataccaa aagaagcttt
     3541 ctttttttat tcgttttaat tctttaagat tctcatcggt tcggtcaaca tatataaaac
     3601 cataacgctt attgaagcct tgatgggtac taacaacatc aatcgctgac caaggtaaat
     3661 aaccaaaaac atctacacca tctgttaaag ctgcttgaat agccagaata tgtttggcta
     3721 gataatcaat tcgatcctgg tcattaattc gtctatcttc ctcaactatg tcgggtgccc
     3781 catagccatt ttcagtaatt aaaataggta aatcatagcg atcgtacaat ttcctcaatg
     3841 ttaatcgtag tccagtaggg tcaactaccc agccatatgg cgttttatct acataaggat
     3901 tttcagctgc ccggtagaca ccttcttctc ccaacatgat ctgttgatcc cccgctctag
     3961 cggaaacatc tgatccgtca tttttgctgg cagcgatggt ggcagttgaa taataattaa
     4021 tcgcaataaa atcgggttta ccggaggata aaattgcctg atcctctgcc tcaattttag
     4081 gatataatcc acgatcattc aaatagtttt caaacaaata attgtatttt ccccgaactg
     4141 ctacatctaa gaaagaccag cctctcaatg tttcccaatt atgggctgca attgcatctt
     4201 ctggactgga ggtagcttga tacatagccg ttaaattgat tgctggaccg atttttcctt
     4261 taagattcaa ctgatgatac aagtgaatta ctctagcttg agctaataaa acatggtgat
     4321 taatttgaaa gagttctttt tttgaaggca gctctccact tggtggcaaa ccaatcgctc
     4381 caggatgaag gatcatcgtg ttttgctcat taattgtcag ccaatagttc accttgtcac
     4441 catactcagt aagcaatacc tgtacatagt ccacaaaata atcgatagtt gctcgattta
     4501 accaaccgcc ctgctcctct agaaaataag gtaaatcgaa atggtacatg gtgacaatcg
     4561 gttcgattcc ctgattcagg cattcatcaa ttacttgatg ataaaaagaa attccctttt
     4621 gattggtttt attattttca atatccataa ttcgcgtcca agcgatggaa aagcgaaaag
     4681 cttttaagcc taattcttta aacagctgta tatcttcttt atagtgatgg taaaaatccg
     4741 aggcaacttc aaaattagct accccttctg tatgctgata cttgtcaata atagacggtt
     4801 gtttcccatc ctcctgatag gcaccctctg cctggtaggc actaatggag gcaccccata
     4861 aaaacttctc tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc tccttaacta
     4921 atttgcatca atggttcgtg ttcctttaag cttccgcttg cttcactaat tcctgcgtaa
     4981 ttttcactgt tggtcactac cacaatcact gtatcatcgt agccagcgtt tctaatggca
     5041 gcgaaatcgg cgttgattaa taattgtcct gctgacacct gatctcctgc ttttacaaat
     5101 acttcaaagg gttccccttt taaattcacg gtgtcgatcc ccacatggat caataattca
     5161 ataccttctt tagaacgtag accaatcgca tgtttagatt ctaaaatagt aatgacttct
     5221 ccctcaacag gtgaataaat gctgttttct tctggatgaa tggctacgcc ctttcccaat
     5281 agtcccttag aaaagacagc atcgttcact tcagaaatag gacacacggt tccttttacc
     5341 ggattttcaa caatgactgc ttcggctatt gccgtttgct ctgattcatc agaagtatcg
     5401 tcaaatccaa ttagataagc aatgataaag gttgcaacaa aggcgattac acacgtaata
     5461 attgcgtgaa caatattcat tggattttct ccaataaagg ctggtaaggc tgctaagcca
     5521 ggggaaacaa aggcatatct gactaaaccg ctcaagccag catacaaacc agcaattgca
     5581 ccaccaatca tggaagcaac taatggtttt ttcagcttca aggttactcc atagagtgaa
     5641 ggctcagtaa ttcccattaa agcagtgatc ccagtggaca cagctagctg cttcaactct
     5701 ttgtttttag ttttcgcggc tacagcaaag gtagcacctg attgagcaat attagaagcc
     5761 agcattcctg gcccattgat catttcagaa cccgtactgc tcaattgcat agtagcgatt
     5821 ggcgtcattg cccaagctgt acccgtaacc accaaaaatg gctgcaaacc acccatcaaa
     5881 aatggaatta accaagctgc tttgccgtta atccattcag cccccattgc tataagatcg
     5941 ttcagcaacg ttccaaacgg tccaatgaca accaacgcta aaggccccat aattaaaaat
     6001 accagcatcg gcttagtaaa gaatttaata aacgaaggcg aaattcgatc cgcaaagggc
     6061 tctacatagg acatgatcca aacagtaaag ataatcggta aaactgatcc cgcataatct
     6121 gcagctaata ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa
     6181 atatttggat gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt gacatcaaac
     6241 tttatggctg ccccataggc taatagtact ggcataaaat aaaaggctgc atcagcaata
     6301 gtattcaaaa tgatgtaagt ttgactactg tctgatagta caccagtcaa agtcaagacg
     6361 gccaaaatgg cttttaccat cccggcacct gttatagctg gaatcatcgg tgtaaaggtg
     6421 gtagaaatga cactaatcaa tcttgaaata atactttctt gattgtctga agaggggccc
     6481 tttttaattg aagttcccac ttccttttga atttccttga aggctgtttg gacttcattg
     6541 ccaatcacca cttggtattg tccaccgcta tccaccacac taattacacc aagtgttttt
     6601 tctaaggctt tcgtatcggc tttcctttta tctttgagtt caaaacgcag tctggtggca
     6661 caatgggtta agctgaccac attttctttt cccccaacct gttgcaatac atttcttcct
     6721 acttctctgt aatccattat tctctctcct taaagcagta aaaaagacca agtcaaacca
     6781 cgccccaatc aacattgaag tgttagtttg tcttggtctt gcctgcataa tcagtaacat
     6841 gccaataacg ttattttgct gtaatccaat aatagcacat tatgatagcg caatcaacag
     6901 ggaatttaat tttttatctt ttcgtttgac cgtatagggt aatataacag ttagatcaca
     6961 tcaactaggg tatatctgcg tatacttact gctaaaataa gtatatctaa tattaactat
     7021 accagtaaaa ccattcctac ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa
     7081 aatgcatata acggatcaca caacttcaac aattctgctc ttgggaaaaa ttgatgaatt
     7141 tggtgagtaa aggatggaat aaacagcatt ccaacaatat aggcaacaat agtgatactt
     7201 gtttttttgg taaaaatcag taagcaatag ccaatttcaa tcattccgag atataaaggt
     7261 atcaatagaa tgatttgggc tagtattctt aaaaattaac tagcaaatgt ttaaactgat
     7321 ttatttctaa tctacatgta gtaagtattt caattccacc aattatcaat aaaaatacca
     7381 aacatatcca taataatttt ttctttttca tttcttttat attgttataa cagtgaataa
     7441 cataaaaatt cccgacaatc attgtaatat ttttttgtca cctgtattag tatttaattt
     7501 ataggaaact ataaaaactc cctagtgaat ttacaccttt gggagttcct gtttggacgg
     7561 tatgcataat acatttaaat caatttaaca ttaacacctc agcatatggt atctccacat
     7621 atatacggtc tttctttgga tagtactcaa tatttatgtt gatagcactt ccattgtcta
     7681 atatagcttt agcccggtat gtgttatctc cattttcatg agtaatcgtc acatcatcac
     7741 atgatcgatt taagttgtac tgttcttgta aaatctgtgt taccaatggt tttgatgact
     7801 tctctaatcc agatgtacct gtcatttttg aaaaaataat acctataata gccaagacaa
     7861 caatacctat aatgacaaac ttcttgttca tatttacttt attcaacttt ttatctccta
     7921 tctccaaaat aatttgttct tatccctctt atcagtacat tgacatccct aaaagtacac
     7981 caaagattgc tccagctacc cctgctatca tcaaaatcgt acccattttc tcatcttttt
     8041 ctcgatataa gtagcccata ataacagcaa cagcaccaaa tacaacagga ataaatgcta
     8101 acgatactac catagaaatc caaccaataa tcaaatacag cttccctgtc tcttgtccat
     8161 gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt actgtgtctg
     8221 ttccggttgt ctcatctgtt tcttgtagtg ctccacaaaa ctggcaatac ttactttcat
     8281 ctgttatttc tttgccacat tttctacaat acatgtcttc tcctcctaaa atcattcaat
     8341 tgcttattag ttaactgttt actttattaa atacatcaga aaacagctca ttcattggta
     8401 cacttaatat ctcagaaagc atcttcattt gatagggttt aggaatcgtt ctccctgttt
     8461 gccacgaact tacacattgc tgtgtaacat tcattttttt tgctaactcc gtttgagaaa
     8521 gtcctttttc ttttctgtat cggctgagat tacttacttt tttcatgatt ctcccctcct
     8581 gttttattat atacaacaat atgatgtatt tcaactcata aaatataaaa aacttcaaat
     8641 accattacaa atacaacaac ttgttgtact atactgagat gggggtgtat taatgaaaca
     8701 gaaacaatat gcaaaggtca ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga
     8761 attagctgaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa attctgtaac
     8821 agagcctaac ctcgatagct taacagctct ttctagagcc ctaggaaccc ctgtaagcca
     8881 ttttctagac aatgttgtcg tagattatga ggaagagttt ttggctctct atcgttctct
     8941 tagcactgaa gatgcagttc gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa
     9001 tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact ttaacggtgg
     9061 gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt aatacaattg
     9121 ggtcttttct cttccttact ctccgtccat catctcttgt tacacttgta aattgataca
     9181 gaggaactat cccagctcct gataatatca attcttttat tgtttcatct atccctgccc
     9241 ctttcgctaa atatattaaa aaactactac ttgctttcac attgtatagt ttcttaatat
     9301 ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg ttttttatta
     9361 aagcattttt cacgctgtta tccccaacat tacgtaggcg ttcacaaaca gtgttaataa
     9421 gttctttctc tgtcattaga tatgtccctt gccacgataa gccctgtaaa acttcatgtg
     9481 cttcatctaa aatcaattgt ttttccttgc tgagttctga tcgggaattt tgaggaaata
     9541 ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa aaatattgat
     9601 atgtaaaatg aattttttcc agaagtggaa ggatatcttc attcaatttt tgaatcacag
     9661 aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta aagtttccta
     9721 acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga acatcttcat
     9781 catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta ttacatttat
     9841 ctatgtcata agttttacca tagtaagaac tcattttttt agctaattgt tcccccgaca
     9901 aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa agcaagacat
     9961 catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt ttgtctttca
    10021 ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga aaatgtgtag
    10081 gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg tctttcttta
    10141 aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg gaatgacaat
    10201 aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta tgatcgttgt
    10261 gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca atggttaaca
    10321 atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag tctttaaacc
    10381 actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg gtttcattga
    10441 ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc atggtaaaat
    10501 caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc actgcatctg
    10561 acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca cacccgggga
    10621 tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta attgcttctt
    10681 ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata cttttttgta
    10741 tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg cttaagtatc
    10801 tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca gtgttatcga
    10861 catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct ttattctttg
    10921 tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca tctcttgtaa
    10981 cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt tttgtaactg
    11041 gcttctctaa taatttttct tgatctacat aaattggaat attaatcatt gatgtttcca
    11101 tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata ataaaaacaa
    11161 actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata ccgttccatg
    11221 aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat aacaggtaac
    11281 ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata ttcacttgcc
    11341 tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag cataggcttt
    11401 agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat gctatcaatg
    11461 atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt tctaagaatt
    11521 atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta atcaaacaaa
    11581 aaatgattac cagttcatcg acatacacat aatacctcga taaaaaaggg gtgtcaatag
    11641 ttttgtaaaa tttatttaca acaccccttt tttgaggtat actaatttta ggaggtgtgg
    11701 tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa
    11761 aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta
    11821 acaacggtca attaattgac aaaaagacta tctacgaatg ggagaaggga acctatttac
    11881 caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag
    11941 tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac
    12001 tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac
    12061 cagtacactc taatttagat acatggctgg atagatattc caaattagaa ccagagatgc
    12121 aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca
    12181 atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca
    12241 ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac
    12301 ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata
    12361 taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga
    12421 agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttcat
    12481 ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca
    12541 cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag
    12601 caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa
    12661 aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg
    12721 taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact
    12781 tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt
    12841 agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat
    12901 tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct
    12961 tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa
    13021 tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt
    13081 ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac
    13141 gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat
    13201 aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct
    13261 ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa
    13321 tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa
    13381 cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc
    13441 tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt
    13501 agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat
    13561 tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta
    13621 aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc
    13681 cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc ttccatctca
    13741 taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt ggtgaatggt
    13801 tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc tcggctcatc
    13861 agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc taaagagact
    13921 tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc gtccatgatg
    13981 atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa ggttacttgt
    14041 cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat agagctagat
    14101 gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt tgctcctgca
    14161 aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac acttgggtat
    14221 ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc gccttcgtat
    14281 gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg gatcgttgtg
    14341 taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg caggctgtta
    14401 cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca
    14461 atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt
    14521 aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc tgaccatacg
    14581 gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga aaagtactct
    14641 ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac gattcctaac
    14701 ttttcgaatt catctttcat attatggtac acgacttctg attcatattg tgcggaagca
    14761 ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt atctttgatt
    14821 ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg
    14881 atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg cattttgtta
    14941 aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc tttgattctt
    15001 gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac cggttcgaca
    15061 tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt ctcactcctc
    15121 ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt ttcctagtgc
    15181 tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac caccaagcat
    15241 agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa gctgtagaaa
    15301 gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt ctgtcatcat
    15361 cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat ttttgatgat
    15421 tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt tgttcaattc
    15481 gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag aatgatccaa
    15541 gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa aactcctttg
    15601 ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc
    15661 tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca caatgatgac
    15721 ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc
    15781 catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga ccataaacag
    15841 tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct tcttcatatc
    15901 gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct aatgcgatag
    15961 cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc catgtactgt
    16021 cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc atttcttcta
    16081 accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc
    16141 tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc ggggcagatt
    16201 gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct aattcttgaa
    16261 caggattgaa gacacctaag acattggata catgagcaat ggagacgatt ttggttttgt
    16321 ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt agttcgatat
    16381 atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca ttagaatgat
    16441 gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg ccgaaggttc
    16501 tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt
    16561 ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt
    16621 ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta
    16681 atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga taaactaaag
    16741 gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt ttagcatcga
    16801 tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt gttctacagg
    16861 gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg cttcttcttt
    16921 atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc ctacactggc
    16981 agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg catcaccacg
    17041 tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac cttttgcccc
    17101 tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga tcactccgtg
    17161 ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat cgattccttg
    17221 gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac cttctcctgc
    17281 taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa ttgcccagtc
    17341 gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa tattttctcc
    17401 taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga cttccacgat
    17461 gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt ccagataaga
    17521 aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct catcggatgc
    17581 gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt taggcacata
    17641 caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt cttcgttcat
    17701 gtttactgct tttttcatat aatattcttg gactagttct ggatattcga tcattgcagt
    17761 gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt gttcaaaaat
    17821 cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa atgccggaat
    17881 cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc agcgatggaa
    17941 tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg
    18001 gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta ccgcgtcaag
    18061 ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt cttctttata
    18121 gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac gtttagctag
    18181 gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga catcaggtgt
    18241 gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg
    18301 catttcattt actcctttag caacgacttt caaagcatcg atatccagac ctgaatcgat
    18361 ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct cattccgttt
    18421 cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt ctggcatatt
    18481 tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt
    18541 ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga tacctggtat
    18601 ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa
    18661 gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat agtttggatt
    18721 gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca tgatggcatg
    18781 gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt cctcgatcga
    18841 cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat tctctttcgt
    18901 tctcactcat tttagactaa ttgagaatga agcgcaatga ccttccttac taaaatcaaa
    18961 tatcttttat ttattattaa acaattttga tttctaaatt agaataggta taaaaaagat
    19021 gagaaataag ccattacaga cttatttctc atcttttttg ttccatacct tattttgcat
    19081 cagcctttac tggtttaaca gagccgcccc atttgtctgt gatccattct tggacttctt
    19141 tgcggtgaag cacttcaatc agttttttga ttttgtcact gtcttcatca cctttacgag
    19201 tcgcaataat attcacgtaa ggagagttgt ccttttcaag caatacggca tcttctaatg
    19261 gattcaaccc tgcgccgtaa gcgaagtttg cgttgattgc aactaggtca ccttcatcat
    19321 tttggtacgt agaagctaaa agtgatgggt cgatcgtatg gttgaacttc aagttcttag
    19381 ggttttcttc aatatcatcg aatgttgctg tttccagatc tactccatct ttgactttga
    19441 ctaagccagc atcttgtaga attgtgatga tgcgtcccca gtctgattca gaatttgaag
    19501 taatgattgt tgcaccatct tttaattcgg aaacatcttt gactttttta gagtacaagc
    19561 ccattggttc gatgtggaca gcaccggcat tgacaaagtc atatcctttt tctttgattt
    19621 ctttgttcaa ataaggaata tgctggaagt aattggcatc aatgtcgcct tcttccaaag
    19681 attggttcgg taatacatag tcatcgaatt tgacgatttc aagatcgatt ccttcatctt
    19741 tcaataatgg tttcacatgt tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt
    19801 tcaatgtcgt gtcgtctgct ttatttgctg aatctttgct gtcgctgttg ttaccgcatg
    19861 ctgctaatcc tacagtcagc aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat
    19921 cctacatccc cctatatcct attatctttt gtctgttttc ttagttaaga aatcaccgat
    19981 tccttgaacg ataaatacga ttagtaaaat aattaaagtc gcaacgaatg tcactgttag
    20041 attaccacgc tggtagcctt cctgccaagc aagacctcca agtccacctg cgccgatcgc
    20101 gccggccatc gcagtaaatc cgatcatcga gatcgttgta actgttaatc cagaaatcaa
    20161 tgcgggaata ctttcaggta tcaagacctt ccagatgatt tcccagtaac ttgctcccat
    20221 tgcatctgct gcttccagta caccagggct gacttcacga aaagcaatct ctactagacg
    20281 ggcataaaat ggtgctgcgg ataaaacaag agctggaatg gctgcttgcg cacctagcat
    20341 agtgcctacg attgctttag taaatggaat gatcaaaacc atcaggatca taaatggcgt
    20401 tgaccggaaa atattactga tgatcgatac gattccataa atgattcgag catacggttt
    20461 gtttttgcgt ccaatagagt aaaggatcaa tcccaaaact aatccgatga tcgtcactaa
    20521 gaccattgag attaatgtca tatatagtgt atcaaggata gcctgattca tcgtttccgg
    20581 gttgatttgt ttaaaatcta gatatgtttc tgaaaagctt ttatccacga tggatcacct
    20641 caattcctac tccttgttct ttgatgaaca cttctgcttg atctaattct ttggtttcac
    20701 cagtcatcaa gacagtcaat gaaccaaagg aaccttcttt cacttgtttg atctttccat
    20761 acacaacgtt gacatcgatg ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg
    20821 catttctttc gctaaaggtc aaagttgcca aacggccgct tggattttct ttgatgagtt
    20881 cagctaatag ttctgtcgaa tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg
    20941 ttacttcttt tttcggttgt ctgaagacag tcaatacgtc tccttcttct acgactttcc
    21001 ccaactccat cacagcgact ttgttgcaga ttttgcggat cacattcatt tcatgtgtga
    21061 tcaagacgat ggtcaaattc aatttcttat tgatcgctaa caataagtct aatacttctt
    21121 ctgttgtttg cgggtccaat gcgctagtag cctcatcaca aagcaggatc tcaggatcat
    21181 ttgccaatgc tcgtgcgata ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat
    21241 atgcttgtcc gcgtccttct aacccgacta aacgcaacag ttcttctgcc cgttctttac
    21301 gtttgctctt tggtacgcca gctaattcca gcggcagctg gatattttca agtacagttc
    21361 ttgaccataa caggttgaaa tgctggaaga tcataccgat tttctttcgg aatgtacgta
    21421 attctttgtt ttttaatttt gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg
    21481 gaagctcaag tccattcagg agacggacta atgtactttt tcctgcgcca gaatagccga
    21541 cgattccata aatatcccct ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt
    21601 ttccgttttt cccattgaat gtctttttga cattcgttag ttggattaat gacatgccat
    21661 tcacatcttt ctcttataat ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa
    21721 aaggacgaaa gcttgaactt ccgtgttacc accttgattt accgttactt cgcaataaca
    21781 gcctcttcca gtacttggac ttagcttctt tccgtatact gtcgcgctgt aacgggcact
    21841 cccgtagtaa gtttgcttac gctcgcttac tctgctctaa gaccatcttc catctcattt
    21901 tcgttaccca ttttcagcaa acagggctct cttcaaacgt ccgtgacatg tactcttctt
    21961 ttcaaagctt tctcgtttca tataggattg cttatgatta tagcaactgg aagaatcagt
    22021 gtcaactttt ttcaagtgat tttctgattt tataattcgt caaattcttt tggatcaaca
    22081 tctttgaaac ttaagatcca cgcattcatt tcatctgaat cattcaagat attgatatct
    22141 tcaccgattt tatcattaac agaagaaact gtacccgtta gcgggctggc aaattcattg
    22201 accgcttttt ccgcttcaac ttcgatcaga ggttcatctt tttttagcga ttgtcctgct
    22261 tttggcagat tggcaaacgt gatgtctcca agttcttctt gcgcttcatt tgtcaaacca
    22321 acacagtatt cttttccatt aaataagatc caaaggttgt cctttttctt caaatgcttg
    22381 cctgccattt tatatcagtt ctccttcata aattttttca ttgaatccaa ttgcaacaag
    22441 ctggtcctct cgtaagatca gtggtcgctt gatcagcata ccatctgtcg ataagacttg
    22501 gctcgctttt gaagccgtaa actcgcctac ttgatctttc agtccaagtt cccgatattt
    22561 catcccgctt gtgttgaaca ggcgacggat tggtacttga ctcgtttcta gccaagtctc
    22621 aatcgttgag gccttcggta cttctttgat catatcaacg gtttgataac taacgccatg
    22681 ctggtctaac cacgctttgg cttttttgca tgttgaacat ttcgggtacc agtaaaaggt
    22741 gtacaatttt cttacctcct tgaagatctt gcaggtgctt cattttggta tctcatcgat
    22801 aatatcaacc cgataccgat catgttccct aaaatcgagg atcctccctg actaatgaaa
    22861 ggcaatggga tccctgttaa aggaagtaat ccgatatttg ctccaatatt ctcaaagaca
    22921 tggaacagca gcatcatgat caaaccgctt gcgatatagg cgtaaaattc attattcgta
    22981 tcgaaacaca cccgaatcat acgatagatt aaaataaaat acaagaaaat gacgaatgca
    23041 ccaccgataa agccgaagtt ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca
    23101 ggcacataga tattgcttac gttgaatcct ttaccaaaca agccaccggt accaattgcc
    23161 aaaagtcctt gggccggctg ataactcatt cctgacgtat catggaaagg gtccagccat
    23221 gaatcaatac gtgcaaactg ataagatttg aatccaactt tataaagcaa gtctcttccg
    23281 ccttcagtcg taaccaagaa gatcgttcct gctccaacga ctactgccaa gattactaca
    23341 ggtgcgatga tgcgccaaga gattccagac atcaggaaaa tcccgccaaa gatcgcaaga
    23401 aagacaagca tcgttccaaa gtcatcttgg gccatgatca atgctaaaac cggtattgtc
    23461 acgatcaaga gcttcccaat cagccaaaag tccgatttca tcgtccgttc ttttacttgc
    23521 gtattatgtt tagttacgat cagtgccatc atcaagatgt acgcaatctt catcaactct
    23581 gctggctgga aagtaaacgc accgaatcga aaccagtttt tagaccctgt tgaagcagct
    23641 aaattctgat catagaattt gagcaagagt cccattacca ctaaccccaa accataaagg
    23701 taaggagtca gtttccagat ccatttggaa ttcaaatgca tgatgacaac gattgctgcc
    23761 gctccaacta aataccagac aacttgcatc cctacgcctt tcaagactgc tggattgttc
    23821 ggatcatggc taagtgctac atataatgag agcagaccaa tcaaacataa cagaaagact
    23881 ggtaaaatca cgccataatc aatacgatta tcgttcgaag cttttaattt tttttccatg
    23941 tttcatccct tttcttaaaa accatactgc tgattattat agcggtccag ttagataatg
    24001 gaaagtcttt ccatagacat ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc
    24061 aaatgcagta caaaagccta ctgcttatct ttagtgtatc atagaacttt ttttgataca
    24121 tgcaattatc taccagctga gattctcctc gctgtgagaa gtttctgtgc tttcattgta
    24181 tcgaaggatc tcttcggcaa tctctagcag ctgatcagaa accagattgg ttcgttggat
    24241 gaaataaaaa tatcgatgga aattcaacgg agtcattggc acattttctg gaacagagcg
    24301 cttagacaaa atcgagcgac cgattccttc cttcagcaac gcaacaatta catcgttact
    24361 tttcacgatc atcttcttat cttggatatt catctcttca aaataacgct gcgtgtaatg
    24421 aaaaacacca gagtttgctt ctcttacaag ccacaatttg ctttttgggt ccccagcgat
    24481 gactagctgg tcttccatca aagagtatct ttgtacgccg gaagtctcta atggcttttc
    24541 gataaaacca aaatcggatt catgtttcgc tacttgttct aatgattcat gagaattcgt
    24601 gatttcgatc tcaaaatgca ctttaggaaa ccttttgatc agatgtttca tcaactgagg
    24661 caaaagatag acagcaaagg tgtgggaagc aacgatccga catacttctt cttcattttg
    24721 ttcattgagc aatttttgct gagtttcttc ccattcttct aacaaaacca gtgtccgttc
    24781 atataataag tcggcttgct tagttggaag catctctttt cttccgttac gttgaaaaag
    24841 atcgatattc aattcccgct ccaactgttt gatttgattc gagactgctg gttgagataa
    24901 aaacagtact tctgctgctc tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt
    24961 taataactta aacatggcac cacttccata actttttatg ataattacta taaaaactat
    25021 ttgatttaaa tatacaaaaa accatctata atagaaatgt acaacaaaac agttgctgat
    25081 ccaactagta acttagatga agacaatcag ctgtctgctt gctcgtaatc atacattcct
    25141 attcatttta caatgtattc gttcatcttg gtactaaaat ctattaaata aaaaggagaa
    25201 tattatatat gaagaacttt ttgaaaatta ttccgatccc gatctgcggg ttgatccttg
    25261 ggttagcttc ccttggaaat ctgtttaaag actatcacca tgttgcttta ggcaatctaa
    25321 ctggcggtat ctctatgatt cttatgatat tcatttttgt taaattgttt atcttattcg
    25381 aacacacgaa acaaacgttg catgatccaa tcatcgcctc cgtttcacca acttttacga
    25441 tgtcactcat ggttatttgt acttattttg tttcatatga aagtattgca ccaattgtca
    25501 aattcatttg gctgtgcgct gttatcttcc aagtgatcct tgtcttttat ttcaattatc
    25561 accatgttat caaagctgat ctttcaattg aagcaattta tccaagctgg tttatcattt
    25621 acgttggatt tggggtcatc actgtaactg ccgggaactt cttcccactt attggaaaaa
    25681 ttttcttttg gatttcattg atctgctatg cgttcttatt gccgatcatc atcaaacgtg
    25741 tctttttcgt aagaaatatg gctcatccga cacgtccgct gattacgatc gttgcagcac
    25801 ctggttctct ttgcctaaca ggttatctta aaaactttgc tgaacctaat ttttatcttg
    25861 tcgtagcatt gttcgcttta tctcaagtat tgtatttcgt tgtgctgtct atgcttccac
    25921 gtttgttcaa gcttcgtttt tatccaagct atgcagcctt tacattcccg cttgttatct
    25981 ctgcaacagc tatatttact actgttcatt acttcaacag tttaggtatc tatagtagcg
    26041 tattggattt cttaaaagtt gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc
    26101 ttgctcatta cgtacgtttc ttaattaaag aacatagcaa acatggaaaa tttgtttcta
    26161 aaaaaatcgc ttaataatcg attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat
    26221 aagaacggcc aaacaaaaac agctgttcat gtttttgtac tggttgggct tattaccgca
    26281 gatgcaagct tttgaacacc gtttaccaag aggtcgtaaa agaagtggtc agcttcaaga
    26341 aataagaagg aatttacgaa aattgcttct caaatttttg tgaaatttcg gcttatttcc
    26401 gtagaagcta cttctggaac accgtttatt cgaaacaaga ggtcgtacaa gacttttgtc
    26461 acgacctctt gttcttttgt caaaatactg gtttagtgct actatgttcg agtatgcttt
    26521 tagtatttat tgacgaaagg aaccgatcaa atgaaatcat tgtcacatcc aataaaatct
    26581 gtaaaggatt cagcggtccg cgaaatctta cccggtttac tgctttcttt tgccatcgct
    26641 gccttcagca ggcttcttgc tatttatctg ccacagttag gtggagcgac tattgctatt
    26701 ttattgggta ttttattagg aaatactttt tttaaacagc ctgttctagc aatcggcaca
    26761 aaatttgctg aaagcaaact attagaattc tctgtttttt tactaggtgc aactgtcaca
    26821 gctcaaacga ttgctcaaat tggtgtaaaa ggaattgttt ttgtgttact gcaaatgtca
    26881 gtcactatca tcggtgctta cttgattggt aaaaaactgc aattcagcga tacttcttct
    26941 ttgatgatgg ctggaggaaa cgctgtttgt ggttcttctg ctattgcttc aatcgcccct
    27001 tccatccatg cgaaagagga agaaaaagga cagatcatca cactagtcaa tctactgggt
    27061 actgtgatga tgttgactct cccttttctt ggattacagt tgtttggtgg aaatctcatt
    27121 gaaaaaagtg cacttttggg tggtacactc caatctgttg gtcaagtagt cgctggggca
    27181 agcttacttg acaattctgt tgtcgaattc gccatgctat ttaagattct acgtattatg
    27241 atgctcgttg ttgttgtcta tctgtttgaa aaattcatgc acaagaaaca accagctctg
    27301 tttgaaacgc caactgatac gcagaaaaaa cgaaaattac cgcttccttg gtatgtatta
    27361 gggtttgtga tcttttgttt ggtcaacagc ttgttttctt tacctgtagt tattgaccaa
    27421 tcggcacatt ttatcagcag ctggtttgag atcactgctt tagcggcaat tggtctgcgt
    27481 ttagactttg caaaattcat gaaagaaggt acacgcttct tatcttatgg attagctgtc
    27541 gggcttctcc aaacagtgat ggctttacta tttatttggc tgatcaaagt ttagaatgaa
    27601 catgaaaaaa cagttgtgat tctcgcatgg aacaatgtcc gaatcacaac tgtttttaat
    27661 tttgcagata tccttctaac aacaacaatt ggcgtttgat ttcaacccca tctattgcgt
    27721 ttcccatata tcctgtcagt tttccatttt gcccgatcac tcgatgacaa ggaataacga
    27781 ttggcaatgg attggttcga tttgcctgcc caatcgctcg cacagctttc ggactatcaa
    27841 cagcaacggc tacttcttta tacgttcgga tttctccata aggaatggtc gacaaagctt
    27901 tccagacatt ttgctgaaaa ggcgtacctc tctttataga taaaggaaca tcaaattctt
    27961 ttctttttcc cgcaaaatat tcttcaaact gttcttgtgt gcgcgttaaa attcgctgat
    28021 tcgctgattc gctttctgaa attggctgaa aagatacagc agtcaatccg gattcatctg
    28081 catctaacca taaaaagccg ataggtgtgt gtattttcat tttgtttgct cctagtcaat
    28141 agactggaac ttcctccttt ttctctatcc aaaactactt gccaactaat aaaaattgtt
    28201 cttaaaagtt tgataaaccg aggaaacatg gtaaactaaa tacgttagtt tacaaaaaga
    28261 atacctgttc ttttacgtaa aatcaaattt taaaggtgtt atttaaggtt tattaaattt
    28321 tgaatgtaaa ccatatataa acatacgatt gaaaggatag aaacgatgaa agtatcaatt
    28381 gttgtcccat gttttaatga agaaaaaact gttcctcttt tttttgatga ggttgaaaaa
    28441 ttcaaaggtg cccatgagtt cgaatatttg tttattgacg acggatcaaa agacggtacg
    28501 ttagcagcaa tcaagaagct agcctcagct tatccttatg tccgctttgt ctcattttcc
    28561 agaaattttg ggaaagaagc ggctctgtac gctggattgc aagcttctac aggagattta
    28621 gtgaccgtga tggatgttga tctgcaagat ccgcccgaac tgcttcctga gatgatcaac
    28681 cggatcacta cttctgatat cgattgtgtc ggcacccggc gttcgactcg tgaaggcgag
    28741 cctgcgatac gcagtttctt tgcgaaaaaa ttctaccagt tgatcaataa aatcagcgat
    28801 acagaaatgg tcgatggcgc tcgtgatttc cgcttgatga ctcgtcaaat ggtggatgcc
    28861 gtcctagaat taacagaata caaccgattt tcaaaaggat tatttagctg ggtcggattt
    28921 aaaacagaat atatttctta tgaaaaccgc gaacgtgtgg ctggcgaaac atcttggtcc
    28981 ttctggaaat tattcaacta ctcgattgat ggtatcgtga acttctcgga tgcaccgtta
    29041 acgattgctt catttatcgg cgctttgtct tgtgtcggtt ctggtattgc aattttgttc
    29101 attattttgc gggcattgtt gtttggtgat cctacatcag gatggccttc catggtctcg
    29161 atcttcctgt tcattggtgg aatccaattg ttatgtcttg gaatcatcgg gaaatatatt
    29221 gggaaaatct tcttagaaac gaaacagcgt cctgtttata tcataagaga aacagaaaaa
    29281 gtgaagccaa atgaattgaa gcctcaccaa taatatacgt caaatgaagt aaacattttt
    29341 ttaaatgaaa aaggactgtg acaaaagtct tgtcacagtc cttttttccg aataaatggt
    29401 gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaagcaatt
    29461 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct atcacaacct ctttcatatc
    29521 ttattacttc ttcattgctg cttttagtgc tgctgctaat gggctttctt ctggttcttc
    29581 tgcttgtgca tattttttca gcaagcgtcg ttcttcatgt ttggtcattt tcttcttctg
    29641 ctctttttta tcaagcaatt tttctgtcgt tccatcgtat ttacaacgga aataagcacc
    29701 gtttttacct tcgatgattt ccattttccg gtgacattgc gggcatcgat gattagagac
    29761 ttttggatct tttcttctgc ggtagttgca atctgaattg gtgcagacat agattttgcc
    29821 gtcacgtgtg tttttctcgc gcagattggc cccacattct gggcatttct tttgggtaat
    29881 cgagaaatct tgatattctt gtttgcttga tttgatctca ttcaccaagc gttttgtttc
    29941 tttttcgatt ccattcaaga aggttcctgc ttgtttcttt cctgcagcaa tagcttcaag
    30001 gtctttttcc cagctttctg tcaaatctgg tgttactaaa gagggattta ccaatactaa
    30061 taattgctta cctttaggag atacttgcag tccttgaggg gtacgttcca tcagttcaga
    30121 tttgattagt ttctcgataa tctctgcacg cgtagctggg gttcccagac tgtatttttc
    30181 catttgacca agcaagcttg cttctgacaa tggtttaggc ggtgcagata gttctttttt
    30241 gatggcgaaa tcgggattga ttctcattcc tttttcccat ttgactgttt ccgtctcagt
    30301 ttcagtttcg cctttccatc ctgcttggac gacacgattt tgccgaaaaa tgaatcgttc
    30361 tttaccaaat tcaaccgtaa cttttgtttg gctgacttta tggggttgag caaacagtcc
    30421 taaaaaacga gagacaatca tttgatagat ttttcgctct tcattactta atttttcata
    30481 acgtgctctg ttttcagttg gcaaaagtgc atggtgatcg gtcactttac tgtcttggaa
    30541 tacttttgtc tgttggacga tggctccgtt tttcaaatac tgcttgacct caggagcaaa
    30601 gtcgctgact gcttgcaatc gttctttcat cgtacttttc atgtcatttg tcagatattt
    30661 gctgtctgta cgcggataag taacgatctt atgggtctca tacaaacttt gtacaagact
    30721 taatgttttc tttgcagaat agccatagcg ttgattggct tctctttgga tctctgttaa
    30781 atcataaggc aatggcgcag attccgtttt taatttttct tggacttctg tgacagttcc
    30841 tttgcttgta ctgatcaatt gaacaagttt ttgtgcttct tcttgtgttt ttaaagcaaa
    30901 ttgatttttt tgaagcattt tggcttgttc ttttccagct tgcaacgtga tcgtgaaata
    30961 cgtttgtgga cggaactgct cgattttctt ttcttggtct ctcactaatg ctagtgtcgg
    31021 tgtctgcaca cggcctgctg ataaattatc cgcgtacttc acagttaagg ctctggtcac
    31081 attcaatcct accagccagt ccgcttttgc ccgggcaaga gcagattcat acaatgcatc
    31141 atattctttc gcaggtctta gttgcttgaa tccagttttg attgctttat ctgtttgaga
    31201 agagatccat aaccgcttca ctggtttatt gaaatgcaca tactctaaga tccagcgagc
    31261 aacaagttcc ccttctcttc cggcatctgt cgcaatgact gcttctgaga catcttttcg
    31321 ctgagccagc tgcttgattg cttttaattg atgtcctgtt ttaggaagtg gtttgattcc
    31381 tagtttttta gggatcattg gcaacgtgtc catttgccag ctctgccatt ccttattcag
    31441 atcttccggc attttcaatc ctaataaatg accaagggcc caagtaacga tcaccttggg
    31501 tccttcataa taatttttgt gcttttgatt agcaccaagc accttgctta ggtctttcgc
    31561 aacgcttggt ttttccgcta gaattagttg tttcataaaa ttcctttctg ctttctactg
    31621 tgatgggctg atgatttttc aacaacgcaa gtcccttgta ttgctgtaaa tcatcgtcac
    31681 actcttcaca ccagcgctca tctccgtcat tccaaaaggc gctgagaaag ttactttttg
    31741 cattgatata ttcataattt tgatggattt cgttccattg tttttcgtac gcttgtcgtg
    31801 cttcttcgaa agactcgaat gtttcttctt cttcaatatc ttcttcccag tcttcaaaaa
    31861 accaccaagg ctcattgtcc ccgaacatgg taattacttg atacatactc gtgctccttc
    31921 tttcagcttt tctagactca tcttagtcac ttttcaagaa agttgcaaga attttctctt
    31981 ataaaagggt catttaatga acatcttaaa attgttacaa atgaatctga ttgtttaagg
    32041 caaagtagcg ataaagttat tgatcatcca aaaatagctg tcttctggat cgattgttgg
    32101 ttcttttgta aagtatccta aaaaacgtaa ggaaagataa ccgtggatca tactacgaag
    32161 ttccctgctt cgatgaactt tttcttcttt cgttaattga aaagcctcaa atatctgcaa
    32221 aatgatctga ttcgtttcat ggattccatc taataaaaca gcttcattgg tatgcggtac
    32281 actgatcagt aactcataca ctgcttgatt tgaaaaagca aaggatcgat aagtttctgc
    32341 atagatctgc agtgcttcct gaccactttt tcccacaaga gcccgtcgca gctggtcatt
    32401 aagttcctgc agaagtttcg ctgttagcgc tgttttgaca tcacgaatat ttttgaaatg
    32461 gttatacagc gagggagcct tgatatccaa gcgtttggct aaatgctgaa aggttatttg
    32521 ctgaacatcc atctcttctg ctagttctcg aaagcagtcg atgattttct cttgagacag
    32581 atttcttttt ttcatttcca tgtttcctct cataaaataa atccttcact ctttcagttt
    32641 accacgaaaa agaaagagaa aaaagagact ttcattgata aactacatat tgtagtttat
    32701 aataaagagc tgtagatgaa aattttcaga aagaaggagt ctaattggct gcaagaataa
    32761 aggaaaaatt atttaggaaa agagaaactt gggaacaaaa cttgtttgtc ttatggtttg
    32821 gaacatttat ggctggtatc ggatttagtt tagtcatgcc ttttatgtca ctttacatag
    32881 atacgttagg taattatagt acgtcacaat tgaacttttg gagcggattg actttttcat
    32941 ctactttctt ggtgactacg cttatctctc cttggtgggg aaaattagcc gaccaaaaag
    33001 gaagaaaatt gatgctgttg cgtgcttcat taggaatggc aatcgtcatt tctttgatgg
    33061 gttgcgtgac tagtgtgtat caattagtcg cattgcgtct gcttcaaggg attttttctg
    33121 gttatatcag taatgccact gcccttgtcg caactgggac gccaaaagaa aaaagtggtc
    33181 aagttctttg aaccttagca actggttctg ttacaggaac attacttgga ccgcttcttg
    33241 gcgggatctc tgcttcagcg tttggctatc gccctacctt tttcattaca gggatcatct
    33301 tatttttcgt cttcttgttg agtctgtttt tcgtccatga aaaatttgtc ccagtgaaaa
    33361 aagaaaacat ggtttctgcc aaacagattt tcaaagactt gaaatatcca cacgtcgtca
    33421 tcggtatgtt cgtcacaacg atgatcatcc aagcatccaa caattccatt agtccaatca
    33481 tcagtctgta cattcgccaa ttgctgcatg gacatgggaa tgttacgttg atcagcggaa
    33541 tcattgcttc gatacctggg attgccactt tgatagccgc tccaagattt ggtcgcttag
    33601 gtgacaagat tgggagtgaa agaatacttg cgattggttt gggttttgcg attctggttt
    33661 atatcccaat ggcattcgtc acgaatgttt ggcaattagc tgccttacgt tttttgattg
    33721 gtatctctga tgcttgtctg ctccctgctg tccaagcatt gatcacaaaa tattcgcctc
    33781 atcaagctgc tggacggatc ttcagttata accaatcttt ccaagcaact ggtaacgtag
    33841 tagggcctat gatcggctcc agtgtttcca gtgcatttgg ttatcgcggc gtctttcttt
    33901 cgacttctct tcttgttttg gtcaacttct tgcttgtacg acacaataca caagagatcc
    33961 atcagcaaga aactgcagaa gaaaaagctt caggagtctc catagcttca gggcactctc
    34021 cttctcatag acaataagcg taaatgaaaa acactgcttg aacagttgtc acagactgtc
    34081 cttgcagtgt ttttgtttag gcctgttttt tcaagaactc agataagagg aataatccct
    34141 tttccaaggt ttctttttta caacagtaac ctagtcgggc atgcttcggt aaatcaaagg
    34201 cacttcctgg tactaatagt actcctgttt cttctaataa attgatacaa aatgtctggt
    34261 catcttcttc gatggctaat ttaatgaaag aagtagagac gtaattggga gcgacaagtt
    34321 caactttcgg ttcattggct acccattgtt ttaaaatcac aaggttctct gtaatgatct
    34381 ttttgtttcg cgccaatatt ttttctttat tcttcaatgc atgtacagca agatcatctg
    34441 acaggactcc gccgcaaatc atcgtgtagt ctcgatactt tcgaaaaact tctgccaact
    34501 caggtccagt agctgtccag ccaatacgaa tacctggaat ggagtaggtt ttggataggg
    34561 aattggttgc tatacctttt tcatagacat ctacgatcga caagaattca cctttatccg
    34621 ttaaaggcgc atatacttca tctatcaata catatgcatc tactgtacga gcaatttctg
    34681 cgattttttc taaagttttc cgatctagca atgtgcctgt aggattgttt gcactattca
    34741 aacagatgat ttttgtttca ggtttgacta aggcttctag ctgttctaaa tcaaactgcc
    34801 aatcttcttc ttccttcaaa tgaacaaagt caacagcggc acctaaagat ttggggatgt
    34861 catataattg ctgataagta ggaagcatcg aaacaacatg gtctcctgct tccactaacg
    34921 cgtatagagc taaaagatta gcaccagtcg ctccattcgt ttgtaagatg ttttccggat
    34981 cgacggtttg ataaagagaa gcgacttctt gtttgaaaac atctgatcct tcgatccagc
    35041 catagtccaa tggttcgttt ttgcgttttt cgaaaaattt acttacgcta gtcccatcta
    35101 agccaacgag ttcgtctaat gtcaatgctt cgatcgagct ttgtgcaata tcatataccg
    35161 ctttcttttc ccatttattt agccattctt ctacaccgaa atttgcgatt ttcatgtttc
    35221 tttccccctt atatcttgat ccagcctaca gcaagcaacc cgattgaaag cagtgcggcc
    35281 acacaaatca gcccaatcat tactttttct tttcccgtaa tttgatgttt ggtttcacga
    35341 caagcgatcc agtagaagca aaaaccagga agataggcaa tactacataa aaggacataa
    35401 gaaaatcctg ctaaaatgat gcccatcaat tcaaaaacta cagcaatccc accgatcact
    35461 acttgttttt tgattccttc ttttaaagaa aactggactt gatagatccc gacaagtaga
    35521 taacaaatca aaattgccgc tgtacataaa gaataggcaa attgataggc ataatcagta
    35581 actaagaaag tcaataagaa tagattagtc aagattgcag taacaactaa cgaataagtt
    35641 ggggcatttt ttttattcgt ttttccccaa cctttcggca acagaccgtc tcgagccatc
    35701 aacatcgttg tttctgctgg gagcatcgtc caagacaacc aagaaccaat gatcgaaata
    35761 atcaagccga tattgatcag cgctgctccc caatttccca cgattccttt taaaatattt
    35821 gccatagcag gttgtgaaat agttgctaac tcttcttgag ataagacgcc gtatggcaag
    35881 atcgaagcaa ggatatagat aaacaataaa caaagcagac caatgatcga tgcttgctgg
    35941 gcttctgaac gtttcttcgc gcggttggct aaaacacttg cgccttcgat ccctacaaag
    36001 acccacatca taaccatcaa acaaccttta atctgcaacc agatcgttcc ttgctgccct
    36061 gttccattca ctagattatc agatacgtta ccccaaaaat cagcagtgaa tatattcact
    36121 ttaaatgcga taaatacaaa tattaaaaat aggaataatg gcactaattt acagatcgtc
    36181 acgattgtat tgatgacgct cgctccttca actccattgt ttactaataa agtcagaccc
    36241 caaataaaaa tacttgcaaa taaaatagaa ggaacatttt gtccaccttt gaatatcggg
    36301 aaaaattctc ccatggcgct catcagcatc gttgcaaaag caacatttcc cagccacgca
    36361 gacaaccagt aaccccaacc actgatgaag ccacctaatt taccaaatga agcttccgcg
    36421 taaccgaaaa taccaccatc tagatctgga cgcttttctc ctaaatgatt cagcgataat
    36481 accaaagcta aaataccaat cccaacgatt atccatgaaa ggatcgctgg ccctggagct
    36541 gcagaactag ccagatcact tgtgatccca aacacaccgc ttccgattga cgaactgaca
    36601 accagagcaa ccagtcccca tttgctaata cccgtcgatt tcttttccat ttccatcacc
    36661 ctcacttata aaattgaaca aatcacttgc aaaagattcc cttctatttt gcaactgctc
    36721 ttgcttaaca aaaagcaact ttattcatat tattggatat tatgaacttt ttacacaagt
    36781 atttcaagta taaaatgaat aaaaattaat tttattgcaa aaaaacagct atgctatcca
    36841 aaagaaaagg tgtttctttt gttagccata gctgttttga ttcatttaat atcgcttaaa
    36901 cgtattgtaa aacttttgtt tcttgatatg cgtggtcgat caaaccacca cccagacatt
    36961 ccattccatc ataaaataca actgcttgtc caggagtgat tgcacgtaca ggttcgtcga
    37021 atacgacttc tgcacgatta tcatccaata agcgaactgt cacaggaacg tcttgctgac
    37081 gataacggaa tttcgctgta cacttgaatt cttttggcat tggttcattc gtcgtaaagt
    37141 ggatttcact tgcatccaag cttgtggcat acaatgcagg atgatggaac ccttgcccca
    37201 catataatgt gtttgtcgct agatcttttc ctacaacaaa ccagggttct tgcgaatctc
    37261 ctccgccacc gatccctagt ccttgacgtt gaccaatcgt ataatacatc aagccagcat
    37321 gttgtccttt gacttcacca tcaagagtga ccatatttcc tttttttgct ggcagatagt
    37381 tgctcaaaaa ttgtttgaag ttcttttcac caataaagca gattcccgtt gaatcttttt
    37441 tcttagcagt cgcaaggcct gcgcgttcag caattgcccg tacttctgat ttttccatgc
    37501 cgcctagagg gaacatcgtt tttgaaagct gttcttgtga taattggcta aggaagtaag
    37561 tttgatcttt attattgtct actccacgaa gcatgtggac cacgccgttt tcatcgcgag
    37621 tgacttgcgc ataatgtcca gtagctacat aatcggcgcc aagatccatt gcataatcca
    37681 agaatgcttt gaatttgatt tctttgttgc acatcacgtc tgggttaggt gtacgtcctg
    37741 cgcggtattc ggctaggaaa tattcaaata cacgatccca atattctttt tcaaaattca
    37801 cagagtaata aggaatcccg atttgagcag ctactttcgc tacatcctta taatcttccg
    37861 tggccgtgca tacaccgttt tcgtccgtgt catcccaatt tttcatgaat attccgatca
    37921 cgtcgtatcc ttgttccttt aacaaaagtg ctgttacaga tgagtcgaca ccgccgctca
    37981 tccctacgac aacgcgtgtt ttgctgttgt ctgtcatgat atcaccatca tttcaattag
    38041 attctgaaac atctacgagt tgaaggtcgc aacttttcag tattttacat tatacgtcat
    38101 atttctgaaa atttcatccc ttaattatca agtcgtaaaa atactgttct actggaccta
    38161 ttcagtacac aaacatgata gtcaaccaaa aaatcactca tcttttgagc attcgatata
    38221 aaaaattggc atactcataa aaagggccag cctttcggca ataaggaata attttataaa
    38281 ttgtgaaaaa ctatttttca aaattgcttc ttatcgctta aagctgcacg tctcatttac
    38341 aaccttttct ttttagatgt tatgagttgc tcagataagt gattttgttt actctaagaa
    38401 aaaagggagg caaaagatac atgaattact ttatcagtga tatgcacttt ttccatgaac
    38461 gcttattagg aaaaaacgac tttgctccgc ggctctttgc aaatgtggat gaaatgaacc
    38521 aacagttgat cactagctgg aatgcagtaa taaaagaaac cgaccatgtt tatcatctag
    38581 gcgaccttgc tatgcatccg caatatgaaa aagcaaatgc agaaattttg gaactggtca
    38641 ctcaactgca aggaacaatc cacttgatca aagggaacca tgacagtcgc gcttttttcc
    38701 gttatttaga aaaaaatgat cctaggctta ctgacggaag tcccaaattt tatttttatg
    38761 atgtaggtgc catcgttaaa ttcgaccatc aacaatatta cttgactcat tatccgatgt
    38821 tgctcgggcc taacgacact atccgcaatc ttcatggaca tatccatcat tacagcgtac
    38881 ctatcgagaa cgatgtcaat gtaggcgtag acgcaccgga aagagaactc ttaaaggaaa
    38941 aactaccatt tggcactccg ttatctgagc aacaaatcaa agaaatctat gaagcaaaga
    39001 ggaagtacga taggtcgtga aaaaactgcc ctatatcaag tattaaacaa ataaagataa
    39061 ttactcatat ttttcatttg agtaggctta ttgccacagg aacattagga tcagaaatgg
    39121 agttgtaaca agacttctgt cacaactcca ttccatacta ttcgtctttt tgctcatggt
    39181 gttcttttac cagttttttc tgtaattctt tatcataatt tcgggcagct gacgttaaaa
    39241 ataaaacgat tccgcataat aaaatcccga ttccggaaaa caagaacatt ttttctaccc
    39301 ctaaacgatc agctaatggt cctgcaaata ataatccaat tggacctgcc aggttcatca
    39361 aagagttgaa gttccctaag acacgaccta aaacattcga ttcataactc tgctggatca
    39421 ttgccatcag cagtgtattg aaatatggcg ttgcacaacc tgctcctgca ttcaagataa
    39481 gaaagtacaa gaacccttga ctatcgtttg gaaccaatcc gcttgcaccg atcgtcaggc
    39541 ccataatgat gtaggctata ataataggtt tcattcggtc tttccatgtt cctataaaac
    39601 tgatcagtgc accgcctaag agcattccga ctgcgtagac tacttccact gctcccgcct
    39661 gtccaacgga accaccaaag taatctagcg tcatcaatgg atacagactg attgctggca
    39721 tgaacaacag catcacaaat gcaccattga tcgttatgta ccataaccct ttattatcca
    39781 tcaattgctg cagcccaaat tttgcatctt taaaaatagt aagcggaact tccaatattt
    39841 caggagaaac ttttgggatc ttcacaaaaa tcaataagcc gacaccaaaa actgccccca
    39901 atacatctaa taaaataagg taattgacag gaacgactga aaacaaagcc gcacctagag
    39961 ctggcgcaat gatgaaattc gctgattgga ccattcctaa ctgtccattc gtccttgtta
    40021 tgtgagaaga tggaacgatt gtcggcataa tcgactggat cgttggcatt tggaatgttt
    40081 gtgcgaccga acgcataaac aaagacacaa aaactagcca aatcgggaag gattctgaaa
    40141 tcgttccgac tactgctaaa ataagcgcga aaatcgctac aataatatcc gtgacaatca
    40201 aaagagcttt tttatcccag cgatccacta gcggtcctac aaatgggctt agtaaaacca
    40261 tgggaatcat tcctagtaaa gtagcaaaac tcaaaattgt cgccgaaccg gtttctcttg
    40321 tcaaatacca gatgatcgca tattggacga ccatactagt gattcctgac aaaaactgtc
    40381 ccgataaaaa gaacaaataa tttctcttcc aattcgttaa aacgaccgac tgttcatttt
    40441 ccataaacat ccctcttttc tttttcattt tgtatagcgt ttgcacaaca atcattatac
    40501 gggattcttt tttcaaaaaa tacctttttc cctaaataac aatcgtttca atttaaattt
    40561 ctttacttag aaacaataaa tcagcaaaat attaaaaata acaacatgtg ttaaatttaa
    40621 aaataaaaaa gacaactaat ttgcgtcgtc cttacaaagt tagtcatcta acaataggcc
    40681 gtttcaaaac tagttgtctt tttatatcca cagatcattc gactttttcg aataacccac
    40741 ggctaatcat cccatccatc agaaaataaa atttatcttg gctcattttc tgttttggat
    40801 ctttgaaaat attgactgct ttcaatccat ttgctgtagt gatcgacatt ttcaatgttt
    40861 gaatatcttt tgatacagtg atttttaatt tgaatccttc tttgctttgc ggcgtggcat
    40921 ctaatggtcg gatcaattga taattgcctc cgtttgtttc tgtaaatcca ttatcgcgca
    40981 atgtatatct ttttgcatca ggatgcaaac gatagctaat tggtgcgccc agtaccgttg
    41041 ctgttgtttc aaatgccatg attttcacct cgataaaatt agtttctctt tattataaaa
    41101 gtttatcctg atttttacta ggtttttcag ttaagttccc taaaaaatga aggcgttctc
    41161 tcagacatct gttcatataa gaatgacttc actagttatt ttttgacagc acgaactaat
    41221 tgatcaagga aatactggat gtcgtcttcg gtatttccca aaccaaagct gatacggatg
    41281 gattctttta gtatcggtgt atcttctcca tacattgctt ctaatacgtg tgaaggctcg
    41341 atatttcctg ctgtacaagc tgaaccagta gaaatagcga ccccattaag atcaagcttc
    41401 atcagtagca aatcatttgc ttcgccaatt aagcgaagat tcaacacatg agggagttta
    41461 ttcgttaaat caccatttac ttgataaggg atttcgtttg cttctaatgt atcaagaatc
    41521 aattgctgga atcctttata tttgttgtta cgtgcttcac gttcttcagg tgacagcagt
    41581 tcaactgctt ttgccatacc ccagatccct gctaaatttt ctgtacctgc acgccgcttt
    41641 tcttcctgct ccccgcctaa aagcaatgca gggatattta ctccgtctcg tttatataag
    41701 aaaccgacgc cttttggccc attgatttta tgagcagaga tacttaataa atcgattcct
    41761 aattcttctg gatggatcgt ttggtttccg tacgcttgta ccgcatctgt atggaaataa
    41821 gctggatgat ccgctaaaag ttcaccgatt tctttgattg gcatcaaatt tccgatttca
    41881 ttgttcccgt acatcacaga gaccaagatc gtatcttctc gtaacgcttt tttgacctct
    41941 tcgatcgaca gattcccttt ttcatctaca ggcagatatg taacgtcaaa cccctttgtc
    42001 tctaaatatt tcatggtatt aagaacagca ggatgttcga tagctgtagt gattagatgt
    42061 ctgccttctt tttgacgcga gaacgctgtt tctaagatag ctgtattatc cccttctgtt
    42121 cccccactgt taaagatgat ctcgtgaggt tttgcttgca gactgtctgc aatgatttgt
    42181 cttgctgttt ccaatttttc atgcgcgtgg cgaccaaatc catgtatgct tgaaggattt
    42241 ccaaacgtgt cttgcatgat tgccgtcatc gcttcaatta cttgtggatg catgggggta
    42301 gttgccgcat gatccagata aatagttttc acttcattca tcccttccaa attattgcta
    42361 ggcacattct agcataaaat agcgaacaaa ccaaagaaga gctcattttt gtctagagga
    42421 cgtttcatgg gcatagaaaa aaggagctgt gacaaaagtc ttgtcgcagc tccttgaagc
    42481 tgctcacttc tgtcacaact tctttccctc ttttttttgt ttattcgaat tctttttctt
    42541 ccagacggaa taacggactc atcggtgtat tttcatggat gcgtttgacc gcttcaccca
    42601 ttaattctga acatgtaacg attgttaagt tttctggatg acgattttct tctgtgaaaa
    42661 cagaatcagt gatacaaata tctttgattg gtgcattgtc taaaatactt ttcgcatgat
    42721 cggacaataa tccatgtgac gcacaggcat atacttcttt tgcgccgttt tcttttaata
    42781 catttgctgc tcgtgcaagc gttgctcctg tgttcaagat atcatctacc atgatacatg
    42841 tctttccttg aacatcaccg atcacataac caccctcagt tgaagcatca tctgcatgat
    42901 ctacgatggc taatgtgctg ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc
    42961 tgttttttgg cgaaacaact acgatgtcat ctcccgtcag acctaattga cggtaataat
    43021 gagcaaacaa cggcatcgta aataaattat ctacaggaat atcaaagaat ccttgtactt
    43081 gaaccgtgtg cagatctaaa gtcagtactc gagttgcccc tgcttcttcg atcaagttag
    43141 cgatcaattt cgcagtgatc ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc
    43201 catagtaagg caatacgaca ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca
    43261 tgatcagtag ttccatatag taatcattga caggtgcatt cgttgattga acgatataca
    43321 catgatcccc tcgaatactt tcttcgatat tgatggaaat ctcgccatca ctaaattgct
    43381 tcacgacgct tttacctaat tctgttccga acactttcgc gattttttca gctaatggac
    43441 ggttgccatt caaactaaaa atcttcaacg tgtcatcctg atacttatta ctcatgtagt
    43501 cctccccaaa acttctctat tcttcgatag tttaacatat ttttccaaga ttttctgtta
    43561 atttcagcaa attcttaagg aagtgaaact gttccgttac tccaactggc aggaatgact
    43621 ttatgataat cgccccagtt gtcgtgtact ttgtaataac gttctcctgt ttcagtttca
    43681 aaataagcgt acgctgtcac ccaatgattg ccatatgtac tgccaagtac tttcaaaata
    43741 ccaatcatca caggtttgcc ttcacgtata cgttttgtag ctcgctgcca tgaacctaga
    43801 agagttgctc gtgcacgctc atgattgcca ttcttcttta aaaaactaga aatccctgta
    43861 ctaacctgaa aaggaatcgt aggaagtcct agtggttgga tctggctacg caatgccttc
    43921 gtcaaaacca acgaatcagc cgtatttttc ttgcgaatct catttggaag catccacccc
    43981 ttacgatagt cttgataata ggcaaggaga actgctgccg cgtaagtacc acaaagatag
    44041 cctgaggggg tagcccattt cttatacctc aaaaaacgtt cttctggcaa tcccacccag
    44101 caatttgcga gttcctttcc tgaccttttt tcttcgatca aaacgggaat atcttctttt
    44161 ctgttgatcc atctagataa ctcgattggc tcttcattga tcagcttcca ttttttacaa
    44221 gaaagaccct cttgataagt atattttgct ggagaatatt ctaccggatg tcctgattga
    44281 tcgataatca cataactttg atcttcaaaa accagagcta aaaaaagcaa tttacttttt
    44341 cttgactgca gctgctgagt gctggctaat tttttaattt ttttatctac tttgaaatat
    44401 aaaggtttat ccactacttg attcagttgg gatcgcctcc tttttttcat tataagtgaa
    44461 ggattgaaaa aaacaaatca gtttgattgt tcgaattttc aattttttat gttctatttg
    44521 ttaattcctt cattttgtaa gataatgggc acacgaaaca aaaaaggagc gatcgatgaa
    44581 tgaacaaaga acgattgaaa caagatttct atgaagcagt caatgaagaa tggcaaaaaa
    44641 cagcaaaaat ccctgcagat aaaccagcta caggcggttt ccaagattta gttgacggga
    44701 tcgaccaact tttgatggga gaaacagacg atctgcttgc ccatccagaa aaagtgtctt
    44761 cagaaaaaat gaaacatttc cttgcatatt atcaactggc aaacgattat gaccgaagaa
    44821 atgagttgca tgctcaaccg cttcaaccta taatcgaaaa agttgaatca ttgcagtctt
    44881 tcgacgaatt gaataagcaa tttccagaat ggctgttgga tacattacca ttgccattta
    44941 atctggatgt agatgcagat atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc
    45001 cttctttatt ccttccagat aagacgtatt atgcatcaga ccatcccaac ggtgcacaac
    45061 taataggcgt tttctttgat atggctgttc agttattaga gcttgccggc aaagaaaaaa
    45121 gtcaggcaga agcaatcacg gaacaagcag tgcaatttga taaattgatt gctcctcatg
    45181 taaaaagtgc agaagaaaac gcagattaca gcaaaatgta taatccgcaa tcttttgata
    45241 catttattaa atacacagat aaactggact tgaaaaaact tgtcacgggt ttgattggga
    45301 caactccaga taaagtgatc gtaactgatc cagtgtactt cgatcatttg aatgacatcc
    45361 tgacagaagc tcattttccg ttattcaaaa gctggatgat cattaaaaca gtacgttctc
    45421 ttagtggtta tctatcagaa gaattccgcc aagtcagcgg aatctattct cgcgtattgt
    45481 ctggaacaga cgaagcaatg cctcagaaaa aagcagcgta ttaccttgcc tctggacaat
    45541 tcgatcaagt agttggcgat tactacggaa aaaaatattt tggcgaagct gctaaaaaag
    45601 acgtagaaca aatggtgaaa aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg
    45661 actggcttgg ggatgcaaca aaagaaaaag ccgtcataaa attaaataac ttaggtatcc
    45721 aagtaggcta ccccgataag atcccagcat tattcgacca gttcaaaaca gtacctgcag
    45781 atcagggtgg tactttgctt tcaaatactc ttgttttcag caaactcaca ttaaaagatc
    45841 gtttcagcaa atggaacaaa ccagtggatc gttcagaatg ggaaatgagt gctgacacgg
    45901 tcaatgctta ttatcatcca tttcggaatg tgatcgtctt tccggcagcg atccttcaag
    45961 caccatttta cagtctagaa caatcaagct ctgctaatta tggtggaatc ggagctgtta
    46021 tcgctcatga gatttcccat gcatttgata ataacggcgc tttatttgac gaatatggca
    46081 acttgaacaa ctggtggaca gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa
    46141 tgatcgaaga attcgatggt ctgccttttg ctgacggaaa agtaaacgga aaattaaccg
    46201 tttcggaaaa tattgctgat gcaggcggtc taagctgtgc attggaagca gcaaaaacgg
    46261 aatcagatct ttctatcgag gaattctttg tcaattgggc aacgatttgg cgtacaaaag
    46321 caaaaaaaga ataccaacaa cttttactgc aaatcgatgt ccatgcccct gctaaactcc
    46381 gagcaaacgt gcaaccaaaa aaccttgacg aattctatga aacatttggt atcacggaac
    46441 aagacgctat gtatctagcg ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag
    46501 aagtggtcag cttcaagaaa taagaaggaa tttgggaaaa tcgcttctca aatttttgtg
    46561 acatttcggc ttatttttcg aagaagttac ttctggaata ccatgtatta gaggttgtga
    46621 aaaagctgct tagtttcaag tagtaagaaa aattttgtga aaatggcttt ccacattttt
    46681 gcataaattt gacttactac cgagaaactg gcttttgaac accgtttatc gagaggttgt
    46741 gaaatcagcg tcctgacctg agtattagag aaaaaaacgg aaaaatagtt cctcatattt
    46801 tgccgtttgt tgagctaata ccgagggtca gctgattgaa caccgtttag tggagagatt
    46861 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaagcag caaggagaac
    46921 ctcatctata aaaagataga aggaagtgag acatctattg gtctcatttc cttctatttt
    46981 tactttcctc agtcaattgt tttacatcat aaaaggagaa attccactta tttttttcgc
    47041 tgtttcatga cataaacaac tacaccgatt aacaaaattc ctccagctaa tgctagcaac
    47101 gggttattaa attctcccgt ttttgggagt ccttgagtgc catttttttc actttctttt
    47161 gctgtcctat ccggatgaga aggttttcca tttggttttc catttggtga ttcatttcct
    47221 ggattttctc tttctggttt gtcatttccc gggggcacat cggcagtttc tcgcacagtg
    47281 atcgcttttt gctgggtaac agattctgta tgagcagacc atttttcccc atcaaatatt
    47341 tccgaacgat aagtgacttc tagcgtatat tctccttttg aaggtgctga aaaaatagcg
    47401 gtatgattag aaaattgagc acttgactgt gtttcaacga tttggatctt ttctgggaaa
    47461 taacgcatat cttcaaagac aggcgttcct tgatttggtg tgaaagttga cacaaaaact
    47521 gtaaaaggct tcagttcaat gatatttcct tctgcattct ccgccacacc atttgcttct
    47581 tgtataataa tcggattatt ttgttcattt ggttttgtct gatttggttc ttgtacgatt
    47641 gcatactctg gatattccga agacggtgag gtttctccgt caaaaatagc acctacagat
    47701 aatggaagac catccactct ttccttttct gtttgacgat tccccggttt aatctgcatc
    47761 agtccatgaa catggatact tccattttca tagcgcgtaa caggctgctg accttctttc
    47821 gaacatgtat ttagagactc gatccaatct tttgatatgg tatgtccaag agtattttct
    47881 gattgtttct ctcgttcatt ccaatagtag ttcaacgagt ggtagatttg atcttcgttt
    47941 tgattcaatg ggcggatctg ctctctcatc tcaagattct ctgttcggaa agataagata
    48001 ccagaagcat ggtagtctgt gtttcctgct gaagaggtat aaagagccac attatatgaa
    48061 ccgttattaa aacttgtact gctattgacg ataatatctg ttccgctatt ggaatcgatt
    48121 cctttcgcaa gattttcgaa agacaaacta ttgatcaatt catgagggcc cgttaaagaa
    48181 gaaccgccca ttttgaatcc attaccgtta ccttctccct caattcctgg tacccagccg
    48241 tttcgataag caactgagtt tttgatgatt acttttccga tcgaacctgt ttcattttta
    48301 gcaaaaaggt cccaaccgtc atctgcatta tgatacgcga tacaaccgtc aaatacattt
    48361 ccttctccta ttgtcagttt ggccgcaaat ccatcggcat cttcaaatcc tggatcggca
    48421 ttttcaaaag atgtacagtt cttgactaag ttatgcgcgg gccatgcttc gaaaggatca
    48481 tttgagctac cgctgatttg gattcctgta tttccattcc gataagcatg aatctcttcg
    48541 atcaaattat gatttccaga aatctgtaat cctttttgca tagctgcact atttgtaatg
    48601 tcaaaccctc gtaaacccca gtagtgactg aacagtgtca tcccatttcc agttccttta
    48661 aaatcaagta caggacgctt gttttctgga tttttctctg caatcaactg aatcgggttt
    48721 tctcttgtcc cgttaacgcc ttttggaatc gtcaatcctc tttcgaagga ataagttcca
    48781 ggcgccatga cgattgtctg cccagcatat gcgtatctca atgcttgtgt cagagataat
    48841 ggattttgtc tgcttccgtt tcctgcatct gacccttctg gtgttacata aactgtcgaa
    48901 tatttaggtt tacggtagtc cactgtatga agaatctctt ttgtttcata actagtcatc
    48961 tctacgtatt cttctggcgg atagttttta tctggcgtaa aactgaaagt aaattcgttc
    49021 gttccttcca tcagttttgt ctgataatca aattcccgat tcgcagtgat agccaagttt
    49081 tttgcgttct taagcacctc tcctgttgct ttatttttaa ttgtcaataa tccgtcagca
    49141 tttgctctaa aggacatagt atgatcttgg tttcctgaat gattactaga ggtcacagtg
    49201 taaatcggat caatcaagcg agtaggtctt tcttgcccag gaggatctgt cgcaggatct
    49261 gagtaagcaa cttcgattcc ttctaccttg atcgtcatat tccttgctgc agcaaatcct
    49321 gcataaataa ccgattcatc tgaagcgtat aatttttccc agtcatacat gatgtcagaa
    49381 gccatttctt ctcctgtttc aggatcgtaa taatatgcct ctaaaccagt attcgttttt
    49441 ttcatctcta cgtccagttt agtaatcgag ttcgtggccg gaaacagatt cccatgaccg
    49501 tttccgaata tattataaga acctgtttct ttttccaaga accctgctga agtctctaaa
    49561 ggtgttgttt ccactgagac actcccagga gctggcgcag cttgcggatc ttcagatgat
    49621 attcctgtga ttgcccgtgt ccccaatcca agtcgcatcg tatatttgct gccgctcgtg
    49681 ttgaccactt cttgtctatc cgagtcccag acatattcga tccgactgcc tagtatggca
    49741 aatgaatttg aataaaatgc ctgcccaaac aaatgatttt ttggtactgc gtctcttacc
    49801 attaaagcaa atccttcttg ggcattcgta taggtccaag attctatttc gatcgtcgcc
    49861 cgcatcctaa agttgatgtc tttggggatt gcttgataat aataggtcat tccatctgat
    49921 ccgcttgact ggaattttcc gccgttattc gtggaagaga gccgcacgcc gtcttctaat
    49981 ggttcaatgg catacctact agcacttgtg ctttctccga aaacaacccc tttccactct
    50041 tcatttgctt cttcagattg cttttttgtc ttttcatttt ctgattcttt catcatagga
    50101 tcatttcctt cttccgtatc catatcttca ctagttcttg gtatttcctc cattgttgac
    50161 atcccttcaa tgttcacgtg tgcattttct tcgttcacag actgttgttc gttagcagca
    50221 acttcaacga cagaaattaa aaccggggta ctgagcataa ggagattcat cattagcgca
    50281 ctgtatttta tacgtttttt taacaagtgt attcctcccg taacaaatta gaaagcgttt
    50341 acttaaagta ttacctaatt tatttttact gtcaataaaa aacaaaatta taataagtgt
    50401 attttataaa aagaaaaaat ataacttaag ttgttgataa taaagagatt tattaacgga
    50461 aaaagaataa gtttttttaa aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt
    50521 ttatgctctt cttttattat tttgtgcttt ttattttatc aaatcacttt gtttttttat
    50581 aaaatttctg cgcctagtgt ggactcaaaa tgtctcaatg cccattcatg tccaacggga
    50641 tcgaaacttg ccacggaatc tttgaaaata tggagtttgt atcctagatt atacgcatcg
    50701 actgctgtat gcaacacaca gatatctgta catacgccgg tcaaataaat atctgtgatc
    50761 tggcgttctc gcaaacggat gtctagatct gttccactaa aagcggaata atgccgtttg
    50821 tctatccaat aaacattttc ctcttgctca tagtcttggt ataaactttt taaagagccg
    50881 tacaattccc gtccacccgt tcctatcaca ttatgcggag gaaataagcg gttctccgga
    50941 tgaaaggaat cttttggatc atgcgcatca atggcaaaaa cgacaaaatc cttttgatca
    51001 ataaacttct ttgtatgagc agtcagcttt ttttcaatta cttgtcctgc tgtcccagta
    51061 gtcagctttc catcctctgc aacaaaatca tatgtgtagt caatcgagat caatgcgttc
    51121 atcttagtcc tccttatacg agatgtttga caaattcgat caccatttct gctgatcgtt
    51181 tgcctgcgtc ttcgataaat tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc
    51241 tcattgcacg tacgatcaaa aatgggatat tgaactgacg agctgtttgt ccaacagcgg
    51301 ctccttccat ttcgcaagct aatgcttcag ggaaattcgt taagatttct ttgattttat
    51361 ctggcgagtc gacaaatgta tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg
    51421 ttgcttttgc tgctttaacc atttcactgc gcaaatatgt gcttgcttca taatatagag
    51481 gcattcctgg taattgtcct gggttgtagc caaatcctgt tgcatctgca tcaaaataag
    51541 ctactttgtc tgatataacg atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg
    51601 aacctgtatt gatcaccatg ttcacgccgt attgttggat caacagactt gtggtaatag
    51661 atgctaagac ttttcctata ccggaacgta cgacgatcac ttcgtggtta cctaatgaac
    51721 ctgaaataaa taaagcgcct gcgcgttccc atgataatgg ttcgcttagg ttctctctca
    51781 aaatttttac ttcttcttcc attgcgccga taataccgat tttcatcttt ttaattcctt
    51841 ctttctatat aaatgcgatt gctaaaaaaa ccacacataa cattaggata acaataataa
    51901 gagatttcat caaaaaagag ttccatttgg tcgtcttttc ccgttcgctg atccttgttt
    51961 tcgtgattgg tttattcttt ttgctttctt tacgataaaa gctggcaatc tttttttctt
    52021 cttgctgata agctgtttct gcttttcgct gtttttttaa tgactcgctt gcttgtgctt
    52081 gttgtcgctt gcgaagttcg cttcgcgtaa taagcggtcc tttggccata agccgatccc
    52141 tctactttcc tttggtcaat agttcccagt attgaatcgc ataaatcgtc tttgcatcac
    52201 aaatcaactg atctttcatc gcttgttgcg cctcttcaag agttagatga tacaactcca
    52261 gtacttcgtc ttcatcttgt gctaaaggat tctcgacctt ctctacccct tgtgcatgat
    52321 aaatatgaag gacttcattt gcaaaacctg gtgaaagata catcgaagta agatggctca
    52381 gcgacttcgc acgatagcca gtttcttctt ctaattctct agctgctgtc atttcgggat
    52441 tttgtccttc tcctggatcg atttttccag ctggaatctc caaaatcact ttttctaaag
    52501 gttttcgaaa ttgtttgaca agaagcagac gatcttgttc gtcaaaagca atgatcccga
    52561 caccgccagg atgaaatacc agttctctct tcgaagttcc tccatctggc aaacgaactt
    52621 cgtctacagc aacatcaata atttttccgc tatagatttc tttgcgactg attgtttttt
    52681 cttcaaattg ctcattgttc agcatatctt ctcctgcttt ctttgtaatc ataactgctt
    52741 ttttttcatt tttcaatttt tctcaaaaac tccttattag tttaccatag ataaacctta
    52801 gattttctct aaatatagaa aatccttctt tagtccgata ctctttctag tagtccgtat
    52861 gctaaaataa gtatgtaatc gaataacgaa aagaattcaa tactcaacga taaatgacat
    52921 ggagggttta ttatgataaa acgaaattgg ccgtggcttc tagcattggt cttgctattt
    52981 ctctttgtcc gaaacaatag tttttctttt gttctaggac ttttattgat aggtgtagtt
    53041 ttagttttgt ggggactctt tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct
    53101 gcactgactg acgaacttga aactcactat gaaaaaagcg gtatgacagc gaatgaaatt
    53161 tcattttttc gacaaacaat gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat
    53221 atgcagcaga cagccaaact taaagcaatc gatctccgcc atgatccagt caaagcagcg
    53281 aaagctttat tcaaagaatt ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt
    53341 ctttatacgc atctgccgaa tctagttgat ttgacaaata agtataatga gatcaatgac
    53401 catgagatca aaaacaaaca gacctatgaa aaactagaag aaagcgcgca gatcatcgac
    53461 cagctgtcag ctttaatcgc acaagattat caaaaattcg ttgctgagga tctagatgat
    53521 atcgacgtag agatatctgt tgcgaaacag agcttaaagc gtgataataa atatgaacca
    53581 gaacaaaagg attaactctt ttgttcataa aagaagactc ggaacattca gatgatgtct
    53641 ggctgtttcg agtacttttt ctaaagtaga gacattcatg taagaatcga gaacagattc
    53701 aacaaggagg aattttttat ggatcataaa ccgactgaaa caaaagatgt tacaccagtt
    53761 aacgatacac ttgaagattt attgaacaac ccgttttcaa ctccaacaga tacgctgacc
    53821 acttcacaac aagcggagat tgatcagctc caagagcaac agacagctgc tcgtttaatt
    53881 gacaaactac cggcggaacg ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct
    53941 aatgatgcac agtctgtcat cagctacgga tcggctgccc aagcaaaatt aagtgaattc
    54001 tcacaatcga tgttgaatca tgtgcaagca caagatattg gtcctgtagg agattcctta
    54061 actgaactga tgtatcgtct ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg
    54121 aacatcttcc agcgtgtctt cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa
    54181 tatcaaaaga ttggtgcaca aattgacaag atttcagtga aattagataa agaaaaagac
    54241 ggtctgctaa aagataatct gatgttagaa cagctttacc agaaaaacaa agactacttt
    54301 gatgcactga atatttatat cgctgctgga gaattgaaaa tggaagaact tcaaacaacg
    54361 atcattccag aagcgatgaa aagagcagaa gaaactggcg atcagatgga tgttcagatt
    54421 gccaatgatt acacgcaatt cctagatcgt ctggataaac gtacgcatga tcttcgctta
    54481 gctagacaga tcacgatcca gcaagcacca caaatccgtt tgatccaaaa cacaaatcag
    54541 gccttggctg aaaagatcca agcatctatc gctacagcca ttccgctgtg gaaaaaccaa
    54601 gtcgtgatcg cattgacctt gcttcgtcaa aaagatgcag taactgctca acgccaagtc
    54661 tctgaaacaa caaacgactt attgaagaaa aattcagaaa tgctgaaaat ctcagccatc
    54721 gaaacagcga aagaaaatga acgtggaatc gtggatatcg agacccttca aaccactcaa
    54781 aatgatttga tcgaaacaat ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa
    54841 cgtcgtcatg cagaagtaga gcttggacac atggaagaag atctgaaaca aaaactgttg
    54901 gatttaaccc aatagatcac ctttcgcttg ttactaaaac aaacgcccaa caacagttta
    54961 ttacaactgt tgatgggcgt ttttcttatc tttcttctaa cttctccatt ggagtctatt
    55021 tctcattcat cttgctgtgt ccatagccat agccaaaata aatgatcagt ccaataacca
    55081 ttacgatacc gaacacgatc catgtcacag tttgcagacg aagcatcaat acaatacaag
    55141 tcaagatgga gacgattggc aatagcggaa cgaatggcac tttgaattct ccagcttttg
    55201 gtttgccaaa catttttctc aacttcagta agcctagact aacaagcatc aaataggcga
    55261 gcgccatgat attcgttagt tccgctaata cattcaatgg gaaaaatccg gcaaagaata
    55321 cagctgccac accagcggca taagtcgctt ttttcggtgt acgatgcttt tgatcgattt
    55381 ctgtcataaa cttaggaagt aagccatcct tgctgacagc aaacagtaaa cgagcaagtg
    55441 aatacatcat cgagatcgtt accgtcagta gcgttaaaat tgcccctaca gcgatcaccg
    55501 cgcccactgt tccatgtcct acatacctca ttgcaaaagc cactgggtca ttgactccta
    55561 gttcagtata cggaacgatt cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag
    55621 caatcagtat cgaaccaatg attcctcttg gcacatcttt ttgcgggttt ttgacttcct
    55681 cggctgccat tgcaacggca tcaaatccca agaaagcaaa aaacacaaga gctgcaccat
    55741 ctagcacacc ggaaaaacca aaaggcatga atggctgcca gttatcaggt ttaacgaaaa
    55801 atattcctac aatgataaac aacgcgatga taccaaattt gacatatacc atggcattat
    55861 tcaaacgtaa tgctttcttg gcttcaagcg acacgatgta agtgacgaat atcactacga
    55921 gcaccgcaat caagtcaatg tatgtaccat tttccggatt gtacccgcca gttaatgctt
    55981 gtggaagttc ggcccctaaa cttttcaaaa aaccttgcat atagccagac catccggaag
    56041 caacagagga aaccgctagt aaaaattccc cgatcaacaa ccaccctgtc agccaggcag
    56101 caaattcacc aaatacaaca tacagatagg catatggccc accaatcact ggcactcgcg
    56161 aagcaaattc tgcgaaactt agtccagaca aaatgacaac gattgcagct aagacaaatg
    56221 aaagtgacag tgctggacca gcattttgat tggccgcaac ccctgtcacc acaaatattc
    56281 ccgtaccgat gatcgcacca attccaagca tgatcaaatc agacgttttt agttcttttt
    56341 tcatctcact cgtatgattg agattgatct ctttcttacg aaacaaatcc attgaagttc
    56401 ctccccattt taggttggaa aacagcgatt aaactgtttt catataccct aaaaggatac
    56461 cacctaaaat gactaaaagg caaccaacaa taacataaat gagctctttt ttcgtttttc
    56521 tttcgccaag gatgaaaatc ccacctaaag tagagataat gatccccatt tgtgaaagtg
    56581 agaagccaac agctaaacca agtgatttga ctgttagaag catgcagatg tttcctaagc
    56641 cccataataa tccacagatc atgtttttcc acacaaactg atcgacttta actttacgga
    56701 aagcaaagaa gcttgcgcct aagatcattc cgatactttg aggtaagata attgacatcg
    56761 catccaaatt cgcccagttg acgatgatgg tatatacacc ataaccaatc gtagagaaga
    56821 tcaatgctct aaaaccctta ccaaaatcaa gcattttatt atctgtatct atttttcctt
    56881 cgtcatcttg tcgggcagtc aaatacgcac cgcttacaag aagagctaaa gcaataatac
    56941 cgtatacata atctttcgtt tgtgtccatt catggaagaa aacggctccc gcaatcgtat
    57001 tcaaaattaa ttgaaaacca gtagacaatg gtagccctac agaaacaccc atgtacttca
    57061 ttccatggaa ctgtccattt tgtccaacac tccaagctaa gccggataaa aatccgatca
    57121 agaaaatcca aggggtgatt acaggactaa caacgaaaaa gacaactaaa gaaaacagaa
    57181 atgcaccgat cgtcattcct agtgtttgtt gattggcatc gccaccaatt ttaccactta
    57241 ctaaaccaat actaccccat gccaacatag ggactaatgc tattaaaatt tccataacca
    57301 atctcctttt atttcacatg ataagaaact tatcagcgaa aacgttaaca acacaagcac
    57361 tttttttcgt ttctaacgat tttctaacgt ttttcattat aaaaatttaa gattgggaaa
    57421 aataaagtca ttgttgtccc ttttccttca gttgattcag ccgtcaaatg gatacctaat
    57481 ttttcggcca gattttttgc taaataaagt cctaatcctg ttgaatgtgt tttgctaagt
    57541 cgaccatttc tcccagtaaa tcccttatca aagatccgtt tcaagtcttc cgctgggata
    57601 ccgatccctg aatctttaac tgaaaggctg actccttgcg cttccttttc tatgctcaca
    57661 atgatgtttc cgtgatccgg cgtatatttg atggcattac taagcaattg gttgaagata
    57721 aaagcgatcc atttagcatc ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga
    57781 atattttttt ggataaaata atttgcctga ctgcgtatca caggctgaat gattcctttt
    57841 agtccatatt cttggatgag gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa
    57901 acttgttcta cgtattcatc gatttttgat aattcatttt ctagtaatgt atacttttga
    57961 tcgtcaatat cgaactcgat cgaacgaact agcaatttag aagcggaaag tggcactttg
    58021 atttcatgga cccatgagtc aatataatct ttttggtctt gttgattact gattgctgat
    58081 tgcattactt cttggtgttc acggatcagc tgattgatat attcttctac cagtttttct
    58141 tcttctgtcc tcccaccact caaataattt tgcaaagggg aagttttttg tatgtcggct
    58201 aattttcccc accatcttct cttgctcaag taaccaccca gcagataaaa aatcagaaag
    58261 acgccctcaa tcaaagctaa ataagcaatt gtgctcaaat caactttgat gtttggcgcc
    58321 agccacatca caaagatcgt caatgcaata aaaaacagcc atcccaagat caatgtccac
    58381 tgatctttca aatatttaaa aaaattcatc ttaaccgctc atcctttcag ggaacgatat
    58441 atccctgtcc tacttttgtt tcgatatatc cttggatacc tgcctgttcg atttttcggc
    58501 gtaaccgatt gatgttgact gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca
    58561 gtgctcgcag caatttttcc cgacttaaga tctttccatt gtgcttcatc aaaataaata
    58621 atagacgata ttcatttttg ctcaaatcaa tgatctctcc gttgatttcc atacttccgt
    58681 tgtcaacatt caacgtaatg ccattgtggc tcatcatttc actagataat tctgtgtagt
    58741 tgtaagaacg gcgaagtaat gcattgattt ttgcaatcag tacatcgatt tggaaatgtt
    58801 ttgtcacaaa atcatctgcc cccatgttca tagccatgat catatccata ttcgtattcc
    58861 tgctggagat aaaaatgatc ggtactttcg atacttcacg tattttttga caccaataat
    58921 agccatccaa aacgggaaga ttaatatcta gtaatacaag ctgtggttct tctctttcaa
    58981 agtctgagaa cacttgattg aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa
    59041 gttcctccat gatcaattgt cgaatgatct cttcgtcttc gactaccatg atttttgcca
    59101 taaaaaatcc ctccatcttt cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca
    59161 agttggctta cctggaaaat gtcattttag caaacgttaa aaaaaactgg aaatatgcga
    59221 tttaatcgcg tttgtctctt ccaagcagct taaatttatt atgttattct taataattgg
    59281 tctattatct gttatttttc tttcgttgtt tttgtctttt aaattttcga taacaccaat
    59341 aaccgaaaag cgttagcact agcaatatac ctagaaggag tataagtaat cgataaagat
    59401 ggtatttttt tgtctgttct atctttgaag agacttcttc tgtaaaggga actcgtcttc
    59461 ctgtcactag taatcggtga gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta
    59521 gatcctgctt ttctctaatt gctaatgtat ttgtttttgt tggaaggaca acaattttct
    59581 caacgacttc ataagcgagc atccgaccat acacttcaag atagaatcgg tcatcttgct
    59641 ccatttcttc aagatccgta aagagttttt tctcagcaat accgctatgg gcggtaatca
    59701 cggaatgtgt actctctcct ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc
    59761 tatctaacaa aagatcattc gtttcatcaa ataaaggcaa gctaacattg attttcggaa
    59821 tatagatggc tccgatcata tgttgttcgt aatattcttt tcctggattt tggacatttc
    59881 taaccgcttg ctcaaaagga tctttgactt gtcccattcc tgggattact gatgtatgag
    59941 caagcgtctg atttttcttt tcatgcactg ctaattgttg ctcatgtttt tcttctttat
    60001 cgcgttcaag ctgcttttga taattctcga tccttctttg atccaaataa ttgctgagag
    60061 catctgctaa aaatggatat gtaaaaactg cagctccggc aagaaacatc actgccataa
    60121 ataattgtaa taaaagactg atttgggttt gaggtctatt acctattttc tttttcattt
    60181 tcttcacctt cttatttaaa aaaactgcag caacctttat aaaaccgatt gctgcagctt
    60241 atttttgttg cttatacttc agacttcatt ttagatttac ggtaccagct gtacgctcca
    60301 atcatcaaac ttattccgat tagtaagaat gccaagagcc cgttcccacc agtagcagga
    60361 agtgacccgc gtttcatatt cggtactctt tctggccctg gattttctac tcctacgatc
    60421 tgaatttctt cattatagct tccataagag atgataaatt cagtaaaggc tgcctcacct
    60481 gttggaagta cataaccttc tggcgccatg gtctctttca tttgataggt tccataatct
    60541 aacccttgaa cctcaaagta tccatttcca tctgcgacaa attctgttgc ttgtccgctt
    60601 gttgcccact caattctatc tgctcctgat tcataaacag ctaaaggcga ctccccttta
    60661 taaaatattg cgtattcaga acggtcttca ttccacaatt cgaatcttgc atctttaagc
    60721 aattcactgc ttgatgcatc atatttttca aactttcttc cacctgttgt gactttctct
    60781 ggcggctcta ctccttcttc ataatcaaat tcatcatttc gtccgatttc aataaccgct
    60841 gtgttgttat ggaaatcatc cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc
    60901 gttaaatctc ctccacgtgc atctgctaat ctacctaaag tatctacttt ggctactcca
    60961 ttttcttctg tcaaaatcgt cacgacaaac ccgttgctta ctacttctac tatataatct
    61021 tgtccttttg ttaaaatcgt ctcgccggct cgcacttcta tttcctgatc ataataagtt
    61081 agaccaactg taggcgtatc tgtgattttg taatttttga taacagcgct tccgttttca
    61141 aggacagaat cgatgaaata aggaataggt gctgtgatcg tgtaagaagc cgtttttcct
    61201 ctttctagat caatataaga aatcgtttct tgttctccag cttcattaat aatcgttact
    61261 tgtctaaggt cgctttcctc tacattcatc tgtttatctg cttctctgat ttcgtttttc
    61321 ggatacaaat gcacatctgt attgtatgtg tctttccatg tagaaccatc ccacacgcca
    61381 tccaccatat caggcatcat cactggcatc ataacaacca ttggagacgc gattctggaa
    61441 atatgagctg gcgaataagt ttctaagaaa agatagactt tatctctaga agaaggtgct
    61501 tcttccgtca cttggacggt agagaatacc gcttctccag cttcattcgt tgtttgtttg
    61561 tcaatacttg tcagtcgata ttttaaaaac caactttctg tgtgccgttt ttgaatccat
    61621 tcaatcgctt cggcagaatt aagaccgtct tctcgttccg gtgttaaagg attatctcga
    61681 atcaaatcgt aatatacttc cgttacgtca aacaaatcaa aatcgactcc tggaatattt
    61741 tcttcaccaa aatccgtaga aaccaatccg ctattcggtc gttcttccgg aacagatgag
    61801 aaaccttttt tgtgtaatgt aatcgtcact tcacttggtt tttgtgcgac ttcttcggct
    61861 ttgactagtt ttccgttccc gaaaatccct attcccgcag taacgagtaa cagtacactc
    61921 attcccgtga caatcatttt tttattcatt tttttctccc cgttcttttt ttattaataa
    61981 aacagcacct aacaataaaa gaattcctag taaagaaatc aacgttcttg cttcattcgt
    62041 ttgcgggaat acggaccatt ttcctgattg acctggattg cttggaccac tcggctcttt
    62101 cggtttgtcc gttgtttctt ttacggtctc gttataaaca cgtggtgttg cggattcata
    62161 ggcagagact ggaactttcc cggattctaa ttcagccatc tcctgtccgt taactactac
    62221 atattgtggt tggccttttg catccaccca aaattcaggg atgatgacct cgattctctt
    62281 acttgcttca tccatctcgt aaccttccac accttttaat tcttcaaaat aataggtgcc
    62341 agaaggcaag aaacgttgtc ctgtcgtaac aaggccctct ttatccgaaa tgaatttgct
    62401 tacatttttg tcattcaaag gatcattgtc tgctggtttg atccattgat ttttcaagtc
    62461 attagcagga gccatatcta aataatattt ccccccttct atcatctgat atagagcaaa
    62521 aacagcacct tctaatggtt ttcctttttc agttgttcct ctttgacgtc catacttgaa
    62581 gaagtatgga tctcttagat aaccgacatt tttaggatag atatgaatct cttgcagttc
    62641 ctcggttgat tctgtcgggt gataaattgg caaaatagca gcaattggga tagctgtctg
    62701 atcgatatca acatccaatc caacttctgg atcaatcgat tcttcgaaaa tcaaataaac
    62761 tgcatcccga tcttggtttt tcttaggtgc tttgatacgt gcaatcccgt ctcctaatcc
    62821 gccaaccgta tctttcccag ttgttaccga agtccctatt tggacaagcc tttcttctgt
    62881 gattagctga cggatattgt ttctgttcgt ttcagcgatt tgctggacga attctttagg
    62941 agtcatgttt tcagcagctt ttttttcatg ataataatcg gtcatgtcgt aaatggcaaa
    63001 tgtcgctcca tttagtggca ccgtctgtga aatgatatct gctgtttccc ctgcttcctc
    63061 agagatagcc aagccatcat tttcatggta ttcaaattga tcgttataat cgatatctcg
    63121 aatcatccgt ttatgcaaca cgaattccac gacatcttcc cctgattctt ctgccgacat
    63181 cgcatgtgga agaaggactg ctgcaagcgg tagtaaaaca agtaattgaa gcatcaaaca
    63241 caacagcttt ttcattacct tgctccctcc tttttagatt gtctgacaaa caaaccatag
    63301 gctgctcctg caagcgccat gaagaatgca gcaagcagga tatagaataa tcgtccggat
    63361 cctcctgtag caggcaacgg attttcagga tcgttcccga cactaagctg taatctgatt
    63421 cgatttgtct ccaaaagttc atattctaac tcttgctgta gatcaggcgt ctctgcttct
    63481 tcgtttggat aatacgaaat ctcaactttt agtgtacccg cttcttttcc tggaactacc
    63541 cgatcatttt ctatgtcagg ttcttctcga taactaattt ctaaaataaa gtaacctgga
    63601 agttttctat agctgtcagg tgacttcact tccttgatcc gatagacccc ttccaacaag
    63661 tctgaagcat tgaataggga agaatcgttt ggatctggtg ccaagcctcc ctcgatgatt
    63721 tcatactctc ctgcactatt ttgtttctca attgaaaatt ctgcgccttc aagacgtcgg
    63781 ctattcatac tgtcaatttt caaaagttcc aaatcgattg gcgctagttc atcttctaag
    63841 gttacttgta gtacaccatc gatgatttca tagccttccg gcaagggagc agtttcgtct
    63901 tcgatgaccg tatcttcaat gataaactta ccttcttcat tcagtgcaaa ctggaacact
    63961 tgatcagata gccgataccc atttggtgcg acagattccg agacctcata ttcaccaata
    64021 gacagatgca gaggttggtc gtaatttaaa tcgacatact tttctactcc tacggcacga
    64081 gaatagaagt atccgatccc aggcaaacgt ttcgattctt ctggttctaa cggattacca
    64141 gtgtatgctc gatacagcgg gaaggaagaa tcccccactt tcctgatatc gaaacctgca
    64201 ccatctaatg tatggccttc atggtctaat tttctaactc ggatctcccg ataatcaaac
    64261 gtattgtaag cagtgaatgc tacccataaa cgatcctctt catctgttgt atacgtgaga
    64321 tattgccaat tttcttcacc tgtttgcttg agttggatag tccctatagc attatcgtaa
    64381 agtcgcaaga tccatgtgtc attttcatca ggttctttga aaccttctgg gttctggctc
    64441 tcaatgattt tgtattctgc atagttggta gtttcttcac tatccttata aacaaatgaa
    64501 tctggctctc cgattggatg aataactttc tcaccatcca cgattacata tttcccgtct
    64561 tcatctactg aatagaacgt gagtctccct ttctcttcct ctacttcgtt cacaccagaa
    64621 agccctttcg ctactggata tccgatttcc agtccgttac catctgtttc ataaagttca
    64681 aactctgcgc cattgattgg catattactg ccttcaatta gtttattgat attcatgtgg
    64741 attggcttga attcatttcg catttccagt ttgacataca actcatcata ctctgtgcct
    64801 tctatgttag gctcttgtac aacagaaagc ttgtgatatt ctccttcata ataggaattc
    64861 ggatctttac cttccggtcc gccgactctc gaaccagatg tatattcatc tacaagctcg
    64921 tagtgaccta acatctcatc ttcgttggct gcatattttt tgatttggaa ttgatacgtc
    64981 tcatctacta actgataacg tggtggtcca gcgatctctc tgatccaaaa tatgtcgctg
    65041 actgctccag tttcatctcc taaaatcggc atcttcgttt caaagatcgc tattccgtcc
    65101 gtggtagttt tacttgtttc gattggttcg gcttccccac tctctggatt ttggtcatat
    65161 aatgcaaatt cgactccagc caacggctcg ccattttgac ccttcttgct gacttggaaa
    65221 ttacggcctt tatgttcgta acgaatggaa gaagatggga aatacatccg tccagtagtg
    65281 atatcattct cataatcctc caggttctga atataggcca tattgttcac tgtgcaaaaa
    65341 tttccgtctt gagctcccgc atcaagtttt acttggtact tgatcctgac catttcccct
    65401 tcacctaaag aaatcggaga aaatttataa gcttggtttt cccgtccgac atacgttgtc
    65461 ggagtcgcca ctacttctgg tggggcttgt ccatgacgtt cttcatccca tatttctggc
    65521 tcttggtact tcatgccgtt ttctcctgga tctagatggt aaaactgaat atctgccgat
    65581 ctgccagctc caaatggtga gacattaaaa ccagtttcat cataaagtgt ggcttgctgg
    65641 atcgttctct tgaaggtaga agagatatcc tccaaggctg taacaatgtc attcttgttg
    65701 ttcgcattgt aatatcgcgg tgttacgcca tcttcttcta aatcagcaat atttttcaat
    65761 acatttcgtc caatagcatc tagtcgttgt ctcccgctaa cgtttccatc gattcctaaa
    65821 ccaatggaga agatacttgg cgggttctct tgatacttat ttttcagcca atgatgataa
    65881 gcaactgtgt tgatcgcaga tgacttttct tgcgaagtag gactgccatt cgtattgtta
    65941 ttagaagaac catcaccaac ccagtgagtc gctttattat ctccttcttt tacctctcca
    66001 taacgatagt gtacatttac ttcatcttct ggacggttaa agtcagatgc ataggtaact
    66061 ggatgagtaa accctgcatt agaagtattc ccagaaaact cctcaaaatt tctgaacaaa
    66121 acgtcattat cttcagcaat cttatcttcc atcacagtcc agttgctcca agctccatta
    66181 gtgctcgtac gataacttga ctgaatcggt gcataactga aggtcggtgt cccatctccc
    66241 acaacgatta ggattttttc tggatttcta ttttctccgc cattgtcttt atacaatgtt
    66301 tcatagccat ttttaagccc tagtgtaagc ggagttcctc cgattggtgt acgatttatt
    66361 gctgtattct cacttcccgt caaacgattg atatcatttg tcaatggaaa gatgtccgaa
    66421 ctgatttgct catggttatt gatagctgta ctattatggt aaaaattcac cataccgatt
    66481 cgaatatcca gactcgggtc agagagcaga tctttactca tttcgtttac tgcctcaatc
    66541 aacgcagcat ttttagtttg actattggtc ccagcggtta attcactcat actcgctgac
    66601 ttatctagaa caaaaacgat atcgattgga ctgatttcct ttttctcgcc cccgatgaca
    66661 tctagataga cattgtaaag ggttgttgca tcttcaggat gtagatcaga tgctgtgctg
    66721 cttggcagaa caggttctac ccattttttg atgtacgccc cgttataatc caagtaactg
    66781 ttttcaaaat tccgttcatt tccccaaagc tgaacggttt gagctggaac gtttctatct
    66841 tcttcgcctg cagttgatcc attattagta gcatagtcgt aatttcgcca gttcgctccc
    66901 ccgtttgtat aatcactatc atcgatcaag ttctctccgt catatggcga gcctttgtct
    66961 ccttgatagc gttcattgct ttgtgtccaa tttcctaaca cttttgtata gtcattcgtt
    67021 gtaaacttcg ggtagcgatg ctgttcgttt tcaacttctc cgctaggata gatatattca
    67081 taggggtctt gcgtagctgt tggcccaaga cgctccaaaa taccgttgaa taactggcgt
    67141 tgaatgcgct ggagtgcttg accgctgggt acgattggtt cgtccagctt ttccatctca
    67201 ttttcttcgc tacgttgaat ttcagcggaa tcttctgtat cagtttcatt ttcttgtaat
    67261 tcttggttat ctattctttc atcaaccacc acttgaagag gtagttaccc ttcgttcaca
    67321 tcggggatct tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt tgaataaact
    67381 tttccgacat accattcttg accatcgatc gtctcgactt ttaggtcttc tccatcttca
    67441 tcataggagg atagacctgt tccacgaacg ttttttacgg taccaatccc ggcggctgcc
    67501 gcatcgattg ctaaacgtat tctaccttca ttggcacttt cctttttttc aaaagccaga
    67561 tgccattcaa gggtttcttc tttttcaata tacttccctt caatttttgc atagtcctca
    67621 tctaagaata cctcatcctc ctttgtttct gttgactcag cagctacatc cagggtcgtc
    67681 atatgataga ctgactggaa atatggtcca aatagcaaac tgacagttac taactgcaaa
    67741 agcttcaatt ttaacccttt cacatcctta tactcctttc aaaaatattt gtcttctttc
    67801 acaacaatca acaaaacaat gcccatctaa ttataattta atttaatttt aattttaaat
    67861 caaatgatat cctttttagt taaaaaacac caattataca acatttatta ttaaaattaa
    67921 attataaaat tttataacgt tctcatttaa caaaacttaa gttattaaga caaatgaata
    67981 aacaactacg ctttcctcga aacaaaaaaa taaagcacaa atgcgcattt acgcactcat
    68041 gctttattcg tacaattaat tcagataacg gcagcgcata ggatgccaat aacacttcct
    68101 aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa gtaaaaaaat cctcgaataa
    68161 gcgatcaaac aagcaaataa aaaggctaat attcctgcaa ctggacttaa ttcgaacaac
    68221 gcagctactg ataaaaaggc tagcgctgta tgtcctgaag gaaaagaaga agtcttcggt
    68281 ttacttgtgt aaaggacaag ccgacgattt ttttcataag gtcttttccg tcttgtcgtt
    68341 tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac tgccgataag aatggctaga
    68401 ccatattctc tagtatcagg tatcaagact aaaacagcag caaggagcaa ccaaataaaa
    68461 ccaccattag agatattact gataaatttc aaaaaattga tttttcgact atccgttttc
    68521 cgttgcttgg cgaacttgtt gataatacta tcatcgattt tagcaatcaa acgcaacatg
    68581 ttattcctcc tttttaagaa ccttttcttg cgtacgatgg ataaaaagca ggacttgaaa
    68641 ataaaacaag aaactgaaac ctaataaagc aaaatagatt ggtcctgcaa accagaatgc
    68701 gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg cttttgaaat cattaaaaaa
    68761 caagaaaaat gcagtaatcc aagaagcttc tttttgttcc agttgaagca agattttgta
    68821 actaaagaga agcgcgctta atagaagcaa aaacaacgta gtatacatca atgtatagcc
    68881 aattccccca ccccaatagc ggaaaatctg ataatcgatc aatagaagaa gaaaataaac
    68941 acttgctaaa ctatcttgct ttccataaag agagaagttc tcacgaaact ttttccagaa
    69001 tagtgaaccg aactttttct cttcattttt tttgtaatga taaatcatat acagaactgt
    69061 tcgacttgca gttcctaatg tgaaaacagg caagacgcac aggaaccata gtagctgtaa
    69121 atggataaga cgtggaatcc attcaatgat ttctaagaaa taatcattgg cttttttacc
    69181 aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat ccatcccttc aatgaaataa
    69241 cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga caagtgttgc tcctgccatc
    69301 agataattcc attgtgtcgt tgcttggttc tgaaaaactt gcaagccaat ctgtaacgtg
    69361 tatttttctt gatcttggat atataacaga ggacctaaga aatcattcca agctgcgttg
    69421 aaggaattga tcgcaatggt aatcaaggca ggtttcgtca atggaagcat caaatgactc
    69481 caaatataca aatgattcgc tccgtcgatt tcagcagctt cgatcaattc attattgatc
    69541 gataagtaaa attgacgcat cagaaatata ttgaacgcat tgccgaagaa cgatggaacg
    69601 atcaggggca aataagtacc tacccagccg atcttggaga ataatacata ttgagggatc
    69661 atcgttacaa accccgggat catcatcgta gaaagaacca aagcaaacat cagctttttc
    69721 ccaggaaaat caagcttcgc aaatccataa gcaataaaag aatttgaaag gacattaccg
    69781 atcactacta gcacagtgat gaataatgta tttcttgcat agcgccaaaa aggaaaggcg
    69841 gcaatcgtat ccagatagtt ttcccatacg attttttctg ggaaaaaggt cggaggataa
    69901 cgaacgattt cttccattgg tttcaatgaa gtaaacacca tccaaagcaa tggaatgatc
    69961 aaaatgattc ctattactgt catgaggaca taacttagta ttttttttgt ttgccgattc
    70021 acactggtct ttttgaaata atccatcatt tgccgtcacc tccagaataa tagacccact
    70081 taggtgcaag cttcaaattg acaaacgtca gaaccaacac gatcagaaac aggatcattg
    70141 acatagccat cgcataaccc atatcaaaag ctacgaacgc cttgttccac atatgcagat
    70201 tataaaaaag caaagaattt gcaggtccgc ctgttccatt tttcgtcatg acataagctt
    70261 cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac atcgtaaaaa ataataggag
    70321 tgattaatgg caaagtaata cttttgaact ttccccaaac gcctgctcca tcgatttctg
    70381 ctgattcata taaagatcta ggaacatttt gtaatgtagc taagtaaagt agcattgctc
    70441 ctccagaact ccatattttc atcaaaatca acgcaggttt cgtccattgt ggatcaaaca
    70501 gccaagctgg tccagggata tggaaccagc ccagaaatgt gttgatcaat cctgcagatg
    70561 gagaaagcag ctgcatccat aagatgtaga ctgctactcc tgataagact gccggtaaat
    70621 agaaagctgt acggaataca ctgattcctt tgattttttg attgagtact gtggctaaaa
    70681 acacactgaa aatcgtcgtc agcggtacat tgaagatgac gaaataaacg gtgttgtata
    70741 acgatttcca aaataaatca tcgtgaacga acattctttt gaaatttgct agaccaatga
    70801 aattcatctt ggatgtcaaa ttataatcgg tgaaactacc aataactgaa aaaagcatag
    70861 gccctagcat aaataccaag aacccgataa taaaaggcga gataaataaa taggcccatc
    70921 ttgcttcttt tttctttcgg atagataaac gcttcaaatt ttcttgcctc ctctgtacct
    70981 agacagtcaa aaatggatta ttgattttgc ttgactaagt tttctactgc tttttgtgca
    71041 tctgccaatc cttcttttgg tgattttgtt cctaaaagaa tctggtcaat ctgttcattc
    71101 actaatgtcg tgtaatccgg agcatctaaa ggaactggtg tgatcaccgt atttttaaag
    71161 ttttcgtccg ccatttgata gatcattttt cctttatccg ttaggttgtc attattcact
    71221 aaattctcat tggctgtctt gctcgccatg atatcaaaac ttttttcgcc gaatttttct
    71281 tgtacttctg gagaagaaag atatttgatg aattcataag aggcctcagg atcttttgct
    71341 ccgtatggta cttccaagac gaatccgcca ccccacgacc aatggcctga accttcttct
    71401 ttttctggga gctgcatcac gccaaaatca aaatcatccg gtgcattttc catcaaacta
    71461 gagtaataat tgatattttg ggcacgcatg gctactaatc cggaaataaa tggatcagat
    71521 acaccagacc caaattcggc ttctaattta ttgatcgtat ttttcccata ataatcttgc
    71581 cagtcttgga tccactccaa ggcttccact ttatttttag tatcaatctt gactttttca
    71641 tctttgtcaa accaactagt cccgttatct gcgtttagcg cccatacatc agcccctaaa
    71701 ttccataatg gataaaaacc gatgcgtgta aattcttttc cttctttttt atctagttta
    71761 cgtgctgttt cttctaattc atcccaagtt gtcggtagat cttgttcact gattccagct
    71821 tctttaaata actttttatt ataaaaaatg acttgtgtat ctgtattgaa tgggatcgca
    71881 taaggatcac cttcatattc tactgtatcc caaagctgcg gataaaaatc cttagaagtg
    71941 tctttttcta catatttgga gagattagtt gcttgctgtg cttctgctct ttgggcaacg
    72001 gaatttatgt cctgtacaat aacatcgggt gcatttccag ctgttattgc agataaagat
    72061 ttagtccaga tgtcacccca tggctgatac ttataattta catggatctc gttttgtgat
    72121 tgattgaaat cctcaatgat ttcttcgata gcttcttttc gtgcaccgga tccccaaaag
    72181 gaccagaaat caatattttt tcttccatca cttgtttttt cagaagaatt tgctgcactt
    72241 gaactggcat cgcttgcatt acttgatccg catcctccta ataaaaacaa tgctgaactt
    72301 gccagcaacc ctgtaaacaa taattttttc atattttacc tccaaatttt aatgtaaccg
    72361 atgccggttt taatataacg atatcatttt gttctgaaaa taacaattta taaggtatga
    72421 ttctaaaaaa attattaatg ataccaatac tttatataat tcgcgagcgc tttctttgaa
    72481 ttttttaatc tttcgtagca gaatgagaat gtattaaaaa tagattagga ggaattttga
    72541 tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc agatcgtccg aatccattaa
    72601 tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg cgagatgagt gtagctatgc
    72661 agtacctgtt ccaaggctgg agctgccgag gagaagaaaa atataaagat atgctgatgg
    72721 acattgcaac agaagaaatc ggccacgtgg aaatgttggc gacaatggtt gctcgtttat
    72781 tagagggtgc gcctgttgaa gatcaagaag cagcgatgca aaaagatcca acaatcggtg
    72841 ccattatctc tggaatgaat ccacagcacg cgatcgtagc tggcttagga cctcgtcctg
    72901 ctgacagcgt gggcaatcca tggagcggtg cctatatcat tgcgagtggc aacttgttag
    72961 cagactttag agctaattta aatgccgaat cacaaggaag acttcaagta gcacgtattt
    73021 acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc tgttctcctt gcaagagata
    73081 gctatcatca aaatctatgg gaatgtgcag tgaaagaatt agaagaaaaa gaaggttcaa
    73141 tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa agacattgct tatgacttct
    73201 ataacttctc agagggagac caaagtagtg aaggttcttg ggctagtggc aaagcactgg
    73261 atggcgaagg cgaatatcaa tggttgaaag aacctaagat cgaaggcgct gcaccaaaat
    73321 tagaaccagt aacaccaaaa atgtatggga cacctccatt caagaaataa atatctaccc
    73381 gtagaaacac ccgttttttg ttccacacgc tttgtgagca aaactgacta aagtcattaa
    73441 tcataaccac ccttcaaaag ggtggttttc tctagagcta taagctcaaa tcaccggcca
    73501 gcgcctaaag acgctggctt tcatatttta tgttcaagcc atattgcctt tgccagtttt
    73561 gcccacctct taaaaggttt atgtactact ccttagccct taaaaggatc gtcttactct
    73621 cgtacactca gcttatctaa cgcactatca tgtttttctt gttcttgaat gtactttttg
    73681 atggtcgctt cattttgtcc tacttttgtc acatagtatc cttccaccca aaagtgtcga
    73741 tacccaaatt tatattttag atttgcacaa tctcttgatg atctcctgaa tattggctcg
    73801 atattgatta taaattattt ttcttctata ctttggtgtg aatacaatgt ggtatttaca
    73861 catctacttt gtacgggcta aactatttcc ctttattgct ccacccgcat agcaagtggt
    73921 tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa gacaataaat ctaaaaaccc
    73981 gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa tgaaatgaaa aagatctctt
    74041 acggcaatct attttatttt tttgattttt tataaatgga aaatgcttca agaagtccaa
    74101 gaaataaagc cgtataaaaa caagaaccta tgaaatcttt atttattaga tattcaacag
    74161 aaatcccgat aacactagcc aacaatatat ttatcagaag tgtaaaataa ggatgctttt
    74221 tatagaatag aactagtgtt gttttcatag actctccttt cttcatattt ccaattaatc
    74281 acaaattcac ggcattacta gaaaaattaa aggggcgcag aagtggaaaa atactcttcg
    74341 actttcccta caaaccaggc catcttgtgt ttaataacta taccaaatct aaggtacctc
    74401 taaatctaaa ttccatttgt ggcggtgacc tccaattttt aactcttcta tctcccatcc
    74461 agtatacata accttgatac ttcgaccact tatatatcga acacacaaat acctatccat
    74521 tgcaggagta ctacctaact tagttgctac ttgcatctaa ataacagctg aaaagatagg
    74581 acttactctt agtaaagtat ctgaactatt aatttttaaa ctatcatttt tatcctcaac
    74641 tacatgcaca tctgttgcaa cttcactttc tgtctcatcc agataaatga ttaaggaatc
    74701 gggaattgca atatcttcag ctgacgctgg aatagttgag ctaaattagt catcatatct
    74761 ttattataaa ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat
    74821 cattaataaa tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca
    74881 acatataccc tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc
    74941 atagacactt ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa
    75001 aaagtgactc aaaataaaaa aaaccttaca ttatactgga atgaact
//
LOCUS       DACEDD010000007        74596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEDD010000007 DACEDD010000000
VERSION     DACEDD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.007"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(460..1779)
                     /locus_tag="IWO45_000527"
     CDS             complement(460..1779)
                     /locus_tag="IWO45_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0332029.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            2036..2506
                     /locus_tag="IWO45_000528"
     CDS             2036..2506
                     /locus_tag="IWO45_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0332030.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2728..3831
                     /locus_tag="IWO45_000529"
     CDS             2728..3831
                     /locus_tag="IWO45_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0332031.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            4052..5011
                     /locus_tag="IWO45_000530"
     CDS             4052..5011
                     /locus_tag="IWO45_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0332032.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            5181..5861
                     /locus_tag="IWO45_000531"
     CDS             5181..5861
                     /locus_tag="IWO45_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0332033.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            5951..6985
                     /locus_tag="IWO45_000532"
     CDS             5951..6985
                     /locus_tag="IWO45_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0332034.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            6999..7334
                     /locus_tag="IWO45_000533"
     CDS             6999..7334
                     /locus_tag="IWO45_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332035.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            7318..9798
                     /locus_tag="IWO45_000534"
     CDS             7318..9798
                     /locus_tag="IWO45_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332036.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            9854..11413
                     /locus_tag="IWO45_000535"
     CDS             9854..11413
                     /locus_tag="IWO45_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332037.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            11410..12774
                     /locus_tag="IWO45_000536"
     CDS             11410..12774
                     /locus_tag="IWO45_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0332038.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            12945..14015
                     /locus_tag="IWO45_000537"
     CDS             12945..14015
                     /locus_tag="IWO45_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332039.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            14030..14698
                     /locus_tag="IWO45_000538"
     CDS             14030..14698
                     /locus_tag="IWO45_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332040.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            14718..15296
                     /locus_tag="IWO45_000539"
     CDS             14718..15296
                     /locus_tag="IWO45_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0332041.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            15697..16608
                     /locus_tag="IWO45_000540"
     CDS             15697..16608
                     /locus_tag="IWO45_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0332042.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(16986..17903)
                     /locus_tag="IWO45_000541"
     CDS             complement(16986..17903)
                     /locus_tag="IWO45_000541"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0332043.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            18105..18542
                     /gene="zapA"
                     /locus_tag="IWO45_000542"
     CDS             18105..18542
                     /gene="zapA"
                     /locus_tag="IWO45_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0332044.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            18600..19148
                     /locus_tag="IWO45_000543"
     CDS             18600..19148
                     /locus_tag="IWO45_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0332045.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            19263..21623
                     /locus_tag="IWO45_000544"
     CDS             19263..21623
                     /locus_tag="IWO45_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0332046.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            21710..22024
                     /gene="trxA"
                     /locus_tag="IWO45_000545"
     CDS             21710..22024
                     /gene="trxA"
                     /locus_tag="IWO45_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0332047.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            22206..23984
                     /gene="uvrC"
                     /locus_tag="IWO45_000546"
     CDS             22206..23984
                     /gene="uvrC"
                     /locus_tag="IWO45_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0332048.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            24129..25028
                     /locus_tag="IWO45_000547"
     CDS             24129..25028
                     /locus_tag="IWO45_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332049.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            25021..26283
                     /locus_tag="IWO45_000548"
     CDS             25021..26283
                     /locus_tag="IWO45_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332050.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            26444..28342
                     /locus_tag="IWO45_000549"
     CDS             26444..28342
                     /locus_tag="IWO45_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0332051.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(28672..29391)
                     /locus_tag="IWO45_000550"
     CDS             complement(28672..29391)
                     /locus_tag="IWO45_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0332052.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            29728..30060
                     /locus_tag="IWO45_000551"
     CDS             29728..30060
                     /locus_tag="IWO45_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332053.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            30047..32050
                     /locus_tag="IWO45_000552"
     CDS             30047..32050
                     /locus_tag="IWO45_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0332054.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            32069..32539
                     /locus_tag="IWO45_000553"
     CDS             32069..32539
                     /locus_tag="IWO45_000553"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0332055.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            32560..33144
                     /locus_tag="IWO45_000554"
     CDS             32560..33144
                     /locus_tag="IWO45_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0332056.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            33159..34145
                     /locus_tag="IWO45_000555"
     CDS             33159..34145
                     /locus_tag="IWO45_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0332057.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            34135..34446
                     /locus_tag="IWO45_000556"
     CDS             34135..34446
                     /locus_tag="IWO45_000556"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0332058.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            34468..36249
                     /locus_tag="IWO45_000557"
     CDS             34468..36249
                     /locus_tag="IWO45_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0332059.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            36242..37618
                     /locus_tag="IWO45_000558"
     CDS             36242..37618
                     /locus_tag="IWO45_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0332060.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            37625..38260
                     /locus_tag="IWO45_000559"
     CDS             37625..38260
                     /locus_tag="IWO45_000559"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0332061.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            38290..39645
                     /locus_tag="IWO45_000560"
     CDS             38290..39645
                     /locus_tag="IWO45_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0332062.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(39837..41231)
                     /locus_tag="IWO45_000561"
     CDS             complement(39837..41231)
                     /locus_tag="IWO45_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0332063.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            41323..42294
                     /locus_tag="IWO45_000562"
     CDS             41323..42294
                     /locus_tag="IWO45_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0332064.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            42291..43073
                     /locus_tag="IWO45_000563"
     CDS             42291..43073
                     /locus_tag="IWO45_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0332065.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(43242..43613)
                     /locus_tag="IWO45_000564"
     CDS             complement(43242..43613)
                     /locus_tag="IWO45_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0332066.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            43747..44190
                     /locus_tag="IWO45_000565"
     CDS             43747..44190
                     /locus_tag="IWO45_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0332067.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(44233..45042)
                     /gene="yidA"
                     /locus_tag="IWO45_000566"
     CDS             complement(44233..45042)
                     /gene="yidA"
                     /locus_tag="IWO45_000566"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0332068.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(45061..46428)
                     /locus_tag="IWO45_000567"
     CDS             complement(45061..46428)
                     /locus_tag="IWO45_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0332069.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(46412..47242)
                     /locus_tag="IWO45_000568"
     CDS             complement(46412..47242)
                     /locus_tag="IWO45_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0332070.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            47405..47842
                     /locus_tag="IWO45_000569"
     CDS             47405..47842
                     /locus_tag="IWO45_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0332071.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            47918..48538
                     /locus_tag="IWO45_000570"
     CDS             47918..48538
                     /locus_tag="IWO45_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0332072.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            48727..49233
                     /locus_tag="IWO45_000571"
     CDS             48727..49233
                     /locus_tag="IWO45_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0332073.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            49250..50149
                     /locus_tag="IWO45_000572"
     CDS             49250..50149
                     /locus_tag="IWO45_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0332074.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            50236..51120
                     /locus_tag="IWO45_000573"
     CDS             50236..51120
                     /locus_tag="IWO45_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0332075.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(51312..51383)
                     /locus_tag="IWO45_000574"
     tRNA            complement(51312..51383)
                     /locus_tag="IWO45_000574"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51348..51350),aa:Arg,seq:ccg)
     gene            51443..52777
                     /gene="rpoN"
                     /locus_tag="IWO45_000575"
     CDS             51443..52777
                     /gene="rpoN"
                     /locus_tag="IWO45_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0332076.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            52980..54017
                     /locus_tag="IWO45_000576"
     CDS             52980..54017
                     /locus_tag="IWO45_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0332077.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            54058..55059
                     /gene="gap"
                     /locus_tag="IWO45_000577"
     CDS             54058..55059
                     /gene="gap"
                     /locus_tag="IWO45_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0332078.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            55169..56359
                     /locus_tag="IWO45_000578"
     CDS             55169..56359
                     /locus_tag="IWO45_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0332079.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            56463..57218
                     /locus_tag="IWO45_000579"
     CDS             56463..57218
                     /locus_tag="IWO45_000579"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0332080.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            57334..58632
                     /gene="eno"
                     /locus_tag="IWO45_000580"
     CDS             57334..58632
                     /gene="eno"
                     /locus_tag="IWO45_000580"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0332081.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            59032..60171
                     /locus_tag="IWO45_000581"
     CDS             59032..60171
                     /locus_tag="IWO45_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0332082.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(60238..60813)
                     /locus_tag="IWO45_000582"
     CDS             complement(60238..60813)
                     /locus_tag="IWO45_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0332083.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(60818..61792)
                     /locus_tag="IWO45_000583"
     CDS             complement(60818..61792)
                     /locus_tag="IWO45_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0332084.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(61816..61860)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            62075..63043
                     /locus_tag="IWO45_000584"
     CDS             62075..63043
                     /locus_tag="IWO45_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0332085.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            63156..63434
                     /locus_tag="IWO45_000585"
     CDS             63156..63434
                     /locus_tag="IWO45_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0332086.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            63552..63788
                     /gene="secG"
                     /locus_tag="IWO45_000586"
     CDS             63552..63788
                     /gene="secG"
                     /locus_tag="IWO45_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0332087.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            63900..64646
                     /locus_tag="IWO45_000587"
     CDS             63900..64646
                     /locus_tag="IWO45_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0332088.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            64649..67006
                     /gene="rnr"
                     /locus_tag="IWO45_000588"
     CDS             64649..67006
                     /gene="rnr"
                     /locus_tag="IWO45_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0332089.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            67028..67492
                     /gene="smpB"
                     /locus_tag="IWO45_000589"
     CDS             67028..67492
                     /gene="smpB"
                     /locus_tag="IWO45_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0332090.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            67794..68513
                     /gene="atpB"
                     /locus_tag="IWO45_000590"
     CDS             67794..68513
                     /gene="atpB"
                     /locus_tag="IWO45_000590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0332091.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            68566..68781
                     /gene="atpE"
                     /locus_tag="IWO45_000591"
     CDS             68566..68781
                     /gene="atpE"
                     /locus_tag="IWO45_000591"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0332092.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            68872..69396
                     /gene="atpF"
                     /locus_tag="IWO45_000592"
     CDS             68872..69396
                     /gene="atpF"
                     /locus_tag="IWO45_000592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0332093.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            69383..69925
                     /locus_tag="IWO45_000593"
     CDS             69383..69925
                     /locus_tag="IWO45_000593"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0332094.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            69948..71504
                     /locus_tag="IWO45_000594"
     CDS             69948..71504
                     /locus_tag="IWO45_000594"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0332095.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            71518..72420
                     /locus_tag="IWO45_000595"
     CDS             71518..72420
                     /locus_tag="IWO45_000595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0332096.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            72443..73849
                     /gene="atpD"
                     /locus_tag="IWO45_000596"
     CDS             72443..73849
                     /gene="atpD"
                     /locus_tag="IWO45_000596"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0332097.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            73863..74285
                     /locus_tag="IWO45_000597"
     CDS             73863..74285
                     /locus_tag="IWO45_000597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0332098.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        24755 a        13076 c        15810 g        20955 t
ORIGIN      
        1 gcaaaacatt tttaacgtaa gcgccccata gaacagtacc ttactaacaa atgcacctca
       61 agatatactt gagctatcaa gaagtctgga ggtcttctat gtataaatta aatgagattg
      121 ttcctgtcca attaggaaac gaaaagagtg caccagaaaa aggtaaaccc gcggctgcca
      181 agttagcgat gcgcatcaaa agagagaatg gttctgaagt tttgatctat aacggtatta
      241 acaattatat tcttcacctg aataattcat agctttaatt ctttggcaca ttttattgaa
      301 atatgtatca ctaatatccc tatcaactga ttttgactaa aaagttactt caaacattca
      361 ctgttcaaaa atattgagga ataaattttg ggtgtaaggc attttgagca tacttctccc
      421 tggataactt cccagtaaaa aaatcgttag attgttggat tagatccatt gactggctcg
      481 cctcaggcga gcaaagaccc tgtagaattc attctgatac acatgataac tagataattt
      541 gagatagact cgtctacccg tttggactaa tttcccagca actttcagaa acttcaatcg
      601 aatagaatga atggtcattc ccttttggac ttcttcaaag ccaatcgttc ttaagaagtt
      661 gactaagttg taagctatca agctgagcat cattcggaca tgattctcca aaaagcgagg
      721 actgtctgtt ttgtcaaagt aaaagccagc tttcgcttct ttaatgaagt tctccattgt
      781 gccacgtttg gcatagatag aaaagatggt atcaggagaa acattttctg atagattcgt
      841 cacgataaat tcatgtcgaa agagtagttc gcccgcttca cgtgttgagc gtatacacac
      901 gcgacgactt tgtgaccagg attgtgcttg ataagtggtc gagaagtact gaacttctcg
      961 ttcttcccac ttttgattat cgccataaag tactgatttc tcagctattt gacctagtct
     1021 acgattattc ttcagtcgaa taacataatg actctttttt gattcacacg aatcatacac
     1081 accaggtgta gcgaacccgc tgtctccacg aaccaagatg tcagtgtttg gtagagagtg
     1141 attatagtgc tctaataaag gtgtgagaaa ctcctttacg ccttttgatg tatattgatt
     1201 tcctgaacgt agttcagctt ttaagaagtc accagtcaat ccgtcaaaag ctaccaatgg
     1261 atggtaacca taggtttggt agtgtgtatt ataattcgtt tgttcttggt gaccaaatgt
     1321 atctgaatgg gtcgaatcta aatcaatgat taattccgta tcattacgga tgaggcgtgc
     1381 tttatcgata agttcttggt tcaaagcttg aagttcatgg atattctcct cagatagtcg
     1441 atctaaaaat cgggaaagtg aagattgaga agcgagttct ttcctatcta aaacagcttt
     1501 aaacacagga tcttgtctca ggagattagc tgcagaatca gctgagtaac cagcaattaa
     1561 ttgcataatg aactgctcaa gtatggataa gttgtcatga gtaaaatacg ctcgttcgtc
     1621 ttcaatgtga atgtgttgct tagccagttc agaaaaacca aagatgttca tcagctcttt
     1681 cactaggacg agacccgaat cactcgataa ttgaccacct gtatgagaaa tagtgatatt
     1741 tgaattgaat tttacttggt ttttgtgtaa gctagtcatg aaaagaactc ctttcttttg
     1801 gttggtttag tcactttaac catagcagaa aggggttctt ttttcatcac ttaacgggtg
     1861 aagatgaaaa agtaattgta agctgccgtg aggcagtttc acatggtttt aattaaaagt
     1921 atgaattatt caggtagaat ctctgaagaa taaatcacgt gatcgtgacg ataaaataca
     1981 cttacgtcag gtgataaaaa atacataagt agaaaactaa gaggaggaac agaaaatgaa
     2041 agaagttcaa ttgatcagaa aaagcgaact atcagaaggt ggttgcaatg catgcggcgt
     2101 agtagaagca acaagctata cattaaagct tggtgccaac aaagcgataa tctctgagtt
     2161 gacagtaggc ggtcttgtgg acagtctggc gcttgctgaa ggttttattg gagaagatat
     2221 ttatgagatg ttttcagaaa tccgtcagtt aaaaaaaggg gaaaactgca tagaggttca
     2281 tcatgagagc ccgaatgtga gattcaaacg tggtgataat gagatgatct tcaataatca
     2341 tgtgtccgat cacaccgagc tatatggaat cgtgaatcaa atactaactg agctttttgg
     2401 attgggacct tatgcgttca aggaagaaaa cggtaatcca aaattaaacg aagaatggca
     2461 agaaacgatt gaaatacaaa gaaacaaccc gcacttattt caataggcat ccacagtatt
     2521 tcgtaaaagg gactttgaca cgacttttgt caaaatccca tcgtttttta tctatttgtg
     2581 ttctaaacat ttctttcaga gtagtaacaa ctggaatgac tgaaatgtaa acagattatt
     2641 tgcctgtatc aatacggaag agtaatataa agtgtgtaaa cgctatcata ttattcattc
     2701 aattttttat agagggaagg cattgaaatg gaaaaaacgt atcaagcagg tacaaatgaa
     2761 ggaatagtgg attttatcaa tatggaagat ttagaaatag ccgcttcaca agtgattcca
     2821 gctggtggtt atggttacat cagcagtggt gctggcgact tatttactta tcaagaaaat
     2881 gaacgggcat tcaatcatca gctgattatt cctcatgtat taagagatgt agaattaccc
     2941 gatacaacta cacattttga caaagaaacg ctgactgcac caatcatcat ggcaccagtt
     3001 gctgctcatg gcttagctca tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat
     3061 tttgggacga tctatacagc aagttcttac gcttcttgta cattagaaga gataagagaa
     3121 gcaggcgggg agaaagcacc gcaatggttt caattttata tgagcaaaga tgatgggatc
     3181 aatttggata ttcttgaagt ggccaagcgt aatggagcaa aagcaatcgt attgacagcg
     3241 gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa atgggtttac cttcccactg
     3301 ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc aaacgatgga tgctgtgtac
     3361 aaatcttcca aacagaagct tagtccaaaa gatgtagaat ttattgctgc tcattctgat
     3421 ttgccagtct atgtgaaagg tgtccagtca gaagaagatg tctatcgttc attagaatct
     3481 ggtgctggcg gaatctgggt atctaatcat ggtggccgtc aactagatgg tggtccagca
     3541 gcgtttgatt cgttgcaata tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt
     3601 gacagcggtg tacgacgcgg acagcatgtt ttcaaagcaa tcgcatcagg tgcagatctt
     3661 gttgctattg gcagacccgt tatttatgga ttgtctttag gaggaagtac aggagtccat
     3721 caagtgtttg atttctttaa aacagaattg gaaatggtca tgcaattagc tggaacacaa
     3781 acagtagaag atatcaaaaa aataaaattg cgtgagaatc gttttattta agaaacaaat
     3841 gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc tatttttata gttcaaggat
     3901 gtcactaata gaataatttg aaacgaaaaa ataaaattga aatatttttt catacttcgc
     3961 cgattgtaaa attaagctag acaactaaaa aaagtcattt gtgctacact caaaatagtt
     4021 ccgacgaaag aattataagg agtgagtaca aatgacctat aaccatctta caccaactga
     4081 actcgtaatg atagaagctt atttcaatca atcacaacct gtttcaaaag tagccaatct
     4141 gttacaacgt tctcgacaaa ctatttataa ggtctatcgt tttcttaaat ctggtggaag
     4201 cgcaatgagc tactataaac aatataagaa aaacaaacgt aactgcggaa gacatccgat
     4261 tgttttacct gatgagcaac aggaatacat tcaaaaaaag gttgttcaag ggtgggctcc
     4321 tgatgtcctt attggtcgag cagagtttcc tattgcttgt tcggtacgca cactgtatcg
     4381 aatgtttaaa gataaaacct ttgacactca cgatttacca atgaagggaa aaagaaaacc
     4441 aaacggtcat aaagaaaaaa gaggaaaaca agggtttcgc cggtcaatcc gagacagaaa
     4501 gacagactat acccagtttg atcatgaatt tggtcacctg gaaggtgaca ctatcgtggg
     4561 tggaaaacat aaaagcgcgg tcatcacatt agttgaacgg ctatctaaag tcatcattac
     4621 cttgaaaccc gaaggcagac aggctaaaga cattgaaaat aggctgaatc aatggtttcg
     4681 ttgtgtccca aaaaacttat ttaaatccat cacatttgat tgtggaaaag aattctcaaa
     4741 ttggaaaaag attagtaata ccaacgatat ttcaatttat tttgcggatc caggaacacc
     4801 ttctcaacga ggattgaatg aacattcaaa tggcttatta cgtaaagatg gtttgtataa
     4861 agctatggat tttaatggtg tatctgaaac ctttatccaa tcggtggcat cgaaacgaaa
     4921 tcatattcct agaaaatcat tacactatcg gacaccaatg gaagtatttt tgagttacgt
     4981 cacaaaagat gatttgtcta acttaatttg acaaacgaaa cttaataaaa aaggaagaat
     5041 attcatgtaa acgattttat tgtttttttc tcttttatac aaaaagggtc ataatttcaa
     5101 gtaagcggat gaattcatga ggattttttg catactgtct ctactatact taggtcacta
     5161 agaaaaagga gtgatttatt atgaaaaaga acatgattgg gacattatta ctttcaacac
     5221 ttcttctagg cggcttggca acaccagctt ttgctgaggg tcaagcgact tcaaaaggag
     5281 atattacctt tactgaacca acaaataccg tggagcctct taaccctact gatccttcaa
     5341 aaccagtaga gccagcagat ccggaaaatc cagcaactgg ccagacaggt cctttaacac
     5401 tggatgtagt tcccgaattg ccatttggta cacacgagat tgaaagcgga acaaaaacgt
     5461 atcaagtaga cgcttcaaaa aacgatacac cttatctcca agtatcagat cgtcgaggtg
     5521 taggtgcaga tggccaagca caagggtgga acgtgacagt ttcggtttca gattttgtaa
     5581 acggttcaca agttttgcaa ggcgctgaat tagattttgg aacatccact gttaaaagta
     5641 cgtcagataa cgaatccact ggaccaacta gtcaaactgt aactgggttg agtaaagcaa
     5701 gtgcagcgac accaatattt acagcagcaa aagatcaagg tctgggaaca tggctttctg
     5761 tttatgatcc agcaaatatc acactgaaag tgccgaaagc agcagctgga acattcacgg
     5821 ctgatttgac ttggaacctc gtggctggcc cagtagcata gagtatgaaa taaattcaac
     5881 aaactgcggt gaaaatacta taggtgaagg attttttacc gcagttgttg ttgaaaggat
     5941 gtggtggaca atgaatcaaa gtaaacgact gtttgtttcg tttttttcta ttttaagtct
     6001 atttttcatt tttccgtcaa tcagccaagc aaaggattca gcaggagact ttggaatcaa
     6061 accggtattt ccagagaacc aaatagataa agcaatcggt tattttgatt tattagtggc
     6121 accggaacaa aatcagacac tagaagtaat tatcagcaat tcttcagatg aagaacgaac
     6181 atttgaggtc tctgtcaatc cggcggtaac gagtgatggt ggaacgatcg attatagtca
     6241 aaaaaaccca acgttagatg agacattgcc gtttgatgtc cgtgacgttt tgctcattgc
     6301 gaaaaaggaa atcaatgtat cagcacatgc agaaacaact gtacctatag aagtgaaaat
     6361 cccagcaaaa agctttaagg gacgtgtatt agcgggtatt catgtttcac ctaaagaaga
     6421 agctgaaaca gaaaatgcta cagaaggagc acaaatcaaa aatcgtattg cttataatct
     6481 cgcagtcgtt ttacaagaaa gtcaagagac gatagaaccg gatctgaaac ttttatcagg
     6541 agatttggac gaggtcaacg caaagccaac tgttcagctt cgtttccaaa atcctcagcc
     6601 taggattatc agcaatttga tatttacgag caagatcttt tatgaaaatc agctttatat
     6661 tgaaaatacg tctaatgcat ttttagttgc accaaattcg aattttcact tgaatcttga
     6721 tttagctggt gataaagcaa aagccggcga ttaccgcgca gaaatcatag caaaatcagg
     6781 cgacagtaat gaatggcgat ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa
     6841 agtaaatgaa aattccgttt ttgcagtaca agaacaatct ttcccatggc tttatgcagg
     6901 attggttgct gcagttctag tgttatggtt gattttgctg tttttgtggg tccacaagaa
     6961 aaagaaaaaa ggagaaacaa catgatgagg tgatttcgtt gaaaacatcg atcttattgg
     7021 gagggattat tgcaagtttg ctgttcttca gctggcaaga gccagtccaa gcagatacca
     7081 aaagttcagg tacgattaca ttttatgatc taagtccaat tccgcatcca agtggttcag
     7141 tcaatggcgc atcaaaacag cagacacagc taccgattta taaagaatct aatcaacatg
     7201 gcgtattccc ggagacgaat gaacaaaaga tccgttttct ttatttgatc attggtatag
     7261 aattatggct tcttgcgtgg ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg
     7321 ttgctacaaa ataaattttc attttttcgc tccttctttt ttacttcgct ttttgtttta
     7381 agtatattta tcttttcacc ttcggcagaa gctgttggag caacgcctcc tgccatttct
     7441 tatgatccat acacgtatga aaatatcgta cctccaaatg gtggacgtag tgcagtcaat
     7501 ggcgggaagc ttgtccaact aactggggct aaccaattgc ctaaattgac tgctacttat
     7561 ttgaacgatg gttcaaatga tccttggaaa ggtggcacgg tccgttttca ttgggaaagc
     7621 catgtgaaac taacaaatgt acttgatttg gtgaatatca ctgcacaagg atttgcattt
     7681 gtggcgatca tcaatttgcc ggaacatgta tcaagcagtg atgtattaaa agcaattgac
     7741 tggaacaacg cctacttgta tattgccggt gcccccatca aggttactag cggaagttta
     7801 ttatcagcag gaagccatac cttgcgtttt gggatggggt cgtgggatac aaatactcta
     7861 ttgacagata ttattgctat tattacgcga ggcgaattca acgtggacga tatcccgctt
     7921 caatttgatg ttacagctaa tgtcgcgaag atgacagaaa acggagataa atatgatacg
     7981 agtccaaaca aaattttgac caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt
     8041 ttttctgttg atttttatga ttcgaataat attatccaag atcgtatgtt caattcgatt
     8101 ttagctccag ctgcaggtag attttatcct aaattaactg gcggaaactt gaatccagga
     8161 gcatatgcat tttctgcaag tgcagagata aatacgtgga atagttacat ctcaccaagt
     8221 gattcaacag gaaaatataa tacatttgct gataccacag aatacgtgga tggaaataac
     8281 cagaaccttc caggctctac caatagtcgg acattgacga tggggctgac tgaaggacgt
     8341 tttgatcagt tgccatttag tcgctttgat cgcgtagtca actattttac aggaaaaaac
     8401 gttacacaag gagcaatact gactcatact ccagcaagca caagtggatt gaatcagaca
     8461 gccaatatcg tgtacagtgg gaaagatgcg caaggcggat tgctttctcc tgttgcttta
     8521 aaggtattgg agcagtacaa gctagacgga acggttttac caacaaactt aacagccgat
     8581 agtcagtggg gaaagctcct ttcttctgca tcgtaccaag tcacggccaa ttggtcagca
     8641 actgaactaa aaaagggttc gatccattac aggctttatt cgaaaggaac ccaaaaatta
     8701 gcgacaggat ggacagatca acttttccaa acgatccaat caaataacgg gggtgtaaat
     8761 acagctactt ctacactacc tgcattggct tcagggcaat attattttga ttatcgcttg
     8821 gtggacgatg tacttactaa catgtatccg tcttttgcgt acaaatggca gtctgaacaa
     8881 gctggaatca ccactcttcc tcaaataaca gtggctaatt ttccatctgt gagtgcagat
     8941 agtcagctaa aaaaccttag cagacaacct gagacaggag atcccctgtc tgctctatca
     9001 ggagatacga ttcaagaaaa tgtgacattc acgttaacaa aatctgggga tgcgataacc
     9061 gacaaaaaaa tcacgatctc acttcctgaa aatactactt atatcaatgg ttccttaaaa
     9121 ttaaatggta caactatcgc agataccggc attcaacaag gagtttctgt cccagcagat
     9181 ctattgagca aaataggaga taccattcat cttacttaca actatcaatt aaatactgta
     9241 gatacttctt tacaatccgt cagcatactg acaaaggcag ctgtactttc cagcaatatt
     9301 acattagcag atggtgctaa gcttcctaac ccagttgtcc aaacatctgc taaaacaatt
     9361 cttgtcccca aacaagaatt aacattagtt aatgtaccag atgattttac atttggtaac
     9421 gatctcccca aaccgttgaa gacaagttat tatgaatcaa aaggcgattt ttcttttgat
     9481 gtgcgggata ctcggcttcc aagcacgtct ccttggcaac tgacaggtac attgacttct
     9541 ttatttaaga atgatcaagg tcaagaatta agcggaacaa aattatactt caatcattct
     9601 ggatcaaagc aattgattca gcaaggtcaa aacacgttga tttatgagag tgatggaaca
     9661 gctaaaggag aggttctcgt tgactttccc gataccgatg gtttactctt agaggtaaat
     9721 agcagtacga atgctcaacc tggcgcaacg tatcaaggca tggtcacatg ggaactgaca
     9781 gctgggccaa ccagctaagt gattagaatt tgtatggatc tgtgataact gtcacagatc
     9841 ctctttttca aggatgagaa ggaaacttct gctgttaaaa tcagaaatgc ttgaaggagt
     9901 ggacttggaa cttttcagtt tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca
     9961 acttttatta gaggaaaaag gagaggtttc cgttaataac gtacttaccc gcttatcagt
    10021 tgatcgggct accttgaaag aagatctgtt ttatttacaa gctaacttgg aaaatatcaa
    10081 acaattggaa attgttatcc aagaagggaa tattttatta atcagacatg gaaatatagc
    10141 agcagtcgat gtttattatc attgttactt gcgctattct acaaaatatc agatacttct
    10201 ttatctgttg gaaaatggct catttgatcg gcagaaactt gtcatggaac ttaatatcag
    10261 ttcggctact ttgtctcgca gagtgaagga attaaatgat tctttaaatg agtttcatct
    10321 gaaaataaaa agcgggcggc tgaccggtcc agaaagtcaa attcgttatt tttatttcca
    10381 attattttgg tttggacgtc cctatcttga aaacaaaaaa gaatacagtg atttatcagg
    10441 aaacactttt ttagatctgc tgcaaaaaga gtttagtttt gctattacag aagaaggacg
    10501 attgaaattt gaaatatggc tttccattat gaagaagcga atgcgctgta cgaaaaaatc
    10561 caaaccagat tatagcaatc ttcctgttgg attgtttcaa acgacacaat ttttattgga
    10621 cctccataca tttttgaccc gttacttctt tcaatatgcc tttccttgga atcaaaatga
    10681 aacgttggtt ttttatcttt tcatgatcag taactttaca ttagatacac ataactcgca
    10741 tgtccaggag tttttgataa gcgaaaaata caaagacgac gtagtagctc aattgaatcg
    10801 aacgtttaaa caatcgatag aagaatattt ctctttttat gtttttccag aagagatttc
    10861 gcaaaagatt gcgttgacag tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg
    10921 gatgaatatt tttggccaag aaggattaaa acagcggtta actgaagtag aagataaaca
    10981 attcctggtt ttatgtgaaa gattggtcag acaaacattt gctattgttg attttactga
    11041 agtagacgag gaaaatgccc gctttgaatt atttgctcgc tatgcatcta ttttacggac
    11101 gctgatcaag cagttagaat tacgtctgca aattgcttat gatcttcctt atgaaaggct
    11161 aatggcggat atcgtgaaag aaatcatcca ggaacaattg ctgaatacga tcaaatatga
    11221 tttagtcgaa tacgagaaag aaaagcagta tgatatcatt ttgactagcc aattgaaaga
    11281 gtacccgtca cagaagtccg ctaaagtttt tgtattcact agcaacgaat ataattatga
    11341 tttcccctat ttgaaccaat ttttaaaaga atgttattta gaaaaattga atttaaggat
    11401 gaataaaaca tgaaaatcgt gatcattggt gcttcccatg aaggcatcac agctgcccta
    11461 aatctaagaa aacttcaacc gaaagcagaa gttttactga tcgatgaaga tcataaagat
    11521 ggactaggct atgtttcaaa tggaatcaac ctttatttaa aaggaaagat acgtagttta
    11581 gctgaagttg cccataacat gcggactctt caagaaagtg gggcaaaact gatcaccgaa
    11641 tggagagtaa ctgaacttga tccagacaaa catcagctca tcctcgcatc caaagaagga
    11701 aaaaacgaga ccataaccta tgataaatta atcgtagcga caggttcctc tcctgtcacc
    11761 ttgtataaac aaattgaagc tgaaaatgtc tacacctata aaaatctagt gcaatctaag
    11821 caagtacttg ccgctttaaa agaagcaaaa gaagtagtga tcttcggtgc tggttacatt
    11881 ggattggaat tagcagatgc tttgagaaat aaaggataca ttattcatct agttgattat
    11941 atgccgaatg ttttatcaag atatttcgat aaggatatga tcaacagttt tcaaaatcag
    12001 ctgcaaacta agcaaataaa tttttatcca aatgaattct tgatcgattg gaaaaaatca
    12061 gaagagaaag tagtatccgt acaacttctg tctcaaacaa taaaggcaga tatggtcatt
    12121 ttttctgccc aaactcgtcc taatacgaca ttgttaaaag aaaaagtggc tttgtatgag
    12181 gatgagactg tgatagtcaa tgaatatctg cagacaagtg atccggacat ctatgcgatt
    12241 ggagatatcg tcccagtcag ttttgacaaa aataaacggc atctcttttt acctttagtt
    12301 actcgagctg tacatatggc aagagcagta gcgctaactt tgagtggtca gccaaccgct
    12361 tacgacctca gacaaaagat aacagcagca gtcattatag actacttttt aggaactgta
    12421 gggctgacag aagatgaagc gccattcttg gaacaaaata ccggttcgtg ttcaggagaa
    12481 tttgatctat ttccacaata cgacgaagaa aacaaaacag taaatgcaaa gctgatctac
    12541 catccggata cattagaaat aattggcgga cagctgataa gccaagaatt cttattatca
    12601 gatttgaacc tgcttgcgga tatcgtgaaa cacaaaacta cgattccaca gctagcagtt
    12661 gaggattttg gatttctgtc tgaatacacc tctcgttttc actacctcaa tgagttagct
    12721 tttaaagtac tagctaagag agcaaatagc aatagagtag ataaaagact gtgagaaata
    12781 tcacggtctt ttattgtgtg gggatataat aaatattaat caatcgagaa taaaaaattc
    12841 atataatgaa atagcggaag aattttttga gcataatatt cgccaaaaat ttatcaagtg
    12901 ataaaattgt tgtataaaat gattatttca ggaggaggag ttaaatgttt ttagcattaa
    12961 atgaaattat gcattcaaag ttacgttatg cgttagttgc aggggtgatg tttttgatcg
    13021 cctacctggt attttttttg actgggttag cttatggctt agctcaagat aatcgaacag
    13081 cagtcgacaa gtgggaggca gattcgattg tgctttcgaa agatgcaaat tcaaatctag
    13141 ggatgtccat gattactaag aagatagcag aagaagtcga aggtgggaaa gtggcgtatc
    13201 ttgcccaaac acctggagtc gtgactagta aagacagtac agaagaagga aaaatcaatg
    13261 ttagcttctt cggtatcgat aaaaatcaat tcatcatgcc caatcttgtt gaaggaaaag
    13321 cttttgataa cgatgatgag gcagtaggcg atattagttt gaaagaagaa tatggtctag
    13381 ccataggaga tacagttaag ttatctggta gtgacaagac gtttaaacta actggcttta
    13441 cggatcatgc caaattcaat gtctcaccag ttctctatac aaccatcaat gcttaccaag
    13501 aaatccgctt cgaaaaagaa gatacaagtg aaaatgcacg gattaacgca atagttgtca
    13561 gaggtaaaat cagtaattta cctgaagatt tagaacagat caaaatttct aaattcatca
    13621 atgaattacc tggatataat gcgcaagtat tgacttttgg atttatgatc ggatttttga
    13681 tcgttattgc ggctattgtg atcgggattt tcatttatgt actgacgatg caaaagatta
    13741 atatctttgg tgtaatgaaa gcacaaggga tcacaggagg atttattgcg cgttctgtag
    13801 tggcacaaac attcatttta tcatttgttg gtattcttct tggtctatta ggaacagtgg
    13861 gaacatccct tgttctacct gatgcggttc cattccaaag caattggctg ttttttggcg
    13921 tcattagctt gttgatgtta gtagtagctg tgttaggcgc actattttct gttcggacaa
    13981 tcgtaaaaat cgatccgttg aaggcaattg gataagaagg aggaagaaca tgaaagcaat
    14041 agaatttaaa gaagtagaaa aaagtttcca agatggcgac caagtagtta aagcattgaa
    14101 agaaacaacc atgagtatcg ataaagggga atttgttgca atcatcggtc cttctggttc
    14161 aggtaaaagt acttttttga ctttggcagg cggattgcag acgcctagca gtggctctgt
    14221 atttatcaat gatcaagaat tcagtaatca aaaagaaagc gaacgcgtta aactacgttt
    14281 taaagaaatt ggctttattt tgcaagcttc caatctggtt ccttttctga cagtaaaaaa
    14341 gcagttatta ttagtagata agattaaaca tgaacatcgt caaaaagaag caaacgaatt
    14401 atttgagcag cttggcgtgg ataagttgat caacaagtat ccagaagaac tatctggtgg
    14461 agaacggcaa agggtcgcaa tcgctcgtgc attgtacaat gatccgtcca ttattctagc
    14521 agatgaacca actgctagtt tggattcaga aaaggcacga gaagtagtcg atatcttggc
    14581 aaaagaatca aaagacaaaa ataaagcaac gatcatggtt actcacgata cacggctgat
    14641 tgacaaatgc gatagaatat ttatgatgga agatggtgta ttgaaagaaa aagaataacc
    14701 aaaaagcagg tggaaaaatg gtccatcgat acaacaaaga tacacagcaa aaaattttga
    14761 aagaagcgac tgacttgttt atgtccaagg gatatctcgg cacttcaaca agagaaatcg
    14821 cacaaaaagt tgggatcacc cagcccaatc tctatcatta tttcggtgat aaagaaaaac
    14881 tatatacggc agttttagaa aaccacttaa aagatgttgg aaaagctttg agagaaatcg
    14941 ttcaaacaag cgaaacggga tttcaagtaa ccttgacaaa aatggcgcag tttttgattg
    15001 agacacatct ggtcgatttg tttctgatgc ttcacgatct tgaaagcaat ctttcaaagg
    15061 aaacaagaga tcatcttttc tttctatgga agaaaaatta tcgtgaacct tttgaagaaa
    15121 tcttttcagc aaaccaatct gtgctaagaa atgggatttc aaaagaaatc gcagcaaggc
    15181 attttttctt agtgttagca ccttacatca ctcggtcgaa aagacctgtg gaagaaccgc
    15241 taaatgtggc gcaattgatc gatttatatt tgaatggtgt attaggagaa gcgtaaagct
    15301 gttatatagg gagaaaatag ttaggtgaag aagcaagata tgacgaagat cgttatatct
    15361 tgcttcttta tataggtagt ttttcataaa tacaaatttg ttattctctc atgattatca
    15421 gggtatattt ttatttatta tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga
    15481 aattaattgt ttgtagaatg aaggaaacta tttattgggt agatgaatca ccacgaatag
    15541 tcaaaaggag atttcaggat atgccaaaaa tgaaaaaaat cattttagca gtaatgttat
    15601 tttctacggt tatatctttt acagcccctt ttttgcttac ttaagctgat tttaagtttt
    15661 ggcccttagt ctggatctac gaggtgagag tcaagaatga aaaaaataat tggagctttt
    15721 gtatttgcaa tcattatcat tattatctgt tttcaaatag atgtttttgg tcaaaaaaag
    15781 atagttggac aaacggtcag cagtacgaac agtacggtta caaagacaaa ggaatcattt
    15841 gatgatacag aacaagagac attaatcaaa atcggaaaaa atgaccgtac actatataca
    15901 gatgactcgc taactacccc acttgcaact ataaacggcg gagagctggc ggagtttctt
    15961 tctgaaacaa aaaatgctta tcagatcaag acaaatgatg gctatactgg atatttagcc
    16021 ctcgaagatg gaaccaaagt cacaaagaat atacaaacaa aaccggaaga gctatctgat
    16081 gcagtgattg tattagatcc tgggcatggc ggaaatgata ctggggcatt aagcaatgat
    16141 gaacaaacag aagaaaaaga tataacatta agtacagcca taaaagtaaa aaaggcgcta
    16201 gaggatgctg gagcaacggt ttatttgacc catactacag atgaattagt acagcttgga
    16261 gatatatgcg actacagtga agaaaaaaag gcagatgtct ttatcagttt acacgcagac
    16321 tctacggaat atgcgaatga agcaactgga atcacgacat attattacta tggacaagag
    16381 gaaacattag cacagacaat tgctgactcc ttcactgatc tgcctctgga ttcaagagga
    16441 atctccactg ggaactatca agttcttcga gaaaatcttc aaccttctat tttaatcgag
    16501 atgggttata tgaataatga tagtgattta aaggaattag taacagacag ttatcagcag
    16561 cagattgctg caagtttgac acaagcattg accacttatt ttcagtaata aataagccca
    16621 gccaaacaaa aatagctcct catatttttc gtttggccgg gcttattatc gcagatgcaa
    16681 gcttttaaac accgtttaag gaagaggtcg tgagcctgcc gttcaactct gagtcacaag
    16741 gagcaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag
    16801 ttggcatgag aacaccgttt aagaaaaagg ttgtgaaatc aacgttttgt tctgagtatt
    16861 agagaaacaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    16921 atcagatgat tgaacaccgt ttattcggaa caagggactg tgacatgact tttgtcacag
    16981 ttccattatt tttttagtag cttcaaagct ttttctgtat tggcaaaatg aagtttggca
    17041 tatttttcaa gcataggcat gccgcctttc tccaatagtt ttgccgctac ttggtcagat
    17101 ttcgttccag ctcctgacgg aagggtgatt ccagcttcag ctgatgcttt gtctaactct
    17161 tccaaaaagc tggcacgact gatgatagaa gcagcagcaa cagctaaatg atattgttct
    17221 cctttagtaa cgaagtacag tttttcttgt aattgatttt tttctaaacg cgcatatttt
    17281 ttaaaatttg cttccggcgt aaactgatcg atcaaaatcg cttctggttt ttctggctcg
    17341 atttttttta gcagcaaatg gatcgcttga ttgtgcaaag cgactttcat ccgaacggca
    17401 ttgtattttg gttggatctc attgtatttt tctggagata agacaagcag tttatagggg
    17461 atggtttttt ttaagatgtc agcaagctgg atgatttggc tatcttttaa ctcttttgaa
    17521 tctctaacac caagtttacg taattctgca agctgatcac gtttcacata agcagcacat
    17581 actgttagcg gaccgaaata gctgccattt ccgacctcat cactacctaa aacggataat
    17641 tgactgatat tttttggcaa agaagaggct gtttttgctg gcttggcttg tttatcctgt
    17701 gcctcccccc atttatcagc ttctattgct gcattcgtac cttgaaacat cacttttccg
    17761 gatgtataag cggtaatggt cgtcccgtct ttttttgcag caaagctagt ataaggaatc
    17821 tgtttattta acaaataaga gcgatagtat gctttcattt gactaagttg gtcgtttgtt
    17881 gctttgataa tgacgttgct cataaaattc tcctcgtttt tatacataaa actataccgc
    17941 aactcacgac ttttcgtttt ctaagagatt gcgagacata gcattttttt aaagtttatc
    18001 atataatagt taagtatgtg aaaaatattt gtggagaacg gtgaaagaac caacttcatg
    18061 gtacactaaa taaaactttc atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa
    18121 cacgctacaa agcggtgatt gccaaccaaa catatactat tatcggacgg gaaaccaagc
    18181 atcatatgga tatcgtaacg aagctgatca atgaacagtt agcagaattg aaacaattat
    18241 caccacaaat ggataatgaa caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc
    18301 aattgaagaa acaagaaaga atattggagc tggaagaaga aacagcagaa ttaaagaaaa
    18361 aaatgatcaa atttacagag cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg
    18421 aagctcggga agtattgaaa gaaaatggtc aagcagatca taccattaaa aatcatgtgg
    18481 aagcacaaca gattttaaac gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt
    18541 aagatccttt tatcaaaata agaaaaattt aaaaaagtta gaagagaaag gaacgtagca
    18601 tgctgagttt gctgatttta tttttattgc taattgcgtt tttttctgga gcacgtcgcg
    18661 ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt gtcatttatt gctgcccagc
    18721 atttttacaa accattagca agtcatttgg aattatacat tccataccca gctgtcaccc
    18781 caacatcaaa gttagcattt tttgatcagg tatttgcctt tcacctagat gaagcttttt
    18841 atgcaggaac tgcttttctg attattttgc taattggctg gctgcttacc cgatttgtgg
    18901 gggtttttgt ccatggattg acgtatgtgc cgatcttgag acaggtagac tggattgcag
    18961 gaggcatcct gtcactaatc atggcttacg ttactatatt tttgattttg cagttacttg
    19021 cctttgttcc attagatgtg gtccaaaatc aattcggcga gcatagtttc gctcgtttta
    19081 tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta acgaatattt
    19141 tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta gtttatttaa
    19201 gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga taaggtgata
    19261 aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag atggtcagac
    19321 aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca gtgagtgaga
    19381 aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc
    19441 gaggcgggat ccctatacct aaaattgaaa atatccgtcc ccatatgaaa cggattgaga
    19501 tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg tctacgacaa
    19561 gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga aatcgaattt gcacgtttgt
    19621 atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa
    19681 tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc ataagacaga
    19741 acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa
    19801 atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac gttatcccag
    19861 tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt gcttcaggtc
    19921 agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc
    19981 aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct gaacttgttc
    20041 cccatcgtca agaaatcatc cacaatactt atgtgattgg taaaatggat ctgatgaatg
    20101 caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc
    20161 atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta gtttctaatg
    20221 acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac acaggtggga
    20281 aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca ggattaccga
    20341 ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg gatataggag
    20401 acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg aacactgtag
    20461 atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa
    20521 cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt
    20581 ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga tataatcggg
    20641 caaatacgat taatgccagc atggagtttg atgtggatac attaagtcca acgtatcgtt
    20701 tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg
    20761 atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa gatttaaacg
    20821 aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat ctggaaatgc
    20881 gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct tacagctatt
    20941 tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaat gaagtcgtgt
    21001 cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga
    21061 ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc
    21121 tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa
    21181 cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg acgcttttac
    21241 gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag
    21301 acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc catgctgtac
    21361 gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa cgatacgaag
    21421 aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt tatcctcaag
    21481 tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact gattatctta
    21541 aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg
    21601 cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc gctatccaag
    21661 tatagaaatg ctatactagt catagatttt aaaaatggag gtctttgata tgtcacaagt
    21721 aattacagat aagacattta atgaagaaac agataaagga cttgtattga ttgatttttg
    21781 ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt tggaacaaga
    21841 atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc cagaaactcc
    21901 tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg gtcaagtagt
    21961 agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg atcaatattt
    22021 ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc gtcctcggtt
    22081 tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat tacgttgtca
    22141 ggctggattg tttttttgac agacatgcta taataacttt aattgaatat gaaggggagt
    22201 accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag cccggctgtt
    22261 acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa atattaaaaa
    22321 atcgtgtccg ctcatatttt actggtagcc ataatacgaa aacggaacgg cttgttagtg
    22381 aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg ctcttagaga
    22441 tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat gataaaacct
    22501 atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact cgtaaggtac
    22561 taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg aatgagacaa
    22621 agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag acgaaggagc
    22681 cttgtcttta ctatcatttg ggacaatgcc tgtgtcctta ttatttcgac gtagatccga
    22741 aagtctatac gaaaatcgtt gatgaagtga aaaacttttt aaacggagga cacgaaaaaa
    22801 tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag tttgaaaaag
    22861 ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc caaaaaatga
    22921 caaatactga tttactggat cgagatgtat ttggttatgc gattgataaa ggatggatgt
    22981 gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt tcaatgtttc
    23041 ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt tatcaggaaa
    23101 atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag cccagtgtag
    23161 aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa aaattagttg
    23221 aattggcgag taagaatgcc tcagttgctt tgaatgaacg gtttgatctg attgccagaa
    23281 aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc
    23341 caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct gtggctgcga
    23401 tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa
    23461 cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc agatattcgc
    23521 gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga ggaaaaggac
    23581 aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt cctattgctg
    23641 gcatggtaaa aaacgataaa cataaaacaa atgagttgct attcggtaca gatttggctg
    23701 tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc caagatgaag
    23761 tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt tttgcttccc
    23821 gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca
    23881 aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc ggactgccga
    23941 aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag ggcactttac
    24001 aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta cgttttgttg
    24061 gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct tgaagaagga
    24121 gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac acagctgtcg
    24181 atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc caaaacgggg
    24241 ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac caaggagaag
    24301 tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt tatcttccgg
    24361 aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat tttgcagaac
    24421 ttagagggaa atcaagaaaa gagattgaac caaaaatcga cttttggatg gaaaaatttc
    24481 aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat cagcaaaaag
    24541 tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat gagccattca
    24601 gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa ttgaaaaaac
    24661 atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa atctgcgatc
    24721 atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg
    24781 aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag gaagtagaag
    24841 aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag attaccttag
    24901 aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc tacattccta
    24961 tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca ggtgaaatcg
    25021 atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa gtcgatttct
    25081 ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt agcaggaaac
    25141 tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa tgagcaaatc
    25201 agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt tgattctgaa
    25261 gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt agtctcaact
    25321 gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca aagtacacaa
    25381 ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcgag tcaattaggc
    25441 atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg acaaaaagtc
    25501 aattttgatt caaaaggaaa aatgacagtc ggagaagata actcgacagt ccaatacgtc
    25561 gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc gcagatcatt
    25621 gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct gtccagtacg
    25681 actgcacaaa agcattttta cggaaaatta acaggtgttt tacttgttgc attaacgcaa
    25741 atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga tatggaaatg
    25801 gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt cttgtggtat
    25861 tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc tttatgtggc
    25921 tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac ttatctatct
    25981 ctaggcggat acatgcttgg attgatacta ggagcttcag atccaaacaa tatcgttatt
    26041 cggatcactt cgtatattcc attcttatct tcatatatta tgccgattcg cttagcaaat
    26101 gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc cgtaacattc
    26161 ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgttta tagcgaaggc
    26221 ggattatgga gttcattgaa acaatcgatt tcaattatga ggaatgaacg aaaaaaagca
    26281 taaaacaatc ctaaaataca gaaggaagct ggacatctat tgttcccagc ttctttttgt
    26341 atagacctat tttatttatt ttactatatt ggatatatat tcattgaaaa tagtgtataa
    26401 caactttata atattgagta taccaatttt tggaggcgtt ttaatggacc gttatttaga
    26461 tttactttca gaaaaattcc cctcaacgga ggatgtcatc acggaaatca tcaatctcga
    26521 agcaattttg cagcttccaa aaggaacaga gttatttttg agtgatatac atggcgaatt
    26581 tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga
    26641 ccttttttct gaacaaatga cggaaacgga tatcagccaa ttcactattt ttacggctta
    26701 tccagaatat gcattaacga ctgattggta caaagaacaa gataaatcgc agctgatcca
    26761 tcatttgatc gatcttttac ggtttacgac agtcaaatat acccggtcaa aagttcgaaa
    26821 aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg atgaccgtat
    26881 caatggaaaa aaagactatg taaaaaaaat cattgaacaa cttgttttga tgaaggaaga
    26941 agaaaaattt ttaacgaaac ttgcacagac gatccaaaaa tctgtcatcg atcatttgca
    27001 tatcgttgga gatatttttg accgaggaac tcaagcagcc aaagtgatgg atcgaatcat
    27061 ggatttttct tctgtagata tccaatgggg aaatcatgat attttgtgga ttggtgcgta
    27121 ttgcggctct gaggcatgtt tactgacact tttacggatt gcagcacgct ataattattt
    27181 atttgaatta gagaaagaat atgggttgaa tttacgtcca ttgttttctt ttgctcatca
    27241 aacttatcag aagaatccag tatttgcgcc taaaaacagt aaaaatctat ccgaaagaga
    27301 gcaagaagaa ttagaaaaaa tccatcaggc actttctatc cttcaattca aatcagaaca
    27361 tcagttattg gatcgtcgac ctgaatttaa aatggacgat cgtaaattgc tggaaaaaat
    27421 cgactatgaa gcatcaacga ttgatttaga aggtcagctc tatgagttga aaggaacttg
    27481 ttttcaaacg atccagccag atgatccaaa aaaattcttg gaagaggaac aaaaagtgat
    27541 tgatagtttg atgtattcat tccagcactc tttgagaatg caaaaacata tgacgttttt
    27601 gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtgctgtttc atggctgtat
    27661 acctgtagac gagaagggac agctcttagc atttgaatta gaccaatctt atcgcggaaa
    27721 agaactgctg ggcttttttg aaaaacagat aactgaaagc gcaaaagatc tgactgcaaa
    27781 agaagattta gcaaccgatt tgatttggta tgcgtggagt gggccatttt cgcctttatt
    27841 cggcaagagt aaaatggcga cttttgaacg ttattttctc acagagcctc atacgcacgt
    27901 cgagaaaagc aatccttatt atcacttgcg agatgaagag tggttcagtg aaaaaatttt
    27961 agcagaattc gacacaaaag aagaagggtc agcaatcatt aatgggcaca cgccagtaaa
    28021 agtaaaaaaa ggggagtcac cgatcaaagc aaatggaaaa gtatttgtca ttgacggagg
    28081 tttatccaaa gcttatcaaa aaacgacagg aattgccggt tattcactat tatgcaattc
    28141 ttatggtttc caaatcgtga cacattatcc gtttttgcct attgaacaac aatttgcaaa
    28201 aaaatccgac caaacaaatg tgaaaaaagt aatcgaacag caactaccac gcaaactcaa
    28261 tcgtgataca acaaaaggta tggaactgca gcagcagatt acccagctca aaaggctgtt
    28321 ggactatcga aaggacgatt gagaagaatt ccagtgaaca ttgttccata gactggagac
    28381 tgagacaaaa gtcgtgtcgc agtctctttt tccggataaa cggtgttctt ctgccaactc
    28441 ctcggtcaca agtcacacta ttcaacaaac atgaaaagct gttttttgaa attgctcctt
    28501 gtgactcaga gttgaacggc aggctcacga cctcttggta taaacggtgt tcaaaagcca
    28561 gtttctcggt attaagccaa atttatgcaa aaatgtgaaa agccattttc acaaaatttt
    28621 tcttaatact cgaaactaaa cagctttttc acaaccccct cagaaagtct attaaaattc
    28681 attcacaaag aaatcgaata agcgctgttc agatggatgt acacggtggg taagttccgg
    28741 atgccattga acaccgagga ttttatgcat atcatctttg ctttgaaccg cttcaacgag
    28801 tccatcgtta gaaaaagcga tggcttttaa taaaggtgaa agatctttga ctgcctgatg
    28861 atggtaagaa ttcacaaaat atggatcggg gagaaggttg cttaacaaac tgtctgaaac
    28921 aatcttgact tcgtgagtcg taaattgcgg ggcagtgggc tgttgttcat gttttactga
    28981 ccactttgga tataatgaaa gatcttgata aagtgtacct tccaaaacga cattcaatag
    29041 ttggagtccc cggcagactg caaaaattgg ttttctttgt ttcaatgctt ccaagatcag
    29101 tgcctgttca aattggtctc gttgaatatt tgtttccgtt agctttggat gaggatcttc
    29161 catgtaaagt tgtggactga catcttgacc acctgcaaga ataagtttgt cgatttgtcc
    29221 aatcagttga ggagctgaat ctggagacat gactggcaga ataagaggca atccttgagc
    29281 atctaaaact gcatccacga atccttgcgg tgtatagctc acttgatttc cttggaaagt
    29341 atctgttgct cgaatcagtt gatttccggc aatgccgatg attggtttca tttgtattcc
    29401 ccctaaagta gacttattgt aacaaggatt taaaaaaaat taaactatgt aagacttgcc
    29461 cctatactta tctgttcttg ctgtgttact atctgtaaag agaaatttcg tgtgaaattc
    29521 atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca gaaataaaaa
    29581 ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca ttttattgaa
    29641 ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa aaagaaagca
    29701 tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc
    29761 cgaaaagcag aagaagaaaa tgaaaagcgt gctcaaacac tgcggcagca gctgaagatc
    29821 tatgcaacag aaaaagagca ggaattggaa acgatccaaa caaactatcg acaacaacta
    29881 aaacagttgg tggaagaaaa agaacaattt gaaatacaag aattagaaaa agaaaaaatg
    29941 aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagatcaata tcaaaagcat
    30001 cagagtcaag cgatcggtgc gatcattgaa agggtgaaag aaacttatgg ccgtcactaa
    30061 gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt tacaagcagt
    30121 acaaggaatc caaaacgttg aaatccggga tctgtatcaa aatagaacaa acaaccagtg
    30181 ggtggagaag tattttccag ataccacgat tattgataaa gaagcaaaat taagcgcttt
    30241 aggtaatcgg ttgaaagaca tccaagaagc tatccaattc atacaacacc acggcgatag
    30301 ccgtcaaaag aaactccatt tgaaacgtca agaactttcc ttacaagatt tggaagccaa
    30361 ttattccgaa gaaaatttca taaataaatt gaaagaagtc cttgatttaa aagaaaagtg
    30421 ggaacaacta actgaagaac gtgatcgttt agaagaagag gaagaatggc ttcttcattg
    30481 gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt ttgagatggg
    30541 gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctgagtaagc ttgatgacgt
    30601 ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg ttgctttaaa
    30661 aaaatcagtt gctgttatag aagaaattgc tgggaaatac gggttcaata aagaaacgta
    30721 tccttatgaa caaaatccta aagaaaaact gcaagagacc aagcagcaat tatccattgt
    30781 tcacgagcag caaaagaaga taacgacagc gttaggtcag tgcagtggct atatccaaga
    30841 atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgagaaaaaa taaaagaacg
    30901 gtttatccat gcgacttatc tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca
    30961 gaatttgctg catgtgttaa acgaaaccgt atcagaagaa gatatatacg tcagtttcga
    31021 aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa accatccgct
    31081 tgtcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg aagaagtaga
    31141 tccaacacct tggatgacac cattttatct tgtcttcttc gggatgatgg tcgcagatgt
    31201 tggttatggt ttgctgatgc tcattgggac aatactagct caaaaactac ttgttctacc
    31261 acgcgggatg aaacgtttcg taaaattctt tgagattctc tcgataccat cgatcatctg
    31321 gggattgatc tacagttcat ttttcggaca gtcattacca aaagaactgt ttggcatccg
    31381 cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga ttttatccgt
    31441 gattttcggt ctgatacaga ttttggttgg attatttgtg gcagcaaagg aaaatatcag
    31501 gagaaaagat tacttgagtg ctgtcagtga tggatttgcc tggcaaggga tcctaattgg
    31561 gatcgttatt gcgttggttg gtgccatgtt gataaaaaat acagcatttg tttaccttgg
    31621 cgctgggcta tcgatcattg ctgccttatg tatcttgatc gttccgatta tccaaacgcc
    31681 gtctaaagtc aaaggtgctg ccaaaggtgc gtataatctt tacggactga ccggctatat
    31741 cggtgattta gtcagttata cacgtttgat ggcattgggt atttcaggcg gaagtattgg
    31801 agcggcattc aatatgctag ttgctttcat gccgccagct gctcgattca gtgtagggat
    31861 ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgctaa gtgcctatgt
    31921 ccatggcgca cgattgcagt atgtcgaatt ttttgggaag ttttatacag gaggcggacg
    31981 tgcctttgat ccgctgaaaa cggcggaaaa atacgtcaac atcaatcaca agaaaaaaaa
    32041 caataaataa ttggaggatt tttatataat gatggattac ttaattagtc aaaatggtgg
    32101 aatggtattt gcggtattag cgatggcaac agcgacgatt ttttcaggga tcggttcagc
    32161 taaaggtgtt ggaatgacag gtgaggcagc agcagcattg acaacaagtc aacccgaaaa
    32221 attcgggcag gctctgattc tgcaattgct tccaggcacg caaggtttgt atggattcgt
    32281 tattgccttt ttgattttta tcaacttagg aaatgacatg tcagttgtac aaggactgaa
    32341 ctttctaggt gcttctctgc cgattgcttt tacaggcttg ttttcaggta ttgcccaagg
    32401 aaaagtcgca gcggcaggta ttcaaatttt agcgaaaaaa cctgaacatg ctacaaaagg
    32461 aatcattttt gctgcgatgg ttgaaacata cgcgatttta ggtttcgtta tttccttcct
    32521 attagtgttg aatgcctaag cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc
    32581 atctctcaga tgaacgaagc agcagagcag gaaagagcgg cgttagaaca agaagagcga
    32641 atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaag ttgaaacaga acatcaaaaa
    32701 caaaaagaaa aacaaatcga attactcgaa aaaaaatacc gtcagctaag gaatcgccag
    32761 caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttaag acgattgttt
    32821 gcagatgcgg taactgaaat ggaaaattgg gatgaatctg aacaaatcca gtttatcaaa
    32881 aatgcattgt attcattacc tttgacagga aaagtagcat tcattgctgg agaaaaatca
    32941 gcagcctatc tttctcagac gttgttggat gaatggaaca atgaacttcc ctttatgatg
    33001 gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga tcaaggagtt
    33061 cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat gagttttgag
    33121 attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac gaactaaatc
    33181 ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa cggatgattc
    33241 aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac agtccatata
    33301 tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag agccgtctat
    33361 ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat acgatgcgtt
    33421 atactttcca taatctgaag gttttgacaa aagctgagat gacaaatcaa aatctggacc
    33481 atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc attcatacgc
    33541 aagtttcaaa tgaacttcct gaaagcatta tggacagtat acgagaagta cgtgattatt
    33601 ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag tttttgacag
    33661 agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa gtcattgctt
    33721 ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa agaacatatg
    33781 ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta tttttgaact
    33841 ttgtagataa agatatggaa gattacacga attttctatt agagacagat tatgctgatt
    33901 ttctgcgccc tgtcatccgt gagggaaata ttgatctcgt aaagcttgaa cagttgaaag
    33961 atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga ccactgccac
    34021 tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg
    34081 gcaaaagaag ccatttcgaa tcagaaacaa tcagaaagcg ggtgagacaa gtctatgacg
    34141 cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt gtttggcttt
    34201 gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga aatggcaaag
    34261 aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc agaaacgatc
    34321 gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca tcaaggatca
    34381 ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc
    34441 ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa agtctccgga
    34501 cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg tctagtggga
    34561 gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc tattcaagta
    34621 tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg tgaagcttta
    34681 tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca acggccatta
    34741 gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca attgcccgca
    34801 ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga aacggtgagt
    34861 gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa aatcatggtg
    34921 ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac tattgatgaa
    34981 acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg
    35041 ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc catgatcact
    35101 ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc agctgtacct
    35161 ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg
    35221 gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga tgttttaaat
    35281 gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg tacggtattg
    35341 attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta tacgggaatc
    35401 acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc agactccact
    35461 tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat gcctggtgat
    35521 gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg atctggaaga
    35581 gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc
    35641 ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt cgtaaaagta
    35701 ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat cgattggata
    35761 caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat ccttcaagct
    35821 gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca gcagctgaat
    35881 gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct gacattagaa
    35941 gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga tgtcgataca
    36001 ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt tggtgaagaa
    36061 ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg taccgtatcg
    36121 attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga aaaactagat
    36181 caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg aggcatgagc
    36241 aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa
    36301 agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg gcgagattcg
    36361 tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat tcgaaggaac
    36421 aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt tagaactagg
    36481 tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga aagataacgg
    36541 accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca atccagtagc
    36601 tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc atttgaatac
    36661 attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc ataaagaatt
    36721 agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt tcgccgtagt
    36781 ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag attttcgtca
    36841 aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga
    36901 acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt atgaaaaggg
    36961 aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat tgcgagagat
    37021 ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc tttacacgaa
    37081 cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat cggttactca
    37141 aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg acttgactgg
    37201 ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg ggattcagcc
    37261 accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg gacccggaaa
    37321 gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa
    37381 acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg tagacaagat
    37441 ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa
    37501 ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt tgccaagaac
    37561 cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag ggaagtgagt
    37621 tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca
    37681 gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg aactgatgcg
    37741 tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg aagagtctat
    37801 gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac ctttcatcga
    37861 agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca aaaatatcat
    37921 gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag acacaccttt
    37981 ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc ggtttactac
    38041 cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac tgatggcgag
    38101 tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga tccctcaatt
    38161 agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac
    38221 aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt tttttaaaag
    38281 aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc tgttcagctt
    38341 ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac cctaccattt
    38401 tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc aacttctgct
    38461 gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag tgctggtcaa
    38521 tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt tccgattcta
    38581 tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt aaaagaagcg
    38641 ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct aaaattggcc
    38701 tttgtgatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc tgaatttggc
    38761 tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg taatgcaggg
    38821 tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt gatcatggtg
    38881 gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga tttactggaa
    38941 tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt tacaggtacc
    39001 cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc aacgctagtg
    39061 gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac acctagaacg
    39121 gctggatatt attcagttga ttacctccat atgagccatg caggtctgat tttaacgatg
    39181 tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa aacaactacg
    39241 ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag agcggaagca
    39301 tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt ttttgttacg
    39361 ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat acctaaaact
    39421 tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg
    39481 atgggattga cgccagactt gactgcaata gggaaattga tcattatagc gcttatgtac
    39541 attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc acaaagaaca
    39601 caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca tcttctgaca
    39661 gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt cttggatact
    39721 ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac ctcgggtagt
    39781 gcctcttttt tattgaaagg aatcaataag atttgattcc tttatttatc aataggttat
    39841 aattcatatt tttttatttt ttcttttacg atacttagtc cagaagctaa aatttcaagg
    39901 ttattagagg atagggaaat tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt
    39961 ctggaatgat aaagtcggat accgttagca agaaagtcat tctcaattat cttagggtca
    40021 atctcaggat caactggaag aattcgatag taagggacac gattcgttgg tttttctact
    40081 ttgaaaagag aatcaaaaag ctgattggct ctttttgtaa gacttaattt agtttctaag
    40141 atcctatttg cttcatttga atggaggatt tgagtgatga cttccacatc taatccagaa
    40201 gttttcacat ttatattaaa aattgcttgt tctatcttgc tttggacatg tactggaact
    40261 acaagataag caactcgcaa tccagaacaa ataaattttg tcattccagc taagtagatt
    40321 gtttggttag gtaataaatt ttggaaagga ggaatgatcg tatgttgata gtaagttgtt
    40381 aaaaatgaat ggatatcatc ttcaataacc catagacctt ctttttcaat cacttcggct
    40441 aattgctccc gtcttttgtg agagatttga aaccctatag gattattaca agaaggcata
    40501 agaaagacac catgaatctt cttttttcta cattcctgaa tcaatatatc aggtcgcatt
    40561 ccttgctcgt caaaatcaat tggaacgatc tctaaatgat ataactgagc caattcaatg
    40621 aaattagcgt atgtatatcg gtctgttgct attctatcac ctggagagaa aagtgcagaa
    40681 agtgtgatag ccaatccatt ttgaacaccg gaaacaatag atagagattc taaggacgga
    40741 atgactcctt gagtttttaa ccaatttgct gcagtagtca attgaaaatt tgtgcctttg
    40801 ggatcttggt aattaagcaa accaacaaga tttgggttat tactagttga ttgaataaac
    40861 gggacaacga atttattaca ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt
    40921 tcggttactt cattggtaga gatggtgatg gactcaaaga cattgggaga aataaatgtg
    40981 ccacttccaa tattcgtata cagcaaacct ttctcaattc catatttata agccttgcca
    41041 acggtagtaa aatttaaatc taaataatca gctagctctc tttgggaggg taactgagtg
    41101 tttttctgta atttattctt taaaatatct tgttctaatt gttgaatcaa cgatcgataa
    41161 taaggacgct tcaatttttg tttgtctggt cgccaagtta acgggtaatt gtcataagaa
    41221 ttaataggca ttttctccac tccttaaaat tgtataccat acaattttac cattgattgt
    41281 attgatttta gaagttaatc ttttgtgagg agggaattgt ctatgaaaat tattaatttt
    41341 gaggaagtag tagcttcttt tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt
    41401 tttgattatg aaagtggtaa aatcagccaa aatcctcgat tggtggaaat tttacctgac
    41461 ggaaaaggca aaaatatgtt tgcgatgatg cctgcttatt tgggggagaa cagatatttt
    41521 ggcacaaaag tcattaccgc ttttccggat aatcatcagt atcatttacc ttctcattta
    41581 ggagagattt tcttgtttga tgctcaaaac ggactaccca aagcattgat aaacgcaaat
    41641 gcagtcacct ggattcgaac agctgctgta tctgcgctgg caacagatta cttagcaaat
    41701 ccgcatgcag aaacacttgc tttaattgga gctggtcaac aagcattgtc gcatttgact
    41761 gcgatttcta ccattcgtcc catcactaca gtgtttgttt acgatatttt agaagaaaga
    41821 cgaaatactt ttatcgctaa agctaaaaaa cagtatccta aaataacgtt tataaatgca
    41881 atatcagtag aagatgcagt acgtccagcg gaaatcattt gtacgttgac ctctagtaaa
    41941 gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca
    42001 tttactcctg atacaagaga gataagtaca tcgttgatgg aaacttctca aatctatgta
    42061 gatgattact cagctgcctt aacagaaagc ggggacctgc ttatagccat ggcagaagga
    42121 gatttagagg aatcttgtat tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca
    42181 ttttataaag gagaatcgag gccgacgatt ttcgacgcag ttggtctagc tattgaagat
    42241 ctttgttgtg cagaatatat ttataacaaa ttagagaaag gggctttaaa atgaacttag
    42301 attattacgt agttgatgca tttgcagatg aggtgttcaa aggaaatcca gcagcggttt
    42361 atgtgctaga agaatggctg ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt
    42421 tatctgaaac agcatttaca gtgaagaaaa accaagaatt tgagcttaga tggtttacgc
    42481 cagatcgaga aatcgatctt tgtggtcacg cgacattagc aacagcattt gttttattta
    42541 actattacaa aatacctgat gaaacgatta aattttctac tcaaagcggt aatctatttg
    42601 tgacaagaat taaagattat tattacatgg attttcctag tattatgccg aaaaaagtgc
    42661 cgatattgtc tgaatatgaa gaagcgattg gggctaagat taaagaagcc tacttagcac
    42721 gtgatttatt tttcgtatta gaagatgaaa aaacggtggc taaattgcag ccagatttta
    42781 cagctatcaa aaattttgaa ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa
    42841 aagactttgt ttctagaacc ttctttccaa aattaacaat caatgaagat ccagtatgtg
    42901 gatcagcaca ttctaatctt attccttatt gggcagaaag aatgaataaa acaaaattgt
    42961 cagcttatca aatgtcatca aggggcgggg atctagaatg tgaattaaaa gaagatcgtg
    43021 tgataatagg aggaaaagca gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt
    43081 caaatgaatt aaaaataaat tggtggagag tggcaacctt atagctacaa tggttgttaa
    43141 aaaaagacgc aaactttttc aatttaatga aaagtttgcg tctcatttta attattggtg
    43201 tttgtttttt attcaatcaa gagtaaaatg ccccatacta actatcctcg gatgacttcg
    43261 attttataac catcagggtc aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct
    43321 tttaagtcag taatctcaag accagcagct ttatgttgct cgtgtaattt ttctagatca
    43381 tcagctgcta tagcgatatg accatagccg tctcctagat catacgcacc atgatcatag
    43441 ttgtaagtca actctaattc atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat
    43501 ttgttttctg gaaaatcacg gcggcggctt tcttcaaaac caaatgcctt ttgataaaat
    43561 tcgattgatg cttcaagatc tttcactcga acacatgtgt gagccatttt catagattca
    43621 tctctccttt tccttttact caatcataac aaaaagaagt aaggaaatag ttgatttgtg
    43681 cttgttcatg agaaatttct catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa
    43741 ctattcatgg ataaaccaat ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt
    43801 tatgcagcat atattgtcat agaaagagat cagcagattg cagtaatcga ggctccaaat
    43861 ggtgcttttt ttcttcctgg cggggaaatt gaaggagaag agacgaaaga agaagcgatt
    43921 gtccgtgagc tgatagaaga aatgggaatt gtggcagaaa tttcttctta tctgggagaa
    43981 gctgatgagt atttttactc gaattatcgt gcaacttatt attataatcc aggatatttc
    44041 tttgttgctg attcttggaa gcgtatagga gaaccaactg aaaagaccaa taaaatttgg
    44101 tgggtgagcc ctcaagaagc gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc
    44161 gagagatgga tcgaaaaaaa taaaaactga aagaaagcga gtagatcagc tttctctcag
    44221 tttttttatt tattacagta catatttttt gattgcttca gctacaccgt gctcgtcgtt
    44281 tgatgaagtg atgacatttg ctgcttcttt gacaagagaa acggcatttc ccattgctac
    44341 tcctagacca gcatattcaa tcatggataa gtcgttctcg ttatctccga tagccatgat
    44401 ttcatcttga tcaataccaa ggtgctgtgc gagattcgca actgccgcac ctttacttgc
    44461 ttctttattt agtacttcga agtaataggg cgcacttttg accgttgtgt atttatcacg
    44521 gaaagtttgc ggtaaacgcg caattgctgc atctaaaatt tctggttcgt cgatcatcat
    44581 cattttgacg atgttcatgt ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat
    44641 cttgactaaa cttgcttcgt ggatcgtata agcgctgata tctcgattag gcgtgtagat
    44701 cgctttgtca tcgatcgaat ggagatgact gccgacacgg cgtgccatta cttcgatctc
    44761 taggaaatct tcatgtgtca gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg
    44821 tacgagtgcc ccgttataag taataacgta atcattatct tgaaaaaggt tcaactcgtt
    44881 taactgttcc tggacacctg gtaagggacg gccagtgcat aaaacgatcg aaacaccttt
    44941 tgcaacagca gcggcaatcg cttcttttac ttcttttgtt acttctcttt tctcatttaa
    45001 taaagtacca tcgatgtcga tagcgactag tttgatagac ataatttcct ccaatatatt
    45061 ttaatttatt tcaatcaatg cgccattacg gatatggctg gcaaattctt gataagtttc
    45121 gtcaaataaa tcataatgat cacgcaagga cggatcaacc atttcttttg gaaagaaaaa
    45181 tcgctcatct ccttgttctt gtcccgctaa tgcagcaaca agctgactaa cctgtgataa
    45241 ttctatcaaa gttccgtcat ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc
    45301 tgggcgataa aaatcataag gtagatcgta gctagaattc acagcagtgt aataaacagg
    45361 attatatccg accttttcta ctaacaaagt gagttcttgg actaattttg aatcccggtt
    45421 gccagaaaaa gtagcagatt tcagtggttt acgattcaaa aaacgtttag ctaaatcatt
    45481 taaaatagga tcagcagatt cagaccattg agtaaagtaa gtgcccaaga ccccatcatc
    45541 aagtttgagg tattcttcta aagtaaagct tccgtcaaga aatggcagaa gtaattgaga
    45601 atggagttca aaactgctgg catcttcttg atacaactcg tgtgcgcgat gaagcagatg
    45661 ctccaaaatt acttccatac cacgggaaac aggatggaag tacacttgca catacatttg
    45721 atatcggcta acgatataat cttccactgc atgcatgcca ctcatggaaa aagcgatccc
    45781 gccttcataa ggacgaatca cacgtaatat tcgggtaaga tcaaatgttc catattctgt
    45841 tccagtgaaa taagcatccc gaagcaaata gtccattcga tctgcatcga tctgactaga
    45901 aatcatttgg acaacttgtg ggttggggta agttttttgg atcacactag ctaccttttc
    45961 aggaaaacct tcttctactc ggttaagaat ctgatacact tctgtttctg gagaagtgat
    46021 gatctctaca gtgatggctt catgattcgt atggaagata tgttcaaatg tatgagagta
    46081 aggaccatga ccgacatcat gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc
    46141 atcccagcct ccgttgccta ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat
    46201 tcgtcttgaa atctcataaa cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg
    46261 aaaagtgaaa gaagaagtgc ctaattgttt aatgcgtcgt agacgttgga cttctttcga
    46321 attgatcaaa tctaagatta cttgatgctg gacatggata tagttatgga ctggatcacg
    46381 aaaaactttt tccataggca gcatttgata tttataaggg gttgtcataa tgtagctcct
    46441 ttatgttatt acgttgttcc attctctgga tttggttgtt ccattgctcc agctggattt
    46501 ctttttttag ccagtcggta gaatcctgta aatcacttgc agaatacagc ttttcaaaag
    46561 cttttgcaaa tgcttctttg acttctttta caaaaagggg ttggtcaagc agttcttcaa
    46621 gtgttgccat tgtagcagga tccactgcag gatagccatt tgtaccaaag tcttctttta
    46681 aagcagattg ataaaatcgc ttcatgactt gcccacgttt ttcctgatta ccgttgacac
    46741 ttaagtacat cataacagaa atcccatttt tgatccgacg ctgagcgata ccggcaaatt
    46801 ttttgccatg gatgcttaaa tcataagttc caggacaata agaatgggtg atttcgaatg
    46861 cttgtatcgg tgcctttgga aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt
    46921 catctatggt tatttttttt gtgtcacttt gggggaaaat caaagaaata ttcaaaattc
    46981 cttcatctga tacgatccct aatccacctg aatttctgat cactggagcg taacctgcat
    47041 cttccagtgt ttggatcccg tcacttagaa aaggagttcg aacatccttc atacctaaga
    47101 tgactgtgca gtcatattgc cagaaatgta aaaagatttg ctggtgttct ttagaatatt
    47161 ccgttaatat atctgttaat gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat
    47221 ctagtcgaat cgcagggttc attcaaccgg ctcctttgta taaattcata ttttctacaa
    47281 tattatactc taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa
    47341 gttggttgac aaaacaaaag aaactaggaa caatgtagct atgagaagta aaggagaatg
    47401 aataatggaa ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca
    47461 gcaaggggaa gtgcaagatt tttattttga tttgaaaggg caaatggtga caattggcga
    47521 tacgctgtac atacgttata aagagattca ggaggagacg ggcgatgaga ttcctgtaac
    47581 gatcaaatta gttccagatg gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg
    47641 tttgaaattt ggctataaag aacggttaga aacatcctat cgaacaccat acggtatgat
    47701 ccagattgct acttttacga aagaactgca tgttagtttg aaagaccgtc ccacagcagg
    47761 gaaggttcga atcgattatg atctgttcat gggaccggaa aaaatcggcg aatattattt
    47821 gaccttggat tttacagcgt aaatataaaa gaaaagtttg attttttgtg tctgattttg
    47881 tatgataagg agtagactgt gaaaggacgt gtcctgattg gaaattagtg tatttgaagg
    47941 tgcaaacaaa agcgaattat caatgatcga agtagcacac gcgatcttag aacaacgtgg
    48001 cgatgtcatg gatttttctg atttagctaa ccagatccaa aactatcttg ggaaatcaga
    48061 cagtgaaatt cgtgattcac tcgcacaatt ttatactgac ttgaatattg acggcagctt
    48121 tatctcatta ggggataatc gttggggctt gcgttcatgg tatgctatcg attcgatcga
    48181 tgaagaagta aaccatggaa tggacgaaga ggatgaagat actccacgtc gccgtaaacg
    48241 caagaaagtc aacgccttta tcaatgatga tgaagacgcg atcgattaca atgacgatga
    48301 cccagaagat acagatctga cagaagaaga tgacgatgat ctgtttgatg acgatgatga
    48361 tgaagatgaa gaaatcgctg cttataattc tgacttgcaa gaaatcggag cagatgaccc
    48421 gacagatgat gaagaagact tgccaggaaa tatcgaagaa gatttaagta tcatagacga
    48481 agacgatgat gatgaagatt atgaagagga agaattttct gactctgaaa aagaataaac
    48541 attattttct tacgagaggt tgtgacagag acgatcagcc tatcagaata aggaggtgtg
    48601 acacgatttt tgtcacacct tcttttttgt atataaaaat cccaagtaaa tacataaaaa
    48661 ataaaaaaat tgaaactttt agcgcacctt gtacgtcata ttaagtgagg cagaaaggag
    48721 tggagaatga tttttactgg atataagaaa aaacggcaga tcgaaaaata ccgtcacctg
    48781 ctgaatggac tcatcgaaac tgattatcaa aaaatgttcc gtatcacgat gaattacgta
    48841 catgataaag aagaggcctt agatgtgatg caagatagtt ttcataaagc cctgaactat
    48901 tttgaaaaag aaaaagagat cgagcatttt agtgcatggt tttatcggat attgatccgc
    48961 acggcattag atagttggcg cagacaaaaa aggacgcctg tggacttatt tgaaattcaa
    49021 gatatccccc aacagttacc tttagaaact tccgttgcag agctccatat gattttagat
    49081 aaaatagaca gtccggaaaa agagattctg atcctctact tttttgaagg atttcgtttg
    49141 aaagaaattg cgactatctt ggatatgaat gaaaacacag ttaaaacgaa aatgtaccgc
    49201 tcattgaatg ctttacgtca aatcctagaa tgaaggagag aagagtgcca tgtattccca
    49261 aaaagaaaat ttaaaaaaac taaagaaaac ttataaagaa caagcaattc ccaaagggct
    49321 taaagaggag atccgccagc gttttattat cgaagagcaa tcatttataa aagcaaagcg
    49381 gagaaagaga cgtctccaag ggttcggagt atctttggct atcgtaatga ttacaacaag
    49441 tagccttctt tttaatagtc aagttcgaag ctttgcagaa gatttaccca tcttaggatc
    49501 agtcattgaa ttgatcttag gcgaacgctt tactgatcga tctgaaaaaa tcgatattca
    49561 ggtaccgaag atcaatacac aaaatgagaa agaaaataaa acaattcatg gattaaatca
    49621 aaaatacttt cgcgaaggac aagcagattt tgaaaaagct gaaaaagaat atggaaattt
    49681 tgaaacggat cattaccaag ttttgggaga ttatcagaaa atcgtagacg ataaccggtt
    49741 tctcgtgatt gaaaggcaga tcacccaaac agcagcagat agccatgttg aaaaaagata
    49801 cgacacaatc gacaagaaaa acagtgtcca gctctcactt ccacttttat tcaaagatga
    49861 cacctatcta tctgttttga caaaagaagt caaacggcag atggccgaac aagtcaagga
    49921 agatccatct aagtactatt ggactgagca agatattcaa gaagggacga tagaaaaacc
    49981 aacacttgtt acgcctacac gaagttttta tttgaacaaa gagcatcaat tagttttgac
    50041 tttttctcaa tacgaaattg ctcctggtta tatgggtaca ccagaatttg tcattcctaa
    50101 gtccgtgaca aaaacgattt tggcatcgga agattatttg gatcattaga cgagaagtag
    50161 ttgcgactag cagtttcaat tttcaagtaa gtacaagtaa ataataagaa gagaaaaaga
    50221 gaaagaagag atatagtgaa aagaaattat ttagttatca gtattattgc tttatccgtg
    50281 cttttgtttt tcgaaattac ttcggtggcc tccttgatca ccgaagtaaa aggacaaaca
    50341 cactcttcgg agcattcgtc acaatctttc aaacaatcag aacaatcaac cattaaaagc
    50401 caatcaactg aaacagctaa cgtcaaagaa gccattcaag aatcagcgga aaaggtacaa
    50461 agtcagtcat cacaatcaac acaagacagt acaaaaacga gccgagatcc ggctgtcaaa
    50521 agcgtcgcaa tcagctttga tgatggacca ggagcgacca caacaccgca gcttctgcga
    50581 atcttaaaag aaaaaaacgt gcatgccact tttttcgtct taggagaaaa tacggcgcag
    50641 catcctgaaa tcgtgaagca gacagctgaa gcagggcacg agattggcaa tcacacatac
    50701 gatcatcaga atctggccac tctttctgcc cagtcgatga cggaggaagt gacgaaagca
    50761 gacactgaga tcaaaaaagt cactggcaaa acccccactt ttgttcgtcc gccctatggt
    50821 tctgtcacga atgtaggagc aacgatcatc cagcgaccga tcatcgaatg gtctgttgat
    50881 tcagaagatt ggaagacaag aaatcctgat ttgattttgc agaagatcca agcaacagta
    50941 tacgatgggg caattatttt attccatgat atctatcctg aaacgattcg tgccgttcct
    51001 caagtgatcg attatctgaa agagcaaggg tatcgcatca cgacagttgg ggatttatta
    51061 gggcatccaa cggctgtcga gaactattat ggcagaaacg atcatcggcc agttcagtaa
    51121 gtgagacaag tatctttaat aaataacaca ttttaataag tagttgaaca cataactacc
    51181 ttacttgcaa aggcttaaca atatctatac aggtacaaca aaaaactgtc caccaatttt
    51241 ctttggcgga cagttttcac ttaaaaataa agaagcaact attcgtaaat tatgctaaaa
    51301 tagcagtaga acgcgtcctg agggaatcga acccccgtct caagaaccgg aatcttacgt
    51361 gatatccact acactaagga cgcaagcact gggataattg taactaaaca gataaaaaat
    51421 tacaagggga ttggacaaaa aaatgaaatt tgagcaaaat ttttcacaaa aacagcagca
    51481 gacacagaaa ctagccatga cgcaacagtt gcaacagtcc atccaagttt tacaatataa
    51541 cagtgaggag ctgttggcat tcgttgagaa tcaagcaatg gaaaatccat tggtggaagt
    51601 tgttgagccg gaatggcagc cagattacat aaaagcttct tcatcttctt atgaaggaga
    51661 agagacaaat tacctaaatc agattcctga tacaaaaggg tctttatttg attcattgat
    51721 cgaacaagtc catttgaatt accgagatac ttttttgagg aaaatcgttc tttatcttgt
    51781 agaatacatc gatttgaacg gatttttgac gattagttta gaagaagccg gaaaagagat
    51841 tggagctacg cctatccaaa tgctcgatgc gttgactctg attcagcaat tagaaccagc
    51901 tggagtaggt gcacgtagtt tgcaagaatg tctcatgctt caaacagaac gagatgatta
    51961 tgcgccggaa ttagcttata tcgtgttgga agaatgtttt gaggaactgg tggagcgaaa
    52021 atggaaagaa atcgcacaac gtttttctgt tgatctacat gctgttcagc agatattcga
    52081 ctatttacaa acgttgagtc caagccctgg ccggattttc gaccgtagca gcgaattgta
    52141 cattattcct gatgtccggg tattagtgga cgacgataaa aatgtacaag ttatctcaaa
    52201 ccgaaaaaat cagccaaata tacgctttca agagagttac ttcaaacaaa tgtcacaaca
    52261 agcagataaa gaaactgaaa attatttaaa agaacggaaa caggaattcg aatggttgaa
    52321 gaaaacgatc ctacaacgtg gggatacaat tttgcgggtt gctcaagtca ttgtttcccg
    52381 gcaaaaagct ttctttatcg acaaggaacg tccaatcaag cctcttacgt taaaggaagt
    52441 tgcaactgag atagatgtgc atgaatccac ggtcagtcgt gcagtcaacg ggaaatattt
    52501 ggaaacagac ttcggtgtat ttgaattaaa aaaatttttc actacacgaa ttcctacgaa
    52561 tagtacagaa cagacagaag acctttcagc agatacagcg aagaaaaagc tgcaggaatt
    52621 agtcgatcaa gaagacaaaa acaagccgtt gtctgaccag aaattggttg aattgctgaa
    52681 aaaagatgag atagcgatct caagacgaac agtagcaaaa tatcgtgatt tattaggaat
    52741 cccgagttca tcaaaaagaa aacgttatga taactaatca gtcaaaagac tagaaaaagt
    52801 atttcacttt ttctagtctt tttagttgaa aaacgagagc aaaactgtta gacttacagt
    52861 cgtgaggttg agatttccct ttattgatgt gaaattcgaa tagttaattt ctttttttat
    52921 cgtatatggg tcgttttaag tctacattgg acgtaaagtg tccaactaag gagatcgcta
    52981 tgctggatga aataaaaatg attgaatctg ttgcaccaga tattatcgaa gtgttgcaag
    53041 aacgctataa aattttacga aatatctatt ggatgcagcc gattggacgt cgaagtttat
    53101 cggaaagtat ggggatcaca gaacgagtat tgcgaacaga aacagatctt ttgaaaaatc
    53161 tgaatttgat cgacacctca aaaagcggta tgacgctgac aactaagggc gaagaagttt
    53221 atcaaagcct tgaaaacttc atggatcagc ttttaggtac gcatcaaacc gaacaacagt
    53281 tggcagaata ttttggtaac caacgctgta tcgttgtttc aggaaacagc gaagagcaga
    53341 ccaaagtggc tgatgcattc ggcgaagcgc tgagcgaagc cctagaccga ctacttccag
    53401 aaggcgagaa tatcatcgct gtgatgggag gaactactat ggctgtggta gccgaacaat
    53461 tgtctaatct ggaaaataag aaacggcata acctgtttgt ccctgcaaga ggcgggattg
    53521 gtgaagcgat aactgtacag gcaaattctg tcagtgcaag aatggctgct aaagcaaacg
    53581 gtaaccaccg tgcactttat gttccggaac aattaagtct ggcaacttac aattctttac
    53641 tcaatgagcc atctatccaa gaagtcttaa acttgattag tgaagcgaat tgtgttattc
    53701 atagtattgg gcgtgcatta cacatggcag cacgtcgtaa gatgactgaa aaagaattag
    53761 tcatgctgaa acaagcaaat gctgttgcgg aatcttttgg ctactttttc aatgaaaaag
    53821 gtgaagtcgt ttataaggtc cctagaatcg gcatagaatt gcaagatttg gaaaaagtcc
    53881 cgatcatcat ggcaatcgcg ggcggaaaat caaaagccaa agcaattcga gcttatatga
    53941 aaaatgcacc aaaacaaacg tggctcatca ctgatgaagc tgctgcaaat gagattttaa
    54001 aaggggtaac cctttaaaat aaatattttt ttgattttca taaggaggaa atcttaaatg
    54061 acagttaaag taggtattaa tggttttgga cgtattggac gtctagcatt ccgtcgtatc
    54121 caagatgtag atggaatcga agtagtagca atcaacgact tgacagatgc taaaatgttg
    54181 gcacacttgt taaaatacga cacaactcaa ggacgtttca acggaacagt tgaagttcat
    54241 gaaggttcat tcaacgtaaa cggaaaagaa gttaaagtat tagctaaccg caacccagaa
    54301 gaattaccat ggggcgaact aggcgtagat atcgttctag aatgtactgg tttcttcact
    54361 tctaaatcag cagctgaaaa acatttaaca gctggtgcta aacgcgttgt tatctctgct
    54421 cctggcggaa acgatgttcc aacaatcgtt tataacacaa accacgaaac attaactggt
    54481 aaagaaacag ttatctcagg tgcttcatgt actactaact gtttagctcc tatggctaaa
    54541 acattgaacg acaaatttgg tgttgttgaa ggattaatga caactatcca cgcttacaca
    54601 ggtgaccaaa tgactctaga cggacctcat cctaaaggtg acttccgccg tgcacgcgct
    54661 gctgctgcaa acatcgttcc taactcaaca ggtgctgcta aagctatcgg tttagtaatt
    54721 cctgaattga acggtaaatt agacggagct gctcaacgtg ttcctgtacc aacaggttca
    54781 ttaacagaat tagtaacagt tcttgaaaaa gaagtaactg ttgacgaaat caatgcagca
    54841 atgaaagaag cttcaaacga atcttacgga tacaacacag acgaaatcgt ttcttcagat
    54901 atcgttggta tgacttacgg ttcattattt gatgctacac aaactaaagt aatgacagtt
    54961 ggcgacaaac aattagttaa aactgttgct tggtacgaca acgaaatgtc atacactgca
    55021 caattagtac gtactttaga atacttcgct aacttataag atagacaaac gtacaagtgt
    55081 aaattataga catgtacatt taaaagcggg gaagcaatcg cgcttcttcc gctttttttg
    55141 ataataagtt tattcaggag gaaataaaat ggctaagaaa acagtgaaag atatcgattt
    55201 aaaagacaaa aaagttcttg tccgtgttga cttcaacgtt cctttgaaag acggcgtgat
    55261 cactgacgac actcgtatca aagcagcatt gccaacaatc aactacgttc tagaacaagg
    55321 cggaaaagca atccttttct ctcaccttgg acgtgtaaaa acagaagaag ataaagaagg
    55381 aaaatcttta gctccagttg caaaacgtct aggcgaatta ttaggtaaag aagtaacatt
    55441 cgtacctgaa actcgcggag aacaattaga agaagcaatc cgcaacatga atgacggcga
    55501 cgttgtcgta ttcgaaaata cacgatttga agatattgac ggtaaaaaag aaagcaaaaa
    55561 tgatacagaa ctaggtaaat actgggcttc attaggcgat gtatttgtta acgatgcttt
    55621 cggtacagct caccgtgcac atgcttctaa cgtaggtatc gcttcaactg gtatcccaac
    55681 agttgctgga ttcttgatgg aaaaagaaat caagttcatc ggtgaagcag tagaagaacc
    55741 aaaacgacca atgattgcaa tccttggtgg cgcaaaagtt tctgataaaa tcggcgtaat
    55801 cgaaaacttg attcctaaag cagataaaat cttgatcggt ggcggaatga cttacacatt
    55861 ctacgaagca aaaggaatca agatcggtaa ctctctagta gaagctgata aagtggaact
    55921 agcaaaagaa ttaatcgaaa aagcaggcga caaacttgtc ttgccaatcg acaatgtctg
    55981 tgcaccagaa ttttcaaatg atgttgaaac acaagtaatc gaaggagaca ttcctgatgg
    56041 tttgatggct ttagacatcg gcccagcatc agtaaaatta tttgctgata cattacaagg
    56101 tgcaaaaaca gttgtttgga acggacctat gggtgtattt gaaatgagta actttgctaa
    56161 aggtacaatc ggtgtttgtg aagcaatcgc taacttagaa ggcgcaacaa caatcatcgg
    56221 tggcggagac tcagccgcag cagcagaaca attaggattt gctgacaaat tcacacacat
    56281 ctcaacaggt ggcggcgcaa gcttggaatt gcttgaaggt aaagaattac caggactagc
    56341 agcaatcaac gataaataaa cgaccaagtt tgctcaattc tgacgagtaa tcgatcaaaa
    56401 ggtgaaggga gttttcatga ctccctagcc atttttcaat aaattaagga gtgtgttcct
    56461 caatgcgtaa acctattatc gcaggtaact ggaaaatgaa caaaactgca tcagaagcaa
    56521 aagaatttgc tgaagcagta aaaaacaaaa tcccttcaaa tgacaaagtt gattcagtca
    56581 tcggatctcc tgcattattc ttacaagaat taatgacatc agctgaaggt acagaactaa
    56641 aaatctcagc acaaaactgc tactgggaaa actcaggtgc gttcactggt gaaacttcac
    56701 cagcagctct tgctgatcta ggtgttcatt atgtgatcat cggacattca gaacgtcgtg
    56761 aatatttcca tgaaacagac gaagacatca ataaaaaagc gaaagctatc tttgcaaatg
    56821 gtatgacacc aatcctatgc tgtggcgaat ctttagaaac ctatgaagca ggcaaaactg
    56881 ctgaatggat cgaaggacaa attacagctg gtttagctga tctttcagct gaacaagtgt
    56941 caaacatggt tattgcttat gaaccaatct gggcaatcgg tactggtaaa tcagctgacg
    57001 cacaaatcgc tgacgaaatt tgtggagttg tacgtcaaac agttgaaaaa ctttatggaa
    57061 aagaagtatc agaagcagtt cgtatccaat acggcggttc tgtaaaacca gaaaatattg
    57121 ctgaatacat ggcaaaagaa aatgttgacg gtgctttagt cggtggcgca agcttgcaag
    57181 cagattcatt cttgtcattg ttagacgctg taaaataatt gttgaaaatt tgcgaaaaat
    57241 gcaagaaatg tttgcactaa tgagcaaaat taactaaaat cgtatatgag ggaaatccct
    57301 taggaaaaaa caaactcaaa ggagagacaa aacatgtcaa ttattactga tgtttacgct
    57361 cgcgagatct tagactcacg tggtaaccca acaatcgaag tagaagtata cacagaaagc
    57421 ggagcttttg gccgcggaat ggttccttca ggtgcttcaa ctggtgaata cgaagcagtt
    57481 gaattacgtg acggagacaa agctcgttat ggcggtaaag gtgtaactaa agctgtcgac
    57541 aacgtaaaca acatcattgc tgaagcaatc attggctacg atgttcgtga ccaaatggct
    57601 atcgacaaag ctatgatcgc tttagacgga actcctaaca aaggtaaatt aggtgcgaac
    57661 gctattttag gtgtttctat cgctgttgca cgtgcagctg ctgactacct agaagtacct
    57721 ttataccact acctaggcgg attcaacaca aaagtattgc caacaccaat gatgaacatc
    57781 atcaacggcg gatcacatgc tgataactca atcgacttcc aagaattcat gatcatgcct
    57841 gttggcgctc caacattcaa agaagctcta cgtatgggtg ctgaagtatt ccacgcatta
    57901 gcttcaatct tgaaagctcg cggattagca acttctgttg gtgacgaagg tggatttgct
    57961 ccaaaccttg gttcaaacga agaaggtttt gaagtaatca tcgaagcaat cgaaaaagct
    58021 ggctatgtac ctggtaaaga cgttgttctt gctatggatg ctgcttcttc agaattctac
    58081 gacaaagaaa aaggtgttta cgtactagct gattcaggcg aaggcgaaaa aacaactgac
    58141 gaaatgatca aattctacga agaattagtt tctaaatatc caatcatctc aatcgaagac
    58201 ggattagacg aaaacgactg ggacggattc aagaaattaa ctgacgtatt aggcgacaaa
    58261 gttcaattag ttggtgacga cttgttcgta acaaacactc aaaaattatc tgaaggtatc
    58321 gaaaaaggaa ttgctaactc aatccttatc aaagtgaacc aaatcggtac attaacagaa
    58381 actttcgaag ctatcgaaat ggctaaagaa gctggctaca cagcagttgt atctcaccgt
    58441 tctggtgaaa cagaagattc aacaatctct gatatcgctg ttgcaacaaa cgctggccaa
    58501 atcaaaactg gttctctatc acgtactgac cgtattgcta aatacaacca attactacgt
    58561 atcgaagacc aacttggtga agttgcagaa tacaaaggtt tgaaatcttt ctacaactta
    58621 aaaaacaaat aattcttttc taatgaagat acatgatcga ctattgaaag atagtcgagt
    58681 atgatcactt agttaagtaa tcgaactctc tacttgctga aatatcagca ggtagagagt
    58741 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
    58801 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
    58861 gtagaatagg aatctatgag caagtcctct cgtataaata aaataggagg gaagtagcag
    58921 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
    58981 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
    59041 gaagaaaaaa tcgcgatttt acaagagatc atccgaatta aatcagtgaa tggaaacgaa
    59101 ggtgaagtag cagcctattt gaataagctt ttggcaaagc atgacataac aggagagatt
    59161 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
    59221 aaagtgttag gcctatctgg gcatatggat gtcgtggcgg ctggtgatga atcgtcttgg
    59281 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
    59341 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
    59401 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
    59461 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
    59521 gagccaacta attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
    59581 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
    59641 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
    59701 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
    59761 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
    59821 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
    59881 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
    59941 cctttgattc attgcattca gcaacaattc agccagccat tacctttagt tggagcggca
    60001 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
    60061 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
    60121 ttggatatga ttgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
    60181 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
    60241 gcctattttt ctttttgaat atatcaagcg ttgtggcaac atagtgatga ctaatgaaac
    60301 aacgacgata gacaacaaac gatcaaggta gtctgtacct gcctgtacca agatcacact
    60361 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
    60421 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
    60481 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
    60541 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
    60601 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
    60661 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
    60721 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
    60781 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
    60841 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
    60901 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
    60961 aacgatcaac caatgtttgt ccacgactta ttccttgagt ttccacagca gtgaagcttt
    61021 tgaaaccttc acagatggcc ccctcgataa aataagcaac agctaacgga tcattgatga
    61081 cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga gtgatcgctt
    61141 ttagatactc tccctcttca ggattcattt gacagcagta ttcgaggata gtcggtgtca
    61201 agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg agattctcga
    61261 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggcgaacagt
    61321 taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt ttcccaatat
    61381 ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca atgactgatg
    61441 tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga ggttctgcct
    61501 gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca tgagtgtctt
    61561 gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca ggaatgtcta
    61621 aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct gcagggacat
    61681 tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca gctaatgata
    61741 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc attatttcca
    61801 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
    61861 acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc gaatgtaaat
    61921 gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa tgaaaaagaa
    61981 atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg gataatagtt
    62041 agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt tcattcttat
    62101 tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa agaaattagt
    62161 aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc gtttgatgga
    62221 ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag agtgtgaaag
    62281 cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga aactagctgg
    62341 tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac atgattaccg
    62401 aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
    62461 cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca tcggaatggg
    62521 atggctagat cggctagatt taattgcctg gatccaactc atcttagatg aacagccgaa
    62581 tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga tggcaagtgg
    62641 tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg tttctgttta
    62701 tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa tggtcatgag
    62761 atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag catcagctac
    62821 gagacagctt ggcagcaacc atctgccttt attggtcatt catggtgaaa gagatcattt
    62881 tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata aagcactgct
    62941 gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa cctattggac
    63001 agtgattttt tcatttattg aacaaagaat aacgatgaat taattttctc ttcttttttg
    63061 taagtaattc gaggataaat aattgtttat agatcaggag actgctaaaa taaggtagga
    63121 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa gaaaaaaatc
    63181 gtcttgcttt attcaatgat gaacaacaag agatcggaga aatgacatgg tcagatgcgg
    63241 gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt ggtcaaaagt
    63301 tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg aaaaagatca
    63361 ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat caagacgtct
    63421 tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta ttccatttat
    63481 atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc ttttgggaag
    63541 gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
    63601 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
    63661 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
    63721 caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca tatttatctt
    63781 cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt gcaaagagaa
    63841 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgagacgag
    63901 tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt ttgctgcttc
    63961 acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg gaaaaagcag
    64021 attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca tatgatattt
    64081 tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat tttttacagt
    64141 ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc tttgctgtac
    64201 gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct ttgtctcctg
    64261 taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg aagacagcag
    64321 gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta gctgaacaac
    64381 aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca gatatccaac
    64441 agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat ggcgtatttg
    64501 aaactgcacg caagttgagc cgtcagcgag tgacacttcg atggtatcct gaaagtggtc
    64561 atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg gaatttttag
    64621 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag agaaaattct
    64681 ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg ctgaaggttt
    64741 agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
    64801 agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa aaccaatctt
    64861 gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga ttgatcccga
    64921 agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg gagacaccgt
    64981 agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag aggggaaagt
    65041 agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg cttattcaga
    65101 agaagaaata caagaaacgg atctttacgg cgttgttatc ccgaaagata gaaagctcaa
    65161 ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa gtattgtgct
    65221 gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag ggataatcaa
    65281 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg tcgtggctaa
    65341 tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag ttccagatca
    65401 aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca ttgttacgat
    65461 tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat tagataacgg
    65521 taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac aaggcagtca
    65581 attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc gagtgattcc
    65641 gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg ttccaaggct
    65701 gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc atgatatttt
    65761 cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
    65821 agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt tccaagaaat
    65881 gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga tttcttttga
    65941 ggatcgcgaa gcaaaaatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
    66001 aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca atgaaacggt
    66061 agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg aacagccaaa
    66121 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
    66181 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
    66241 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
    66301 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
    66361 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
    66421 aagtgaaagc aatcaaagtt cgtgggctac ggaacttcct gaaattgcag atcatagttc
    66481 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
    66541 attcatgatg gacaaagtgg gggaagaatt cgatgggatc atcagctctg ttgtgaaatt
    66601 cggcctgttt atcgaactgc cgaatacagt agagggattg atccatatca atgaattgaa
    66661 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
    66721 gacttttaaa atcggacaga aagtgcgagt aaaagtagtc aaagctgatc cagatgaacg
    66781 agcagtagat tttgaacttg tttccgcaga agaggttaca gcgttggaac ggccaaaaac
    66841 acgaaatgga aaatcaggaa aacgcgaagg aaaacaagca ggaaaacgcc cagaaagaaa
    66901 tggcaagaag aacaaacgat atagcgatag taagcaaacg ttcggtaaga aaaaagggaa
    66961 gaagccattt tacaaaggtg ttaaaaagaa gaaaaagaaa aaataaaatg agttggagga
    67021 aacagctatg ccaaaaggcg agggaaaatt aattgcacaa aacaagaaag ctcgccatga
    67081 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaaccg agatcaagtc
    67141 gatacgaaac agccggatca atctaaaaga tggatttatt cgcgtccgca acggagaagc
    67201 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
    67261 gttgcgcacg agaaagttat tattacacaa aaaacaaatc atccggcttg aaaatgaatt
    67321 gaaaaatact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
    67381 ggtattgatt ggtctggcga aagggaaaaa atcttatgat aaacgggaag atttgaaacg
    67441 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
    67501 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
    67561 atctgataaa acatgattat cataggtttt ttcttagatc aggagcaccg tgtataaaga
    67621 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
    67681 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
    67741 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
    67801 aacgctcgct aacgttccac attggaccaa tttggtttga tggtacagtt tgtataatgg
    67861 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
    67921 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
    67981 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
    68041 ttttatttgt ttttgttgca aacaacatcg gtttagtaac aaaaatcgtt ttacctaacg
    68101 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
    68161 taatgatctt acttactcac ttatttgggg tgaaaaagtt aggcttcaaa ggatatttcg
    68221 taaattcgtt cttgaaacca tatagtttta tgtttccgat gaaactgatt gaagaattta
    68281 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
    68341 taaccttgat tgcaaaaatg gtaataagct taggctggtt ctcattgccg ttagcaattc
    68401 cattagagat ggtctggatc gcattctcct tgtttatcgg aagcatccaa gcatttgtct
    68461 tcgttacttt atctatggtt tatatgagcc ataagattga agtggaagaa taatattcgt
    68521 tttaaaaaca aactactatt tatctaattt aggaggaaaa caattatgaa ttatatcgca
    68581 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
    68641 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
    68701 atgtttatcg gggtagctct agtcgaagct gttcctatct taggggtagt tgtcgccttg
    68761 attctagttt ttggtgttta attgaatcag tattaaggca gaaacaattt ggtttgttac
    68821 tgctttttta catgaaaact agaagtactt caaagaaagg aaggctcgcc tatgctgaat
    68881 caattggcaa ttgcagaagt tggcaatgcg atgttaggaa atgtcatcgt tgtcagcggc
    68941 tcatttttga tactactggc tctcttgaag cattttgctt ggggaccaat cagcgatatt
    69001 ttgaaaaaac gtgaagacaa gatcgccaat gatttggatt ctgcagaaca atctcgcatc
    69061 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    69121 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    69181 gatgctcaag aagaagcagc tcgtctaaaa agtaaagccc aagctgatat cactgtagaa
    69241 cgtgattcag cgctgaactc tgtaaaagac gatgttgcag aactatctct tcaaatcgcg
    69301 gaaaaaatct tgaacaaaga attatctcca gaaatgcacg aatcattaat caatcaatat
    69361 atcgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    69421 gaaagcttta tttgaactgg cgatcgattc aaataaagca gaagaaattt atcaagatct
    69481 cgtaagctta cgtcaaatct atgaacaagt acctggatta ggtaatatgt taagtgatgt
    69541 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgttagtg gctatgatgg
    69601 aatcgttaaa aactttttag aagttgttta tgcgtataac cgaatggatg atcttttgtt
    69661 catgatcgaa gaatatgaac gtcgatatga tgaacatcaa ggtttgctgt tgggaagtgt
    69721 cacaacagca gttccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    69781 aacaatgaac tatcaatcag tcgaattaaa acaaatcgtt gattcttcta ttattggtgg
    69841 cgcgattgtt gaagctaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    69901 tatgcgtaat caattaaata gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    69961 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    70021 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    70081 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    70141 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    70201 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgattg
    70261 gacgggtagt taatccgcta ggtcagccaa ttgacggact aggtgaaatc gttactgaca
    70321 aagctcgtcc agtagaagca atggcaccag gcgttatgca acgtaaatct gttaacgaac
    70381 caatgcaaac aggtctaaaa gcaatcgacg ctcttgtacc aatcggacgc ggccaacgtg
    70441 aattagttat cggtgaccgt aaaacaggga aaacttccat tgcgatcgat acgatcatca
    70501 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    70561 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    70621 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    70681 tgggtgaaga gttcatgtac aacgggaaac atgtcttgat catttttgat gatctttcaa
    70741 aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca ggtcgtgaag
    70801 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    70861 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    70921 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    70981 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    71041 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    71101 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    71161 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    71221 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    71281 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    71341 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    71401 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    71461 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    71521 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    71581 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    71641 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    71701 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgttgctc
    71761 agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg tttagtcggt
    71821 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    71881 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    71941 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    72001 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    72061 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    72121 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    72181 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    72241 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    72301 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa ccgtgcaaga
    72361 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    72421 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    72481 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    72541 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    72601 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    72661 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    72721 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    72781 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    72841 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    72901 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    72961 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    73021 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    73081 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    73141 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    73201 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    73261 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    73321 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    73381 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    73441 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    73501 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    73561 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    73621 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    73681 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    73741 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    73801 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    73861 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    73921 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    73981 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    74041 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    74101 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    74161 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    74221 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    74281 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    74341 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    74401 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg gaggttgtga
    74461 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    74521 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    74581 ctgcgacaag actttt
//
LOCUS       DACEDD010000008        69865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEDD010000008 DACEDD010000000
VERSION     DACEDD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.008"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..1188)
                     /locus_tag="IWO45_000598"
     CDS             complement(37..1188)
                     /locus_tag="IWO45_000598"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0332099.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="IWO45_000599"
     CDS             complement(1201..1638)
                     /locus_tag="IWO45_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0332100.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="IWO45_000600"
     CDS             complement(1653..3419)
                     /locus_tag="IWO45_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0332101.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="IWO45_000601"
     CDS             3683..4360
                     /locus_tag="IWO45_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332102.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="IWO45_000602"
     CDS             4363..5490
                     /locus_tag="IWO45_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332103.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IWO45_000603"
     CDS             complement(5690..6130)
                     /locus_tag="IWO45_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0332104.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IWO45_000604"
     CDS             complement(6141..8174)
                     /locus_tag="IWO45_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0332105.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IWO45_000605"
     CDS             complement(8176..9879)
                     /locus_tag="IWO45_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0332106.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IWO45_000606"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IWO45_000606"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0332107.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IWO45_000607"
     CDS             complement(10962..11987)
                     /locus_tag="IWO45_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0332108.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IWO45_000608"
     CDS             complement(12196..13065)
                     /locus_tag="IWO45_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0332109.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IWO45_000609"
     CDS             complement(13524..15416)
                     /locus_tag="IWO45_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332110.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IWO45_000610"
     CDS             complement(15413..17140)
                     /locus_tag="IWO45_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332111.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IWO45_000611"
     CDS             complement(17156..17806)
                     /locus_tag="IWO45_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0332112.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IWO45_000612"
     CDS             18010..18306
                     /locus_tag="IWO45_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0332113.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="IWO45_000613"
     CDS             18478..19098
                     /locus_tag="IWO45_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0332114.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IWO45_000614"
     CDS             complement(19190..20356)
                     /locus_tag="IWO45_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0332115.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IWO45_000615"
     CDS             complement(20368..20721)
                     /locus_tag="IWO45_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0332116.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IWO45_000616"
     CDS             20849..21871
                     /locus_tag="IWO45_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0332117.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22310..22744)
                     /locus_tag="IWO45_000617"
     CDS             complement(22310..22744)
                     /locus_tag="IWO45_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332118.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="IWO45_000618"
     CDS             complement(22854..24713)
                     /locus_tag="IWO45_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0332119.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="IWO45_000619"
     CDS             complement(24831..25991)
                     /locus_tag="IWO45_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0332120.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IWO45_000620"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IWO45_000620"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0332121.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="IWO45_000621"
     CDS             complement(27738..28436)
                     /locus_tag="IWO45_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0332122.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="IWO45_000622"
     CDS             complement(28393..30054)
                     /locus_tag="IWO45_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0332123.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="IWO45_000623"
     CDS             30272..31672
                     /locus_tag="IWO45_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0332124.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="IWO45_000624"
     CDS             complement(31924..33003)
                     /locus_tag="IWO45_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0332125.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="IWO45_000625"
     CDS             complement(33089..34048)
                     /locus_tag="IWO45_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0332126.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35583)
                     /locus_tag="IWO45_000626"
     CDS             complement(34060..35583)
                     /locus_tag="IWO45_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0332127.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="IWO45_000627"
     CDS             complement(35593..36156)
                     /locus_tag="IWO45_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0332128.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="IWO45_000628"
     CDS             complement(36162..37025)
                     /locus_tag="IWO45_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0332129.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="IWO45_000629"
     CDS             complement(37039..37935)
                     /locus_tag="IWO45_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0332130.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39381)
                     /locus_tag="IWO45_000630"
     CDS             complement(38110..39381)
                     /locus_tag="IWO45_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0332131.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="IWO45_000631"
     CDS             complement(39371..40888)
                     /locus_tag="IWO45_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0332132.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="IWO45_000632"
     CDS             41086..41976
                     /locus_tag="IWO45_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0332133.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IWO45_000633"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IWO45_000633"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0332134.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="IWO45_000634"
     CDS             complement(42885..44357)
                     /locus_tag="IWO45_000634"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0332135.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="IWO45_000635"
     CDS             complement(44994..45356)
                     /locus_tag="IWO45_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0332136.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="IWO45_000636"
     CDS             45579..46331
                     /locus_tag="IWO45_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0332137.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="IWO45_000637"
     CDS             complement(46471..47394)
                     /locus_tag="IWO45_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0332138.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="IWO45_000638"
     CDS             complement(47409..47681)
                     /locus_tag="IWO45_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332139.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(48151..49776)
                     /gene="groL"
                     /locus_tag="IWO45_000639"
     CDS             complement(48151..49776)
                     /gene="groL"
                     /locus_tag="IWO45_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0332140.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(49827..50111)
                     /locus_tag="IWO45_000640"
     CDS             complement(49827..50111)
                     /locus_tag="IWO45_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0332141.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            50336..50998
                     /locus_tag="IWO45_000641"
     CDS             50336..50998
                     /locus_tag="IWO45_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0332142.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(51074..52366)
                     /locus_tag="IWO45_000642"
     CDS             complement(51074..52366)
                     /locus_tag="IWO45_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0332143.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            52536..53165
                     /locus_tag="IWO45_000643"
     CDS             52536..53165
                     /locus_tag="IWO45_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0332144.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(53268..54077)
                     /locus_tag="IWO45_000644"
     CDS             complement(53268..54077)
                     /locus_tag="IWO45_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0332145.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(54132..55001)
                     /locus_tag="IWO45_000645"
     CDS             complement(54132..55001)
                     /locus_tag="IWO45_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332146.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(55002..56318)
                     /locus_tag="IWO45_000646"
     CDS             complement(55002..56318)
                     /locus_tag="IWO45_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332147.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(56315..58150)
                     /gene="walK"
                     /locus_tag="IWO45_000647"
     CDS             complement(56315..58150)
                     /gene="walK"
                     /locus_tag="IWO45_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0332148.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(58155..58859)
                     /locus_tag="IWO45_000648"
     CDS             complement(58155..58859)
                     /locus_tag="IWO45_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0332149.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(59049..59909)
                     /locus_tag="IWO45_000649"
     CDS             complement(59049..59909)
                     /locus_tag="IWO45_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0332150.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(59896..60465)
                     /locus_tag="IWO45_000650"
     CDS             complement(59896..60465)
                     /locus_tag="IWO45_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0332151.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            60846..60918
                     /locus_tag="IWO45_000651"
     tRNA            60846..60918
                     /locus_tag="IWO45_000651"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60879..60881,aa:Lys,seq:ctt)
     gene            complement(61414..61800)
                     /locus_tag="IWO45_000652"
     CDS             complement(61414..61800)
                     /locus_tag="IWO45_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0332152.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(61797..62318)
                     /locus_tag="IWO45_000653"
     CDS             complement(61797..62318)
                     /locus_tag="IWO45_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0332153.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(62320..63354)
                     /gene="sppA"
                     /locus_tag="IWO45_000654"
     CDS             complement(62320..63354)
                     /gene="sppA"
                     /locus_tag="IWO45_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0332154.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(63387..64802)
                     /locus_tag="IWO45_000655"
     CDS             complement(63387..64802)
                     /locus_tag="IWO45_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0332155.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(64771..66924)
                     /locus_tag="IWO45_000656"
     CDS             complement(64771..66924)
                     /locus_tag="IWO45_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0332156.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            67182..67418
                     /locus_tag="IWO45_000657"
     CDS             67182..67418
                     /locus_tag="IWO45_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0332157.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            67437..67652
                     /locus_tag="IWO45_000658"
     CDS             67437..67652
                     /locus_tag="IWO45_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0332158.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            67790..67948
                     /locus_tag="IWO45_000659"
                     /pseudo
     CDS             67790..67948
                     /locus_tag="IWO45_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(67955..69148)
                     /locus_tag="IWO45_000660"
     CDS             complement(67955..69148)
                     /locus_tag="IWO45_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0332159.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     IKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEGQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTIMDVDFIHEVVVIDHQLLKSKEQGYYIETPKTKSGTRQVPLSK
                     ETIQAFQRVIKKRPKAKPFVIDGRSNFLFVNQKGKPKVAIDYSTLFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(69227..69430)
                     /locus_tag="IWO45_000661"
     CDS             complement(69227..69430)
                     /locus_tag="IWO45_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0332160.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(69430..>69865)
                     /locus_tag="IWO45_000662"
     CDS             complement(69430..>69865)
                     /locus_tag="IWO45_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0332161.1"
                     /translation="FAMTFKDRPLELGELAFGLLANNLRFVVPNRNESNKSRWKTCRF
                     WERFLGAVEVLKLQVPKLHNSLEETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGT
                     MLDKARYSNSLSSKLTAHLQRIDRTDLIPYIQYDTKHGKGGI"
BASE COUNT        20243 a        14462 c        11923 g        23237 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
    47881 tataactatt aaaaataact attttcatta taattacaca caaaaaagag cgtgagacaa
    47941 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacagcggaa gcagaagcaa
    48001 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaatttccg tgaatttctt
    48061 cttatttctt ggagttaaac gcttctgttc cagcctcttt ttatctgtct ttcactaatg
    48121 atcagttgta gataagcaag ttgctttttc ttacatcata ccgcccatca ttgatggatc
    48181 cattgcagga gctgctggcg ctgctggttc tggtttatct gcaacgactg cttctgtcgt
    48241 taataacaag gcagaaacgg aagcggcatt ttgcaaggca gaacgtgtta ctttagttgg
    48301 gtctacgata cctgcttcaa ccatgtttac ccattctcca gtagcagcat tgaaaccagt
    48361 gcctagttcc acgttcttca atttatcaac aatcactgat ccttcataac cagcgttttc
    48421 agcgatttga cgaattggtt cttctaaagc acgaacaacg atcttgatac ctgttgccac
    48481 gtctccttct gcttctacag cagaaacttt gctgataacg ttcactagag ctgtaccacc
    48541 acctgagacc attccttctt ctactgcggc acgtgtagca ttcaatgcat cttcaatgcg
    48601 taattttaat tctttcaatt ctgtttcagt agctgcacca actttaacaa cagctacacc
    48661 acctgctaat ttagctaagc gttcttgtaa tttttcgcgg tcaaaatcag aagttgtttc
    48721 agcaatttgg tttttgatca attgcacacg agcttcgatt gcttcttttt ctccagaacc
    48781 ttctacgatc gttgtattgt ctttatctac aacaacttta gaagcatttc ctaagttttc
    48841 gattgttgca tctttcaatt ctaaacctag atcgtcagta attactgtac cacctgtcaa
    48901 aatagcgata tcttcaagca ttgctttacg acgatcgccg aaaccaggag cttttacagc
    48961 tactacgttg aatgttccac ggattttgtt caatacaaga gttggcaatg cttcaccatc
    49021 tacatcgtca gcaataatca acaatggacg tgattgttgc aagatttgtt ctaacaatgg
    49081 cagaatatct tggatgttag agattttttt gtctgtgatc aagatatatg ggttttctag
    49141 aacagcttcc attttgtcgt tatctgttac catatattgt gataagtaac cacgatcaaa
    49201 ctgcatacct tctacaacat ctagttctgt ttcgatccct tttgattctt cgattgtgat
    49261 gacaccgtcg tttccaactt tttccattgc atcagcaatc aaatgtccaa ctttatcaga
    49321 acccgaagaa acagctgcta cttgtgcaat ggcttctttt gaatcaacaa cagttgagat
    49381 attatgcaat tcttcgactg ctgcttttgt tgccaattcg atcccgcgac gaatgcctag
    49441 aggattagca cctgcagtta cgttttttaa accttcgcga acgattgctt gtgtcagaac
    49501 agttgctgtc gttgtaccgt ctcctgcaat atcatttgtt tttgatgcta cttctgatac
    49561 aagttttgca cccatgtttt caaaatgatc ttctaattcg atttcttttg cgatcgtcac
    49621 accatcattt gtgatcaatg gagagccgta agatttttct agtacgacat ttcggccttt
    49681 aggtcctaat gttactttca ctgtatctgc taatttatct accccgcgta acatagctgc
    49741 gcgtgcgtct tctgcaaatt tcaattcttt tgccatgatt gattcacctc attgattatt
    49801 tttgattatt tttttatctt ccaaatttac tcaacaatcg ccatgatatc ttttcctgcg
    49861 acgatcagat attcttttcc ttcgtatttt acttctgtac cggcgtattt ttcgaacata
    49921 acttggtcgc ctgcttttac agcggcagga actttttcac cgttttctaa caaacggcct
    49981 tcaccgactg caacaactgt cccagtttgg ggtttctctt tagcagcaga agctaaaacg
    50041 attcctccga cagttttttc ttcttctttt gcgacttcga taatgacgcg atcacctaat
    50101 ggttttaaca cgataaatcc ctccgataac tcttttttta atctgttagc actcttaacc
    50161 ttcgagtgct aacctacatt tttaataata ctcattctcc ctcttttttg caagctttta
    50221 aatcaaagaa aagaaatttt attctgacag ctgcttattg aaattatgat ataattgaaa
    50281 tcaatcgata taagcttgcc ttgcatgctt gttatccctt aaaaggagga attacatgtc
    50341 tttaaaaaaa tatagcttcc tcagcatttt ttgctatggt ctaattttta tctcaccctt
    50401 gatcctagct tcgataggac ttgtaaaatc tacgtctgaa ttgatcacag caactgcagt
    50461 catgtatata ctaggcgccg ttgtcctagc tattttttat tttaagcaac gtgaaccctt
    50521 agcgatcgaa tcagcggtaa aaaaagcttc tgctccaaag attgttattt atggattggt
    50581 tggaatcttt gtcgcattga ttctgcaaag catcgctgtc atgttagaat ctgtattgtt
    50641 tggcaaagca accccttccg agaatacaca aaatatcatc cagatgatca tggaagcacc
    50701 cgcttttatt ttggcgacaa ctgttgctgg acccatcatg gaagagttcg tattccgccg
    50761 cagtatactg gggatcatca gtcgatattc aaatttttgg gtcggagcag tgatcagctc
    50821 tttgctgttt gcgtttgctc ataatgatgg tcatctgcta atctactttt tcctaggttt
    50881 cttcttcagc ctagagtaca aagcaactgg tcggatctgg acatcgatga tcacacacgt
    50941 aggaatgaac acattagttg ttcttgttca attagctgtc caaaaagggc tagtataaac
    51001 atgactataa atactcctgt catcctagat tggtgatttt cccaatctaa agcgacagga
    51061 gtatttttgt tttttagtcg ttcatctttt ctgctgctcc atcatttttt gtctgtcttt
    51121 cctttttgca gtttgcagaa tctatttttt tcaaaaatgg gaaataaatg aaaaaactaa
    51181 ttgacaacac gataccttgt aaaatcgctg caggcactcc gcctactaag aaacccgaga
    51241 ggatcggcgg tgttgtccaa gggacttgga ctgctgtaaa taaaggaacg aatccaaaat
    51301 ataaagcgga ataggtaatc aatcctgaaa cgaccggtgc gaaaataaaa ggaacagcca
    51361 tcatcgggtt catcacgatc ggtgtggaaa aaataattga ttcattgatg ttgaaaaagg
    51421 ctggtaaaag agcaagattt cctaattctc tgtatttctt cgatttcgca aaaaccgtca
    51481 taaaaaatac agcccctaac gtcaatccag cacccgtaac cgtcatgaac tgatcgagaa
    51541 attgctgagt aacgatatgt ccgccatttg ccaccgtcaa ctcttgtcct gaggctaaga
    51601 tttctgcatt tgctaatgaa ttcgcttgca agacgggact aaccaaccca ttgaccatcg
    51661 cagatccatg aagaccaaat aaccataaaa acggaacaaa aaaacctaat aaaagcacac
    51721 caaacaacgt gtctatgaac gattgaatgg gaagctggac atatgtccat actccttgaa
    51781 caacagtcat tctgaacaaa tgttgaaaaa ccgctgaacc gataagcgta aaagtgacta
    51841 taacaatgat tggaatcaat gaagtaaaag actgccaaac attttcaggt atcttctctc
    51901 tttgcttcca ccctttcgca agtcttacta aacgtgcata gccccatccg atgatgagcc
    51961 cagttgtaac tgctcctagt atcccttcag cacctgtcca gacagtatct atctgagtat
    52021 ttagtggctg aataagcagt agataagcag acaaggctgt tatgccgact ggtaagcgat
    52081 cgatgtcttc ttcttttgca taagcgtatg caatggcaaa aacgatccaa aaggctatca
    52141 catcaaatgt agcctggtat acttgtgtaa aatacataga aaatcctgtt ttgtctatcc
    52201 actgagcgat gtaatccaca ggaaaatgag caaataagaa aaacagcgag ccaacaataa
    52261 ttattgggat cgtgtaagtc attccattac gcaaagcagt taagggtcta agattcacaa
    52321 cagatgttat cattggtaaa aaggcacgat ctaaccactc tttcatttct tatctccttc
    52381 aacttctatt tttatcaatc agttttctta atttaactat acccaatgat cacgaaccat
    52441 gaaaggaatg cgcactgagc ttttcaaaaa tcggattctt tgacgaatat tcagcaactg
    52501 cttacaatgg acaggaataa aaaaggaggt tttttatgaa aattgcactc attggagcta
    52561 ctggccatgc aggttcattt attttagacg aagcattgtc cagagattta gacgtgacgg
    52621 caatcgtgcg tcatccagaa aaattgccat cagatgttcc gtttatacaa aaggatttat
    52681 ttgagttgac aactactgat cttgcatctt ttgatgttgt cgttgatgct ttcaacgctc
    52741 ccaaaggaaa agaagaaatg catcaaactt ctttgtccca tttgattgag atgcttgaag
    52801 gaaccgatac acgcttaatt gtcgttgggg gtgcctcttc gttattctta gatacagaaa
    52861 agacgactcg tatgattgac caagtaccta aaaatgcacc cttctatcca acagctttca
    52921 atatgtacga aggattgtta aaattgaaac aatctaaaaa tctacattgg acttatatca
    52981 gtccagcttc tttatttgat ccacatggca aacgtacagg tacttatttg ttaagtgatg
    53041 attatttaca aagaaacgct gctagcgaca gtacgatcag catggcagat tacgcgattg
    53101 cattagtaga tgagatactg gatgagaaac atgaaaagca gcacatcagt gtagtcagtc
    53161 gataatacgg ttattttttt aatatgctaa aaaaataaaa atagaagata aaaccaaccc
    53221 tgattgattt tatcttctgc tttttatatt tttattgatt gaactgttta aatcgaaaat
    53281 agctcagttg cttcatctgg atctgtatca tagatttcaa aatcatgtcc aacgccaaaa
    53341 tctttttctt tcaaaacatt ttccattgtt aaatgagcaa gttctttcat attgttttcc
    53401 ttgctaagat ggccaagata gattcgtttg gtccgattac cgatcacatc agtcatgaca
    53461 agtgcaccgt cttcgttgga aagatgtcct ctatctccta aaatacgctg ttttaaattc
    53521 cacggataag gtcccatgcg taacatctca agatcatgat tactttccat caaataacca
    53581 tcggcatttt caatgacacc tcgcatataa tcactgcaat atccggtgtc cgtcaacatg
    53641 acgaatgacc gattttcttt atggaaacga taaaattgcg gagccgctgc gtcatgggag
    53701 acgccgaaac tctcaatatc gatatcgccg aatgttctga ctttccccat ctcaaataga
    53761 tgtttttgtt ccactggtac atttccaatc agtggttcca tcgcttccca agtctgctga
    53821 ttggcaaaaa catccaaatg atacttacga gccaagacac ccacgccatg tatatgatct
    53881 cgatgttcat gcgtcacaag tattccatct aattggtccg gtgtccgatc aatttgagcc
    53941 atcaaggaag tgattttttt accgctaagt cctgcatcga ccaaaagccg ttttttttca
    54001 ctttcgatat aaagtgaatt acccgaactt ccacttgcta ggatactgat tttaaaagct
    54061 ggtttttctt cagccatttg actcctactt cccttcttac tttgtaaact tttttcatta
    54121 tactcgcttt tttattgatc ttccactttt ggtacagtat tgtttgtcac gatcgtattg
    54181 ctgaaagcgt tgacgctctc aatttggacc actttatcgc ttgtttcgat cgctacatgc
    54241 caaacaggaa catacacatt tttttcccgg actttcagaa tacaggaata agctagctga
    54301 cgccataaaa tttttgatcc tcttggtatt ttgttattga tgtacaatgt attgattgct
    54361 tcctcttccg tatacagttc cgtcttttcg cgcagctgct cgatatcaga taaatgcgtc
    54421 tgcatgtatt tcgtgatgct ataatcatta tcgttttttt ctaaagaaag aacgattttt
    54481 gctgtatcat cattgaatgg gatgccttca tactcttggc ttgcttggat ttctagtggt
    54541 tcttcttcta aatgagaaaa gtttttcgtg tacacataat cattaccgaa taaaatattg
    54601 tctttgttag ataaaaaatc ttccaaggaa ctactaattt tatctggatc gatcacgttc
    54661 ttttcagcat ctgaactgga taaagatttc gtcaaaacat caccgtctaa aaacacacca
    54721 cttgccagca agccgctttt accagccttg cgataattcg ctaatgaagt gctaagattg
    54781 gtctgctccg cgctaagata atagccttgc ttgttctcag acgaaatctt ccccgtgtaa
    54841 gtgatatcgt cgctagccag acgttgttgg attgattcag tctgattaga gcgaatgaca
    54901 ttgttttctt ggtataccgc gtcatgataa ctgctgaata aaaacacatt caaccctaaa
    54961 aaagcgacga aaaatatcca ttcgattttt ttgaaatcca attaattcgc ctccaatcca
    55021 ggtaattttt ccagaaggtc gttagctgaa taccagttat ctttatattt cacataccat
    55081 tcaggtaaga gatcaacgac acgattcgtt tctttgacat tttgccatgt atagccaacg
    55141 atcactgaac caatcttgtt gctatctgct cccttagctt ttaactgctg gatgatttca
    55201 ttcgtacttg caagttctac ttcttcgtct gaaggtatcg gcacttgaat cgtattcaaa
    55261 ctggtttgga tattcacttg cacagaacca ttcgtttcac cgccaacttc cacaccaatc
    55321 tgtcctctgc tgtctgtccc aaaaaccggg aagccttcca cgaatatccg ataatcgatc
    55381 gtattgtcac tgcggtcaaa atatctgagg ttccctaaag aactgcctaa tttgctaata
    55441 taggggaaac tttcagaaaa tacactagca ttttcttttt tctctggcag ctctccacga
    55501 aaatcaacta cttgcgtttc cgaatggata gacattgttt cattcccacc gtaaaaaatc
    55561 aattcattgc tttcatcatt atttttcacg ctatctggtt tctggaaaaa agcatttcta
    55621 aaaagtgtat agggttgctg tgacaggata taactatact tcttcagttt gatcgaatgt
    55681 ttcgtagcat attgcgtgtc gatcgtcgat ggttctgttg tcatttctgt ccattttgtg
    55741 tcttttgctg ttaattcttt attgatatct gtccaattca acgaaatatt tgcttcatat
    55801 acgagatggt tcgaataatt caaaaatcgg atttttttat ttccttgatc gaattgtatt
    55861 ctagtaaaat acatattttc gctttctttt gccaagttcg acacatctaa atctaaatca
    55921 aatgcttcct tgtattctct tagcaaaaaa ggcgcattat aggccaactc aaaaccagag
    55981 ttcagatttt tcacctgttc aaactgtgat ttgtctcctg aaactacttc agaaagcttt
    56041 ccaaatcttg ctttatcgat gatcaattgc aatcggtaca cgagattttc actgttggtt
    56101 tcttggatca ttcccgaatg gtgccatgta acatgaagcg gaagaaagac ttcggaagtt
    56161 tttcgattgc tttgagactc gatcatttga gtgtttgtct catctacgtt gattgaactt
    56221 tttcctgctg ggctcagcca gattgcataa gagaagtata tgcttaacgc aaccatcaaa
    56281 atgagtccta tacgaacgac tttttcagag attctcattc ccaccattcc tcctcataag
    56341 gttcatatgg cagcgagata taaaaagtcg acccttttcc ttccttgctc tctgcccaaa
    56401 tagcgcctcg atgcgcctta atcacttctc tagaaatagc taatccaagt cctgtcccac
    56461 cttgtgctct tgcacgtgct ttatctactc gatagaaccg ctcgaacact cggttcagat
    56521 cttttttagg aatccccaat ccttgatcgg tgatacttaa aatcacgtta ttatgcgttt
    56581 ctaataagcg acaagtaatc gttcccccat ctggtgaata cttgatcgcg ttattcatga
    56641 tattatcgac tacttggatg attttatctg tatctacttc gacccaaaga tctctttgcg
    56701 taaattcacg acggatctta taattttttt cctgatttcc caccatcata tcaaaccggt
    56761 caagaacaaa attcactaac tcgttgaaat tgacatattc taattgaagc tgggtattac
    56821 ctgtatccat gcgagataga tttaagaggt cattgatcat tcgaatcatt cggtctgttt
    56881 catctagcgt taccttcaaa aaatttggtg ctacttcctc gtctttccac gcaccttcgc
    56941 tcaatgcttc gatataacta cgcatacttg tcaacggtgt acgaagttca tgggaaacat
    57001 tagagacgaa ttcacgacgt tcccgttctg ttttttcttg ttctgtcaca tcatgcaaga
    57061 cagctactaa tccgctgata aaacctgatt cgcgacggat catagaaaag tctactcgca
    57121 agatcatctg atcactttcc aatgtactgg ttgaacggtc aagtaccatt tcgtttggat
    57181 cttctaataa tttccgcaac gtatattctt cttcgatatc taaaagtgtc aaaatcgatt
    57241 gtccgattgc ttcctcgttt ttgatatcta gcaaggaaac agccatatca ttgatcgtaa
    57301 tgactttacc cctgcgatct gtcgcaatga cgccgtctgt catatgggat agcacgctat
    57361 ccaaacgatt gcgttcagct tccattgtat cttgagcttc ttcaatacgt tcagctaact
    57421 gattgaaggt atcagccagc tgtccaagtt catcttgtcc atgcactttg actttccgac
    57481 tgtagtctcc tctagcgatt cgaatggctt gttctctcat ttcaccgatc ggctgtgtaa
    57541 tcgaacgagc aactaaaaca gatacgatca ttgaaataaa tgccgcaatt agtgaggcag
    57601 taaagaaaat gactgctgtg ttattgattt ccgaatattt ttgttcgata ttacttttga
    57661 cgtaaagagc cccgataacg gcatcgccgg tcggggacag gatcggttga acattgatat
    57721 tcacccgttt atcgtcgtta tctaaagcaa cataacgttt agtcgtgaaa tcattgatat
    57781 caacataatc atttttcttt cccacagcgc tttgttcatt gacatcggtt gttcctcgga
    57841 tgatcccttt ttcgtcaacg acccgtattt cgatgatatc gctagtcgca ctgttttcaa
    57901 taaatctttt gatattcgca tttacctctt catcatctgc attatctttt cgattcaatt
    57961 ccgggctgat tgttgttgct aatgattcca ccgtttgatt catatcttta ataaatgtat
    58021 tgatcgtgga acgttctaaa ccacgtataa agtaggcacc aataatttcg atcgagatca
    58081 ataaaatcag aataaaggta atagcgatct taaaattgac tgatcgaaaa aagcggactt
    58141 ttcttttcat ccaattactc ctgttctggg ttacgcaaat agtaaccgac accacgacgt
    58201 gtcactaaat aatttggatg gcttggatta tcttcgatct tttcacgcaa acgacgtaca
    58261 gttacatcca ctgttcgtac atctccaaaa taatcataac cccaaaccgt ttgtaataaa
    58321 tgttctcttg tcatcacttg tccaaggtgc ttcgccaagt aaaagagtag ttcgaattca
    58381 cgatgtgtta actcgattgt ttctcctcgt ttcgtcacca tgtaagcttc tggatggatc
    58441 gtcaaatcac caatcgtcaa ttccgcaggt gctgcctctt ctggttcctt cgcagccgtg
    58501 gcaccgcgac gaagatttgc tttgacacgt gcaactaatt ctcggttgga aaatggcttg
    58561 gtcacgtaat catcggcgcc taactctaag cctaggactt tatcaatttc agaatctttg
    58621 gctgttacca taatgatcgg catatcataa gttttgcgta cctcacgagc aacttctaag
    58681 ccgtccattt taggtagcat caaatcaagt aaaatcaaat caggttctac ctcagcaact
    58741 ttttccaagg cttcttcccc atcatatgca gtataaacgt catagccttc tttggctaaa
    58801 ttgaatttga cgatatccga aatcggcttc tcatcatcga caactaaaac ttttttcata
    58861 aagagccacc tcttatatat tattgtcata ccttctcatt ataccgtatt tctgcttttg
    58921 tttcatcttt gcctataaaa caaaagagaa aattaaaatg aaagtgcaaa atactttatt
    58981 attttttgtt ctaacttcaa taaaaaagga aaacactgat tttcacctca gtgtttcctt
    59041 tttttgattt atccttctaa aacttgtaca ccgacaccca ataatcccgg accagtgtgg
    59101 actactaatg ccggagaaat ctggccaaag tagatttggt ttgcttgtgg gaactctctt
    59161 ttaagtctcg cttccatttc aattgcttct tctttcgcat ccccatgggc aacagctaag
    59221 ttgaagacct ttgcattacc gattttttct ttgacgtaag caaccgtttt atccaaactt
    59281 tttttacgtc ccctagcttt tcctactgtg taataaatcc cttcttcatt acatgagatg
    59341 attgggttaa gctttaaagc ggttcctaca atagaagcaa ctaaaccgat ccgtccacct
    59401 ttttgaagat attccaatgt cgcaacatta aagaaaactt ttgttctttc gactaactca
    59461 ttcagattac tgatcagttg atcccattct actccttctt ccagccattt tgctgcttgg
    59521 atcgcagaaa aacctgcacc aataccaata tttttcgtat ctagtacata tgcttccaat
    59581 tcttcttgct gttgtgcaat taaacgaacc acattatatg tcccgctcaa tccactggaa
    59641 attgttaccg caagtacctt ttcataaccg tcttttttga tttgctcaaa gatttctgtg
    59701 atcgactcac catccggcaa agatgtacca gggatttcct ctggcaaacg ttgatagatc
    59761 tcttcagggg taatgtcaat tttatctgta tatacctgat ctttataaat aattttcaat
    59821 gggatcatat acatctcgta tttttttatc aattcttctg gaacatccgt acctgaatct
    59881 actaaaattg cgattttatt gtttttcatt tgttttctcc tcttttaaat attcggtttc
    59941 tagaacaatc gttttttccg caagaagttt cattgcaaac gataaagttg ccgctcttac
    60001 agcaatatat tctacagggg ttgcctggtc gaatttttgc ggatgagatt taccttgtgc
    60061 taattggctg accatccaca cagctgcttc ttgttcatcg caaaatagat tataggcttc
    60121 acgaattcct actgtttttc cttggaacaa tatcccttct ttgatttcag gaatagtcaa
    60181 tacttgtttc aacgtagtga ttgcaagtaa aaatgctacg tgttctttgt tatatctttt
    60241 tttaactggt gctggtatca aacctagttt aacgtaattg tttaccatag aagaggtaag
    60301 caatggatgc ttttctcctt gtatcacagg tgaaagataa cgatcaacta acgttctgac
    60361 ttggtccata taaagttcta agtcagggag ttcttcccag tgaggcagac ggaatgtttc
    60421 tagttccttc ccccactcca ttaattgctg acgcattttt tccatcataa ccgctccttt
    60481 tttcatttca taaacatgat atcagactta tgataaacat tcaatctagt tgctttaact
    60541 aggtgatttg attagcggga ttccatgaaa acaaaaaaca agaaggctaa gccttcttgt
    60601 cttttatcgt taagaacagg agacccgccg gggagacggc gggaaaggag taaaaaatga
    60661 aaaaagtgtt agggtttgtt ctcttgcaag aactttgttt gttggtatgc ttatataata
    60721 gcgagcaaat gtgaataatc tatgaatgat ttcctataag gaagtaaaga tttttctgat
    60781 tttctttaaa tgtagtcttg cattttaaaa ttcttagtgc tactattatt aagtactttt
    60841 tgtacgggcc gttagctcag ttggtagagc agctgactct taatcagcgg gtcgcgggtt
    60901 cgagcccctc acggcccatt gggtgccaaa cccacggaac aggtaaatgt tggtgcgaaa
    60961 gcatttcgaa aacatttacg ctgttctttt tttatttttc aaaataacta gggcttttta
    61021 tttttaactc aaagtccact gtagcccttt ggactttgag ttaaaaaaga agcataatta
    61081 tgaacagtaa agtttctaat ataacaataa ttcgagaagc tttgcaaaat catgctgcca
    61141 atgaactctc taaacatata ggattaaatc taagtactat aaaaggttaa aaagtgaaga
    61201 atatttaata gaaaaactaa atttacttgc tgctttcccc ttttccatta atacaacaat
    61261 cagagagtgt ctttttttcg aaaaaattag aataaataaa agacacgtag tttttttatc
    61321 tacatgtctt ccatcgacta caagttatca tttctcaaaa cgtatttagc ttttaattga
    61381 ttaatcgttt cacgttgtct acacattgcc taattaagat tttcttatta gaagcatcaa
    61441 tcccgcaata atacctaaaa ctccaggaat tacccacaat aatgttaaaa gaaaaaatgg
    61501 aataatagat aaaatgagta aactaatacc atagaccact gttttttgat tttttcttac
    61561 taaaaaacct attatgaaaa tcattatcga aaaaactagg gtaattcctc caaataaaac
    61621 aacattcccg cttagattga ctaacatatc tatatcctta ctggttagtt ctgtagtatt
    61681 taaagtagtt gaatcaatag aagatagaaa cataccaccc atcaatgcta aaagactaca
    61741 taaaatccct gttattccac taattaaaat caaaataaac tctgtttttc tcttattcat
    61801 ttaattctct ccagattaag tgttttagaa ttagaaattt tttgaccata gttaatagct
    61861 tctaatgaac gaataacaac cactgacgtg ttagaaaaat aatccccaat atgttgtttc
    61921 ttgggcgtga aagcagccac caaataccca atttgaaata caaaattttt taatataaac
    61981 ctacatatac actcacgaat aaagatagtt tgccaactta gctctttttc ctcaaagctc
    62041 actactctta tcccaaaaat cattttccca attgtttgtc ctgaattcag cttagtcaaa
    62101 agtacaaaat aggttaaata aatagctaat gacgctatac cataaaaagt aataatggat
    62161 ttgtcagatt ccagtcccat gattttgtaa aagagaccta tagtagcatg ggtgatgatg
    62221 ctaatacaaa ttaaatcaat aagaaaagca aaaaatcgta accaaaatcc cgcaaataca
    62281 taattaggca aagtttcaat gccactcttc ttattcattt tatagccctc catagtaata
    62341 cattggtttt ggagtatcag aaaatccaag tttttcaaaa actgaaatta cacgatctga
    62401 tgtagttgcc tttagccctt gccattcagc aatcttatta cctaaccaag aagaagcaaa
    62461 gccagttgtg tcatttttat attctataac cgtcgcatca gttaatttct tctctttttt
    62521 tagggaatct aatgcatctt cagagtaacc aatttgatcc accaacccat tttctactgc
    62581 ttggttacca tcataaattc ttccatccgc aagttctttc actgcattag tatccatatt
    62641 tcttcctttt gctacaacat tgacaaaacg atcataagca ctcatcacaa attcctgtat
    62701 aactttattc tcttcctctg aaggtttgtg ttgaggtgac atcatatctt ttaatgcacc
    62761 acttttatac gtcgcatcag ttacgccaag tttttctaat aagccagaat aatttaaacc
    62821 agaaataatg acaccaattg atccagttgt tgtttcttcc gtagcaaata ttttatctga
    62881 accagcagaa atgtagtacc ctccacttgc cgctgtattt ttaaatgcag tatagatagg
    62941 aatatctagc tttttaattt ttgccatttc tttagcaatc tcagcacttt catatattcc
    63001 tcccccaggt gaatttactt ctaataacac tcccttaacc gctttgtctt cttgaatttt
    63061 ctttagctgt gttaaaaaat tctgatggtt atattgttcc ctactaaaaa gattgctttc
    63121 acctgtatct gcaataactc cattgacgga tagtttgaca atctttttat ttgatgcgcc
    63181 ttcttctaac gtttcttctg tgatttcgtt agatccgtaa aaaatcttat ttattcctgt
    63241 tagggttgta tttgatgctt cctctttatc ttttcctgga attagcgcga cgataatgga
    63301 tagtaacaat aatccgcacg ctactcccac agcaatccat cttcttttat tcatataaac
    63361 tcctcctata ttcttagtaa aaataattat tttttattta gaaacacatc gacaatttgt
    63421 tgccaatatg ttttttctcc tacaattaaa gacaactctc cagttaatcc ataacgaaac
    63481 ggaaacgatt gagttgtttt taacgttcct ttcactgcat aaaaaacacc tttctcagtg
    63541 gcagtagaat tttctgaaat ttcatctaaa gttccgtcga tcgtaatcgg agccccacct
    63601 ttttgatcca atttaaaatg aaccttcatc ccttctttaa tataggtact ttcattcgca
    63661 ggtaaaagtg ctgtaaattt catttttgat ttttcgtgga tcggataaat ttctgcaagc
    63721 actgttcctt tggctagttc tgcttgtccc tttgtttctt catttaaatg aaccgttcca
    63781 gatacaggag cttttagtat tccttttgaa agttgatcgt ccaaactttt gataactgac
    63841 tggttcttct cttgtttttc atttagctct gtaactgttt gttttacagt tgttattgct
    63901 tgttctagct cttgctttcc tttttccgtt tgactaattc tgtcattctc agaagtagtt
    63961 ggtttaacta atttagcttt ttctgactga tactgttcta cttctttttt tagttgctca
    64021 atttgttcat ttatgcttgc tgaaattgtc agtttcactt gatttttttg atcatcagaa
    64081 acattattta gttgttcttg ccaattctca tagtttgcca tcatttcttt tgaaaaatct
    64141 tttaatgatt gattattact ccaagctacc tgtacttgtt gccattcttg taatttacta
    64201 tctgaactct caatttgttt ttctagttgt ctttttgtct tttcgtatac ttctaattgc
    64261 ttttgatcat ttaattcact ttgcttcaaa gcgtagcgga gtgattcttt ttctgacaac
    64321 atgctcttta attgattact gtagccaaaa ctgtcatccg ttgaaaacaa attttttcct
    64381 ttacttatac tttcaataaa tagctgagcc atctttattt gttgagtaat acgttggttt
    64441 tcttgttcta tctgagcctt ttcattctct attaaagaac aatccaatct aaccaacaca
    64501 tcgtccttat gaacaaattg attttctttt aggaagtttt ccattatttt agcttctaat
    64561 ggcacttgta acttctctat tttttcgggg ttcaattgag ccgttgtccg aataacaacc
    64621 tcttttttag caaaggttaa aaacaatcca ataatacata aaaaaagaat tacagggtat
    64681 aacacccaac ggtagaagct atgatgttgt ttagtataga tactagaatg ttcgaaccaa
    64741 ttattattct gcataaattg tttccctctc ctagatttcc cataattttt gatagatacc
    64801 atcggcaaaa cgaagttcat cgtgcgttcc tttttctacc aaatgccctt catggagaac
    64861 taaaatttga tcacatgctt ttgctattgg taaatgatgc gcaataaaaa taatcgtttt
    64921 ttcttttaaa ttgaggagat aattcataat tgcatgttct aacaaggtat ctaatccact
    64981 agttgcttca tctaaaatta atatgtccgg attttttaat aaagcccgtg ctaaggctaa
    65041 tcgttgtcgt tgccctcctg aaagattatt tccaccttct tccaacaatg tctcgtaacg
    65101 taatggctgt tgattaataa attcatttag ttggactatc tcacaaactt ctattatttg
    65161 ttcgaaactt ggtgggctat ctaaaccgaa tataagattc tcaatgattg ttccactaaa
    65221 gaaaaatgac tcttgaggca aatatgtcac tcgatcgcgt aatatctgaa aaggaatgtc
    65281 taagtgatta atgtttccat aatggatcga tccttctgat gcttcataaa aacgaacaaa
    65341 taactttgca agagttgatt ttccagaacc actaactcca actatagccg ttttactgtg
    65401 cgaaggaatt gtacatgaaa tttcgttcaa cgttggggcc ttcatgctat acgagaatga
    65461 aacgtttttt acagtgatat cttgttgaaa taattgttca gaaagcatat attcaaaaga
    65521 ctggttcgat ttttctgaat caatcgcgaa aatttcattt aaacgattat ttgcaacttg
    65581 agccgtttgc attttcactt gtaaattaat aatgttttgt aaaggatctg tcaaaaatac
    65641 tagtaaagca ttgtatgtga taagttgacc tagactaatt ctattatcca taacaaaata
    65701 agaacctagc cataaaatta atgcactgct aattaattga atcaaatgtt taattccttg
    65761 ttgaatatta tcaagcgtaa ccgtacgaaa tgattttttc attaatttaa caaattcatg
    65821 gtctatacgg ttatatactt tctcttcacc gctatatgct ttaattgttt ctattccttt
    65881 aagactttct atgatactag aatttacagc tgcaccggct gccatttctt ccctgttcga
    65941 tttttcatag cttttcacga aagcgaatat tgcaactaca taaaaaggca aagagcatag
    66001 agtgattaaa aacaacgtac tattctgaat agctaacgta accccaacaa gcaacaccat
    66061 accaatatct aaaaatattg ataacgtagc acttgctaaa gcatcaataa tcttactggc
    66121 atcaagaaaa cgagaaataa tttcccctga ttttctcgta gcaaaaaaat tcattggtaa
    66181 agttaacaca tgtttgaaat actgaagcat aattgccata ctcattcgct gtcctaaaat
    66241 aataagaaga taactacgac tatattcaaa caatacccga aataaataga cagcgatcaa
    66301 gccaatggaa attatattta aagttgaacg tgcttgatta gggatgaaaa aatcaagaat
    66361 accttgaaaa taatatgagc ttcctatgcc aaaaaacgta atgaaaatcg atgctaaaac
    66421 gatgtggaat actaatcctt tttgtttcca aattataggc aaaaaagaac ttaatccagc
    66481 tactttttct ttaataggaa tataagtttc tctaggcata ggtaataaaa gaatacctgt
    66541 ccattctttt tgaaacgctt caatcgtttt tctatttttt ccattagcgg gatctgcaat
    66601 ccatagtacg tttcccttcg ttttataaat tacaacgtag tgcatatagc tgttatcaac
    66661 caatatatgt gcaattaatg gtaagataag atctctttct ttccagatct cactatctgc
    66721 ttgaatagcc atacaatcaa aagacaattt ttcaaatgtc tttttcaatc caaatgctga
    66781 agtaccctct aaatcagtcc cagataggtc ccgtaattta tgaatcggga tatcagtttt
    66841 aaaatatttt aaaatcatcg aaaggcaagc aacaccacaa tctttttcat cttgttgctg
    66901 cacaaaagga aatgatttta tcacttatct aacacaactc cttctaaaaa atttttatag
    66961 aaaaattctc aatatagctt taatacagtg aaaatattgt ctcataattc cacaaaaact
    67021 ttgaaaacag ttcttgaaca ctaacttttt cattcctgaa tcaaatttat tttggaaatt
    67081 ttactattca agaataaact tgataagttt caggaagatt tttactgtta atcaaatacg
    67141 tatgttacag tagcaccgta aaaaatatta tttaaagaag cgtgtttaaa catgcaaaat
    67201 gtaaaggaac taagtattgt ggaaatgcac aaactgttgg tggaggcaat aatgcttggg
    67261 gaaaattggg gcaagttgta ggtggactta ccacaggggc agtaggtggt gcaggtttag
    67321 gaacagcaat ttgcggacca gcttgcggtg ttgttggagg gttatatgga gctgtcgctg
    67381 gaggtgctgc agctggctgg gatgctcgaa aaaaataaaa aaggagaata agtagtatgc
    67441 atattaaaaa cacaaaaaca acatttatat tatcttcaga agagttgaaa aatattcaag
    67501 gtgggagtgc cgtgggagtt ttaggcacta ctttttctgg ggcaacagca ggagtaaaac
    67561 tttgttcagc cggaggacct tatgcaatag ctgcttgtgg tgtaggtgga gctttattag
    67621 gtgctggatt cagcatgtgg accggtgcgt aaacaattaa aaaatatcaa acctcaagat
    67681 tggtttgtgt cgattacgaa cataggtatg ttctttatct tttcaaaagt ttggaatgga
    67741 cagaactttt tactaaagct tcttgtgcta gtttttataa ttatattaaa ttatatatgt
    67801 ctttttgact atctttttat atcttcttaa atgtccgcaa agccttattt tatcggctct
    67861 acgggcattt tgcttttgtg gtaaacctca catatctagg tctatcttct tatattttcg
    67921 ctatcaatcg tggttaaaat cgtggtaaat acttctatgc aattagacgc tgaacctctg
    67981 attttgcggt atctatggac gcatgagcgt accagttcat tgtgatacta atgtttgaat
    68041 gtcccatgat atactgtaaa tcttttgggt tcatgttctt gcttgccagc cttgtgcaga
    68101 atgtatggcg tagcgtatgc ggtgtgatat gtggcaaggg gttgtcctta tggtgcttgt
    68161 tgtatttctt taccatacgg acaaataaag tgctgtaatc aatcgcaact tttggcttgc
    68221 ctttctgatt gacaaatagg aagttgctcc gtccgtctat cacaaatggt tttgcctttg
    68281 ggcgtttctt tatcactcgt tgaaatgctt gtatcgtttc cttgcttaat ggcacttgcc
    68341 ttgttccact ctttgtctta ggcgtttcaa tataataacc ttgttcctta ctctttagta
    68401 actggtggtc gataactaca acctcatgga taaaatcaac gtccatgatt gtcagtccgc
    68461 acagttccga gatacgaagt ccagttttca acagtatcag cacatcatca taatacttat
    68521 gatacacgtt atctgtttta atgaatgaca ataaggcttg ttcttgtccc tctgtcaatg
    68581 ccactttctc tttggtatca ttttctagga cttcactcaa cttgaaatca aaagggttct
    68641 tccttacaca atcgtcttgt atggcgatat agaatgacgc ttttaacgag cgtttatggt
    68701 tgttaatggt gttataggaa aagcctttgt ctttcatgcg taacgcccat tctttagcgt
    68761 cagagggttt tatcgtatca atgctcctag cacctaactt gtcctctttc aataaccgca
    68821 tgagttgttc ccgttgtttt attgtgcttt tcttcacatt tgccctctgt gcgttctgtt
    68881 tggcgtagag ttggcaaagc gtcattttct tgcctgtgct gtcgataccg tcctcaatat
    68941 cccgtcttat ctgctgttcc agttctcgaa gtgagggttt ttcccgtttt ccctttggtg
    69001 tcgggtctgt gggtgtcaat ctccaagcgt acacatattt tgtgtttcca aatgcgtcta
    69061 catatttata taagtatttt ccgtctgttc gttggctctc tccagtatgc aggatacgtc
    69121 ctttgctatc ccgtcttttt tctttcatga tgtctgctcc tttccttgtt ggaaagagcc
    69181 ttgatatgac ttgtgttcat cataacacat acaaggctca tttgcattag attgcgtcca
    69241 atttgtcaat gaccttttca aactgtcggc gtttaatctg tatgcggttg ccattcataa
    69301 tgagccagcc agcgtccttg ttttcctctg ccaatcgtct taacttgttt tctccgatac
    69361 ggaaatactt tgacgcttct tcaatggtaa gggtgtattt ttcccataca ggaatatcgt
    69421 tgttattcat cagatacccc ctttcccatg tttcgtgtca tactggatat aggggataag
    69481 gtcggtgcgg tctatcctct gtaagtgggc ggttagttta ctggaaaggg aattgctgta
    69541 tcttgcctta tcaagcatag ttcccacttt ttccagtcca cctaatgcgt catgttcttc
    69601 caagaagtaa aaacttttca cagcggaaat cacgccaccc tctgtgagcc attgctgtgt
    69661 ttcttcaaga gaattatgta gtttcggtac ttgcagtttt aagacttcca cagcccctaa
    69721 aaagcgttcc caaaaccgac acgttttcca tctgctctta ttactttcat ttctgtttgg
    69781 cacgacaaag cgtaggttgt ttgccaatag cccgaaagcc agttccccaa gttccagcgg
    69841 tctgtccttg aatgtcatgg caaaa
//
LOCUS       DACEDD010000009        57941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEDD010000009 DACEDD010000000
VERSION     DACEDD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.009"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWO45_000663"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWO45_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0332162.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWO45_000664"
     CDS             852..1040
                     /locus_tag="IWO45_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0332163.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWO45_000665"
     CDS             1053..1610
                     /locus_tag="IWO45_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0332164.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWO45_000666"
     CDS             1642..1884
                     /locus_tag="IWO45_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0332165.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2225..3439
                     /locus_tag="IWO45_000667"
     CDS             2225..3439
                     /locus_tag="IWO45_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0332166.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3470..4897
                     /locus_tag="IWO45_000668"
     CDS             3470..4897
                     /locus_tag="IWO45_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0332167.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4920..5846
                     /gene="glsA"
                     /locus_tag="IWO45_000669"
     CDS             4920..5846
                     /gene="glsA"
                     /locus_tag="IWO45_000669"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0332168.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6054..7514
                     /locus_tag="IWO45_000670"
     CDS             6054..7514
                     /locus_tag="IWO45_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332169.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7735..9498
                     /locus_tag="IWO45_000671"
     CDS             7735..9498
                     /locus_tag="IWO45_000671"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0332170.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9582..10040
                     /locus_tag="IWO45_000672"
     CDS             9582..10040
                     /locus_tag="IWO45_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0332171.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10149..10832
                     /locus_tag="IWO45_000673"
     CDS             10149..10832
                     /locus_tag="IWO45_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0332172.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10979..11593
                     /locus_tag="IWO45_000674"
     CDS             10979..11593
                     /locus_tag="IWO45_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0332173.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11609..11815
                     /locus_tag="IWO45_000675"
     CDS             11609..11815
                     /locus_tag="IWO45_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332174.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..12911
                     /locus_tag="IWO45_000676"
                     /pseudo
     CDS             12354..12911
                     /locus_tag="IWO45_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12969..15008
                     /locus_tag="IWO45_000677"
     CDS             12969..15008
                     /locus_tag="IWO45_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0332175.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15117..16268)
                     /locus_tag="IWO45_000678"
     CDS             complement(15117..16268)
                     /locus_tag="IWO45_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0332176.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16422..16952)
                     /locus_tag="IWO45_000679"
     CDS             complement(16422..16952)
                     /locus_tag="IWO45_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332177.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17051..17401)
                     /locus_tag="IWO45_000680"
     CDS             complement(17051..17401)
                     /locus_tag="IWO45_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0332178.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="IWO45_000681"
     CDS             complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="IWO45_000681"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0332179.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="IWO45_000682"
     CDS             complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="IWO45_000682"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0332180.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19804..20466)
                     /locus_tag="IWO45_000683"
     CDS             complement(19804..20466)
                     /locus_tag="IWO45_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0332181.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20650..22296
                     /locus_tag="IWO45_000684"
     CDS             20650..22296
                     /locus_tag="IWO45_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0332182.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22287..23369
                     /gene="uxuA"
                     /locus_tag="IWO45_000685"
     CDS             22287..23369
                     /gene="uxuA"
                     /locus_tag="IWO45_000685"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0332183.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23477..25030)
                     /locus_tag="IWO45_000686"
     CDS             complement(23477..25030)
                     /locus_tag="IWO45_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0332184.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            25178..26346
                     /locus_tag="IWO45_000687"
                     /pseudo
     CDS             25178..26346
                     /locus_tag="IWO45_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26472..27551)
                     /locus_tag="IWO45_000688"
     CDS             complement(26472..27551)
                     /locus_tag="IWO45_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0332185.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27654..28094)
                     /locus_tag="IWO45_000689"
     CDS             complement(27654..28094)
                     /locus_tag="IWO45_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0332186.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28151..28561)
                     /locus_tag="IWO45_000690"
     CDS             complement(28151..28561)
                     /locus_tag="IWO45_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0332187.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28741..29175)
                     /locus_tag="IWO45_000691"
     CDS             complement(28741..29175)
                     /locus_tag="IWO45_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0332188.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29275..30123)
                     /locus_tag="IWO45_000692"
     CDS             complement(29275..30123)
                     /locus_tag="IWO45_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0332189.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30512..32278
                     /locus_tag="IWO45_000693"
     CDS             30512..32278
                     /locus_tag="IWO45_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0332190.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32380..33639
                     /locus_tag="IWO45_000694"
     CDS             32380..33639
                     /locus_tag="IWO45_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0332191.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33786..35087
                     /locus_tag="IWO45_000695"
     CDS             33786..35087
                     /locus_tag="IWO45_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0332192.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35090..35941
                     /locus_tag="IWO45_000696"
     CDS             35090..35941
                     /locus_tag="IWO45_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0332193.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35954..36769
                     /locus_tag="IWO45_000697"
     CDS             35954..36769
                     /locus_tag="IWO45_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0332194.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36920..38086
                     /locus_tag="IWO45_000698"
     CDS             36920..38086
                     /locus_tag="IWO45_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0332195.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38316..38963
                     /locus_tag="IWO45_000699"
     CDS             38316..38963
                     /locus_tag="IWO45_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0332196.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39047..39991
                     /locus_tag="IWO45_000700"
     CDS             39047..39991
                     /locus_tag="IWO45_000700"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0332197.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40126..41577
                     /locus_tag="IWO45_000701"
     CDS             40126..41577
                     /locus_tag="IWO45_000701"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0332198.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41598..42434
                     /locus_tag="IWO45_000702"
     CDS             41598..42434
                     /locus_tag="IWO45_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0332199.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42434..43093
                     /locus_tag="IWO45_000703"
     CDS             42434..43093
                     /locus_tag="IWO45_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0332200.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43219..43908
                     /locus_tag="IWO45_000704"
     CDS             43219..43908
                     /locus_tag="IWO45_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332201.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43914..44870
                     /locus_tag="IWO45_000705"
     CDS             43914..44870
                     /locus_tag="IWO45_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0332202.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44914..45426
                     /locus_tag="IWO45_000706"
     CDS             44914..45426
                     /locus_tag="IWO45_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0332203.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45682..46230)
                     /locus_tag="IWO45_000707"
     CDS             complement(45682..46230)
                     /locus_tag="IWO45_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0332204.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46319..47500)
                     /locus_tag="IWO45_000708"
     CDS             complement(46319..47500)
                     /locus_tag="IWO45_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0332205.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47736..49244)
                     /locus_tag="IWO45_000709"
     CDS             complement(47736..49244)
                     /locus_tag="IWO45_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0332206.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49403..49594
                     /locus_tag="IWO45_000710"
     CDS             49403..49594
                     /locus_tag="IWO45_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332207.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49797..50714
                     /locus_tag="IWO45_000711"
     CDS             49797..50714
                     /locus_tag="IWO45_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0332208.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50707..51780
                     /locus_tag="IWO45_000712"
     CDS             50707..51780
                     /locus_tag="IWO45_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332209.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51928..52446
                     /locus_tag="IWO45_000713"
     CDS             51928..52446
                     /locus_tag="IWO45_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332210.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52427..52819
                     /locus_tag="IWO45_000714"
     CDS             52427..52819
                     /locus_tag="IWO45_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0332211.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52895..53830
                     /locus_tag="IWO45_000715"
     CDS             52895..53830
                     /locus_tag="IWO45_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0332212.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53963..54742
                     /locus_tag="IWO45_000716"
     CDS             53963..54742
                     /locus_tag="IWO45_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0332213.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54735..55052
                     /locus_tag="IWO45_000717"
     CDS             54735..55052
                     /locus_tag="IWO45_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0332214.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55296..56906
                     /locus_tag="IWO45_000718"
     CDS             55296..56906
                     /locus_tag="IWO45_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0332215.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57063..57911
                     /locus_tag="IWO45_000719"
     CDS             57063..57911
                     /locus_tag="IWO45_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0332216.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19282 a        10234 c        11311 g        17114 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     2101 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2161 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2221 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2281 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2341 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2401 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2461 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2521 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2581 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2641 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2701 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2761 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2821 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2881 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2941 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     3001 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     3061 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3121 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3181 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3241 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3301 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3361 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3421 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3481 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3541 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3601 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3661 ggcaacggtg aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg
     3721 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     3781 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     3841 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     3901 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     3961 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     4021 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     4081 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     4141 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     4201 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     4261 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     4321 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     4381 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     4441 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     4501 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     4561 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     4621 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     4681 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     4741 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     4801 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     4861 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     4921 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     4981 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     5041 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     5101 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     5161 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     5221 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     5281 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     5341 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     5401 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     5461 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     5521 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     5581 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     5641 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     5701 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     5761 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     5821 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     5881 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     5941 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     6001 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     6061 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     6121 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     6181 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     6241 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     6301 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     6361 cgagtacaga actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa
     6421 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     6481 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     6541 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     6601 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag
     6661 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     6721 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     6781 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     6841 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     6901 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     6961 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     7021 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     7081 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     7141 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     7201 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     7261 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     7321 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     7381 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     7441 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     7501 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     7561 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     7621 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     7681 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     7741 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     7801 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     7861 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     7921 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     7981 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     8041 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     8101 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     8161 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     8221 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     8281 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     8341 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     8401 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
     8461 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
     8521 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
     8581 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
     8641 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
     8701 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
     8761 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
     8821 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
     8881 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
     8941 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
     9001 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
     9061 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
     9121 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
     9181 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
     9241 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
     9301 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
     9361 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
     9421 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
     9481 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
     9541 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
     9601 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
     9661 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
     9721 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
     9781 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
     9841 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
     9901 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
     9961 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    10021 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    10081 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    10141 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    10201 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    10261 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    10321 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    10381 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    10441 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    10501 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    10561 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    10621 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    10681 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    10741 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    10801 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    10861 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    10921 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    10981 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    11041 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    11101 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    11161 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    11221 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    11281 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    11341 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    11401 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    11461 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    11521 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    11581 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    11641 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    11701 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    11761 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    11821 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    11881 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    11941 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    12001 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    12061 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    12121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    12181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    12241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    12301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
    12361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
    12421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
    12481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
    12541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
    12601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
    12661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
    12721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
    12781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
    12841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
    12901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
    12961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
    13021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
    13081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
    13141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
    13201 tatctgaaaa agagatctta gatcaatacc agaatatata cggtgctgct gatgtcaaag
    13261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
    13321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
    13381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
    13441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
    13501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
    13561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
    13621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
    13681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
    13741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
    13801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
    13861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
    13921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
    13981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
    14041 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
    14101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
    14161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
    14221 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
    14281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
    14341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
    14401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
    14461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
    14521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
    14581 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
    14641 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
    14701 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
    14761 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
    14821 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
    14881 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
    14941 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
    15001 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
    15061 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
    15121 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
    15181 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
    15241 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
    15301 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
    15361 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
    15421 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
    15481 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
    15541 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
    15601 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
    15661 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
    15721 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
    15781 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
    15841 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
    15901 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
    15961 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
    16021 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
    16081 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
    16141 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
    16201 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
    16261 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
    16321 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
    16381 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
    16441 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
    16501 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
    16561 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
    16621 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
    16681 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
    16741 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
    16801 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
    16861 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
    16921 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
    16981 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
    17041 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
    17101 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
    17161 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
    17221 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
    17281 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
    17341 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
    17401 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
    17461 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
    17521 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
    17581 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
    17641 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
    17701 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
    17761 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
    17821 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
    17881 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
    17941 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
    18001 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
    18061 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
    18121 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
    18181 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
    18241 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
    18301 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
    18361 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
    18421 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
    18481 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
    18541 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
    18601 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
    18661 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
    18721 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
    18781 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
    18841 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
    18901 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
    18961 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
    19021 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
    19081 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
    19141 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
    19201 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
    19261 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
    19321 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
    19381 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
    19441 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
    19501 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
    19561 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
    19621 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
    19681 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
    19741 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
    19801 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
    19861 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
    19921 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
    19981 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
    20041 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
    20101 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
    20161 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
    20221 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
    20281 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
    20341 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
    20401 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
    20461 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
    20521 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
    20581 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
    20641 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
    20701 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
    20761 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
    20821 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
    20881 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
    20941 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
    21001 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
    21061 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
    21121 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
    21181 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
    21241 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
    21301 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
    21361 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
    21421 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
    21481 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
    21541 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
    21601 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
    21661 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
    21721 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
    21781 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
    21841 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
    21901 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
    21961 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
    22021 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
    22081 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
    22141 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    22201 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    22261 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    22321 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    22381 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    22441 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    22501 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    22561 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    22621 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    22681 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    22741 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    22801 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    22861 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    22921 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    22981 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    23041 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    23101 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    23161 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    23221 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    23281 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    23341 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    23401 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    23461 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    23521 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    23581 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    23641 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    23701 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    23761 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    23821 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    23881 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
    23941 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    24001 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    24061 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
    24121 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    24181 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    24241 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
    24301 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    24361 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    24421 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    24481 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    24541 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
    24601 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    24661 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    24721 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    24781 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    24841 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    24901 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    24961 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
    25021 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    25081 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    25141 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    25201 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    25261 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    25321 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    25381 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
    25441 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
    25501 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    25561 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
    25621 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
    25681 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    25741 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    25801 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
    25861 agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
    25921 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc
    25981 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
    26041 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
    26101 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
    26161 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg tcctgaaacg
    26221 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
    26281 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
    26341 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
    26401 atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
    26461 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
    26521 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
    26581 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
    26641 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
    26701 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
    26761 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
    26821 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtattt
    26881 aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
    26941 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
    27001 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
    27061 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
    27121 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
    27181 tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt
    27241 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
    27301 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
    27361 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
    27421 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
    27481 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
    27541 acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa
    27601 atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc
    27661 tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc
    27721 aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt
    27781 ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc atcacccgtt
    27841 tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta
    27901 ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat
    27961 aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag gaggggagtg
    28021 tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg attaactaaa
    28081 tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac
    28141 acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt
    28201 ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa
    28261 gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat
    28321 aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca
    28381 attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg
    28441 ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat
    28501 tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca
    28561 taaaattacc acctttaatt atattttata caattgaatt ttacacaata taattgtgga
    28621 aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt
    28681 tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc atcataaaca
    28741 ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac gatggacctg
    28801 atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc
    28861 catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt
    28921 gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg
    28981 agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac catacaaggt
    29041 aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc
    29101 aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa aatcgatttc
    29161 cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt
    29221 attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc
    29281 gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa
    29341 ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac
    29401 aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt
    29461 aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact
    29521 catgaccata acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga
    29581 agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa taaacagatt
    29641 aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata
    29701 ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa
    29761 aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga
    29821 agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc ttccgaaaag
    29881 tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt
    29941 caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta atattcctag
    30001 accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc
    30061 taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt
    30121 catttattca cttccttaat tcattaataa tactcttatc ttattataac ttacttttca
    30181 ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta tgtttcattc
    30241 ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag aaaattggag
    30301 aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag actaaaactt
    30361 ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa
    30421 actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac ttatagtgat
    30481 gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc
    30541 ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag
    30601 gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca taacaactga
    30661 aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta
    30721 ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg
    30781 ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt
    30841 gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac
    30901 tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa
    30961 cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg
    31021 agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt acttgacgga
    31081 ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata
    31141 cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa
    31201 cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca
    31261 tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta
    31321 taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc
    31381 agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact
    31441 aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa
    31501 agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg
    31561 gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat
    31621 ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc
    31681 atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt
    31741 cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa
    31801 cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt
    31861 gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg
    31921 gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga
    31981 gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa
    32041 acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga
    32101 agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt actttaatca
    32161 gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca
    32221 gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa
    32281 actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa
    32341 gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa
    32401 cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat
    32461 ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat
    32521 tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg
    32581 aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg
    32641 cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga
    32701 cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac
    32761 aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt
    32821 gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag
    32881 aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt
    32941 ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat
    33001 gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta
    33061 gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac
    33121 ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc
    33181 attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc
    33241 aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg
    33301 gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg
    33361 aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa
    33421 gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat
    33481 gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa
    33541 aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca
    33601 gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt
    33661 tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat
    33721 cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca
    33781 ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac
    33841 ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg
    33901 ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat
    33961 tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca
    34021 ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat cttttagacg
    34081 cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag
    34141 tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc
    34201 tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt
    34261 atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt
    34321 ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg
    34381 tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg
    34441 ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat
    34501 gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag
    34561 gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg
    34621 caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct
    34681 ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag
    34741 acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca
    34801 cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt
    34861 tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc gtaggcggag
    34921 gagctggttc tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc
    34981 cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt
    35041 cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt
    35101 tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt
    35161 aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc
    35221 aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac
    35281 tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt
    35341 cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta
    35401 tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac
    35461 tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta
    35521 ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa
    35581 aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg
    35641 gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca
    35701 agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg
    35761 aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa
    35821 ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt
    35881 attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata
    35941 ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac
    36001 tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt
    36061 tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat
    36121 tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt
    36181 ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac
    36241 ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt
    36301 tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg
    36361 ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt
    36421 ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta
    36481 tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc
    36541 ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag
    36601 tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt
    36661 tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct
    36721 tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc
    36781 gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct
    36841 gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt
    36901 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
    36961 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
    37021 ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
    37081 aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
    37141 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
    37201 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
    37261 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
    37321 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
    37381 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
    37441 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
    37501 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
    37561 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
    37621 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
    37681 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
    37741 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
    37801 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
    37861 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
    37921 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
    37981 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
    38041 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
    38101 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
    38161 tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata
    38221 aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa
    38281 tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta
    38341 gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg
    38401 ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag
    38461 caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata
    38521 tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa
    38581 ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg
    38641 atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga
    38701 atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg
    38761 atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa
    38821 gcggtacggc aacagattgg gatttggata aagatttagg tactacttat tgggaagtca
    38881 aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac
    38941 tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta
    39001 gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac
    39061 gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat
    39121 gactacccag gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag
    39181 ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg
    39241 gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct
    39301 tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat
    39361 gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat
    39421 cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt
    39481 tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat
    39541 caaattgcct ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca
    39601 gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac
    39661 agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta
    39721 tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat
    39781 ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg
    39841 tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg aaattttttc
    39901 tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga
    39961 atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc
    40021 gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt
    40081 tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca
    40141 gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata
    40201 cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc
    40261 gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc
    40321 ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct
    40381 cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg
    40441 ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca
    40501 gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga
    40561 tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt
    40621 gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct
    40681 tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt
    40741 gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt
    40801 atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa
    40861 aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag
    40921 gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca
    40981 gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc
    41041 gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa
    41101 gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct
    41161 acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat
    41221 caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa
    41281 gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta
    41341 gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca
    41401 cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa
    41461 aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa
    41521 atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag
    41581 aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt
    41641 ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg
    41701 tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt
    41761 ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac
    41821 ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt
    41881 caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa
    41941 aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag
    42001 atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca
    42061 atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg
    42121 ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg
    42181 attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg
    42241 agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc
    42301 tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa
    42361 tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt
    42421 cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc
    42481 tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg
    42541 aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt
    42601 atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct
    42661 gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac
    42721 gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc
    42781 aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat
    42841 cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct
    42901 agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa
    42961 gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg cactgattta
    43021 ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatgca
    43081 tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt
    43141 ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata
    43201 tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac
    43261 caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa
    43321 ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc
    43381 tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga
    43441 cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct
    43501 acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct
    43561 ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct
    43621 ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag
    43681 cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag acgttcagaa
    43741 aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt
    43801 caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg
    43861 aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact
    43921 atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag
    43981 gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta
    44041 aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc
    44101 aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata
    44161 gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac
    44221 ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac
    44281 agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc
    44341 tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt
    44401 atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat
    44461 atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta
    44521 gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac
    44581 attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag
    44641 gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa
    44701 tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa
    44761 agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt
    44821 ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg
    44881 taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa
    44941 gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc
    45001 tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa
    45061 agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc
    45121 tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct
    45181 tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa
    45241 ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc
    45301 gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg attcacttat
    45361 caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc
    45421 aattgactat aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat
    45481 catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt
    45541 ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc
    45601 tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct cggcttatcc
    45661 tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc
    45721 ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa
    45781 ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc
    45841 acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac
    45901 taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca
    45961 atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc
    46021 atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga
    46081 aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt
    46141 aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga
    46201 aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt
    46261 gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatatt
    46321 ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa
    46381 ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac
    46441 cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg
    46501 ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt
    46561 catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga
    46621 ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc
    46681 cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca
    46741 tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga
    46801 aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat
    46861 agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga
    46921 tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag
    46981 acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt
    47041 ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt
    47101 ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc ataaataatg
    47161 gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca attggcatca
    47221 atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa
    47281 caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg
    47341 ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca
    47401 caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta
    47461 atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa
    47521 ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta
    47581 cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg
    47641 tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa
    47701 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
    47761 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
    47821 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
    47881 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
    47941 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
    48001 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
    48061 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
    48121 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
    48181 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
    48241 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
    48301 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
    48361 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
    48421 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
    48481 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
    48541 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
    48601 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
    48661 tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc tgggccagga
    48721 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
    48781 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
    48841 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
    48901 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
    48961 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
    49021 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
    49081 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactagcttc
    49141 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
    49201 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
    49261 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
    49321 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
    49381 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
    49441 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
    49501 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
    49561 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
    49621 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
    49681 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
    49741 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
    49801 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aatgcggatc
    49861 aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc
    49921 tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc
    49981 ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg
    50041 aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat
    50101 ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca
    50161 cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt
    50221 tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca
    50281 aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag
    50341 atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct
    50401 tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat
    50461 ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa
    50521 aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag
    50581 aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg
    50641 aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt
    50701 ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat
    50761 catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa
    50821 gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac tccgacgaat
    50881 acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa
    50941 ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta
    51001 gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca
    51061 tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc
    51121 gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat
    51181 tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa
    51241 aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg
    51301 atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg
    51361 ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata
    51421 ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat
    51481 cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg
    51541 acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg
    51601 gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata
    51661 acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct
    51721 gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga
    51781 ttggataagt tggattacca gatgtcattc agataattct gttccaaagg acttgcggca
    51841 gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt
    51901 gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta
    51961 ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca
    52021 atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc
    52081 atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc
    52141 caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca
    52201 gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga
    52261 ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac
    52321 cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt
    52381 tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat
    52441 ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta
    52501 tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac
    52561 gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa aaggcgatga
    52621 tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc
    52681 gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg
    52741 gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt
    52801 caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt
    52861 cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat
    52921 gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg
    52981 ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg
    53041 cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt
    53101 gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct
    53161 tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc
    53221 ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga
    53281 aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc
    53341 cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga
    53401 cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac
    53461 cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc
    53521 cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag
    53581 cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat
    53641 aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg
    53701 taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc
    53761 tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc
    53821 tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt
    53881 ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt
    53941 tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt
    54001 gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt
    54061 gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc
    54121 cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac
    54181 attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg
    54241 ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga
    54301 aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc
    54361 tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt
    54421 tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt
    54481 cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc
    54541 aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc
    54601 aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc
    54661 taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca
    54721 ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg
    54781 tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct
    54841 ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag
    54901 atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga
    54961 ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac
    55021 ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat
    55081 tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga
    55141 tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt
    55201 tattttagga attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat
    55261 gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa
    55321 cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc
    55381 cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg
    55441 ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga
    55501 ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt
    55561 ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt
    55621 tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag
    55681 ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat
    55741 atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg
    55801 tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca
    55861 tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta
    55921 tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt
    55981 ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac
    56041 taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga
    56101 tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat
    56161 tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa
    56221 cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa
    56281 tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga
    56341 ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa
    56401 gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc
    56461 tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag
    56521 caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga
    56581 tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt
    56641 gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc
    56701 gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat
    56761 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
    56821 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
    56881 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
    56941 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
    57001 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
    57061 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
    57121 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
    57181 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
    57241 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
    57301 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
    57361 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
    57421 ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
    57481 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctat gcttatctga
    57541 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
    57601 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
    57661 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
    57721 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
    57781 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
    57841 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
    57901 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa t
//
LOCUS       DACEDD010000010        57375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEDD010000010 DACEDD010000000
VERSION     DACEDD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.010"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..621)
                     /locus_tag="IWO45_000720"
                     /pseudo
     CDS             complement(1..621)
                     /locus_tag="IWO45_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816501.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            1017..1688
                     /locus_tag="IWO45_000721"
     CDS             1017..1688
                     /locus_tag="IWO45_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0332217.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNYPTFDTLEKIMDVLGVTPNELLSGEWKYIDHTEPYIMDIIKREQDFNVSLDYLS
                     ENEFFDDEKECTFYKAYKLIQYIHNYITNEVTELEELMEIKQLIQRQKLERMIKVHKE
                     MRGLDRYREQPKEYKYHDPYDDWIFRQLADIDNRNNIPDTSPQVDFNEADYEDYLKAK
                     WNRGL"
     gene            1735..1986
                     /locus_tag="IWO45_000722"
     CDS             1735..1986
                     /locus_tag="IWO45_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332218.1"
                     /translation="MQSTTNGGKALDRAFSFLLWGNQEEERKSVHFCSINAKGMLIFI
                     VADIWNCYNMDMKKATNLKVLIIFKQPHCMEIRNRLSLK"
     gene            1999..2478
                     /locus_tag="IWO45_000723"
     CDS             1999..2478
                     /locus_tag="IWO45_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated DNA-protein cysteine
                     methyltransferase"
                     /protein_id="HAQ0332219.1"
                     /translation="MANEDKKDFNAMLHDSKDMPKFQIIIDQKSIEKYGGNKMYFAPP
                     IDYDKVMKKVPYGKVITVGKIREHFAKLSGADFTEPITAGIFVSIVAWASYQRSEDET
                     PYWRTLKANGELNAKYPNGIEAQKEKLEAEGHTIIQKGRKNIRYYVKDYENSLFDLK"
     gene            2786..3280
                     /gene="dfrF"
                     /locus_tag="IWO45_000724"
     CDS             2786..3280
                     /gene="dfrF"
                     /locus_tag="IWO45_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0332220.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKNELS
                     RFWI"
     gene            3364..3759
                     /locus_tag="IWO45_000725"
     CDS             3364..3759
                     /locus_tag="IWO45_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011068146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HAQ0332221.1"
                     /translation="MAISNINTIISNDIQKVWNIVLAVDKYNSWRSDLSKTEIINDKQ
                     FIEYTKNGYATTFTVTVAEPYKRWEFDMENSNMKGHWIGVFIDKGNETQIDFTENVIP
                     KKWFMKPFVKTYLKKQQKQFVLDLKKALE"
     gene            3786..4106
                     /locus_tag="IWO45_000726"
     CDS             3786..4106
                     /locus_tag="IWO45_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein TfoX"
                     /protein_id="HAQ0332222.1"
                     /translation="MASSKDYLQFVLEQLSELQEITYRAMMGEFIIYYRGKIVGGIYD
                     DRLLVKPTKSAISYMPTVTYEIPYENAKEMLLVEEVDNKDFLTGLFDVMYDELPTPKP
                     KKKK"
     gene            complement(4915..5667)
                     /locus_tag="IWO45_000727"
     CDS             complement(4915..5667)
                     /locus_tag="IWO45_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0332223.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(5682..6929)
                     /locus_tag="IWO45_000728"
     CDS             complement(5682..6929)
                     /locus_tag="IWO45_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0332224.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(7005..7151)
                     /locus_tag="IWO45_000729"
     CDS             complement(7005..7151)
                     /locus_tag="IWO45_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0332225.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(7255..7566)
                     /locus_tag="IWO45_000730"
     CDS             complement(7255..7566)
                     /locus_tag="IWO45_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0332226.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(7568..7765)
                     /locus_tag="IWO45_000731"
     CDS             complement(7568..7765)
                     /locus_tag="IWO45_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0332227.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(8159..9886)
                     /locus_tag="IWO45_000732"
     CDS             complement(8159..9886)
                     /locus_tag="IWO45_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0332228.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(9879..11072)
                     /locus_tag="IWO45_000733"
     CDS             complement(9879..11072)
                     /locus_tag="IWO45_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0332229.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(11259..11903)
                     /locus_tag="IWO45_000734"
     CDS             complement(11259..11903)
                     /locus_tag="IWO45_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0332230.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(11997..12131)
                     /locus_tag="IWO45_000735"
     CDS             complement(11997..12131)
                     /locus_tag="IWO45_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0332231.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(12133..14265)
                     /gene="feoB"
                     /locus_tag="IWO45_000736"
     CDS             complement(12133..14265)
                     /gene="feoB"
                     /locus_tag="IWO45_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0332232.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(14262..14735)
                     /locus_tag="IWO45_000737"
     CDS             complement(14262..14735)
                     /locus_tag="IWO45_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0332233.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            15136..15360
                     /gene="nrdH"
                     /locus_tag="IWO45_000738"
     CDS             15136..15360
                     /gene="nrdH"
                     /locus_tag="IWO45_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0332234.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            15519..17678
                     /gene="nrdE"
                     /locus_tag="IWO45_000739"
     CDS             15519..17678
                     /gene="nrdE"
                     /locus_tag="IWO45_000739"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0332235.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            17728..18693
                     /gene="nrdF"
                     /locus_tag="IWO45_000740"
     CDS             17728..18693
                     /gene="nrdF"
                     /locus_tag="IWO45_000740"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0332236.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(18782..19921)
                     /locus_tag="IWO45_000741"
     CDS             complement(18782..19921)
                     /locus_tag="IWO45_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0332237.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(20230..20943)
                     /locus_tag="IWO45_000742"
     CDS             complement(20230..20943)
                     /locus_tag="IWO45_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0332238.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(21197..21898)
                     /gene="nagB"
                     /locus_tag="IWO45_000743"
     CDS             complement(21197..21898)
                     /gene="nagB"
                     /locus_tag="IWO45_000743"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0332239.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            22132..23664
                     /locus_tag="IWO45_000744"
     CDS             22132..23664
                     /locus_tag="IWO45_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0332240.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(23855..25057)
                     /locus_tag="IWO45_000745"
     CDS             complement(23855..25057)
                     /locus_tag="IWO45_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0332241.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(25139..25828)
                     /locus_tag="IWO45_000746"
     CDS             complement(25139..25828)
                     /locus_tag="IWO45_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0332242.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(25934..26302)
                     /gene="rplL"
                     /locus_tag="IWO45_000747"
     CDS             complement(25934..26302)
                     /gene="rplL"
                     /locus_tag="IWO45_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0332243.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(26355..26858)
                     /locus_tag="IWO45_000748"
     CDS             complement(26355..26858)
                     /locus_tag="IWO45_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0332244.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(27268..27957)
                     /gene="rplA"
                     /locus_tag="IWO45_000749"
     CDS             complement(27268..27957)
                     /gene="rplA"
                     /locus_tag="IWO45_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0332245.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(28061..28483)
                     /gene="rplK"
                     /locus_tag="IWO45_000750"
     CDS             complement(28061..28483)
                     /gene="rplK"
                     /locus_tag="IWO45_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0332246.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            28826..29494
                     /gene="sdaAB"
                     /locus_tag="IWO45_000751"
     CDS             28826..29494
                     /gene="sdaAB"
                     /locus_tag="IWO45_000751"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0332247.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            29507..30379
                     /gene="sdaAA"
                     /locus_tag="IWO45_000752"
     CDS             29507..30379
                     /gene="sdaAA"
                     /locus_tag="IWO45_000752"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0332248.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(30470..31489)
                     /locus_tag="IWO45_000753"
     CDS             complement(30470..31489)
                     /locus_tag="IWO45_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0332249.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(31662..32549)
                     /locus_tag="IWO45_000754"
     CDS             complement(31662..32549)
                     /locus_tag="IWO45_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0332250.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(32626..33171)
                     /gene="nusG"
                     /locus_tag="IWO45_000755"
     CDS             complement(32626..33171)
                     /gene="nusG"
                     /locus_tag="IWO45_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0332251.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(33272..33442)
                     /gene="secE"
                     /locus_tag="IWO45_000756"
     CDS             complement(33272..33442)
                     /gene="secE"
                     /locus_tag="IWO45_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0332252.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(33466..33618)
                     /gene="rpmG"
                     /locus_tag="IWO45_000757"
     CDS             complement(33466..33618)
                     /gene="rpmG"
                     /locus_tag="IWO45_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0332253.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            33780..34424
                     /locus_tag="IWO45_000758"
     CDS             33780..34424
                     /locus_tag="IWO45_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0332254.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(34480..34983)
                     /locus_tag="IWO45_000759"
     CDS             complement(34480..34983)
                     /locus_tag="IWO45_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0332255.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(34996..35042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            35580..36545
                     /locus_tag="IWO45_000760"
     CDS             35580..36545
                     /locus_tag="IWO45_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0332256.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(36644..37816)
                     /locus_tag="IWO45_000761"
     CDS             complement(36644..37816)
                     /locus_tag="IWO45_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332257.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(37803..38948)
                     /locus_tag="IWO45_000762"
     CDS             complement(37803..38948)
                     /locus_tag="IWO45_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332258.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(38962..39886)
                     /locus_tag="IWO45_000763"
                     /pseudo
     CDS             complement(38962..39886)
                     /locus_tag="IWO45_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            40003..41082
                     /locus_tag="IWO45_000764"
     CDS             40003..41082
                     /locus_tag="IWO45_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0332259.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            41114..41707
                     /locus_tag="IWO45_000765"
     CDS             41114..41707
                     /locus_tag="IWO45_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0332260.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(41817..42044)
                     /locus_tag="IWO45_000766"
     CDS             complement(41817..42044)
                     /locus_tag="IWO45_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0332261.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(42147..44735)
                     /locus_tag="IWO45_000767"
     CDS             complement(42147..44735)
                     /locus_tag="IWO45_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0332262.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(44755..45414)
                     /locus_tag="IWO45_000768"
     CDS             complement(44755..45414)
                     /locus_tag="IWO45_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0332263.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(45463..45966)
                     /gene="trmL"
                     /locus_tag="IWO45_000769"
     CDS             complement(45463..45966)
                     /gene="trmL"
                     /locus_tag="IWO45_000769"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0332264.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(46187..47053)
                     /locus_tag="IWO45_000770"
     CDS             complement(46187..47053)
                     /locus_tag="IWO45_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0332265.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            47162..47803
                     /locus_tag="IWO45_000771"
     CDS             47162..47803
                     /locus_tag="IWO45_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0332266.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(47865..49226)
                     /gene="mgtE"
                     /locus_tag="IWO45_000772"
     CDS             complement(47865..49226)
                     /gene="mgtE"
                     /locus_tag="IWO45_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0332267.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(49244..50143)
                     /locus_tag="IWO45_000773"
     CDS             complement(49244..50143)
                     /locus_tag="IWO45_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0332268.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(50145..50942)
                     /locus_tag="IWO45_000774"
     CDS             complement(50145..50942)
                     /locus_tag="IWO45_000774"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0332269.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(50920..51624)
                     /locus_tag="IWO45_000775"
     CDS             complement(50920..51624)
                     /locus_tag="IWO45_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0332270.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            51755..52330
                     /locus_tag="IWO45_000776"
     CDS             51755..52330
                     /locus_tag="IWO45_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0332271.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            52448..53125
                     /locus_tag="IWO45_000777"
     CDS             52448..53125
                     /locus_tag="IWO45_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0332272.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(53204..55012)
                     /gene="pepF"
                     /locus_tag="IWO45_000778"
     CDS             complement(53204..55012)
                     /gene="pepF"
                     /locus_tag="IWO45_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0332273.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(55188..56186)
                     /locus_tag="IWO45_000779"
     CDS             complement(55188..56186)
                     /locus_tag="IWO45_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0332274.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(56412..57065)
                     /locus_tag="IWO45_000780"
     CDS             complement(56412..57065)
                     /locus_tag="IWO45_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0332275.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        17536 a        11624 c        10010 g        18205 t
ORIGIN      
        1 agcggtagga caatcccgat ttaccagcac cgatataaac agtctttgcg gtgtcgaaat
       61 catcaaactt gctttcgtca aagtggtagc cctcactatt cgagataaat tcctcttttt
      121 cgcatttctt ctttatctgc tctatggtaa agaatggctt ttcgttctta tcatcaatgg
      181 caatatcaag tcttgtgaaa tggaaattat ccagtccgta tcttcgctca cagcgtcgga
      241 acatatctcc aaaggtacta ttcctactgt cgagaatacg gaaaatatca tcacaacctc
      301 tgccagtcat aacaagataa cagcctagcc cttgttggtt gtcctctgtc tttcttgcgt
      361 cccccgatac ataaatatct ccaatctgcc agcgtgcttg ataagtcttg aattttatcg
      421 ttgctggata aacattgaaa atgtcagtcg gtaaatctaa aatgtgcatg attacatctt
      481 cagcggttgc cgtttcaaat acaatgctga tgtaatcaat cttaacggat aaatttttgt
      541 gtgtagttat agtgcatttc tcctttcgta tgccccgttt ttttgcgggg taaaaattgt
      601 gttttttcct ttatttatca cagtttctag gtactatgac atgtatacgc agggcggtgt
      661 tacatatacg ccctttatga acgcctaaag gcgttccctt tcttcctgtg tctgatccgt
      721 tcttaacgct cacgagcctt gtaaggctcg tggctaaacg gaacagcccc agtcagaaac
      781 cttattttcg gtctgatagt ctatgcttat tcatgctatc aaaatgaaaa tacatctttt
      841 ctatgcttac tcaagcgtgt gattgaccct agcgtgctta atcactctat ctgatacgat
      901 aataacgcca ttagaattac ttgtcaagaa gtttttgttg acttttagat atatattaag
      961 tatgatatgc ttataaatac caagaaaagc aatgaaagga gtgtttatac taagtcatgg
     1021 agcattacga aaagaaaatc agtttcttag gagagaacat acagaccata agaaagcata
     1081 gaggaatgaa acaacaggaa cttgcggaca aaatcggtat caatatgcag agcctttcca
     1141 agattgaacg tggcgtgaat tatcctacct ttgatacgct ggaaaagata atggacgtgc
     1201 tgggagtaac acccaatgaa ttattgtcgg gagaatggaa gtatattgac cacaccgagc
     1261 cctatatcat ggatattatc aaacgggaac aagacttcaa tgtttcctta gattacctgt
     1321 ctgaaaatga atttttcgat gatgaaaaag aatgcacatt ttacaaggca tataaactca
     1381 tacagtatat ccataactac attaccaatg aggttacgga gttggaagaa cttatggaaa
     1441 tcaagcagtt gatacagcgt caaaaactgg aacgcatgat aaaagtacat aaggaaatgc
     1501 gagggttaga ccgatacaga gaacagccca aagagtataa ataccatgac ccttatgatg
     1561 attggatatt tcgtcagctt gcggatatag acaacagaaa caacattccc gacacttctc
     1621 cacaagtaga cttcaatgaa gcagactatg aagattatct aaaggcgaaa tggaacagag
     1681 gtctttaatt tcctcttgca tggaagatac agcaaacaaa aagcccagta aagagtgcaa
     1741 agcaccacca atggtggcaa ggctcttgac agggcttttt cttttctgtt gtggggtaat
     1801 caagaggaag aaagaaaaag tgtgcatttt tgttccataa atgctaaggg tatgctgatt
     1861 tttattgtgg cggatatatg gaactgttat aatatggata tgaagaaagc gacaaatttg
     1921 aaagtgttaa taattttcaa acagcctcat tgcatggaaa taagaaacag actatcctta
     1981 aagtaggagg tggcacaaat ggcaaatgaa gataaaaaag attttaatgc tatgttgcat
     2041 gatagtaagg atatgccaaa atttcaaatt atcatagacc agaaaagtat tgagaaatat
     2101 ggtggaaaca aaatgtattt tgctcccccg attgactatg acaaagtaat gaaaaaagtt
     2161 ccgtatggta aagtgattac ggttggaaaa atacgagaac actttgcaaa attgagtggt
     2221 gcagatttta cagagccgat tacagcgggt atatttgttt ctattgtagc gtgggcgagt
     2281 tatcagcgtt ccgaagatga aacaccctat tggagaacat taaaagcaaa cggagaatta
     2341 aatgctaaat atccaaatgg tattgaagca cagaaagaaa aattagaagc agagggacat
     2401 accattattc aaaaggggcg taaaaatata cgatactatg taaaggacta tgaaaattct
     2461 ctttttgatt tgaaataggc aaattctatt tcacaatttc atatctctat acacaaagca
     2521 gttagtctta ggattgactg cttttttcat acctaaattt cagattggag tgatgaaatg
     2581 gtgtttgtta gagctgttca gtaagattat cggtgcataa aattttattg tggtgcagat
     2641 atgaaaatga tataatgtgg atatgaagaa agcgacaaac ttgaatttgt cgagctggtt
     2701 agttcgagat aattttgagt ttgttaagat gtggtgcact tgatggaatc acaggtacga
     2761 catcatatta tggaggatga aatagatgat aggtttgatt gttgcgaggt caaagaataa
     2821 tgttataggc aagaatggta atataccatg gaaaataaag ggagaacaaa agcaatttag
     2881 agagttaaca acgggtaatg tggttattat ggggcgaaag tcttatgaag aaatcggtca
     2941 tccgttgcct aatagaatga atattgttgt ttccaccaca acagagtatc aaggagataa
     3001 tttagtttca gttaaatcat tagaagatgc attattattg gctaaaggac gagatgtata
     3061 catatctggt ggatatggac tatttaagga agctttgcaa atagtagata aaatgtatat
     3121 cacagaagta gatttaaata ttgaagatgg agatacattc tttccagaat ttgatatcaa
     3181 tgattttgaa gttttgatag gggaaacact tggtgaggaa gtgaaatata cgagaacatt
     3241 ttatgtaagg aaaaatgaat tgagtagatt ttggatttag ggtgttttca taaatatatt
     3301 gctttttatg catatataaa ttgttgtaag acaaattcca gtttgaaaag gggaaatgaa
     3361 aaagtggcaa tttcaaatat aaatacaatt attagtaatg atattcaaaa agtttggaac
     3421 attgttttag ctgttgataa atataactca tggcgaagtg atttgagcaa aacagaaata
     3481 ataaatgaca aacaatttat tgaatacaca aaaaatggat atgctactac atttaccgtt
     3541 accgttgcag aaccatataa acgctgggaa tttgatatgg agaatagcaa catgaaaggt
     3601 cattggattg gtgtttttat agacaaaggg aatgaaacac aaatagactt tacagaaaat
     3661 gtgataccta aaaagtggtt tatgaaacct tttgtaaaaa cctatttgaa aaaacaacaa
     3721 aagcaatttg ttctggactt aaagaaagca ttggaataat aaaattcaag attggagggt
     3781 tggaaatggc ttcaagtaaa gattatttac aatttgtttt agaacaatta tcagaattac
     3841 aagaaataac atatcgtgca atgatgggag aatttattat ttattatcgt ggaaaaatcg
     3901 ttggcggtat atatgatgat aggttacttg taaaaccaac aaagtctgca atttcttata
     3961 tgccgacagt tacttatgaa attccatacg aaaacgccaa agaaatgtta ttggtggaag
     4021 aagttgataa taaagatttt ctgacggggt tatttgatgt gatgtatgat gaactaccaa
     4081 cgccaaaacc taaaaagaaa aaataaacag cttctagttt acaatttcat atccatatac
     4141 acaaagcagt tagttttagg attgactgct ttttaaaaag acaccattta ggtgtaatga
     4201 tgtcttttgg gtgttatttt ataggtcata tcaaggctca ttcaatcgtg gtaaattcgt
     4261 ggtaaaatga gttttttcta tacttcaaaa tacttgaaag tatagtgttt atgcggataa
     4321 tcgaaaatta gatataaaat ttattataaa tttttatctt ttattaaagt cagataaaaa
     4381 ttctttggaa atgtaatggt agagatattt ttacaaaagg acttgcagcg ctgtagttgg
     4441 tgctgcagga ggacctattt cggccgttac aggaggtatt ggtaatgtac ttgtcggatg
     4501 tgcattaaag cctgttcctg caagttaaat ttataaaaac aggaaagtaa ttatctcaaa
     4561 tcctatcaag tacaatcata ggattgcact tgacaggaca aattttttaa ctaaaaaatg
     4621 aaaaacactt tctaaaaatt agtagaattt gccaaatctt tcgcatcttc caccatacat
     4681 ttaattttaa ttccccttta tggtacttaa taaaaacaag aaaagaacta ttcatttggc
     4741 ccttttttgg ttccagacca tagaaatatt cctccagtaa ctaatattgc tgttatgcac
     4801 gtaactaata acagctataa aaattggtac aactttaata ggattcagaa aaatttgcac
     4861 tagtgaagga atctatttta ttagaaaaat gcaatctccc taaagaaaaa accactaaac
     4921 agtgctttct ttcaacttct ccttaatttt ttgtgctttc cctaaagtaa agtttctggc
     4981 caactctgaa tcaaaatata gtgaacgttt tttaaaatct atttctttaa cgttaaagat
     5041 atttactaaa cacgctcgac taattcttgt taacatagga tatttctttt caagctcact
     5101 aattcttcca taaaattcgt actgcccatc tttagtacac aatgataatc tatgtggtaa
     5161 ttttgaagct tctacaaaat aaatgtcatt tatatcaaaa gtaaatgttt gagaacctat
     5221 ggaaaaaaca aatgcttgat tcgtaacctg ttttgttgca tctatccttt cttgcgctaa
     5281 taacaataac tcaacaatct cgtttcgata ctcatctagt gtttgatctt ttgttacaaa
     5341 tcctaacgtt tcaactcttc tttttattgt tacgggtaac atctcatcat gagtagttgt
     5401 aaaaatgatt tttgcttgaa catcatattt tctgattact tctgctaatt ctataccgtt
     5461 aacttcatga tttaaatcaa tatctaagaa atatattcct tgctttggtc ggaattgttt
     5521 gaggtatttt ttaacttcta acggactttg tgttttcaat actatcttaa atactttatc
     5581 gtgaaaaaga atgaaattat caataattcg ttctaactga tttaattgta taatttgatc
     5641 ttcacataat attattggat aactcataaa tcctccttcc ctcaaaacaa caaaataact
     5701 tgagtagtaa attgatacgg atcgttttga tagttcacaa acatattggg attcttttta
     5761 ttgatttcct ctatactaga taaacctaac ccttgatgtc ccttttttgt tgttattcct
     5821 tttttcatta gagtatctat agagagtagt atctctctac aactattcga tatcgaaatt
     5881 tctatctgtt tatcatctcg gtaaatagct agagacagtt ctctatcttg actctctata
     5941 gatgcttcaa ttgcattatc gagaataatt ccaagcaccc taatacagtc aaatattgga
     6001 acaggaactt caataatagt acttggacat tcgaatttac aacaaatctt attgttattt
     6061 gcttggtata gcttcgctat caacaaactt ttcaaatatt cattttttat attttttaaa
     6121 taacgatatt caaacttatt ggttagataa gaattagaat agatctctaa ttcttttatt
     6181 tctttttcca atatcgaatc atggttttga ttagcgattt cttttaaact cagcaataaa
     6241 tttttatagt catgtctaaa tctggcaaat ttttcttgat ctttttccag tgaatcagta
     6301 tattttttta gataaaccaa ttcttttctt ttcaattgct gttcatattg ttctcgttgc
     6361 ctaatagtta ttctaataaa taaaaataga acaacaaaca tttgaatgat taaaaatatt
     6421 aagacaccaa acacaaaatg atcaaatact tgatattgat gagcaacata agaaacaaat
     6481 aaaccaattg taagtaaata aatcatgaat atagcagtaa gtttagaact ggactgttca
     6541 acaatcctat ttatagctaa ttttctatat aaccaaataa acatcagcaa aaacaaagaa
     6601 tcaagcatta tctggaatac tacataacca taccctttta tcccttcaaa tgaaaacata
     6661 tacgtcataa ccatggaagt aataatagat ctgaaatcat caataagcat acataatagt
     6721 aatttattta aaagcactat attagttttc ttcttttcct ttaatataaa ataacttccg
     6781 attataggta tagctgcgga aacatcagta aataattcaa caaaaaaagt gagtggtatc
     6841 aataaacata caaaaagata ttttcttttg ttaagatcag tattagtcat ataaaataag
     6901 agaaaaaaga aaaagtttcc cacgaacatt cttaatagat aagcttttgt atcgatagta
     6961 atcaagagat cccctcctga ttttaggata tcataaaaaa aattttagtt acacttttta
     7021 aataatgaaa aaacacattc cactaaattt gatgccggtt ttccttgagg ttttgttcca
     7081 gcacctcctt taattttttt aagctcttga tctgaacatt gtttagcatt tagtctattt
     7141 ttttcttcca ttacgattcc ccctacaaac tataaatact tttatgtcac tgattcaata
     7201 tacctatttt tctaatcaaa aaattttgag acatatttaa tattataagt atcattaaaa
     7261 ttgagattta tctccataat ctgctcgata taaaccattt attccagcag ttcttccaat
     7321 ttcactcaaa tttcttatta attcctcttg atattcagtg aatcttattt tattagtata
     7381 agcagtaaaa tatagataat tcaccaatct atttattaaa ggaccgggat ctaattcata
     7441 tttttgtttt tctaatttta aatatacctt ttgtaaaacc tcaagaatat cagaatattt
     7501 aggatcttta cttatagata tatcattata taattcatga acaatttgct tagcattttt
     7561 tttcatttta gcacttccct ggaattgctc cacctaaaaa accacctata gacattcctg
     7621 caatacaagt agttgccttg gcccaatcga ccgtacattt atttttagtg caatacactc
     7681 catttccata atattttcca ctatgagtgg tacccccata gataagttgt gtctctttaa
     7741 tagacaaaat ttttaaatgt ttcataatct aatcacccct aaattaatgt ttttagacta
     7801 ttattctaac attcattaaa tttcaaaaaa caattccttc ttgaaacgta gcaaaataat
     7861 tcctgaataa gaatttaatc acttttaagt atataaacta tgtatattct ttaatatttt
     7921 ttgagaatgc acttcgtaca taataaactt taatcacatt agtctttcat acctacgatc
     7981 gatctctttg actcgcaccc ataaattcat aatagtttgt cggcgtcttt ggacgtttcg
     8041 aagatgagct acgcattctc tttttttata gacaaaaaag atagagacga acatggagct
     8101 tccaaatttg gatatttgat ccaattttgg aagctccatt tatttcgtgt ctgtcttttt
     8161 attttttcca gctttcgact aattcttggt tctctttgat ccagtctttt gctgcttgct
     8221 gtggatcttt tccattgttg atttccaaca taactttttc catatctttt tctgaccaat
     8281 ggaaattgtc taatactttg taagcttctg gctggtcttc tttcaatcct tttcgaacca
     8341 tcgtatggat ctgctcttcg cttcccattc cgttttcagg atcttctaag tatttcagat
     8401 catatttggc aaacatccaa tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt
     8461 tgatcgcttg acctagtgca acggtcattg ctcctgaaga agatgtctct actttccagt
     8521 ctttcaagtt gtcatatttt tggatcgtgt tttcagcagc cgtaactaca ccagctcctg
     8581 gctcgattcc tatgattttc ttacctgcct gatcggttaa ctcatcgatc gaatttacat
     8641 ccatgtaggc tggcactgct aaaccaactt ttgctcctgt caaattggca cctaggtctt
     8701 cgacttgatc tttatattgt gcatattgtg aaccatgagt tttaggtagc caagctgaaa
     8761 ccatggcatc tgactcgcca tttgaaacag atttccacat gattgaatta tctaatggcg
     8821 tcatcgttac atcatagccc atttgtttca atacttcacc gactacattt gttgaagcaa
     8881 cttctgtatc ccattcaaca taagataagt tgatacgttt tgtttctttg gcagatgaaa
     8941 aactccctat accaatcgca cctaagatca ctactactac agcaccaatg atcagtcctt
     9001 gacgtttttt acttttctgt gttgttgctc ctgcagcttt tttattgttt ggctggttca
     9061 aatgctgtgt aaaacggtca atgatgattg ctaagataac taatgcaacc ccgttgacaa
     9121 atccattccc gacttgtgct ctttgcaatg ctgataatac gccgcgtcct aagccgggtg
     9181 caccgatcat agaggcaata acgaccattg aaagtgacag cattgttgtt tgattgatcc
     9241 ctgccatgat cgtactttta gctaaaggca attctagttt gaatagtttt tgacgacttg
     9301 tgctcccgaa tgaatcagaa gcttctacta attctttagg aacttgtcga atccctaaat
     9361 tcgtaaaacg aacggttggt ggcagagcaa agatgactga ggcaaatacc cctggaacca
     9421 tcccgatacc gaaaaatgca actgctggaa tcaaataaac gaatcctggc attgtctgca
     9481 taaagtcaag gatcggtttg ataatacttt gggctttact gctttttgcc atcaagatcc
     9541 ctaatggtac cccaatgatg atcgagatca cactagataa aagaacaagt gtgacagtat
     9601 tcatcaaatc attccataga ccttggttat aaataaacca tagaccgatt aatgtgaaca
     9661 acgtaagtcc tggacgtttc ttagaaataa agaaagctgc taccgtcaat aacaagataa
     9721 acaaaggagc tggaatcact aacagtaaat ttgtaatccc cgacatcact gcctgtccaa
     9781 tcgtttgtaa aaatgaaaat aggccagaaa aagttgtcgt aaaccattct gtgatatttt
     9841 ctacccagtt tgccactggt aattgatagt tcaatagatt attcatgttc actcacctcg
     9901 cttgtttctg ctaatgcttc aatcacacta cctcggatga cgacaccttt taattttcca
     9961 tcctctacaa ctgcaagtgg agtcggtgag tcataaataa tattgaaaat atcacttacc
    10021 accatgtctt tcccaatcac cgtcacattt tcatctagga agtcgatcaa tggacgtttt
    10081 tgcttcctag cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg tctctttctg
    10141 tcaacagcca tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt
    10201 ccatcagatt cgatattcgt cgtcaatgca ggtaccatga tgttttgagc cgtaagcact
    10261 ttggaacgat cgacttcttc cacaaattca cgtacataat catttgctgg attggtcaat
    10321 atttcttcac ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc
    10381 ccaatccgta aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg
    10441 gcttgcaggt ccagtaattc gtcttgcatt tctcgccgga tcagcggatc aagagccgag
    10501 aacgcttcgt ccatcagcaa gatctcagga tcatttgcta aagctctggc taggccaacc
    10561 cgttgttgca tcccaccaga taactgacta ggatattggt ctttaaatga aagcaaacta
    10621 gaattttcta atgctttttc tgctttttct tgtcgttctt ctttaggtac accacggact
    10681 tctaacccgt attctgtatt ttccaagatc gttcgatgtg gaaacagacc gaagttttgg
    10741 aaaaccatat tgattttatg acgacggact tctcgcaatt cttctttgct taacttcgat
    10801 acatcttctc catcaatata gatactgccc gaagttggct caatcagacg attcaataga
    10861 cggatcaatg tggatttccc actcccggaa agccccatga tcacgaagat ttctccttct
    10921 tttacgtcaa aattcacgtc atacacacca accgttgcac cggttttctt taaaatctcc
    10981 gttttcggct ggtgatcatt catcatatct aaagcggctt gtgtcttttt accaaatatc
    11041 tttgttaaat gttttacttc tacttttacc aaacaaattc tcctttttta tgtaaattca
    11101 tttttctttc tgaaaaaagt gaccacacta tgttaacatc atgtagtcac tttgtcaaaa
    11161 atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg taaataaaag
    11221 gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag ctgatgctcc
    11281 gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa atgaacgatg
    11341 tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc attatgagaa
    11401 attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat tgaacggtcc
    11461 agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt catcttatgt
    11521 attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt cagctcatct
    11581 tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga ttgtacatct
    11641 tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc cccacttcca
    11701 tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg ggtagtctca
    11761 ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc acccacttta
    11821 tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg
    11881 acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt ccctcatctt
    11941 actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat
    12001 cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca attcttaccg
    12061 gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac acttaataag
    12121 aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact ggttcttgga
    12181 ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag caagatacgt
    12241 ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca caaaactaac
    12301 taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct tggcattcat
    12361 ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa
    12421 caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga tttctctgcc
    12481 attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa tattttcttt
    12541 agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac caagcggctg
    12601 aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt cagaaacagg
    12661 ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa caaagataat
    12721 cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta ggaccgctga
    12781 ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat ctccagcaaa
    12841 caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac caagaaaata
    12901 ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta aggcaataat
    12961 cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg
    13021 ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg tagcaatcag
    13081 cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa
    13141 agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa acaatataat
    13201 caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt tcaagatcag
    13261 ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat gttctggtaa
    13321 ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta ctgtttgaat
    13381 agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta accaacgatt
    13441 cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat ttgacacaac
    13501 acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa tcgtctcact
    13561 atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt tttgctgaga
    13621 aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt tcattgcata
    13681 ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta aagctgcttc
    13741 taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg cagactcctg
    13801 aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg gtaaaactgg
    13861 tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt ttttgatgac
    13921 atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact gcgtagttaa
    13981 ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt caggctgttc
    14041 ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca aagaatagat
    14101 gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt cttttttttc
    14161 tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga gattgaacaa
    14221 gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt ttcctccctt
    14281 tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc tcgcactgtc
    14341 agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac atttcgcgtc
    14401 aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc tacgacgatc
    14461 ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga agcagtcttt
    14521 ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg actttttacc
    14581 agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc ggcaacgatc
    14641 ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc gacttgataa
    14701 acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt gtgaaataaa
    14761 tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg caaataaatg
    14821 agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata cttgtgatat
    14881 tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc ttcatagttt
    14941 tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt tttcagctct
    15001 tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat gtagtgtttg
    15061 ttgtttgact tttttactaa gagttttata ctatctatta tagataataa gaatgtgtta
    15121 gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag
    15181 atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa
    15241 cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc tgtcattact
    15301 tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact tgccagctaa
    15361 catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac agcaccagtt
    15421 tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg atttgcaatc
    15481 aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag
    15541 atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt caaatccctt
    15601 tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa ccaaatacga
    15661 tgacttttct atctattatg gaaaaaatca attacttgat cgatcaagaa tatatagaag
    15721 cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa tttttggacg
    15781 agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct caatatgcgt
    15841 tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta gccttcaatg
    15901 cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac gaaatgatcc
    15961 atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg
    16021 aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc gggcgctcaa
    16081 tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact ttatcaaatc
    16141 ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt gtcgttccag
    16201 tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag cgtcaaggag
    16261 ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt tcagcaaaaa
    16321 aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg atcgtacctg
    16381 ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt ccatatagtg
    16441 tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag tatgatcatc
    16501 tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa
    16561 tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat acagcgaacc
    16621 gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag attttacaag
    16681 ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga acggatatct
    16741 catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac tttggtcaat
    16801 ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat atcgatgtcg
    16861 taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt gccatgggct
    16921 tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca ttagacttta
    16981 caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac aatatcgctc
    17041 gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac ggcacttatt
    17101 tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa gcattgtttg
    17161 aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag
    17221 atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt tcatatatca
    17281 acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc caagagaaga
    17341 aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt ccgtattaca
    17401 cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaatg gcacaacaac
    17461 acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca gaaggcattt
    17521 atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta agacattacg
    17581 ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat gatgcagaag
    17641 aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag attcgcttat
    17701 tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc aataaactgg
    17761 aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga gcaattttgg
    17821 ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg
    17881 gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac tgtccaatct
    17941 gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga agccgttttg
    18001 aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat ttttagtaca
    18061 ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa tccttatctg
    18121 caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct agaaaagaaa
    18181 attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac tcctctttat
    18241 tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat catccgagat
    18301 gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa cgagttacct
    18361 gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga gctttacgaa
    18421 aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga agaagtcaag
    18481 acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga tccactcttt
    18541 ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac aggcacaagc
    18601 aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt agaagcgatg
    18661 aaagacgacg attatttgat tggattggaa taaggattta ttttaattca aaaatttttc
    18721 gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact cttttttatg
    18781 cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa acatgttcca
    18841 ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc actgcataga
    18901 caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca atcaatacca
    18961 agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc ttgatcaaat
    19021 ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct tgaaatggag
    19081 agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg acatacggga
    19141 taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga tcgaatattc
    19201 tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta ctgctgatat
    19261 aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta cgaatcgctt
    19321 tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac ataaatagca
    19381 aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa atgatttttg
    19441 agacacttgc agataaactg actaatatga tattgaacaa attactaaga gataaatttg
    19501 ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc gccaattcat
    19561 ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat tgaattgtct
    19621 gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg attacgacca
    19681 aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga aaaacaacga
    19741 ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa actgccgaaa
    19801 gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac acgccgatga
    19861 tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa cgtgattgca
    19921 tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag agttacgcgc
    19981 tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat gagtagacga
    20041 gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag tccctttttc
    20101 caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa cgaaaaatat
    20161 aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta ttttacaacc
    20221 tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt tgtgtacgga
    20281 caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg tatagatatt
    20341 tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt cgattacgat
    20401 atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat ggataaattg
    20461 gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg tgagtgcaat
    20521 agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga tggcgatccc
    20581 taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt
    20641 gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag caatcagcct
    20701 tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat ctcttaatgc
    20761 accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac aaactaaaat
    20821 atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga ttgctccgcc
    20881 aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata ccatatcatt
    20941 catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa aaaaagtgca
    21001 atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc ctttttatga
    21061 ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa aatatgggga
    21121 gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca caacctcttt
    21181 agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc acaacaacat
    21241 tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct tcaatcatcc
    21301 ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct ttacttttca
    21361 taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct ttatcgaaga
    21421 atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc gttccgtcaa
    21481 atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct aagatttgga
    21541 tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct tccagatctt
    21601 ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat aattggacat
    21661 ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac tcatctaagt
    21721 ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta tataaagtaa
    21781 ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata ccttctttga
    21841 tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg atttccatag
    21901 tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt ctttttacaa
    21961 taattagtat agaccaaatt gcattttttt cattttcttg acaataaata ggaatccgtg
    22021 ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc aagcaagtga
    22081 ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat tatgagtata
    22141 ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat
    22201 ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc cggtaaaagt
    22261 acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat ccattggcaa
    22321 aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac attaacaatc
    22381 gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt
    22441 ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc tgggaaactc
    22501 caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc tgagatgagc
    22561 agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct aagtggtgga
    22621 caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt tctgatctta
    22681 gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca gtttcttcag
    22741 gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga tcagatcacc
    22801 acccatatcg cagacatcga gtttggaaag attactaagt atactggcca tctaaaacaa
    22861 gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca tgctcaacaa
    22921 aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc ccgatcaacg
    22981 atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc tccagcttca
    23041 gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt agcagtggaa
    23101 acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa tttgaccatc
    23161 cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttgggaaatc tactttatta
    23221 aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc agataacaca
    23281 aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac accactacaa
    23341 tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca actagctaaa
    23401 gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg tggggaacaa
    23461 actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat tctcgatgaa
    23521 ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat acagaaattt
    23581 ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct tgtgcaacga
    23641 gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga
    23701 caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc tgaccttcgg
    23761 tattagctca aaaaacggca aaatatgagg agctattttt atttggctat tcttattact
    23821 tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg agatttctct
    23881 ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa
    23941 ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat gaaccagccg
    24001 cacctccaat aaagtacata aacataaata cagtattatt tcggttgctt gctgcttcac
    24061 ctagcccttg tactcgtgtt tgattggcga cttgaccaaa ttgattcccg acgtctaata
    24121 aaataatagc taaaagcaaa atggccaaat ggcttccgcc aaccaataaa gcaataaagc
    24181 ttattgtttg cataacaagt cccatataca cgatttttct ttcagaataa gaatctgcca
    24241 gtcgtcccac gataggtgca gcaactgctc cagacaaacc aaaaatcgct aatatccctg
    24301 cttcgaatgt tccccaattg taggcgggac tgctgatata aaaaatcaac gtagaccaaa
    24361 agatcgaaaa tgttccaaac atgaaaaatc cactgactgc cgcttctctt aatagacgtt
    24421 gagaagcaac taaatgcggc aaacttttaa gtgaatcaag gtatgaaaca gctgtactac
    24481 tttgctggac agaacgcggc attttccagt aaagcagaag tgtaagaatt ccgactgcac
    24541 ataaagcaaa taaatagatc atccgccaag aaaaaagact ggcaacaata ccagataccg
    24601 tccgagaaag caaaatcccc acgagtaaac cgctaagcaa tgtacccatg accttccctc
    24661 ttgcttttgg tcccgctagt accgcaccgt aaggaatgat gatttgcggc acgatcgaaa
    24721 gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg
    24781 ccaacagaga gacggcagat aacgctgcca ttcgaatgat caattttgga cgatccacca
    24841 cgtcccctaa aggaactaaa aacagtaacc ctaacgcata gcctaattgg gtcaacatcg
    24901 tcaatatccc gactgcactt gtgctaacag ataaactagc tgctactttt gttccaatcg
    24961 gctggatgta atacatattg gcaacaacaa cgccacaagt gattgataat agtaaaataa
    25021 gcgaagttgt taaaggtttt ttttcattac tcttcattga tttcccccat ttcctatctt
    25081 agtttacaac ttatggaaaa gattggaaat agaaaaatac aactattcgc cacacttatt
    25141 atttgtgctt ttgttttcct tgttttttct tgcgatcttt ataaatgaag acaactaacc
    25201 ctactagcac agtaacactt gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca
    25261 gagcttctcc accacacgca tacaacacag acgaagggat gactccaatc acgatcgaca
    25321 atatgatttg ctgtagaggc aatttcatcg catctgcagc aaaattgacc atagaagatg
    25381 gaataaccgg tatcatatag cctaacatta cacctatttt tggatgtttc atttgtcgga
    25441 ttgcttgcac ccaatgattc gtttcggttt tcttatctaa aaattttaag cgatgcataa
    25501 ggaaaattgc agtgatgtta ccaagagcat tacccaaaat gttgatgatc gcacctgtca
    25561 gtggtccaaa gctcaaacca gcaatgactc caactactga agtaggaata cctggaacag
    25621 cacacatcac cgctgtcaat acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa
    25681 tatgtgcgag tagctctgcc ttactcgctc tcggatttaa aaataattgg atgtctgtct
    25741 gatattctaa atacaaccga tagcccacta ccagtatcag aatgattccc aacgagatca
    25801 atatcaatcg gattattttc tttttcatat ttcctcctga aaaaaatcta tattcattaa
    25861 ataatagttt aacataaaaa caaaaaagca ccgaaatcca acataggatc tcagtgcttt
    25921 taaatagtaa aaattattta acagttacag atgcgccaac ttcttcaagt ttagctttca
    25981 attcttcagc ttcttcttta gatacgcctt ctttaactgg tgcaggagcg ccatcaacta
    26041 cagctttagc ttctttcaag cctaaaccag ttgcttcacg aactgctttg ataactttaa
    26101 ctttttggtc tccagctgca gttaattcta cagtgaattc tgtttgttct tcagcagcag
    26161 cagcgccacc agcagcagct acagctacag gagcagcagc agatacgcca aattcttctt
    26221 caatagcttt aactaggtcg ttaagttcta ggatagttgc ttcttttaac tcagcaacga
    26281 tgttttcaat gttcaatgcc attataattt cctccgttta attgttcttt tttatttaat
    26341 gctttatgcg gcaattaagc tgcatctcca tcttctttgt ctgccactgc tttgacagcg
    26401 taagccacgt tgcggactgg cgcttgtagt acagatagca acatagatag aagtccttcg
    26461 cgatttggca attttgccaa tgctgtgatt tcttccaatg aagagacttt accttcgatg
    26521 ataccgcctt tgatttctaa tgcttttgct tctttagcaa attcgtccat gattttcgct
    26581 ggtgcgacta catcttcgtt actgaatgca acagctgtag gtccagtgaa tacttcgtcc
    26641 atgccttcaa ggccagcttt tttagctgca cgtgaaagaa tagaattttt gataactttc
    26701 atttctacgt tagcatcacg aagttgttta cgtaaacgtg ttacttcatc aactgttaat
    26761 ccgcggtagt caacgactac tactgatgca gctgctgtaa atttttcgct tacttcatca
    26821 acgattgctg ctttttttgc gataattgct tcactcattt ttgagtttca cctcctgaat
    26881 tttgatggaa gagttttttg cactgaaaag gcaagtcact tttatagtta ctttgctcaa
    26941 ccagtattat tcatcaccta ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa
    27001 ggaaactcct ttcgctttgc tcaaccagta ttgttcatca tctcttcact tcgttcatag
    27061 tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg aatatattca
    27121 ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg gtacaagtta
    27181 attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt atttttcagt
    27241 tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat ggataccagg
    27301 tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg ctgatggttt
    27361 agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt catcgtcaaa
    27421 tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt aagttacttt
    27481 accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac cagttttagg
    27541 gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag tagccatcat
    27601 atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt tttgaaccat
    27661 gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt tgtctccttt
    27721 agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa cagcaccacg
    27781 gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt caactgttgc
    27841 atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa caggataaac
    27901 tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt tagccatttt
    27961 ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat atctttcaat
    28021 atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca gtgatcccca
    28081 tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct gcgtttaggt
    28141 cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc gcaactttat
    28201 ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat agaacagcag
    28261 ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa ataacaaccg
    28321 gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg aatcccatga
    28381 tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt gctttacctg
    28441 caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat acctccttaa
    28501 gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat ctgatctgaa
    28561 aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc aaatttattt
    28621 gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg ctaaatttat
    28681 tctaaaaaaa caacaatttt tcgttctcat ttcttctttt atgttacact tgcgctagca
    28741 tggttcaaca gatgaaagca aacaataaca cggtttttta gacccttgta caaagattcc
    28801 tacataacaa aatgaggtgg agaaaatgaa agatttaagg tatagaagtg tctttgatat
    28861 tattggacct gtgatgattg gtccaagtag ctctcacact gccggagctg ctagaatcgg
    28921 gaaaatcata cgtagtatat ttggcgaaca gcctgattct gtagatattt atctatatga
    28981 atcatttgca aaaacctatc gggggcacgg aacagatatt gcattagttg gcggcctgtt
    29041 agggatggaa cccgatgatg aacgtttagc tgattctttg aaattggcat atgaagccgg
    29101 tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa catccaaact ctgtaaagat
    29161 gcttctaaaa aaaggaacac gaaagctttc agtcactggc atttcgatcg gcggtggcaa
    29221 tatccagatt tctgaattga atggattcaa aatttcttta agcatgggta ctcctacctt
    29281 gatcgttgtc caccaagatg ttccaggtat gattgctaaa gtgactagta tactttctga
    29341 gaccaatacg aatatcggca ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat
    29401 tatgataatt gaagtagatg atcctcaagt agaagaaacg gtaaaaagac tcaaacagct
    29461 gccaaatatc gacagcgtaa attatttcga ttaaggagct aaaaaaatgt ttttatcgat
    29521 tgaagaatta gtagatcaag caaaagctta tccaagtgta gcagaattga tgattgcagt
    29581 tgaaatggaa atgagtggcc gttcccgtag tcacatcatc gagatcatgg aaaaaaacct
    29641 ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt gttacctccg tcacaggaat
    29701 taccggtggt gatgcaaaaa aattagatga ttatttaaat gaggcagact ttttaagtgg
    29761 agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat
    29821 ggggttgatt tgtgctacac cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat
    29881 ggctgtccga gatcggcttg atttgacaca tgaacagcaa ttggaattct tatttaccgc
    29941 tggcgctttc gggttagtca tcgcaaacaa cgcttctatc agtggtgcag aagggggatg
    30001 tcaagcagaa gttggttctg caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa
    30061 tgggactcct catcaagcag cacaagcagt agccattaca ttgaaaaata tgatgggact
    30121 gatttgcgat cccgttgcag gcttagttga agtcccgtgt gtcaaacgta atgctttagg
    30181 ctcttctcaa gcttttatct ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc
    30241 gccggatgaa gtcgttgctg caatgtacca agtcggacgt caaatgccgt cgattttcaa
    30301 agaaacagca gaaggtggct tagctgttac gccaactgct aaacgacttg cggaagagat
    30361 tttaaataag agggcgtgat aaagctcctt aatttcgagt attaagagat tagttatctt
    30421 cataacaaaa aacgaagaga ggactaattg cccttctctt cgttttttat taatccatca
    30481 atttcgtatt ttttacttca tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca
    30541 attctttgtt caggtgttca aaaacagatg ttactccttc tgccccacct agattcaacc
    30601 cataaatcac tggtcggcca atcgcaacta agtcagcacc acttgccaga gctttgaaga
    30661 catgctctcc tcgacggaca ccagagtcga aaatcactgg tacacgtttg ttgactactt
    30721 cggcaatttt tggcaatact tcaaaggacg caggacctcc atctagttga cgtccaccat
    30781 gattggatac ccagatacca tctgcccctg cagagatagc gattactgca tcttctggcg
    30841 attgtattcc ttttacgata actggaagat gggtgatttc tttgattgtt ttgatgtcat
    30901 ctggtgtcaa gccttgtttt gcagctgcat aaatttctgc gattcctttt ccttcgccat
    30961 taccacttgc actttgttcg ctatacgcag ctaagttagg cattggtaat ggaaattgga
    31021 attgattgat gacgtcttct tcacggtaac caccgagcgt tgaatctgcc gttaaaatga
    31081 tagctttagc ccctgcttct actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt
    31141 tactcatata taattggaaa aactgcggtg cgcctggcgc tgcatcggct gcgtctttga
    31201 ttgtcgtatt tgcataagtg ctgatggaaa agattgatcc agcagcagct actcctttag
    31261 ctgtatcagc ttcccctttt tcatgtgcca atccttgggc cgctgatggc gcttggataa
    31321 tcggtgtatc taattcaatt ccgaaaacag atgtatgcaa atcggctgag tcgattccgc
    31381 gtaatatccg tggcatgatt ttctttgtat taaaagaagt cgtattttct ttcatcgtcc
    31441 actcatcttc agaaccaccg cgaatatagc caaaagctcc tgcttccatg cgctctttta
    31501 cacgaccttc tagttaggca atgtttacaa tatcgattgg atgttcttca ttacttgtat
    31561 gataagtcat agtagatcct tctttcatct tcttgattac atataaatca tactaaactt
    31621 acaaaaagta agcaaacatt tactctcaga tacttttatc gctattcttt ccaaataaat
    31681 cgacttacaa gctgatctac ttcttggttt tcatgaagca tactatgaga ggcattttca
    31741 cctgtcacta ctttttcctc tatttcattt ccgcgttgct tcaacaacgc atagaccgct
    31801 aatgcactgt ttaacggtac tgttccatca ctcaagtctg tttcatctag ttgaccagca
    31861 atcaagaaaa atcgagtggt tataggaata ttaccgatca tttgctggta atcttgatag
    31921 cggttagaaa cgacagcagg cccctttgct aactcatctg tcagagactg cgcactatca
    31981 tcgacaaaat cattaaaagg cgcgccaatt gctgttaatg tctgaacttg tggcaatgac
    32041 gtatcctgtc catacgttcc taaatagcgt agtgcactaa ctcctcccat ggaatgcccc
    32101 actatgttga ctttatctat gccgtagttt tctttcagat acaaaagaca agttttgatc
    32161 cattctgctt gattccactc gttattttta ttatcttcaa acaatacttg gaccatcgga
    32221 ttgtctgctt ttccagacaa atctcctgtt gcttgtattt gtccatctgt tcctactgtt
    32281 aaaaccaatt cttttactgc ttgctgattt ttttccaaac gccgcaacat ccctttaaag
    32341 gaattttctg tcccgctgta gccgtggaaa aaaagtgttg gaatagtggt tttttctatc
    32401 actttactgg attctgccgt tttttcttta tcagaaactt cagtagtatt tatcttcgtc
    32461 gtctggcaac cacttaacgc aaacaaactg ataaggacta aagctgctgc agttgcacgt
    32521 ttgcataaat ttttgattgg ttctgtcata cacctcttcc tttccatata gaaaaaagaa
    32581 agacttgaaa ggccaatcat atgatcgaca tttcaagtct ctttattaat cgattttatc
    32641 tacttgatcg aaatcaagtt ctgtactggt ttcacgtcca aacatatcga tgttgacttt
    32701 caatttttct ttttctccgt caacttctgt tacttgtcct tctaatccag caaatgcgcc
    32761 ttcgatgatt ttcacgactt cgccaacttc tacttctaga tcattttgtc gcaggctcat
    32821 accgatagaa cgcaagatat gattgacttc ttctggaagt aatggtgctg gtttgcttcc
    32881 ggctccgtgt gaaccaacga atcctgtcac accaggtgtg ttgcggacaa cataccatga
    32941 agcgtctgtc atgatcattt caacaagtac gtatcctggg aatgttttat gaacgatttc
    33001 tttttctttc ccatttttga cttcagtttc tttttcttca ggaacaacga cacggaaaat
    33061 aaagtcggcc attttcatac tttgcgcacg agattcgata tttgctttta ctttgttttc
    33121 atatcctgaa tatgtatgca agacatacca gtttttttca aaagattcca ttgaaggctc
    33181 ctcatttcat tgtaaaataa aaaaacctca gtacacccga agtttttctt tcgcttcatt
    33241 atagcactaa aagtacgttg tggctagcga gttattttaa aatccagcca aataagcttt
    33301 ggatggctgt atccattacg taaaacatca atccgaaaat aatagaagtt tcgatgacta
    33361 ctaacgtatc tttacggagt tgttttctgc ttggccaagt tacttgtttg atttcatctt
    33421 ttacgctttt tagaaatttc atgagacaac ctcccttgct actgtttatt tcgtttcttt
    33481 atgtaatgta tattgattac aatatttaca aaacttattg atttccaaac gttcgccgct
    33541 ttttccttca ctgactgatt ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc
    33601 tgcttttttc tttgccattt acttctgccc ccttgctgag aatcagactt atacttcata
    33661 acagtatcac gtaacttttt acttgtcaat ttgtgaaata aactatctat tgttttggat
    33721 aaaattatga tatgattaat ttgaaattaa gctaaaatga gacaattgga ggtatagcga
    33781 tgctattaaa aacacttgta tataagaaaa aagatctgac aaccgttaca gagactagta
    33841 cattagaaga agcattaaaa attttagaaa cttccggata ccgttgtgtg ccgatcttag
    33901 attcttcagg taatattttc cgtggaaaca tttacaaaat gcatatctat cgtcataaag
    33961 caaatggcgg tgatatgacg cttcctgtca ctcatctttt gaaaaacgct acaaagttca
    34021 tttacctgga tacttctttc ttcaaagtat tctttacgat caaagagctt ccttatattg
    34081 ctgtccttga cagtgaaaat catttttatg gtattttgac acatagtacg ttgcttaata
    34141 tgctctctca atcttggagc gtagaacaag gaagctatgt actgacaatt gcttccacag
    34201 gaaagcaagg agatttggct gctattgcaa aaatcatttc aaaacacagc agcattgcca
    34261 gctgtattac tcttgatatc gaaaaagacg aatacattcg acgtacattg atcacgctac
    34321 cggctaacac tgaagcgaaa gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt
    34381 ataaagtggt agaaattgaa aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa
    34441 gaaaacagct aatctgcttt cttttttcgc tttttataat tagacacgct tttcaaaatc
    34501 aagcgcgcga tctaaataat acatcaatgg tgctgaaaca gccatgacga taaactcact
    34561 taatgcaagc gtgccatagg ttgtccaaaa tgcttggcca cctgttggaa ctaacatata
    34621 agcaatcagt cccatgctga tggtaaagaa gatcgtgttc aatactaaac gcatctttac
    34681 atttttcact ttgttttgca ataaagctgt caggcctaaa gaaagcaatg tttggccgcc
    34741 accaaaaacc acatccatga taccaaaacc aaagaaggca ttatagacga tgaccccgcc
    34801 taaaactccc caaagcaatt tacggttaaa gacaactaaa tgattcaaac tttctgaaat
    34861 acgaaactgg atcggccctg aggatacagg cgcaactaaa atagttaacg ctagataaag
    34921 agccatgatg atgccattta caacgattac ggataaccgt tgtctgcttt tcgttttttg
    34981 cattttctct cctcttttct cctagttttt ttaacgcggg atggttgatg aaccgcggtt
    35041 atgtgaaaca aacaataatt ataacagact tttcatttat ttcaatccgt cttatctgct
    35101 tttaaaaaag caaaagctta aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat
    35161 aaagtaagtg taaggggtca taagaattcc cagtaaagca gacgaagtag gatctgcaag
    35221 aacagaattg ttgtgaaact taaataaata aaaaaggatg tgggaatttg agcaacctct
    35281 tactagagaa cggtctagca agcagctgaa gatctaagtg gacatgccga accttggaac
    35341 ttctccttac gtaagtaatg gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt
    35401 cggacctttt tcattttcct agacttctgc tataataacc aagaattaat tttgtaacag
    35461 atttttttga aaaaacaagt ttctttcatt caaaaaatag aaaaagcttg ctaattattg
    35521 gttaaccagc aaacaggagt attaaaatat cttgttttat tgaatagcag gaggaaatca
    35581 tgaaattcca ttatcatata ttgatcaatc catcggcagg cagcggaaat ggctataaag
    35641 tagcagaaag aattttaccg gtattaaaaa ataagcatat tgactataca cttcattata
    35701 gtgaatacaa aggacacgat gctgagattg ctgaaacttt ggcaaaagaa acattgattc
    35761 cttggataga agaagaacag aaaacaatcg atactttccc ccttttgatc atcgtaggag
    35821 gtgacggcac cttacatcag gtacttgata ctttctatca gatggaagta gaattcccag
    35881 ttgcttatat tccagcaggt tctggagatg attttgctcg aggggcaggc cttccaaaaa
    35941 atcccaaaaa aggacttcag ctgattttag cagctcaatc accagaaaaa gtccacataa
    36001 tggcttatga agaaaaaata agtgaaaaaa aaggaatagc tgttaataat ttcggtatag
    36061 gattagatgc agctatcgtc catgcgacaa atcattcttc cacaaaaaag cgattgaaca
    36121 aatacaactt aggatcattt tcgtatttat tctctattct ccgtgcttta ttcactcaaa
    36181 aaggatttcc aattctggta gatgcaggag gaaaacaatt aagtttttca aacgcatttt
    36241 tatgtacagc aacgaagcat cctttttttg gcggaggaat cgcgattgtt ccaactgctg
    36301 atgcgtctaa accagtcatt gattttgtcg tggtcgaacg aatcaatttc tttaaaatct
    36361 tgtggctcat tcttttactt atacagaaaa aacaattgaa atcgaaatat ttccatcact
    36421 acacaacaaa cagattacgt atcgtttcaa caacaccgca acatggtcaa gaagatggag
    36481 aagaaatgga aaaacgacct tttgatatca caataaccac aactaaacag ttgttttggt
    36541 gttaataaaa gaggttttgt cagaagctac atctggaaca ccgtttattc ggaaaaaagg
    36601 actgtgacac gacttttgtc acagtccttt ttttactatg cagttaattc tttgcccaat
    36661 tgggttgcca tcgatagttt ctttctttac ccaaaataaa ttggatcatt gaaaacaccg
    36721 cagcaaaagc aactaagaca caaccattga accaaaaaga ttctgcaaat gtttgattgt
    36781 aactgagtgt ttcactgatc aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg
    36841 ccgctatttt gctaaccatt tctggcaagt attctgctgg tacaaacacc ccaccgataa
    36901 agcaactgcc tgtgataaaa atatctttga tcccaccgct ggtttctgag ttttttacca
    36961 atgaagtgat acaagccgaa aagctaatga tcggcaataa aaacaagaaa gtatttaaaa
    37021 tatgatacca taccgttata tcccacgagg tgtggatcac taacagaacc attagaagta
    37081 agaagccact gaacaacagc agcgagtaac taatcaaact aaaggttact ttgcgattca
    37141 agtttcgttt ggtaatgggc gcactctggt ttcgtttact gatctctttg cgattaaatg
    37201 ctagattgat gacgctgaaa ccgctaaaaa tggatagaaa caacccataa gacaagaaat
    37261 taaaggttgt gcccgtcaac agctttttca tccgctgact atatcccgca cttagctcaa
    37321 cttctccagt aaccgagagt gccatttgtg tctgttccat agctgcatcg attgttttat
    37381 cttcttcttg aaacagaatc agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg
    37441 ttttagaaaa ggcatctggg cgactctgta tcttgatcgt cggcatcttt cctacttcga
    37501 cttgttcggt aaagtctgca ggcagatgta agataatgtt tacacgatta aaataaagag
    37561 catcatcgat ttctttttgt gaggttccat ccaatgtaac gactgtttcc ttgtcattca
    37621 aatatttacg aaggccatcg actacaggcg actcttcttc actaatcaag gcgattttcg
    37681 catcttgcag agaggtattt tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa
    37741 cagtgataat tacacctatc agtaaattcg atttatttgc tttaagcaat gtccaaatcg
    37801 ttttataaag cgtcataacg cacccctctt tccatccaag catttcctaa cgcaaaaagg
    37861 aaaataaatc cgatcaacca tagaagattg gtataaaaac tagccaaatc agtgtagaaa
    37921 taaagtttat acaaactttc gctgatcaag ttgacgagat ttaattgacc aacaatcgga
    37981 atatgaagat caatccaata cttcacttgt tccgacccca tcataccagc aaaaaagctc
    38041 atcgcaatcg tcactgaaat caagatgcta tccttttgca ccagattcat ttttggtatc
    38101 aaattaccaa ccaatgtacc taacaaaatc gcacacaacg accccagagc ggtaacaatc
    38161 atcaaccatt gccaacgttc accaaagtct acttgataga taaaacgaaa gacagctaac
    38221 ataatataaa tctctgtcaa aacaatcgta aagcttgctg ccaaattagc gattgaaact
    38281 aacgattgct ttctaggaat caaggacaag cgaataccat ttgacgattg atttgcttgc
    38341 tgatcctgta cattgcggac cccccacatc gtcccgtaga gaatcgccat tccaatcaga
    38401 gtgaagaaat aaaaactttt gaaactcata ttacgacctg catgaacttc ttgaaaagat
    38461 acttcttgtt caaaaacagt catgatgggc gcgagatcaa cgttctccac ttctgccact
    38521 ttactcgaaa ttataccaat acgctgtaaa aattgatcca tgacgttttt catcaacgtc
    38581 tgactcattc ctgcttgacc cacatggagc gaaacagatg aaccatcaat gagataaaaa
    38641 ccagccaaat catctgcatt tagcttttca ttcgcttcta cttccgatag ttcctgtact
    38701 tcaaataatg gtttatcatt gacttcgact tgttctaaga ctgtggtaaa attttccgaa
    38761 agcgctgttt gatccgcttt gattacaatt ccagtattaa tcgtttcgag attgccacct
    38821 tgatcaatct cgccaaaggc cgccataaac atcaatccta aaagaattgg aaaggccaat
    38881 gtccaaaaca acatggattt atttcgcagc agtatttttg tgcgatattt ataaaaatgc
    38941 caaaacatta tttcccctcc tctagtctct caaatccttg ccagtaatct ctaaaaagac
    39001 atcattcaag gacgcttctt tggttgctat ttttccgtaa ctgatctttt cttcttgtaa
    39061 gattgtaaga atcggcaaca atactgtatt tagttcagta gttgttactt ggagctgacc
    39121 ttttttataa gcgattccat cagaagctat tgttcgctga caacacgttt tctgggagta
    39181 atgggatgtc gatcgtcaaa cgatccgccg tccgtaccat atcttttaga gattctttgg
    39241 ttcctttagc aatcacctgt ccttgatcca tgatcacgat ctggtcgcaa agaatttcta
    39301 cttcttccat gtaatgtgtt gtatagacaa tggttgctcc ttgtcgattc agctccttga
    39361 tactttctaa aattttattc cgactttggg ggtctacagc tactgtcggc tcatctaaaa
    39421 aaattaaccg cggcttgtgg gcaatcccac atgcgatgtt caaccgacgt ttcaagccac
    39481 cactaagttc tcctggacga aacgaaacat atttttctaa tcccactaat tgaatcacct
    39541 cttcaatcaa ggctttcctt ttctcttttt cggaaacata taaaccgcaa aaataatcga
    39601 tattttcttg cacacttagt tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca
    39661 ttgccttgac agcataattt tcaggagtca ttgttgtccc gaacaattta atggttcctt
    39721 tgtcataatt taacaaagaa agcatacagt tgatggcagt cgatttccca ctgccgtttg
    39781 gtcctaacag acctaagact tctccttctt gtacttgcag atcaaaatga ttcaatgcta
    39841 caaaatctcc atagcgtttg actaatttat caatttcgat aatcatcatg aaaaactcct
    39901 tccaaatttc ttgttgatct gactataata aagtgcaaga aaagaaacta gtgacgattg
    39961 gctaaaccac acatgacatt tgtcacaatg aaggagtctg ctatgcataa atctcggcta
    40021 ctcaacgaaa ctagctttct actggttatc tcattgcttt tgctggtaag tcaaggattc
    40081 gttgctatgc aagttatcta tttattgatc gccgttttat tcactggtgt cagttattta
    40141 ctgcctacaa agccaaattg gattttccaa gttattctat ttcttttatg tgccttttta
    40201 cctgatcttg tcctgtttct gccagcgacg atgcgagtga tcgcccctga agaaagccac
    40261 cttgattact tgtttttgtt aggttttttg ttgatttcga cggttggtaa tcatcccccc
    40321 atcaaggtcg cctttgtcgt aattttgctg tttttttcct tttacttacg aaaaaaagat
    40381 caacgaacga ccgatcttga gaaaaagtac attgtctcca aagatactca ttgggaaaaa
    40441 caacaagagt tatcggaaca aaataaagca ttggccctgt cacaagaaac acaattagcc
    40501 ttgcaaattg tggaagaacg gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat
    40561 ctgctgtcaa gtgcgatttt acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa
    40621 ctaaaagaac cattagaaaa tttaagtgcc accgttcaca ctgggatgga tcggattcgt
    40681 caaagtgtcc atgacctcca tgagacatcg atctcttttc aacactcctt agagtttctg
    40741 atcgctgatt ttccttcttc tgttacgatt gaaggaaact tgtttgaagg attgaatgaa
    40801 acgcagcaaa actgcttctt aatggtcatc aaagaagcct taaccaacag tatcaaacat
    40861 agtcatgcca aaaccgttgt tttacatttt cgaacgttgc cggcctttta tcgcttgcaa
    40921 atcacgaatg atggcaccag cccacaaaaa aattctgctc ccggtattgg tttgaaaacg
    40981 atgcgtcaac gtgttcaaga acaaaaggga caagtccata ttttccaaga aaaccagcaa
    41041 tttcagttaa cgatcatttt accaagggag ggacaccaat gataacagta gtcattattg
    41101 agatgatcca tttgtgacga aatcgttaca aaccatttta gaaagcacga aagagatccg
    41161 tgtgttaggc attggtcatt gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc
    41221 cgatgttttg ctgacggaca tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga
    41281 aattttggct aaatttccca aagccatcct tttgcttttg acgacattca aagatgaaga
    41341 atacatccgc gaagcatttt ccatcggtgt caaagggtat ttaatcaaac agaacttgca
    41401 agcgattatt ccttctgtca aatccgctta taatggacag gccgtatttg gtaatgaaat
    41461 catcgagact ttcactcaaa tgatgaagaa taaaccgacg attgatagtc atgcttatgc
    41521 ttcttttagt gaacgtgagc ttgcaatcat caaagaagta gctgccggga aaaacaataa
    41581 agaaattgca gatgccttgt acttgagtga cggcactgtt cgcaattaca tcagccaatt
    41641 actagaaaag ctagatttac gggatcgcac gcaattggct atctattttt atcagcattt
    41701 gcaataagtc ttatctaaca caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt
    41761 tttttcacat cttctattca attgtcccgc aaaccatttc ctcacgattg aaagaatcaa
    41821 ggattcgcag ggctgccagg attcgttgga ttgttcggcg tgttaggcgg aaaaataggc
    41881 gccttgtcta acgttttttc tagttttgat tcttcacgat cgtctcgttc tacatcagga
    41941 ctttcattca atttgaattc tttttccatc tcatctaact ttttaaagtc attgccagat
    42001 tcttttgcta atttcgctaa tttatcttcg gtttctttag ccataaagta acgcccccct
    42061 tttgatttca gcttagcata tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca
    42121 tttttagcta tttctcatct ttaaaatcaa gcattttttg ccggtataaa atcgtaggga
    42181 gaactgtttt ccataccaat catcggatct actaactggt tatcgatcac ttctgctgta
    42241 agaacgaaat ctttagaaac agcaggttct acctcttctt tcttttcttc tccaacaaat
    42301 gaagaaaaca gatctggttc cactgtttcc attacttggt ttttcgtttc attcgtccca
    42361 ctgtcatctt ctacaggtag cttttctttt acaacccgtg gctcttttgt atagttccca
    42421 tcttcaaatg gatcatttcc gaccaaagaa tcttcataag ctgctagttt ggtccggaga
    42481 gtgatattca ttttatcatt atccatgatc cttgatttca acgtagtcat ccggcttgct
    42541 gattcattcg ctacacaagt gacataggct tgctcttccc ctaacaaaat caacagttcc
    42601 ttactgcgag tcactgccgt gtagagcaaa ttacgctgaa gcatccgctg atattgatgg
    42661 accatgggca aaataaccat cttgaattca ctgccttgtg atttatggat tgagcaacaa
    42721 taagaaagag tgattttatt ccattcgttt cttttataag tgacctcatt tgcatcaaac
    42781 tggataacta attcatccac tttatcttca gaatcttttg ccaagatgat acccgtgatt
    42841 tctcccatat ctccattgaa tacattcaat tcgggagtat tgactaattg gaggactttg
    42901 tctcctatcc gataaacggt gtcgttccaa tgtacttcct tttttccagg tttccctgga
    42961 ttgaatatct cctgcatcat tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt
    43021 ggtgctaata cttggatatc ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt
    43081 ttagagatca agggttcaat ttgataagca tcacttcgga aataggaacg atctttttga
    43141 ttctttgtga aatcttttgg cagacgtcct tctttgatct catgggcaag aggaataata
    43201 ctagaaccat caccttgccg ataaatctga ttgagttcca ttttgggaat ttgttcgatt
    43261 tccaaaagat catggagtac ttgaccagga ccgacagagg gaagctgatc tttgtctcct
    43321 actaagataa cttgcatatt cgtcggaatc gctttgaata atgtgttagc cagccatgta
    43381 tcgaccatag acatctcatc aacaatcaaa agtccgcctt ctaattcttt cgtactgact
    43441 gaaggtgttt tttcccgtcc attcagcccc agaagacgat ggatcgtact ggcaggcaga
    43501 cctgtcgttt cgttcattcg tttagctgct cggccagttg gtgctgcaag tagaatagga
    43561 aaaatttctt gggtatattt tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga
    43621 ttgacgattc cgttgatcac agtagttttc cccgtacctg gtcctcctgt taaaataaat
    43681 agaggggaac ggattgcttc agcgatagct tgttcttgcg aaggtccata ctgtatgccg
    43741 aatatttttt cgatacgccg aatattcttc tgcacttctt tttcaggata atggatttct
    43801 tttttacgac tgagaaggcg ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa
    43861 ctgttttcgt aaagattagt ctcttcttgt tgtatctttc cttcttcaac aagttggata
    43921 attacagtag caacctgatc aggctggatt tcaattgggc gacttgtttc taaaatacgg
    43981 atcgtttgct gaagcaatgt ctccgccggt acgtatgtat ctcctgtttg aactgcatgc
    44041 tgaaaaatct gatgcaagat agctgctcga attcttctcg gtgaatctgc tgcaataccg
    44101 atttgttcgg ctagattatc tgcacgtttg aacccgattc cttctatgtc ttcgactagc
    44161 tggtacggat tttcttcgat gatatccaaa gactcgttct tataggcctg gtagatactg
    44221 aaagctaact gactaccgaa cccgtaacga ttcagtccca tgatcacttg atccatcccg
    44281 tggtttagcc gaatcgtctc agcaagcatt tctctttttg gcttcgtcaa accggagatt
    44341 tgttttaata ccgaaggatc agcaatgatt ttttcgatag catcttctcc cagcaattct
    44401 acaatttttt ctgctgtttt cttcccgatt cctgggaatt tttcgctgga aagatacgct
    44461 acgatcccat ttgcagttgt tggcttttct tgctcatatc tttcgagttt aagctgcctt
    44521 ccgtatttag gatgatccac taattctcca taaaaacgat aagtctcatt ttcttgaata
    44581 tcgccgaaac ttccggtgac tacaatctct ttttcacggt aatcagctgt ggtttcactg
    44641 atgtgtacca aaagcacttt gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact
    44701 gtaccaacga aataaggcat ttcttctggc tgcatcggaa gccgcctcct tcttctatag
    44761 taaaggttct ggtgctgttt cagttcctaa aaaatctgtt tggttccata ctttcagttc
    44821 atatggcaat aatctgcaag gctcttcagt ctctaaaatc gtcaaactac tattgaacaa
    44881 tccacccatt tcacgcaact cacttagctc ttttcctgtc atccattgaa tggctgcagt
    44941 taaagaagct ccatgtccta cgaataaaag tgggccatct ttatgttggg caaccgcatc
    45001 tgctacaaca gtttcgatac gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga
    45061 aggatcatac agatctaaat gattacgcaa attcggcaga ttatccggat atttctggta
    45121 catttcatcg atcgactgac cttctaaagt acccagccct agttctctta gcttatctgt
    45181 ataaaaaatc tctaccggac gagcaagctc tttgttgata ccttctgctg tatcacgtgc
    45241 tcgctttgct gtactggaat aaattgcttt aaaaggaaca tattttacct ttttcccaaa
    45301 tgctttgatt tcttcgtaac tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa
    45361 acgtctttcc tgattccatt gtgtcttccc atgtcgggta aaatatagtt ccatacgcag
    45421 caaactcctg tctttctttt attttactct ataataaact gtctaatcca gcttgtcatt
    45481 ttcatagtga tgcgtcaatt ctaaattagg gaatccttgc tggcgaagtg cttcatacac
    45541 tatcattgca gctgtattcg ataaattcaa tgagcggaca tgctcatcat tcatagggat
    45601 gcgtaaacac ttttcttcgt tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt
    45661 tccaaataaa aagaagtgct cttctccatc agcaaaattt tcgtcactat atactttatg
    45721 ggcaaatttt gagatcaaat gtagtttacg attacctaaa aatgttaaaa aagcttctaa
    45781 attactgtgg taagtaatat ttacatcatt ccaataatcc aaccctgccc gttttagatg
    45841 tttgtcatca gttgaaaaac ctagtggctc gattaaatga agcggagtat tcgtggctgc
    45901 acacgtacgg gcaatatttc cagtgtttgc agggattaat ggttcaaata aaacgatatg
    45961 attcatcagg atcattcctc tcatatttat gttcgtttct attttctgac aatttgattt
    46021 ttgcataaaa aaaaacgtat caactcggtg tcagtcactg cgagtcgtta cgttagtgaa
    46081 gctcttctca ctaactttta tcagaacagg aacctgataa aaaccaatga aaaaacaact
    46141 tgttgctaat ataacaccct caactttttt ttgcaacctc tactcattaa aaactgctag
    46201 aaaaacgttt tctcttagtt ccttttaata attctatgtc aattgtaatc ttcttcaaag
    46261 gattcttata taagtctgct ttgattcgct cttctacctg ccattctaaa ggtgacatgg
    46321 ccaaaagatc tttttgattt tcctctggta tctcaaattc ataggttaca cgctgacgtt
    46381 cagttaattt cattgcttca gcaaacttgg ctactacacg ttcattggaa taactctgtt
    46441 tttcaggttg attcggataa aaagcctgac gtagttcctt taaataattt tcttgtggca
    46501 ccaccttcaa cacttctcca tcttctgaga gtacacgttg gaattcttgg taatgagaag
    46561 gcgaaaagat atttaaaacc gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat
    46621 cagctacaca ccaaaatgct tcggcagatt gttctgtagc tgcgtagact ccttcttttg
    46681 aaatatcaaa tccaacagca ttttgaatct ttttcaattt tgaaagttca ttcaagaagc
    46741 taccttctcc gcagccaaca tctaatagcg agacttcttc aggtaaatgc tgcgcaatag
    46801 tctcaataac gggctgatac atgccagatt ggatcattct acgccttgga acgaacattc
    46861 cttgatcgta ttctgtttta accgcatggg ataaaaaata aagtgttcct tttttggaaa
    46921 ggtcaaaacg gtgattattt ttgcatatca agccattatc agactttttt acttcttctt
    46981 ggcaaaccgg acagcggaac agctgatgat tttctgccaa aaactggcgt cctcggtcaa
    47041 ttttttttaa catagttccc cctcatttct gttttatctt accgttagat gctcactttg
    47101 gcaactgcta aaaatatgat acaatgccta gaagaaagtg aaagataaag gagaagatta
    47161 gatgatcaaa gaattttgcg ctgaaaacta cacatctatt cctctagcaa ttgctagagg
    47221 agccgatcgg atcgaattgt gtgacaactt agcggtcggt ggcacaaccc cgagtactgg
    47281 tgtgatcgaa gaaacgttaa gttatgctgg ggaaaaagat atccccgtaa tgacgattat
    47341 tcgtcctaga gggggcgact tcgtctataa cgatatcgaa ttgaaaatca tgcatactga
    47401 tttgattgaa gcaaagaaat tagggacgga tggcgtagta tttggttgtc ttactccttc
    47461 aggctggcta gacgaagagg ccttagaact attgatcgaa acagcagaag ggttgcagat
    47521 caccttccat atggcttttg acagtattcc tgaagatcgg caattcgaag caatcgattg
    47581 gctggtagaa catggtgttg accgtatctt gactcatggc ggtcctgctg acaaaactat
    47641 tgtagaaaat ttcccacggc ttaaagaatt gattgcctat acagatggcc gcttaatcat
    47701 tttgcctggt ggtggtgtca ctgcagagaa tgcagcacat gtagctaaag aattgaacgt
    47761 aagcgaagta cacggaacca aaatcgtgga tcttcagtta taatcttata aacaaaaaaa
    47821 catttgcaac aggcataatg cttattgcaa atgtttttgt ttttttacat aaaatagccc
    47881 ataaataaag aagcaatatt gaaataaatc agtacactcg tcaaatcgct caaagtcgtg
    47941 ataaagggac cgcttgctac tgctggatca aatcctagac gatccattag catcgggatt
    48001 aaactacctg caagattcgc cactgtgatt gcaaatagca tagccatccc tatgacaaac
    48061 cccaagataa aattatgttt ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca
    48121 gctcctgtga caacgccggt caagacttca gccaaaacaa gtcgaacaaa tccatgatct
    48181 ttctcatcag aaatcgcaag tcgacgaaca gccacagcta gcgactgggt tccagcatta
    48241 ccggcagttc ctgtaatcag cgaaatgaag actgctaaaa tactcgcttc acttaccaag
    48301 tcttcatagt gactgattaa agaagcggtc gacataccta aaaacaacag agtgatcagc
    48361 caaggcaaac gtcgagacgc tgcctttact ggattttcat taatttcttc cacattgacc
    48421 ccggcaagtc ctgaataatc gctagcagct tcatcatcaa taacgtcgat gatatcatcg
    48481 actgtgacga ttcctaacaa atgatcatca taatcggtaa caggaagagc taagaagtca
    48541 taatctctaa atgtttgtgc cacatcttct tggtcgtcgc ctacatgaac tgaaagcact
    48601 cgttcgctca tcagatcaga aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga
    48661 gaaataaccc ctactagctg accttcttga ttaacgacat agatgtaata gattgtttca
    48721 gctacatctg cttcgttctt cagcacgtac atcgcagagc gaacagtttg attggctacg
    48781 atcgaaacaa attcagttgt catgatcgcg ccagccgttt catcctcgta atggagaagt
    48841 tccttgattt cactggcatc gtctgccgac atcaaactta aatatttagc aatttgcttt
    48901 ttatccagcg tattaagcaa gtcaacagca ttatctgtat acatctcaga aagcatatca
    48961 gctgcatagc taggacgcat ttcatctagg tattctttca tatgttcgtt gtcttcttca
    49021 atcacatcga acatatccgc taattcttta ggcgacagat aggaatagat cagctgcctg
    49081 ttctcttctg taagagattg gtagaattgc ccctgctcat agatatgcat ctctaaaaaa
    49141 ctgtttctga atgcctgaat attattttcc cttaaatctt gagtcaactg ggcaaatctt
    49201 tcctctaatt cttgattttc atccaaacca atctcacctc gccttattct tcaaaccgtc
    49261 tcacaactgc ctccatgtct gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa
    49321 atggatggta aaaacgcaga tcataacaat gaagggcttg tctttttatt cccagatcca
    49381 ttcgcccgcc atacatatca tcaccgagca gggcacagcc aattgcggaa aaatgaactc
    49441 ttatttgatg tgtccttcct gtatgcagct ggatatcaac taacgcgatt tctgttagtc
    49501 gcttctctat ccagtactca gtctgtgccg aacgtccttc atcagagacc attcgcttca
    49561 atagcgaaga ataatctcgg gcgatcggca aatcgatatg tccatgtgcc gataaatctg
    49621 aaagccttcc actgactaag gcctgatatt ttttcacaaa cttcttttca cgaagttgca
    49681 tatctaattt tgcgtgcgca aatccatgct ttgcaaaaag catcaaacca gatgtatccc
    49741 gatctagtcg cgtcaccaca tggattactt ggttttcgta accttgtttt ttataataag
    49801 cctttacacg attagccatt gtcccatttg gatggtattg ggcaggaata gacgaaacgc
    49861 ctgctggttt atttactacc agaacgtgtt catcttcata cacgatatcg atcggtgttt
    49921 catccaaaag tacggtttca tgttctcctt cagcaggaat cacaatggtt accttatcat
    49981 ccttagctag agaaaacaag acattttcta cttgatcatt aactttgatt tgaccgcctt
    50041 gaaattttat tttcgctaac agaccctttg agatcccttt ttcttttaaa aaatatttta
    50101 cttgctgaga atgatctttc tcataatccc atgtaaactg catatcaatc ctcactaata
    50161 aaggcatctt tcacacgatg ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt
    50221 tcttctgcta ttcgataata aattgagcgg atatttgctt ttgcgacatt caattgatca
    50281 accgttacaa gataatcttc tgtattttcc aattttattt ctagccattc atcatgggca
    50341 atcacgatcg gagaacccaa agtacgaaac actcgattat tcagtgaagc aatctctgcc
    50401 aactgaaagg cattgatact aggatggatc acagcaccgc cgacactttt attatatgct
    50461 gtcgaaccag taggcgtaga aatcgtcaat ccatctcctc taaaactttc aaacagctcg
    50521 tttttgatgt atacatcggc taccatcgta cgattggccc gtttaatcgt tgattcattc
    50581 agtgctaaaa aatgctggtc cggtgtttca tccaaataac tgatccgaac atccagcaag
    50641 ggataactaa cactttgttc ccggttcgtt cgtaagctgt ctactagttc ttccagctcg
    50701 taatcccgcc aatccgtgta aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca
    50761 tcattcagac aatgactgta gtgatggaac gccgacaata atgtgccgtc tccgccgact
    50821 gagatgacca attctggctc atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg
    50881 ttcaaacgtt ctgtcacttc tatagtcttt gcttctttat tatgaacgat cgctactttc
    50941 atcccctgcc tcctctcttt cgctttcttt cttcaaaagc gttcgatagt agacatcatc
    51001 tgattttcct cttccatggg agaataaccg ctgtgcttct tggatttctt cacggatact
    51061 ggacatctct tcatccaact gataggcagc ttctgccgca cgctgaagtc gttcactcat
    51121 ttcttctgga aaaacgcctt gatatttata attcagcgaa tgctcaatcg ttgcccagaa
    51181 attcatcgca agagtccgaa tctggatttc cgccaaaatt ttcttctcac cgctgatcag
    51241 ctgtacagga tactcgatca ccagatgata agaacggtag ccacttgctt ttttatttgt
    51301 gatgtaatct cgttcttgga gaactttcat gtcttttctt ttacggatga tctcaacaac
    51361 ttgatggata tcttctacaa actgacacat gatccttaac ccggcaatat cagacatttc
    51421 ttcttctaaa cgtcccattg gaatatgccg taattgtgat tttgttacaa tactatcgac
    51481 tggcttgact ctcccagtga caaattcaat cgggacatgt cgattttgtt cacggaactg
    51541 tttgcggatc ccccgcagtt tcactttcaa ttctgataca gtttgttcat atggtgctaa
    51601 aaatgcttcc caatctcgtt ccatagctga tacctctact tcattaacat tcattgttta
    51661 ttctaccata tatgcaaagt tttttcgtac tcttgattta tgcattgacc aaaaaaagaa
    51721 aattaggcaa aatagacaaa aaggagtcga tcaaatgaca caaatgcttg aaatcgaata
    51781 taaatctatg ctgacaaagc aagagtacga gatattcatc gctcactatc aattaacaga
    51841 taaagatttc cgtgtacaga ctaatattta tttcgatacc gttgatgaac agctgaagaa
    51901 acaaaattgt ggcctacgga tccgtttcgt tggtcatcaa gctgaatata cattaaaaac
    51961 acctgctgaa aaagggcgtc tcgaaacaac ggataccttt ataacagaag aagcagaagc
    52021 atttatcttc gaaaaacgtc tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt
    52081 gaatatcgat cctgcttcat taatccaaac cggaaagctt acaacaaaac gggctgaatt
    52141 ccctattgaa gaaggacttt tagctattga tgaaagctgg ggagacaatc tacacgattt
    52201 tgaactggaa ctagaggtag gggatgcatc tgtgggaaaa attgctttta taaatttttt
    52261 aaaacgtttt tctttacctt atcgtcctgc taagaacaag atccaacgta tgcttgaagc
    52321 taaaaattag aaacctttca ttttcacaag gcacttgagt tcattttttt acttttttct
    52381 gttaagttat aggatgtgaa agaatctagt cactagttgt atagaatcta ttttggggga
    52441 gattcatatg atcgaaatct atttatttgt taatccttta ggggaatact gtttcgaaat
    52501 ggaacaacag ttattacaat ttatcgaaga agagtacggg acaccatcaa aagaaaagat
    52561 gcaatttcgc tttcttcctt tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa
    52621 aggaatttct cagaacaatc ttgaggaacg taatcactta ttctcaatga cttattctgc
    52681 agcattggac tgcaaagcgg cgcagtttca aggaaagaaa aaaggccgcc gttttttgat
    52741 caaattgcaa gaagcagtcg gctgtaatga cataccatat tctcaagaac ttgctgaatc
    52801 catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt
    52861 cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca
    52921 cccttctgca gtcgtttaca attatgcctg cgaccgtgat tttggtgttt tagtagaagt
    52981 aaaaggtaca agtagccttt ggagaatcac agagctgtgt aaaacaacag ataactcata
    53041 tcaagttttt aatggtgaac atttacatac aaatgattct cagttccatt caagaactag
    53101 ccatttacac ttgctttcta attaaaaaaa tacgtcgact cccttacaag agagccgacg
    53161 tatttttttc gtttctattc gagaaaatca aaaatagaaa gccttataaa cgatcgatca
    53221 attcttctaa ttcatttaga cgttgttcaa acatcgacat tgcatcttca atatatgcag
    53281 cctgtgtcat atcaactcct gcttttttca ttacttccac aggataatcg ctgttacctg
    53341 cttttaggta agccaagtag ttctctagtg cctctggttc ctggtttagt attttcttcg
    53401 ccagtgctga agcagcagaa aagccagtcg agtattggaa aacatagtaa ttataataaa
    53461 aatgcggtat ccgtgaccat tcgaatttaa tttctgggtc ttcttcgact gctggaccat
    53521 aatattttgc attcaattta ccataactat cacttaggta ttcactagtc aatggcacac
    53581 ctttttcatc ttccgtatgc atgaaatgct cgaattcagc gaattgtgtc tggcggaaaa
    53641 ctgttccttt aaacccatcc aagtaatggt tgagtacata agcccgtaca cgagggtctt
    53701 tttctgtttc caataaatac tccgttagga tattttcatt cgttgtcgaa gcaatttctg
    53761 ctaaaaagat ggagtagtcg ccgtacacat aaggctggtt cgaacgagtg aaataactat
    53821 gaacactatg tcccatttcg tggacaagcg taaatagctg atccagcgta tcatgccaat
    53881 tcaataaaat atatggattt gtgtcatagc ttcccgaaga ataagcatcg cttcgtttcc
    53941 ctttattttc gacaacatcg atccaacgtt cagagaatgc tttttctacg atggccatgt
    54001 attcttcacc cattggtttc agtgcttcta aagctttttc ttttgcttct tcgtacgtaa
    54061 aggtaattgg agcttcccct aagaccggtg tataaagatc atacatgtgc agtttttcca
    54121 cttctaataa acgtttccgt aattccatgt atcgatgtaa caaaggcaaa tgcttgttta
    54181 ccacgtctac caaagtatcg tatacacttt caggaatatg attattgctc aaagacgctt
    54241 ctctggcaga gctgtaatta cggactttcg ctttaaaatt atgtcctttt atatgtgtgc
    54301 ctaaagtaga agcaaatgta tttctaaatt gttcgtaaac actgtacaat cccttaaatg
    54361 ctgcttcgcg cacccttcga tccgtgcttt ctagcaactg accatacacg ccatgagata
    54421 attggactat ttcaccattt tctccttcaa tcgttggaaa aactagatct gcattattca
    54481 aaacagcaaa tgtatcactt gaagcatcaa agatttcacc tgctccagca agaagagatt
    54541 cctgctcagc agataagaca tgggctcgat tatctactat ttgctggata taatggcgat
    54601 agacttccaa ttttggttct tctttgaaat attgccaaat ctggtcatct gacaattgca
    54661 atatttctgg ttcaaaccag gaaacggctt cactaacttt cgaaaacaga ctgcttgctc
    54721 ttgcataaag cgcttggtaa tctgtatttc cagtgtcttg atcgttttta aggtgcgcat
    54781 atacataaat gacttcagtt tggcgataaa ccctcaatac gaattcaatc gcatttaaaa
    54841 attgagaagc gccttgatca agtgttcctt tgtgtttttc agattgtttt aactcttctg
    54901 ataattccaa atatttctca tcaaactctt ggtcgcttga aaagatcttg gtcaagtccc
    54961 aagttaaatt ttcaggcaat tcttctcgtt ttggcaactg ctttacttcc atgaaatcca
    55021 ctccttttct tcccttagct ctatctatca aaatagatca tagtctaaca tccacatgtc
    55081 acagcatcta acttttctac tcattcatgt gtctgcttcc atcaatttta gcataactaa
    55141 atcttgtttt acatagaaaa ccgattccta gcctattttt atacttacta ttttttcaaa
    55201 gaagacaagt gtatgcattc gttaaaaaac tgctgataga ccatctcttc atcaatcata
    55261 ggaaaaagcc attccctttt atgttctttt atttggcaaa gccatgagtt gaaatcagga
    55321 actttctggt tttccaaggc ttcttcgtac aacattcgat aaagaaaaac ctgttcttgt
    55381 aaataaaaga aaaaccgact tttttgatag atccacgatg atagatgcaa aagatgttta
    55441 tgacgcaagt agcattgttc ttgtatggat actgtattct tttgcttgtg aataagttgg
    55501 cgtgtcagcc atttcttccc atcttcactc actttttcta tttttttcat ttggttcatt
    55561 ttaatttcgt tactagtgaa tatttcttta aggtccctac tagaaaaagt ccagcagcta
    55621 ctgcaaaagg aaatatgccc tgatacagtc tctgtaatat gatgatacaa ataaagcttt
    55681 tgtttcttga gatcagcttt ccagcaatgc actcctcttt ttctattata gtagcagcaa
    55741 cttttttcta tcaatgagaa ttgcgattgt ccaagaaaag aattaccaag tatccaccat
    55801 acttggtaac tgttattcaa ataattttgg gttctttcaa taaatctctg gtgagacagt
    55861 ctagaacatt ggatttctat tgctagattc gccaacaaaa tatccggcct ttgccgcaac
    55921 ctcggcaaat aggcttccaa aaatactggt tctactgact gtcccagcca atccatgaag
    55981 cattccttta actgtaagtg ctcttttgat tctctttcac taaagaccga acagttatct
    56041 gatttttgat gggcaaaatg agcacatttt ttctttccct ttttcaaaat cactcttttt
    56101 cgacagccac gacaataata tttctttcct ttttctgcat gtttagcaaa acagcactta
    56161 ttatattcat tcattgcaag cagcattttt ctccctctga acttatttac gtctaagaat
    56221 ttatttacac gaaaaaagag ggcgtgaaaa agctattctg catcaagtaa taagaacagc
    56281 taaacaaaaa tagttcccaa tatttttcgt ttggttgggc ttgttaccgc agatgcaagc
    56341 ttttgaacac atttatacga aaaaaagact gcgacacaat ttttgtcaca gtctcttact
    56401 ataatgctga tttatacaaa ataaaatttc gttcgctcga tagcattacg cgccatcaga
    56461 cattgtccat attcattcaa tgtttctggt gtacgatcgc tcttttcagc aaactccaaa
    56521 attcgagcaa tctgattctc tacatccgct tggttgagat tttccatcca gaagtgaacg
    56581 actaggtaat agcgctcttc gaataaatac aaatcagtcc aagcagactc tagttcgaca
    56641 tctgttgcaa gttggatcac ttgttcaaaa tcttcaaaag caaaagttgc ttgtctttcc
    56701 tgttcttcta gggcgcttcc agataattct tcctccggat cgctgccaat aatctgctga
    56761 cgaagaaagt cactcacatc ttctgaatca acagtagaat cttctgattg agaaatttct
    56821 tcatccggaa gatttttact gataaacagt tctaatccgt ctccttttgg caaaacttgg
    56881 aaagtgacag cttcgcttcc tcggaactcc tcttcgatat caacttcttc caaaatacta
    56941 taaaagaaat tttcgatttc tttgtgattg ccaagtaaat ccaaaaatga aacgccccgt
    57001 tcctctaaat cttcatgacc aattagtaca cggatcgtat tttcattaat atgttccatt
    57061 tccattttgc tacacctcac tttgccacaa tgtttaatat agattcattg tagcgaaatc
    57121 agcttaaatg taaagaaaaa aaatctcgct gttaagcgaa tttaataaaa ccagcggatt
    57181 tttcaacctt tcaagctatc ttatttaact ataacatttt tggcagacga atactatata
    57241 gccgactttt cagctaattt tccctctcta tttttcgtgc aatcatcatc ttctactacc
    57301 tttttataca tctaatcaag aatacttttt gtaaaaacaa aaaaacttgg aaaaactatc
    57361 tttgtaatag tcttt
//
LOCUS       DACEDD010000011        56902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEDD010000011 DACEDD010000000
VERSION     DACEDD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.011"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..978
                     /locus_tag="IWO45_000781"
     CDS             4..978
                     /locus_tag="IWO45_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0332276.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1708..2025
                     /locus_tag="IWO45_000782"
     CDS             1708..2025
                     /locus_tag="IWO45_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332277.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2116..2481)
                     /locus_tag="IWO45_000783"
     CDS             complement(2116..2481)
                     /locus_tag="IWO45_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332278.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2689..3171
                     /locus_tag="IWO45_000784"
     CDS             2689..3171
                     /locus_tag="IWO45_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332279.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3368..4258
                     /gene="pstS"
                     /locus_tag="IWO45_000785"
     CDS             3368..4258
                     /gene="pstS"
                     /locus_tag="IWO45_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0332280.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(4545..5819)
                     /locus_tag="IWO45_000786"
     CDS             complement(4545..5819)
                     /locus_tag="IWO45_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0332281.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            6092..8563
                     /locus_tag="IWO45_000787"
     CDS             6092..8563
                     /locus_tag="IWO45_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0332282.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HANQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(8666..10402)
                     /locus_tag="IWO45_000788"
     CDS             complement(8666..10402)
                     /locus_tag="IWO45_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0332283.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(10399..11103)
                     /locus_tag="IWO45_000789"
     CDS             complement(10399..11103)
                     /locus_tag="IWO45_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0332284.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            11234..11737
                     /locus_tag="IWO45_000790"
     CDS             11234..11737
                     /locus_tag="IWO45_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0332285.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            11697..12035
                     /locus_tag="IWO45_000791"
     CDS             11697..12035
                     /locus_tag="IWO45_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0332286.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            12056..13474
                     /gene="ffh"
                     /locus_tag="IWO45_000792"
     CDS             12056..13474
                     /gene="ffh"
                     /locus_tag="IWO45_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0332287.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            13586..14164
                     /locus_tag="IWO45_000793"
     CDS             13586..14164
                     /locus_tag="IWO45_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0332288.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            14168..14689
                     /locus_tag="IWO45_000794"
     CDS             14168..14689
                     /locus_tag="IWO45_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0332289.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(14768..15322)
                     /locus_tag="IWO45_000795"
     CDS             complement(14768..15322)
                     /locus_tag="IWO45_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0332290.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            15575..15850
                     /gene="rpsP"
                     /locus_tag="IWO45_000796"
     CDS             15575..15850
                     /gene="rpsP"
                     /locus_tag="IWO45_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0332291.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15862..16113
                     /locus_tag="IWO45_000797"
     CDS             15862..16113
                     /locus_tag="IWO45_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0332292.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            16238..17029
                     /locus_tag="IWO45_000798"
     CDS             16238..17029
                     /locus_tag="IWO45_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332293.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            17199..17720
                     /gene="rimM"
                     /locus_tag="IWO45_000799"
     CDS             17199..17720
                     /gene="rimM"
                     /locus_tag="IWO45_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0332294.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            17710..18471
                     /gene="trmD"
                     /locus_tag="IWO45_000800"
     CDS             17710..18471
                     /gene="trmD"
                     /locus_tag="IWO45_000800"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0332295.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            18607..18954
                     /gene="rplS"
                     /locus_tag="IWO45_000801"
     CDS             18607..18954
                     /gene="rplS"
                     /locus_tag="IWO45_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0332296.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(19086..20222)
                     /locus_tag="IWO45_000802"
     CDS             complement(19086..20222)
                     /locus_tag="IWO45_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0332297.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(20338..20904)
                     /locus_tag="IWO45_000803"
     CDS             complement(20338..20904)
                     /locus_tag="IWO45_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332298.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(21040..21693)
                     /locus_tag="IWO45_000804"
     CDS             complement(21040..21693)
                     /locus_tag="IWO45_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332299.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(21743..22171)
                     /locus_tag="IWO45_000805"
     CDS             complement(21743..22171)
                     /locus_tag="IWO45_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0332300.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(22176..22550)
                     /locus_tag="IWO45_000806"
     CDS             complement(22176..22550)
                     /locus_tag="IWO45_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0332301.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            22854..23039
                     /locus_tag="IWO45_000807"
     CDS             22854..23039
                     /locus_tag="IWO45_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0332302.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            23052..23300
                     /locus_tag="IWO45_000808"
     CDS             23052..23300
                     /locus_tag="IWO45_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332303.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(23603..24061)
                     /locus_tag="IWO45_000809"
     CDS             complement(23603..24061)
                     /locus_tag="IWO45_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332304.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            24229..24546
                     /locus_tag="IWO45_000810"
     CDS             24229..24546
                     /locus_tag="IWO45_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332305.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            24479..24958
                     /locus_tag="IWO45_000811"
     CDS             24479..24958
                     /locus_tag="IWO45_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAQ0332306.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            25408..25839
                     /locus_tag="IWO45_000812"
     CDS             25408..25839
                     /locus_tag="IWO45_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332307.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            25836..26162
                     /locus_tag="IWO45_000813"
     CDS             25836..26162
                     /locus_tag="IWO45_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0332308.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            26281..26967
                     /locus_tag="IWO45_000814"
     CDS             26281..26967
                     /locus_tag="IWO45_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0332309.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            26942..28291
                     /locus_tag="IWO45_000815"
     CDS             26942..28291
                     /locus_tag="IWO45_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0332310.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            28282..28755
                     /locus_tag="IWO45_000816"
     CDS             28282..28755
                     /locus_tag="IWO45_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0332311.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            28757..29257
                     /locus_tag="IWO45_000817"
     CDS             28757..29257
                     /locus_tag="IWO45_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAQ0332312.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            29272..31584
                     /locus_tag="IWO45_000818"
     CDS             29272..31584
                     /locus_tag="IWO45_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0332313.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            31831..32148
                     /locus_tag="IWO45_000819"
     CDS             31831..32148
                     /locus_tag="IWO45_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAQ0332314.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            32145..32306
                     /locus_tag="IWO45_000820"
     CDS             32145..32306
                     /locus_tag="IWO45_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0332315.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            32303..32608
                     /locus_tag="IWO45_000821"
     CDS             32303..32608
                     /locus_tag="IWO45_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332316.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTK
                     RRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            32608..32922
                     /locus_tag="IWO45_000822"
     CDS             32608..32922
                     /locus_tag="IWO45_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332317.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            32915..33445
                     /locus_tag="IWO45_000823"
     CDS             32915..33445
                     /locus_tag="IWO45_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0332318.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            33442..33711
                     /locus_tag="IWO45_000824"
     CDS             33442..33711
                     /locus_tag="IWO45_000824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332319.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            33841..34128
                     /locus_tag="IWO45_000825"
     CDS             33841..34128
                     /locus_tag="IWO45_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332320.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            34615..35028
                     /locus_tag="IWO45_000826"
     CDS             34615..35028
                     /locus_tag="IWO45_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0332321.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            35809..36039
                     /locus_tag="IWO45_000827"
     CDS             35809..36039
                     /locus_tag="IWO45_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332322.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            36224..36769
                     /locus_tag="IWO45_000828"
     CDS             36224..36769
                     /locus_tag="IWO45_000828"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0332323.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            36770..37087
                     /locus_tag="IWO45_000829"
     CDS             36770..37087
                     /locus_tag="IWO45_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0332324.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(37244..37408)
                     /locus_tag="IWO45_000830"
     CDS             complement(37244..37408)
                     /locus_tag="IWO45_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAQ0332325.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            37510..38031
                     /locus_tag="IWO45_000831"
     CDS             37510..38031
                     /locus_tag="IWO45_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ0332326.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            38003..38458
                     /locus_tag="IWO45_000832"
                     /pseudo
     CDS             38003..38458
                     /locus_tag="IWO45_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(38498..38656)
                     /locus_tag="IWO45_000833"
     CDS             complement(38498..38656)
                     /locus_tag="IWO45_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HAQ0332327.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            38749..39510
                     /locus_tag="IWO45_000834"
     CDS             38749..39510
                     /locus_tag="IWO45_000834"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0332328.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            39697..41049
                     /locus_tag="IWO45_000835"
                     /pseudo
     CDS             39697..41049
                     /locus_tag="IWO45_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            41065..42285
                     /locus_tag="IWO45_000836"
     CDS             41065..42285
                     /locus_tag="IWO45_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0332329.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            42320..42997
                     /locus_tag="IWO45_000837"
     CDS             42320..42997
                     /locus_tag="IWO45_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0332330.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            42994..44187
                     /locus_tag="IWO45_000838"
     CDS             42994..44187
                     /locus_tag="IWO45_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0332331.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            44202..44528
                     /locus_tag="IWO45_000839"
     CDS             44202..44528
                     /locus_tag="IWO45_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0332332.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            44532..44867
                     /locus_tag="IWO45_000840"
     CDS             44532..44867
                     /locus_tag="IWO45_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0332333.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            44864..45259
                     /locus_tag="IWO45_000841"
     CDS             44864..45259
                     /locus_tag="IWO45_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332334.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            45256..45627
                     /locus_tag="IWO45_000842"
     CDS             45256..45627
                     /locus_tag="IWO45_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332335.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            45643..46203
                     /locus_tag="IWO45_000843"
     CDS             45643..46203
                     /locus_tag="IWO45_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0332336.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            46328..46639
                     /locus_tag="IWO45_000844"
     CDS             46328..46639
                     /locus_tag="IWO45_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332337.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            46663..46854
                     /locus_tag="IWO45_000845"
     CDS             46663..46854
                     /locus_tag="IWO45_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332338.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            46854..50710
                     /locus_tag="IWO45_000846"
                     /pseudo
     CDS             46854..50710
                     /locus_tag="IWO45_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            50711..51583
                     /locus_tag="IWO45_000847"
     CDS             50711..51583
                     /locus_tag="IWO45_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332339.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            51580..52629
                     /locus_tag="IWO45_000848"
     CDS             51580..52629
                     /locus_tag="IWO45_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332340.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            52633..53457
                     /locus_tag="IWO45_000849"
     CDS             52633..53457
                     /locus_tag="IWO45_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332341.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            53475..55535
                     /locus_tag="IWO45_000850"
     CDS             53475..55535
                     /locus_tag="IWO45_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ0332342.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            55698..56144
                     /locus_tag="IWO45_000851"
     CDS             55698..56144
                     /locus_tag="IWO45_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332343.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            56146..56283
                     /locus_tag="IWO45_000852"
     CDS             56146..56283
                     /locus_tag="IWO45_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0332344.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            56318..56560
                     /locus_tag="IWO45_000853"
     CDS             56318..56560
                     /locus_tag="IWO45_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0332345.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            56576..56773
                     /locus_tag="IWO45_000854"
     CDS             56576..56773
                     /locus_tag="IWO45_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0332346.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            56784..>56902
                     /locus_tag="IWO45_000855"
     CDS             56784..>56902
                     /locus_tag="IWO45_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0332347.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
BASE COUNT        19317 a         9692 c        11985 g        15908 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcctgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactgt gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat tttttcggta
     1681 tttaatattt aattaggagg taatattttg cttttttata gtaattttat cttaatagta
     1741 gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaatgc aagtataggt
     1801 tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca aactattttt
     1861 tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt gaatattata
     1921 gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc aattattaca
     1981 caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag
     2041 ttttggtgat ataagaaaaa catatatgta taaaaagaag aaagtagctt ctctttgtac
     2101 atatatggtt tgtttttatg gatgccgagc ttttttataa gataaggcta ccgcccataa
     2161 cgacccaagg atttgtggaa ctaagtaaat acctgcaaga agcaagatca gctgggtcgg
     2221 ttttaaagga tgatagaagt aacgccattc agtaaaattg gcgcaaaata aataggaagt
     2281 gacagtagat ataatattgg caatgaataa agatataata cgcctgttcc tttgaaaata
     2341 atatccagca aaaagagata aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg
     2401 aaaatcaagc agtaaggcga aatgcacata aggaacaaaa gataaaagca acggaaggtt
     2461 tttccagcaa gtttttatca tctttcatca ccacctaaca agtgttatta ttttgttagt
     2521 tttattgtaa attgaatcct gcaataatca attacttttt cattatttct agattaaatg
     2581 aataaactct catgccatta tctctaaaaa aaaagttttt ccccctaatt tttttagata
     2641 gaaaattgta tagtatgatg agagaaaaag tagtttgggg gaacgttaat gagaagaaaa
     2701 gcagtaatcg atcggcttta tttatttttt tggttttttc ctatcgtttt gctacctttt
     2761 acatgggtaa atgctgttgt acaaggtgcg gagatattag gcctgatcca aggaagcgga
     2821 tttgtggttt ctaggaattt tccagctttg attgttttct atttagtgat aagtgtaggt
     2881 ctcttattac caaaatctct gatcaatcac tacaattggc agctgcttta cttttcttgc
     2941 ttattagtat ttacatctat gttttctttg ctgcctcgct tgattatcgg ccctaatggt
     3001 ttgagcgaga gtggattgaa ggatttagct tattcctcca tgtatttctt gacgatcaaa
     3061 ccatggttct atttagtaat tgcctcattt attcttgcaa cattttttta cttctggaca
     3121 gaaatcagga taaaaagtga acgatctgta tctataaaaa ataactattg atggtggatc
     3181 atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt ctcatatgct ttttttatta
     3241 ttatgtaaag aatgcgaaaa acgtttacat gatttttaca taacttaact atatttttac
     3301 agcaaccata cgagggattt acagtcgact gataaacttt agttgtaaat aagaaaggag
     3361 caataaaatg aaaaaagtat tgtttactct aggtatgatg ggaattcttt tagccggatg
     3421 cggctcagga tcaggaaata cagatggctc tgccacacaa aataattcaa gtgactcaaa
     3481 tgaacaagta aaaatcgttg cggtaggttc gacagcatta cagccgttag tcgatgcagc
     3541 acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag
     3601 cggaactggt ttgagccaag tcgaagctgg agcagttact atcggaaact ctgatgtatt
     3661 tgcagaagaa agagacggag ttgatgcttc aaaattagtg gatcataaag tagcagtggt
     3721 cgggatggca ccaatcgtca ataaagatac agatgtcaaa gatatcacca aacaggaatt
     3781 gattgatatc tttacaggaa aaatcactaa ctggaaggaa gttggcggga aagatcaaaa
     3841 aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc gcgacattcg aaaaatgggg
     3901 actggatggt gctacccctg tgcagtccca agaacaagat tcatcaggaa cagttcggca
     3961 gctggtaagt caaacaccag gagcaatcag ctatttagct ttctcatatc tcgatgattc
     4021 cacacaagca ctaagtatcg acggtgtaga accaaaagaa gagaatgtag cagataacag
     4081 ctggggaatc tggtcatatg aacatatgta cacgaacgga aaaccttccc ctgaagtcca
     4141 aaaattctta gactacatga tgacagaaga aatccaagaa ggacctgtca aagaattggg
     4201 ctatttacca atcacaatga tggaagtaga acgtgatcac gaaggaaata tcaaataact
     4261 agcagataaa catgccacgg tagtcgttac tattaaggaa aaatcaacta tgtttcccca
     4321 catatacatt tttcctttaa ttgtgaaaaa tagttcctca tattttgccg ttttttgagc
     4381 taataccgag ggtcagctga ttgaacaccg tttattcgaa aaaaagagct acgacataga
     4441 actgtcgtaa ctttttttgg ctcttcgtca aatcgtgttg gtgaagacaa atttgaataa
     4501 aacttagtcc gatatgcggc gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc
     4561 agaccttgtt gtaaatagat ccgtgcccga aagttataaa agttgcgata accaaacgca
     4621 attcgtttaa tcagcttgat cttgttattc agcccctcag tgacgccgtt tgagtgggac
     4681 accttaaagg catttgaaat acccgattgg tagcaatgga agatcgtgaa tttcttttta
     4741 aacgactgag gtaacgtaga tgaaattgtt ttaagggttt cttgaaagcc ttccgtacct
     4801 cgtttaacga aataatagcg taataattga caaaaatgat acgcagttcg caattcttcg
     4861 ttgtagctta acagctcatc aacgatttct gtttgactaa ttggtcgttt aaacagtcga
     4921 tgataatgcc gtttgagtgg ctcccatgtc gtggaatctt tgagtaagag tttccaatat
     4981 cgcttgagcc gacggtactt cttctgattt tcagagtgtg aggttcgaaa acgattcatg
     5041 atctggacac gtaattgatt gaagctgcga ttgatgtgtt ggacaatatg aaagcggtca
     5101 gttacgatct ccgcatttgg aaagactgtt ttaagcaact gacaatagct ggcattcatg
     5161 tccatcacta ggtacttgac ctttaaacgg gcctttctag gaaagcgcag gaagtattga
     5221 gttagtttgt aaagtctgcg atcttcaagg atcgtaaata gctgattggt tgtcccatca
     5281 acacaaataa aactcatgga accagaaaca gaattcattg atttgaactc atccatacac
     5341 aacaccgtcg gaaggaagtt catacgtggc tggtaatgct tcgttgcttc acgcaagatc
     5401 cgtgtcaccg tcccatccga gacgaagtac ttcttagcga ttagtttacg tgattgattt
     5461 tcgtacagtt ccatgagaat cgcctgcttc agttcctttg ttagttgatg atgttcatca
     5521 actaagtccg ttttcgcaga aaaggtcctt ccacaatcat ggcagagaaa acgggtacgt
     5581 tttaattcta aaatagttaa cttttctttg actttcaaca tctgagtgcg tgtcgtatag
     5641 gaaccgttgc ggataatacg aaccgatcga cagtgttcac aataggaaga agagtcatcc
     5701 aattttcctt taataaactg cgcttgaacg cctttatatt ctttttcaat cagccattct
     5761 tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt ttctagtatg attatccatt
     5821 gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
     5881 tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaccaac
     5941 acaaaatatt atagaaccct ttttttttgc tatatgacag gaatgaaagg tgtttgcaag
     6001 cttaatgtga ggaacacttt ccatatctgt tctatactgg ataggagaag tttggaaata
     6061 tccagaaaac gaaatcaatc gaggtagcca catgaagaat aaatggataa atcgaagtaa
     6121 aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc cgtctttttc catcgtataa
     6181 agaaggatta acggcagatc aagtcaagga acgaatagaa aaaggtgcag caaataattc
     6241 agtcgatccc acattcaaaa caaatcagca aatcgtattg gaaaacatat ttacgtattt
     6301 caatttgatc tttcttatct tagccatttt gctttgttta gtagaatcct ataagaactt
     6361 gactttttta ccagttatca tagccaatac aggaatcgcc atttttcagg aaattcgatc
     6421 caaaaagatc ttggacgaac taaatgtttt aaatacagca aaagtcaatg tcatacgaga
     6481 cgggatagaa caaacaatag atatagaaga ccttgtaata gatgacattg ttattttaga
     6541 gactggatat caaattccag cagatgcaga ggtcgtggaa gggaaactgc aagttaatga
     6601 ggctttattg acaggcgaag cagatgaaat aaccaaagaa ataggtgatc cattaatgtc
     6661 cggcagtttc attgtttccg gaaaagctta cgcccgatta gacaaagttg gagcagattc
     6721 gtatatttcc cagctgacag tcaaagccaa gacaatggga gaaggagaac agtcggagat
     6781 gattgcttct cttaatcaat tgatcaaatg gattgggatc atcattattc ctatcggtat
     6841 cctgcttttt tctcaaagtt acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc
     6901 aatggaagca gcattgatcg gtatgatacc tgaaggactg tacttgctga cgacgattgc
     6961 attagcatta agtgcgacta aactagcaaa gcaaagagtt cttttacaca atatgaaaag
     7021 cattgagaca ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga
     7081 aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa caagaaataa tcagtcagtc
     7141 agatcaattg gaagcatgca ttgctgacta tgcaaaagcg atggcaggag acaatgcaac
     7201 aatggaagct gttaaagcat attttacaaa aaccactgat caatcctatc accatgttat
     7261 tcctttttct tctgtccaaa aattcagcag tgtagcgttg gcagacaagg tgtatgtttt
     7321 aggagcgccc gaaatggttt tgcgcgatca attgccggaa tatgcggaag agtttgtttc
     7381 tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga
     7441 accagtattg acagaagcag tcacaccatt aggctatatt ctgattgcta atcctatcag
     7501 aaaagaagca agagccacat ttgattactt caaaaaacag caagtagcca tcaaagtgat
     7561 ttctggagat aatccgctca ccgtatccaa tgtagctgtc caagcaggga tttcacatgc
     7621 taatcagtac gttgatgcat ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa
     7681 atatacagtt tttggacgcg tcaagccaga acaaaagaaa atcttcgttc aattgataaa
     7741 gcgtaacaat acagtagcta tgacaggtga tggcgtgaat gatattctag ccatgaaaga
     7801 agcagactgc agtatagcga tggcttccgg aaatgaagcc gccatgcagg cctcacaagt
     7861 ggtcttactt gattctgact tttcacgcat gccagaagtt gttgcagaag gacggcgcgt
     7921 tgtgaacaat attgagcgat cagccagctt gtttttagtg aagaatattt tctccttttt
     7981 gctctcactg ttttcagtga tctttgctct gacctatcct ttagaaccat cacaaatcac
     8041 attgattagt ttgtttacta tcggacttcc atctttcttg cttgctctag aagaaaacaa
     8101 aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc caggtggact
     8161 aacagatatg atcgtagtcg gtgcgttggt gatctgcggg gtgactttga atctaaataa
     8221 aacagatgtt tctacagcat ctaccatgct tttgattgca gtagggttcc tcgttctcta
     8281 taaaatctgt tcaccgctga ataaattcag aagtcagatc attcttttct gtgctagcgg
     8341 taactttttt tcggtcatct ttttgcataa gctgttttca atcaccggta tctcagctgt
     8401 atcacttttg ctgttgagca ttctattttt ctcggcagat tcgattttta ggcatttgac
     8461 cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg
     8521 cgtagctaaa aaatttctat cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa
     8581 aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc aggatgagtc atccaacttt
     8641 aaaacgcacg agcagttgct cttttttaca ttttcggaat ggtaacggtg aaagtcgaac
     8701 ctgttccaag atgactgtct acagtaatcg taccattaag gagttgcacg tagtctttta
     8761 caatcgctaa ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt
     8821 aaaaacgctc aaagatccgt tcctgatcct caatgtcgat acctatacca aagtcttgga
     8881 cagaaaacga tagatcatct gttgcctgat agcggataat aatctcgctg tcgctttttg
     8941 aatattggac agcattttca atcagatttt tgataattgg ataaaggata tctgttttag
     9001 tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg
     9061 gctgataatt ttgaacgatt tgctggaaat aaggttctgt ataaattgtc tggatctcgt
     9121 aagaattctc gccatctttt gacagctgga tgatttcccg aatcagttta tctaaccgga
     9181 tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac
     9241 catccagtaa agtctcggta aaaccaatca gtgaagtcac aggagttttt aattcgtgag
     9301 agacgttgcc aacgaaatcc ttttgtagct tttctaaacg tcgcactttc gttaagtcat
     9361 acgccacccc tagaatctgt ccggtacctt ctgaattagt aaagtagcgc aaactcaagt
     9421 ctaatgtctg ccgaccttct gtaagggtga tctcctgatg gatcagcgga tgctcaggag
     9481 tcacttgatg aatcagctga atgagttttg gctcttgaat gacttctgtg taccgctgtt
     9541 caggttgata gcttaccacg tttaaatgta gctgcatctt tggattaagc agtagtaaac
     9601 gagaatcagc atcatcaatc aggaatacgc caatcatcag ttcgttcagt aacgtatata
     9661 attgctcttc tgtggtagta cacgcccgat acatcttact catttgttcg ctcaacgcat
     9721 taatcgtctg atacaattct tcccattgat cgggtgtttg catgattact tccgcctgat
     9781 taggattttg caccattttt tttagtacgg gcagcacggt tttcaatggc tggttttttt
     9841 gatagatcag ataatagatc attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa
     9901 agacccaacg gcgaaaactc tcagttctcg gtaaaaaaac gcttgtcggt tctgcgatac
     9961 gtaaaatggc ttctaactgg ccactttttt tgactggtaa tgcgacatat aaaagatcct
    10021 cattcaaagt agtacttttg cgcaaagcgc tacctaggtt cccaccattc aatacggctt
    10081 tgatttcggg acgggagttt cgctgttcat tcaatgtttc attgtttgtg tcaaataaga
    10141 tctttccagt agcatccatt aaagtaatcc gttcgttcga ctgatggacg aattcttcca
    10201 aagcagtttt gttttgcgct gcttggaggt tttcgacatc taattgccgg atcagcagtt
    10261 gtgctttttt tgtcaggtag ctttcttgct gttccacgat ctgctgttga aaataatggg
    10321 aaatcaattg ccagccacca aaaaacaaac taactaaaag aataagaaag aagagacgtt
    10381 cataaatttt tttccatttc atcgtttggg ttcctgaaac ttatagccaa atcctctaat
    10441 agtcaccaag tactttgggt gttttggatc tcgctcgatt ttttctcgca aatgactaac
    10501 gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa
    10561 tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa taaacaagta attcaaattc
    10621 tttcggtgtt agttcaatca gatggtcgtc tacggtcact tgataattgg tcaaatcagc
    10681 agtgatttgt ccaatcgata gatattcagg ctcagcttca tcttttgatt gcttacgagg
    10741 ttcaattcgt ctgaatattg ctttcatacg ggctaatact tcgcgaggac taaatggttt
    10801 ggtcaaatag tcgtcagcac ctatttctag gccaatgatc cgatccactt ggtcatcttt
    10861 agccgtcagc atcaaaatcg gtgtttcatt tttttcttga cgcagccgct gagtgatctc
    10921 catgccatcc atcgttggaa gcatcacatc taaaatgatg aaatcaaatg attgttctaa
    10981 tgctaattct aatccttcgc ctccatctgt ggcactggtt accttgtagc cttctttttc
    11041 caaattgaac gtcaagagcg ttacaataga aggttcatca tcaacaacta gtattttttt
    11101 cattcaaatc tcctttaaat aggtttcatt ctgtttatat tttagcatag aatcagtaca
    11161 aagggctatt ttacaaaaga aataaatggt ataataggaa ggcactttgc tgaatagaaa
    11221 ggaagagcag tcaatgattg gaatcagtgc atgtttaggc ggtctggcct gtcgctatga
    11281 tggacagaaa aaagaagtac cagaattgat ggaactagtt gaaactggag aagctgtcat
    11341 ggtctgtcca gaagtgatcg gcggattacc aattcctaga gatcccgctg aaatcattgg
    11401 tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa gcggcgaaga
    11461 tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat caaaaattgc aggaaaaaca
    11521 cattgatcta gtcatcatga aggaaaaaag cccttcatgc ggaacccatt cgatttatga
    11581 cggaagtttt tccggaataa aaaaaaatgg tgccggagtg gctactgctt attttattca
    11641 gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa actagaggtg agcagaatgg
    11701 aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt ttattcgacc ttattgacag
    11761 aaaaacaaat gaactacatg gaattatatt atgcagatga tttttcatta ggtgagatcg
    11821 cggaagaata cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg acaagcaaga
    11881 tcttggagga ttatgaaaaa aaactccatc ttttttctaa ttatattgtt cgagaacagg
    11941 tattggaaaa tatgactgca tatattacag ataagtaccc ggaagataaa aaactacttg
    12001 aatatgtaca gcaaattcaa gcaatcgaag aataaataaa aggaatgaag aaaatatggc
    12061 atttgaaagc ttaactgaac gtctccagca ggcgatggga aaaataagaa aaaaaggaaa
    12121 agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct tactagaagc
    12181 cgatgttaac ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg
    12241 ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga
    12301 attgacgatc acacttggtt ctgaaacagc agagttaaat aaatcaccaa aaataccaac
    12361 tgttatcatg atggctggtc ttcaaggggc aggtaaaacg acctttactg gtaaattagc
    12421 caattattta aagaaaaatg aaaatgctcg tccgctccta atcgcagggg acgtatatcg
    12481 accagctgcg atcgaccaat tgaaagtatt aggtcaacag ctagacgttc ctgtatttga
    12541 tatgggaact gaagtcagcc cggtagagat cgtccgtcaa gggatggagt tagcaaaaga
    12601 aaagaaaaac gactatgtat tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt
    12661 gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt tgcttgttgt
    12721 cgatgccatg actggtcaag atgccgtcaa cgtagctgat agcttcaacc agcaattagg
    12781 tattacaggg gtcgtcatca ctaaacttga tggggatact cgaggcgggg cagcactatc
    12841 gatccgctca gtgacaggcg caccaatcaa attcatcggt tctggtgaaa aattgactga
    12901 cttagaagta ttccacccag atcgtatggc tagccgtatc cttggtatgg gggatatgct
    12961 gacattgatt gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag aacttgcaca
    13021 gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt gaacagcttg atcaagtgat
    13081 gggtatgggc ccactggaag atctaatcaa aatgatccca ggaatgaatc aagtccctgg
    13141 aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga tggtttattc
    13201 tatgacgcca gcagaaagag caaatcctga tctgttgaac ccaagccgtc gtcgccgaat
    13261 tgctgccggt tctggaaaca gtgttgttga agtgaatcga atgatcaaac aattcaagga
    13321 atcaagaaaa atgatgcagc aaatgtccaa aggggatatg aacatcccag gtatggacca
    13381 aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa
    13441 gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct tcggtacaga
    13501 ctgaaaatgt ctactgccgg agcttttcta tcagcaattt ttattcttta ctaatacaat
    13561 tactctaaac gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa cagaaagtat
    13621 gcggcaatat catgatactg tctggggtgt tcctgaatat gatgaccaaa agctgttccg
    13681 aaaattgatg cttgatatta atcaagccgg attgagctgg cagacgattt taaataaaag
    13741 tgaagcattc gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa attatggaga
    13801 aacaaaaatc agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga
    13861 agcagccgtc caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc
    13921 agagtatctt tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc attcacaaga
    13981 agaagtccca acaacaagtc ctctttcaga tgaattagca aaagatctaa agaaaagagg
    14041 atttaaattt atcggaagca caacaatcta tgctttttta gaagctgtcg ggatcatcaa
    14101 tgatcacctt gacagttgtt tccgcaaaga agaggtcgta cgtatcggca gaaagggcga
    14161 ataaagcatg cgaaaaatca tattttatgg tgctataagt ttagatggct atttggcaac
    14221 gaaaacggat gatcttcaat ggctttttga cactccaaca ggtaagcaaa ctacttatga
    14281 agctttttat gaaacaatcg atacgaccat catgggcaga aaaacttatc aggaagcaaa
    14341 aaaatatttg gagaccgaag caatttatcc agagaaaaag aatttcgttt tttccaccca
    14401 cgcacaatta caattgccag atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa
    14461 aaatctacaa atgacagaag gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa
    14521 accgctattg gaacatcaaa tgattgatga atggtatatc cagattaccc ctgtattatt
    14581 aggggacggg attcctttat tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga
    14641 cacgacacga ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga agtaaataaa
    14701 ataaaaaagg caactatatg cagaaatgtc ctgtacatag ttgccttttt tattaccgtt
    14761 atgcttttta ctctgttaat ttgttagaaa aatctagata cgcatctaaa atcgtttgga
    14821 agaaacttaa tgttccttca acgatattac cattttcatc aattagattc gcaacgtttc
    14881 cgatatatgc ttccggttgc tgcaaggttg gcacattcaa aaagacaagt gattggcgta
    14941 gatggtggtt tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg actaaacctg
    15001 gtttatgatc ccaaacactg tgtccataag gtcttgagcc tacatctaaa gcatttttca
    15061 atactgctgg cacagaacgg ttgtattcag gagtaacgaa aacgacaccg tctagtcccc
    15121 tcacttcctc acgaaaacga gtccattcag caggagcctg ttctggagtt tcgagatcct
    15181 cattgtaaaa tggcagatca tcgattttta caaatacact ttcgtaccct tcaggaagca
    15241 atttcgcaaa agcttctgct acttttttgt tgtaagaatc ttttcttaaa ctaccaataa
    15301 aaaatccaat tttttttgtc atacgatttt tcctccttca aactatctat aataatctta
    15361 caaaagataa gtaactggag caaataaaaa cacttactga caaaagctac atagaagata
    15421 gattacttat ttttaacaaa aaagaagtct aaaatcttct attttctaaa aaaaatcgaa
    15481 tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt tacactacat
    15541 cttgtgaata aaacaatgga ggtggaaata aataatggca gttaaaatcc gtttaaaacg
    15601 tatgggttct aaaaagagtc cgttttaccg tatcgtcgta gctgattcac gttctcctcg
    15661 tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt
    15721 agttttaaaa gaagacttag ttcttgactg gttatcaaaa ggtgcacaac cttctgacac
    15781 tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc
    15841 aaagaaataa ggtgaccgga tatgaaagat ttaagcgact tagtcttaac aatcgttcgt
    15901 ccgctagtca cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa agacttttac
    15961 gagtacaatc taacagtggc tcctgaagat attggccgta tcattggaaa acaagggcgt
    16021 gttgctaaag caatccgcac aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg
    16081 cgtttgaata tcatcgatga taaggaaaag taaataaacg tgctgtactc gaattttcga
    16141 gtgcagcttt tttgtttttt ttattaattg aagaaatgat tatttggcaa aacaagcaag
    16201 aaagcgtatc cttataggag gagggagtga acagccaatg agaaagaaaa tgttatttat
    16261 tccatttgct ctattgttaa gcgcttgtag ttcggttact caagaatcag acaatacaca
    16321 tctatcaagc agtatatctg aaactgttac cagtccagaa aaaagtagta ccaaaacggc
    16381 ttccaccact gaacaaacaa tggctacctc aaataatgag aaaaagactg ctttagacca
    16441 gttgaaagaa caacagccaa atgttcccat gcctctagat gtaccagtct cttcaggcta
    16501 tctcaatatc gcagcgacac atacaaagca aggttattct attttatatt acagaacaga
    16561 tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa acgcctattg caacctatct
    16621 atatcaatat gggtttgctt ctagtcaaga aacaatccaa gttttgcagc catttgagat
    16681 cgacacaaat ggtcagcaag tggacttagg ttctcgaatc acagggtatc agcaaggtgc
    16741 agcaggttca agttttcttg aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa
    16801 taatatagaa ggccaagatc ccttactatt agctaaagaa atcgtagctt atttggaaga
    16861 aaacagtcta ccagcacctg aacaatttgg taaaattact gttgatatgg gtgatacgac
    16921 gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat gcctatacga tcactcatca
    16981 agatcctatg tctgcgttga agatggcagt ttctatgaag aggttgtgaa atcagcgcgc
    17041 tgatctgagc ccatcattta actccaagtc acaggaagta atcaaaaata gcacgccgca
    17101 tttttgcata attttggctt aataccttaa aagaaaacta gaattgcctc ttcacacatg
    17161 ctaaaataaa acagacaaaa aagaaatggg gatataagtt gactgaatac ttgaatgtag
    17221 gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg tgttatctca acaacggatt
    17281 tcccagaaga acgttacaaa aaaggaacgg tgctaacact attccaagac ggaaaagcac
    17341 ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa ttttgactta ttgagttttg
    17401 aaggacatcc ttctatcaat gatgtggaaa aatatcggga tggcatatta aaagtgtcaa
    17461 aagacaactc tgtagatcta gctgaaaatg aattctatta ccatgagatc atcggtctgc
    17521 atgtattaga agatggaact gagttaggta aagtaaaaga aatcttgtca ccaggtgcca
    17581 atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca tatatcgctt
    17641 ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg tgtagaaatt ccagaaggac
    17701 tgattgacga tgaagattga tgtattgact ttatttccaa gaatgtttga aggtcctatg
    17761 ggggaatcca tcatcggtaa ggctgtagat aaaggattat tagatatcaa tatctctaac
    17821 ttccgcgatt attcagataa caaacatcag acagtagatg attatccata cggcggtgga
    17881 gcaggtatgc tgctcaaagt ccagcccatc tatgataaca tccaagccat tgaaaaagct
    17941 gcaccggatg tcaaaaaaag agtaattctt cttgacccag ctggtaaacg attcgatcag
    18001 aaaatggcag aagaattttc acaagaagaa cacttagtat tcatttgtgg tcattatgag
    18061 ggatatgatg aacgtatccg cacattagtg acagatgaag tttccttagg agattatgtc
    18121 cttactgggg gagaattagg tgcgatggtc atgatcgatg caacagttcg gcttttgcca
    18181 gatgtgttag ggaatcagac ttcagctcaa acggattctt attcaaccgg tttattggaa
    18241 catccccaat atactcgccc ggcagaatat aaagggatga aagtaccaga agtattgaca
    18301 aatggaaatc ataaattgat tgaagaatgg caattaaaag aatcactaag aaggacctat
    18361 caacgtcggc ctgatctttt ggaaacactt gaatggacac ctcaaatgac gaaattttta
    18421 gaagaaatca agaaagaaga acaagaaaaa acagcagatg aagccaagtg actttttcta
    18481 aaatcttctt tacagtaggt tgataatatg ttagtatttt atggtgagtg caaaagcact
    18541 taactattac gatattccgc tgtgagaaaa gctcataaga atatttggaa taaggagaag
    18601 aaaaccatga atccattaat cgaagaatta acaaaagaac aattacgttc tgacatccca
    18661 gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa
    18721 cgtatccagt tatttgaagg tgttgttatc aaacgccgcg gtgctggaat cagcgaaact
    18781 tatacagtac gtaaagtttc taacggagtg ggcgttgaac gtacattccc attgcataca
    18841 ccacgtgttg caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac
    18901 tacctacgtg cattacacgg aaaagctgct cgtatcaaag aaatccgtcg ttaatcatcg
    18961 ttatcagaca gagaaagaac ccttggtatt caagggttct ttttttatta tctgtttttt
    19021 tatattaacg atttctattt ggtacatttt tgattaataa aaaggggcaa aaaaggggca
    19081 acaagttaca gtttttctat atcttctttc aatttttctt ttactccttt tgtcacatgc
    19141 aaataaattt tttcagtgat atcactattt tcgtgaccaa cgcgatcctg aatggcatac
    19201 agaggagttc ctagttccgc taattttgaa atatgggtat gtctaaaaat atgtgaacta
    19261 agttttttat caatcttcat gtcagcttta tgacttctca aataggtgtt aattgctgtt
    19321 agttggaaag gagttccttt cgttgtttga aatagaaatt gaccatttgg atttaattct
    19381 agaagctcat tgtaaatagc tatcgctttt tttggtaaat caatttctcg cattccggca
    19441 gctgtttttg tagaatcaga ttttttcata tcagctattg aacgttctct atacatcatc
    19501 gtcccattta taactactga tgcattatta ttggtaatgt gtacatcatc tttacttaat
    19561 gcgattgctt caccagacct catcccagtc aaatatagcc actggaaaag gagagaatat
    19621 cttttattgt gtgaagttgt gaaatctact agtctattat attcatcatc ttctaaaaat
    19681 ttatctttaa tttttatcgt ttttgactct cttttataat caataactac ttcatcgata
    19741 ggattttttt ctacataacc ttttttcata gcatatgaaa aaagaagatt caatttggat
    19801 ttaattacac tgacgtactt atttgacaag tcatctttat atatcatgtc ttcaaaaata
    19861 ttattcaaat ctattgtatt aataccagaa acgatgtaag tctcaggtat cttcttcttt
    19921 atagtgttta atatattatt cgtaggataa tatgtagatt ccttaacttg tcttttgtat
    19981 ataactaacc attcttcaac aagctcatga aatgttagat caggcttatg aagtgttttt
    20041 tcgtttaatt ttatatcaat ttttttattt aactctagca atgccacttt ctgagtttct
    20101 cgagatttat ttttataagt aatgctcact ttttttcttt ttctagtttt tggatcaata
    20161 tatcgctcat tatatttata tacttttttt ccgtttttat cagttttagt ttcgatccac
    20221 attgttttca tctcctattt gttgctataa taggaataga taagtaagcc gattatagca
    20281 ggtttatttt tcatcacgtc cacaaacttt ggcgagggag gggcgtgttt ttgttagtta
    20341 ttgtggtagg ttatctaaag cgtattgtgc ttgctcattt gtaaaacctt caaatataag
    20401 ttgatcgtac aattgcgaat ctgacattga tgcccaatca tcgtaacttt ccgctttagc
    20461 caaagcttgt tcgttgtaat cgacatcaac attagccagc gcataatcga ttgcatcact
    20521 tggatattct tcaaactcta gttgttcacg caaaccttgt tcagacatac cagcccaacc
    20581 tagatagctt tcagctttgc tcaatgcatt tctgtattca cgaggaacac tttcttcttg
    20641 agtaacttct tctatagtag tttcagtagt ttcagtttct tcttcaacga ctgtagattc
    20701 tgctaccgat tctgcagaag acacttctgt cacgctttct ttagttgttt cagcgacggt
    20761 atttttagca tcatcactgc ctccttgcga acctatagca ataaacacca caacaaccgc
    20821 taacaaccaa aaccataccc gtttataaaa cggtttctta actttataca ttttcccatc
    20881 ttgacccata actttttttg ccatttaaat atccctcgct tcttgttata atatatttgt
    20941 gatctcagaa atgaggtatg agtccgtgtt gcagcacggg cttttttctt tataactttt
    21001 ttagagatta taggcaaaat agtagggcat aaaaatgtat tattgaattc cgtatttaga
    21061 aaatcctaat tgaacttcac cggaagtctt ttgctgtgtt gtacgcaatg cttcttcttc
    21121 agacattcca ttctgtactt tccatgcaac aggcgacatc ccgtatttgt taacaaaatc
    21181 agtaagtgat aaagtgtcag cgtcttgctg agcgcttgtt tgttggtctt ctggattctg
    21241 ttgagatgct gcttgttgtt ctttctgatc ttgactgata atattgccag catcatctgt
    21301 agtcaatcca ttttcataaa gggccacgcc gaaagcttcc cactctttgt tggaccaatt
    21361 tgcacgatca gctggagttg actgtaaagt gcgttgtttc atctgttcat atgtttcttc
    21421 ttgaggtgcg gtttggattg tactctgact ggagcttata actgttgggc taggttccgc
    21481 cgtagcttgg ttggagcttg taactgttga actagaatct gttttagatg tagatttgct
    21541 agtagatgaa ctggtttcag ttgtttcttt tgttttactt atttttgttt cttggttaga
    21601 agtggtatct gttgattcag cttttttatt atttgaacaa gctgaaagta gcagagcagt
    21661 acttaacaac aacataacgc taactttttt cattttataa ttcctctttc tcgttgtaat
    21721 atgtgtgcta acacgggctt ttttatataa gaaaacgata agcgctttct ggaagcccgt
    21781 aaagattctt taattcctcg atttttttag gatattgatc attatcttct ttataaagag
    21841 aaacaatgag attagcagca aagcaattag cttcgctttc agatttgctt ctagatgttc
    21901 ttgttgatac ataatagctg gataagccac gatgaaaaat agcgtgacct aattcgtgag
    21961 cgcaaatgta gaatctttcc tcagagtctc gcagttcatc atttaaaaag attatcgcac
    22021 gatctctaat ttcttgaaac tgccccttgg gattctcgat aaaaggaacg tattgaattt
    22081 taatgcccat cttttcacaa atataaaaag gattagcgga ctggtatttc cgcttcaact
    22141 cctcgactaa attaatcgta tccatctcca taagctcaca tctttttgcc tttttctttg
    22201 tcttctttca caatatccca gaaagtcgct atcaggatat cttttacgcg ctgtatttgt
    22261 tcgggtgtca atgtttcccc accataagac atattaacat ttgagtctag tagtttatca
    22321 agttcaacca cttcctcttt tgtagcccac ttgggaacat tattatttcc caataaataa
    22381 tcagttgtga caccaaaata atcagcaact ttctttaagt tctcagattt tggcgaggct
    22441 ttatcccatc ttcttatttg tccattagag atgcccacct gtctttctac ttctgctata
    22501 gtcacatgct tttcgtctgc taattcttta atcttagtaa ctaaactcat tattatcaac
    22561 ctttcaaagc tgaaaagaaa ataaatagct taaaagttat atttttggtt gacaattagc
    22621 ttttaagcta gtatatttaa ttcgtaagct aaattgttag ctaaataaga gcaacaaaaa
    22681 actctactaa tttaaaacat tctctcggtc gccaaactta gaaatgttat tttagaggct
    22741 ttttataagt cttatttaac tatgtattca taatagctta aaagctaata ggtgtcaacg
    22801 atttagctaa ttttttagct tacaaattat ttgtttagaa aggagctatt tttatgtctg
    22861 agaatttaga cttaaaaatt cgagcggaga tgagaaaaag aagaatgact ttcaaagaac
    22921 tagctgcgct tatcggtatt tcaggagctt atttatcaga tattctaaac ggcaatcgtg
    22981 atggaaagaa agcacaacag catatcgaaa cagtgaaaaa aatattggac atccgatagg
    23041 gggggtaggc gatgacaaaa ctaaaaaaac aagattttgt aaaaaaatat aattattctc
    23101 catctactta tcaacgtcga atgtcggaac taaaaaatac agcaattttc tcagcggcgt
    23161 atgaacgggt cacaggacaa gaagtttgga tcaatacaga attatacgat aaatttttgt
    23221 ctttcaaatc ctataacagg ttacgcacaa gaaaggtaac acctaaagaa tttatcgaga
    23281 agcatttagt tgatttataa aaaacagaac attttgagag gtgaaggtta atgggtaaat
    23341 tcaacagagc attagtattc agcgcaccgc taatcgtcta tgctttaggg ctttggggaa
    23401 gtagacaagc gttgatagga acgattgtgt acatggtttg gatttttata gggctggatg
    23461 aagctgagta caaaacaaaa aagccagtcg ggagggactg actaatgagt aaggacgata
    23521 ttgtatttct gttaattatg ttttgtggga ttggatcaat aataattaat ttgtatttga
    23581 ttattaaaga aaaatttttc aattaaaatg agtcttttag ctttaactta aatttttttt
    23641 gataattagc tgttcttatg accattacaa gtttttcacc aggtaatata tctttcatat
    23701 ctaaaacttc tgaaaaagcg actgagtagc caggagagat tgcttgtgcc aacggtatag
    23761 gagataaaag gctactttta tgaatgcttc ccatatcaaa accattggcg tgataatgtg
    23821 aacaattaat tttcttaccg ttgtaaagat acgtacattc gataattgag tttggcattt
    23881 ttgatgggtt tgcaattacg ccagtaagaa tcattcgatt acttctatca tgcttaacta
    23941 aaaaatagga gagcgtaatt tttcttttat ctttaaataa gttatagagg gataaaaaaa
    24001 atcctgaaat agaaatgatt aaagtaatag cgacttggta atcgctcaaa atttttatca
    24061 agtaattcac caccttttta ctcaaattat atcaaaaagg agagaagaaa taatgcaaga
    24121 attagtaatt ttgaaaaata aaaaaagaca cgaacgccgg caagcatgat cgtgtcaggg
    24181 aaataaacta tctttctata ttttaccaca gaaaggaatg aaccgtaaat gctaattgca
    24241 acggatacac tagaccgcat ctttttaaaa gacgaataca aactgcgcaa tatagatgcg
    24301 tcaggaattt tagtatttga tctttatgac aatggaaaaa ttggtatcta tcaagcaagt
    24361 gatattgaag aaacaaacct tgctttcgaa caaattgatg attctgtgga attggattta
    24421 gatgaggcaa tcctagcttt tgaacaaatt gcaaaattat taaaggaggc acaaaagaat
    24481 ggcaactctt taccaactca gcgagtcata tatcaaagtc ctggaactgg cagaacaatt
    24541 ggatgaagaa attcttcgcg atactctcga ttcgattaat gaagcgatcg agtataaggc
    24601 agaaaactta gcaaaaatag ttaaagaagt agaagggaaa gctgagttaa tcgatagtga
    24661 aattaaacgt ctgcaggaac gtaagacatc gcttttgaac aatgctaaga gtatcaagca
    24721 ctatttacaa gaggaaatgg aaaagactgg taaaacgaag attaaaggtg aattattcaa
    24781 cattggaatt caaaataatc cagtatcggt caatgtaatc aatgagaact taattccaaa
    24841 aggattcttt acccctgtgc ctcccaaatt ggataaaaag caattgaagg aggaactgaa
    24901 gcacggagat attcctggtg ctgaactcgt ccaaacaaaa ggtttgagga taagatgatg
    24961 aaggaggaga aataatggaa cacgaaaacg taaaagacgc tttgaaagct gctatcgaaa
    25021 tcgcggaagc taaaggaata aaggttgacg gcaaaccagc aactgtccat gacattcaaa
    25081 atttgacgaa agaacatctt tattttatcg ccgacttgct tggtttgtcg gaattgtatt
    25141 tagacaaata accccacacg gaaggagtgg ctagggtggt tgtattacaa gtaatagtct
    25201 gcgtaacatt catttggtcg tgcataccgg tttgtatata tatcatgaag aagcttcgcc
    25261 ctgagtctta caaattatac gcaatttaca gcataacagt agctcttgca tttacattgt
    25321 ttgcaatttc taggcttccg ttttaatcgt gggaatgaat ttttaatgaa ctaaattatc
    25381 tataaagcaa aaaaaggaga aacaacattg aaaaacaaac tagcaaaaac aacagcaatc
    25441 atcggactag cactaggtag cggcgttatc ggctacgcag caagcaatgc gtttcaagat
    25501 ttggacacga tcaaagcaaa tttcaacaca gtcctacaat acggtcaaac gaaatcgcaa
    25561 cgtgtgtcag aacttgaatc acagctatcc aacaacactc gcacacagga gcagctgaaa
    25621 gccgaaattg agcaaatcaa atcggacaag cagaaggaaa ttgaagccaa gcaacgagaa
    25681 gtcgaacaaa agcaacagga gatcgctaca aagcaacagg aagccgatag cttgcgccaa
    25741 caactatcca cagtgcaaaa cgacaaggag cagttagaac agcgcgtgag cgagttacgg
    25801 cagtatacgg atcaaaaggt aggggagttg gggaaatgaa agagttgact atttatgacc
    25861 caaatatttc atgggctaaa cacacaatta aagtttcttt tatgatttgg ggttacaaag
    25921 gatatgttac ttacaaagtg ggcggtaaca ctaagggttt atcattaata gctattgatt
    25981 cagatgatct atatgatgct aatttcgagg ataacccagt taatttccgt gaccttgatg
    26041 aagattggtt ctctatggaa cttactaacg ataaaggcga cagtacactt gtagaagatg
    26101 aattcgacag actaggtgat tatattgtcg gtgttgaaat tattgcgcac gaaccagagt
    26161 aggagagtga atacaaaatg attaaaagaa tcactatatc agttgaagaa gatagtggat
    26221 caacaatgca gcataccgac aatcgaaaaa cttttttaac caatagaaag gagaattttg
    26281 atgcagatta agaaggcgac ggatatcgaa caaaccaagg gaacatattt gatctacggt
    26341 gctccaggaa aaggtaaaac ttcaacggct aaatattttc cagggaaaac actgatcctt
    26401 gatattgatc ggacttcaaa agtccttaga ggtgagaaaa atgtcgatat cgtatatatc
    26461 gacaacgaag atacctggaa cgactggggc aacacgttgg ctgatttaac taccaattat
    26521 gtaggtgttt atgacaacgt ggttgtcgat aatgtcagcg aattggaacg gtgtattctg
    26581 tccagtttag gcgcagaagg aaaaaacaac ggcgttcctt cgcaaggcga ttatcaatat
    26641 atgcaattta gaatggtgaa cagcctacgc tatatgaaaa atttagacag caaccttatc
    26701 tggaccgcat gggaagaaat cgatttgtgg acggattcgg atggatcttc ttacaacatc
    26761 gctttgccgc agatcaatcg aaagattcga aacaatattt tgggattatg tgacgttgtt
    26821 ggtcgattga tggtaaagga agacgatgaa cgcggattta ttttacaagc cacagattca
    26881 acctatgcca aaaatcaatt ggataataga tctggctgta agcaaagtga gctgatttta
    26941 gatggaattg tacgactacc agaatgattt agtcgatcgt gcaagacaag cctatgttga
    27001 tggttacaag gcaccatgta tcgtatcacc gtgcggttct ggaaaatcag tcatggttgc
    27061 agagatcgct aaacgaacca cacgaaaagg caacagagtg ttgttcttgg tccatcgtcg
    27121 tgaattgatt gatcagatca aagatacgtt tcaaaagatg ggcgtagata atcagcttgt
    27181 aaatttcggc atggttcaaa cagtcgtacg acatcttaaa accatcaaga aaccacaact
    27241 gatcatcact gacgaaaacc atcacgggtt agcagcgtcc tacagaaaaa tctatgagta
    27301 ttattcggac gttccgcgat taggttttac cgcgacaccc atccggttaa acggtagtgg
    27361 gttgggggat gtgaacgact tgctaattga aggagtatcg gctaaatggt tgattgagaa
    27421 tcatagatta gctccttatg aatattatgc acctaaattg atcgatacag ccgaattaaa
    27481 gaaagcttcg actggagatt ttacaaagaa atcaatggat aaggcagtga agaatactat
    27541 ttatggcgac gtgttgaaac attataaaac gttagcagaa ggtgaacagg caatagcata
    27601 ctgtcacagt atcgatgcaa gtaaacacac tgctgaaata ttcaacgatg ctggttataa
    27661 agcaacacat atcgatgcaa aaactccaaa agatgaacgt gcagatatta tcgaagcgtt
    27721 cagaaatcat gaaattaaaa tcctatgcaa tgttgatttg atcggagaag ggtttgatgt
    27781 tcctgattgt tcgactgtaa ttatgttgag acctactcaa tcgttatcat tgtacattca
    27841 gcaatcgatg agggggatgc gttaccgacc agataaaacg tcaatcatca ttgaccatgt
    27901 gggtaatgtg aaccgttttg gattgcctga tatggatcgt gaatggagtt tagatacaaa
    27961 aaagaaaaca aaaactgata gtgatttatc tatcgttcaa tgcgcgtttt gttttggtgc
    28021 ttacgaacgg ccaaaaggcg ataatatttg tccgtattgc ggagagttgc agccgattga
    28081 agaacgaaag tcagagttag aaatcgatga atcggctgaa ttaatgaaag tcggagaaac
    28141 gaaaattact ttgaattttg agaacaataa atattacaac atgacggaag atgaagcaga
    28201 aagtatagag gacttatatg ccatagcaag agctaaagga tttaaacctg gttgggctta
    28261 tatggctgct aagaggaagg ggtggttgta ggaatgggcg gtatttttgg taaaaaagca
    28321 gaagatgttc aaggttcaat aataaatggc gtaacaataa taggtgatac tggaaaaaga
    28381 gatagttcag gcaatcaaat tgtaatcgct agaaataaaa ataacgaagt ttttgaatct
    28441 tctctatcta atataaaaag aggtgttgtt aacgggacag gcgaagagag tggctacgtt
    28501 aatttaaaaa aagctaatga aaaataccat gttgaaggaa cttacgttaa ggctttgtct
    28561 gtcggtggaa caaaaggcgt taattgggat aaacgaagaa ataaatggaa agcacgatta
    28621 tatttcaaag gagaatatgt cttacaaaag gatttcaaca ccaaagaaga agcaatccaa
    28681 gcacgacttg cagcagaaga aaaatatttc aaacctatct tagaaaaata tgaaaataaa
    28741 aaggagaatg attaacatga caggatttaa cttagacttt tcaaaagcgc aacaagggaa
    28801 cgaaatcaaa gacggtacgt acgaagtggt cgtaaacaaa gctgtagaaa atgcaactaa
    28861 atcaggcgca gaatttatcg atatcgattt gatcgttcgt aacgatgtgg atcaaccgtt
    28921 ccaaaataaa cacattttcg caaaaatctg gaaagccaaa gctaccggca aatataacga
    28981 aggtatgatc atggcaattg cacaagcgct acagttggaa gacggaaaat catataacgg
    29041 gtttgatgaa ttgctggcgg actttgtttt gaaaaccgcc tcagtacgcg tgaaaactga
    29101 agagtcaaat ggatataaaa acgtcaatgt gaagtcttgg gataaaacga acacacgggg
    29161 cgtaatgaat caccaattca aaaatggtga tgaaccatcc ttcggaccgg aacgttcaag
    29221 agcgacaacg gttagaaatg acaatcttcc attttaattg aggtgtaatc aatgtatgaa
    29281 aaaattccaa aagagcttaa aaatctcaaa caatggtgtg tttataagct agtttgggat
    29341 gaaaagcggc agaagcacac gaagatccct tataacgcca acaacgggca taaagccaaa
    29401 tccaatgacg agagtacctg gtctgatttt cagaccgctc tcgctgctat tgatcactac
    29461 ggaatgagtg gactggggtt ctttttcaaa ccaccttatt tcggcattga tatcgataat
    29521 gctgaaggtg aagttgagcg atttaaatca ggtgatatag aagaaaatat tatctatgaa
    29581 tttatcgaaa gcatgagatc ctatgctgag tattctcaat caggtactgg catccacatt
    29641 attgctcgcg gagaattgcc cggcggaaga cgcaggaaag gtgacgtaga aatgtaccag
    29701 aacggccgat tcttcgttat gactggaaat gccgcatcaa aatatttaga aataactgag
    29761 ccgaatccaa aggatatcaa acgcctatat gatcgctacg ttggagatga aaaaatcatc
    29821 caattcaaag aagaaaatcc gctgatgaat accgttgatt taccggttga agaaattatc
    29881 aaacgtgctg aatcgtcttc ccaaggggca agattcaaag tctttatgaa cggcggttgg
    29941 gaagcttttt attcctcgca atccgaagcg gatatggcgt tcgccaacga cttggctttt
    30001 tggaccggta gagactttga aaaaatggat gagattttca gagcgtctgc aatgatccgt
    30061 agcaaatatg accaaaaaag gggatcggca acttacggag aatctctact aaataaggca
    30121 attgctgata caaattccgt ctacaacccc aaacggaaat ctgattttaa gatttttatc
    30181 aaagaccagg aacagccaaa agaggaaaaa tactattcgt acgacgacac cggcaacgcg
    30241 gatcggttta ctgatatcta cggaacgtta gtcaaatatt cgtacattga taaatcttgg
    30301 tattactacg atgggaaagt ttggctacag gacaataccg gcgaagttcg gaaaatgatc
    30361 gatacgacag tagacatcat gggaaaagaa ccgttaacga tccctgaagg cgcagacgat
    30421 gaaaccaaag aagctttgat gaaagcgaaa gaaaaacacg tcaagcgttc tcgcagcaat
    30481 gccggaaaaa acgctatgat ggacgaattg aaacatcgac tatccgtatt gccggaagag
    30541 ttcgacaaag ataaaacgct gttcaacaca caaagcggct atctgtcttt gcatgacggt
    30601 cttctacacg agcatgagat agacaaaatg tttacacgtg tttcaaatgt cgagtacaca
    30661 gaatcggtcg attgtcctat gtgggaagaa tttattaatc agatttttga caatgatcaa
    30721 gaattaatca gatatatcca gaaatgtgtc ggatattccc tgaccggatc taccagagag
    30781 caatgtatgt tcatcctcta cggtcatggg tcaaatggaa agtcggtatt tttggaaatt
    30841 atttctgaat tgatgggaaa ctatgcgatg actatgcagg cacaaacgat catggtcaag
    30901 caaagccaat catcagcaaa cagtgatatt gcacgattga aaggtgcgcg gttggtcact
    30961 tcgtcagaac cgaatgaagg cgtccgactg gacgaaggtc tggtcaaaca attgactggt
    31021 ggtgataaag tcacggctcg gcacctttat ggaaaagaat tcgagttcga acctgaattc
    31081 aagttgtggc tggcaacgaa ccacaagccc atcatccgtg ggaccgatga cggcatctgg
    31141 cgacggttaa atttaattcc attcactgtc caaatcccgg atcacaaaaa ggataaaaat
    31201 ctgaaattta aattgcagac ggagttgcaa gggatcctta aatgggcaat cgatggttgc
    31261 ctgttgtggc agagagaggg cttggagaaa ccaaagtcgg ttgttgcagc gagtcaggat
    31321 tatcgaaacg aaatggatca aataggtaca tttatcgaaa cgtgttgcga aacaggtcca
    31381 ggactaaaaa tatcaggcgg agaattatac aaagtttatc gtgaatgggc atcagataat
    31441 ggcgagcata ctttcactaa cacgaagttt ggcagagaga tttcaaagaa atatagcaaa
    31501 gaaaaagctg gtggatttat ggtttataaa ggaatcacat taaaaccaag aaaatacgat
    31561 aacgttagag aactttttaa ataaatctgg agggttctgg agggttgaga acgaaaccct
    31621 ccaaccctta gaaacgttga tatatcagta tttatattat atttttcttt ctttctggag
    31681 ggttttaaaa aaataataaa gtattaaagt aaaaaataaa aagtaaatta tataaaaaag
    31741 aataggtttt tgggtctgac cctccaaact ctccaccaaa atcttcctaa atgctttgat
    31801 accaacgatt aaacagtctg gagggttaca ttgaaatcag aacatcaaat acaagacgag
    31861 atcagaattg ccttatcaaa acatggttgc acgatttttc gatccaatgc gggacaagtg
    31921 acaacgatcg aagggcggca attttacggg atgccaaaag ggttcccaga tctatgtgga
    31981 catcgtaatt ctgatgggaa agcaatttat atagaagtaa aaaatgaacg cggtaagttg
    32041 cgtgaagatc aaaaaaggtt tgctgaattt ttaaacggtc aacctgtact atacggtgtt
    32101 gcacgatcag tggaagaagc attgaaaatt gtggaggaat catcatgaca acagaagaag
    32161 tgattcaaat gcgtattcga agccttcagc gtgagattga cgatctggaa cggacaaagg
    32221 cagtgatggt caatgaaacg gcgaggaagg caatcgattt gcacatagag aatttaagaa
    32281 gggaaatcca tcgattggag gaatgagcgt ggataagaaa gcagcaatga aacgaatcat
    32341 cgaactgaca cattctgaga attggcaaga agacaaagaa atagttgcag aagtccaaaa
    32401 gctcggtaaa tcaatgtgga gtgaaaaaac caaacggaga acaccgagaa aaattgcaat
    32461 ctggcatggt gaccgaattc tagtaacagg tactgctgaa cagttatctg aaattactgg
    32521 attaagcaaa aacattatct gggatagagc taaacgcgag aacgttgatt cgaaaggtcg
    32581 tcaatttaaa cattgggaga agaaataatg gaggaactaa tcacaaaagt agagcagtgg
    32641 gcaaaagata agggattgga tcaagctgat tccagcaagc aaatgttaaa aacgatcgaa
    32701 gagattgggg aagttgccgc ttctctagct agaaaagatg aatatggttt aagagacgga
    32761 attggagacg tagtagtaac cttgattatt ttagctatgc aaaatgatat ggatttgtac
    32821 gagtgtctga accaagcata caacgaaatc aaaggacgca caggaaaaat ggtagatggt
    32881 gtattcgtga agtcgagtga tttggaggac agcgatgaat aaaaaagtat taattgataa
    32941 acaagcattg attgatgaat taatgaaaat acctggtgtg ggatctaata gtgacgcttt
    33001 agaaacgatt aaacgtttcc catcgtatga accgcagaag ccagttgtgc ctaagtttgt
    33061 ggcggaacta ctcgactatt atcgtcagtc aacagatgtt gatttattag ctttattgat
    33121 aactttccat gattggtatt atcgcaaaac taaagacggt gagcatgaag aagcaattga
    33181 ttggctagtt gatcatcctg aaatttatat gcgtgcatgg ctgtttggct acgagctcga
    33241 gaaagagccg ttgtattgcg taataatcaa tggtagatat cttgtcaaag tattcagcaa
    33301 tacaaacgtt gtcatattag ttccagcaga tgaattttca cagtacgtta ctcaaaaatt
    33361 tcaattaact gaaaaacaaa tcaaagcaat tgatgaaaga tactggccgt ttgctgtgcc
    33421 agtggatgag gtggtagaag gatgagcgac ccatacatta ctaaattaga aaagttgtcc
    33481 gaagattttg agaagaataa aaataaacta actaaagata aacttataga actggttagg
    33541 ttggttatca gaggagctat tacaagagaa aaggcgcagc aaaacataga aatttttgct
    33601 cgtagtgtcc gagatggatt agagaagaaa gttaaacaaa atgaacaaca gacaccgcag
    33661 aataacaaaa ctaagaaaac gggaactgaa tgtactaaag acaaagtttg aaaaagaata
    33721 tggaatttca gcagaagaaa catataaagt ggcaagtcag tgtgttgctg atgcgagcgg
    33781 cgctattcgt aagtttggaa tttcgatatt aagtgatgat cgtaaatggg aggaaaaaga
    33841 atgaaactaa aagacggatt ttactccagc agtcatggta tcggcggttt aatgctagat
    33901 atgccgacaa agaaccctaa aacacgtgag aaaccaaaat tcaaagtcgg tgacatggtt
    33961 cgctgtgaag cagaaggatt catctatcca ttttgtggat atgtagaaca tctctataat
    34021 cactcagcaa tcattcgtat tgaaaacacg atggaatgtg acaagtggtt agcgaaaagc
    34081 aaagagaatt tagcagtagc gagattggtg gatatggagg ttatataaac agcgaaaaaa
    34141 tgtacttcta agagaaaaat ttatttttta taaaaatagg aaagtatcat tatgaaaaag
    34201 accacagaga taaataaacc taccacataa aaaatgaata tttcttgcga ttcaatatag
    34261 gtatatttaa caatttgtac aataaaaaat actataggta aaaaagataa catgaggagc
    34321 ttatttgttc tagagtgttt gacaaagtaa aaaaatgcga cgataccaat acaaacggga
    34381 atccaaaata acagcatgct ccacatagtc actcaaccat ctcctatacg ctttattatc
    34441 aatagataat atcatttttc gtaataaaga taaaaaaata agtaaggctt ctcattaaaa
    34501 tataaaaaag acagccgacc actgactgcc tatataagag tattgaaata aaaagctgct
    34561 gatataataa attccacaag tttattatat cacataaagg agcggtttga cttgatgcaa
    34621 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    34681 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    34741 acggatatgc cgaaggcgcc gaagcacggc aataaggcag aagacgcaat cattcagatg
    34801 atggatatag aagcggagag agacgcgatt ctagcagcct tgatggctct tagtctgatt
    34861 agtcgtcaga tactctacta tagcttttgt gtgccagata gcttctcaaa ctacagaatt
    34921 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata
    34981 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataatt tggcggcttt
    35041 ttggcggaat gatggcggtt tttagctatt taccagtgat attatggtag tgtcgaaaga
    35101 ttagtgatag gtctaagaca aaataataat aaaaggaaca tcgttttatt attgtttcac
    35161 aattaagctt cgatagacag caacggaaat attaagaata aggatgtgaa ttttaactcc
    35221 ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttgttttttt aattattcac
    35281 acgataacta aaggtgagcg aagagaaatg attccattaa taatttcaat ttttgcgctt
    35341 tgtcttaatg tctatatgat tggatttaaa aatgggcaaa ataaaagata gtagtaacta
    35401 agaaagaaat ttttatattg tcactgtggc ggaaagggta gacgctaagc atgtgtgcta
    35461 ggtcaatgct tcggcaacta tgcaatgttc gattcattgc cagtgacttt ggcaaccgag
    35521 gcatcggcgg tttaaaaata taggggtgcg caatttcgta cgcgttttgt gcatcgtgta
    35581 ggttgctatt acatattaga tcactcgttg agtggtcttt ttattttaca taaaggaggt
    35641 aacaacgatg tatagaccac aatacttaga acagaagtat gaagtaatca ctgtgcaaaa
    35701 cggtaacggt gagatagtac gaaagtatag aagaccaata aagagcgata catataaacg
    35761 aaaggaaaac aatgaagtta tttcttttcg cagaaggaga aaagccaaat gagaaactac
    35821 tggtatgtat cgctaactaa tgaatatcct cgaaccattg atgattgttc agtgcgtgtt
    35881 gtgcgttctg tacaaatcaa agggaagtac tctattgtcg aaatgctaag agaagctaca
    35941 ccaaacgaag tggataaatg caagctgata tattgcggtc atggctattg gaaagacgag
    36001 tatatccaac aaaacattga gaggtggata gatagatgag ttacctcgaa catttgaaac
    36061 gttgctacat gcattctaag aataaacttc ctgacagcta cactgtagat gatgttgcta
    36121 tacatgtatt gaagactgag agccatagca gtcctgatgg tagcagtaag gagcagacgc
    36181 tcgcgtggtt caagttcttt aagtggatta aggaggaaga gtaatgcaca tgcacatagg
    36241 agaaaagata tataaattga gacggcttaa taacgaaaca atgaacgact tagttaaaca
    36301 aactggagtg tcaaaatcat ggttatctga tattgagtct ggaaaaaagc aacgcgttga
    36361 ttttaataag atatgtgcaa tagccaaaca ttataaagtt agcttagaat actttcggga
    36421 tgattatttg gatagtgatg aacacgagga tattattcct ttgacaggcg aaacaagagt
    36481 taaatcagtg aaatcgcttg aacaaagcct aaagtatctg aataacaaac aagaacctaa
    36541 ccttctagag atcaaactaa aagatactga ctcagtacca gaggtttggt acaagggtga
    36601 gaggttggat gaattgccta aaggattagt agatgtctcg tatcattgga agactgatga
    36661 ttttactaat gatgatagag gagcgaacga catcacgatt caatactttt ctagctttaa
    36721 tgataagtat ccagataaga aaacaatcgg acacaagaga gatatgtaaa tgaaagaagt
    36781 tagacctaga gacgaaatag acaaactata caagaccaaa cgatggcgag acctaaggca
    36841 agtagtaata gctagggact tcggcatgtg ccaagagtgc aagcgtcgag ggcggaacac
    36901 aaggggcacg atcatccatc acatagtcga ggcgagggaa gacctgtcac tgttctggtc
    36961 cgtagataac cttgaatgta tctgtgtagc ttgtcacaac agagagcatc cagagagatc
    37021 aggcgggaag aagaaaccaa aacctaaatc acatatcgtt aaaatgtatt caactcctga
    37081 aagataagtt tgcagcgaaa tgaaggtagc ccccctactc taaaagatta aagagtaagg
    37141 cttgagaaga acggtgctgt ccttccttcg taaaaagacc gcttttcaag ttttttggag
    37201 aaaaaggaaa aagccgacca atttaagccg gctttggacg aagctatttc ttagtccatt
    37261 tgtttccttt ttgagaagta gggggtaatc ggtcgcctgg atcaattttt acttcttgtc
    37321 cgccttggac atttccacca cgaggaccca cttctttgta ggttcctttt ggtttgttgt
    37381 cttcgccggg tttatagagt tctcccatag gaatccctcc ttaacaaatt tcggcacagc
    37441 actgccgata acctaattat aaggattgtg ataacgattt caatctatct tttgaaagaa
    37501 ggtgatatta tgccgcaacc agcgaagagt gcaaaattac aattattaaa cggaaaccca
    37561 aataagaaga ataccgaaga actccgcaag cgagcggccg cagaagacaa attaaaaatg
    37621 gctactgaca aaatcaaacc gccgttatgg ctagattcgc taggaaagga tacctttgag
    37681 tttatcgccg atgaattgct gtctgtggat ttaatcagta atccggacgt ccatacaatg
    37741 gctctatact ccaattggta ttcgcaatac gtttctttag aaaaacagct tcgaaaacta
    37801 caacgagagt acaagttgaa ctatgcgctt gcgaaaaagg aggcagaggc gagaggtgag
    37861 ccgtttaatg aacctaatga attaattggt aatccgctct ctcggcagat ggatacagcg
    37921 tctcggaatc tccgttcttt tggcgctgat ttaggactat caccagcagc cagagctaag
    37981 ttagctatta agatggctga tgatggtggt gatgacgatg acgacttcta atattttgga
    38041 tatgtcttac acagaacgtg tggactattg gcaaagctat cttgaggagc aagcttcttg
    38101 gggtggcttt ttaaaatgtc catatccgga attgttaact acttggtatg cggaacgatt
    38161 aatcgatgga agcataccag ccagcaaaga aaatattcaa gctgctaaac ggcatatgcg
    38221 tgatttgcag cgccaaggaa cagatgattt tccttggatc tttgacgaag aaaaaggtca
    38281 ccggcctatt agatatatcg aaaaaaaatg taaaccaact gaaggcgact ttggttcgtt
    38341 tgttttgcaa ccttggcagc atttcataat tggatccatg tacggatggg tacatcgtga
    38401 tacaggagag cgtcgcttcc gcgaggctct tatttttgtt ggacgtaaaa acggtaagtt
    38461 ttgccgttta aaaatcgggc aaaatcggtg aaaaccttta tttttggctt tcttttagta
    38521 ataattcgat agctttatca agcaatttag acataggaac catagtttct tccgacattt
    38581 tttttaatcc ttcgtataaa tctttatcaa ttgcgttaga aattctaatt ctgtttttta
    38641 gtccgtattt gtccatgtgt gacactcctt tttattttac tataacatcc gattccaccg
    38701 cttgcaacta ccatcaattg atggtagtat ataaaagagg tgagtagatt gaaaaaatac
    38761 attatttaca aacacttaaa taaaataaat ggcaaaattt atatcggtgt cacaaatgat
    38821 attggcagaa ggtggaggag cggtgggatt gaatataaac caccaaagaa cgaaacccaa
    38881 caccacagaa gtttttggaa tgcaatacaa aaatacgggt gggaaaattt tgatcatttg
    38941 attattgaag aagacttaac gatgaaagaa gcttttgaga aagaaaagtt ttatattgaa
    39001 ctttatgact caactaataa gaaaaaaggt tataacatcg caaaaggcgg aaatggcgga
    39061 ataatatata aagttcaccc caaaggaatg ctagggaaaa aacaatctaa agaattttct
    39121 tcaaatcaca gtaaatgggc aaagaaccat aagaataatt gtatgacaaa cggtgatgtt
    39181 gtatggggtg ttactcacga acacccaaaa ggcatgttgg gaaagcacca cagcaaagaa
    39241 tcaattatga aaaagaaagc atatagtggt gaaaatgctg taacctcaaa accaattgtc
    39301 gcaattgaac gggatggaac taaaagagaa tttcacagcg ctaagctttg catggcttac
    39361 tattcgatta gtacatgtgt tttttaccga cttttaaaag atggcgcccc ttatgtgata
    39421 aatcctaaag cgaactacag aaataaagaa aaaatattag ctatcgaagg aattatgttc
    39481 aaacataaag aagataccga ggtaagttag tagattgcga aaggttacta actaccgtaa
    39541 cgcgtaggag ttgaataaat ataatgctcc caagagtgtc cgacagcaga taatttgctg
    39601 aaaatgtacg ctaaactggg ccagaactga ctgaccgatg aaaatgaggg tgacctccag
    39661 agtgcgagat aaaaagctcg cagataataa caatcgaaaa catcactaat ttcaggcctt
    39721 tccacataca tggtcgctta tgatgatgaa caaggcgcca acgtttacgt attggcaaat
    39781 gctcgtgatc aagcaagctt gttgtttgat aaggccgcag aaatggtcaa acaatcgccg
    39841 gcgctcttta agaaatttgg taagcctaaa cgatcaagta ttaattatgc tcccgccttt
    39901 tctaaaatgg aaccacgcgc ctcagatagc cggaaattgg atgggctaaa cactcacttt
    39961 ggtatttttg acgagatcca cgagtttacg aattacaatc tgatcaacgt tatcaagaaa
    40021 tcaagaggaa ccagaaaaca gcctctgata gtttatatca caactgctgg atatgtatta
    40081 gatggtccgt tgatgtctta ttttgagcaa ggtgtggact gtttggaaca tttggaagat
    40141 gacatcgatg aacggacttt ctattatctg gcaaaacttg acagtgcgga agaggctgat
    40201 gacccaagat tatggatcaa agccaatccg aatatttgtc taatgaattt tgttggcatg
    40261 ctagatgact atgttaagga taaaaaagat ccaaaagaat atgctgactg gattaccaag
    40321 caatttaact tgttttccga tatcgatgag ctgtcatttg tcgatatgcc taccattaaa
    40381 cgaaacaata aaaccatcga tattgaaacg ctcaaaggta agaagtgtgt cggtggtttt
    40441 gacttgtccg aaacggaaga ctttaccgca gccgttttag aatttccgct tgaaacaggc
    40501 gaggtattca ttttgcaaca cacatggatc ccacaagcta gatttgatcg agataacaat
    40561 caagagcgta tcaaagcgtg ggagaaggtg ggagatctaa cgattattcc tggtgattac
    40621 gtcaattatg aatacgtctt aaattggttt gtagaaaatt cgaaaatcta tgacattgta
    40681 aaaatcaatt atgacaaggc caaggcgcta cgattaaata aagaactaga aaatgcagga
    40741 tttgaaacca acgagattcg gcaagggttt ctatcattag gtgggccaat gcaaaacttc
    40801 aaggaaatgc tattggacgg taaggtgatt ttcaacaatt ccaagcttta ccgatggtat
    40861 ctatccaacg tcaagctggt gatggatcgc aactcaaact ggatgccgtc taagcagtcc
    40921 aagagtagaa aaatagatgg ttttgcagca agtttgaaca gccacgccga agtgttgaat
    40981 atgttggtta atcctgtcgg aaccgggaaa gtaacctatt actcgatttc cgatttaatg
    41041 aatatgtaag aaaggtgtgg aggaatgagt attttagatc gtttgcgttc ttttggccga
    41101 gcgaagccga aagcgagcaa acaagagtat tttttgaatg acccgggatt gataccgtat
    41161 ttagtcggaa aagatgaaat atcagaaggg attttttccg taattagccg tgtatcgaac
    41221 gtttttgcgt ctctccctct caaaatgata gatgtggagt ttggccaacc ggacgactgt
    41281 cctgcataca acttgttgag cgaaggccct cgatatttta caaagtttga ttttttccgg
    41341 gacgtggaag ttttgagaaa ctaccaaggg aatgcgtatg tgcagatttt ccgaaatatc
    41401 aatggagaag tagcagatat ggcgttagta aaacctggtg cttgccatcc agtgattgat
    41461 atggatagcg gggagcttta ctaccaagta actgcgactg acaaaggcag ttacaagcaa
    41521 gttatctatg tacattacat ggaaatgctt cactttaaac aaccgaggtt tggcggcttg
    41581 gaaggtacag accccacaaa agtattaacg aataccctcg gatatgatcg agaagtccga
    41641 aaaatctctt taagtcagct taaaggaagt aatgaagggc taaaagttaa gtttgctagc
    41701 aatatggatg aagaagctaa aaaagctaca gttaaaaaca ttgctgattt ttatcgacaa
    41761 aacggtggac tacttgtgga agaaaacggt gtagaaatcg aacgtttaca acgagagctg
    41821 gtagacagca agcttttaga tactgataaa gtatctcgct ccagaatcgc tatggtctac
    41881 aatgtgccgg agcatttcat cgggaataac cagtcgagtt actcctctca ggaacagctc
    41941 aatatggaat ttttgacgta caatctagta ccgaccgtta atcaatatga agcggaacta
    42001 aataagaaaa cactatcgag aaccgaaaaa gcaaaaggtt atcgatataa gtttaatgtc
    42061 gcaagtttgc taagagctga cactcaggca aggggacaat tctatcaaat catgcgacga
    42121 ggcggagcat actcagcgaa tgatgtccga agatttgagg acttgcaacc gataaataaa
    42181 accggtatgg atgattacca tatttccgga gacctatatc caatcgatat ggatccaaca
    42241 ttaagaaaaa caacctcgtc taaaagcgta gccgaaaacg gttaggtttt tttagtttgc
    42301 accgaaggga ggtggaaaaa tgaaaaaagt gacgttaagc ggcgatgtcg tggataacga
    42361 taccgcgtgg ctttatgact ggtttgggat caattgtatc tcaccaggga aaatttctgc
    42421 cgctcttaca gaagcagcgg gggatgaagt agaacttgat atctcatcga acggtgggga
    42481 tgtcctagcg gcaagcgaaa tatataccgc tatccgtgcc tatccgggaa aggtatccgg
    42541 aaatgttgtg agtattgcag caagcgctgc aagtgtaatc gcttgcgctt gcgaaccgct
    42601 tagaatctca cctacggcac acatcatgat tcataacgca tgggtgacca ctagtggcaa
    42661 cgctgaggaa ttaaaagcca atgcagaaat gttaagcagt gtggatgagt ctattgttaa
    42721 tgcttacgag atcaaaacag gactcgatcg gaaaaaactt gctgatttaa tggcgaaaga
    42781 tacttggtta aatgctcaaa cagcagtagc agaagggttt gcagatgaaa ttatgtttgc
    42841 agaagcacca gtaacggtac tcaatgcctc tcaaccggtt attccaaaaa acgcagtaac
    42901 taagttgaaa aatttaatac tcaaagcgga agcaccgcaa gaagaaacac tcttacagaa
    42961 aaaactaaaa gccttaaatg gagggaaaaa cgaatgaatt tagaacaatt aaaaaatgcg
    43021 tgggtcgagg cgggaagtaa agtctctgac ttaaatgcac aactcaatgc agcattggtt
    43081 gacgatgaaa aaacagaaga agatgtagta agtttgcaag cacaagtaaa agcagcacgg
    43141 gctaaacggg acggattgaa agagcaagtg gcaaatatgg aagccgaaca agtcttaaac
    43201 gtcaaaaaag aaccattaga taaaaaagat gaaaacttga aaaacaagtt tatcaaagac
    43261 tttaaagcga tggtcaatgg tgatcctgct attatggcta ctttgacatc taatacggat
    43321 gaatctggta atgctatcgg attgactatt cctgtagatg tgcaaacgac tattcatact
    43381 ttggttcgtc ggtttgactc tttgcaagaa tacgtaaacg ttgaaaaagt gaccactacc
    43441 agcggttctc gcgtttatga aaaatggtct gatattacac cgttgactgc tttggatact
    43501 gaagacggtg aaatcccagc aaatgatgat cctgcacttt acttgatcaa atacttgatc
    43561 aaacgctatg caggtatttc cacagtaact aacagcttgc taaaagatac tgccgaaaac
    43621 attttggcat ggttgtctaa atggatcgcg aaaaaagtag ttgttactcg caatacaaaa
    43681 atcttggcag ctattgatgg aatcaaagcg gcacaaaaga aagatgttac agatgttgat
    43741 ggaattaaag atatcgtaaa cgtccaactt gatccagcta tcgaagctac atctatgttt
    43801 attacaaacc aagatggctt caatgtttta gataaagtga aacgtgctga tggatcttac
    43861 ttgttacaaa aagacgtagc ttctgcaact ggatatacct tcttaggtaa accaatcaag
    43921 aaaattgctt ctcgtttctt gccaaacaaa ggtacgcaag ctactcctaa atatccactg
    43981 tacattggtg atctgaaaga agccgttaca ttgtatgatc gcgaaaacat gagcttgctg
    44041 acaacgaata ttggtggtgg agcttttgaa acagacacca ctaaagtacg cgtcatcgat
    44101 cgcttcgatg tgcaactagt tgatgatgaa gcggttgttt tggctacttt tacaactatt
    44161 gcgaacgaga caccggcgaa agtttaagga gctgattcct tatgattctt gatcctaaaa
    44221 tggatttagg cgaaatcaaa aacgcactaa agattgatac cgatgatgac gatgtggaag
    44281 taagccgtgc ggcacaagct gcaattgcat acattagagg ggctatcggg aatgataagc
    44341 cctcttttta taagcaagaa aatgaaacgg ttgatctgat taatttagct atcctgcaat
    44401 tagcagatca ttattacaac gctcgatcag caaccgtgag tgggaacttg cgagagtacg
    44461 atttaggttt tacaagccta atcttgcaac tcaaagcaag ttatttgctt tttgtggagg
    44521 aggagtagcg tatgcccctt atccaaacag gaaatttaaa tcaacgcatc aagtttgtcc
    44581 gagatacgac ggttaaggat gaggacgggc aagttgtccc gacttctaca accattctta
    44641 cttgctgggc aagtgtgcag acacaacgcc tgaacgatat taagacgtcg attggtacgg
    44701 ctttggaagg aacactgacg ttcattatcc gctaccaaca aaaatcagag ctgaccaatg
    44761 atatgaaagt gcgttggaat ggaaaaacgt ttgaaatcat tacgattacg aaaggcgagt
    44821 ttgcgaagga cttcacgaca atcattgcaa aagaggtttc aaaatgagtg tagaagtcga
    44881 tgcaaccgaa gtgtacaaag cgcttaggga agtaaaagca aacgttcaac gagtggaaag
    44941 cccagcactt agaaaagctg gggagtacgc tcaagaagag ttacgacaaa acacacctta
    45001 ctgggatgga acgaagtcaa acggtaaacg tggttcgtat atgcaagaac atgctaagaa
    45061 ccatgtggtt acaagctcga taaaaaacgg attggtagaa gtcggctatg acaaagatgt
    45121 ttcttggcgg atgcacttta tcgagttcgg aacaatcaaa caacgtccaa aaggtttcgt
    45181 acaaaaaaca caaaagcaaa tcgaaaaaca agtaacacaa atcattgctg acgaagtaaa
    45241 aaggaggcta ggactttgaa aacggcagta tcacaagtct attcaattct gaatagcaat
    45301 gaaaaaacaa agaacattga tttttacacc aatagtgttc cggaatcagc tcaaacagta
    45361 cctagccttc cagttggcag aattacagag atatccggca actatgaaga ttttgcaagc
    45421 aacaatcctt tgaccattca atttaacgta caggtagatg tatgggtgtc aaccatgaaa
    45481 gaggttgatg ccttttattt tgcccttgat gaggttatga gggggaatgg ttggcaatgc
    45541 gcatacacgg aacaaacaga tgacgaggac ttggaaggtg caaagcggat tatcaaacga
    45601 tatgtagcaa atatttcact aaattaaaag gagagaaaat agatggcaac agtaggattc
    45661 gagagcgtca tttttggcgt aaaaacaggt gcaggcggca ctctaaaaga attagtagca
    45721 gataagtcga aaggcggagc gatcgaagct aaaattactg gattaggcgc aacttctaac
    45781 acaacatacg cttcaaacgt accgttcttc attgcaagta aaggggtttc gtcgccaaaa
    45841 gttacgcttg acgtggcaga cttaatggat aacggcattt acagcgaaat cattggtgct
    45901 aaaaccgtgg atggtgcaaa tgtaattggt tcagaaactg aagcgcctta cgtgtcggta
    45961 gtcatggtta cagcgaacaa agaaggaaaa cgcttgttca tgggattgac aaaaggaaaa
    46021 ttcagtcatc cagatatcga catgaaaaca gctgaagaca aaggggtaga attgcaaacc
    46081 gattccatcg aaggggaatt catttctgat gaacgtggct atgtatactt aacagccgta
    46141 gaatcagaag gaatgacctt acaaaaattc aaggacttgg taaataacaa agagggggag
    46201 tagttaaccc tgcatctaca ccaacgacag atacagggac accaaaagaa ccagaaccaa
    46261 aaattgatac acaaggttag ccatttttgg ctagccttat tttttgtaaa aacaaggagg
    46321 aaaacaaatg attgaattgc aattgaaact tgacggaaag aaaaaaacat tcaaacaaca
    46381 agatatttcc gcacgtgcaa tgcgtgagtg tatcaaattt tacgagaaag cggaaaaagc
    46441 agacctaact gatttagaag cgattgattc aatgattgca attacagcag atattttcca
    46501 agatccagca gttacatttg atgctatttt agacggtttg actgcgagcg agttagtacc
    46561 ggcattagaa agtgtttttg aacaaataaa tgaactggga aacaatgaaa aaaagcagac
    46621 ggcgagcaaa aagaaataag tttttctgaa gctaggaaag caatggatca aatctacaaa
    46681 gatttaatcg aagcaggttg gacgatgaga gatgtggacg aagccgacta tcattatttg
    46741 ttacaccttt ttggagaagt ggagagtggc gaagaatatg tagatggtgc tgatttcatc
    46801 aaacaatttt tatcggctga agacttagta aaacttgagg aaggaggtaa ataatggcag
    46861 gaaaaggaca accggcagga aatatcaagc tagggattag tttagatagc actagttttg
    46921 gtaacacgct ggacgaaatc aatgcgaaag tcaaacaagc tgagtcgaat atgcgtgcca
    46981 atctaaaggc ttatgattca gcaggacgtt catacgaagc acttagtcaa aagacgaaag
    47041 acttgtctac ggttatggaa gggcaaaacg ccaaagtaag agaattaaca aagcgccgtg
    47101 atgaagcgat tagcaagtat ggcgaggaat cgaaacaagt tgctaacctt aacacacaga
    47161 taaacaatgc taccgcaaaa tataatgctt acagtcgcca gttgaacgac acaaaaaaag
    47221 aattggtgta ttccaaaaca gccgtcaatg atttatctaa tgaaatcaaa gaaaatgaac
    47281 gacaaatgaa cgccgaagta aaagcgttga aagccgctgg tgatgaatct ggtgcttttg
    47341 aagcaaaaca aaaagggcta gccaaacaaa cggaattatc cgagaaagct atcgaagaac
    47401 agcgcaaagt tgtgaaactg atggctgatg agtttggcga ttcagcaaat gaaaccgaag
    47461 atgcaaaaag ggcattagaa aagttagaac gacaaagcca aatatctagc aggcaattag
    47521 aagcactcaa aagctccagc gatcaatcag gaaaagaaat agaagatttt ggcgacaagt
    47581 ccacaaggtc agctaggaaa ctggacggac taaaagacaa attaagctcg ctaaaaagcg
    47641 cattttcgtt tggtgcagtt gctggattag cgcataacgc tattagcagt gtagtaagtg
    47701 gcgtgcaagg tttggttggc gaagcagtaa acgcatcgga ttcattgatg aagttttcca
    47761 aaaccatgga gtttgctaac tttgggaagt cacagataga aagctcgaaa aaagaaatga
    47821 aagactacgc cgataagacg gtttatggtt tagaagaaat tctgaacaca accgcacaat
    47881 tggcatctaa tgggattcct aactatacag aactaaccaa ggcggcaggt aacttgaatg
    47941 ccgttgcagg cggttctagt gatacattca aatccgttgc catgatgcta acgcagacgg
    48001 caggagctgg gaaactaaca actgaaaact ggaatcaatt agcagatgcg ataccgggtg
    48061 cttcaggatt gttacaagac gctatgttga aaaacggagc ttatacagga aacttccgtg
    48121 atgcaatggc gcaaggtcaa atcacttccg acgagttcaa ccaagcaatt gtacagttag
    48181 gtatgaatga cggagcagtt aaggcagcca cttctacaga cacattgagc ggttcttggg
    48241 agcagatgaa atccactgta ataaatgggc tacaaagtat tatagaaaaa ataggcgttg
    48301 aaaatatcac tggtttcatc aacagagtaa caaaagggat tgaaaattct attcctaaaa
    48361 ttactcaatt tataggttgg ttgagagata ttggaacgtg gatcgttgaa aatagagagc
    48421 cactaacatg gattgtcgga atcataggcg gaattacatt agcagtaaaa gcattgaacg
    48481 tagcaagtat gttgctggca attactggcg gaacattggc agcccctttt gtggcgattg
    48541 gtgtagcatt aggcgcacta gcaggtgctt tggtgatagc ttatacaaaa tctgaaacat
    48601 ttagaaatat agtcaatgcg gcttttacag ctgtgaaaaa cgtagttatg agcgttatca
    48661 ataatttggt ggaatactac aaaatgttgt ggagcgtgtt gcagtggctt tgggaaaaaa
    48721 taaaagaatg ggcttcatgg attggcaata aattcattga aatgaagaac agcgttgtga
    48781 atacggttca gaacctatgg aatggcgtga aaaacttctt cagcaatggc gttggagata
    48841 cgtggaataa ggtagtcggt tgggtaaaaa acattttcaa caaagcaact gaattgaaga
    48901 acaacgtttc tgatgtaatc ggtaacctgt ggaatggtat caaagacaca ttccgtagag
    48961 gtatcgatac ggtattcaat tggttttcag aactaccaac gaagatgaag aatgccatta
    49021 ttggcggtaa aaacgccatt gttgatgcgt tcaaaagtat tttcaacgca gcacttaaag
    49081 cgataggtaa accagttaac gcaatcatcc atggagcttc atgggtacta gaaaaattgg
    49141 gcgcagaacc tctacaagaa tgggatgtac cacaatacgc tacaggtaca ccagcaggtg
    49201 ggcacccaat caatggtcca atgatggtta atgatggacg tggagcagaa acagttatca
    49261 caccagatgg tagagccttc attcctaaag gacgcaatgt tgttctaaac gcaccaaaag
    49321 gaacgcatgt cttgacagca gaagagaccg cgcaacttca aggttctaaa gctcctaagt
    49381 atcgttacaa aaaaggtact aacttctttg gtaacatgtg ggatagtgtg aaaaaggttg
    49441 ctggtaatgt aggtcacaca attaaaaacg tagtaggtga cgtgtgggac tttatttcag
    49501 acccgggagc gttagctaga aaagtacttg gggggttaga tgtattaggt ggattgacaa
    49561 agtatccatt agaagtaggt aaaggcatcc tatctaaagc aacaagtgca ctgactgaaa
    49621 aaatcactgg gttgttctca tctggtaact tagatacctc cgtaggaaca aatggcgtct
    49681 ataaatattt ggctgatgtt gctaagtctg tgatgaagaa attcccagga tttgtggcaa
    49741 ctagtgggta tagacctata gaccaggtga cccttattca catggtaaac gtaatgccat
    49801 tgatattgca ttaccaggcg tcacaggagg ctcacctaga tacacagaag cagcaaacta
    49861 cgcttttgac aaattcgctt ccaaaattgg ttacgtaatc actaatggga aagttcgtga
    49921 ccgttcagga caatcaggtc aaccagcaac tggtgcatgg gagccatggc ccgatggaga
    49981 tcactatgat catgtgcatt taaacggtgt gaaagaccca caaaacactc aaatttcagg
    50041 agatagcgtg ggaggcagtg gggtagaaag atggcgcaat gtagcaatta gagcgttgaa
    50101 aatgaccggt caatacagta ctgcaaactt aaatgcatta ctaaatcaaa tgcgtacaga
    50161 gtcaaatggt aatcctaatg cagttaacaa ttgggatatt aacgccaaaa atggaacacc
    50221 atcaaaaggg ttgctccaag tgattgaccc aacattcaga cagtatgcaa tgccaggatt
    50281 caacagcaat atttttgacc cactatctaa catcttagct tcaatcagat acgcactatc
    50341 aagatatggc tcactaacaa atgcctatcg tggagttggt tacgcaaacg gtggaattgt
    50401 aaaccaacat caaattgcgg aaatcgcaga aggaaacaag ccagaaatta ttattccgtt
    50461 agataaggct aaacgatcaa gagcgatgca attattgtct atcgcaatgg acaaactagg
    50521 agtttctcct aaaacttatg gaaatgcgac aactgtttcc agtgattacg aaatcctaga
    50581 agccattgaa aaacaggcgg caacaacgaa tcagctgtta tcgttattgc tggcattttt
    50641 caaaggaaat agccgaactg atagagattt agctttagat attcagaaga tcttggttag
    50701 gaggatgtag atgcgaactg tattactgag aaatagaata aacgaagaaa ttgatttgtc
    50761 cacagaagac tattttgcta ctgggttgag taatatgggg tttgaagtaa aaaaagaaca
    50821 tgtgggacag tgggggaatt tcagagaaag cagtgagagc gttgaaatat ctgaatttca
    50881 gtcatctgta atcatttctg tgcatgggtt tcgagaaaaa gaactgtaca attcacttgt
    50941 gcagttccta tcggaaggtc cgtttgaact ggaatttgct tttgacggcg aaacaatggt
    51001 aagaagatgc agtctaaaat ctttaagtaa gactgaaatc gatccgaaaa catcgttact
    51061 aacagacact ttagaactat atttcacatc aaactggtat tccgtaaaac gcgaaaaatt
    51121 aattcagcga ccgaatgtag tgaaaacacg tggtaaggtt tttccgtata aaagatctta
    51181 tatctacact caaaacttgt gggagaaaaa aggtgtattc aaattcaata ataattctgt
    51241 atacctaacg aatagcaagg aacggatgtc cccgctaaaa attcgtgtga ttgggaagtg
    51301 ttcaaatccg tattgggaag taatccaaaa ctcacaaatc atcgcaacgg atggatactt
    51361 catagatatg actgaaacac agactctaga agtatcaagc ctttttgaag atacaacggc
    51421 gattttaaaa gatattgcag gggtagaatc ttctgtctac caacaacagg attatacaaa
    51481 aactaatttt gttcaggccc caactggaga attcagcatt gtgtttcatg ttgggggggc
    51541 agacgtagag attgagttat acgaggagcg tgatctgttt tgattttagc agtcacgctt
    51601 tttcatcgtg atttaatgct ttataacgaa catttattct ctaacagttt tgaatttggt
    51661 gtggacgaga tcaacgaaga ggctagtagc tttacgatgg ataagtacgt tcctgtaaaa
    51721 acaggcgatt ttctactagc aaaatatatt cctagtggaa aatttgcata ttttggtgta
    51781 attacgtcgc aggaagacga aaaaatcagt tgtaaaagct tacttagttt agctgatagt
    51841 gagataccga ctgcacgtgt gtcaggagat aactacgaag agcatatccg gcggttgatt
    51901 gaatactatt tgctgaatga tccaacgaaa caactaaaag atattttaga cgtcaaagca
    51961 gaaagtgcga cctctcattc gtatcaagct accgatacga ataaacacaa gttaagtgcg
    52021 tatattctca atggtttcaa aaaatacaac gtaaaatggt atttcaaggg aattcaaaac
    52081 agaaaaattt atacaggcat acgtgctgta aatgaatcaa tttacattaa agacaattct
    52141 tctgaattta gcgattggga tgtgtttgtt caggcgccgg gcgctggaaa cgagaacaag
    52201 ctattaatcg ttgataaagc aatgaaagat atagagaaac cgataatact gtcaacatgg
    52261 tatttggacg aagaaaacaa tttgacacaa gatggatcga aagaaaatat cacgaaacca
    52321 actgtcaatt tggttaatat ctacgatcaa accgcagaag ataaagcatc ctacgaagat
    52381 gtggcaaaat cagagttgaa aggcaatacg tattcgcatg aaatcaaagt gaacgttgta
    52441 agaaatgcaa aaaatttgaa tgtcgaaacg attgaaacag ggatgtttgc cacgatttct
    52501 tataaaggaa agatatacaa gtcggttttg acagcttggc gaatatcaag tgataaggag
    52561 tttgtggaat tgactttcgg aaacataaga agtcgtttta tggattattt tgaagataat
    52621 gggggataaa aaatggttag caatgtggat ggatatcaat ttgaaaacgt gaaagtaagc
    52681 gcagaaaatg atgctagact ttatcacgtt ttatataatc ggaaaaatca ggttattgat
    52741 ggttacgatc agtctatgaa tttatcttca agcggattaa cagtaaaagt tgctgcagga
    52801 gcagcgatta ttcaaggtcg tatggtcgtt gttcgacaag aagaaagtat aacagttcca
    52861 gcaaactcaa gtggttatat agcattaaca gtggatttga cacaagaagt tatacctgga
    52921 tctattcttc cagaatcgga agaatatgaa tggactaata atcaagtcaa gctagaattt
    52981 ataacaaaag ttataaaagg taatttaaac aatggtgata aggtctataa tttaccacta
    53041 tgctcagtta attctactgg atcaactgtt tcaatctcaa agatatcgga tagttacgag
    53101 ctgactctct ctaaaggaga aattttgtgg agggggactg cgttaatgca tgatactcaa
    53161 actgtccaac cttcaaaaaa aatttggcag acagtttcag gttttttgtt agtatggctc
    53221 ccatacgaaa acgggcaagc aattgaggat agatatgtaa ctacgccttt ttataaggag
    53281 cgtgtaactt ttactaatgt tttaggagaa attgtttcag gatttgatga ctatcacaaa
    53341 aagtggttta gtaaacgaat aaactataat tcgaatacta atatatttac aggtgcagca
    53401 agcaatgcaa gtggagataa cgcaaatatg gtgcttaggt atatagtttc tttttagtta
    53461 ggaggtatgg aagagtggca aatttagaaa ttaaattatc tgcaaataag agacagcctt
    53521 atctacgtca ccgtgttgtt ggtagagttg gtgatggggg gctcacaaca atcaatgtac
    53581 aacttcttga ggaagatgaa attacacctt ttgtaattaa tctaaacggt actttgaaat
    53641 ttgtgggcga agtttcaaac ggtaactata ccgagggaga accagaaata atcgattcga
    53701 ctaatgggtt aattagttac acgttcacta agtcaaattt cagcacgagt catcaattca
    53761 aacaagcata ttttgaatat gtagatccta acggcaaaaa agtaactttt cagaacttca
    53821 tcatagacgt gttagaacga gtggatatta attcggagca agcgaaatac tatatttctt
    53881 cattggaaaa attacagagt gaaatgcaaa ccactttcaa tcagttcatt agtgataaac
    53941 aggtccaata cgatcaaatc tactcgaaat ataacgaatt agtaagattg ataaatgaat
    54001 cagataagca agtaaatgat cgtattgatc aaaccaatca gcaaatcggc gatctcggca
    54061 agctgaaaaa gatgtacagt aacagcatcg actttggggg ctatgattat tcggggaatc
    54121 cgaatttgtt aagtaagcta tcatacgact taattgaaaa tcaaaatact tcagctggaa
    54181 cactttctaa gggtgaaaac tcgtttaaat ataataagat atcagctgaa gtggaaggtg
    54241 gagtagagtt atattataaa cgaagaggta tagctaactg gttaccctct aataaaacgc
    54301 ttgtaatgac tgttaagctt agagctggag cggactatag tccagttgac ggaaaactta
    54361 tactgattag atataggtat gttgacagtg gaactggcaa gattgtttta gacttaccta
    54421 ttaacagtaa ttcgataact caggaatgga aagagtttag tattactgga actactccaa
    54481 catttagccc acaagcatac catccttgga tacaatttag ggctcaagat ggggtacttg
    54541 gggaaataga aatgagctat gacatcaaaa tcgaagaagg ctctacagct acaccatatc
    54601 agcctaactt acttgatgac tcttactggc taggtaaaac accgttgggt gagaatttga
    54661 taacgaatgc caagttccca attataacac gaagcaatcc tatttctggt tttgatattt
    54721 cagaagaatt aataataggt gaaacttata cggtatcttt aaaagggact aagccagcta
    54781 ctaaagaatt ccacttatac tacggagaag caaattacca gcagttgcag tatcaggcaa
    54841 ctttacttcc tgtggaagga ttaacagatg tgtggagtgc cacttttaca gccagaaata
    54901 ctagtgaaac gacaaattta cttagagtag cgttatggca aaaacctaat tctgcaacat
    54961 atgatactgt tcaaattgac tggctcaaga ttgaaaaagg cgacacacgc acaccaaata
    55021 tcagtcagtt taaatacttt ggtgaaggct tgaaagacag taacaatccg aacgactaca
    55081 gctgggatgt cacaccagag tatactgaaa aaagcttgaa taatacggtt agtctgactg
    55141 aaccgcaatc catcgaagga ttgaaaaatt tcgaagatgg tattcaatca aaaggaaaat
    55201 ctgtattgac atcagacgac aacaaatatg aagtcgtaac cttaacagtt acaaacggga
    55261 ataccggatc agcaaagctt tatcgtgaag gaaaaaccgt cactatttat ttttttgcgt
    55321 tgaatgggaa aagcagtggt ggaaatgatt caacgatact aacaattcca gaaggctatc
    55381 ggccaccaat tagttttgag caactggttg gctcgataga ccgttctact ttgaacagtg
    55441 ctcagttatc tattggtgca gatggagcca ttaaatggcg aagaaactca agttatggat
    55501 cggattatac ctttgcaatt acttacacga tttagaaagg cgtgaatcga tatgaaggca
    55561 gcatatagac caattgaacc ttacggattc gagcaaatca ttgtgaatga tgaagaacat
    55621 ttaccggaag aatgcacaga agtcgaacca ccgattccaa attggaaacc gaaattcaat
    55681 tactgggagg gaaataaatg aaaaacattt ggaaatatgg ccgtactggc ggagagtatg
    55741 caggaaaagt attggacgac atgcttgtat ccgttcctta cacagatcag ccaccgctcg
    55801 aaggggttcg ttcagatggc gaaccgctaa cgattgctga tcagatgttt gatcctaaat
    55861 tgaaccaatg gattatttta gcgaacgcgt tagatcacaa cgatttaaac aatctcaaag
    55921 cgatgtacga ggctcttgaa catgaaaacg gcaacctaaa acagctaaat gccaaactca
    55981 tgctaagcga tgtagcgatt aaacaggaaa atactgcatt gaaagaaaaa gctgacagtt
    56041 tagcacaaat caattcaaaa atgatgcttg cttcgttaca aaatagcaaa gacatttcag
    56101 aaattaaaga gcaactaaat ccagcttcaa agggaggtga gtagtatgtt tagttttagc
    56161 gatgtgaaaa tgatgtatga ttggggctgt tttactgacg atcaagttcg tctattcgtt
    56221 ccactatgta tcacagatga agaagcagat agaattatta gcaaagaaga gagcgcatct
    56281 taagtgatgc gtttttttgt tggaaagttg gtggaacatg aaagaagaag cgctccaaga
    56341 cgttgtggag agattagtaa gaattgaaac aaaattagac aactacgaat cacttagaga
    56401 aaaagctgat agtgcaaaag atttggcaga caaagcctac tcagtagcgc taaacaacgc
    56461 agaagatatc aaagaaatga aagccaataa taaatggtcg tggggttaca tgatcggttt
    56521 aggcattacg atcattggct atttcttgac taaattgtaa aggagttaag aagaaatgat
    56581 tttacccgat aagtattatc aagtcattaa atggacggtt ttaacagtat taccagctgc
    56641 atctgtttta gtagccacgt taggaaaagc gtatggatgg aatggaacag atatgacagt
    56701 actcactatc aatgcagtag caacattttt aggcgttatc actggtgtgt ctgcatataa
    56761 cttaaaaaaa taggaggaaa aaaatgaaga agaaaatcat tttatcattg agcctaataa
    56821 tggctctttt tttattgcca attaacgggt tcgcctatac gatcaacaat gaatttaatt
    56881 taggtgtaaa tgaaggtagc tc
//
LOCUS       DACEDD010000012        55081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEDD010000012 DACEDD010000000
VERSION     DACEDD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.012"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IWO45_000856"
     CDS             5..1087
                     /locus_tag="IWO45_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0332348.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IWO45_000857"
     CDS             complement(1305..2447)
                     /locus_tag="IWO45_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0332349.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IWO45_000858"
     CDS             complement(2452..2637)
                     /locus_tag="IWO45_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0332350.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IWO45_000859"
     CDS             complement(2991..3236)
                     /locus_tag="IWO45_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332351.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IWO45_000860"
     CDS             complement(3230..3631)
                     /locus_tag="IWO45_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0332352.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IWO45_000861"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IWO45_000861"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0332353.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="IWO45_000862"
     CDS             complement(5649..7415)
                     /locus_tag="IWO45_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0332354.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="IWO45_000863"
     CDS             complement(7428..8222)
                     /locus_tag="IWO45_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0332355.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="IWO45_000864"
     CDS             complement(8232..9608)
                     /locus_tag="IWO45_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0332356.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IWO45_000865"
     CDS             complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IWO45_000865"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0332357.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="IWO45_000866"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="IWO45_000866"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0332358.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="IWO45_000867"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="IWO45_000867"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0332359.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="IWO45_000868"
     CDS             complement(13042..14673)
                     /locus_tag="IWO45_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0332360.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="IWO45_000869"
     CDS             14977..15648
                     /locus_tag="IWO45_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0332361.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16373..16708)
                     /locus_tag="IWO45_000870"
                     /pseudo
     CDS             complement(16373..16708)
                     /locus_tag="IWO45_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16805..17257)
                     /locus_tag="IWO45_000871"
     CDS             complement(16805..17257)
                     /locus_tag="IWO45_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332362.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17264..17812)
                     /locus_tag="IWO45_000872"
     CDS             complement(17264..17812)
                     /locus_tag="IWO45_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0332363.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17857..18189)
                     /locus_tag="IWO45_000873"
     CDS             complement(17857..18189)
                     /locus_tag="IWO45_000873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332364.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18203..18433)
                     /locus_tag="IWO45_000874"
     CDS             complement(18203..18433)
                     /locus_tag="IWO45_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332365.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18655..19197)
                     /locus_tag="IWO45_000875"
     CDS             complement(18655..19197)
                     /locus_tag="IWO45_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332366.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19218..20309)
                     /locus_tag="IWO45_000876"
     CDS             complement(19218..20309)
                     /locus_tag="IWO45_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0332367.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20317..22527)
                     /locus_tag="IWO45_000877"
     CDS             complement(20317..22527)
                     /locus_tag="IWO45_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332368.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22543..25071)
                     /locus_tag="IWO45_000878"
     CDS             complement(22543..25071)
                     /locus_tag="IWO45_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0332369.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25123..25533)
                     /locus_tag="IWO45_000879"
     CDS             complement(25123..25533)
                     /locus_tag="IWO45_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332370.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25546..25764)
                     /locus_tag="IWO45_000880"
     CDS             complement(25546..25764)
                     /locus_tag="IWO45_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332371.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25761..26753)
                     /locus_tag="IWO45_000881"
     CDS             complement(25761..26753)
                     /locus_tag="IWO45_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0332372.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26832..27743)
                     /locus_tag="IWO45_000882"
     CDS             complement(26832..27743)
                     /locus_tag="IWO45_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0332373.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27762..28106)
                     /locus_tag="IWO45_000883"
     CDS             complement(27762..28106)
                     /locus_tag="IWO45_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332374.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28126..28682)
                     /locus_tag="IWO45_000884"
                     /pseudo
     CDS             complement(28126..28682)
                     /locus_tag="IWO45_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28763..29263)
                     /locus_tag="IWO45_000885"
     CDS             complement(28763..29263)
                     /locus_tag="IWO45_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332375.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29264..29809)
                     /locus_tag="IWO45_000886"
     CDS             complement(29264..29809)
                     /locus_tag="IWO45_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332376.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29806..31062)
                     /locus_tag="IWO45_000887"
     CDS             complement(29806..31062)
                     /locus_tag="IWO45_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0332377.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31423..31734)
                     /locus_tag="IWO45_000888"
     CDS             complement(31423..31734)
                     /locus_tag="IWO45_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332378.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31757..33067)
                     /locus_tag="IWO45_000889"
     CDS             complement(31757..33067)
                     /locus_tag="IWO45_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0332379.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33681..34133)
                     /locus_tag="IWO45_000890"
     CDS             complement(33681..34133)
                     /locus_tag="IWO45_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332380.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34135..34500)
                     /locus_tag="IWO45_000891"
     CDS             complement(34135..34500)
                     /locus_tag="IWO45_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332381.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34599..38882)
                     /locus_tag="IWO45_000892"
     CDS             complement(34599..38882)
                     /locus_tag="IWO45_000892"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ0332382.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39029..39268)
                     /locus_tag="IWO45_000893"
     CDS             complement(39029..39268)
                     /locus_tag="IWO45_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332383.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39358..39630)
                     /locus_tag="IWO45_000894"
     CDS             complement(39358..39630)
                     /locus_tag="IWO45_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332384.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(39713..39964)
                     /locus_tag="IWO45_000895"
     CDS             complement(39713..39964)
                     /locus_tag="IWO45_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332385.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40960..41871)
                     /locus_tag="IWO45_000896"
     CDS             complement(40960..41871)
                     /locus_tag="IWO45_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332386.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(42067..43902)
                     /gene="lepA"
                     /locus_tag="IWO45_000897"
     CDS             complement(42067..43902)
                     /gene="lepA"
                     /locus_tag="IWO45_000897"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0332387.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            44049..45269
                     /locus_tag="IWO45_000898"
     CDS             44049..45269
                     /locus_tag="IWO45_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0332388.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            45289..45783
                     /locus_tag="IWO45_000899"
     CDS             45289..45783
                     /locus_tag="IWO45_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0332389.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            45776..46447
                     /locus_tag="IWO45_000900"
     CDS             45776..46447
                     /locus_tag="IWO45_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332390.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(46598..49207)
                     /gene="clpB"
                     /locus_tag="IWO45_000901"
     CDS             complement(46598..49207)
                     /gene="clpB"
                     /locus_tag="IWO45_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0332391.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(49458..50504)
                     /locus_tag="IWO45_000902"
     CDS             complement(49458..50504)
                     /locus_tag="IWO45_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0332392.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(50515..50787)
                     /locus_tag="IWO45_000903"
     CDS             complement(50515..50787)
                     /locus_tag="IWO45_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0332393.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(50822..51730)
                     /locus_tag="IWO45_000904"
     CDS             complement(50822..51730)
                     /locus_tag="IWO45_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0332394.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(51862..53094)
                     /gene="pepT"
                     /locus_tag="IWO45_000905"
     CDS             complement(51862..53094)
                     /gene="pepT"
                     /locus_tag="IWO45_000905"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0332395.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(53109..54230)
                     /locus_tag="IWO45_000906"
     CDS             complement(53109..54230)
                     /locus_tag="IWO45_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0332396.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(54227..54928)
                     /locus_tag="IWO45_000907"
     CDS             complement(54227..54928)
                     /locus_tag="IWO45_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0332397.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT        16607 a        11381 c         8243 g        18850 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
    16561 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
    16621 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
    16681 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
    16741 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
    16801 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
    16861 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
    16921 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
    16981 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
    17041 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
    17101 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
    17161 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
    17221 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
    17281 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
    17341 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
    17401 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
    17461 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
    17521 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
    17581 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
    17641 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
    17701 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
    17761 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
    17821 ctccataaaa aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc
    17881 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa
    17941 atgatcccat atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18001 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18061 atcttggatt tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata
    18121 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18181 ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18241 aaccagtaat tacactatct acaacgccat tccaatttac tctacatttt ccatttttca
    18301 gatttgtcgg tccatacgga tagccttttt gacattcaat gttcggatta taccagatag
    18361 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18421 atccaataca catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa
    18481 aatagaatag ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt
    18541 tctgtcctat aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt
    18601 ctgttttttt ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata
    18661 cgcactccgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt
    18721 caattgaagt aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata
    18781 ttgatccgga ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg
    18841 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct
    18901 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    18961 taagaaattt tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga
    19021 accaatttga ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc
    19081 gattgtttct tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca
    19141 accagtaaga cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt
    19201 tctagtcact gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg
    19261 tacgtgcgtt gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag
    19321 aaatgttgtc cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga
    19381 gacaatgcac cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt
    19441 gggttcatga ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc
    19501 gcaaacatgt gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc
    19561 gtaccaccat aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc
    19621 gcattgcctt tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa
    19681 tgcttgatcg caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct
    19741 cctcgataat acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga
    19801 atcaataaac aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc
    19861 gccaccataa agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa
    19921 ccaccttcag aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac
    19981 caggtaatcc caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat
    20041 tcatagccta attgagaaag aatcacactc ggtaataact gatactttac agctgcacca
    20101 actgctgctt gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc
    20161 ggtaatcctt cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca
    20221 tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa
    20281 agaagacaac ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt
    20341 ttggactgtt cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg
    20401 cagtgtttta ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga
    20461 aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg
    20521 ccccggttgt tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga
    20581 gtattggttc aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac
    20641 ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc
    20701 agaaggcaac ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct
    20761 ttgattattc ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct
    20821 tactgaattc atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt
    20881 tttcgatttg gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact
    20941 ataaggcaag gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc
    21001 ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc
    21061 agaagcgagt ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc
    21121 caaattttgt ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg
    21181 acttgctatg tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt
    21241 taataaatct ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt
    21301 tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa
    21361 aaatgccacc aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga
    21421 ctttaaggta ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa
    21481 aggaatcaca agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat
    21541 cactacaagt ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc
    21601 tttataaaca gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc
    21661 ttctcctgca gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc
    21721 cttggctaga aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt
    21781 cccataattt aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac
    21841 tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc
    21901 caattcatct agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc
    21961 atttaaccct ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca
    22021 aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt
    22081 ccctctaaat atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc
    22141 aataggatcg tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa
    22201 ctcgttcatt gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat
    22261 caatttactt aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc
    22321 cccaaataac attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc
    22381 aatatgaaat ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac
    22441 caatccacca acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt
    22501 cttttcctta tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt
    22561 atcctgttct tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat
    22621 ttcttcaaac aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca
    22681 acactgtcct ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg
    22741 taaatgattc aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa
    22801 attccctttt tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg
    22861 attcatctgg gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga
    22921 attattaaat acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt
    22981 tccaaacatt tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc
    23041 atctgtatat agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa
    23101 aatcgttgtt ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg
    23161 taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg
    23221 ttccatttgt tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt
    23281 ttgaactaaa acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc
    23341 taacggactt aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa
    23401 acaaacaatt ggatctaata caccataatt acggggattt gtagcatcta acgtgacata
    23461 actcattttt tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg
    23521 aatgagtttc gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac
    23581 attcataaac gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt
    23641 ttttccagtt actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat
    23701 ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt
    23761 tttttctcct cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga
    23821 ttccatacct aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt
    23881 tcggtgcttc caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa
    23941 ttctcgttga tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac
    24001 cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat
    24061 ccaatcgacg acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg
    24121 aacagttaat aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt
    24181 tttgaaccgt tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg
    24241 tggatagtga attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa
    24301 tagatcacaa tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact
    24361 actccctaac tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata
    24421 cgttccttga ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat
    24481 tcctcgaata tattgcagac gattgagata caacagctct ttttttacta agggacgaat
    24541 agaaaagcta taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa
    24601 agtttccaaa tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa
    24661 ccagattaga tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat
    24721 tcctaaaata aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc
    24781 ttttgcataa tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc
    24841 aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc
    24901 tgctaaaagc aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt
    24961 gttttttgaa attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt
    25021 taacaaacaa tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca
    25081 cctcccaata aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac
    25141 atacttattc acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata
    25201 atacacacaa tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa
    25261 atgcactttt tttccatcag gttttagttt tgccagtgct aacacacata aataggggag
    25321 tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga
    25381 acgtaaaaat aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc
    25441 aactggaaat ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata
    25501 aacaatcgaa taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa
    25561 tttttcaatg attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata
    25621 aactgcggca cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt
    25681 tcgcaatccc agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa
    25741 atctacaatt cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt
    25801 gttaattttt caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct
    25861 ttttgtaaaa gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt
    25921 tttttcgttt ctttataacg caatactttc atctctactt gatcaacttt agctgtttga
    25981 ttgagtcctt caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac
    26041 ttatctaaga aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt
    26101 tcgtataatg tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc
    26161 gtaaaataag gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg
    26221 agtacttgtg tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata
    26281 tcataagtta ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac
    26341 gaagcagcag ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt
    26401 gcataaaact ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat
    26461 gtattcatat acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt
    26521 aattctgacc gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac
    26581 gacacttgaa ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga
    26641 aataaaacca aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc
    26701 ggtactttct ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac
    26761 tccttctagt atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa
    26821 aaatagtcca ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca
    26881 aatccagcaa tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca
    26941 ccagtctttg gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt
    27001 ggaagtttct ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt
    27061 aatggaatac catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg
    27121 gttggtgttg gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt
    27181 ccttgagaag tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt
    27241 gctggaacaa taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc
    27301 ggttcttctt tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa
    27361 tcagtattct tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt
    27421 gaatccgttg ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt
    27481 gattcaggaa actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat
    27541 tgttcaggtt ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt
    27601 aatccttccc ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct
    27661 gcataaacca atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg
    27721 actgctaatg ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg
    27781 ttcatagacg ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct
    27841 aacccaacat atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt
    27901 ggatagtttg caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat
    27961 tcataacgat accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc
    28021 catcgttcca ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat
    28081 accaccaaaa ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat
    28141 catcgaacat tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa
    28201 acgttgttgc aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg
    28261 atcttctttt tcatagcatc ttgtagctct atgacgctca gcttgcatga cataaccaaa
    28321 ctctgcacca tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca
    28381 atcacaaaaa tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt
    28441 agaaagcaca ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt
    28501 ttcatctgtg aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc
    28561 ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa
    28621 tccatataat gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac
    28681 ataccatctt ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt
    28741 tctttccttt tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat
    28801 caatcggcac aagttcacga aaacttggag caattttgga tagaatcact tccccatctt
    28861 gtaaaataca atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga
    28921 taaaatagta gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat
    28981 catcatagaa ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt
    29041 ctggtaacaa tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat
    29101 ctaaacgtct gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca
    29161 gatagaataa ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat
    29221 cacgtgacca atacgcacca acataaatca taattttttt catttagaca atcacaccag
    29281 caatactaat gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta
    29341 attctatctc aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca
    29401 tttcccaagt gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg
    29461 ctgccacaca gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg
    29521 caggtattgg caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc
    29581 taaattccaa ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa
    29641 tttcttgcca gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag
    29701 gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag
    29761 cctttaaatc caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc
    29821 tcctgacgtt cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc
    29881 aatcccttct tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa
    29941 tgtcggagct acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg
    30001 ctttactgta aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac
    30061 aatccgtgca tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac
    30121 gccaatctct cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt
    30181 tgctttctca ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc
    30241 ttctaattct gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa
    30301 aaagagagga ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc
    30361 atcatctaaa aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc
    30421 tgctttttgt ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca
    30481 aagttcatct aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa
    30541 ctttccttga tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg
    30601 catgacttcc tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt
    30661 tacagttcgt tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa
    30721 aagatacaag cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg
    30781 aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg
    30841 aagatctatt ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc
    30901 ctctgggatt tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat
    30961 caattgcgca aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc
    31021 acttaacttc tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata
    31081 aaataacaaa atcgattttt tttaaccctt tagaaacacc gatatatcaa ctagtccccc
    31141 tcgttttttg ggaaaagtag tacaactgct gaccttagat aatcacttga tacaaaaggg
    31201 tttataaaac ttttttctat gtgttatgtt atttctgccc cccatattat atatcggggg
    31261 gctttacaca aagaggccgt gcgcaagcgc acgacctctt tgtgagctgc acacgctgct
    31321 agcgagtgcg cattataggc ccatgcttcg cagccttaat gcgcaccgcc aggcagagtg
    31381 cggctaccag actacgtcta gagccctccc gatagataat aactacaaaa ttaaatgatc
    31441 taattttgtt atttcactat atttttcagg agaaaaaacg cgataaattg acaaagttaa
    31501 atcctcttct aaatctactc gataatgata caatcgagtc aatcgttcta tatctttttt
    31561 cgttagcaat acttgattaa taacttttcc tgtaaaaaca cgttctttgc gcaaaaagcg
    31621 gtaatcagtt acttgttcaa tacgattcaa ccaatcataa aacgttctgg aagaaagaga
    31681 aggattaatc attcgtattg ctgtagctac tctataataa caactctctg ccatttaact
    31741 tcacctcctt agttgattac aaaatatgcg catcagaaaa acttgttcct tttggatctt
    31801 ttgctaaata catttggcgc tctagttgtt cttgtaacaa ctgattatca tctcccatcg
    31861 tttgatcttg actatcctct tttactgtaa aatcatagac aatcatttca gtaaatttct
    31921 caaattcttt gataaagtga taatctttgt aaacaaatgg cgcaaaaaac tctacaggta
    31981 catcaaatcc tggaaaagca tatccacgac caggttgatc tttgttgtaa aatggcttat
    32041 ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg agacaatcga ccaacagaaa
    32101 tacggaatgt taaattatcc cgaacaccac ttggaaaata ttctgcatct ggtcgttgca
    32161 ttgctaaaat aatgtacatc cctgcttgac gaccctttaa tactaatggc tgaatcaatt
    32221 tatcgactct atttctcgct tccatatcca acgaaccata aaaagctgcc cattcatcaa
    32281 aaaagataaa atgcggtggt aacccataat aagcataatt tttccctggt tcatatttag
    32341 gatctttcat ggttttcata taggcatacc gttgattcat ttcttttaat gcatttcgta
    32401 aacaaataat catttcttca tttattttgt aaaacacatg tcctttgaat acatcgacgt
    32461 cacctaatgc tgctaaatct gccgcttttc catcgcaaat atctactgtc ccaacactta
    32521 aacaagcttg aattaaagaa tacaataaat aggttttccc accaccgatt gcaccagcaa
    32581 tcaacatatt tggtgattga tcatataccc aatccaaagt tttcattaaa tgaatacgtc
    32641 cattttctgc tatacattga tcaataggaa tccgaccaga atctgaaagg aatacataat
    32701 tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg caaaaatact tcagataaca
    32761 aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc atctaaagga aaagaaacca
    32821 ctaattcttg tttcttttga taaaaataaa cttttggtaa tctcattttt tcccttgttt
    32881 gtttctcact aacttgtttc tttgtataaa caaaccgatt gtcaattaac attcgaacaa
    32941 gtaactgacg ccaataaaca gaggcaaaat atccttcttt tagtcgcgac caatgcatca
    33001 accctaaaat taacccacta accagcaaca aatacaacac aatacttatt ccaggaataa
    33061 gatacatttg ccaattcgaa aacgtactta aacgtggata aaatactttc caactaaacc
    33121 aacctaacag aatcaaaaat ggaaacaaaa agaaaccata atagcaaaac cataaataac
    33181 gattgaaata gcgaattcgc cgaccacgat acttgaaaaa atgagctttc aattgtcaca
    33241 ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt ttgacaaaaa gcaactaata
    33301 caggaatatc acatttagta aaaaaacata atcgaaaaag atttaatcga tttttataag
    33361 ggtgtgcaca catattacat attcgtacta tacgaatcca aatcacttcc tgatgatctt
    33421 gtttccactg gtacggtaat gaaaaacgaa caagatcacg taaaaacata cactgcccaa
    33481 taaccgcaca aatatatgca tttgtttgta caaaaatcgt tggattgtct ccaaaatact
    33541 caatcaattg ttggatattt tgataacaag cacaatctct caaagtattt gattggtttt
    33601 atctgaattc aaaatctccc cttccttcta tcttagataa aaagaatagg aagactcctt
    33661 cctattcttt caaaaaaacc ttattttttc tctttttcct tagaatcaac acttggttgt
    33721 ttttgatcta aacctttttc ttgtgattgt ttcatattag gattatttgt tggagcttct
    33781 actttctcta atcctttcgc ataaatcact aaatctgaat attcacgatt gccatcttgt
    33841 gctggaatat ataccacatg atattctgga tcaattaatt tcacaggatc acgtcgttta
    33901 aaaccagtca aatctacatc tgctggaaat ttaactcgtc gtgcaatctt tggttcatct
    33961 ggatttccaa aaactaattg tgctcgctgt gatcgaattt ctcccgtata atcgcctgtt
    34021 tcattatcaa atacacgatc agactcttct aatgcgccaa agcgtacggc tcctaaacaa
    34081 gtttcatcta atgcttctgt aatgtcataa ttatttggaa attttaatcc catcttacaa
    34141 aacctcccca atttttacac cagttaaaaa gacaccaaca tcttgatagc cagttgttcg
    34201 acgctctgtt tgtcccatat catttgtttc acgactattc actcgttctt tacgaaaaac
    34261 aaaataagct tcttcaaatg aaatacgcac ctctttttct ggtaatcgta atcttttatc
    34321 ctcttcagct gtgagataaa caaaaaaagc aggctgttct tctcgcgaac actctgcttg
    34381 taattgaaca tatcgcccag acacaccatt aatttccttt ggtggattag gttcccgtga
    34441 acccaataaa aatacttgac ctaaaaactc tttcttcata cgcaatgctt tttctgccat
    34501 aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa tatttctaca ttacaatcaa
    34561 acaagcggtt aaaaatccta accgcttgtt cctcatgact aatctttggc tttacgtact
    34621 ttataaagtg ttatacctaa agcaatgact aatactacta atccaagaac cataagtata
    34681 ctacttgttt gttcacctgt ttttggcaac attggtacaa cagtagctgg tttaacccgt
    34741 tgttctggtt tcactggttg cggtggtttt ggcgcttcca caatttgaac cgtttgatct
    34801 ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa acgtttcttt ctcatcttcc
    34861 gttttcgctt ggagccattc cgcaaaactc aagccttttc ctgcaagatt gcgaccgtcc
    34921 actaagaatt caaataagaa ttccatcatt tctttatcgg ccgcaaagac tttttccgca
    34981 tgagtgcctt caaatggtgt gccgtctggt gttaagtagc cttgaacaat taagcgatag
    35041 atttgaccaa tcgcaagacc gtctactttg acccattcaa gcacagtttg atttggcaag
    35101 gccaagatct cttttccgcc atttttattc gtcgctttcg tcgtaatggt tggattactg
    35161 attttgatgg tttggtcatt gtcggtaata tctgcgtgag aagccaataa tacgccttct
    35221 ctaaacagat cttcaaagac cacgacttct ttgcctcgca acgcacgtgc gtctagaata
    35281 aagtctagat tgactgtacc atccgttgtt tctgccacaa acgttaaggt acttgtgact
    35341 tcttttccat ctactagcac tggtttttcc gttactttgt ccattagaac accgttgact
    35401 gtgtatgttt ttccaacgac caaatcgtgg tatttcacag cgtctgtaat ggtgactttc
    35461 gttaatggat taacctcttt caagccagtg ggtttatgtg tagccgttgt acgtacactt
    35521 gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt ctttatggtt tgctagttct
    35581 tcatcatttc taaaaagact ttcaaacacg acgatttcac gaccatttat acctgtcaaa
    35641 tcaaaggtaa atggtacgat caccgtttca ttttctgctt tggcaataaa gccaacactc
    35701 gtttctaccg tatttccatg cacaagcaag ggttcaccgg ttgctttatc cattaagatc
    35761 cctttgacca agtatgcttt tcctggaaga atctcggtaa tttcaacggt atcgtttaag
    35821 gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt caaagacagc cgtggtatga
    35881 acggttggat tattcacttt cacactttgg gcttcatcgt tgatatccgc atgtgctgca
    35941 acttcaatgc catcacggaa aagtttttca aaaacaacga tcgtttgacc tttcagaaca
    36001 ctagcattaa ccacaaattc aaggtctttt gtgccattga ctttgtctgc aataaaggcc
    36061 aaggtacttg tgatttcttt gccatcttgt aaaattggcg cattcgttgc cttgttcatc
    36121 aatactcctg aaatggtgta ttcttttccg acaaacaagt tggtataaga aacactgtct
    36181 ttaatgacga tcgtttctaa tggatcaacc actttttcac catcggcttt atttgacgct
    36241 tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag aaaccactaa cgtttcccca
    36301 tcatctggcg cattttgttt gaatgcaacc gtgaatgctt gtttatctgt atttggttgg
    36361 aaactttcta atggggcatt ttccaagaag taatagtcat cggcaactag acctttcact
    36421 aaaatttccc cgttttgatc agtggtatat tcaccaattg cttgattttt accgttatac
    36481 aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt tccattcttc atacttgaat
    36541 aattttacac ttttccgtgc ttgacggttc gcaaaatgga tcactttgat cggttctgta
    36601 tcattggtaa tcgagaacac taatgggttt tgattcaaaa cgtaatttac gggggctttg
    36661 atttcttcca aacgatagcg atcttttcca taagtaagtg taccgttagt taggaaaaag
    36721 cctttttcat tggttttaaa gatatccgtt gtttctgtat catttggaat ctccattgtg
    36781 acataacgat tggctaaagt atcaaaaatc ttgaattctg tattagcaag tggaatcact
    36841 tttcctgttt cttgatccac tttcaccact ttaagacgtt gttcaattac tttatcttct
    36901 aaaacataat ggaaagtttg cccattttca gaaattgtga cttcaagtgg ttcaaatagt
    36961 ttataccctt cttttccttt ggtttcagaa agaatgtacg tatcaaagac aacggtattt
    37021 ttaccaaaag tagcataacc atcattgtct gtcacaacgg ttttcaccac tttgccagtt
    37081 gtttttgacg tcaaagtgat ttcaatgcca gctaattttg gtttgttatc tggattcgtt
    37141 gaatcgacta aatctttatt agcaactttc actaaatcaa tggttccttt gatcacttga
    37201 tcttttgcgg tcgcattgac agtttttcca ggttcaacaa caatttcaat tgcttcatca
    37261 gaaagcacat aaccttttgg cgctttgatt tcttttaaaa catatttgcc aggatcaaga
    37321 tctttaattt ctgcctgtac tttgccgtta atttttttca aggtaatttc tttaatcttt
    37381 ttcccatctg cttgatacaa accatagacc gcattttcta aagaagctgc accttgtgct
    37441 ttcacaccag tttccgcatc ttctttaaac acacgaatgt ttccttgttt tagaatttgt
    37501 aaattaataa cagacgcatt aggttctgtt ggatgtaaca caccaattgt ttgagctcct
    37561 ggacgacgat aggctaatgg cgcaccttga actttaattt tattcaattg aatttttgct
    37621 ttatccggtg cctcttttgt gactgtaatc catacttcat tgccgtgaat agatacattg
    37681 acaccttttg gtttttgaat gtctttcaca tatttcaaaa aatcattttt attctttact
    37741 ttcaacgttt cgccaatttt taccgtatgt gtttgattat taaaagttgc tttttcacca
    37801 aagttattaa tcaaacgttg aatttctgct tttcgacgat cataatcaac acggatattt
    37861 ttgattggga tttttgtgcc caaagcttcc caaatcataa attgtgccgc aatgtattgt
    37921 tcatcggatt tgtctttatt tgtgacatat cctaaataag agatcaagct tgtttttttc
    37981 cgtacatttg aatttttcaa atagtttttc aaagcagtag ctgtataaac ttgaccttct
    38041 gcggctgaga caccaggctc aatacaaaac gccacttgtc cattaatctc aaaacgaacc
    38101 atataattta aagtgaagtt tagattgttc ggactagaag taacaccaat attttcgccc
    38161 caatcaattt tccgaccacc taaaacagct gcattggttg cacggcgaac acgaaccgaa
    38221 cgattggttg gtactgttac taacagtgta ggtgtacctg tttttggaat gttctgctct
    38281 tttttctttt gattttcaat actagccgta gattctttgt ttggtttcgt tggttgattt
    38341 tgactagctg cggttgaaga gattgttttt tcaacgtttt cctcattttt tccttctttg
    38401 tcagcagtag ccgctggttg ttccactgtt tcttctattc gtttcttttc ttccgcttgt
    38461 ttctcagaag actctggctg cacagaaggc gttgcttcgg cagctttttc tccttctaga
    38521 gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt cttcaactgg ttgtgaagta
    38581 ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct gatcgagagt cgttttggaa
    38641 ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag tggcttcacc agctgggacc
    38701 ccggcttctg ctggttggac ttctgtcact tcagaagcga cttgggcaat ggcttccatc
    38761 gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag cagttcccac caagaaacag
    38821 aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat tcattttcgt ttttgcatac
    38881 acaagaattc ctccttttcc tgagaatttt atataataaa acaaaacgcc gttaccaaaa
    38941 gtgataacga cgtgaactgt ctcttctatt tttatctggc gctcttatct gaacacattt
    39001 catcaatgat tttaccctcc cactataatt acagaacaaa aaaagaacct gcttcgtgga
    39061 aatcaactgt ccatttccca attggaatac cagctttttt ggcaacttca ccatcaccaa
    39121 atctcatgcc ataccaatta ctaccttcat ccttaatttc tttccaacca tatgacatag
    39181 cttcatctaa tgaatcaaaa atcatgacac tattgccaat tgttacagga tctggcttaa
    39241 gatcttctgg aagagaaata tcaatcatac cataatgatc cgattcctca gtattattgt
    39301 cattactagc tactggtttg gttgttctac tggcttattt tgatacaaca agtactctta
    39361 ttcttctttt acttctccgt tttcaatttc ccattcaaac cactccgcaa agtcttgctt
    39421 aaaaccatct actcgatacc tattttgtaa ataggcatct tctgacgtcg taggatctaa
    39481 ataggtaggt ttattctctg gacgagcata gacacctacc catgtttcgt aatctccttc
    39541 aaaataacta tctaccaaat agcctttttt tctaagaatc tcccgaactt gatttttaat
    39601 tattgatact tgttcaaaac catcactcat tctatcacct caaataactc tagattctca
    39661 taaatattcc cgacaatttc aatcctatca atgatctcta acaaatcgct acttatgctt
    39721 ccccattcgt ataagaattt cccttccgca aaataaattt tccataaact tcttcatgat
    39781 cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt cttatctttt agctcagtaa
    39841 atgccataag ttcaacatgt tttccaacct cgaaacaatc acaccaccca tcaaccgtta
    39901 cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt attaaacact aaaactttcc
    39961 gcattttttg ctcattaatt aatcacgcgc gaaatctagg aatcacaact tattcgcttc
    40021 ttctttataa ataaaaaaac tccaataatc ggaaattact atcttttcct cattcctttt
    40081 ttaaatcttc atacttcttc ttatctttgt cctaacctaa taacacaaat tttgaagaaa
    40141 aactcagtaa aacctgattc tttttttatc ttcacaacaa ccaacttttt cttcagatcc
    40201 ttttactaga acttgtatac aaaaattgtt gaatgctgat ataatttgcg gctacttgaa
    40261 aaaacgatag atgtaaaaag ataaatagaa tcatttttta ttctatatca gtctatgatt
    40321 cattgcttat caatcaccca acccattcga tgccgttttt tttagagaaa tttaaaaaag
    40381 accgtacgaa tctcgcatta tccgattact tttccagaaa agatccccaa ttctgtataa
    40441 ataaccctat cttcttgttg gtttcatttt ctcatataag aattcagtca atctacccac
    40501 tatgaccatt actacgtcca acacaactga catattaacc acgtaagtat tccctcacag
    40561 atctccgaat ctccatggta tggattccca tcctgacgct gcagaggaca acatatttca
    40621 tgtggctctg tacccttttg atttggaaac actacaaatc aatactcatc tataacttat
    40681 caagcgaact ttcgctaggc ttctagtgat tactaaattg ttggataaca tacatagccc
    40741 attttatcta gctgttattc ataacagtta gttcttgtat tacttcttcc gcttgtgttg
    40801 ccattgagct tgtcaaaaag ttacttagaa aaaagctgcc agtaaagcaa ctttcaacaa
    40861 accctttttg tttcttatca tatcaagaaa cctcctttta taaatttttt ctataataaa
    40921 aaacgtcgat aacttaataa gtatcgacgt aaactatttt tatatcccgt aaatcaaaaa
    40981 ttctaaagaa agatttccta tataagcata ttggataatt cgattagtat tgggtatatc
    41041 tctaccattc tcatacctat ctatggacca ttttgaaatt gaaggagaaa aaagctttcc
    41101 aaactcctcc ctttctagat aataatcttt cctaattttc ctcatcctta atcccatact
    41161 aaaagaattt atctttttaa aatcgctaac tctttcatct tttggaagta tattcttata
    41221 atcttctccg tacataaggt aaggaacagt gaccttacca agataggcaa tattcattag
    41281 tttttcaata tctggaatat taataccttt ttcccatcta cttataacat ttttgcctgt
    41341 atagatggta aagtttttac caaatttttc aagcgtctct tcccggttct ttcggacttc
    41401 tcgtattctt tttcccataa caattttatc gtcttttgat aaactaaata tctcactatc
    41461 acgattaatc tccttcaaaa tttctttttc attacttaat agaaaaatat cgattttttt
    41521 ttgcatttct tttgacggat ttctctttct atttaacaca taactgagta atacttcact
    41581 aattcctata ttatcagcta actctttgtt aagtaactta ttcatatctc tgtatgcaat
    41641 tagtttaccg atgagaatct caatcttatc ttcctcatct tttattagag tttctatatt
    41701 cagaatcttg cttagagtat tatctacgtc tttagggatt tttcttgacc ctttttcata
    41761 tttaactaaa gtactttgtg aaatacctgc taaatcagct aacttttttt gagatagttt
    41821 tttctctttt cttagagatt taagaaccct tccacttgaa ttatcaacca tgaaaaaagc
    41881 acctcctatg agatgctttc atcatatact gacagctaaa aatcttcaag aaaaatattc
    41941 caattttgtt aattttagta tcgccgtgat aaccaaatga cagtttaaaa tcttttatac
    42001 agataatcct cagtgttgca tctactcaaa aaggtttatt taataggttt ttcgattgat
    42061 tataagttat ttcacactat cttgctcatc cattttcaga acggccatga aggcttcttg
    42121 cggaacttcg acggagccga tttgtttcat ccgcttcttc ccttcttttt gtttctctaa
    42181 gagtttacgt ttacgagaaa catctccacc ataacattta gccaagacgt ttttacgcaa
    42241 ggctttgatg tctgaacggg caacgatttt ttgtccaata gctgcttgga ttggcacctc
    42301 aaattgttgg cgtgggatta gttttttcag tttttcaacg attgcttttc cacgttcata
    42361 ggcaaagtct cggtgaacga taaagcttaa tgcatcgact ttttctccat tcagcaagat
    42421 gtccatcttc actaacttgc ttttttgata accagacatt tcataatcca atgaggcata
    42481 gcctttcgtg cttgatttca atttgtcgaa aaagtcaaag acaatttcag aaagcggaat
    42541 attatagacg acatttactc ggtaatcatc taaataatcc atcgtaatga attctccccg
    42601 ttttcgttga gaaagttcca ttactgcacc gacaaaatca tttgggacca tgatctgtgc
    42661 tttgacgaat ggttcttcta catcctgaat cgttacaggt tcaggaaaat ctgccgggtt
    42721 atctactgta gcagtcgtac catccgtttt attaacatgg taaataacgg atggtgcagt
    42781 cgtgatcaat tctagattga attcccgttc caagcgttct tggacgacat ccatatgaag
    42841 aagtccgagg aatccacagc ggaacccaaa acctaatgct tgagaggttt ctggttcgaa
    42901 ttgtaaagca gcatcattca attgcagttt ttctaaagct tcccgcaaat cgttgtaacg
    42961 agaagtatca attggataaa gaccacaata aaccatcgga ttcatttttc ggtaacctgg
    43021 taaagcttca gcggcaggat tgtctgctaa cgttactgta tcccctactc gtgtatcttg
    43081 gacagttttg atacttgctg tgatataacc gacatcaccg accatcaaaa agtctctagc
    43141 aactgctttt ggcgagaaga ccccgacctc tgtaacatca aatgttttgc cattactcat
    43201 cagtttgatt ttgtctccgg gtttgaccat accgtccgtg atccggacat ttagaacaac
    43261 accgcggtaa ctatcataaa tagagtcaaa gatcaaagct tttagcggtg catctaagtc
    43321 tccacttggt gcaggaacat attccacgat ctgctcgaga atatcttcaa tcccaatccc
    43381 tgatttggca ctggctaata cagcttcact agcatcgatt ccgattacgt cttcgatttc
    43441 ttgacggaca cgttcgggat ctgctgcggg taggtcgatt ttattgatga ctggcaggat
    43501 ctccaaatca ttgtctaacg ctaaataaac attggccaat gtttgtgctt cgattccttg
    43561 tgctgcatcg accactaaaa cggcaccctc acaggcagca aggcttcgag agacttcata
    43621 ggtgaagtcg acgtgccctg gggtgtcaat caagtgaaag atatatgttt ctccattttt
    43681 cgctgtgtaa ttgagttcta cagcatttaa tttaatcgtg attccacgtt cacgttccaa
    43741 atccatcgag tctaacagct gatcttgcat ttcgcgagac gtcacagtgt gcgtcatttc
    43801 taaaatacga tcggccaatg tagatttccc atggtcgata tgagcgatga tcgaaaaatt
    43861 acgaatctgc tcttggcgtt ctttcatttt ctttatatcc atagcgtctc ctcttttcat
    43921 ataatcagca ctttccatta taccaacgaa aagcagtaat ttaaagagtt tttaattagt
    43981 cagctataga agtttttttt gtagaatttt gctatactgt tatcaagcta ataaatggag
    44041 atgaaaaaat ggatcaaaaa gattttagag aaaatcttga attaaaaccg gtcactcaag
    44101 aacatatcga tcaattcaat gaattgcttt cctatgtgtt ccaagtgact gaagcagaca
    44161 tcgaggaaag cgggtttgag aataaacgag cttttatccg ttccaaaaaa ccgattttgg
    44221 aagtatcgaa agtgttcgga tggttccacg aaaataaact gatctctcaa atcgctatct
    44281 atccttgtga agtcaatatt cacggtgcac gttataaaat gggtggtgtg actggagttg
    44341 gaacttatcc agaatatgct aaccatggat tgatgcaaga tctaattatc gttgcattgg
    44401 ataatatgcg caagaataag caatggatct cttatttgta tccttacagt atcccctact
    44461 atcggcgtaa aggatgggaa atcatgtctg ataaactttc ctttaaaatc agagatacac
    44521 agctacctaa acaaatccca gtaagtggta tggttgagcg attaccagtt gatcatccag
    44581 acgtattttc cgtttatgac aaatttgccc aacaaaacca tggtgcttta ttccgcagtg
    44641 attttcattg ggaagagtat tggcgtttcg aaaatgaaga tgagcgcaca gccgctgtct
    44701 actacgatag caaccatgaa ccaagaggtg ttctgttcta ctgggtagct gaagaaatat
    44761 ttcacgtaaa agaaatgttc tatcttgatc aagaagctcg aaatggctta tggaacttta
    44821 tttccgcaca tttttccatg atctactggg tacatggaga tatttataaa aatgagcctt
    44881 tagcattttt gattgaagat agtcagatca aagagcagat cgaaccatat tttatggctc
    44941 ggatcgttga tgtaaaagaa ttccttcaac ggtttccgtt tgttggaaca gctgatgctt
    45001 ttcatttcat cattgaagat cctgtcgctc cttggaataa tggtgtcttc gctttaacat
    45061 gggatgaaca gggacaagtc cgtgtcttaa atgaacctat aggaaaatcc gttcgactgg
    45121 atatccaaac attgacttgc ttgatgatga attatcgccg tgcttcttat ttggcaagaa
    45181 tcgaacggtt agaaacagat gaagaaacac tgaagtcact agagcgtatc attccaaaca
    45241 tggaagctta ctttagcgat tatttttgat aaaaaggatg atgtttagat gaaaatttat
    45301 tttgccgcac caatgtttgc taaaagtgat ttgttatata accaacagtt agttaaagaa
    45361 atcagagagc tctcttctga gctttcgatc tatcttcctc aagaaaatga agcaatcaat
    45421 gacaaaactg cttatgcaga cagtcgaatg attgctcttg ctgacacaga aaaagtgttg
    45481 gctagtgatt taatgattgc tttactagat gggctgacga tcgatgccgg ggtagcatcg
    45541 gaaatcggtg tggcttatgc gaaaggcatc ccagtgatcg gcttatatac tgatactcga
    45601 cagcaaggcg gaacccaccc gaaaaaaatc gctgcactac aagaaacagc tgaaaatcaa
    45661 tttcactacc tgaacttata tacaataggt ttgatcaaat tgaacggaaa agtggtttct
    45721 tcagaaattg agttgctttc agaagtaaaa agatttttag atggagggac tttcagtgat
    45781 taaagaaatc aaaaaggtgc aaatagcttt acttgctttt ggtgtattcg ttgtctgcta
    45841 taatttttat gaatttatca cacaaaaata ttcgacttct caaggaatca catttatcgt
    45901 ggaatcttta ttagggattg cattgatttt catgccacaa gtcattttga aagttttcaa
    45961 acttaaaata ccagcagcaa tcgttttgtt ttactggttt ttcttgttca tctctgtctt
    46021 tttaggcaca gggatgcatt tgatcagtat catctctttt tgggacaaaa tcttacatgc
    46081 ggtgagtcca atggtcctga cagctcttgg ctatggattg attggttact tgatgaaaga
    46141 tgctgagata tcaaaaacca gtccttggtt gttcctctta ttcggctttg cgtttgctgg
    46201 actttgcggc gtgttttggg agttctggga attcttatgc gaccagttct taggcatgaa
    46261 tcttcaacgt tttgctgctt ctgatggcac actgtttgtc ggacgagcag cattgatgga
    46321 tacgatgggc gacttgctga caaatacgat aggtgctgcc ttaatgggat tgtttgcatg
    46381 gtctcaaagt aaaaaagatg agcgttactt tgaaagttat aaattagaaa aagtgaaaaa
    46441 tacctaataa aagaggttgt gacagaagcg ggcagcttca agaaatttcg gcttattttt
    46501 cgaagaaact acttctggaa cactgtttat ttgaaaaaag gaggtctgac actagttgtg
    46561 tcagacccct tttttctttt atagtctcaa tggaagatta gacgatttct tctagctctt
    46621 ctgttttgaa atgaagttgt ccatcaagta aagtaatcgt tacttttgat ttaggcataa
    46681 catgtcctgc aacgatttct ttagctagtg gtgtttctac ttccttagtg ataaatcgtt
    46741 tcaatggtct tgcaccatat gctggttcat aagcattttc agcaatccac gttttggctt
    46801 catcactgat tgtcagcagg atttcttgat gttccagacg ttgtgccaat tgcgccacca
    46861 ttttgtcaac gattcctttc acgttgtcta ggctcaatgg tgtaaataaa atcgtgtcgt
    46921 cgattcggtt caagaattct ggtttgaagt ttccacgtaa tagcgtgttg acttgttcag
    46981 ccactgcttc tggaatcgtt ccatcagcag tcacgccttc cagcagtaac tgtgaaccga
    47041 tattgctggt catgatcaga accgtatttt tgaaatctac tactcgccct tttgaatcag
    47101 tcaagcgtcc gtcatctagg acttgtaaca agatattgaa gacatcggga tgcgcttttt
    47161 cgatttcatc caacaagacg attgtataag gatttcgtct gacagcttcc gttaattgac
    47221 caccttcttc gtaacctaca tagcctggag gagctccgac tagacgagac acagcatgtt
    47281 tttccatata ttcactcata tcgatccgta ccatatgatc ttctgagtca aacaagttct
    47341 cagctaacgc tttcgctaat tcggtttttc cgacaccagt tggtcctagg aaaaggaaag
    47401 aacctagagg acgatttggg tcttgcaatc cagcccgtga acgaattacc gcgtcactga
    47461 ccgcatcaac tgcttcatct tgaccaatca ctcgtttatg aagtgtttca ttcaatttaa
    47521 ttagtttttc tcgttcacct tctacaagtt tcgttacggg gatacccgtt aatctgccaa
    47581 cgacttgagc aatctcattt tcggtaacgg actcttgaac catcttaatc tcgctgtctt
    47641 ttgctttagc ttctaattcc ttcaattctt tttctaattg aggtatcgta ccatgacgca
    47701 atacagcagc gcgttctaaa tcatagttat tttctgcatc ttctaattcg tgttttgctt
    47761 tatcgatttc agcacgtttt gctgaaacag aattgacttc ttctttttct gtttcccact
    47821 gcatcttcat cgcatttgct tcttcacgaa gttcagctaa ttcttcttgc aaatttttca
    47881 agcgtttttt acttgcatca tctgattctt ttttcagtgc agcttcttcg atttccaact
    47941 gcatcaaacg tctagtaact tgatctaatt ccgttggcat agagttcatt tctacccgaa
    48001 tcgttgcact ggcttcatcg atcaaatcga ttgctttgtc cggtaagaac cgatcagtaa
    48061 tataacggtc agataaagta gctgctgcta ctaatgcgtt gtcatggatg ttaacaccat
    48121 ggtggatctc aaaacgttct tttaatccac gcagaatact gatcgtatct tccactgttg
    48181 gttctttgac taataccttt tgaaagcgac gttccaacgc tttgtctttt tccatatatt
    48241 gacggtactc atctaaagtt gtggcaccga tcaaatgtaa ttcaccgcga gcaagcattg
    48301 gtttcaacag atttccggca tccatacttc cttctgtttt tcctgcccct acgatattgt
    48361 ggatttcatc gatgaacaag aggatacgtc catcactttt tttgacttct ttcaagactg
    48421 cttttagtct ttcttcaaat tcaccacgga attttgctcc agcgatcaat gcacccatat
    48481 ctaatgaaaa aatcgtttta tcttttaaat tttcaggcac atctttgcgt acgatacgtt
    48541 gagcaagccc ttcaacgatt gccgttttcc ctacaccggg ttctcctatc agaacaggat
    48601 tattttttgt ttttcgtgaa agaatacgga tgacatcacg gatttcttca tcacgaccga
    48661 tgattggatc catttttccg cttttgactt gctgtactaa atcaacgccg tatttttcta
    48721 atgctttgta ttgttcttct tggttttgtg aggtcactct ttctcctcct ctcatctctt
    48781 cgatgttttt acgcaattct ttttcagata atccgttttt attcaaataa acagttaacg
    48841 gataattttt taatttcatc aacgctaaaa taacgacttc cgttgaaaga aactcgtcgc
    48901 cgaagctttc acgtatttgg tctgcttcat tcaataatcg aaagaaattc tggctgaggt
    48961 tttgtccata ttggacgttt ccaccagaaa caactggata ctcatccagt agacgatcaa
    49021 tttcatgttc aaaagattcg acatccaatc cggcatccgt atagaaattt cgaccaaaat
    49081 gattagattg taaaaagatt ttccatacat gggcaatgtc gatatcttga tgttttcgag
    49141 tgaccgcaat ctgttgagct tcagcaatcg cttcttgaag cgtcgtagtc attttctcaa
    49201 tattcataca ggtataccct cctaattaaa ggtaagatca cttatttctt tgatctttac
    49261 aaaaactatt atatcgcttt ggtcaatttt ggtcaaaata tacacatagt tttttgactt
    49321 tttttctcga cttatataaa tcttaaaaaa acaaaattga ttgtatgtat ataaaaaaag
    49381 agccaaacaa acgtaaaatt atcttatcca taacgttttt ctttgatttg ataaaaaggg
    49441 tagatcttat aaaagaccta cccttgattt tctttattct ctttttttac ttctattatt
    49501 tcttctttga tcactcttcg ccgttcagca atcacataca caaatccata gagaaatggc
    49561 aagataaagg aactgaaacg atacagtaaa agcgaagaaa ctgcgtcaac tgttccaaca
    49621 actggtctga ataataacaa atagacaaat tcaaaggaac caataccagc aggcgtagga
    49681 ataacccctg aaagcactac tgcaaagctg ataaacgaaa tggtcagtag aaaatctatt
    49741 ttaggatggt tttcaatcag aatcaaataa ggaagtacat accaaaatac aagttttaac
    49801 atattccata gataaatccg caatagcgca gtcctgtctt ggataatggt ttggaccgtt
    49861 tcccttaaag agtagatctg ctgattgcat gtatccaccc atttccgcaa acgctcactt
    49921 ttaaataact tattcgataa gctgacaaaa aatatttgaa cattcaagct gacggacatc
    49981 gccaataaaa aggcaacaat cacgatcgtc aaaaggatac cagcgatgat aaaccagatc
    50041 attgatggac cattagaata aaaaagagag aattggatga ccaagccagc taatgcataa
    50101 gtgatcactg ccagtttgta cataatcata tggagtgctg tcacccctgc tccctgggat
    50161 agcttcattc cttttttgtt ataaaaataa atctccgaga tcagcgttcc tgtaccaaac
    50221 gaaacgattc ggtaaaacgc aatatagcta gaggtcaaga acccatcttt cgttgtaaaa
    50281 ttcggcgcga aaggttcagc aatttctttg attgagcgcc cttcagcgat ttggtaaact
    50341 gtccctaata taacagccgc tgccaaaaca acaacactgg tagacagcaa ttcggcaaag
    50401 atatctgcaa atgattgatt gattaaataa taaataatgg caaaaatcac aatcagcaaa
    50461 agcagattca gaatgatttt agttttcgtt cctttagacg acatctcctg ctccttaatg
    50521 gaaaaaataa atgagaatca aatcaacaag tacacctgca gcgaatccta tagtaaactt
    50581 ctttttccga gttttatgat ggaaaacttt tcgagagatc aatcctccca aaccaccgcc
    50641 taacaggctg acaaatatca aattattctc aggaacacgc cagccgctag ttctagcctg
    50701 atgtttatca taccccatca gacaaaataa gtaaaaattc acaatgaaaa ggtaaaacca
    50761 caaaggattg tgtaacaata catccattac tcttcttcca actttctgtt aactactatt
    50821 tctaagcttt ttcttctttc tttggtgaat aaactgttaa tccttttgat acaacagata
    50881 attcaactgg aagatcatct gtcgtgtctc catccgttcg agtgcccaca gatttttcct
    50941 gcgcttggat tttcatgcgt ttggctgtga aataagtaat gccggggatc gtatagaatt
    51001 tgcctctcat gattctaggc aaataaaaaa taaatcggaa aatatctaag gaaggtacga
    51061 tcgttacatg aaattttcca tcatctaggg tcgctgagtc gtcaaaattt gtaaaaccgc
    51121 ccacggagtt agacatcgtg actgtcaaca gttgggattt tcctttccaa tgctcctcgt
    51181 ctgtttctaa atccaaatga tattttttct tcttcagcaa taacttaaaa aacttcttga
    51241 taaagatcca cgagccgtaa cgctgttttt cttcttgact gatccaaact gctgtatctg
    51301 ctaagattcc gattgtcagt gtagagataa tgacttcatc attgacttga ccatagtcta
    51361 ttttccgtac attgccttcc aggatcacgt cagcagcttc ttcttttttg aggggaatct
    51421 caagaacacg tgcaaggttg ttgacagttc cacctggaag taatccgaca tggtattgag
    51481 ataatgtttt ttggaacgct tggacgatat gatgaatcgt gccgtctcca cctataatta
    51541 ctaaagtatc agcttctatg gcttctgctt tcttgactaa ttccgcatca tcaatcgatg
    51601 ggccagtttc agctagttct acatataatt ctggctgctt ttcttttaca taatcttgaa
    51661 accagcgtgc taatttctca ccctcatctt tccctgaact tttgttataa aaaactagca
    51721 gcttttccat tctttacctc ctatttactc ttcaaaaaag agggcgggac aaccaattcc
    51781 attgtcctgc cctttagcct tagacagatg acattccatc attgggacga acgccttgtc
    51841 ataagctgtt ctttttcgat tttaactctt tgtgttcaat tctgcaattt ttacaatcac
    51901 gtctgttgct ttttccatta cttgtaaaga aacaaattcg aatcgtccgt gcatattttc
    51961 tcctccggca aatagatttg gcgtcggaat acccatgtaa gagatttttg aaccatctgt
    52021 tcctccacga actggttcga tcactggaga gataccaagc tcgatcattg cttgtttcgc
    52081 taattccacg atgctcatat ctttttcaat gatttctttc atgttgtaat attgatcata
    52141 catatctact ttgattcgtt cttgatcaaa tcgctgattc aactcttctt ggatatgtgt
    52201 gatttgtgct ttgcgtgctt cgaatttttc acgatcatga tcgcgaatga tataactcat
    52261 agaagcttct tcaggattcc catccaatgc catcaaatgg aagaaacctt cgtatccttc
    52321 cgttttttct ggaacttcat cggctggaag ctgattatga aaatcgattg ctaactgtag
    52381 agcattgatc attgtatctt ttgccgtccc tggatgtaca tttttccctt ggatcgtaat
    52441 atttgcttga gcagcactaa acgtttcata ctgcaactca cctaaaggac caccatcgac
    52501 tgtataagca aaatctacat cgaattgctc aacatcgaat ttatccgctc cgacaccgat
    52561 ttcttcatca ggaccaaaag ccactttgat ggtaccgtga gggatagaag gattattgat
    52621 caagatctcc attgcagtca tgatttccgc aatccctgat ttgtcatctg ctcccagtaa
    52681 tgtactgcca tctgttgtga tcaatgtgtg gccttcataa tttttcaaat ttggaaaatc
    52741 ttttgtattc aatgtatagc gttcttccgc atctagtacg atcgtagact cgccatcata
    52801 gttttcaatg atttgtggat tgactcctac tgcattaaaa tctgctgtat ccatgtggga
    52861 aataaatcca atggaacgca cttcatgatc aacattgctt ggtaatgttc ccataacgaa
    52921 tccgttatct tcattataga aaacatcagc taagcctaac tcttctaatt cttttttcaa
    52981 ttcttgcgca aaagcaactt gtgtctgagt cgatggagtc gttttgcttg ttgcatcgga
    53041 gcgtgtttct gttttgacat atcgtaaaaa acgtggcaaa agattttcgt acatagtatt
    53101 ctctcctttt aattaaattg aaatgggttt gtatttgttt cagaaacgaa aaagttaata
    53161 tcccaatttt cttcttgttt ccaagattca aatttctcga taaatcgttg cttgcataac
    53221 gattcaatat aatgaccggg atcaattgca atcaacccag cgctttgcat atcatgagcc
    53281 gtatgataat agatatctcc cgtgatatag acatctgctc tttgggcgat tgcttgcgga
    53341 taaaattttt caccgcttcc accgcaaatc gcaatgcgct tcacagagga ttttgcattt
    53401 tttggctgga ctattctcag tccatctagt tggaacgctt cttttacttg ttctacaaat
    53461 gcctctatag aaatttcttg cggcaattca cctactcgtc ctaacccaaa catttcgact
    53521 ggttcatcta tagcgaaaag atcataagca ggttcctcat aaggatgagc tgaccgcatg
    53581 gcttgaatca cttgtttttc gatagtttcc ggcaaaataa cctcaacttt tgcctcttga
    53641 acttgttctg tcttccctac tttcccaatc gctggttgtg caccagcttc cggagtaaat
    53701 cgaccgtgtc caatcgaggt aaaacttgta ccggtataat caccttgtgt accagcacca
    53761 gctgcagcta atacttcacg cattttctgt gcatgatcca caggtacata aacggccagt
    53821 tttttataat gaatctcatg agtctttact aaataactct ccacttcaat accgagaagt
    53881 tcacaaaacc agtcattcag tccatcccaa ataatatcca tgttcgtgtg tgctgcatag
    53941 acggcaatat catgtttcaa caaatctgca tacatttttt cttgtggctg atcagtaacc
    54001 agccgtttta caggacgaaa gattggagga tgtttagcaa tcagcaaatc gatttttttc
    54061 ttgattgctt cttcgaccac ttctggacga acgtctaatg tcatcattac tcgttggatt
    54121 ggcttatcca atgttccgat atgcagtccg actgggtctc cttcttcagc tagccactga
    54181 ggacaatagc tttcaaactt ttggatgaaa gtgcgtccgc tgattgtcat ggctttaaaa
    54241 cctcctcaat catttggatt tcctgctcga tttgtttttg acgtgcctct tgattagctg
    54301 cttgtttgag ctgttcacgg atagcttttc tttgttgaag ctctcttgtc cattttgatt
    54361 tgaagatctc acttttctct tcaagcagaa atggcccgaa catcagctcc atttcattgt
    54421 aagtaactgg tttttgttct ttttcggcta caatgatttc atatattttt ttattttctt
    54481 caagtatttt ttctgattgg attttatact gattttcttg cagccaagat cgtaatgtac
    54541 gctctccgat gtttggctga aggatcaaac gttcttgtcc gcttaagcgt tgattcaact
    54601 tgccagcttc taggatatct cggatcagtg aacctcccat acccgcaatc gtgacagctg
    54661 taataccatc ttgtggctgg atagcatcta gaccatttgc aaggcgtacg gtgatacggt
    54721 cttctaatcc atcttttttg acttgcttac gtgctgattc ataaggacct tgaacgactt
    54781 caccagcaac tgcatattcg atttttcctc ttaatatcaa tgcaacgggt aagtaagcgt
    54841 gatctgatcc aatgtctgct aaacgagcgg attcagggac aaagtcaccc actgcggcta
    54901 agcgctgaga taaatctaaa tggttcatag cttcactcct tctagataat tatagcattt
    54961 tccaaataaa ataaggagac taggaataaa aaaattccca tatctcctta aaaaatatac
    55021 cttttcgttc tataaaacaa cattttctta tttttgaaaa aatgttattg gttatttgtt
    55081 c
//
LOCUS       DACEDD010000013        54793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEDD010000013 DACEDD010000000
VERSION     DACEDD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.013"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            8..586
                     /locus_tag="IWO45_000908"
     CDS             8..586
                     /locus_tag="IWO45_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332398.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(826..909)
                     /locus_tag="IWO45_000909"
     tRNA            complement(826..909)
                     /locus_tag="IWO45_000909"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(873..875),aa:Leu,seq:gag)
     gene            1292..2509
                     /locus_tag="IWO45_000910"
     CDS             1292..2509
                     /locus_tag="IWO45_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0332399.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKIREFLATYRQK"
     gene            2693..3529
                     /locus_tag="IWO45_000911"
     CDS             2693..3529
                     /locus_tag="IWO45_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0332400.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3605..4753
                     /gene="nagA"
                     /locus_tag="IWO45_000912"
     CDS             3605..4753
                     /gene="nagA"
                     /locus_tag="IWO45_000912"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0332401.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4974..6032
                     /locus_tag="IWO45_000913"
     CDS             4974..6032
                     /locus_tag="IWO45_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0332402.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6051..7070
                     /locus_tag="IWO45_000914"
     CDS             6051..7070
                     /locus_tag="IWO45_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0332403.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7172..7888)
                     /locus_tag="IWO45_000915"
     CDS             complement(7172..7888)
                     /locus_tag="IWO45_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0332404.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7857..8642)
                     /locus_tag="IWO45_000916"
     CDS             complement(7857..8642)
                     /locus_tag="IWO45_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0332405.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8633..9382)
                     /locus_tag="IWO45_000917"
     CDS             complement(8633..9382)
                     /locus_tag="IWO45_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0332406.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9680..10204
                     /locus_tag="IWO45_000918"
     CDS             9680..10204
                     /locus_tag="IWO45_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332407.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10220..11719
                     /locus_tag="IWO45_000919"
     CDS             10220..11719
                     /locus_tag="IWO45_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0332408.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11807..12409)
                     /locus_tag="IWO45_000920"
     CDS             complement(11807..12409)
                     /locus_tag="IWO45_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0332409.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12513..13469
                     /locus_tag="IWO45_000921"
     CDS             12513..13469
                     /locus_tag="IWO45_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0332410.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13774..14730)
                     /locus_tag="IWO45_000922"
     CDS             complement(13774..14730)
                     /locus_tag="IWO45_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0332411.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14904..15464
                     /locus_tag="IWO45_000923"
     CDS             14904..15464
                     /locus_tag="IWO45_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332412.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15457..16282
                     /locus_tag="IWO45_000924"
                     /pseudo
     CDS             15457..16282
                     /locus_tag="IWO45_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            16292..16909
                     /locus_tag="IWO45_000925"
     CDS             16292..16909
                     /locus_tag="IWO45_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0332413.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16927..17610
                     /locus_tag="IWO45_000926"
     CDS             16927..17610
                     /locus_tag="IWO45_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0332414.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18293..19576
                     /locus_tag="IWO45_000927"
     CDS             18293..19576
                     /locus_tag="IWO45_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0332415.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19592..20479
                     /locus_tag="IWO45_000928"
     CDS             19592..20479
                     /locus_tag="IWO45_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0332416.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20496..21332
                     /locus_tag="IWO45_000929"
     CDS             20496..21332
                     /locus_tag="IWO45_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0332417.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21335..21958
                     /locus_tag="IWO45_000930"
     CDS             21335..21958
                     /locus_tag="IWO45_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332418.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21955..23247
                     /locus_tag="IWO45_000931"
     CDS             21955..23247
                     /locus_tag="IWO45_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0332419.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23606..23703
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23819..24400
                     /locus_tag="IWO45_000932"
     CDS             23819..24400
                     /locus_tag="IWO45_000932"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0332420.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24403..25710
                     /locus_tag="IWO45_000933"
     CDS             24403..25710
                     /locus_tag="IWO45_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0332421.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26126..26614
                     /gene="purE"
                     /locus_tag="IWO45_000934"
     CDS             26126..26614
                     /gene="purE"
                     /locus_tag="IWO45_000934"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0332422.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26598..27731
                     /gene="purK"
                     /locus_tag="IWO45_000935"
     CDS             26598..27731
                     /gene="purK"
                     /locus_tag="IWO45_000935"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0332423.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27744..29039
                     /gene="purB"
                     /locus_tag="IWO45_000936"
     CDS             27744..29039
                     /gene="purB"
                     /locus_tag="IWO45_000936"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0332424.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29219..29596)
                     /locus_tag="IWO45_000937"
     CDS             complement(29219..29596)
                     /locus_tag="IWO45_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332425.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29852..30580
                     /locus_tag="IWO45_000938"
     CDS             29852..30580
                     /locus_tag="IWO45_000938"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0332426.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30580..30834
                     /gene="purS"
                     /locus_tag="IWO45_000939"
     CDS             30580..30834
                     /gene="purS"
                     /locus_tag="IWO45_000939"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0332427.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30836..31507
                     /gene="purQ"
                     /locus_tag="IWO45_000940"
     CDS             30836..31507
                     /gene="purQ"
                     /locus_tag="IWO45_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0332428.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31508..33730
                     /gene="purL"
                     /locus_tag="IWO45_000941"
     CDS             31508..33730
                     /gene="purL"
                     /locus_tag="IWO45_000941"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0332429.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33715..35154
                     /locus_tag="IWO45_000942"
     CDS             33715..35154
                     /locus_tag="IWO45_000942"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0332430.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35186..36229
                     /locus_tag="IWO45_000943"
     CDS             35186..36229
                     /locus_tag="IWO45_000943"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0332431.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36226..36804
                     /gene="purN"
                     /locus_tag="IWO45_000944"
     CDS             36226..36804
                     /gene="purN"
                     /locus_tag="IWO45_000944"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0332432.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36801..38336
                     /gene="purH"
                     /locus_tag="IWO45_000945"
     CDS             36801..38336
                     /gene="purH"
                     /locus_tag="IWO45_000945"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0332433.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38352..39626
                     /gene="purD"
                     /locus_tag="IWO45_000946"
     CDS             38352..39626
                     /gene="purD"
                     /locus_tag="IWO45_000946"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0332434.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39748..40662
                     /locus_tag="IWO45_000947"
     CDS             39748..40662
                     /locus_tag="IWO45_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0332435.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40795..42360)
                     /locus_tag="IWO45_000948"
     CDS             complement(40795..42360)
                     /locus_tag="IWO45_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0332436.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42353..43804)
                     /locus_tag="IWO45_000949"
     CDS             complement(42353..43804)
                     /locus_tag="IWO45_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0332437.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43818..44834)
                     /locus_tag="IWO45_000950"
     CDS             complement(43818..44834)
                     /locus_tag="IWO45_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0332438.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45054..46142
                     /locus_tag="IWO45_000951"
     CDS             45054..46142
                     /locus_tag="IWO45_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0332439.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46196..47722
                     /locus_tag="IWO45_000952"
     CDS             46196..47722
                     /locus_tag="IWO45_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332440.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47724..48929
                     /locus_tag="IWO45_000953"
     CDS             47724..48929
                     /locus_tag="IWO45_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0332441.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49186..50163
                     /gene="guaC"
                     /locus_tag="IWO45_000954"
     CDS             49186..50163
                     /gene="guaC"
                     /locus_tag="IWO45_000954"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0332442.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50427..51851
                     /locus_tag="IWO45_000955"
     CDS             50427..51851
                     /locus_tag="IWO45_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0332443.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52312..53421
                     /gene="pdhA"
                     /locus_tag="IWO45_000956"
     CDS             52312..53421
                     /gene="pdhA"
                     /locus_tag="IWO45_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0332444.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53424..54401
                     /locus_tag="IWO45_000957"
     CDS             53424..54401
                     /locus_tag="IWO45_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0332445.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54426..>54793
                     /locus_tag="IWO45_000958"
     CDS             54426..>54793
                     /locus_tag="IWO45_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ0332446.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFK"
BASE COUNT        17521 a         9382 c        12009 g        15881 t
ORIGIN      
        1 tttagacatg aatatcttat ttttattgct atttttctta attttccttc taacatatag
       61 gaaatgtcaa aactattatg tgcgtatatt gactgcatta ccgtttttct taaacctgat
      121 tatttatttt ggtaatacaa tgggtcaaag ctttacttac gtcaatggaa ataaaagggc
      181 aatgatttgg agtagtagta acctgaataa tctctttaca gagttaggaa cgaaactgtc
      241 acttttttat ccaggtactt ggatagctac tttagtagtc ttggcattat tactatgttt
      301 aattgttgga atttatttaa gttttgacaa taagaaaact tctatttttt tagttatttt
      361 gatgataatg ggattttgtt caaggttaat tatgggattt tctcccacag tatgggcttc
      421 cggaatgcgg acgtactata tactttatgt tgttatcgcc atcttagtac taatggcagt
      481 taaagagcta atgaaaagca tgagtgtaca aaaaaatgaa tttatgcaat ttggattaac
      541 cgtgcttggt atttgtacct ttataataac ggtgataaac agataatata caaaaaatcc
      601 ctcgctttag tattaggtga gggattttac tcgtttttcg attaattgtt ttaacacttg
      661 caagtttcta cgagtagctt gtttccgacc ttcatatact cacctcaggc tgtttacatg
      721 attgtacgtt ctttttgtaa aggatacagt gatatgtata taatggcata aagaaacgtt
      781 caaacaaaaa aactcgtgaa gcaaaattaa cttcacgagt tgtagtgcag atgatgggac
      841 tcgaacccac acgagctaac gctcacatga ccctcaatca tgcttgtctg ccaattccaa
      901 cacatctgcg taataataac acactaatga tagcgaggtt ttacatagtt gtcaagctca
      961 aaaatgggag gacttattaa gatggagaaa tatgacggag aattttcagg actgggaatg
     1021 atcttaggtg tattgattgg acttgcattc ggacgttttt tgcttggttt tatgctagga
     1081 atcatttgtg gaattgcaat ggattgggca gcgaatctat ggaatgacta tcatgacaat
     1141 taagttctca acgcttgttt gtgttttttt aatacttacg tttttcctaa ataagtggca
     1201 aaattttcta ggatggagta tgatattgaa atagtaaata acaaaaaatt tggaaagttc
     1261 taactttttg aaatgaggga ttggctaagt gatgagagaa tttgaaaaat ccaacaaatt
     1321 agaaggtgtc agttacgatg ttcgcggtcc cgttctagaa gaagcggagc ggatgcaaga
     1381 agagggcatt agtattttaa aattgaacac gggaaatccc gcaccttttg gatttgaagc
     1441 accaaacgaa gtcattcgtg atttgatcat gaatgcccgt gactcagaag gctactcaga
     1501 ttccaagggg atcttttctg ctagaaaagc aattgagcaa tattgccaac tgaaacattt
     1561 tccgaatgtg acgatcaatg atatctacac cggcaacgga gtgagcgaat tgatcacgat
     1621 gtgtatgcag gggttgctgg acaatggaga tgaagtactg gttccaatgc cggactatcc
     1681 gttgtggacg gcttctattg cattagctgg aggcacacct gttcattata tttgcgatga
     1741 agaggcagaa tggtatcctg atattgatga tatcaaatca aaaataacga gtcggacaaa
     1801 agctattgtt atcattaatc ctaataatcc tactggggca ctttatccaa aagaattatt
     1861 agaagaaatc gttgaagtag ctcgacaaaa taacctgatt atttattccg acgaaatcta
     1921 tgatcgctta gtgatggatg gtctagaaca tattccgatt gctacattag cgcctgattt
     1981 gttcgttgtt acgctgaacg gtctatccaa atctcatcgt gtagcaggat ttcgagtagg
     2041 ctggatggtc ctaagtggag ataagtcaca cgtcaaaggc tatatagaag ggctgaatat
     2101 gttgtcctcc atgcgtttgt gttccaatgt attgtctcag caaattatcc aaaccgcgct
     2161 aggcggttat caaagtgtcg atgagctgct cctgccaggc ggaaggatct atgaacaaag
     2221 agaatatatc tataatgcaa tcaatgatat tcctggtctt tcagccgtaa aaccaaaagc
     2281 tgctttttat atattcccaa aaattgatac aaagcgattc aatattttgg atgatgaaaa
     2341 attcgttttg gactttttac atgaacatca tattctactt gtacatggtg gtggatttaa
     2401 ttggaaccag ccagatcatt ttcgaatcgt ttatcttcct aaaatggatg atttgaaaca
     2461 aacagctaag aaaataagag aattcctagc aacctatcgc caaaaatgat tttaaaaacc
     2521 aaatttttca gtaggagggc cacagacaaa cacactgagg ctctctttct agtggagttt
     2581 tcttaaataa ctaaagtata ttatttagtt gcaagtcttt tcttgaagtt tgcgacttcc
     2641 taaagtaaaa tacgaataga atgcgaatac gcatattttg gggaggatac ttatggaaac
     2701 gttaatcgag tcctttggaa cgatcattaa agagattcga aaagaacaaa aaatgacgca
     2761 aaaaatgctt tcgcatgata tttgttcaca aagtgtatta agtcggatcg aaaataatga
     2821 ggaactgccg aatgtattgg tgatgatgca gatttgtcaa cggctagggg taacgattga
     2881 tcatgtgatg aatctctctc gaaaaactac tcggacaaac gataaattat ttgaactgtt
     2941 ggatagttgt ttccaaaaac gtgattatcg gaagttagaa caagtactaa aatcttctga
     3001 tatttctgaa aatctgtatc aagcaactga ctttcaacgc tattattatt atctcggtgt
     3061 ttgcgaattc acgttgcgtc aaaatatctc tcagtccctt tattatttga aagaagcatt
     3121 aagctactct agccaaaaag aaagaatcca tgtttctgac acagaaatac aactgattag
     3181 ttgtatcgga aaaatatatg gtgtggcagg aaaaacggct gaagcacgct actatttgag
     3241 tcgaagcatc caactgctgc atcaatcagc tgatgaacga tcaaaaagtg aattttcgca
     3301 aattttttat aattatggaa atttcttatt ccatcaaaat gaaatcgacg aagcgttaga
     3361 acaggtgaat caaggaatct attgggctca agagaagaat agctactatt acctcaatga
     3421 tttatttgtg cttaaaagct taatatacaa gcgtaaagaa gaaccagaaa gagcagcatt
     3481 ctatgaaggc ttagcacaag ctgtgaaaca aatttcaaaa aatttgtaaa tggtgtagac
     3541 cattcttgac ttcttctcta aataagagta ctttagaggt agaaaaacaa agggagagaa
     3601 tagaatgaga acttttatct atgctgacaa attcttttta gcgtcaggtg tcaaacatgc
     3661 aggctatctt gaaattaccg atggactttt tggtgaatac accaaagaac tacctgaggg
     3721 agaagtcaac atcattgacc aaagcggcaa atggattgct cctggtttgg tagatacaca
     3781 tattcacggc tatatgaatc atgatgtaat ggataatgat gcagaaggca tcaaagtgat
     3841 gtctgaaggt ctgttatctt gtggagtgac ttcattttta ccaacaacat tgacgtcaag
     3901 caaagaacgt ttaaaagatg tagctgagac aatcggcaaa atgtatcagg aagcaccagg
     3961 agctaaaatc aaaggaatct attttgaagg tcctttcttc acagaagaac acaaaggggc
     4021 acaaaaccca agttatttcg gggatcctga tttggatact ttccatgaat ggcaagaagc
     4081 ttcaggtggt ttaatcaaaa aaattgcttt agcaccggaa cgtcaaggtg tagaagagtt
     4141 tgtcaaacaa gtgactgaag aaggagtagt tgtttctcta ggacacagta atgctacact
     4201 agaagaagcg caaaaagctg ttgaagcagg cgcaagtgta tttgtccatg ctttcaatgg
     4261 tatgcgtgga ttgaatcatc gcgaaccagg tatggtgggt gcgttattat ctttagaaca
     4321 tgtcttttct gaattgattt gtgatggcca tcatgtacat ccaaatgctg cgggaatcct
     4381 aatggaaaaa gcaggacatg atcatgtcgc attaattaca gactgtatga tggccggtgg
     4441 gatgcctgat ggaaattaca atttaggcga atttccagta gtagtaaaag aaggaacagc
     4501 acgtctagaa tctggcaact tagctggaag tatattgaaa ttgaaagaag caattaaaaa
     4561 tgttgtagac tggggattag caacaccaga acaagcagtc atgatggcta gtctgattcc
     4621 agcaatcagc tgtaaaatcg atgatacatg tggaaaaatc gctcaaggac gcgatgcaga
     4681 ctttatcgta ttgaaccctg atatgacatt ggaagcaact tatctagatg gcgtggaacg
     4741 tttccacgca taatataatt taagttaacc ttaaaactaa acttcttaag tttggtttta
     4801 aggttttttt tgtgttctag taaaaatcac tttacataag tttcaaaagt ttctttaatt
     4861 tcctattgtt caataagtaa caatgtaaag ataaaattaa aaatgttaat acgtattagc
     4921 aaaataaaag ctttttttat tgtgaataaa tgaacataat taggaggaga aagatgaact
     4981 tatttgacat ttttcgatcg actttgacag atatgtcgat tgtcagtgca atcacttcaa
     5041 cagtttttat tatattatta gggtttgttt gtagaaaaaa ggggattttt tctgaatctg
     5101 ttggaaaaat tctatcgaaa gtcgtcttaa cagtggcttt gccagcactg gcatttcgag
     5161 ctttcatgca agatatcaat acagaattac tgatgcaagg aatcaatgtc ttgatttggg
     5221 gattagttat ttatattatt cttattctag tttcaaaacc ttttttctta agctataaag
     5281 gggataagca aacaacgttg cgtgtcctga cgatctttgg ttctacgacc tttttcggaa
     5341 cgcctatcgt cagtgcgatt tatggtcctg tcggcgtgat gtattcctca attttcaata
     5401 ttggctaccg aatctttttg tattcttatg gatacataaa aatgagtggc ttgaagatgg
     5461 aagctaaaaa tatcaaagcg atgttcgtca atccaatcat tatcgctact tttgccggcc
     5521 ttttcatttg gctgttccaa gggtatctgc cacaagtgtc agttacaggt gcagaaggag
     5581 agataagcca agtagcgttt ttgcgaattg accagactgc accgtggcta tttcagccga
     5641 tggattattt agctggttta agttcaccat tggcgtggct agcaatcggc gcaacattag
     5701 gtgaagtaag cttcaaaaaa gctgcatctg aaaaaacttc atggtattac agtctagtca
     5761 aagtggtatt tgtacctgca ttgaatatcg tgattctagc agttttgacg gcaacgaata
     5821 ttctaccagt caattttgaa gcactggcaa caatcgttat catgatggct acaccagctg
     5881 caacagtggc agcagcctat gctatcagtt ttgataggga ggcactttta gcctccaatg
     5941 catccttact gtcaactatt tttgctgtgg tactgacacc tgtatggatc gttgtattac
     6001 aaatgattca tggcataggt ttattttcat aagaaatgga ggaagaaagt atgagaatcg
     6061 catgttacgg tgttcgtccg aacgagatag atttttttga aaaattgaat atttaccatt
     6121 atgatctcag tctttatgaa gaactgttga cacatgacaa tatcgtgtca gcaaaggacc
     6181 atgatgctgt attgttgagg ggaaattgta tagcagatga gaccaatctt gcaaaaatgc
     6241 aggaatatgg gattcgttat gtttttacac ggactgtggg agtcaatcat atcgatctac
     6301 aagcggcagc tgattttggt atgacagtag cccgtgtacc atcgtattcg ccaaatgcga
     6361 tagcagagtt atctttgact tttgccatga tgcttttgag aaatactgcg tatacgacga
     6421 tccgaacatc tttcaaagat tttcgagtgg atgagcaaat gtttagtcgt gaaatcagaa
     6481 attgcacagt agggatcata ggaacaggca ggatagggtt gacggaagct gttttgttta
     6541 agggactagg agccaaagtg atcggctacg atattcatca gacagaagct gctaaaaaag
     6601 tattgacgtt ccactcgctt gaagagttat tagccaaaag cgatattgtc agtattcatg
     6661 tacctcatat cccaggtgaa aacgatcaaa tgatcaatga aggattttta gctcaaatga
     6721 aaaaaggtag catcttgatt aatacagccc gtggagagct tcaagataat gaagctattt
     6781 taagagcttt gaaaagcaat catttagctg gttttgcaac cgatgttttt gcaaatgaaa
     6841 cagaggtttt tttccgatcc ttcaagccat gggagacgat tccagaccct gctatccagc
     6901 aattagtaga aatgtaccct agagttcttg taaccccgca tgtaggttca aatacagatg
     6961 aggcattatc aaatatgatt tcgacaagtt tcgagaactt tcgtgaaata atagaaactg
     7021 gaaaaacgaa aaatgaggta tctcttccta aagcccgtca attaagataa gaaaacatgg
     7081 atattcgtca ataaaatgtt taaaaatagt ccaagtaaac agttgttact tctgtatact
     7141 tggactattt tgtatgggat aattcgatga ctcaacagta atctgtgagc tttttccgcc
     7201 cattctttgt caattcataa caggttagcg gccggccgat ttccaaatat ttgattcttt
     7261 cgttcaatat atcttgttct actaaaaaat tcaaatattt cttgacggat atacgtgaca
     7321 gtgagctttt ttgagccagt tcttttgttg aaaaaatagt atcttctttt agaataaatg
     7381 cacttatttt ttttaaagtc agttttgaca gacctttagg caaatcgatg aatgatggta
     7441 tttcgtgaat gggagtggtg tggttgaata cttgatcgat caatttctga tcggcttttt
     7501 cagtcgtttg gattgtctga gagaaagcaa gatacttttc gatcgccagc tgaaaacgtt
     7561 gcaaagtgaa cggtttgatc aaataatcta aggcaccgta gcggatcaca ttctttatgg
     7621 tttcgatatc atctgcagca gtgatcaaaa tcactgggat atcatttcct tgctgtctaa
     7681 gccaagataa aaaatggata ccactttcgc caggcatgta tacatcaagc agcagcagat
     7741 ctgcgttttt ttgtctgaaa tatttttttc cttcttctac atttctgaaa ctttcgatta
     7801 cgttcatatt agggatgctt tccacaaaag tacgattcaa ttctgcaacc attggatcat
     7861 cttcgattat caggacattt gtcatcattt tgatcttcct tttttggtaa ttctagatag
     7921 acgattgtac cgccatttgt ttgtttggaa aattctaaaa atccaccgtg gcgtttgacg
     7981 attgaatgga tgagattcaa tccaaaccca cgattgattc ccttcgtcga aaaacctagc
     8041 gtaaatattt tttctgagat ttcttctgat atcccgacac cgtcatcgca gacggtcacg
     8101 atgaatactt cagcttctgt gtcatagttt aaatgaatcc ggacctgtcc gtttctttca
     8161 tgtttttgta gcacggcttc ttttgcatta tcaagtaagt tccctaacac ttgcaaaagt
     8221 gaatggatgg agtcatccat ctccatttta gggataaaag aatcgctttc taacagcatt
     8281 tcaacagatt gttctttggc ttcgtttatt ttgcctaata aaaaaccggc aattgcaggt
     8341 actttgatca gacttgagac ttggccaatc tcttttttat aattttgatt tagctgctgg
     8401 ataaaatggg tgacttcttc atacttcttc aaatcaatca aaccagaaat gacatgaagt
     8461 ttattcatga attcatgggt ttgagctctc atggagttta tatattgttt cgtcccccca
     8521 agctcctcca ttaatttcat tacttctgat cgatttcgta aagttgcgac tgctcctaaa
     8581 aaacggtcat tgctaaaaag aggtgtaacg ctagaaatag tttctatccc atttaaatac
     8641 atggattgag tccgaacggt cgtttgatgc tcaatcgtct ctttaaacaa aacgtcatat
     8701 agattatcac tgattttttt cctacttcga aatccaatag ctgctctgtt tggtagaaaa
     8761 tgttttttgc aggtgtattg atcaagagga tagttttatc agaagagatg gctaagattc
     8821 cttcatctac ttcattttcg ataatcgttt tttcacttaa ctgttctgct atttcttgtg
     8881 gttcaagtcc aagcagtact tttttcattt tttgggcaag aaaaacggcg cctatgatgc
     8941 ctacgctaag tccaataatc aatgaaaaaa caatcatttt ttgtccatct ttgacttgat
     9001 tgtcaatcgt gttgtttgtt aagccaacac aaactgcacc aataatctct tgatcataat
     9061 aaatagggac gaaatagcgg aagccgaacc ctaacattcc ttctttttca gagaaaaatt
     9121 cttgtcctcc aatcactttt ttcacatctt catgacttga gaaaggaaga ccgatgtaac
     9181 ttgggtctgc gtgagaatat cgaatgcctt cagggtccat gacaacgata aaatctaaat
     9241 tcgatgcaat catgatttca tttgttattc cttgaatcgc atgtgtcggg tcaccagtct
     9301 gcagcttttt ttggatgagt ttatcattgg caacgatttt agcaatgcta gagattttat
     9361 ctgacacact agatcggttc atcgaaagaa taaaattcct gatcaatatg gcagaaacaa
     9421 tcaaagaaac gaatgtggag aaacaaacga caaggatgat cgtgttctgg agctttaatg
     9481 gttttttctt aaaaaaagga aacataggct acctcgaaag aatttattgt gaaaatttta
     9541 acatgaaaac aaaaatttaa caaattgtca acgataaaca caaccttcct ataaaaaaca
     9601 ccactgagtc ggtaaaagaa gctatacaga attttcaaac attggtatga tagacgtaga
     9661 aatggaagga ggcgtgtata tgataggggg aatcgcactt gagacgattt attggtatac
     9721 attgctgatt tgtgcaggat tggcagtcct attgattatt tttggagatg tattcgattt
     9781 tgatggcccg cttgatccga tgcttattat cccttggtta actttcacaa gcttatttgg
     9841 ttttctaggt gagcgtttaa cagatcaatc atctttattt ttatttattg ctagtggagg
     9901 catatcgata gtgttggtat ttttgcttaa tttctatata ttgatgccga tgaaacatgc
     9961 cgaatcaacg atgtcgtctt ctgaaaaaac tctggaagga caagtagctg ttgtgatcac
    10021 accgatccct gtacgaggta tgggagaaat tcagttgaaa agtgttaccg gttccattag
    10081 tcgtccagct tgtttttaca gcccccaaga acagacagct gagcggggaa cgaaagtatt
    10141 gatcattgag atcaaggaaa gaatctgtta tgtcatgcct tatgaaagca tgataaaagc
    10201 ttaaaggagc gtgtgttaaa tggaaatacc agttgttttt atcgcgatcg caatcgcagc
    10261 tttgatttta ctgatgttgc tgatcgtctt tgtagcgaag taccagacag caaaaccgga
    10321 tgaagcgttg atcatcagtg gaagttattt aggaagcaaa aatgtgcaca ttgatgaaag
    10381 caataacaaa atcaaaattg tgcgtggagg tggcgcattc gtcttgccag tttttcaacg
    10441 ttcgaatcgg atcagtctat tatcgagtaa attggacgtc tccacccctg aagtgtatac
    10501 ggaacaaggc gtaccggtga tgtgtgatgg tacttcgatc atcaaaatcg gttcatctgt
    10561 agaagagatt gctacagcag cagaacagtt tttaggcaag acaagagaag aactggaaaa
    10621 tgaagcaaga gaagtattag aaggacactt gcgctcgatt cttggttcta tgactgtaga
    10681 agaaatctat caaaatcgcg ataagttcag tcaaagcgta caagaagtcg cttctgtaga
    10741 ccttgcaaaa atggggttga tcatcgtttc tttcactatc aaagaggtca gagacaaaaa
    10801 cggttatctt gactctttag ggaaaccaag aatcgcccaa gtcaaaagag atgcagatat
    10861 agcagaagcg gaagctctaa aagaaacgcg tatcaaaaaa gctcaagcag aaaaagaatc
    10921 acaagcagct gaattacagc gacaaacaga gatcgcagaa tcattaaaag aaaaagaact
    10981 gaaattagca acctacaaac aagaacaaga tgttgctaaa gcaaaagcag accaagccta
    11041 caatttagaa agtgcccgag cacaacaaca agtaatcgaa caggaaatgc agattaaaat
    11101 cgtagaacgt caaaaacaaa tcgaactgga agaaaaagaa atcacacgtc gtgaaaagca
    11161 atatgattcg gaagtgaaga aaaaagcaga tgccgatcgg tacgctaaag aacaagaagc
    11221 acaagctcaa aaagtcaaag aagtgactga agccgaagca gaacgattcc gtgtggaagc
    11281 actggcagaa gcggaagcca ataagacacg tttagctgga caagcagaag ccgaagcagc
    11341 actagcaaaa ggaaaagcag aagccgaagc aaaacaaaag atcgccaatg ccttcaagga
    11401 atatggcgaa gcagctgtct tgagtatggt tatcgatatg ctaccgcaat tgatgcgcga
    11461 agcagcacag ccgctaggaa atatcgaaaa aatctctgtt gtcgatacag gaagcagttc
    11521 aggagaaacc agtggagcga accgagtgac aaattatgcc acaaatttgc tttcttctac
    11581 tcaagaaaca ctaaaagcga caacaggact tgacttgaag gaaatcgtag agaatttgtc
    11641 gacacgtggg actactagcc aagtaaatat gtatgaagcg ccaaaagagg cagaacaaaa
    11701 ctcgatgaag gaagaataaa gtagtacact tcaccgttca atcaaaaatg gaggaagact
    11761 ttttaaagtt cttctctcca tttttttatt ttattgcaaa agaaaattag tcctcgctat
    11821 ttttcttttg tttttggatc ttagcttcta tctcttgtaa atttgttctt agcaatcgtt
    11881 ctttcttttg gagttcgacc aaatcttctg atagttcttc cttcttttct gtttcaggag
    11941 gacgaagaaa gaaattgata ccgattgcaa tgaatgtccc tataaaagta tctagtacac
    12001 gttggatagc atacaaagcg gattcgcctt gagggatgct caacgcaatc agcaacaacg
    12061 tagctattgc ggaaatgata ccggaattat tgttaatacc atctgacacg acgatcacaa
    12121 taataactag aataggaagc aagatcaatt ctaccaaaaa atcattgtga aaatagcttt
    12181 tgacaaaaaa ataaatgata gctaatgtac cacctaatgt attgccgatg atcctagact
    12241 ttccaaaaga aacggttgta gtcaagtcct gacgcaaaga aaaaacagca gataatgcag
    12301 caatcatcgg gattcctcgt tgagtcaaat gaaaaaacaa gatacataaa aaaacagcta
    12361 aggcactttt gatcgtacgc atacctagtc gaaaacggcc aaattgcata gagattctcc
    12421 tttttggtct ttttactatc atacctaaac ttttgtctga tagctagttt ttcattattc
    12481 aagtataatt aaaataaaaa agggggaagt aaatgcgtat aggaatgcga acagtcaaaa
    12541 cagtgatcag tgtcttcttt tcgatggtca cagcgggtag cttatcgctg ttgtattggc
    12601 cagctgcagg gatcattgca ttattaagtg taggaaatac caagcgctct acattgcgta
    12661 cgggcatgaa tcgaatcgca gcctttatat tagctacggc aacagcactg ttcagttttt
    12721 ttgctgttgg gtatactatt tttggcttca gtcttttttt attcctgttc atcccgcttg
    12781 cctctcgctt caaattaacc gaagggatag tagtaaattc ggttttggtc actcattatt
    12841 tagtggagga aaacatgtct tggcagctga tcggcaatga agcgggatta atggcgattg
    12901 gattagtatt tgctttgctt gcaaatgttt acatgccaga tcgtacgaag cagctgaagg
    12961 aaaaccaaat acagatcgaa aaagaatttc gaaatatttt gagacaaatg gcagaatttc
    13021 ttttatcaga aaataaagaa gatgtgcaga tcaaatgcga acatttactg acattcattc
    13081 gcgaaagcca agaagatgcc agagaacatc aagaaaacta ctggctcaga cagccgctat
    13141 actacgaaac ttacttctct atgagaagag cacaggccaa tgtgattaaa gacatgctgg
    13201 aaaatctaga gagaatccag caaccagctt attatggaaa acatatctat ggtttattga
    13261 tttatactgc agaaacattt tcagagtcaa atgatggcag acaaatttta acacgtattg
    13321 aagaagttta tgtattgtat cggcaaatgc ctctgccgac tagtcgccct gaattcgagg
    13381 atcgagcaga attgttccag tttttgcagt cttttaaaag ttttattgaa atcaaggcag
    13441 aattctccca acaaaaaatc caaaaataga ggttgtgaat tcagctaata ttcaggtcaa
    13501 aaagctgatt tcacaacctc ttctttaaac ggtgttctcc tgtcaactcc tcggtcacaa
    13561 gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcggag
    13621 ctgaacggta ggctcacaac ctctttctta aacggtgttc aaaagctttc ctctgcggta
    13681 ataagtccag ccaaacgaaa aataggagga gctatttttg tttggctgtt cttattactt
    13741 gatgcagaac agctttttca caacctttta aaattacata tttttccata gaacttgcat
    13801 ttgatccaag atttcgatga catcttttgt caagaagaga gagcgattgg gcaagtcacc
    13861 ttcaatcatg tgtaccatat tctttatttc gtaattcatt gcttcagatg tattcccgct
    13921 ttcgatgaat tcctttgtac catctgtaaa taagatctcc gcacgatcag cacgcgggta
    13981 atcagcaatc gtcacataac cgttctcaaa ggcaacgata ccgacttttg gcatttttgc
    14041 ctgaaaagtc aagctgacag cagcaagttc attttctttg ttacgcaaga tagtgacgga
    14101 ttgttcgtcc acgcctgttt caaaaggaac cattgtagag gcaactactt caggttgaga
    14161 gctcaaaaag aaacgagcga aagatacagc atacgtaccg atatccaaca gagctcctcc
    14221 agctaattct ggattgaaga accggttttt agggtctggt tctttgtaac tgccgaaagg
    14281 tgcttggatc atttttaaag cccccagttt tcctgtatcc attattgagc gtaactgctg
    14341 ataaagtggc atgttgaaga ttgtcatcgc ttctgctaag atcaaattct tttcttctgc
    14401 taagcgcata gcgtcagcca gttctttttt attcatcgtg atggcttttt cacatagaac
    14461 gtgtttgcct gcttccaatg ctgccaagat atggtcgata tgctgtctat ttggtacagc
    14521 aatgtacacg atgtcgattt cagcatcgtc taacatttct tgatagcttc cgtaagcttt
    14581 tggaatattg taacggtgag cgaagttttc cgctttttct gaagttctgg aagcaactgc
    14641 tgttagttcg ctcgtctctt gattgaagct ttcggcaaac tcatgtgcga tggtaccaag
    14701 accaacgatt ccccattgat attttttcat ctgaaaacgc tccttcatag ttggattgag
    14761 tttcttgtct gtctattatt ataacaaaaa tcacgaagaa taaagaggag aggacttgaa
    14821 acattaaaaa aaatgaaagc agcgatttaa gaggtgcttt ttgatcaaaa cttaagtaga
    14881 atagttaaaa ggaggggata aacgtgcatc aagtatatat tgatcttatt atgagtgtat
    14941 tattagaaca gtttgaaact gaaaaaaatt ttttagatga atacttgctg atcagtccag
    15001 aacaatggca aaaatggaaa gatggcgaga aaaatctgcc atctgaagcg atgcagaaag
    15061 taaaaaatct tttcactgat tatgaatgga tgctaacgca aaagattttg cgtcaaacga
    15121 ttatttttcc cgaaaaacga aacgttgccg ttgcagagta caagcaaatc aaaacacgca
    15181 tcgcgcaaaa atggcttagc agcaaagcag caaaagtcga gctgatccct ttgaaaaata
    15241 atcagcaggc tggtagatac cttgacttga aagtaagtat tcaatatgat gaatggggct
    15301 atgatgatat tttgaatttt cgtctgccag cagatgttca aaaacagatc gaaggagaaa
    15361 aaatcgaact tctcgattgg gtgaatgaaa atctggaaga aacttacgtt catgaggcga
    15421 atggggaacg tgaagaacaa cgaaaggatt tttcaagtga ataaattttt aggatttgga
    15481 gcagttgtga ttatgatcgc agcaatgggc tatgttattt ttagcgatga tgattcgcat
    15541 gttcaagccg gcaatcaagc agtcgaacga acaagcacca aaacaactgc tagtaaagaa
    15601 aagaacagtc aaacaaccag cagcaaggta aaggacaaag aaacaaaaga tcgtacgtcc
    15661 aaacaattgc cagatgttaa aacagacgat tggaagctag ttttggttgg tccagatcac
    15721 aaattagaaa aagaaattga tgagtctact caattggcat cattaagtaa cggatacttg
    15781 atcgacaaac gcattgctcc agaatatgaa gcatttgcca aggctgctga agcagctgga
    15841 tttccattag tgatggtctc agcttaccgg tctgttatca tcccaacaac aagttttcga
    15901 acaaaatgtc caagatgtga tgagtcgtca aggtgtatca gaagaggagg cgacaaagat
    15961 tactaaagaa acgattacgg agccaggata cagcgaacac cacacaggtc tggcagtcga
    16021 tgtcgtcgat caaaactggt acaacagtta tccttctact gtattggatg caagttacgg
    16081 agatcagcct ggagctaaat ggatcgcaga taatgctgcg cagtatggat ttatcgttcg
    16141 ttatccaaaa ggacacgaag atataactaa gatcacatat gaaccttggc acttacgtta
    16201 tgtcggtaaa gaaaacgctg agtatatcac caaacaccaa ttgacgctag aagaatatct
    16261 gaatgaatta aatgaaaagt aggaattgat catggcgaaa aaaaagtgga aaaagcaaaa
    16321 gcgcaagaat atccgttggc cagcagtggt tgccggtgta gcagtgatac tcttagcctt
    16381 tgttttttcc ttgaagaatc tgtcttctcc ttttactgat aatcagcaag aaaatgaagc
    16441 gcaatcccat cagcaattta ttgaacggct tgttccgcat gcccaagaac tacaagatgg
    16501 ttatggagtg cttcctagta tcattttagg acaagctata ttagaatcaa attggggaaa
    16561 aagtaccttg gctagcaaat acaataatct tttcgggatc aaggcttatg gggatcagaa
    16621 gaaagtaagc ctggaaacaa aggaatttgt taacgaagaa tggataacta tccaaggaga
    16681 cttcaaagtg tatgattcat gggaacagtc catggatgac catacacaac tgttcgtcca
    16741 aggagtagat tggaatcctg cgctgtatga aaaagtgatt acggccacca actatcagga
    16801 agccgcacaa gcattgcaag atgcaggtta tgcgacagac cccggttatg cgcaaaaaat
    16861 catccaggtc atcgagacgt atcaattgaa tcagtatgac cggagatagc aaaggagaag
    16921 tggctgatgg cagaacaatt tacgccagaa atcatgacgg aattgagaaa agatatcgta
    16981 agagtaccgg aaatcatttt agaagcaagc gggatcaaga tatttggaaa attgatccgt
    17041 tcgatcatct ttactacgga tattgcgatc atacgtaata caaatgcgaa tgcagtaatt
    17101 gctgtgtatc cttttacacc tcatccagcg attactaaaa gcattattga ggcagctgac
    17161 attccagttt tctccggtgt aggtggtggt ttgacacaag gtgctcgttc ttcctacatg
    17221 agcatgttcg cagaagcgca aggttcgtta ggggtcgttt tgaacggtcc tacgccttta
    17281 gagacagtaa aagaagtttg tcgagtagtt gatattccag ttatcagtac ggtaacgtcg
    17341 aaatatacag aaatagatga aaagttagca gctggtattt cgattatcaa tatcagtgca
    17401 ggtaaagaaa ctgcagaagc agtacgccat tttcgtaaaa aatatcctga acttcctatc
    17461 attgcgacag gtggtccaac ggaagaaagt attgctgaga ccattcaagc aggagcaaat
    17521 gccatcacat atacgccacc aagtagcgga gagcttttta gtcgtaaaat ggaaaaatat
    17581 cgggaaactg agaaaaagaa tcatgaataa caggtgagaa tcaaaagacg aggaagagca
    17641 gatagtggct cttcctcgtc ttttgtactc cagtactatt tgttcagaat tcaaaaaaaa
    17701 gagaaatttt tttgtttttt tacctgtaat gtacataaga agagttaaaa ccttttgtta
    17761 ttattagcat tctaacaaaa aaatgtcttg atttattgaa gcgctttcta tagaatgaaa
    17821 aacgaataaa aactaaaaaa ggcacccgac aatttagtag cagctaaatt gtcggggctt
    17881 gaaaagtcag cgggaactga ccaatcaagt tgccatagtc aatgcttgtg catcgacgtg
    17941 tatattgtac ctaatttcat acagaattac tagttatttt tctgtttttt agggaagagc
    18001 gcacacgaag aagcatgctt atttagctat gtcaaggata ttgggcagtt gtctgatatc
    18061 cttttttctt tgcgtaatta tcctcccatc attgaagcaa gaagtgtttt tagcgatgaa
    18121 agaagaatca gcactatcta atttttcttg aaagtattga gttgttcatc ctaggcagaa
    18181 cgataaggca agtcttggtt tttattcccc ttgaaaaaga tcgtttaata aatatgttct
    18241 gtataaaaat acagtatatt gaaaaataac aatggaagta gaggtgcaga gaatgaagaa
    18301 aagaatgaga atcatcttag ctttaagtgt tatgttattc ttcttgacgg cttgtgggca
    18361 agaagatgac aaaaaaatca cgctgaacat ggcttggtgg ggttcgcaag tccgacacga
    18421 tgcaacagta aaagtaatcg agctttatga agaaaaaaat ccccatgtga cgatcgatta
    18481 tgaattttat gattatgaag gatattacac aaaattaggt acactagcag catcagatga
    18541 tatctgggac attttccaac tgaacaatca atttcctcaa tacatcaatc agattgaacc
    18601 attagatgaa tatgtggagc aagggataat tgatatttct aatgcagatg aatcctattt
    18661 agcaacgacg acttacgacg ggcaattagt tggactgtct aacggggtga attcgtttgc
    18721 gattgcttac aataaaaata tatttgaaca attaaatatc cctgaaccag cttcaaattg
    18781 gacatgggat gaatttgaac aaatcagcta tcaaatcact gaagaattag gaatctatgc
    18841 tatttcaaga ttcgaagatt ttatagctgg ctgtattatt caaataccac aagttgagaa
    18901 aggattgaat ttttataatc gggaagatct atctaacagc ttgggatttg aaaatccaga
    18961 ctatctaaca gattttttcc agctgcgtaa aaatctggta gacaaagggg cccatccaga
    19021 accaggtggt ggagctgcaa gtacgacgga tctgtctttt caggcggtta gggatagaga
    19081 agcggcaatg gtgtttctat caagtaacca gttgacagaa atattgtcag gggctcctga
    19141 agatatggag attcagttga ttaatccacc tcgcagaaaa gcagatggag aatctggtat
    19201 ccctttacgt tcttcgcaaa tgctgagtat ggcaaaaagc tcagcccata aagaagaagc
    19261 tgctaagttt attgatttct ttcaaaacag tgaagaagcc aatgaaattt taaaaggaga
    19321 acgtggtgta ccaatcatgt cgaatattcg agaacagctc gctcgtgcag atgatgcaac
    19381 tttgcaagcg acatttgatt atttagaaat gatttccgat atggacaacg gcgaagtgaa
    19441 tgtctttgat agtgctttga atccggaaat tcaagaccaa tacaatcttt tagttgaaaa
    19501 agtcatttat gaagaagtaa cagcagaaga agcagcagag agtctttatg agtttgcgtc
    19561 aaacattcta ggatagcagc aggaggaagg catgaaaaaa gaaaaactaa aacggttctt
    19621 ggatcaagac aatgtggtcg gttacgtatt tgcttctcca ttcatcctag gatttctatt
    19681 gataacagtg ataccgatga tcttgtcact ttattattcg ttcaccgatt atcgtttggg
    19741 aaatccagtc tcatggatcg gcttggataa ttatataaag atgctgagtg atgcccgttt
    19801 tttgaactcc atccgagtca cttttctcta tgtatttgca tctgtacctg tgaaactgat
    19861 ctttggtcta ttgattgctt atcttttaac gaggaatatc aaaggaacga ctttctttcg
    19921 ttctttgtat tatgtgccta gtttgatcgg tggcagtatt tctatcgctc tagtttggcg
    19981 ggagttgttt tctaaaaacg ggttagtgaa tattttcctg gaacgattag gcgcacctgc
    20041 gctttcctgg tttagcgatc aaaaactggt catgatccct cttgttttga tgtctgcttg
    20101 gcaattcggt tcggcaatga tcatctttgc tgcaggtatc aaagagatcc caggctcgta
    20161 ttatgaagcg gcagagatcg atggagcgaa caaatggcag acgttttaca agatcacgtt
    20221 acctcttctt tcgccgatca ttttgtataa cttggttatg cagacaatcg cggcgtttat
    20281 gacttttacg caggcattta tcattaccca aggtggtccg aatgatgcca cgaactttta
    20341 tgctctttat gtgtataacc aagctttttc ttttaattct atggggtatg ctagtgctat
    20401 ttcgtggatc atgctgttga ttatgggagc tgtcaccttt ctgattttca aatcatcaaa
    20461 aaaatgggtc gttgtgtgaa tgagaggagg aagaaatgaa taatacagta acgaaatggt
    20521 ttcaatacgg ttttattttg ctattaggtc tagtcatgat ttatccggtc ttatggatga
    20581 tcgcaggttc ttttaaaacg gatcaagaga ttatgagtgg ttcgctgaat ttgattccag
    20641 agacttttca atgggaaaat tacgcaaaag gatgggcggg gtttgctgga atttcatttt
    20701 ttactttctt taggaattcg tttgtcatct ctgcagtttc gacgataggg acagttctga
    20761 gctcgacttg tattgcttac gcattatcga gaatcgattt caaaggaaaa agattctggt
    20821 tcgtcgtcat gttagtaacg atgatgattc ctgcacaagt gattttgatt ccgcagttta
    20881 ttatttacaa tcggctgaat ttggttggga cgtatgtgcc gctgatttta ccacattttt
    20941 ttggacaagc atttttcatt taccagatca tgcaattcat ggtcagcatc cccaaagagt
    21001 tagatgaatc agcgattatt gacggatgca gcaagtatag tgtctttacc aaaattattt
    21061 ttcctttgct gaaaccttcg atcatcacga cgatcatcat ccagttttat tggaaatggg
    21121 acgacttcat gggaccattg atttatttaa acaagccgag atcttacacg gtatcgattg
    21181 ccatcaaatt atttgcagac gctggatcta gtacgactta cgcgagtatg tttgcgatgt
    21241 ctacgttgtc gctgctaccg gtttttctga ttttcttatt cttcaataaa tatcttgtcc
    21301 aggggatcag tacaagcggt ctgaagggct gaatatgaaa aagccaatag aagagatgtt
    21361 tctttacaag ctcaactact atgtctgtcg gattttttta accagtctta tcagtaatgc
    21421 tcttttcgct ttagttctag gatgtttgtt cactttagga ttagtgctgt ctgctcgtgt
    21481 tgtctatctg atttattttt tgatgatgta tccttcttat tatctatctg tccattactt
    21541 ggcagaaaaa aaggtatgtt ggaggaaagg aaaaatcaaa cggatgtgtt tcatcattac
    21601 gttgtttcag ctgtttgtca ttatttttgc agttgattgg tatttattag tgggaaagat
    21661 tggagcttgg tatctgattc cttttctatt gtgtctgttg ctttttgtat cccatagttt
    21721 ttttagtacg ctaattgata tctgcaaaga agataaagca gatagtcttg tttatttgat
    21781 caaaaggaat atctggctgt tgtcaaaaaa cgtcaagggg tttctgtggt atttatgcct
    21841 tttgtatgga ctgttctggt gtatacagca tgatttttta ctcactgcat tactgtatcc
    21901 agggctgcca tttgtcacag tattttgctt aaaagaaaag gagaatgact ttaaatgaag
    21961 aaaaaatatg tacaagtcgg acttggtggg agagcgcgtt ttttttatga ggcaattgcc
    22021 tctacgtatc atcaaacttg tgaactagtc ggtttttgtg atgtatctca aacaaggatg
    22081 gattatgcaa atcggatact tgtgaacaag tatggacaga aaaaagtaaa gacatgtatg
    22141 ccatcacaat ttgagcaact actggatgac gtacggccgg ctgaagtcat tattacgtca
    22201 gtagatcgaa cgcatcatga ttatatcatt cgtgcaatga aaaaaggata tgatgttatt
    22261 tgcgaaaaac ctctgactat cgatgaggaa aaagctcaag aaataataac ggtccaaaaa
    22321 cagacgggaa gaagcctgcg agtcactttt aattatcgtt atgcaccgta tcacacgaag
    22381 ataaaggaac tgatagaaag tgatgtgatt ggtgatgtgt tttctatcca cttcgaatgg
    22441 ctgttaaata cgcaacacgg ggcagattat ttcagaagat ggcatcgaaa taagcacaat
    22501 agcggcgggc ttcttgttca taaatctacc catcattttg atctagtgaa tttttggttg
    22561 aacagccgtc cccaaaaggt atttgcattc ggtggattaa acttttatgg gatgcataat
    22621 gcggagaaac gcggagtaga aatattttac agtcgatgct atggaagcga actagctaaa
    22681 gctgatccat ttgctataga cctgtcagcc aatgaggaat tgcgagaaat gtatttagaa
    22741 gcagaaaaag acagtggata tattcgtgac cgcagtgtat tttccgatga tatctctatt
    22801 gaagatacac taggcgtaac ggtcaaatat gaaactggtg cgattttgac gtattcgcta
    22861 aacgcgtata tgccgtggga aggattcaat gtttctttca atggcagcaa aggacggatc
    22921 gaaatgaaag tgatcgaaaa gtcttacatc aatgcaggtg gagataaaaa ggatgaagga
    22981 gcagccgttc ttcagtccat ccatgtctat ccgatgttcg gtgagccata tgaggtaatc
    23041 gtcgaacaag aagcgggcgg acatggcgga ggagatccgc gtttattaga tgatctattt
    23101 ggtgaagaaa aagaagaaga tccgtggaat cgggcagcaa ctcacgtaga tggtattttg
    23161 tcgattttga caggaatcgc agcaaatcat tctattgctt caggaaaagc agtagtgatt
    23221 gacgagcttg tcagcttaaa agaataagaa tagatttacg gaaattactt cccgtagttt
    23281 ataaaatctt gccttattgc cgataggctg tcttttgaac actatttatt cggaaaaagg
    23341 ggctatgaca tgctttttgt catgccttct ttttatgtgg gctgcttttt tagataggag
    23401 aaaataaaaa taactattta aacccgaaca aaaaggaata atgcattaaa aatcattaga
    23461 ttttacaaaa aaataagtaa accacgttgt cagtttcaga aagtagtgtt agactgtttt
    23521 caatatttga aacaacattt tttattcaaa tatggcaaac tgccttcacg tgcaaaacag
    23581 ttgcaagaaa atgaatttac actattatta gttttacata cctacttata tatcgtcata
    23641 atatggatga cagtttctag ccagtaccgt aaatgctgga ctataagtaa aagattggct
    23701 atttcaccct tcttttctta tgataaagtg gagggtgttt ttctttaaca gaaacatagt
    23761 aggtttttgc acaaaagcgg ttatttcggt attaataaat gataaaggag caaataaagt
    23821 gaaagaatta gttgaacgta ttcaaaaaga cggacgtata ttaggcgagg gcgtattaaa
    23881 agtcgacagc ttcgtcactc accaagtaga ccctgtattg atggaacaaa tgggaaaacg
    23941 gtttgcagaa gtatttaaag aacaaggaat cacgaaagtt gtgaccattg aagcttctgg
    24001 gatcgcacca gctttatttg ctgcaagaga attagatgtt ccaatgatct tcgctagaaa
    24061 agcaaaaagt ttgacgatgg atgaagaatt attgacagct tctgtttatt cttttacgaa
    24121 acaagtgaca agtacgattt ctatttctcg taaattttta tcttcagaag acaaagtatt
    24181 gattatcgac gatttcttgg caaacggaca agcggcaaaa ggattgatcg aattatgcca
    24241 gcaagctgga gcgcaagtag aaggaatcgg gatcgtcatc gaaaagtctt tccaagatgg
    24301 acgtcagtta cttgaagaaa tgggattgag agtcgtttct cttgcgcgta ttgcttcctt
    24361 gacgaatgga caagttgagt ttcttgagga ggacgcataa tcgtggaaaa agaaacacaa
    24421 aatggaaaag ctgcggtttt agggctgcag catctgctag cgatgtatgc aggagcagta
    24481 gcggtgcctc ttttgatcgg tacaggacta ggctttaatc aagaacagat gacttatttg
    24541 atttcaattg atatctttat gtgtggatta gcgacattat tgcaattgac agttaaccga
    24601 tttttcggaa ttggcttgcc tgtcgtttta ggatgtgcga tccaagcagt agctccactg
    24661 atcttgatcg gaaccaatga aggagtagga gctatctacg gatctattat cgcttctggg
    24721 atctttgttg tgttagtttc tggtgtcttt tctaaaatta aaaaactatt ccctcctata
    24781 gtaacaggaa cggttatcac cgttattggt ttgactttga ttcctgtagc aatcgagaaa
    24841 atgggtggag gtaatgcaac agcagctgat tttggtgaca aaacaaatct gctacttgca
    24901 tttgtgacga ttctcctaat tgtaggcgtg caaatgctgg caaaagggtt cattcgttca
    24961 atcgcggtat tgattggttt agtcggagga agcatcttag ctgctttcat gggaatgatt
    25021 gatctgagcg cgattggaga tgcgccgttg ttccatgttc cacaaccctt ttattttggt
    25081 aaaccaactt tcgacgtatg gtcgatttta ctaatgatca tcatttccat ggtcagcatg
    25141 gtagaatcaa caggtgtata ttttgcactt ggtgacatta ctgggaaaaa tgttggagaa
    25201 gaagaattga aaaagggata tcgtgcagaa ggacttgccg ttatcttagg cggtattttc
    25261 aatacattcc cttatactgg cttttctcaa aatgtcggat tggttcaact ttcgggaatc
    25321 aaaacaagaa aacccatcta tttctctgca ttcttcttga ttgttttagg attattccct
    25381 aaaatcggtg cgttagcaca aatcatccct gagccggtac ttggtggagg aatgttagtc
    25441 atgttcggga tggtggcagt ccaaggaatc cgtatgctgt tggaagttga tttcagcaat
    25501 gataaaaatc tattgattgt tgctgtttct gttggatttg ggctgggttt caatgttacg
    25561 ccgacattat tcagtcaaat gccgcaaaca ttgcaaatgt tcactggaaa tggcatcgta
    25621 atgagtagca ttacggctat catattgaat ttgatcttta atggattgaa aaaagatgaa
    25681 gaaatattgg aagaagcctt caagaagtaa tagagcaaaa gaaaggttgt gaaatcagcg
    25741 ttttgacctg attgttagag aaacaaacgg aaaaatagtt cctcgtattt tgccgtttgt
    25801 ttgagctaat accgagggtc agctgattga acaccgttga aggaagaggt cgtgagcctg
    25861 ccgttcatct ccgagtcaca aggaacaatt tcaaaaaaaa gcttctcatg tttgttgaat
    25921 attgttcctt gtgaccgagg agttggcagg agaacaccgt ttatccataa aaaggaatgt
    25981 gacctaactt ttgtcacatt cctttttttt atctttaata aacacgaaca ataaaatgtt
    26041 tttctttgtt aacattcgaa atttctttga catcaaaaaa agtgattgtt aaaatatgaa
    26101 tgtagaaaaa ctgggaggaa aaacaatgac agcaaaaatc tctgtgatta tggggagtac
    26161 ttcagattgg gaaacaatga aagaagcatg tcaaatattg gatgaattcg gtgtgtctta
    26221 tgaaaaaaag gtcgtttctg ctcatcgaac cccagatttg atgtttgaat atgcacaaaa
    26281 cgctcgaaaa aaaggaatca aaatcattat tgcaggagca gggggagccg cgcatctgcc
    26341 gggtatggtc gcatcaaaaa caactttgcc ggttatcggt gtaccggtcc aatcacgaac
    26401 attaaatggt ttggattcgc ttctttcgat tgtccaaatg cctggaggag tacctgtagc
    26461 tactacagca atcggaaaag cgggagcaat taatgctgga ctacttgcga tacagatgct
    26521 ttccatgtat gatttagaat tggaggctaa attggctgaa agacgttcaa tgttggctaa
    26581 aactgttata gaaagtagtg atcaacttgg ttaaaccttt gttgcctggt tcaacgatcg
    26641 ggatcgtcgg tggcggtcaa ttaggtcgta tgatggcgat tagtgcaaaa gaaatgggct
    26701 ttaaagtagg tgtgctggat cctgtgagcg attgtccagc cgcacaagtt gcagattggc
    26761 atattgaaag tgtctatgat gacacctttg cgctgaaaga gcttgccaga agaacagatg
    26821 tcattaccta tgaattcgaa aacgtcagtg tagaggcgtt aaatgcgatt ttacccatgt
    26881 catttattcc tcaagggaca gacttgcttg cgattacgca agaccgcctg ttagaaaaat
    26941 cttttttgga aacgaataac atcgtgattg ctccttatgc aacgatcgtc agtccgacgg
    27001 atatccaaga tgcgattgac gggatcggct atccttgtgt tttgaaaacc actcgaggcg
    27061 gctatgatgg caaggggcag tatgttttga aaagtcgggc ggatctagct cctgccatgg
    27121 accttttaag agaaggaacc tgtgttttag aagcatggat cccgtttgaa aaagagattt
    27181 cgatcatggt ggcaggaaat ggtcaaggag atttcacgac ttttccggtc gtggaaaaca
    27241 tccaccacaa caatattctt catgaaacga ttgctccagc cgcgattgat caggatgtga
    27301 tcgaggaggc tgagcgtatt gcccgtgtga ttgcagaagc agtgagcctt tcaggtgttt
    27361 taggggtaga aatgttttta acaaaaacag gcgggctata tgtgaatgaa ttagctcctc
    27421 gtcctcacaa ttctggacat tattctattg aagcttgttc tatgagtcag ttcgatgccc
    27481 atattcgagg tatttgtggt tggccattag gagaaaaagt acgtttgata agcaatgcag
    27541 taatggtcaa tgttttggga gaacaactgc tggaaagcta tcgcttgatt gagaataagc
    27601 cagactggca attccatttc tatggaaaaa aacaagcaaa aaaaggtcgc aagatgggac
    27661 atatcactat cttgacagaa gacatccagc aaacgctgga agaaatcaaa caaacgaata
    27721 tttgggatta gaggagaaaa aacatgttag aacgttatac acgccctgaa atggggaata
    27781 tctggacgga agagaacaaa taccgtgcgt ggctcgaagt cgagatttta gcagatgaag
    27841 cttgggcaga acttggtgaa atcccgcaag aagatgtgaa aaaaattcgt gagcatgcat
    27901 catttgatat cgatcggatc ttggaaatcg aaaaattaac acgccatgat gtcgttgcgt
    27961 ttacacgagc ggtttctgaa acattgggag aagaaagaaa atgggtccat tatggtctaa
    28021 caagtacgga tgtcgtagat actgcttatg gctatctcat caagcaagca aatgatattt
    28081 tacgggaaga cttgaaacgt tttacggaaa tcattggcga aaaagcaaaa gaacacaaat
    28141 atacagtgat gatgggtcgg acacacggtg tccatgctga accaactact tttggattga
    28201 aacttgccac atggtattca gaaatgaaac ggaacatcga acgatttgaa catgcggcaa
    28261 aaggagtaga agcaggaaaa atctcaggag cagtgggaac gttcgcaaat atcccgccat
    28321 ttattgaaga gtatgtatgt aaacaattgg ggatccgtcc acaagaaatt tctactcaag
    28381 ttctccctcg ggatctacat gctgaatatt tctcagcgat ggcgcttatt gctacaagta
    28441 ttgaacgttt tgcaacagag atacgcggat tgcaaaaatc cgaaacacgt gaagtagaag
    28501 aattttttgc gaaaggtcaa aaaggttctt ctgctatgcc gcacaaaagg aatccgatcg
    28561 gttcagaaaa tatggctggt ctagccagag tggtgcgcgg acacatggtc actgcctatg
    28621 aaaatgtcac attatggcat gaaagagata tttctcattc ttcagcagaa cgaatcatct
    28681 tgcctgatac cacgatttta ttgaattaca tgttaaatcg ttttggcaat atcgttaaaa
    28741 atctaacggt ctttcctgaa aacatgaagc ggaatatgtc tgcaacattt ggtttgattt
    28801 atagccagcg cgttatgctg aaattgatcg ataagggaat gacaagagaa gaagcttatg
    28861 atcttgttca gccaaaaact gcgtacgctt gggacaacca agtgcagttc cgcccgctgt
    28921 tagaagaaga tccaaccatc acatcggtat tatctaaaga agaaatagac gatgcctttg
    28981 attatcatca tcatttgaaa aacgttgacg tgatattcaa acgagtaggt ttggaataaa
    29041 gaagagtgag ttgcaaaatt caattaaaat agaatagcaa aaataaccaa aaaagaagca
    29101 gatccgttag cggttctgct tcttttagtt tattatattt tcttatcaat aataggaacc
    29161 tgattttaaa agcggctacc tctttaccgt gcctctttac atacaaaaaa tagaggaatt
    29221 aagcgtaaat ggcttggcta ttataactaa aatgctggtt atggagatgt tgcagcaagt
    29281 tttcgagaaa agcgtattcc atctcttgtc cattgaataa gatctggata cgataatgat
    29341 ttttgaaacg cttctgttca tgaatctgac tgattgaaag ttcgcctttg tgttctgtct
    29401 gataaagaaa agtcctttta cggcattcag cacagcgctt ggttgctcgg cggatttttc
    29461 ggaatgtata aaaactagta tctttattgc ataaataaac gtctttgatc gacagcaaat
    29521 cattatatag acaggaacgt tcttcagctg ttaattctga taaaaaaagc tgaccttgtt
    29581 ttattgcatc ttccatcggc aagtgcctcc tcgaacccat tatccaaatt tttagttaat
    29641 tctaatatac catcattaag tataaatgta aacgcttgtt gatggtataa gattttcgtt
    29701 tctgataaaa cacgaacatt aaaagaatct aatgccttaa atgtacatat ttttgttgat
    29761 ttataaaaag atgattgcta aaatttaaag tgaaaaaacg aacaaaaaac gatgtatttg
    29821 cttttaatgt aaataaaaag gagagacgta catggagaag aaagaacttt tgtatgaagg
    29881 aaaagccaaa aaaatatatc ttacttctga tgagaacttg atttggatag aatatttaga
    29941 tcaagcaact gcgttgaatg gcgcgaggaa agaccagata agtggaaaag gagaattgaa
    30001 taatcaaatc acaagtgcaa ttttccgtta tctgactcaa agaggaataa aaaatcattt
    30061 tattgaacaa ttgtctaaaa ccgaacaatt agcaaaaaaa gtacaaatta tccctctgga
    30121 agtggttatc cgaaatattg cagcaggaag tttctcaaaa cgtctaggtg ttcccgaagg
    30181 taaagaattc atcaaaccaa tcattgaatt ctactataag gatgaccagt tggatgatcc
    30241 atttatcaat gaagagcata tctatttctt acaacttgca aagccggaag aaatcagtga
    30301 gctgaaagcc caagcgttgg ctattaatga aattttgacc gaattatttg ctaaggtcgg
    30361 tttggtttta gtagatttca agctagaatt tggccggtcg aatggacaaa ttttgcttgc
    30421 ggatgaagta acaccggata cttgccgatt atgggatgaa cgtacgaatg aacatatgga
    30481 caaagacgtt taccgcagag atctaggtga tttgatccca gtctatcaag aagtatataa
    30541 ccgtctatcg gaactaactg aagaaaagga gcgagtatga tgtatttcgt aaaagtatat
    30601 gtaacctata aagattcagt gttagatcct caaggggagg cagtaaaaaa agcagtccat
    30661 cgtatgggat atgaaacaat cgaagatatc cgaataggaa aatactttga gatacaactt
    30721 gctgatgcag aagaaccaat cgatacagtg attgaaacga tttgtgacaa gctgcttgca
    30781 aatgtaaata tggaaacata ccgatatgaa atcaaaaaaa tggaggaagt ataacatgaa
    30841 atttgcggtc atcgtttttc caggttcaaa ttgtgacgca gatatgttat gggcagttcg
    30901 cgagatcatg ggcgctgatg ccgaatttgt acggcatgat gagtcctctt tagaaggatt
    30961 tgatggggtt ttactgccgg gaggattctc ctatggtgac tatttgagat gcggtgcgat
    31021 tgctcggttt tctacaatca tggaagaagt gattcgtttt gctgaagaag ggaaaccagt
    31081 attcggtaca tgcaatggat tccagatatt gacagaagca ggacttttgc caggtgtact
    31141 gagacgaaat gagtctttgc attttatttg caaaactgta ccattgaaag tcaaccccaa
    31201 gacggccttt acttcagagt atgaagccga cgaaataatc tatcttccag ttgctcatgg
    31261 ggaaggaaat tatttctgtg acgaagaaac acttcaagaa ctaaaaaaaa ataatcagat
    31321 tgcctttact tataatgaca acatcaacgg cagcgtggaa aacattgcag ggattacgaa
    31381 caaagcaggg aatgtgttag gaatgatgcc tcatccagaa cgtgcagtag aagacttgct
    31441 aggatcagaa gatgggaaac ggttctttgc ttccatttta aaaaacttcg gaaaggtgac
    31501 cgtatagatg aaaacattga aagaaccgac agctgaagag atcaagaaaa aaagaatcta
    31561 tgccgaatgg ggattgactg atgaagaata tcgaatgatc gaagaagata tcttaggaag
    31621 aatgcctaat tatacggaaa ctggactgtt ttctgttatg tggagcgaac attgttccta
    31681 taaaaattct aaaccggttc tacgcaaatt tcctactagt ggacctcatg tgttgcaagg
    31741 ccctggagaa ggcgcaggca tcgtcgacat cggtgatgga ctagcagttg tcttcaaagc
    31801 agaaagtcac aatcatcctt cagccgtgga gccttatgaa ggagctgcga caggagttgg
    31861 cggaatcatc agagatattt tcagcatggg ggctcgtcca attgcgttgc tagattcatt
    31921 gaggttcggc gaattagata atccaagaac gaaatattta ctagaagaag tagttgcagg
    31981 tatcggcgga tacggcaatt gtatcggcat accgactgta ggcggcgaaa ttgcatttga
    32041 tccttgttat gaaggaaacc cattagtcaa tgcaatgtgc gtaggtttga ttgaacacaa
    32101 acatatccag aaagggcaag ccgctggcgt cggtaattcg atcatgtatg taggtgcaaa
    32161 aacaggtcgc gacggtatcc acggagcaac ttttgcctca gaagaattta atcaagaaga
    32221 agaacagcaa cgttctgctg tacaagtagg cgatcctttt atggaaaagc tgctgcttga
    32281 agcgtgtttg gaattaatct tgcatcattc ggattctcta ataggtatac aggatatggg
    32341 agcagcagga cttgtttcat ctagtgcgga aatggcttca aaagctggct caggacttat
    32401 attgacatta gatgaagtcc cacaaagaga aactgggatg acaccttatg aaatgatgtt
    32461 atcggaatca caagaacgga tgttgatttg tgttaaatca ggagaggaag aaaaaatcca
    32521 agaactattt cagaagtatg atctagatgc agtcactatc ggtaaagtaa cagacgacgg
    32581 gcaatatcgg ctatatcatc aaggagaaga agtagcaaat ctgccagtcg atgcattagc
    32641 tgaagatgca ccagtttatc ataaagaaat gaaagaacca gctcgaatcg tagaatttca
    32701 gcagttagct ccttatcagc cagttatcga agaacctgga caagtattgc ttgatctgct
    32761 gcagcaaccg acgattgctt caaaacgttc aatctacgaa acttatgatt cacaagtaca
    32821 aaccaataca gttgtccgtc caggaagcga tgctgctgtg atgagggtta gagggacgaa
    32881 taaagcgtta gctatgacca cagactgcaa cgcgcgctat ctgtatttgg atccaaaaat
    32941 cggaggacaa atagcagtgg cagaagctgc tagaaatatt attgctagcg gtggtaagcc
    33001 cttggcgatc accgactgcc tgaactatgg ttctcctgat aaaccagaag ttttttggga
    33061 attatcgact tcagctgacg gaattgctgc tgcgtgtgaa acgttaggaa ctcctgttat
    33121 ttcaggaaat gtctctttat ataatgaaac ggatggtcaa gcaatctatc caacaccaat
    33181 gattggtatg gtcggattga ttgaggatca taaacatatc actactcagg aattcaaaaa
    33241 atcaggggat ctaatctata tattaggtaa gacgtttgca gattttgatg gcagtgaatt
    33301 acaaaagatg cagcttggaa gaatcgaagg tgtgatccga aactttgatc tatccattga
    33361 aaagagaaac caagagttag tattgacagc catccaaaac ggattgattg aaagtgctca
    33421 tgactgttca gaaggtggct tggcaattgc actggcagaa tcagctttca aacatcagct
    33481 agggatatct gtacagtttg aattgtcctc tgctcaatta tttgcagaaa ctcaatcaag
    33541 atttgtgttg acagtcgcac cggaaaataa gacacgattc gaagaaatga tgggagatgc
    33601 tgctgtttta gcaggtaagg tgacggatga agcgataatc gaaatttcag caactgacgg
    33661 tcagatcaaa atagaaactg cagtagcaag aaagtgctgg gaggatgcaa tcgtatgtct
    33721 tatgaagtga agagtttaaa tgaagaatgc gggatcttcg gtgtttgggg tcatccagac
    33781 gccgctagtg tggcatactt cgggctccat agtcttcagc atcgaggaca agaaggtgca
    33841 ggtatcgtgt ccaatgatcg aggaaagctt aaggggcatc gagatttggg gctgctttct
    33901 gaggtattca aagatcaaag aaaattgcag cagttaacag gagaagctgc aatcggacat
    33961 gtacgttatg caacagcagg caatggaagt gtggacaata ttcagccttt tctattcaaa
    34021 tttcatgatc agtcgatcgg cttagcgcat aatggtaact tgatcaatgc tcgttccctt
    34081 agaaaagagt tagaggaaaa tggggcgatc ttccattcta actccgatac ggagatattg
    34141 atgcatttga tccgacgaag tgaagaaaca gattttttaa ataaattaaa aagtgctttg
    34201 aatgaagtca aaggtggctt tgcctattta ttaatgacag aaacggcaat gattgccgct
    34261 ttggacccaa atggttttcg tccattagca atcggacaaa tgaaaaacgg agcatacgtg
    34321 attgcctcgg aaacttgtgc attagaagtt attggggcac gttttataag agatgtccag
    34381 cctggtgaga tagtgatcat caatgacgaa ggaatccaga ttgattgctt tacgaaggac
    34441 acgcagcttt ctatttgttc gatggaatat atctattttg ccagaccaga ttcaaacatc
    34501 gcagggatca atgtgcatac tgcacggaaa aatatgggac ggaatttagc gaaagaatcg
    34561 ccagtaaaag cagatatggt cgttggcgta cctaattctt ctttgtcagc cgcaagcggt
    34621 tatgcggaag ctagtggaat cccttatgaa atcggattag taaaaaatca atacattgcc
    34681 cgaacattta tccagccaac gcctgagcta agagagcaag gtgttcgaat gaaattatct
    34741 gctgtacgag gagtagtgga aggtaaaaag gtgattatgg tcgatgattc aattgttcgg
    34801 ggtacgacaa gcagacggat cgtccatctt ttgaaagaag caggggcaaa agaagtccat
    34861 gtgaggattg cttctcctcc tttgaaatat ccatgttttt acgggattga tattcaaacg
    34921 cgtaaagaac tcattgcggc aaatcacacg attgatgaaa tcaaagaatg tatcggtgca
    34981 gattctttag gttttttaag tgaagagggt ctgatcgaat cgatcggtct ggaaaaagat
    35041 gctccgtatt caggattgtg tatggcttat ttcaacggag attatccgac gccgttatat
    35101 gactatgaag aaaaatatca ggaatcacta aaagaaaaag tatcgttctt ttaaaagcag
    35161 accaagaagg gagtatatga attcaatggc aaatgcatac gcaaaagctg gcgtagatgt
    35221 cgaagctgga tatgaagtag tagaacggat caagaagcat gtcaaaaaaa cggaacgttt
    35281 aggtatgatg ggcgctatag gaggattcgg cggttgtttt gatttatcgg tctttcaatt
    35341 aaaagagcca gtccttgtct cggggaccga tggcgtgggg acgaaattag cagtggcaat
    35401 cgaagcagac aaacacgata caatcgggat cgactgcgta gcgatgtgtg tcaatgatat
    35461 cttagcacaa ggcgcagagc cgttatattt cttagactac ttagcaacgg ggaaaaatga
    35521 tccgttacgt ttggaaaatg tagtaaaagg tgtagcagat ggatgtgttc aaggaaatat
    35581 ggcgctgatt ggcggagaaa cagctgaaat gcctggtatg tactcaggta atgattatga
    35641 tctagccgga tttgctgtag ggatagctga aaaatctcaa ttattgacag gagaaacgat
    35701 caacccagga gatgtgttgc ttggaatagc atctagcggg attcattcaa atgggtactc
    35761 tcttgttcgc aaaattttct ttgagcagca gcagttttct ttatcatcaa aactgccaga
    35821 attgaaaaaa acacttggcg aagaattgct tgagccaacg aaaatctatg tttcttcctt
    35881 gcttccgctg atcaaagaaa agaaaataaa aggagctgca catatcactg gtggagggtt
    35941 tgtagaaaat atcccgcgaa tgttaccaga atctatggct gcacaaatca aacttggctc
    36001 atggccgatt cttccaatat tcggctgttt acaaaaatat ggtgaaattc ctgagatgga
    36061 gatgtatgaa atcttcaata tgggcatcgg tatggtcctg gcagtcgaag aatcgaaagt
    36121 aaaagaaata gtacacgaaa ttgagcaagc tggcgaaaaa gtttatgaaa tcgggaaagt
    36181 gatcgccaaa gaaaaacatc gtgtggtgtt tataggagaa gaacgatgag aatcgctgtt
    36241 tttgcttctg gaaacggaag caactttcaa gcgctagccg attatctatc gaaaaaagga
    36301 ttggaatctt cgattgactg gttgttttgt gatcagcctg aagcatatgt tttgaaacgt
    36361 gcaactgcgt tgagtgtacc ggcagattgt ttttcaccta aagaatttga ttcaaaaaaa
    36421 gagtatgaag aagcaatttt gcataaattg aaagaaaaaa agatcgattt gatcgtatta
    36481 gctggatata tgcgaatcat tggaccggta ttgctgaaaa attatgataa aagaatcatc
    36541 aatatccatc cgtctctttt acctgccttc cctggtttgc atggcatacg tgatgccttt
    36601 gaagctggag taaaagaaac gggtgtcaca attcattata tcgaccaagg tgtagatact
    36661 ggaccaatca tccggcagga aaaagtgaga attgaacagg aggacacgtt cgattcttta
    36721 gaagaaaaaa tccatagagt agagcatcga atctatccag aagtgattag tgaaattatt
    36781 gaaaatggag gctatctgaa atgacaagag cattgatcag tgtatcagat aaaactggga
    36841 ttgtagcatt cgcccaaggt ttgattgaac aaggagtaga aatcatttca acaggaggaa
    36901 caaagcatgc gttggataaa gctgggatca aaacgctttc gatcgaagaa gtgacagggt
    36961 ttcctgaaat gatggatggc cgtgtcaaaa cacttcatcc taaaatccat ggtggtcttt
    37021 taggaagacg cgatctccct agccatatgg aggcaatgga aacggaaaat atccaaccaa
    37081 tcgactttgt ttgtgtcaat ttatatcctt tcaaagaaac gattatgaaa ccagatgtcg
    37141 aaatagcaga agctattgaa aacattgata tcggtggtcc tagtatgctc cgtagtgcag
    37201 caaaaaacca tgaatttgtc acctctgttg ttgatccatc tgactatgaa gaagtacttt
    37261 cagaattacg tcagtatggg catacttcac tttctttcag acaaaaacta gcggcaaaag
    37321 tctttcgtca tactgcggca tatgatgcac tgatcgctca atacttaacg gattgggtgg
    37381 aagaaaaaga accggaaaaa ttgacactta cgtatgaacg aaaacaaacg cttcgttacg
    37441 gtgaaaatag ccatcaagcc gctgcctttt atcagtccgc tcttctggaa aaatattcga
    37501 tcgcagcagc aaaacaactc catgggaaag aactttctta taataatatc cgagatgcag
    37561 atgctgcgct acggatcatg aaggaatatg acgagccaac ggtagtcgcc ttgaaacaca
    37621 tgaatccttg tggtattggg acagcatcta cgattttaga agcgtgggaa gcagcgtatg
    37681 aagcagatcc tgtttcaatc tttggtggga tcatcgtttt gaatcgagaa gtagatctgc
    37741 caacagcaga acagatgcat aagctgtttc tagaaatcat cattgcccct tcttatacac
    37801 aagaagcttt ggaattactg caaaaaaaga aaaacatccg attactgaca gtagactttg
    37861 cgcaggaaga aaatcaaaat cgagaagaaa cggtctcagt cttaggtgga cttcttgtac
    37921 aagatcaaga tctggcaata gaaaatgaag aagagtggaa agtcgtgaca aaacgtaagc
    37981 cgactccagc agaagcaaaa gcattagtgt ttgcttggag agctgtcaaa catgtgaaat
    38041 ctaatgcgat cgtactggca aacgaacgtc agacagtagg aatcggagcc gggcagatga
    38101 atcgagtcgg ttcagtaaaa attgcgattg aacaagcgca agagaaaatg gaaggtgcag
    38161 ttttagctag cgatgcttat tttccaatgg atgattcggt tgaatatgca gccaaacatg
    38221 gtataaaagc aatcattcag ccaggaggaa gcatcaaaga ccaagattct attgatatgg
    38281 cgaataaata cgatctagca atggtcttta ctgatgttcg gcattttaga cattagagag
    38341 gagtcggata aatgaacatt ttggtgatcg gtgcaggtgg tcgtgaacat gcaataagta
    38401 aaaaactttt agccagtccg aaggtggata cggtgtattg tgcgccagga aatccgggga
    38461 tgacaaaaga tggtatcaat tgtgtcgcta tcagtgagcg agatcattca gcgttaatcc
    38521 attttgttaa gaaagaggct attgattgga gtatcgttgg tcctgaagtt cctttgttaa
    38581 atgggatcgt cgatgatttt atggcggaag gattgaaaat cttcggtccg acaaaagcag
    38641 cagctctgat cgaaggttcg aaaagttttg ctaaacaatt aatggatact tatcaaatcc
    38701 cgacagctaa atccagaagt tttgaatgtt acgaaaaagc agcagcgtat atcaaagaaa
    38761 atggtgcgcc aatcgtagtg aaagcggacg gcttagcagc tgggaaaggt gtagtcgtgg
    38821 ctaccacagt gaaggaagcg ctggatgcgg cagaacaaat gttgaaccag catcgatttg
    38881 gggatagtag caaaaaaatc gtcgtagaag agtttttggc aggagaagaa ttctcccttt
    38941 tggcgtttgt ccgtaatacc aaggtgtatc cgatggtcat ttcgcaagat cataagcggg
    39001 catttgataa cgataaaggg ccaaatacag gcggaatggg tgcttattca ccagtaccgc
    39061 aaatttcaaa aaagatgatt gaaaaagctg tggaagaagt tttaaaacca gcggctttgg
    39121 gaatgaaaga actaggacgc tctttcacgg gtgttttata tgcgggtttg attgctacga
    39181 aggaaggacc aaaagtgatc gaattcaatg cgcgtttcgg tgatcctgaa acgcaagtcg
    39241 ttctttctcg gttgaagagt gactttgcgc aagtaatcga tgatttatta gagaatcgta
    39301 cagtagagtt aaagtggcag caagaaggat acaatgtagg cgttgtggtt gcagctgccg
    39361 gttaccccga agaatatgag acaggaatgg ttctaccaga ttttctggaa gaagagcttt
    39421 ctgtttacta tgctggagtt tcctcaaata acggcaaact agttggagca ggaggacgtc
    39481 tttatttagt agaagcatac ggtgagacga tcaaagaagc acagaaaaaa atttacaccg
    39541 cgttaatgga taaaacgaca gatggaacat tttatcgtac agatattgga gaaaaagctg
    39601 ccaatagcga actgatcgtg aagtaaatca gctgcttcca tttataaaaa atacaacaaa
    39661 cataatcgga agcgccgatt ttagtgaagc cttttcataa agagtgtggt acacttttgt
    39721 tgtgtatata aataaggagg agttcagatg aaaaaaattc aattaggtac ttctgatcag
    39781 aaagcagctg cagtgatctt aggctgtatg cgtatcaaca atgcggaaga tccagtaaaa
    39841 gtgattgaga cagcttatga aaacggcatt gatttttttg accatgcaga tatttatgga
    39901 aacggtgaat gtgaagaaat ctttgcagat gctctagcaa aaacatcaat caaacgagaa
    39961 gatcttttta ttcaaacaaa atgtggaatc gttccaggtg tgatgtttga tttttctaaa
    40021 aaacatatca ttgaatctgt agaaaaaagt ttgacccgtc tgaaaatgga ttatgtggat
    40081 gctttacttc ttcatcgtcc agatacattg gtagaaccag aggaagtagc tgaagcgttt
    40141 gatgaattgg aacggtcagg aaaagttcgt tattttggcg tcagcaatca aaaaccaatg
    40201 caaatcgaat tgctgaaaaa atatgttcgt caaccattag tagctaatca gctgcaattt
    40261 ggcttgaaac atactggaat gatcgatcaa gggattcatg tcaatatgac agatgaagca
    40321 agtatcgatc atgacggtag tgtacttgat tattcccgat tgcatgatat gacgatccaa
    40381 gcatggtcac cataccagta tggatttttt gaaggtgtat ttatcgggaa cgaaaaattc
    40441 cctgaattaa accaaacatt agaagaacta gcagctaaat atcaaacgac accaactggc
    40501 ttggcttctg cttggatttt acgccatcca gccaatatgc aagtgatcgc cggtacgatg
    40561 aacgtagggc gaatcaagga aatcgctcaa gcttcagaaa ttaatatgtc tcgggaagat
    40621 tggtacgaat tgtaccgtgc tgctggaaac attttacctt aaagattgtc ataagcaggt
    40681 caaaagggct ataaattaaa aagacagaat aacttgaaag ctgttttcag cttcgagata
    40741 ttctgtcttt ttttattgca aatggaaaga gtatcattgt acggatttcg tttattagtt
    40801 atttttgttt ttaaggaaaa gctgtgtccg atattctttt ggagaaatcc cttgtttttt
    40861 cttaaataaa taactgaaat agttagggtc attataacca atttcaaaag caatatctga
    40921 aatacgatag tctgattgct gcaataattt ttttgctaat tcgattcttt gttctgtcag
    40981 ataatcgatg aatgtttggt taaacgattg agaaaaaatc gtactgaaat gagagggact
    41041 taatgcaact tgttgagcga cccaagttaa ggacatatct ggatcggtaa aattttcttt
    41101 gataaaatcc aatgcatggt tcaaaattgg ctgatatttg ttccttgaag gctgtgaggg
    41161 cagagcagtt aagaaagaaa tgaacatata aactgtcgaa cggtaaagcg agtcgtcggc
    41221 gctgattgct aagcgttgac tggtggatgt gcaatgaacc atttcttgcg ggattttctt
    41281 ttctttcagg cagacatgct ggagtagctt agtcaagaca aataaacgat ataatcgtgt
    41341 tcgagttggt gtgccttgtg gtttcgtcaa gtgttcgatg tacttagata cttcgttttt
    41401 cgataatgaa gacaaatcaa gtgagaggtc aaagtaaaag acagaatcaa ctggaagttt
    41461 cgcttcttga atcaaatcct catagctgat aatttgttct ttaagggaac tggcggtttc
    41521 aagtatcatc tgcgtgatct caaaagatgc aggtaattcg ctgattcttt ctgcgacagg
    41581 accaatggcg acagaaaaat tatttttttg tcccttttct aattcatgga ccaacgtttg
    41641 ggctacctga taactggtct cgatcacatt ttcttcaaga gaatcaacta ttaaaaattt
    41701 gatgaatcga gaggaaacag cagagaagat cacttgttga ttctccctaa ataaaaactt
    41761 caaataatca ctgagatgag cgtaatcttc aaagtctgta ctgacttggt ttgctgctaa
    41821 taatacggtc atttttttcc ctgctaaaga gcgattgaaa gattcagcaa gtttcaatgt
    41881 ttcactgatc gtaagtgagc ctttgaacaa accgttcaag aaatgatttt ttttcaattc
    41941 gaaaacaaga tcagaagaag tgtgcgtttc tttttgctgc aaatctaaag cagttactgc
    42001 tttttctatt gcttgtttca attcgctact tttaatcggt ttaagcagat aatcatctgc
    42061 ctgtacctgg attgctcctt tcatataatc gaaatcgtcg aatccactaa taaatatgag
    42121 acgagtccaa ggaagtagtt tccttgcttc ttttgaaaaa gtcaacccgt ccatgaaagg
    42181 catttggata tcagtcagaa tgatgtcggg ctgtaagtcg ataattgaag acagtgccat
    42241 ctcaccatct ccagcttcgc cgacataagt gattggataa taagaaccaa ggacagttat
    42301 ttgttcccgc aagttattgc gaatgaggta ttcgtcttcg atgataaaaa ctttatacat
    42361 gtttgtttcc tccaattgtt ttgggagata gagggatcat cagtgtgaca gttgttcctt
    42421 ctcggtattt gctgtcaatc gtgataggtt tttgatcatt tccgtaataa agaaggagac
    42481 gcttgtttac gttgtaaagt ccgtagccga tactgaaatc tgaatcgatc cctttggcta
    42541 actctttacg tatttctgcc agtttttctg cagggatgcc aatcccgttg tctataactt
    42601 tgaattggat catctcatca attttgcatg tggtaatacg aataaatcct ttgtgacgga
    42661 cgtacttcgt tccatggtat acagcatttt caatcaatgg ctgcaagatc atttttaaaa
    42721 tagaaacagg aaaaagtgtt tctggtatat cgatcttata gtcaagcata tggccgtatc
    42781 ttatttttaa gatgactaga taatcattga tatggctaat ttctttttca agaggaatcc
    42841 agtcttgtcc tttgcttaaa gacgtacgaa aaaagccgga aagtgcatgg gtcatagtca
    42901 gagcttcttc aaagcagtct tgttcgatca aagaaataat ggcatccaaa ctgttataaa
    42961 taaaatgggg agtaatctga gcttgcaata cacgaacttc actttgtgct aaatggtatt
    43021 gtttcaaagc gttctcccgt aaagactggt ttagatcttc cgccatttta ttcatgctag
    43081 cggtaagtgt ttgtaattcc agtgtttccg gcagggttaa acgaaagcca aggttccctg
    43141 aagaaagttg ttctgacatc tcgatcagct gattgatcgg ttcttggacc tttctctcta
    43201 aaaagcgacg attctttttc gcatagaaaa aaatggtact aagaactcca agttccacta
    43261 ttgttaatag aatgagcgag cggacaagtg tcgtattttt tttgcttgct aagtcgatct
    43321 ccacacgaac aaaatcttgt aagatatctg acagcagttg agtgacagaa tctacctgat
    43381 tgatgatctc ttcattggta tccataaaat cttttgccaa gatattattg atcagctcgt
    43441 tttgataatc ttccagtgta tcgatcgtac gtagcgatac atctaacgta ctaatttctt
    43501 taagtgtatt tgtattattt ttgctctctc gaatatcatt cctaagttct tctaggatat
    43561 tttcctcatt gaactcatct acggttactt ggccagaaat caagtcccac atactttcca
    43621 aaactttttg atcaaccttc aaagcaattg catctgcttt ttggatattt gcgatcgtct
    43681 gttgataaat caaaagctgt tgcgaatata acagtatgct cagtagcaaa gggataacag
    43741 caaatagaag catggctttg tttgcttggg caaaaatctg ttttatgctt aaattttttg
    43801 tcatgatact tctccagtca ataatttctg gttgggatgt tagctatatt ctgctgggaa
    43861 aagacagtct cggaaataaa aatatcttta ggtaatgttt ttcctgtttg aatatttcca
    43921 aaataacgag taacggtatt ggctacatac cagccgacat caggtttaca ctcgactaca
    43981 caattgacga ttcctgagcg tagcttctcg atcatatctt cttgtccatc aatagttaca
    44041 atgatcaaat cttctccagg tttgattgat gtttctttta tcgcagctaa agcaccgtga
    44101 gtcatttcat cactatggct gtataataca tcgatttcgg ataaatcatt tgtttctaaa
    44161 tatcgtttca tctggtcttt tcctttcatc cgaataaaat caccgacaat tcggtgaaca
    44221 atagtcatcg gaggtttatg gtcgatggtt ccatcttttt ttagggttgg tttacgattg
    44281 attgattcca aaaatccttc ggagcgtagc ttggtcggtg tggagttctc tgaaccagca
    44341 agttctagga ttctgatttc ctgtttcttt gaatttgtga agtgattgct gacaaataaa
    44401 cccgcccgat ttccttctgc tttgaaactg gggccaatgt gggtgacgaa aagctcggga
    44461 tcattcgttt gaatatgtct gtcgacaagt atgacaggga tccctgctcg ctttgcttct
    44521 tttaatacag agtcccaccc ggattcttgc aaaggagtaa aaatgatcat atccacttta
    44581 taggcaatga acgtccgtat atcttggatt tgccgttcct gattcatgta gccatttcgg
    44641 tagagaactt ggtaatcttc tttttcaagc gcagtgataa ttgattcagt atgctttttc
    44701 cgccagctac tctccgttcc tgattgcgaa aaaccaacga ctagtgggtc aggtgcttcc
    44761 tttgtattag gtgtacaccc catcatttgg taagcgatta caacaagggc taaaaaaaat
    44821 aaaacacgtt tcactttaaa tcactccatc tggatttgtt agtactatct tatcagataa
    44881 aagggaaaat caaagtcaaa aggaatcaat cgaaaaaaat ccaagaaact taaaaaaatc
    44941 caagacaaaa aaagtacacc taaaaaattt aaagtaaatc ccataaaaaa atcaatgtta
    45001 acgctttctt tattagctat actcggtgta tcaattgaaa tgggggataa aaaatgaaaa
    45061 acaaatttag aaagattgtt gggactatcg ctattttgac cgcaagcatt ctattaagtg
    45121 cgtgtggcaa ttcaggatct gcagatgata gtacggggta tgtaggaatc gctatgccga
    45181 ccaaatcagc agaaagatgg atcgctgatg ggaacaacat ggtctcggaa ttagaaaaac
    45241 tgggttataa aacagatctt caatatggag aagataaagt agaaaatcaa gtagcacaaa
    45301 tagaaaatat gattacaaaa ggggtagata cacttgtgat tgcttctatc gatggttcgg
    45361 ctttaactga tgtgttagct aaagcaaaag aagcagatat caaggtgatc gcctatgatc
    45421 gtttactgat gaattcagaa aatgtcgatt attacgcgac ttttgataat tttggagtag
    45481 gtgtatcgca agcagcttat attgaagagc acttaggatt gaaagaagga aaaggaccat
    45541 tcacgattga attatttggt ggctcgcctg acgacaacaa cgcactaatc aattacaacg
    45601 gagtgatgtc tgttttacaa ccatatatgg acaacggtca gttggtcgtg ccatctggac
    45661 agacgagttt cagtcaaatc gctacgcttc gctgggatgg ttctacggcg caagcaagga
    45721 tggataatct gttaagcgca aattatactg accaaacatt ggatgctgtt ttgtcgccat
    45781 atgatccaat aagtttagga atcatttcgt cattaaaagg cgtggggtat gggagtgaaa
    45841 gtaaaccttt acctgtgatt actggacaag atgcgacagt tgctggcgtt aaatcgatca
    45901 ttgccgggga acaaacacaa acgatcttta aagacacacg tatcttagca aaaaatacga
    45961 ttgaaatgat caaagcaatc agtgatggag aagaggttcc ggtcaatgat actgaaactt
    46021 atgataatgg ggtgaaaact gtccctacgt atttagcaaa tacagtatct gtcgataaag
    46081 ataattatca agcagagctg atcgacaccg attattataa agaatccgat ctgaaaaatt
    46141 aagaggaacc aaacaaggaa ctaaagagat ctaagtcaga gagaggataa gaattatggc
    46201 ggattgtatt ttaaaaatgg cagatatcac caaaaaattt tctggaatcc gagcgttaga
    46261 ttgcgtgaat ctgatgattg aacgtgggga aatccatgca ttgtgcggag aaaacggagc
    46321 gggaaaatca acattgatga atgtcttgag cggcttgtat ccatatggct cctatgacgg
    46381 ggaaatcttt tatgatggaa agctgtgtca atttaaagat ttgaaagata gtgaagaaaa
    46441 aggcatcgtc atcattcatc aagaattagc actaagtcct tatctttcaa taaaagagaa
    46501 tatttttctc ggaaatgaac aagcagaaag aggcgtgatt gattgggaac tgacagaaaa
    46561 gaaaacatat gaactgctga ataaagtcgg tttgtctatc gacccgaatc ttctagtatc
    46621 ccagattggc gtagggcaac agcaactagt tgaaatagct aaagcattat caaaatccgt
    46681 ccgtctgttg attttggatg agcctaccgc tgcattgaat gaagaagaaa gcgcgaattt
    46741 gctgagattg atccgagagt tgaaagagca aggtgtaacc tccatcatta tttcacataa
    46801 gctgaatgaa atcgtggata tcgcagatcg aatcaccatt ttacgggatg gacagacgat
    46861 tgagacacta gaaaatgaaa tgatcagcga agagcatatt attcgcggaa tggtgggcag
    46921 agatttagtt gatcgttatc ctgaaagaaa accggatatt ggagaagttt attttaaagt
    46981 agaagactgg acggtctatc accctcttga tcacgagcga atcataaacg accacatcaa
    47041 cttgcatttg aaaaaaggag agattgtagg aatagctgga ctgatgggag cgggcagaac
    47101 tgaattcgct atgagtctat ttggacattc ttatggaagc aagatatccg gacgtatatt
    47161 aaaggaaggg gcagagatat cagttaagac cgttccttca gccattgcaa acggactcgc
    47221 ttatgtctcg gaggaccgaa aatctttagg actgaatctg ctgatggata ttcgggaaaa
    47281 cgtgacgctg agcagtttgg ataaaataag tcaaaaagga gtattagata aagaaaaaga
    47341 agtaactgag gcagaaaatt tccggaaaaa gatgcgtata aaaacaaact ccatccatca
    47401 aattgtcagt agtcttagtg gtgggaatca acaaaaagtc gttttatcca agtggctgat
    47461 gacacaaccc gatattcttt ttttggatga gccaacccgt gggatagatg ttggggcaaa
    47521 atatgagatc tacacgatta tcgaagaaat ggcagcaaat ggcaaagctg tctgtcttat
    47581 ctcttctgaa cttcctgaaa tattagggat gtgtgatcga atctatacga tgaacgaagg
    47641 acgtattaca ggagaagtct cgcgagaaga tgcagatcag gaagtcttga tgaaattaat
    47701 gacgaaagaa gaggtgggtt aagttggaac aggcaaaaaa tcttacggat aaaaaagaga
    47761 aaaaactaaa tttgaaaagt agagcagctg atctttttgg tcgctacagc atggtgatca
    47821 ttttagcggg actacttatt gcgtttcaat tgatgacgga tggtattttc tggcgtccat
    47881 tgaatataac taaccttgtt ctgcaaaata gccatatatt agtactagct gcagggatgt
    47941 tattagttgt gctcttaggt catgtggatt tgtcggtagg gtcagtgatg gcgtttgttg
    48001 gtgcaattgc gggcatgatg atggtcaatc gccaaatgaa cccgtggatg acagttatcg
    48061 catgtcttgc agtaggcggg ctgatcggcg catggcaagg tttttgggta gcttatgcag
    48121 ggataccggc gtttatcgtg actttagcag gattgctgat gtttcgtgga ctgacacaag
    48181 ttgttctagg aggccaatct ctagcacctt ttccagctgt tttccaaaaa atgtctacgg
    48241 gttatttacc agacttaagc gaaggaagca gccttcactt cttgacattg attttaggaa
    48301 tgatcatcac gatcgtgtta gttgttggtc aatggcgaac aagaaatcgt cggaaaaatc
    48361 atttatttga agtggaatct ccaacagctt tcttggtaaa ggcggtattt acagcagtcg
    48421 ttgtactagg aataacttat atctttgcct cttatcaagg gtatccgatt attttgatca
    48481 ttcttggttt gatcgttgca gcatatgggt ttttgacaaa taaaacagta gcaggtcgac
    48541 agatttatgc gacgggcgga aatcgaaaag cggctgaact atcagggatc aaaacaaagc
    48601 gaatcacgtt ctgggtattc gtcaacatgg gagtcatggc agctttagct ggattagttt
    48661 tagctgctcg attgaatgcg gcaacacctc aagcaggtac atcgatggaa ctggatgcaa
    48721 tggcggcagt atattttggt ggtgcttcga cttccggcgg cattggaaca atcatgggtg
    48781 cgattgtcgg tggcttggtc atgggtgtat tgaacaatgg catgtcgatt ttaggcgtcg
    48841 gggtagactg gcagcaggca atcaaaggat tgatcttgct tttagccgtt gtgctagact
    48901 tgtataataa gaaaaaacga acaacgtagg agaacgccgg ttattcagaa aaaagactgt
    48961 gacaagacat ttgtcacagt ctttttttcg ctaaaagcga acattcattg tttcgtttgt
    49021 atagactaaa aataatatat gtttttttac cattcgtata aattctcttt ttgctcttga
    49081 tttttttgct taattgagtt aaattacata gggcgctaaa aaaagccttg attaaattaa
    49141 taatgttcgt gttttgctgg atgtgaacat caggaggata atgtaatgaa agtatttgat
    49201 tacgaagata ttcaattaat tcctaataaa tgtatcgtaa atagccgttc tgaatgtgat
    49261 acaacggtca cattaggtaa acatacattc aaaatgccag tagtgcctgc caacatgcag
    49321 acgattattg atgaaacaat cgcagaattc ttagcggaaa atggttattt ctatatcatg
    49381 catcggtttg atgaagcagc tcgaattccg tttatcaaaa aaatgaaaaa gcgtggattg
    49441 attacctcga tcagtgttgg cgtcaagaaa gaggaatatt cctttataga gaagttggca
    49501 gaagaatcgc taaatcctga ttatatcact atcgacatcg cgcatggaca tgctaactca
    49561 gttattgata tgattcagca tattaaaaaa tatttgcctg aaacttttgt gattgccggt
    49621 aatgttggta caccggaagc cgtccgtgaa ttggaaaatg cgggcgcaga tgcaacaaaa
    49681 gtcgggattg gtccaggaaa agtatgtatt acaaaaatca agacaggctt tgggactgga
    49741 ggctggcaac tggcagctct tagatggtgt gcgaaagcag ctcgcaagcc gatcatcgct
    49801 gacggtggga tcagaacaca tggcgatatt gcaaaatctg tccgttttgg tgcgacaatg
    49861 gtcatgattg gttcattgtt tgccggtcac gaggaatctc ctggcgaaac aaaagtcgaa
    49921 aatggaatcg tgtataaaga atactttggt tctgcttcag aattccaaaa aggcgaaaaa
    49981 cgaaatgttg aaggaaagaa aatctggatc caacacaaag gatctttgaa agatacacta
    50041 gtggaaatgc aacaagacct tcaatcatct atttcttatg ctggaggaag agatctggaa
    50101 gccattcgaa aagttgacta tgtcatcgtg aaaaattcga tttttaatgg agacacaatc
    50161 taaaaaataa ataaaatatt aggatgttgt gaaaaagttg tcctgcatta agtaataaga
    50221 acagccaaac aaaaatagtt ctttatatgt tttgtttggc tggaattatt atcgcagata
    50281 caagcttttg aacatcatta ttcagaataa gggactgtga taagattttt gtcacagtcc
    50341 cttatgtttt gcaaaaaaag tttttctttt ttctcgtaaa ttcggtaaaa tcaaaagaaa
    50401 aaatagacaa tagaaaagag aagcttatgt gggaaaaagt agagaaaaaa caaggaccga
    50461 tttatcgtca aattatggaa cagattatga gagaaatcga aaacggaagg atcaatcctg
    50521 gaagtcaact tgaatcagaa cgaaaactag ggcaactttt tggtgtgaat cgttccaccg
    50581 ttgtccatgc attggaagag cttagggagc ttggggtact aacgagcaaa cgtggcagcg
    50641 gatggtttgt taatcagact gaatggggga aattcgctgt tccaagaatc gattggcggc
    50701 aaatgatctc tcctcgttat gagcagacgg actggtatga gcaaaaaata agagaagcaa
    50761 agcagatcca aaaaacatcg tttcttgatt tatacgctag cgagatgcca gatgagcttt
    50821 tgccgaattt tgagtttcct tcttattctc ttgaagaaat cttagcggaa gaaaaagaaa
    50881 tggatctttt aggctatact ccgttacgta acaaaatcaa acggtatttg gaaagtaaat
    50941 ttgaattggc attttccccc aaccagcttc ttgtgactgg tggcggtcag caagcaatct
    51001 ttttgatttt acaaacgatt ctttcagctg gagaggggat agctgtagag tcaccttctt
    51061 ttttttaccg attacctctt ttcaaagctt caggaacgcg tttatttggg ataccgatgg
    51121 atgaagaagg gatcgactta gaaaaactga aagaatcgat ccataaaaat aaattgaaag
    51181 cagtgcttat caacccgaat tttcaaaacc ctacaggaaa agtgatgagt caaaaacgac
    51241 gagaaaagct agtagaattg tgtcgacagt atcggcttcc aattattgaa gacgatgtat
    51301 ttacggatct ttcatttcta aaccaagaag aagtccttcc tatacgaacg attgatccgg
    51361 ataatgtgtt atacgtcggt tcgttatcta gggttttagg aaaaacaacg aaaatcggtt
    51421 gggtgatagg acctgagact tttatctcgc aactagctca agcgcaagaa atgatggagt
    51481 tttcgatgaa tatccttaca caaatcacag tggctaatgt atttaatgaa gcaattgatt
    51541 tgaaaatgca agatgtaaga aaaaagttga aagaaaaaag ccgactgata agcgcttggg
    51601 gagattcaca atcgtttttc aagcttcatt caattttagg tggttactat gcctggatta
    51661 cttgggatgg gaaaaaacta gaccccgaac tagcagaaaa agtagtagat atgggtcttg
    51721 gaattgcacc aagtctactt tttgggcaag atataaatgg aatcagaatc aattacagta
    51781 ggctggatga aaaccaaata ccgttgtttt attcaaagat gaaaattcta gaaaagtggt
    51841 taacagaata atttcacaaa gtaaatagat taaagaatat aacagcttgg atttaaattt
    51901 caagctgttt cttttttttc ggaaaaagaa tacgatataa agaaactaag aaaaaaccat
    51961 tatttgtacg tccgttcacg atgtgtaaat aaaataaatg tttgcaacaa aactgtataa
    52021 caccaacgtt tttaaataga aacagatggt tgtaagtttg attagaaaat agaaaaaatg
    52081 cagatgaaat ataaaacgct ttccttattg tgattttatt cactaatgct gttaatatgc
    52141 ggattggcat aatttccttt catcgtgaaa atttgtgctt ttagcttgta tttctcataa
    52201 atccatgata atttattaat gaagacggtt aaatagtgat acagttttat tcactgtttc
    52261 aatcgcagaa tgaagagatt ttcttataga tgagagagga atgtgtgaca aatggcaaac
    52321 aaaaaagcac cgatcgactt ccaagcactg ctagatgaag tcaatgcaga tttcccagta
    52381 tatcaagcac ttgatcaaga tggaaacgta gttaacaaag atttagtacc tgatttatcc
    52441 gatgatgaac tcgttgaatt aatgactcat atggtatggt ctcgtgtatt agaccaacgt
    52501 tcaacagcgc tgaaccgcca aggacgccta ggcttttttg caccaactgc tggacaagag
    52561 gcaagtcagc tggctagtgc ttacgcattt gataaagaag atgttcttct tccaggttat
    52621 cgggatgtac ctcaactagt aaaacatggt ttacctttat ctcaagcttt tttatggtct
    52681 cgaggacatg ctgcaggtaa ttcttatccg gaagaattga aagctctacc accacaaatt
    52741 atcatcggtg cacaatatgt acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac
    52801 aagaaaaatg tcgtcttcac ttatactggt gatggcggtt catcacaagg tgatttttac
    52861 gaagcaatca atttcgctgg cgcttaccat gcaaatgccg tattttatat ccaaaacaac
    52921 gggtatgcga tctctacacc tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa
    52981 gctgtcgcag ctgggattcc tggtattcaa gtcgatggaa tggatccgct tgcagtatat
    53041 actgtatcta aaatggcacg ggaatgggcg atcagtggaa atggaccagt cctaatcgaa
    53101 acactgactt atcgttatgg accacataca ctatctggtg acgatccaac ccgctaccgt
    53161 acaaaagaca cagatgacga ttggcagaaa aaagatccat tgatccgctt ccgtaaatat
    53221 ttgacggaaa aaggactttg gtctgaagaa aaagaagaac aagtcatcga agcaactaaa
    53281 gaagaaatca aagcagctat tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat
    53341 ttcttgaaaa acatgttcga agtatctcca caatcaatca aagaacaaat tgcaatttat
    53401 gaagcgaagg agtcgaaata atcatggcac aaaaaacaat gatccaagca atcacagatg
    53461 ccttagcact tgaactagaa aatgacgaga atgtcgttgt ttttggtgaa gatgttggga
    53521 aaaacggagg agttttccgg gcaactgaag gattgcagga aaagttcgga gaagaccgag
    53581 tattcgatac tcctttagct gaatctggaa tcgcaggatt gagtttcggt cttgcgttag
    53641 aaggatttcg tccagttcct gaaatccaat tctttggttt tattttcgaa gcaatggacg
    53701 aagtagtagc tcaaatggct cgtacaagat atcgaatgag cggcactaga aatctaccca
    53761 tcacaattcg ttcgccattt ggtggcggtg ttcatacacc agaattgcat tcggataatt
    53821 tagaaggttt gatcgctcag tctcctggta ttcgagtagt gattccttcc aacccttatg
    53881 atgcaaaagg actattgatt tcttctatta gaagtaacga tccggttgtt ttcctagaac
    53941 acatgaaact atatcgctca ttccgagaag aagtgccgga tgaagcttac gaagttcctt
    54001 tggataaagc agcagttact cgtgagggaa cagatgtatc tattatcaca tacggagcaa
    54061 tggtgcgtga agcaatcaaa gcagcagaca atcttgcaaa agaaaatata tctgttgaaa
    54121 tcattgatct tcgtacagtt gcgccattag atgtagaaac gatcattcaa tccgtggaaa
    54181 aaactggtcg cgtagttgtt gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag
    54241 tagtttctga gatctcagaa cgcgctatcc tttcattaga agccccaatc ggacgagttt
    54301 ctgcacctga tacagtcttc ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa
    54361 aagatatcga agacaaagta aaagaaatcg cggaatttta agaaaagaag ggacgaaaca
    54421 aagtcatggc ttatcaattt aaattgccgg atatcggtga aggaatcgca gaaggtgaaa
    54481 tcgtcaaatg gttcgtcaaa ccaggagata cgatcaatga agatgataca ttactagaag
    54541 ttcaaaatga caaatcagta gaagaaatcc cgtcacctgt tacaggaacg gttaaaaatg
    54601 tgattgttcc agaaggtacc gtagcaaatg ttggggatgt attagtagaa atcgatgcac
    54661 caggacacga agacaatgaa ggggacagtg gtgtagccgc agaatcccaa actccagcta
    54721 aaccggctgc tgaacccact gttgatacag aatcagctgg atcgtcaagc gaaggagtct
    54781 tccaatttaa gtt
//
LOCUS       DACEDD010000014        52140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEDD010000014 DACEDD010000000
VERSION     DACEDD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.014"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IWO45_000959"
     CDS             330..1175
                     /locus_tag="IWO45_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0332447.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IWO45_000960"
     CDS             1456..2676
                     /locus_tag="IWO45_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0332448.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IWO45_000961"
     CDS             2673..5393
                     /locus_tag="IWO45_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0332449.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IWO45_000962"
     CDS             5377..6321
                     /locus_tag="IWO45_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0332450.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IWO45_000963"
     CDS             complement(6390..7400)
                     /locus_tag="IWO45_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0332451.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IWO45_000964"
     CDS             complement(7580..7924)
                     /locus_tag="IWO45_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0332452.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IWO45_000965"
     CDS             complement(7917..8351)
                     /locus_tag="IWO45_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0332453.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IWO45_000966"
     CDS             8489..9226
                     /locus_tag="IWO45_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332454.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IWO45_000967"
     CDS             9219..10430
                     /locus_tag="IWO45_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332455.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IWO45_000968"
     CDS             10637..11416
                     /locus_tag="IWO45_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0332456.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IWO45_000969"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IWO45_000969"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0332457.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO45_000970"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO45_000970"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0332458.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO45_000971"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO45_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0332459.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IWO45_000972"
     CDS             15018..16067
                     /locus_tag="IWO45_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0332460.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IWO45_000973"
     CDS             16064..16342
                     /locus_tag="IWO45_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0332461.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IWO45_000974"
     CDS             16317..16787
                     /locus_tag="IWO45_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0332462.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IWO45_000975"
     CDS             16804..17106
                     /locus_tag="IWO45_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332463.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IWO45_000976"
     CDS             17123..17494
                     /locus_tag="IWO45_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0332464.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IWO45_000977"
     CDS             17466..17825
                     /locus_tag="IWO45_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332465.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IWO45_000978"
     CDS             17997..19004
                     /locus_tag="IWO45_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0332466.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IWO45_000979"
     CDS             19029..20213
                     /locus_tag="IWO45_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0332467.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IWO45_000980"
     CDS             complement(20650..22233)
                     /locus_tag="IWO45_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0332468.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IWO45_000981"
     CDS             complement(22811..22954)
                     /locus_tag="IWO45_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0332469.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="IWO45_000982"
     CDS             complement(23176..23655)
                     /locus_tag="IWO45_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0332470.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="IWO45_000983"
     CDS             complement(23728..24012)
                     /locus_tag="IWO45_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332471.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="IWO45_000984"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO45_000984"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="IWO45_000985"
     CDS             complement(24356..25642)
                     /locus_tag="IWO45_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0332472.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="IWO45_000986"
     CDS             25780..26451
                     /locus_tag="IWO45_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0332473.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="IWO45_000987"
     CDS             26417..26905
                     /locus_tag="IWO45_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0332474.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="IWO45_000988"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="IWO45_000988"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0332475.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="IWO45_000989"
     CDS             27866..28630
                     /locus_tag="IWO45_000989"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0332476.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="IWO45_000990"
     CDS             28863..31076
                     /locus_tag="IWO45_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0332477.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="IWO45_000991"
     CDS             31087..31533
                     /locus_tag="IWO45_000991"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0332478.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="IWO45_000992"
     CDS             31704..32963
                     /locus_tag="IWO45_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0332479.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="IWO45_000993"
     CDS             complement(33052..33999)
                     /locus_tag="IWO45_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0332480.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="IWO45_000994"
     CDS             34393..35685
                     /locus_tag="IWO45_000994"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0332481.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="IWO45_000995"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="IWO45_000995"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0332482.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="IWO45_000996"
     CDS             37578..38453
                     /locus_tag="IWO45_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0332483.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="IWO45_000997"
     CDS             38719..40602
                     /locus_tag="IWO45_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0332484.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="IWO45_000998"
     CDS             complement(40677..40961)
                     /locus_tag="IWO45_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0332485.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="IWO45_000999"
     CDS             complement(40951..41685)
                     /locus_tag="IWO45_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0332486.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="IWO45_001000"
     CDS             41814..42437
                     /locus_tag="IWO45_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0332487.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="IWO45_001001"
     CDS             42629..43750
                     /locus_tag="IWO45_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0332488.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="IWO45_001002"
     CDS             43757..46885
                     /locus_tag="IWO45_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0332489.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="IWO45_001003"
     CDS             46904..50098
                     /locus_tag="IWO45_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0332490.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="IWO45_001004"
     CDS             complement(50244..50951)
                     /locus_tag="IWO45_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0332491.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51716..52114
                     /gene="spxA"
                     /locus_tag="IWO45_001005"
     CDS             51716..52114
                     /gene="spxA"
                     /locus_tag="IWO45_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0332492.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17112 a         9068 c        10788 g        15172 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt
    51421 aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt
    51481 catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag
    51541 cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt
    51601 atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta
    51661 ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt
    51721 aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga
    51781 acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt
    51841 aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa
    51901 agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact
    51961 tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca
    52021 agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga
    52081 attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
//
LOCUS       DACEDD010000015        49682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEDD010000015 DACEDD010000000
VERSION     DACEDD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.015"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            257..532
                     /locus_tag="IWO45_001006"
     CDS             257..532
                     /locus_tag="IWO45_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332493.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="IWO45_001007"
     CDS             545..2623
                     /locus_tag="IWO45_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0332494.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="IWO45_001008"
     CDS             2783..4669
                     /locus_tag="IWO45_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0332495.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="IWO45_001009"
     CDS             4681..5628
                     /locus_tag="IWO45_001009"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0332496.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="IWO45_001010"
     CDS             5648..6175
                     /locus_tag="IWO45_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0332497.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="IWO45_001011"
     CDS             complement(6238..6891)
                     /locus_tag="IWO45_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0332498.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="IWO45_001012"
     CDS             7023..7865
                     /locus_tag="IWO45_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0332499.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="IWO45_001013"
     CDS             8023..8919
                     /locus_tag="IWO45_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0332500.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="IWO45_001014"
     CDS             8922..9635
                     /locus_tag="IWO45_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0332501.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="IWO45_001015"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="IWO45_001015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0332502.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="IWO45_001016"
     CDS             10170..10397
                     /locus_tag="IWO45_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0332503.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="IWO45_001017"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="IWO45_001017"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0332504.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11725)
                     /locus_tag="IWO45_001018"
     CDS             complement(11183..11725)
                     /locus_tag="IWO45_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0332505.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="IWO45_001019"
     CDS             complement(11962..12360)
                     /locus_tag="IWO45_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0332506.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="IWO45_001020"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="IWO45_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0332507.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELEVLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="IWO45_001021"
     CDS             13473..14240
                     /locus_tag="IWO45_001021"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0332508.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="IWO45_001022"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="IWO45_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0332509.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="IWO45_001023"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="IWO45_001023"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0332510.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="IWO45_001024"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="IWO45_001024"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0332511.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="IWO45_001025"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="IWO45_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0332512.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="IWO45_001026"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="IWO45_001026"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0332513.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="IWO45_001027"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="IWO45_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0332514.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="IWO45_001028"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="IWO45_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0332515.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="IWO45_001029"
     CDS             22691..23566
                     /locus_tag="IWO45_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0332516.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23698..24336)
                     /gene="plsY"
                     /locus_tag="IWO45_001030"
     CDS             complement(23698..24336)
                     /gene="plsY"
                     /locus_tag="IWO45_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0332517.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24463..24897
                     /locus_tag="IWO45_001031"
     CDS             24463..24897
                     /locus_tag="IWO45_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0332518.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25063..27108
                     /gene="parE"
                     /locus_tag="IWO45_001032"
     CDS             25063..27108
                     /gene="parE"
                     /locus_tag="IWO45_001032"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0332519.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27120..29570
                     /gene="parC"
                     /locus_tag="IWO45_001033"
     CDS             27120..29570
                     /gene="parC"
                     /locus_tag="IWO45_001033"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0332520.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29847..32078
                     /gene="pflB"
                     /locus_tag="IWO45_001034"
     CDS             29847..32078
                     /gene="pflB"
                     /locus_tag="IWO45_001034"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0332521.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32322..33083
                     /gene="pflA"
                     /locus_tag="IWO45_001035"
     CDS             32322..33083
                     /gene="pflA"
                     /locus_tag="IWO45_001035"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0332522.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33168..34094
                     /locus_tag="IWO45_001036"
     CDS             33168..34094
                     /locus_tag="IWO45_001036"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0332523.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34276..35169
                     /locus_tag="IWO45_001037"
     CDS             34276..35169
                     /locus_tag="IWO45_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0332524.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35303..35428
                     /locus_tag="IWO45_001038"
     CDS             35303..35428
                     /locus_tag="IWO45_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0332525.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35562..36194)
                     /locus_tag="IWO45_001039"
     CDS             complement(35562..36194)
                     /locus_tag="IWO45_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332526.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(36342..36884)
                     /locus_tag="IWO45_001040"
     CDS             complement(36342..36884)
                     /locus_tag="IWO45_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0332527.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            37019..37408
                     /locus_tag="IWO45_001041"
     CDS             37019..37408
                     /locus_tag="IWO45_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332528.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            37412..38176
                     /locus_tag="IWO45_001042"
     CDS             37412..38176
                     /locus_tag="IWO45_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0332529.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            38305..39252
                     /locus_tag="IWO45_001043"
     CDS             38305..39252
                     /locus_tag="IWO45_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0332530.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            39382..40122
                     /locus_tag="IWO45_001044"
     CDS             39382..40122
                     /locus_tag="IWO45_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332531.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(40413..41081)
                     /locus_tag="IWO45_001045"
     CDS             complement(40413..41081)
                     /locus_tag="IWO45_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0332532.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            41215..41502
                     /locus_tag="IWO45_001046"
     CDS             41215..41502
                     /locus_tag="IWO45_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332533.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            41918..43456
                     /locus_tag="IWO45_001047"
     CDS             41918..43456
                     /locus_tag="IWO45_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0332534.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            43453..44418
                     /locus_tag="IWO45_001048"
     CDS             43453..44418
                     /locus_tag="IWO45_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332535.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            44405..45727
                     /locus_tag="IWO45_001049"
     CDS             44405..45727
                     /locus_tag="IWO45_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0332536.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      45790..45894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            45968..46711
                     /locus_tag="IWO45_001050"
     CDS             45968..46711
                     /locus_tag="IWO45_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332537.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            46726..47718
                     /locus_tag="IWO45_001051"
     CDS             46726..47718
                     /locus_tag="IWO45_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0332538.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            47730..48467
                     /locus_tag="IWO45_001052"
     CDS             47730..48467
                     /locus_tag="IWO45_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332539.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            48457..49179
                     /locus_tag="IWO45_001053"
     CDS             48457..49179
                     /locus_tag="IWO45_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0332540.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(49263..>49682)
                     /locus_tag="IWO45_001054"
     CDS             complement(49263..>49682)
                     /locus_tag="IWO45_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0332541.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        17145 a         8507 c        10411 g        13619 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagactatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaagtgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgatttg tcacactccc gttttatctc tttggaatga aaaagtatca
    23701 ggattttttc ttgctgctat tcaaaccaaa cgggattcga ctttcagtat ggtttctgat
    23761 acgcttgata ttgtctttat gccgataaaa gataaaggaa gaaactaaaa aggcaatgat
    23821 cgtcagtaac caattgggct tagccaaaat cacaggagct acataaggaa ggacgatcgt
    23881 agataaagtg atcaatactg cactgatcat actgctcaaa ctcaccatac ttgtacaaaa
    23941 taaactaacg ataaagatga tacaagaata aacaaaaaat acaggattaa atcctaaaag
    24001 cattcctgca cttgtcgcaa cagcttttcc tcctttgaac ccagcaaaaa tcggaaatgt
    24061 atggccaaga atggcgcata ccccaaacca aagagggtta atcccagaaa ggtggaagat
    24121 catcggcaaa caagtagcca atgtaccttt taaaatatcc ataagcagga caacaatccc
    24181 ggctgttttg cctaatacac gaaatgtatt ggtcgttcca gtattaccgc tcccgtgttg
    24241 tcggatatct tttttgaaaa acaatttgcc aatccagaca ccggaaggaa tagaacctaa
    24301 aagatatgct aaaataagta ataagatcaa tttcattttt taccctcttt caagttgaaa
    24361 actatccttt attttagcat gaataggtaa caggaacaat tgaaaaaatg aaagatacaa
    24421 gtataataga tggaataaaa agaaaagcaa ggggtggcag aaatgtctgt aaaaaatcca
    24481 acacaagaaa cgattttcaa ctatttggaa gaagcaaaaa atattgccgt cgtaggcttg
    24541 agcaataaac cagaacgaac aagttacatg gttgcacagg tattgcaaga caatggatat
    24601 aaaatcatac cagtaaatcc attattggct ggtgaagaaa ttctaggcga gaaagtctat
    24661 gcgaatttga cagaaatcga tgatccaatc gatatcgtag atgtatttag acggagtgaa
    24721 tttttacctg aaatagcagg tgattttttg aaaactgatg ctaaagtgtt ctgggcacaa
    24781 ttagggattg aaaacaaaga agcagctgag atgctaagaa aagcaggacg tacagatatc
    24841 gtaatggatc gttgtattaa gattgaactt ggtaagttga aagaaaacca aaaataaaaa
    24901 tgaccacaaa gaagagatga tcgatagagg ggaatgtcga aaaagcggtt aggagataat
    24961 taaaattgat ttctcgtcta ccctcttttt tttcacgacc ttttctagta tgataatcgt
    25021 ggtgcggttt tgccgcaaag tattattgaa ggagcgtttg ccttggcaaa aaaagtaaac
    25081 aacgaataca atgactcgtc catccaagta ttggaaggat tagaggcagt tagaaaaaga
    25141 ccagggatgt acattggatc cactgacagc cgaggcttgc atcatttagt ctatgaaatc
    25201 gtcgataatg cagttgacga agcattatca ggttatggca gtgagattga tgtgaccatc
    25261 catgaagaca atagtataac tgttgcagat agcggacgtg ggatgcctgt aggaatgcat
    25321 gcttccggaa taccaacagt tgaagtgatt tttactgtgc tgcatgctgg cggaaagttt
    25381 ggtcaaggag ggtacaaaac ttctggtgga ctgcacggtg taggagccag tgtggtaaat
    25441 gccttatcga aatggctaat tgtaacgatc gttagagatg gcgtagagta tcagcagaaa
    25501 tttaaaaacg gcggtaaacc tgacggtact ttgaaaaaga taggaaaaac aaaaaaagca
    25561 aacgggacga ctgtccattt tttaccagat gatacaatat tcagtacaac gaaattttca
    25621 tatgaaattt tagccgaacg gttaagagaa tctgcctttc ttctaaaagg cgttaaaatc
    25681 tctctttctg atcttcgtgg agaagaacca gtcaaggaaa tttttcatta cgaagaagga
    25741 atcaaagaat ttgttgatta tctgaatgaa gaaaaagata cattgacacc agttgtttat
    25801 ttttcaggag aaaaagaagg tatcgaagta gaggtcgctt atcaatacaa tgacggttat
    25861 tcagaaaatg ttttatcttt tgtcaataac gttcggacaa aagatggggg aacccatgaa
    25921 gctggtatga aagcggcgat gacgaaatct tataatgaat atgcgagaaa agttggtttg
    25981 ctgaaagaac gggacaagaa tctggaaggt agtgatttca gagaagggtt agctgctgta
    26041 ctttctatcc gtgtacctga aaatcttctg caatttgaag ggcaaacaaa agaaaaactt
    26101 ggtacgccag ttgctcgtac cgtagtagat aacgtcatca gtgaacaaat gggcttttat
    26161 ttgcaggaaa atagcgaaat gagccagatg ttggtccgta aagcaatcaa agccagagaa
    26221 gcgagagaag cagcacgtaa agccagagaa gaaagccgaa atgggaagaa gcgtaaaaaa
    26281 ggcgaatctc ttctttcagg aaaattgaca cccgcacaat cccgaaaccc taagaaaaat
    26341 gaattgtatt tagtcgaagg ggattctgct ggcggatctg ctaaacaagg aagagacaga
    26401 aagttccaag caattctacc tttacgagga aaagtcatca ataccgaaaa agctaaaatg
    26461 caagatatct tgaaaaatga agaaatcaat acgatgatct atacgattgg tgcaggagtc
    26521 ggtcctgagt tttctatcga agactgtaat tacgacaaag taatcatcat gaccgatgcg
    26581 gatacagacg gcgcccatat tcaagtatta ctgcttactt tcttttatcg ttatatgaaa
    26641 ccgctgattg aagctgggaa agtatatatc gcgttaccgc ctttatataa agtatccaaa
    26701 ggacaaggaa agaagcaagt cattgaatat gcgtggacag atgacgaatt ggcagcaatg
    26761 attaaaaaag tcggcaaagg atatatgctg cagcgatata aaggacttgg agagatgaat
    26821 gcggaacaat tgtgggaaac aacgatggat ccgacttccc gtacgttgat ccgcgtccgt
    26881 atcgatgatg ctgcgcaagc tgaacgtcga gtaaccacac tgatgggtga caaagtagaa
    26941 cctcgaagaa aatggatcga aaatcacgtg caatttacgc tcgaagaaga tggcagtatc
    27001 ttagataaaa aagaggatac tgaaatctct ccttcagttt ctaatgattt attagatgaa
    27061 gaacgagcag ataaaaatga aaacaatcag ttatttgaag tagaatagga ggtcacctga
    27121 tggaaaatcg acaagaagta caagaattaa ctttagaaga agtgatgggt gaccgcttcg
    27181 gacgttattc caaatatatt atccaagaac gtgcactgcc tgatatccgt gatggattga
    27241 agccagtgca acgccggata ttatttgcca tgaacaaaga tggcaatacg tatgacaaag
    27301 gattccgtaa atcagcaaaa tctgtcggaa atatcatggg gaattatcac cctcatggtg
    27361 accgcagtat ttatgaagcg atggtccgca tgagccaaga ctggaagttg cgggaagtac
    27421 tgatcgaaat gcacgggaat aacgggagta tggacggtga tccacctgct gcgatgcgtt
    27481 atactgaagc cagattatcc aagttaagtg gagagatgct agcagatatc gagaaagaaa
    27541 cagtcgatct cgtgtggaat tttgatgata cagaaaaaga accaacagtc cttccagctc
    27601 gatacccaaa ccttctcgtc aatggatcga caggtatatc tgctggatat gcaacagaaa
    27661 tcccgactca taacttagct gaagtgatcg atggcacaat ctatatgatc gatcatccac
    27721 aagcgagcct tgaaaaattg atggaatata ttcctggacc tgactttcca acaggaggga
    27781 tcttgcaagg aaaagatgaa atcaaaaaag cttatgaaac aggacgagga aaagttattc
    27841 ttcgttcgaa aacaaagata gaggcaatga agggtaataa acagcaaatc gtgatcacag
    27901 aaatcccata tgaagtaaat aaagcgacct tggtgaaaaa aatggatgag atccgtttga
    27961 ataagaaaat cgatggaata gctgaagtac gagatgaaag tgatagaaca ggtctgcaaa
    28021 ttgtcgtcga attgaaaaaa gacgccaatg ctcaaggaat tttgaattat ttgttcaaaa
    28081 acactgagct acagatcaat tacaatttca atatggtcgc tatcgaccat atgactccgc
    28141 atcaagttgg actgaaagac attttaagaa gttatatcga gcatcgaaag caagtcataa
    28201 caaaacgtag tcaattcgat ttagccaaag ctcaaaaaag acagcatatc gtagaaggat
    28261 tgatgaaagc tttatcgatt ctggatgaag taatcgcaac gattcgtgaa agtaaagaca
    28321 aaaaagacgc caaaaagaat ttagtcgacg tctttcaatt tacagaagaa caagcagaag
    28381 caattgtcac attgcaatta taccgtttga cgaacacgga tatcacagag cttcaaaagg
    28441 aatccgaaag cttgattgcg caaatcacag agttgaataa aatcctttca aatgacaaag
    28501 agcttttctc tgttatgaaa aaagaattgc gagaagtgaa aaaaaattat tcttccgctc
    28561 gtctaacgac aatcgaagac gaaatcgaag aaatcaaaat tgatacgcaa gtcttagtgg
    28621 cacaagaaga cgtaattgtc agtgtgacgc gagaaggcta tgtgaagcga acaagccttc
    28681 gttcttatag tgcttccaaa ccagaagaaa tcgggatgcg agaaggcgac tacttgctat
    28741 actcaggtga attgagtaca ttggaccatg ttcttctgat cacaaataaa gcaaatgtga
    28801 tctaccgccc tgtccatgaa ctacccgatt tgaaatggaa agatgctggt gagcacattt
    28861 ctcaaacaat ctccaatctg tctgtagacg aatcgatttt agctgttttc ccatatcaaa
    28921 aaattcaagc agagaaaaca tttgtattca tcagcaagaa tggattgatc aagcaaacac
    28981 gaatgacaga gttcgaacca tggcgcacct ataagagccg tccgctcagc tgtatgaagc
    29041 taaaggcgga agaagatgaa ctgattgctg tgtatctgaa agaagatcag ccaggcttag
    29101 atgttttctt agtaacgcat caaggaatgg gtttgcgcta tcctttaaca gatgttcccg
    29161 ttgttggagc aaaggcagct ggagtgaagt ctatgaattt gaaagatgga gataggatta
    29221 tcaacgggct gcttgtgtta gcagaaggtg atacgccgat cgtaatcgtt actcagcgag
    29281 gagctgttaa acgcatgctt gctcaagaaa tcagccaaac ttcacgtgcg aagcggggag
    29341 tgacggtcat gcgtgagctg aaaaaaaatc cgcaccgtat tatctatatg tcggaaggaa
    29401 gatcaaaaga aataaccttg atgaatcaaa aagggcagca gttaacgatt gacccaactg
    29461 atttccctat aggtgatcga acatcgaatg gatcatttgc gatggatgag aaaaaaggtg
    29521 gagaagtcat cgaagttatc gatgctcctg agattttcat cgagtcgtaa ctgttgtagt
    29581 attttaatga agagacgcca tctgtattaa tacaggtggc gtctcttttt cattcatttt
    29641 taataaatcc ttatacgttt gcaaaaaaag atgaaagtgt tcacaatgct taaatgttgg
    29701 tatgtcaacg ttgatttaaa tatatgtaaa taaaacataa aaattgttaa ataaaaaaca
    29761 agaaaaccct tgcaaagata gggtgctgtg ttatactaat cacgtaaaaa ctgttacata
    29821 aaacagaaat ttaggaggtc catacaatgg agcaatggaa tggatttaaa ggtaccaaat
    29881 ggaaagaagg cgtagacact agagacttta ttcaaaataa ctacactgaa tatcgcggtg
    29941 atgacagctt tttggaaggc ccaacagaag caacagaaaa attatgggat gaattgcaaa
    30001 agttaaatga tatccaattc gagcgtaacg gtgtctatga tatggacaca gaaatcgtat
    30061 caactattac ttcacacgat gcaggctatt tggataaaga tttggaaaaa atcgtcggtc
    30121 tacagacaga caaacctttg aaacaagcat tcatgccgtt cggcggaatc aatatggcta
    30181 acaatgcgct aaaaagcaat ggctatacac caaacgatga attgacacat attttcactg
    30241 actggcgcaa aacacataat caaggtgtat ttgacgctta tactgctgaa atgcgtgctg
    30301 ctcgtaaaaa caaaatcatt acagggcttc cagacgctta cggtcgcgga cgtatcatcg
    30361 gtgactatcg tcgtgtggca ttgtatggaa tcgactattt gatggaacaa aagaaaaaag
    30421 atcatgatat gactggttat catacaatga gtgaagaagt gattcgttta cgtgaagaaa
    30481 tcactgaaca atatcgtgca ttagcgcaaa tgaaagaaat ggctgcgaaa tatggctatg
    30541 atatttctaa accagctgca aatgcaaaag aagctgtcca atggctatac ttcggatatt
    30601 tagcagcaat caaatcacaa aatggtgctg cgatgtcaat cggacgtatc tcagcattcc
    30661 ttgatatcta tatccaacgt gatttggata atggagtaat cacagaaaaa gaagcacaag
    30721 aattgatcga ccacttagta atgaaattac gtatggtcaa attcgcgcgt acacctgaat
    30781 ataaccaatt attctcagga ttccctatct gggctacttt atccattgca ggtatgggat
    30841 tagatggtcg ttcattagtt acaaagaatg atttccgtct gcttcataca ttaacaaata
    30901 tgggaccttc accagaacca aacttgactg ttttgtattc tgaacacctt ccagaaggat
    30961 tcagaacata tgctagcaga atcgctatcg aaagttcttc gattcaattc gaaaatgata
    31021 atcagcttcg tgaacaatgg ggttctgatg attgtgcgat tgcttgctgt gtatcaggaa
    31081 cagttgtcgg aaaagacatg caattctttg gcgctcgtgc taacttggct aaaacactat
    31141 tatatgccat caatggcgga gtcgatgaag tgactggcgc ccaagttgga ccagaatatc
    31201 gtccaatcac atcagaaata ctagactttg aagaagtgaa acataacttc atgaatatga
    31261 tggattggtt agcagaacta tatgtcaata cgttgaacat cattcattat atgcatgata
    31321 aatattctta tgaagcagct cagctggctt tgatggattc tgacttgcaa cgcacatttg
    31381 ctacaggtat tgctgggatc tctcatgcgg cagacagctt gtcagcgatc aaatatgcaa
    31441 aagtaaaagc aatccgtaat gaaaaaggaa tagcagttga ttttgaagtg gatggcgact
    31501 tccctaaata cggaaatgat gatgaccgtg cagatgaatt agtttcatgg atcttggaat
    31561 acttcatggg acaaatcaaa cgtcataaaa cttaccgcaa ctcaaaacca acaacttcat
    31621 tattgacgat tacttcaaat gttgtttacg gtaaaaatac aggaaatact cctgatggcc
    31681 gtgaagctgg aaaaccactt gcgccaggag caaatccaag ttacaatgcg gaacaaagtg
    31741 ggttattagc tagcctgaac tcaacagcta aaatgaatta cggctgtgca ttggatggaa
    31801 tttcaaatac ccaaacaatc aatccttcag gtcttggaaa agaacaagat acaagaatcg
    31861 aaaacttgcg taatgtctta gatggatact tctcaaaagg agcttatcat ttgaatgtca
    31921 atgtattcag taatgattta ttgcgtgatg caatggaaaa tccaatgaaa tatccgaact
    31981 tgacgatccg ggtatctggt tatgcagtta aattccgtga cttgacaaga gaacaacaat
    32041 tagatgtctt gatgagaact tcacacgaca gaatgtaaaa agttcgtgaa aaaagttttt
    32101 cgacttagcg tcgaaaggac gttgaatagc catcgcatca aaatcgaata atcagtatgc
    32161 ttatttgaaa gaaacgattt acaggggcag gacttgtatg ttccgtccct gtaaatttta
    32221 cacataacga agcggcaatt gcgaaaaatt ttgttcagaa agcttcgctt cgacagctaa
    32281 cttgtactta aaatgaaatc aaagaaaaga tgtgaactta aatggaagaa aaaacaatcg
    32341 gctatgttca ttctatcgaa actttcggtt cagtagacgg acctggactg cgctttgtcg
    32401 tgtttatgca aggatgccgc atgcgttgcc aattttgtca caatccagat acttggaata
    32461 ttggcggagg gaaagaatac acagcagatg aacttttaga taaagcagaa cgcttcagac
    32521 cttactgggg agataaaggt ggtattactg ttagtggtgg agaacctcta ttgcagatcg
    32581 attttttgat cgaactattt aaaaaagcaa aagagcggaa gatgcatact acattggata
    32641 catgcggaaa accttttacg tatgaagaac ctttcttttc acgttttcaa gaattgatga
    32701 agtatacaga tttactgtta tttgacatca aacacatcga taatgaagag cataaaaaac
    32761 taacgcattg ggacaatgaa aatattttag aaatggctca atatttgtca aaaatcaaca
    32821 aaccggtttg gattcgccat gtactagttc ctgagcgaag tgattatgat gaatatttga
    32881 tccgactaga caactttatt caaaccttgt caaatgtaga cagagtagag attcttcctt
    32941 atcatacgat gggaaaatac aagtgggaaa cacttggttt gaaatatcca ttagaaggga
    33001 tcgaaccacc tacaaaagaa cgtgtcgaaa atgctaaacg attgcttcat acagaagagt
    33061 atacaaaata tttaacgcga taggtcgcat cggaaagaag ataagtattg cttctttccg
    33121 tttttttgct attattaaat aggagaaatc acaaaggagc gaatgacatg tcaaaaattt
    33181 tagtttttgg tcaccaaaat ccagatacag atgcaatcgg ggcagccatt gcgttttcac
    33241 acttgcaaaa tgaattaggt aaagaaacag aagcagttgc tctaggtaca cctaatgaag
    33301 aaacacaata cgcattagac cactttcagc tatctgcacc acgagtagta gagtcagcta
    33361 aagcagaaaa tgcagatgtc atgcttgtgg atcataatga attccaacaa agtatttcag
    33421 atatcagcga agtaaatatt ttagctgtag tagaccacca tcgtattgca aactttgaaa
    33481 cagctaatcc attgtactat cgtgcagaac cagttggatg tacaagtacg attattttaa
    33541 aactatataa agaaaaccaa gtaacaattc ctaaagaaat cgcaggaatc atgctctcag
    33601 ctatcatttc agatacattg ttgttcaaat caccaacatg tacgcctgaa gatgaagcag
    33661 ctgcaaaaga acttgcagca atcgctgaag tcgatctaaa cacttatgga cttgaaatgt
    33721 tgaaagcagg aacaaatcta agtgataaat cagctgcggt tcttcttgat ttggatgcaa
    33781 aaagtttccc aatgggcagt tccaacgttc gtatcgctca agtcaataca gtggacctta
    33841 atgaagtgat ggatcgacaa gcagagctag aagcagcaat ggaagatgaa atgaaagcaa
    33901 atggctacga tctgttcgta ttagttgtca caaacatctt agacagtgat tcagaattac
    33961 ttgttgctgg taaaggaaaa gaaaaagtag aagaagcttt ccaaacaact ttgaccaaca
    34021 accgtgcttt gcttaaaggt gtcgtttcac gtaaaaaaca agtcgtacct caattgacgg
    34081 ctgtatttag ctaatcaaat ggtagtatga agcattcaaa atgaatggat aagcgattga
    34141 tataaaaaga aacatagaac atactaaagg ccgaagaaat ggaggagaat aacagtcaat
    34201 tatgactctt gttcttcctc ctttttacgt aaaagccttt taagtaaaat aaaacggata
    34261 gaaaagagaa catatatgaa ctattattca aaaaataagg aaaactttta tcaaaaactg
    34321 accgatgatc cgcttttttc ttcattgacc gattatctat atgaacatcg agaacaagaa
    34381 acgattctaa gagaattgaa aaaagaattt tcgcaaaata aattttccca ttttcttgat
    34441 ttattgatcg atgcaggatt aatcaagagg gaagaacgaa gatatcattt gaactttcca
    34501 atatttgatc ctaaagatta cttgcaacaa gccacttcgg ctgctgaaac catagctgaa
    34561 caattgaaga gactctctgt tgatgaacaa aaattagcga tgggagaagt tatttgggcg
    34621 tattgtttcg aagatgaacg gaaagaagcg tatttttatg gagtaagaaa cagccgagag
    34681 acagaattgc tgaggacaac tgcaggaaat gagaagtacc gttttatcac actttcctct
    34741 atagaacact tcccgcttac tttggctaat tacttttttg tacagaagaa tcagcttcct
    34801 gtaacgaagg cattcaaaga attagccgaa cttattggag acgtaaatga atcgtacttt
    34861 tttgatcaaa tagaagttat cgttgacagg atacgtaaga ataaatataa aaatcgccgg
    34921 cctagtattt tccatcagtc tctacttgtg acgaatacta taaaggaaga agaatcattc
    34981 acattagaat tacctattgt cgaaaaaaac aattttgaga ttgaattacc aacattggat
    35041 ccttcattga caatggagga aatggccttt cttaaacgac aaatcttttc tgagctgagc
    35101 aagaaattta taccgcatgc attttcttat atcaaagaat atggaacagt tttagtgagt
    35161 aaaacctaag agagcagtaa gcaaaaatta tactgactct tgcaggagga gcctttctct
    35221 tcctttatgg ttaaaagaat tatttgagta ttttgcctat ctaccaccta ttagattcac
    35281 tacatggtct ggaaacaaag gaatgcaaaa aaatggtgag aaatgtggaa tgacaaagga
    35341 agtagtgata agaaaagtta gatttttaaa taatcaatat tatgattcag tcaagtatgg
    35401 cattttatgg gaagaactag ctgattagat taggaagtcc atttgataat ccagaatcac
    35461 taagaaacga atggcgagtc attaataatt tatccaatct tgggttattc ttttagattg
    35521 aagtgtttaa aactaaaaaa gcatttatga agtcgtaata gtcaggcttc aaaatgcttt
    35581 ttattttgtt tgttttttct ttgccacata aatacaccta gactgacgat gaagagcata
    35641 tccaacagat ttcctgcgac ttggatcgtc gaagggttgg tcagtggcat taaagcaagg
    35701 acaaaaaagg aaaacaagct gtaaataaaa caggtgacag gaactaatcg aaagaagagc
    35761 ggacatttca attggctaaa aatgagtgaa aaaataaaat aagtaaaaat ggataaaatc
    35821 acgactaaac cattcatgat aaagaagaaa agcgtattgt gattaaacga ataaaaaaga
    35881 attgctaaaa aagaaaacgt tttaaatgca atcaatacgc ttgaagcttc gtttaacgta
    35941 ccaggatgtc gatgaaaaaa ataggcaccg aaataaacag aaaacagtaa gccgcaaaga
    36001 gaagacaaaa tcaaaaaaaa cggattttct tcggcttctg tgatatcaga atgatccaaa
    36061 agcaaccagc ctaatgcact agaaggcgca tgtgaggtct taaggtatga ataacataga
    36121 aaaaataaga tcgtcaaagt tgcaaaaaaa gagcctaaat gtaaaaaatt agtggttcta
    36181 cgttgaattt ccataatatc tccaaattaa attgataagt atataaagta taataacaaa
    36241 ttgatgagta tatgtcgatg ttttttaatc aaaataataa ataaaaactg tctaacattt
    36301 aaaatttaat ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt
    36361 ctcacttttt tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa
    36421 gcacgctctt cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa
    36481 agtatttcga gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca
    36541 taaccgtgtt ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa
    36601 atcgttgtga ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg
    36661 atacctactg cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct
    36721 aaaacaaggg ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca
    36781 atcagtcgat agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca
    36841 aaattaatga ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc
    36901 atttatataa caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca
    36961 tacaaaaaaa tgataaaatg attataatag aattataaag ataggttagg tgattaaaat
    37021 gaaaacggtc aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata
    37081 cttaggtgtc tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc
    37141 gctgacagat ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat
    37201 tttcggtagc gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt
    37261 aggagtggtg ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat
    37321 attatttggt acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc
    37381 aggaaattta atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt
    37441 ccatcatatt ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt
    37501 tgtcatctat tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa
    37561 aattgcgcag cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa
    37621 acagccggtt tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt
    37681 tgaatttgac acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc
    37741 agttggcggg gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca
    37801 tatagtagcg ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat
    37861 cgtaaggcgc gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc
    37921 atgggttaag agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt
    37981 cttttggcat ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga
    38041 aaaaaatcgg cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg
    38101 acatatggta caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat
    38161 aaaaaagtat aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt
    38221 ttattttggg ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga
    38281 caacatgtta gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat
    38341 gtttcaaaaa tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg
    38401 aaagagttag ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa
    38461 gcacttgtca cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca
    38521 actgagccct attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat
    38581 tattctctcc aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc
    38641 atcacaggtg tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc
    38701 ttgtttgggg aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca
    38761 gcaaaagcaa cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc
    38821 gaagtggacg agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa
    38881 gttgatcttc ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat
    38941 atgacagaaa actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg
    39001 ctagcactag gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt
    39061 ggtgtgattg gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg
    39121 atcaagcaag atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata
    39181 gaagaaaaag gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag
    39241 acaacgaatt aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa
    39301 aataatcagc gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca
    39361 tttttgagaa ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg
    39421 tacgatgatg agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga
    39481 caagaagatc ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat
    39541 ttcacgaaca cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag
    39601 tcagctccgc gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc
    39661 cattaccaat cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt
    39721 tctgtcagac aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt
    39781 gacacgaagg tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc
    39841 ccagacccta gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg
    39901 gatgactctt atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt
    39961 gagccgtctg tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa
    40021 gaaaaaaaat cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat
    40081 gatcaaggaa actctttggg agaaaatagc tattttaaat aagtgatata caaatttata
    40141 ttcggctaca gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa
    40201 gtttatatcg tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa
    40261 gaaaataatg gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt
    40321 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
    40381 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
    40441 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
    40501 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
    40561 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
    40621 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
    40681 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
    40741 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
    40801 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
    40861 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
    40921 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
    40981 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
    41041 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
    41101 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
    41161 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
    41221 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
    41281 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
    41341 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
    41401 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
    41461 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
    41521 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
    41581 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
    41641 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
    41701 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
    41761 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
    41821 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
    41881 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
    41941 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
    42001 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
    42061 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
    42121 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
    42181 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
    42241 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
    42301 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
    42361 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
    42421 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
    42481 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
    42541 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
    42601 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
    42661 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
    42721 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
    42781 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
    42841 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
    42901 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
    42961 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
    43021 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
    43081 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
    43141 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
    43201 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
    43261 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
    43321 taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc aagtcaatgt
    43381 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
    43441 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
    43501 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
    43561 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
    43621 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
    43681 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
    43741 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
    43801 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
    43861 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
    43921 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
    43981 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
    44041 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
    44101 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
    44161 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
    44221 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
    44281 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
    44341 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
    44401 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
    44461 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
    44521 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
    44581 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
    44641 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
    44701 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
    44761 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
    44821 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
    44881 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
    44941 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
    45001 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
    45061 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
    45121 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
    45181 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
    45241 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
    45301 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
    45361 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
    45421 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
    45481 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
    45541 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
    45601 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
    45661 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
    45721 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
    45781 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
    45841 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
    45901 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
    45961 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
    46021 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
    46081 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
    46141 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
    46201 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
    46261 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
    46321 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
    46381 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
    46441 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
    46501 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
    46561 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
    46621 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
    46681 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
    46741 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
    46801 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
    46861 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
    46921 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
    46981 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
    47041 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
    47101 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
    47161 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
    47221 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
    47281 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
    47341 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
    47401 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
    47461 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
    47521 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
    47581 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
    47641 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
    47701 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
    47761 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
    47821 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
    47881 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
    47941 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
    48001 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
    48061 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
    48121 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
    48181 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
    48241 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
    48301 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
    48361 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
    48421 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
    48481 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
    48541 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
    48601 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
    48661 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
    48721 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
    48781 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
    48841 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
    48901 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
    48961 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
    49021 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
    49081 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
    49141 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
    49201 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
    49261 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
    49321 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
    49381 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
    49441 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
    49501 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
    49561 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
    49621 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    49681 ta
//
LOCUS       DACEDD010000016        46324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEDD010000016 DACEDD010000000
VERSION     DACEDD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.016"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..519)
                     /locus_tag="IWO45_001055"
     CDS             complement(<1..519)
                     /locus_tag="IWO45_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0332542.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
     gene            730..1176
                     /gene="nrdI"
                     /locus_tag="IWO45_001056"
     CDS             730..1176
                     /gene="nrdI"
                     /locus_tag="IWO45_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0332543.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(1246..3228)
                     /locus_tag="IWO45_001057"
     CDS             complement(1246..3228)
                     /locus_tag="IWO45_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0332544.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(3242..4138)
                     /gene="murQ"
                     /locus_tag="IWO45_001058"
     CDS             complement(3242..4138)
                     /gene="murQ"
                     /locus_tag="IWO45_001058"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0332545.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(4141..5202)
                     /locus_tag="IWO45_001059"
     CDS             complement(4141..5202)
                     /locus_tag="IWO45_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0332546.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(5414..5941)
                     /locus_tag="IWO45_001060"
     CDS             complement(5414..5941)
                     /locus_tag="IWO45_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0332547.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(6008..7510)
                     /gene="lsa"
                     /locus_tag="IWO45_001061"
     CDS             complement(6008..7510)
                     /gene="lsa"
                     /locus_tag="IWO45_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0332548.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(7768..8889)
                     /locus_tag="IWO45_001062"
     CDS             complement(7768..8889)
                     /locus_tag="IWO45_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0332549.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            9015..9956
                     /locus_tag="IWO45_001063"
     CDS             9015..9956
                     /locus_tag="IWO45_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0332550.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(10099..10995)
                     /locus_tag="IWO45_001064"
     CDS             complement(10099..10995)
                     /locus_tag="IWO45_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0332551.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            11277..12617
                     /locus_tag="IWO45_001065"
     CDS             11277..12617
                     /locus_tag="IWO45_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0332552.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12812..13255
                     /locus_tag="IWO45_001066"
     CDS             12812..13255
                     /locus_tag="IWO45_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0332553.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13517..14935)
                     /gene="nhaC"
                     /locus_tag="IWO45_001067"
     CDS             complement(13517..14935)
                     /gene="nhaC"
                     /locus_tag="IWO45_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0332554.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(15339..16562)
                     /locus_tag="IWO45_001068"
     CDS             complement(15339..16562)
                     /locus_tag="IWO45_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0332555.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16594..17433)
                     /locus_tag="IWO45_001069"
     CDS             complement(16594..17433)
                     /locus_tag="IWO45_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0332556.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17450..18130)
                     /locus_tag="IWO45_001070"
     CDS             complement(17450..18130)
                     /locus_tag="IWO45_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332557.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(18132..19205)
                     /locus_tag="IWO45_001071"
     CDS             complement(18132..19205)
                     /locus_tag="IWO45_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0332558.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(19855..20526)
                     /locus_tag="IWO45_001072"
     CDS             complement(19855..20526)
                     /locus_tag="IWO45_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0332559.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(20614..23292)
                     /gene="mgtA"
                     /locus_tag="IWO45_001073"
     CDS             complement(20614..23292)
                     /gene="mgtA"
                     /locus_tag="IWO45_001073"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0332560.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            23952..24389
                     /gene="ndk"
                     /locus_tag="IWO45_001074"
     CDS             23952..24389
                     /gene="ndk"
                     /locus_tag="IWO45_001074"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0332561.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(24411..25277)
                     /locus_tag="IWO45_001075"
     CDS             complement(24411..25277)
                     /locus_tag="IWO45_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332562.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(25511..27589)
                     /locus_tag="IWO45_001076"
     CDS             complement(25511..27589)
                     /locus_tag="IWO45_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0332563.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            28082..29299
                     /locus_tag="IWO45_001077"
     CDS             28082..29299
                     /locus_tag="IWO45_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0332564.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(29758..30381)
                     /locus_tag="IWO45_001078"
     CDS             complement(29758..30381)
                     /locus_tag="IWO45_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332565.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(30537..31934)
                     /locus_tag="IWO45_001079"
     CDS             complement(30537..31934)
                     /locus_tag="IWO45_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0332566.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(31956..32927)
                     /locus_tag="IWO45_001080"
     CDS             complement(31956..32927)
                     /locus_tag="IWO45_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0332567.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(32934..35234)
                     /locus_tag="IWO45_001081"
     CDS             complement(32934..35234)
                     /locus_tag="IWO45_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAQ0332568.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(35236..36561)
                     /locus_tag="IWO45_001082"
     CDS             complement(35236..36561)
                     /locus_tag="IWO45_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0332569.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            36792..37217
                     /locus_tag="IWO45_001083"
     CDS             36792..37217
                     /locus_tag="IWO45_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332570.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(37715..38416)
                     /locus_tag="IWO45_001084"
     CDS             complement(37715..38416)
                     /locus_tag="IWO45_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0332571.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(38432..38872)
                     /locus_tag="IWO45_001085"
     CDS             complement(38432..38872)
                     /locus_tag="IWO45_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAQ0332572.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(38872..39447)
                     /locus_tag="IWO45_001086"
     CDS             complement(38872..39447)
                     /locus_tag="IWO45_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0332573.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39858..40364)
                     /locus_tag="IWO45_001087"
     CDS             complement(39858..40364)
                     /locus_tag="IWO45_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAQ0332574.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(40375..40854)
                     /locus_tag="IWO45_001088"
                     /pseudo
     CDS             complement(40375..40854)
                     /locus_tag="IWO45_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(41026..41727)
                     /locus_tag="IWO45_001089"
     CDS             complement(41026..41727)
                     /locus_tag="IWO45_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0332575.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(41835..43244)
                     /locus_tag="IWO45_001090"
     CDS             complement(41835..43244)
                     /locus_tag="IWO45_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0332576.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(43292..45157)
                     /locus_tag="IWO45_001091"
     CDS             complement(43292..45157)
                     /locus_tag="IWO45_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0332577.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(45278..46129)
                     /locus_tag="IWO45_001092"
     CDS             complement(45278..46129)
                     /locus_tag="IWO45_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0332578.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT        13350 a         9948 c         7979 g        15047 t
ORIGIN      
        1 agctactaca ataaataaaa ataatggcac taatttacaa aatgtgacga tagcattgac
       61 aaagctagca ttttcaactc cacgattcac tagccatgca tatccccata agaagatcgt
      121 actcatcacg atcgatgcca cattttgacc accttcgaat acggggaaga aataacctaa
      181 tgcactcatc aacaatgttg cgaacgccac gtttcctaac cagcatgtga gccagtaacc
      241 ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc
      301 gccttttaaa tctggacgtt ttttattgat attattgatc gataaggaga gcatcaacat
      361 tgctgttcct acgagtaccc agctgaagat agctgggcca ccagccgatc cggagatgtc
      421 cgtcataata ccaaatacgc cacctccaat cgctgaacca atcactaagg cagcaagtgg
      481 aatcaaacca attccttttt tctgttgctc tgtatccatg taagcatcct ccaaagtaaa
      541 accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt
      601 taatagaaat ataattctac cgactttttt ctcattatac aagcaaagcg cctagtccct
      661 ttccaaatta caccaccaaa aaaacttaaa aagatgctat actaaaggga aagaattggg
      721 ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc ttttgccaaa
      781 catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact
      841 ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc
      901 gttcctactt atttagacgg cgggaatggg ttagacaacg gtgacacaga aatcttgacc
      961 gaaacgatgc gcgaatatct ggaatacgaa gacaatcata aactttgcct tggtgtagtc
     1021 ggtagcggta ataaaaactt caacaatcaa tattgtctga cagccaaaca atacgcacaa
     1081 agattcggct ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca
     1141 cgcgtttatc aaattttggc agaaaaaaat aaataacaca aaagaagaag ctgaaaaata
     1201 aatttcggct tcttcttttt atgttctcta ttgtttctat tcttattaag ctttgacatc
     1261 catcacaggt gcttttgctt tcgctttacc tgttttttcc aagacaaagt tttttacacg
     1321 atccatattg gttaccaaga cgatcatgga ggtttctttg tctgctgctt tgattgcttc
     1381 taagtcagct tctgctacaa gcgtatcagg tgtcacttgc tgtccttctg ttactttcac
     1441 atcgaatggc gtaccgccta agtcaactgt gttgatgccc atgtgaagca agatttctaa
     1501 gccatttggt gtcgtaatgc cgatagcatg ttttgttggg aaaacagaac ttattactcc
     1561 agtaaccggg gaatagatat gtccgttttc tggttctaca aagtaacctt cacccatcat
     1621 tttttgtgca aaaactgggt cacttgcttt atctaaagaa acaactgttc cgtcagcaac
     1681 tgatgataag atagtcacac tttcagctga agcttgaggt actgcttttt caggtacttc
     1741 atttactgtg gctgtttcgg tcgctagttc ttctttttct aaacggtctt taggaatacc
     1801 gaagaagaat gtagcaacga agccaccagc gtaagcagcc aataaaccaa ggatatagcc
     1861 taaccattta ccatcagaga tcaatggaat caaagcggct cctgaaggtc caatagcgat
     1921 tgctccgaca tttccgatca ttccgatcac tgccccaccg atacctccac cgatacaagc
     1981 agtaataaat ggacgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc
     2041 taagattccg actggtaaag cccctttgat catttctgtt aattctttgt cttttttgca
     2101 gcggatccat aatgccaatg ctgcacccac ttgtcctgct ccggccatcg caaggattgg
     2161 aagcaataag gtacgaccag tttcatcaat catttgtaca tggattggcg tcaagatctg
     2221 atgtagtccg aacatgacca ttggcaagaa taatgtacct agaacgaagc cggcaaatgc
     2281 tcctccaaca ccaagtaccc agttgatccc accgaccatg ccgtcagaaa tgaaccctgc
     2341 taaaggcatg atgaagaaaa tttcgatcag accgatggca atcagggaaa gagttggcgt
     2401 aacgatgata tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa
     2461 taaccaaact gcgaagataa cacctaagat cccgccttgt ccagttgcaa gagcttcacc
     2521 ggtaaacaag tttttcagtg gtgcttctgg attcatccct gtcagcatca caacagcccc
     2581 gataactcca ccaagcgttg gcgttgcacc aaaaacttgt gcagcattga tacctgtata
     2641 gataactaga tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg
     2701 ccatgttcct gcgtctagtg ttccagctgt gactaagttt gctaaaactg cggcaatccc
     2761 tgccacgatc ccagtgccga caaatgcagg gatcattggt acaaagatat tagagatgtc
     2821 ttttaatacc gatttgattt tagagggttt ttgtttcgat ttttgtgccg ctttcatttc
     2881 ttgtgcccgt tcgtttacac ggtcttttcc agatgcagaa gaacttgctg tagttgcact
     2941 agcagaagga agtttttctc ctaatttcac gcctgctaag tcaaccattt cctgcgctac
     3001 tttgttcact ttcccaggtc cgatgatgac ttggagctgt tcatcctcta ctacgccaag
     3061 tactcctgga atcgctttta attccgagac atttacaaga tcatcattga tgaccttcat
     3121 acgtacacga gtcatgcagt ggacaatgct ttcaacattg cccataccgc ctgctccttg
     3181 ataaatttgt tttgccagtt cttccaaagt caattcgttt tttgccatgg tcttttcccc
     3241 ctcataaggt tccttttaca aaaccgtttg cttcagctaa ttttgcttga gctgcctctt
     3301 tatcactatt tgtcaaaatc atgacgatag ccagtttgac attttgatct gcttcctcaa
     3361 agtacttttc agctgtttcg tagtcagctt ctgtcgcttg catgatgatt cgttttgagc
     3421 gttcaattag tttttcattt gttggtttga cgtcgaccat caagttattg aatactttac
     3481 cgataccgat catactgata gtagaaatca tattcaaaat cagtttttgt gctgttcctg
     3541 acttcaatcg agtagagcct gtcaaaaatt ctggtcctgc atcgacttcg attgggaaat
     3601 ccgcatgctc gctgatttct gcttgtttat tgcaagaaat cgttcctgta acagcaccaa
     3661 tcgattttgc gtaatctaat ccaccaatca catatggtgt acgaccgcta gctgcgatac
     3721 caataactat atcatttttt gttaaattca gcttgattag atcttcttgc cctaattcat
     3781 aagaatcttc ggctccttcg acggctacag tcattgcttc ttctccacca gcaattaggc
     3841 ctatgaccat ttccggttct acaccgaatg tcggtacaca ttcagcagca tctaatacac
     3901 caagtctacc actcgttcca gctcccatat aaatcaagcg accacctttt ttaaaggatt
     3961 caatcgtgtt ggcgatcact ggctcgatac ttggcagttc tttttcaaca gcagcggcca
     4021 ctttctgatc ttctttgttc atgagttgga ctgcttgaga aatactcatt tcatctaatc
     4081 caaaagtttc ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt
     4141 ttatcttcct tcttttctta taaattgata gttcatacca gctttgattt ggttgatcag
     4201 cggtagttct tctttgatga ctttggctac tacattcact ttttcatccg ctggcaaatc
     4261 atatttagtt aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat
     4321 agatcctttc atacgcttag ctgtatttct tactgggatt ggcggaatct ctcgaaaacg
     4381 cgcatctgcg cttcgtatga catctcttgc atcatcttgc cgattcgtat gttttcctaa
     4441 tatatattcg taaaaaactt catctacagg ttccgtatgg agcaagactg tctgttcttt
     4501 ttggaaagaa gcaaattgct cctgaacgtg ttctgacaga cctgcatctc cgatataaac
     4561 aatatccgtg ttacttgcta atagatcaag agcacctgcc agaggatgga tccctcgatg
     4621 ttcttccaaa gttggtaatc cttgatacag tggacctctc aactctgtat ctcctggtac
     4681 aaagccttgt acaaggaatc cttgagtttt gagccattga tttttttcct gataccattc
     4741 tttgtccaac cctgtttcaa gacgtggata ataattatgc caagcttcaa tctgtgagaa
     4801 atcagccgct gcttctttca actcatcgat atcattaggc gtgatcgtgc tggcattcaa
     4861 actgattttc aattgatgtg accagtcggc gatctgctga ttagaaatat gataatccat
     4921 tcttaaccct gttacaccaa ttgctttcaa gggtgttaga tcttctaatg aaaagccagc
     4981 tcgtttcaat gcctctcctg aaatatcgac catcaactct agtttattct ttttcgcttc
     5041 gcttcctagt tcgatcaatc gattttgata agccgaaaca tcatcttccg gtatatgcat
     5101 ggaagtgaaa ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc
     5161 ttctgacaaa gaatcattca taaatattga aaagccaaac atattatccc tctttttctg
     5221 tatccgtctt catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt
     5281 tgttaaaata attaaatttt atttctaaaa attggattta taatgccaaa agaattgaca
     5341 cagccacaaa aaaggcactg ctaagcaaaa gcagtgcctt gaaacatttt atttaaataa
     5401 cacaaaagtc taattactca taagaaatta attctgtatt gtctaccatg aatgtaaaga
     5461 acccttcatt aggcattgga tgaaaataag cattgatcac tcttatcaag gcattatgac
     5521 agactaacaa gtagacattt tcttcgtctt ccatgcattc tttcaataaa ggaacgatcc
     5581 gtgcgtacac atctaagacc gactctccat ttgggaaacg tacagcaaac gctaaacgcg
     5641 ctttttgaaa gttttcatct ttgcttggca gtccgtcata atccccaaaa tccatttcta
     5701 ccaatcgctc atcagcggtc atcggcaaat tgagacgatc aactactgct tgtgctgtat
     5761 ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat tggaacaggc aaatctacta
     5821 ccttttctgc tagtgcctgt gcctgcatct gtcctttttc agtcagcggc acatctgctc
     5881 gcccgcaaac taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt acataaagca
     5941 tgtgattctc tccttcgttg acaaagttct ccttaaccat accttgttgt ctttgagatt
     6001 acaaggttta gattttctca agagaaataa tctcagtaga tactttatct agaaaggttt
     6061 gatcatgttc cactagtaac atagcaggtt tcacgttcaa aatcagttgt tctaattgtt
     6121 cttgattgaa gacatccaaa taattcaatg gttcatccca tatatagagc tcagctggtt
     6181 gggataaaga tttagccaat tccacttttt tccgttgtcc catactcatc tgttcgattt
     6241 tattatgaaa aacatctctt tccatcccaa gctttcggag attattcaaa aatgcttgat
     6301 agtctactcg gtttttcttc gcgaattccg ccaatgtccc tcgattgtct tcataatttt
     6361 ggcgtacgta actaatggtc aaatgtttgg gctgggattt ttctcctaca accttgccgt
     6421 tgaatgtccc cagaagataa tggataatgg atgactttcc cgcgccgtta ggacctgaaa
     6481 ttgccatccg ctgatgaggt tcgatcgtaa aatgaactgg ctcgaataac agattttcat
     6541 accctaattg aagattttct acgcttaaca gccgtttatg atgagacact tggctattca
     6601 tcgtcaaagc atcgatatac tcgatatctt ttaataattt ttctttctca ctgatctggg
     6661 tttccatccg attcacgatt gctttagatc gtttcatcgt ccgtgcagca tcagcgccta
     6721 ctgctccttt attcactcgc ctagattccg tatcgatgaa tccgactttt ttctttgttt
     6781 tgtccccttc tcgggaacga gaccattctg ctttttcagc tgctgttttc tttagcctgc
     6841 tgacttcttt tttcaatttt tcatttttag ctatttcaaa ttcatcacga agttttttct
     6901 gttcttcata gatagagaaa ttcccttgat aaagttccag ttggctcttt tcgattgcta
     6961 aaacatggtc cactacttca tcgataaatc cccgatcatg actcaccacg atgaatcctt
     7021 gttttttctt tttcaagtaa gccgctactt gttttctgcc agagatatcc aaatgattcg
     7081 ttggttcatc gattaacggg aaatgagtgt catccacaaa taagagtgcc agtaggactt
     7141 ttgtcttctc tcctcctgat agtgtgctga attctctcca taagatttca ggatctgttt
     7201 gcatcagttg gagctctctt tcaatttccc atatctcaaa atccataatc tcatttaaca
     7261 cataataggt caaacgctct ttatcttttg tttgctgcgg gaaatagaca aacgcttgct
     7321 gatggatcac ttgcccttga taagggagtt tgttttgcag gatattcagt aaagttgtct
     7381 ttcctcgacc gtttcgtccg ataagtccta gtttccattg tgtgtcaaag tttagattgg
     7441 cttgttcaaa taataatgtg ccttggctgt catagccgaa tgtcagattt tttatttcga
     7501 ttttagacat tattccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta
     7561 aagaggatgc aagggttggg gcacacaaaa agacccatac atcgaaagaa ccaagccaat
     7621 cccgaaaatc tgcacactgc tcccttgtct ccacaaggta cagtctgaat gcagctttca
     7681 tcaatcgact taatttttca atgcacggat ccctcacttt gtcctatatt tgaaattacg
     7741 atagcataaa gaaattgttt ttgtcaacta cttcgggaga atgttgaaaa atctgctgag
     7801 tgtctgtcct aagatttctg ttttttctaa tgtttgcctg tgttccattg cttcctctaa
     7861 atcgattggt cctaattgaa tgcttaagac aatggggaga aaatcaaaga gtccttccag
     7921 ctctcggtct ctacttccgc aaatagcgat tattggtttt cgatattttt ttgcaagcac
     7981 tgctacgcca tatggaactt ttccgtggat tgtttgctga tcaatccgcc cttctcctgt
     8041 aatgatgaca tccgcttgtt cgatctgttt ttctaatcca gtgatttggc tgaccaactc
     8101 aaaaccagag accatcttcg ctcctaacaa aaaaagggct ccgccaatac cgcctgctgc
     8161 tccgcttcct gggatgtcat ctaaatccac ctcccaaaca tcttttatct gctgagcaat
     8221 cttttcagct tgtttatcca agaaagctaa cgtcgtatcc gttcctcctt tttgtcttcc
     8281 aaattgccaa gcagctcctt gttgacctgt atatgggttg gtcacgtctg cagcgatgat
     8341 cacttttact ttagaaacaa gaacgttcct tcgatccaaa tgaagttttc tagtagataa
     8401 caaaggattt ccttctgtgt acccttctaa agaagcaccc aaagattgga gtaatcctag
     8461 accaccatcg ctacatccgc ttcctcctaa agtgacaatg atttgttcca cttcttgaag
     8521 cagtgtatct ttgataagct ctcccaaggc ataactagaa gcaagtcgga tggtttgatc
     8581 cgttggttta tcaagtaaat ctaatcctaa tacttgtgca gattcaatga ctgcggtatt
     8641 ttttccttgc cagttcgtca ataaataact gatttttcgc tgacgaaata agagatccat
     8701 cccatcaatc gtacgccagg ttcccccgag cgcgtaatag attgcttctg cagtattttc
     8761 tccgccatct gcaatcggca cattgatttt tcttccttta cggtcttccc aaccatttag
     8821 aactgcctga tttaattcaa atgaagaagc acttccttta aatgaatcga ttgccgccaa
     8881 aatatccatc tatatccctc actttcttct taagtaagac actttttttc acttttttca
     8941 aacaaaacaa tgctattttc ttctgttttt ctatatacta aaagggaact cattgaataa
     9001 aggagagaaa aaacatgaaa atcgcaatag ctggagccgg agcaatgggc agccgaatcg
     9061 gattaatgtt gcatcaaagc ggaaatgagg tcgtcttgat cgatcgctgg cctgctcaca
     9121 ttgaagccat tcgtacaaat ggattgattg ctgactttaa tggcaaagaa gtagttgcaa
     9181 aactgccaat ctattcacca gaagaaatca tcgaaagcaa caaacacgtg gatttgatcg
     9241 tagccttgac aaaagcgaat caattagatg atatgtttcg ttcgatccaa tccattatta
     9301 ctgagaatac ttatgttttg tgtttactga atggattagg tcatgaagat gtactggaaa
     9361 aatatgtacc aaagaaaaat atcttatttg ggatcacgat gtggacagct ggtctagctg
     9421 gtcccggaaa agtcacatta ttaggtgatg gagaaatcga actagagaac ttggaaccag
     9481 aaggcgaagc atttacaaaa aaagttgtag aagtctttca agaagcaaaa ttgaatccaa
     9541 tctatagtca taatgttcgt tattccattt ggcgaaaagc ttgtgtaaac ggaacattaa
     9601 atggattatg cacgatattg gattgtaata ttgctgaatt aggtgcgcaa aaagcggcag
     9661 agggcatggt tcgaacgatt gtatctgagt ttgcttctat cgcagcaaaa gaaggaatca
     9721 cattagatca agaagaagtg taccaacata ttgcttctac ttatgatcct gacaacatcg
     9781 ggttgcatta cccatctatg taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg
     9841 actacatcaa tggtgccgtt tggcgaaaag gacaaaaata tgatattgct acaccatatt
     9901 gtgccttttt gacccaatta gtccatgcaa aagaaggaat ccttggcgca gaataaaagg
     9961 ctctctgatg agaatcagct ggatcagcag ctggttctca tttttttacc cccatgaaag
    10021 ttcaaaaaga aaaacacaat tctcatgttg ccataagaat tgcgcttgtt tattttcatc
    10081 tatgattggt tggcactttt atttattttt aacagaggaa agatcgtatt catattgttc
    10141 ataaatagcg gagtcagtag tgaaaaaacg ttttgcccaa ttttctgcat caaaaaattc
    10201 caacagttct gatgggattg gttgaaatgg cgtatgcaaa aattccggta attcatctaa
    10261 atcacgttct tgaagcaaaa aaatattttc agaatgataa aactgataat gagctatggc
    10321 ttgattatct gtcactagtt tcttttgaaa atacatagat tcaaacgttc gcaaagtcac
    10381 tccttgctgc ccttcccgca acagttctag aatccccctc gattccccgg tttttttcag
    10441 atattcttca taagtaaggt atccaggttg ttcatttcct cgaagcggaa gaataaagta
    10501 attggtcgta acacctcttt ttgtaaattc ctgcttatat ttttctgccc gttcttttct
    10561 gccgttattt gttgcaccaa aaaatagatc cgtctttggt tcgctagaag aaaaatccat
    10621 atgtttcgaa taaaaggaac tattatgttt tagtccaagt tctttcgctt ctaaaaaatc
    10681 aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg
    10741 ttctgcttta aagtggttcc aaaaaaataa cacgattttt gcattggtaa atggacgcac
    10801 gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct gataatttgc
    10861 caactgttgt cgagctgatt taatgaattc cgactgtagt aaatcttttc cggaccaaac
    10921 atctaaagag cggatggaag gaaaaaaata aagtgattta tttttcgcaa ttagcaatac
    10981 gtcgtttttg ttcatctgtt ccgcctcact ttctctgtca ctttttccta tgatagcctg
    11041 tttaaatagt aaaaagccag taaaacgctt cctattattt ttataaaaat ttaacatttc
    11101 tatgaaaata cgatgaaata aatcattctt ccattaaaat atgcccaaaa gttttctgaa
    11161 tagttgtaat tatctgaaaa tttgttatca ttagtcatta ttattattac tatttattct
    11221 tactatttat tcttactatg cattttgatt gtgaatttaa aagaaagagg ttatttatgg
    11281 aagataaaaa attgaaaaaa caacttagct cccgacatat caccatgcta gctttaggtg
    11341 gagcgattgg tgcaggtcta tttaaaggaa gtggcgaagc gattggaatc gccggccctt
    11401 ctgtattgat cgcttttttg attggcggca taattttata tatcgtgatg aaaggtctgg
    11461 gaaaaattgt tttaagtggt ggtgatacgc accacggatt atctggattg attcgtccat
    11521 atctaggtgc tcatgctgcc gattttaccg attgggtcta ctggtccatg tggattatca
    11581 atatcattgc tgaagctgtt gcagctgctt cctttttaca gttgtggttc ccccacatcc
    11641 cagcttgggt atttgttttt atgttggctg ttttaactac tatcatcaat ctttattcgg
    11701 tacgattatt cgcagagaca gaatattggc tggcctttgc taaaatcagt gtcattatcc
    11761 tattgatcat ttttgctgct tatcttgtcg gtcagcaaat gttgggtact ggtgttttcc
    11821 ctacccttca acaattaact gatcatggtg gttttactcc ccacggtatg aagggaatca
    11881 tcagttcttt gcttgtagtt atctattctt acggcggttc tgagttgatt gctatcacag
    11941 taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggc gtcatgggaa
    12001 gaatcatttc cttttacatt attcctttat ttttgctttt gattattttt ccttggaata
    12061 ctttagcagg cacgaacgtt agtccttttg tgatggtatt tgaaaaaatg aatattccat
    12121 ttgcggcaga tatcgtcaat ttcgtgattg ttcttgctct gttctcgtct atcaattcag
    12181 gtgtatatgc ttcttcgaga attttgttct tccgcttgaa agatcggaaa ggttcctcta
    12241 gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtgctg ttttgtacca
    12301 gtgttcttta tctaggtgtg gctctttctt attttgtcgg tgacaaatta tttggctatc
    12361 tggcaggttc tctttcctat acagtattat tgatctggat cattatcagt gctgcgtcct
    12421 ttgtactgac actaaggaaa ggaacgttat gggacaaagg aatcagcttt tttgcattag
    12481 cagtcttatg gttgattttc cttggtatcc tattcacgaa ctccattggg gtaactgtat
    12541 tgaccggttt gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgctttcg
    12601 tcttgacgaa tgaatgaaac caatagagaa ttttattcta tttatattga atatctggag
    12661 ctattgaaaa aaaggagctg cgacaaaaat tgttgcagtt cttttttccg aataaacggt
    12721 gttcaaaaac catctgtgca gattaattga ttgttctcca cgctcatgac atgatagaag
    12781 aaaggaatga agaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat
    12841 gattggtgtt tcgacggtag tcatgtttgg tatgatgtat ttgaatgttt atgcgcttga
    12901 tcatatcttc ttcagtcgca cacgtgtatt catggcgttg atgatgggtg cagtcatggc
    12961 agtcatcatg ttgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgctatgat
    13021 tttagccgct agtcttcttg tttttgccgg ttccttattt atggtacgaa gccaaacaac
    13081 tgtcaacgat acggcgtgga tgaaggcgat gatccctcat cactctattg ctatcttgac
    13141 aagtaaacga gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca
    13201 ggcacaagaa gaagaaatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt
    13261 atccatccaa ataaaaaaag aggatgttcc agaagtgatc aacttcaaga aataagaaga
    13321 agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa gctgccctgc atcaagtaat
    13381 aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct tattaccgca
    13441 aatgcaagct tttgaacacc gtttattcgt aaaagggagt gtgacaaaag tcttgtcaca
    13501 ctcccttttt acgtttttat tgttctaatt gcgctgtctt gcttactgct tcttgcggtt
    13561 tcacaaattt ctttgagatc ccccaacctg taaaggcact gaccatcgaa aacacaggtg
    13621 aaagcaaact gaaaaacacg aatggcaagt aagtgagtgt aggaatgcca aatgtacttg
    13681 ctacgaaact tccggcgatt ccccaaggca ctaagtagtt gattactgtt ccaccatctt
    13741 ctagtgttct ccctaataca gttggatcaa atcctgcttt ttcgtaagtt tctttaaatg
    13801 cattaccagg caggataaca gatagaaatt gttctccaat aaagatattt actccgatgc
    13861 ttgtcatcaa tgttgtaaaa atcaattttg gggtgttcgt catctttctt gatactcgtt
    13921 ccataacggt tgagatcaac ccaaattcga tcaacaaacc acctaatgac aatgttagaa
    13981 taatcaatga cacagttggc atcatgcttt cgattcctcc acggctaagt agtgaatcga
    14041 tttgttggtt tccagtattt gaaacaaatc ccgattcgat gacccctgcc aacttcgtta
    14101 aggaagtttg tgggttttga ataaagatca ttcctactgc tacagcaata ttcaacaaaa
    14161 tcgtcaaaat tgccggtacc tttttccatg cacacaagag cattaaaaat agaggaatca
    14221 gtgaccagat cgaaatagta aagtttgctt ctaaaatatc agtcacttca cgaatagatg
    14281 aaagacttgc ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa
    14341 atgccggaac agttgaccac atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa
    14401 tagctgctgc taaatttgtt gattcggata atggagacat cttgtctcca aaaacggcac
    14461 ctgaaacaat ggctccaaca acaagggctg gatttagtcc tagtgttgtc ccaataccga
    14521 aaaatgcaat cccaatcgtt gacataacag taaaggcact ccctacagcg ctacctacga
    14581 ttgcacaaac aacaaataca gaagggacga accactggac actaatcaat ttaaatccaa
    14641 tgaccatgag ggttggaata attcctgctt gtatccatac agcaatcaat gaaccgacta
    14701 aaataaaaat aaagattgga atgatccccg gtttgatgcc attgatgatc ccttcgtgca
    14761 gccaatctgt cgggtatttt tttatcactg cgtacaacat gaccattccg atagctgcca
    14821 aaatcgtgat attaggtgaa acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa
    14881 tgaccatcaa tacaagtgtt gcttctaaaa aacttggtgt tccttttttt tccatgataa
    14941 tctctccttt tttagttgtt cttatgttct aaaaaagtta agatatcgga ttactccgcc
    15001 acaattcatc cgcgattcct cctttataaa aataaaaaga cctgctttca actaaggacg
    15061 aatgactccg cggtaccacc ttaattaaaa acagatctgt ttttcacttt caaacgatta
    15121 ttcaaatgta tggactttcc atgtaattcg tcaattaggc ttgatcggtt cacagcgacc
    15181 accgactttc tgaacaaaac acctaaccac tactgcgtga aaagttgact tgtatgaagt
    15241 ttaccttcca taagatcatt tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa
    15301 ttatttttat aactttcgcg cacaacttta tctttttact agaaagtagc gccctcttgt
    15361 ttttttcttg ctactatttc ttgatgttct tgtttgattt tttctaatag cttgggttct
    15421 ctcaataaat ctagagctgt attggcaagt aattccgctg caatcgcaat agaagcaagt
    15481 cctttttcac ttttagcagc agctttgaat tcttctgtat gtccggcaat atatgtctca
    15541 gagattgcta cagtaggctg tatcgttgga ataacttggc taacatttcc tacatcagaa
    15601 gaacctagac ttgttctttg ttccgtttct atttcttcat caggaacccc tgcttctttt
    15661 gcatgttgga agaataagtc atcgaaagaa ggcgttacga tgacatcatc gactgcattt
    15721 tggaacaatc caaactcaaa agtcgtaccc gtgcttaatg cagcacctcg tacgatattt
    15781 tccacttttt catatacttt gtctaatgtt tgtcgatttc ctgcacgtaa atagaaacgt
    15841 ccagcagcgt agtctggtat gacattggcc gccacgcctc catctgtaat gattccatgg
    15901 atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccgttaaa cacttggatc
    15961 aaggcttcca aagcatttac tcctttttca ggtgctgctg ctgcatgaga agcgacaccg
    16021 taaaatttga tatcgactgg atcatttgct aaagattctg tcgttttccc ataacgaaaa
    16081 gctggatgga cacataatgc tgcatcgaca tcctcaaaaa aaccttcccg aacgaagctt
    16141 cccttagctg atccattttc gcctccttct tcccccggag taccatacac acgaatttct
    16201 ccgccaatct catcaatgaa ttgtttcact atgctggctg ctaatacgga gtaagtacca
    16261 aataaattat gtccgcatgc atgaccaata cctggtaaag catcaaattc tgccaaaaag
    16321 gcaattgtcg gacctggttt attggaaata taacgtgcat caaagcctgt tcggtgccca
    16381 gctacttctt tttctaccca gaacccttct ttttccaatt gtttgattaa agctgctgag
    16441 gaataaaatt cataattact tacttcagga tggtcgtgga tagctagtgc aagtgcttga
    16501 tattctggta aacggtattg aatcagatcc ttcaattgat ttgttgagag tgtggttgtc
    16561 ataaaaattt cctcctatgc tagtcgtttt ttcttatttt ctcccgaatg tttcccacgc
    16621 aggtacgctt gaaccttttg atgttttttc gattactttg gctgtttctt ctgtttgata
    16681 ggcatctact attttttgat acgttttatt gtcttcatct tcttttcttg caacgatgat
    16741 attgacataa ggacgtgcat gctcatcgac tggttctaag aagattgcat cagaagcggg
    16801 attaaatcca gcatctacag ctacaccgct attgatgact gaaatatcta catctgacaa
    16861 agcacgtgct gtttgggaag catctaactc agaaatgtct agattttttt tattctcggt
    16921 aatatcagaa acggtaggcg cttggccttt tgccttgtcc actttgatca atccagcagt
    16981 ttgcaataac agaagagctc tgccgccatt cgtgacatca ttagggattg caacttttgc
    17041 tccatctttg atatcagcca catcttttaa cgtattggaa taaattccca acggtgcatt
    17101 aaccgtattc ccaatcgata ctaaatcagt tccgtgttct tcattgtaat tgtctaaaaa
    17161 gaattgatgt tggaaggaat tcaaatcgat cgatccatct gctaaagccg catttggctg
    17221 attgtaatct gtaaatttca catattccaa atggatgttt tcctttttca aacggtcttt
    17281 cacatcgtcc catacatctg tatcttcccc aatgatccct agttttacgg tcgtttcttt
    17341 tttttcagtt gtagctgctt ttccttggct gcatcctgcc aaccaaataa ttgctacaaa
    17401 tgtaatgact gcaaaacgaa tatttttttt cattttttct cctccatttt caatgtgtga
    17461 cttttttaat cagttgatta ctgataaatt gactcaaaaa aactaagacc agaatcaata
    17521 atgtagcgac aatcgttaca tctgtttgga atcgattata tcctctcgat atagcaagat
    17581 tccctaatcc tccggcacct actgctccag ccatggtagt cagtccgatc acattgatga
    17641 tcgtcaacgc tgagacccga atgataccag gtaacccttc taacaaataa acacgtaaaa
    17701 tgatgccgat tgggctcgtg cccatcgctt ctgctgcttc gatcacccca ggatcaactt
    17761 ctagcagtgc attctcgatt tgacgggcaa aaaacgggac aacgccaaaa atcaaaggga
    17821 ctaaagctgc tttggcacca atcgtcgttc caactaagaa acgagtagtc aaagctaaaa
    17881 gagaaagcaa gataatgaaa gggatggaac gaatgatatt gatcaactta tcaagtattt
    17941 cgaacagcag cctgttagct agtaatccat ttggacgtgt cactactaac gtcacaccta
    18001 agatgattcc gatgatccca ccaatgaatg ccgtccaaaa agacatatat agtgtttgct
    18061 ttgtcgcttc gatgaattca tccggaatca aatagacatt cggcaaatat gttttaaaaa
    18121 attcagtcat tttagtttcc tccttcgatg actttgatac ggttatctaa ttcatttttt
    18181 tgtaaaatag ttacttttac accagattta atcaaataat ccaatgcttt ttgccgctgt
    18241 ttggtttctc cagctaaagt aacgatcaaa cttcctaagg gtgtttcttg taatagctct
    18301 acattaccgt ataagatatt cgctgttact tggtacttgc tgtaaagatg agcgattaga
    18361 ggttcgttcg tctggttgcc gatataactt aattccacca accatttatt tgcaatctga
    18421 tcggcaaaag cagcatgggc actgattgtt tccaatgctt ggtcaaggtg tgtcgctgtc
    18481 cgaataaaat ctttagtcaa ttcttcttca ggatgactga aaatctggat gctgctacct
    18541 ttttcaatga ttttccccgc ttccataacc gctaccttgt tgcagatttc tttgacaact
    18601 tgcatctcat gagtgatcag gacgattgtt agacctagct gacgatttag tttttttaac
    18661 agtgcaagga tctgaagggt cgttttcgga tcaagtgcac tagttgcttc atcgcataac
    18721 aacactttag gttcatttgc cagagctcgt gcgattgcca ctcgttgttt ttgccccccc
    18781 gacagttgtt taggatacgc agatgctttt tcttccagac cgacaagttc cagtagttca
    18841 ttcacttttt gacgacgttc ctgttttgat tttccagaat actttagaga aaaatctacg
    18901 ttgtcaaaaa tcgttcgact ttccattaga ttgaaatgct gaaagatcat accgatggcc
    18961 tttctttctt ttcgcaattc ttttggagag agctggctta aattcgtctg attgacgatg
    19021 acttctcctg ctgttggttt ctgcaacaaa ttcatcacgc ggactaacgt actctttcct
    19081 gcacctgagt aaccgacgat cccataaaca tccccttttt caatcgttaa atctacagaa
    19141 tctactgcct gtatctcttt ttctttttgt ttgaaacata cagaaatatc tcgcaattca
    19201 atcattaaca ttcctccttg tctataaaaa aagcatcctc aagaaaatga aaatagtcat
    19261 tttcttgagg acgccttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
    19321 ctcctcttca agtaccatca tactctagca agatatcggt cgcagccgtc accgccttac
    19381 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
    19441 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
    19501 aatattcaaa aaaaaaatcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
    19561 cgtggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag
    19621 attggcgttc gttttaacgg tcgaacattt ccgaatttgc ctacttatcg acaaattatt
    19681 ctccaaggcc attttcagct tttcttactt gactcttcac accagccaga gcctctctgc
    19741 aaagtttcca agcttacttt ccttctcaac gaaatcaata ttttgttgtt gggaataatc
    19801 tacaggagga aaactgaaaa gtcaagcatg aaatccaaat tattttttct atatttattt
    19861 ttctgttttt ttatctaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
    19921 gttcaatttt tccttctctt cgacgctata atttcgaaca aaactggaat cgggaatcaa
    19981 aacaacgcct agtcctgcac gtgaagcggc tgtcgctcct actaaagaat cttccactgc
    20041 aaaagctgaa agagcttcta atcctgttcg ttccaaagct gtcagataag ggactggtga
    20101 aggcttgttc tccgatactt catctccgaa cgtaaacgta tcaaagtaat cggctaattg
    20161 aaagtgatcc aagatatctg ttgctctttt cgtaacagtt gtcgtagcaa tccctagttt
    20221 catatgacgt tcttttaagt ctgtcaatat ttcttttgca tagggtttca attggatagc
    20281 tccacttgtc agctgttgat aaaaatattc ttcacgcttt gctcggactt cctgcacttt
    20341 ttcaggactg cctgctgcct gcactaaaat atcaaaactt tgtttccagt tttttccgcc
    20401 ccaagcgtca acgatttctt tcgggatatg aatatcaaat tgctggaatc cccaaagcca
    20461 accatcccga tacgttcttt ccgtatccac taacaaacca tctaaatcaa atattgcacc
    20521 tttcatctat acattaatcc tttccaaaac aatacaccta gtttagcatg actgatccaa
    20581 aatgaacaga agcatagagc ccagccgcat cacttataag agttcttgga atcgacggat
    20641 atagattttc ttcacagctg tcacaagcgc cagataagcg acgactgtta taaacaacca
    20701 taaccagtaa ttgccaggga gcggcatcaa gcctaactca gcaccaaatc ctgtgaatgg
    20761 aaggactgaa ccgacaatga ttcctaatgt agtgataacc agcatggcaa atgttgcatt
    20821 gctttggata aatggtattt tcggtgtacg caatgcatgg agtactaatg tttgtgtcca
    20881 cagggattcg acaaaccatc ctgcatggaa taatgcgata aaaatcactt gttgtgagct
    20941 atccaatgta tggaaacttc caccaactac agcaggacaa ataacaaaat acatcaacaa
    21001 gtaagtagtg atatcaaaaa tcgaacttgt tggaccaaaa taaaccatga attttccaat
    21061 actagatgca ttccatttct ttggctcatc caaatattct ttatccatcc gatcccatgg
    21121 taaggaaatg caagaaacgt catagatcag attcaaaaat agaagttgca aaggcaacat
    21181 tggtaaaaat ggtaagaacg tactggcaat cagtacggaa aacatattgc caaaattaga
    21241 acttgctgtt gccttgatat atttcatgat gtttccaaaa gtttctcttc cagaaagaag
    21301 gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgctg attcttttgc
    21361 gatatccaca gctgtatcca cggaaatacc gatatcagcc actttcattg ctggagcatc
    21421 attgatacca tctcctaaaa atccgacagt atgcccattt ttacgtaaag ctgtcgtcaa
    21481 tcgtgctttt tgctgaggat tcaattttac gaatacttcg tgttcttcag ctacttgcgc
    21541 taattcatga tcattcattt ccgagatttt ctcaccagta atcaattcat tgatttctag
    21601 gcctacttga cggcacacag accgtgtgac taattcattg tccccagtca gaacttttac
    21661 agctacacca tgttctttta aagctttcaa cgcttgtttt gttgtttctt ttggtggatc
    21721 aaggaaagca agataaccaa tcagaaccat gtctgactca tctttgactg aaaattctcc
    21781 aacaacgctt ggattggtct tttgtgcgac accaatcact cgtaagccgt cttcattaag
    21841 tgattgtact tttgcaagca ccgcttcttt tcgttctttt gttaatggtg cgacagtacc
    21901 accaagatca ataaatttag aaatgttcaa catttcttca atcgcgcctt ttgtgatcat
    21961 ctgtgttttg ccagccggat cagcgactac aacactcata cggcgacgtt caaaatcaaa
    22021 aggaatttca tctacttttt gataattgat tgtatctgta tctaaagttt ctgctgcttc
    22081 atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc tgtttagata
    22141 agcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat
    22201 gattttgtct tgtgtcaatg ttcccgtttt atccgtacat aacacatcga tcgcaccaaa
    22261 attctggata gcatttaaat ttttgatgac tgttcctttt ttggccatca cacttgctcc
    22321 tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggcgttaaac caactgccac
    22381 agataatcca aaaagaaaag cttctaacca atctcctttt gtaagcccat tgataacgat
    22441 aactgctgga gccatcaatg acataaattt gatcagcagc atggatgttt tgtggatgcc
    22501 taattcaaaa cttgattgga tgggtttttc agatagcgtt tctgctacat gcccaaatag
    22561 tgtgtcattt ccgacagaaa caatgattcc ttccgcgctt ccactgatga cattgcttcc
    22621 cataaagaca agattttcat aacttgtttc actttgctga tcgtcttcac atactttcgc
    22681 atgcttttct actggatagc tttcccctgt caatgctgct tgagaaatga atagatcctt
    22741 tgcggacaat aaacgcatat ccgctggtat catatctcct gctgaaagcc gaacaagatc
    22801 tccacagacg atttcttcca taggaatctc tgtatactcg ccatttcgtt tgacagctgc
    22861 cgttactttc acaagtgatt tcagtttttc agctgcttga ttagaacgca cagactggat
    22921 caacgtcatc gtcccactga cgataaccat agtgaagata atggctacac caaagagatc
    22981 tttatctcct ggagcggcta atacgtattc cgtaatgaat gaaataattc ctaatgcaat
    23041 caacactaat gtaaagggtg tgatgtatgc tttcaatatt tctataatga atggtgtttt
    23101 tttgccatat gaaatcgcat tttcaccata agtttctctt gaacgctcga tttgttcttc
    23161 tgacaatcca tttggtgatg catggaacaa ttcaaataat tcttgcttcg tttttccagc
    23221 aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcgatct ctcctttgtg
    23281 tccaaaagac agctgttctc gctgtttttg gtcttttgct attcatcgga tcgatagaga
    23341 aaatgctgaa aggggctgag aacagataga taatagccct ggcctttcct ctaaatcctt
    23401 gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca
    23461 tgacttccaa tcatttttcg aatagttatt ttcccaactc gctcacctcc ctttttgttt
    23521 tttcacctat gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt
    23581 tgaaaaagca aatcaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa
    23641 cgtaaaaagc agatatctgc ttttagctac cttatgaaaa gccttatctc gacaattcct
    23701 gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc
    23761 tcacctaaca aatcctattc ggttttccct cgttaatata acaactaaaa aagaggatgt
    23821 caaatcaatt ttccatgttt ttcatacttt tttaaaatac acgcgttata tttcctgtct
    23881 cacgcagttt tatttgtctc attttccacc tcccgctata atagaattat agtaggaaac
    23941 gaggtgctgt tatggaacga acacttgtta ttatcaagcc agacggcgta agacgccatt
    24001 tggtcggaag catcatccaa cgttttgaag caaagggact ggccattgct gaaatgaaat
    24061 ttgcaacgat gacacctgaa ctagcaaaag aacattatca gcacttgact gaacgtagtt
    24121 tttttgatga gcttatcgat tacatgactt ctggtccggt cgtttatctt gtgctggtcg
    24181 gcgaggaagt gatcgatatc gtccgtaaga tggtcggagc aacaaaaggg gcggatgccg
    24241 ttccaggtac gatccgagga gattatgcac tgccggggac tgaaaacatc atccatgctt
    24301 ctgattcccg tgatgctgca acaaaagaga tcgcccgctt tttcccgcca ttcgatacaa
    24361 acaaaccgtc tgaaaaagcg gcgaagtgat acaaatccta aactgttggt ctaactgctg
    24421 ggtttagcaa tggccagcag ttttttctct aaagaattcc tttgattctt ccctaatgta
    24481 tattcgatca ctgcgaaaaa ctcttggtga ttttccgatt tttcttcccc taaagcagca
    24541 gaaatcagtc gttggtaata atccgccatc aatgtttcat aaatcactgt acttctttct
    24601 ttatgcgcta aactccgttt catatcttca ttcacgctac gtgccttttt taagcgacca
    24661 ttttctatat aaaacaaagt caagttcgtc aaaaaagtaa agagatactg ctcgtgatta
    24721 ttcagttgcg cgatccgtcg cattcttttt ttgattttca aataattatt ctcaataaaa
    24781 cgatcactga atatccaagg aaaatctgct agaatagata cttcaaattg cccatactct
    24841 ggtacttgca tcaaatattc ttcaatgata cgacattcat aacgcaaaaa ttcaaattcc
    24901 gtctcagata cttctgtttt tttggacatg tgcagattca aaaccaaata gtacagatct
    24961 tctgttttct tatagtgcct gcggttatta gcaatttctt tgtaaggatc aaagctgtct
    25021 tcttgaatcc gatctagcat tcgtcttttt ttctttaatt tttttggctc atcaagctga
    25081 tttcgtatcc attcatattc ggcaagtgtc atgttcattc gctctaaaaa tgatttcaat
    25141 gttccaaacg atactttttt cactgaattt gtctcgatcc cgactagcga agttttagaa
    25201 cagattcctt ccgctaattg ggtctgtgtc agctttttgt tgactcgaag cttcttaatc
    25261 aattctccat catacataat agttctcctt ttttaattga aattaaccct tggcttttcc
    25321 agaatgcttg ccaataagag gaatgattat ttcaatttaa attttccgca ttcaataaat
    25381 agatacgtta aaaataaaaa atacacgaaa aaaataaaaa aatgtttttc tcataaaaaa
    25441 taccaacctt caagcatgag actgctgtct aatccctgaa ggttggtttg attgtttatt
    25501 gatcattttt ttactgctcc gcattggttc ctgttactgg atataggata gtcgttgttt
    25561 tattgccgtt ttttgaaaag acactggtcg aatgttcacc tttttgcttt tcgattgctt
    25621 cattacgttc cgcttcgttt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt
    25681 ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag
    25741 tcttgacttt ttcctgatct ttggagattt cctcgtcgat tcctgctgct ttcgtatcga
    25801 tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggcgtca
    25861 cgaagttccc atttcgaaaa gcaactactt gatcgtgctg agaagataat aagtctgtac
    25921 caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat
    25981 cgatttctcc accatattca tgattgactg tccctttagt gtaaccaggc atatggatca
    26041 tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac
    26101 caagagcact tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa
    26161 tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca
    26221 atgattgatc cagataatgg gccgttcgaa cataattatc tactgtgcta ttgccagtat
    26281 tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg
    26341 aaagaaattt cgtgtaaaac ggctgttgta gatgttcaag atattgggca gattctctga
    26401 ataaatcttt atctaatatc ccataaccta acgtttcatc ggattcatca aaataagatc
    26461 gatcaaagaa attgtcatac cctaaatttt tatatacatg atctctattc cagaaactag
    26521 ctacattccc atggaaaaca gcacttgtat agccttctgt ttgaccaagg gtcgctggtg
    26581 ctgcttgaaa gacattatct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa
    26641 atgtgccagt ttccaacatg ttttcagcat cacttgtctt tccctgtcct acttcatgaa
    26701 agaaattgtc aaaactgatc gttgcttgat tctggaaaat actatttaaa aacggcgtga
    26761 cttcttgtcc gtctaccttc atattgatca aaaactgttg aaaactttct aaatgaagga
    26821 caatcacatt ttttccttta gcaatcccaa atgtttcagg atttggctct gcataatgtt
    26881 tctttgtata gtctaaaact tcatcaattc cattactgct tgcatgcgcg cgaacactgc
    26941 tagtcattcc tgttttgatt ccgtcataaa tagtgtacgc atcgattcct agatatttga
    27001 caatatagga acggtcaaat gtccgctgta gcaattgggg acggttggct tcgctcaacg
    27061 ctaaatttcc tgaaaaaatc aatcctgaaa gacaggtaac agcgatagcc atcggtttgc
    27121 tgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga
    27181 acacagcgat atccaaccaa tagatgacat catggggttt catcaaagaa aaggagcttc
    27241 ctgacaaccc ttgtgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg
    27301 tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga
    27361 tcaaaagaat ggctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca
    27421 ataaggtagt cgcaatcggg ttgatccaca agataaagta ctggatcgta ccttccacac
    27481 ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg
    27541 tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt
    27601 ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt
    27661 gttatcttaa gattttttta ttctttctaa agattattcc attcattttt cacagcataa
    27721 aatcgtttcg gtgtaagcgg ttaaaatagt aaggagataa aaatcttgtg gtgaccgatt
    27781 tactctatag tattcaaaag tagtttctcg gtattcgtac aaaaaaaaca tactaagatt
    27841 agaggggaac atcattgacg aatgatgttc tcctctattt tctattttta gattatagtg
    27901 aagagtgaaa gaaagttcga acaaagcctt gaggactacg agtccctatc aaaaaccgaa
    27961 cttctttact gtcattttag aatcaggttt acgtatgttg gggccatgag cccgtgtata
    28021 ggaaattgga atcgaaatga agcagtaaag ttctttcaac acttaaaaag gaggaaaaca
    28081 aatgccttac attcttgcat tagatgtttc aatgggacac agctacagtg tcctatacaa
    28141 agacgacacc tgcttatttg aagacgagat cgaacacact caaagaggat ttcatgcatt
    28201 actcgaagag atccagcact tgcctgaacg tccattgatt gtttttgaag caacgggtat
    28261 ttattccaga ccagtcgaaa agttctttca agatcatcac ttttcttact gtttgttaaa
    28321 cccgttagaa gcgaaaaaac agatggaaga aaacacctta cgcagttgga aaacagataa
    28381 atcggatgcc catcggctcg cacagactca tcttaaaaat caacgtcaac caaaagaagt
    28441 acaaagcaat ttttacttag agatgcgcga tttagcccgt ttttatcaag aaattgagaa
    28501 gaaaatcact cgactgcgca tggatttaca caactgcctt cagttgactt ttccagagct
    28561 tgaacagttc ttttcaaaca ggctgacccc ttacgcattg acattgatcc gtctttttcc
    28621 ccatccagat tttgtattgg catctactcg gacaaaaata aaaaataaac tgatcaacga
    28681 aacacgggaa aaaatctccg cgaatcgtgc cgagcaaaag gcagatcaaa tcatccacta
    28741 tgcacagtgt gcctatccag ctgttgaaaa agacagtatt cactgtcaaa aaacgatcta
    28801 ttacgccgaa cttctccaag atttactcga acaaaaaaaa gcactggcga cacaaatgat
    28861 caaaaaagcc gaagcctctc cttgcttttt gttgtaccaa acgttccctg ggattggcgc
    28921 actcacagcc gctttattgc tcggtgaatt aggagatatc acacgattta aaacccataa
    28981 acaattgaat gcctttgtcg gaatcgacat ccgacggtat cattcaggta agtatactgg
    29041 ccaagaccgc atcaataagc gtggtaatcc caaagcacgg aaaattattt tcttcaccat
    29101 tcgaaatatg attcgccagc aacgcgcagc tcctaatcat attgtcgatt attactacaa
    29161 attaaaaaag caacctatcc ctaagaaaga taaggttgcc acagttgctt gtatgaacaa
    29221 gcttctgaaa tgtatgcacg ctatggttag ggcacataca gagtacgact atgcgtacgc
    29281 ggtctcagtg gaccattaac taaaccactt acagtatacc acttctttgc ccagatcgaa
    29341 aattttttac gaacggggct ttatttagtg tgccttgatt ttgtcgcttt cgtttacgag
    29401 ctatttttct tgcacctttt acttgactaa tcgtaggaaa gaggctgtga caaaattctt
    29461 gtcgcagctc cctatttctt gatgcaggac agctttttca caacctctcc actaaacggt
    29521 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt
    29581 cccgtttttt tctctaatac tcaggtcaaa ccgctgattt cacaacctct gattcacggt
    29641 gttccagaag tagcttcttc ggaaataagc cgaaatttta caaaaacttg agaagcactt
    29701 ttcgtaaatt tcttcttatt tcttgaagct gaccacttct gtcacaactt ctgtttttca
    29761 ttgtccatag gtttgatatt gtccttctga tgactcattc gtaatgattg aggcttgatt
    29821 ttcttcattt cccgttatcg tttgtccact gccagaatct gaagggctta gttctgtcga
    29881 ctcgtttttt tgagaagaag cagttgtttt cttgacttgt tcgttggtct ttttccattc
    29941 cgctagtcga tcggccacca cttttttgtc cactttttca gaataagctg taaccacttt
    30001 tccatttttc aaacgtgcta ccttgtcgcc atcatgagtc aaaagcaaaa cgaccaaatg
    30061 tttgtctttg aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattgat
    30121 aaattgattt tcttcttttc cttttggcaa atagatgtcc aattcacctt tttcgtaatt
    30181 ggcagttaat ttatattcct cccaccaata agttgagaat tcttttttga aattatctac
    30241 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    30301 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    30361 aaacggtctt tttttcctca tgccttcctc cactcctatt taattttgac tattctatta
    30421 tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt
    30481 ctttttcctg aagcggtaag aacgggaatt ttcatacatt ctctcttggc tattctttaa
    30541 gcttcaaatc catttctgtc tgatacatct ttgaaccaat gacccgattt cttgatcgtt
    30601 ctttttccat cttgtgctaa atctactgaa ataaaaccgt aacgattctt ataggcattg
    30661 gtccacgacc agttatccat gcatgtccac atatgataac ctaaacaatt ggcaccttct
    30721 tgaatggctt gatggacata tttcagatga gattgaacaa attcgatccg ataatcatcg
    30781 tggatcatac catcttcctt gataaatcgt gcttcatttt ctacacccat gccgttttca
    30841 gaaataaaac agtcgatatt cccataatga tctcgtacat tgatcaaaat atcatagatt
    30901 cctttttcat agatctccca tccgcgataa ggattcattt tacgaccagg catttcataa
    30961 ttgtcgaagt aatcctcagg cataggaccg tttttagtat cgattggtgt ttcttttgct
    31021 tttattcggc gtggttggta ataattaatt cccaaaagat ctactgtgtt ttctttaatg
    31081 atcgtcaaat catctggatg aatctccggc attagatcga gttcttttat tctctcaatc
    31141 aattcactgg gaaactcgcc ttttacagct ggatcaagga atgaacgatt gaagaacgca
    31201 tctgctattc gtgctgcttt aacatcttcg ggatttgatt cgtctcttgg ataactcggt
    31261 gtcaaattta agatgattcc gatcacaccg ctttgatcca tttcatggta agctttgatg
    31321 gctaatgctg aagccaacgc ttcatgataa ccaacttgga ttgccttttt catatctgat
    31381 tcttccggat aatgccatcg atacaaatag cctgcctcga ctggaacgat tggttcatta
    31441 tgggtaaacc atttttttac tttcgagcca aacaattgaa aacaactttt tgcgtatttt
    31501 tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag atacatcggc
    31561 atatcgaaat gatacagatt aacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa
    31621 tagtgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc aggtatcaaa
    31681 cggctccatt ggatagaggt tcgaaatgaa ttatgcccag tctgtttcat caactgtaca
    31741 tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg atcaaagaac
    31801 ttttgcggtt cgtttttgta ccaataatca aaaatattct ctccttttcc atcgcctgaa
    31861 attcttcctt ctgtttgtgg accacttgct gcagagcccc accaaaaatt ttttggaaat
    31921 tgatatcttt tcatcaaagc acctcttttt cttttttagt tgattccatg tatgatattt
    31981 tccatcacaa tcactttttc attttcttcc tcttcttttt tgattcgttt caacagctgt
    32041 tctgctgctc gttccccgat tttatctgtt ggtggcgtga tcgtgatttt tggaaggata
    32101 ctcagatcgt ttttatctaa ataatcatag tgactgaacg caatatcctt cccgattctg
    32161 acaccttctt gattgaaaca ttcgatggca ccaatcgaca tgttgtaatt gctgacaaaa
    32221 atagcagttg cccctttttc taataatgct tttgccccgt aatagccgct ttttctagaa
    32281 tagtctcctg taaagatctc taaatggata gacggatcct tttgtaccgc ttctttggct
    32341 ccagctaaac gttctcttgc tacgtaatct gtttgagcgg cagcaataaa tcctattgtt
    32401 ttatgtcctt gtccgccatg atgagcaatc acttttgcta cactttctcg atcattcact
    32461 actattgtat caatacctgc tggcccaccc ggacaattca atgcgacaac tgggatactc
    32521 atttttgtca gcatttcaaa tccagaccac tcttcttctg ataagaagac gatcaatcca
    32581 tcgatcgcat gcttcatcag gtattcgatt ttttgttcga ttttttctgg ctgatcggca
    32641 aacccgctta ataataagct atacccattt tcttcagcta ctctttcgat ccctgaaaca
    32701 acctccgaac caaatcggtt agatatcccg tttatcaaca gtccaataga gaaacttcga
    32761 ttattcttca atcctttagc aatgatgttt tctttatagt ccaacgctga aatcgcttct
    32821 gatatttttt ccatattctt ttccttcagc tgctgaccgt ttaaatagcg ggaaactgtt
    32881 cccactgaaa caccagccat cttagccacg tcacggatcg atgccataca ttttcactcc
    32941 tgccagttac atttgatttc tttttcttgt tgttcgacca tgatgaccgc tttctttgca
    33001 taatgccgct tgtcatcaat atccatataa ctaaagtctt cagaagtgag ttcaaaaaca
    33061 agcagtcttt cctcttgaga ccgcaattca tctttacgaa aagctttcaa taatttcgtt
    33121 ctttggatga caacgccgtc aattaatcgg acatagacaa gagtacttgt tttacctgaa
    33181 acacttcctg tatttttaac tttaattgat attattactt tgtcaatatc cgttttgact
    33241 tccatctgac tgtattcaaa tgttgtatag cttagaccat gtccaaaagg ataaaatgga
    33301 cttccgctta aatcataata attctcttgt ttagaagcag cacgctgata gtaataaaca
    33361 ggtagttgcc gactatttct aggataagac aaactcaatc gtccgcttgg gttatttctt
    33421 ccagaaatca tctgcgcaat ggcttttcca ccctcctgtc caggaaacca tgctgttgca
    33481 atggcattcg taaatgactc gattttcatc aaatcgtagg gtcttccttg cacaagtatc
    33541 gtgatgattg gcttgcctag ttgatacaat tgttcaatca gctgttcttg gtatccacct
    33601 aaagaaagag aagcaacatc tacattttct ccagaatcca tgttgatccc tttcgatgaa
    33661 actgccccgt tacgaagaaa ttccatatca aagttccgcg cacttgatcc acctagaact
    33721 gcaatgatca catctgagtt ttgagcaagg gtcaccgctt ctaataattt ttcttcctga
    33781 tcctcctgat ttcttacttc acatccttgt gcataagaaa cctttgattg gggaaactct
    33841 gtttttatct cttgtactat cttttttttc aaacggtctt ctgtttgtgg tgcagagtag
    33901 tctcccaaca agtggtaaac atcgtgagca tttggaccta taacagcaat gttttttact
    33961 tgttgatcca acggcaatat accgtcattt tttagtaaag tgatactttc ttttgctgtc
    34021 tctaaattta agtgctctga ttcctccatg agatgatccc aatactcctt tggatcgttt
    34081 gcaaatggct gttcaaataa acctaataaa aatttgacag ataaaactcg gcgaactgct
    34141 tgatctagta gcttttcatc tacgacttga ttatcaatgg cttctgcaat cttggtatag
    34201 gtctcatccc acaaactgag atcgattcct gccgctaatg catatgctgc agcagtcttt
    34261 ttatctgtaa aaacatcgga taaacgatcc aaagccacac cgtctgccat aacgattcct
    34321 tgataaccga tctcttttct taatgtttgc tcaaaaagcg ctcgattgat atgacaaggt
    34381 atcccgtcga tgtcattata agcagccatg acacctactg catttcttgt actcttcaat
    34441 aatggtccgt aaatatcgga gaactctctg cgtccaatcg ttactgtgcc tgaattatga
    34501 ccgcctaaag cttctccttg tgcgatacag tgctttaaca caacaccgat ctgatccttc
    34561 ttcttattga tttcctctac tgtctgatca agaaaagaaa ctttttctga aataatctct
    34621 ccttggaacc ctcgaacgac cgcttcagaa aaacgagctg agagaatcgg atcttcccca
    34681 aagcactctt ctgtccttcc ccaacgagga tcttttgcca gatccaaagt cgacaccagc
    34741 gctaaatgaa cacctttagc agccagttct tttgccatca atcgactcga ttgttctaat
    34801 aatgctacat tgaaagtatt tccacggccg atattcgttg gataagaaat cccatccaag
    34861 ccttgatgac catgtggaca ttcttctaca agcaaaactg gaattttcca tcgcgaatgc
    34921 tgtagtacat attcttggac taggttagcc accttccagc tttcttttgc aggaatccca
    34981 ttcttataat ctgttttaga ccacggatca gagcgaaaca gaccgtataa ggcacccaga
    35041 ccgcctcccc atttaacatg ttcttttaac ttttctgtta attcgatctt gtctccgttt
    35101 ttttcataac attcccaacc atacaaatgc tggtttacct gtcctacttt ttcagcaata
    35161 ttcatcctcg aaagtaaatc ttctactctt tggcggatgg ttaactttgg gttttgatac
    35221 gggtaatcgt acaccttaat atcctccttc tgccaatttt gccttattag cggctaatac
    35281 aaatggcagg aataaaaata catctagaat gataagtatt actgttaaga ctgctgctcg
    35341 tatatcgcct cctgtagcaa taaaagattg tataaccggt ggagtgatcc aaggtgcagc
    35401 agcaactgtt ttactgatga ttccaagact tgtggctgcg taggcaataa ccaagttaat
    35461 agatggtata atagcgcatg gaatcatata tattggattc atgactactg gtaatccaaa
    35521 cataattggt tccgtaatat taaataaaca agtagtcaga ccaaatttag cgattgatcg
    35581 ctgttcttga cgtttagaag cccacaagat agcaataact aacggtaaaa tacacccgcc
    35641 acctccgatc aatccaaatg cattggtaaa gggctccgta ataatattag gaatttcttt
    35701 tcctactgaa aaagcatcca tgttttcctg aatagactgt aacaaaggtg cttgccgtat
    35761 aggagccaaa atactcgaac catgcatacc aaacacccat aagaaatcag agaaaaactg
    35821 taaaaataag atgcctggga cggataaaac aaaagctttc aatggtgtct gaatcgctaa
    35881 ttcaattaat tgcggaacat tcaaaccagt cgtatggttt aaagtaaaaa cgacaactga
    35941 aaaaaataaa atgacaacta tttctggtac taaagaatta aatgacgcag ctactgccgg
    36001 tggaactcct tctggcatcc tgattttaaa actcttcatt ttcgctaatt tgataaataa
    36061 ttctgtactg acaagggcag caatcatcgc cgttgctaag ccagacgaag aagtcagact
    36121 ttgtaagaaa acccctgtag cttctgctgt atttccgtcc atcaaagtga ctgtacttga
    36181 taaagacatc aaaataaaca ttacggctgt acttagaatt cctgcatgaa caggactaaa
    36241 tgatggatca tcaatcacgc ctgtttggat atagtgggtt gccaaattcc agccgatatt
    36301 gtaacacacg ataatagcta aaatactcat cgtaccatta ttcacgattg aagcaagttc
    36361 agccaatgga cttagatcaa gtattctttg gactgttgca tttgaaaaaa tcagagcatt
    36421 cagcaaaacc atgacagaag cagccattac taacggcata gtaacgataa aactgtctcg
    36481 aatggccagc aagtgtcgct ggctactcat cttagatacg attggtagca ttttttcaga
    36541 tagtctgtct acgactttca ttatttcatc tcctagattc taaaattgaa ccggttcatt
    36601 tttaatatag cacttttttt aaagattgca acacattttt ttaaaacgct ttctaaaggt
    36661 ggtggatttt ttatgacttt tgaaaaatta ttctatttaa tggtattttg tgaatttttt
    36721 ctcttccatc cgtttgaagt tttactttcc aatggtataa tttctttatc ctatcaaatg
    36781 ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat
    36841 tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt
    36901 ctgtcttaaa agcggaaaaa gcagatatca ccaacagtgt atatacgcta aaagtacgta
    36961 aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac
    37021 gcacgtatca gacacaagga gctgattctt ctgaatcatc tgaagaaaaa acagtgctga
    37081 cagatatcaa gattactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa
    37141 aagaagcaac agtcacattt accaaaatcg gtaattaccg tttgaaggat aaggatggca
    37201 acatctacag tctatagtgc catctgttgg atcttctttt gtttctcccc ttcttttctg
    37261 ctatagtaaa gaagcaagat gactgtaaag gagacgaaat ggatgatcga tagtcctgat
    37321 tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgatca aggaagtttg
    37381 agtatatgcg acaattgcca caaactagtc aaagattctt ttaaatcgtt tgaatcttat
    37441 cctgtcaaaa attatgtaaa aagcaaaaga agataagcaa aatcaagcaa aactcactat
    37501 ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc aaaaactgga
    37561 atcagtaaaa caaacagtgg atcatttcag ttcacaaatc aaagagtaac gttttattta
    37621 tttcacatct ttgaatataa aataaccaaa cgatagaaag acttattcta catcacgtag
    37681 taatttttct ttcccgtcgt ttggtttttt acttttagcc taattcttga cctttcgcat
    37741 gctgattatc tgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt
    37801 ggatctgacg aatcaccttt tttgcatctt ctggattttc tacaaaatcg aatgtatccc
    37861 catccacttg tattttagga catacatcaa aatcctcata ccattgatta tagcgggtgt
    37921 tcagtgtttc gtaataggtg tataacgttt catcgctttc tagctgttca tagtcacgcc
    37981 cgcgtttttt gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat
    38041 caggacgttt ttttggagca acatcatcta actcgtttag catcgtatct aataagctat
    38101 cgtactgctg tacttcgata tccgttaccc gacctaaatc agcatttaga tggaacaaca
    38161 gactatcttc gtagatggag cggtctagca cattgtcatc gttcactaaa gcatttttca
    38221 ttgctaaaaa tcgcttattc aaaaagaaga tttgcaataa aaatgcgtac tgctcaggat
    38281 ttgaataaaa aagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat
    38341 caatctcttt tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa
    38401 tcaaaatcac tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa
    38461 caggcaattt ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtgtgaa
    38521 gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc ttgtacgatg atcatcggtt
    38581 ttttcaacat agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct
    38641 caaagtcaag gatagccaaa acaaattctg catttgtcaa ctcttccata tctttttcgt
    38701 atacttcctg cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg
    38761 gagagtagaa acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc
    38821 gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc
    38881 ctccttcaat aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa
    38941 tggattgttg gaaaagatca cttgatccaa ccatttttta tccataaaat cctcttttgc
    39001 aaaagtttct aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat
    39061 tggcttatca ggtgtcaggc cgatcgctat ccaagaaaga agttgcgaaa attcctccaa
    39121 tgtacctaga cctccaggga aaacgaggac agcatctcct ttttcaaata ataatacctt
    39181 tctttcatca attgtctctg taggaataaa tgtagtaaca tctttcccag gcagctcagt
    39241 ttcaaaaagg ccatttggat agatacctgt gacttttccg ttattttcca gtactgtttt
    39301 actaatcact cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg
    39361 tgccatatat tttcctaacg tttgcgcaat aaatttgtaa ctttctttat tgccaaaacg
    39421 ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt
    39481 cattctacac gtgttttttt aaaataaaag actagatttt gtagtttttt agtggtatat
    39541 agatcaaaca ccactatata tagtatttgc aaaaaaataa tttttcagct attttacagt
    39601 aaccaaaaaa ataaataact agtaaatata aaattgaaac gacttactta tagaagaaat
    39661 tgggatttcg tcctgcatgt caataacacg cattatttaa atagtcacac acttgtttct
    39721 cctattaatg aaaaatccat tttattctcg tgttatgttt caccaacgcc tgatgtttct
    39781 ctaaataaaa aagccgacga accaaataat cgaaatttga tcattttggt tcatcagctt
    39841 gttcccaaac ctttgtatta acgataaata gctggattcg taaaactatc acttggatca
    39901 tcactgctgg aagaaaattc ggtgataacc gcgcctgttg cacctgcctg gaaccagtgt
    39961 ttaatatttt gttcgatcgt gtactgatct ccagcgtgaa gtatgatttc tttttttgcc
    40021 gtatagtaac gttcttgccc ctttggaata atacaggaaa gtttttttgt ttcttttcct
    40081 tcgacataaa gatatactaa gccttttcga caacgaaagg tttccttttt tccttcattc
    40141 tcatctcgag gtgggtgccg atgctctgga caagtttgat aaggcaataa taccagttct
    40201 ttcccacagt atcgctcatt attttcataa acaaataaat tcaatccttc agtctcgatt
    40261 gctcctaatc caaaatctgt atattcgatt ctttctaatt ctgtttcttt caatactact
    40321 cctgaatcta ggaacatttg ttgtactctt ttcttgtaat ccattctgtt cctcctaact
    40381 gattgtcaag tcatcccaag atatctcttc ttcctctttt tcttcttgga tgacttctgc
    40441 atttgggtca actggttttt tcaataataa aatagttatt cccgtagcaa aagaaccagc
    40501 taacaatgca ccaatgaatg cccatggtct ggtcatcgta gcgactgcaa acattcccga
    40561 tgccggaata cggctatctg cgccccaaaa catgctcaat ccaccgccga ctgccccacc
    40621 gactcccgtt gctatgacat tgcgtgccaa gtcgttcaat acgataggga aacatccctc
    40681 tgtgatctga cagatcccca taggaaaagc ggtcttcaat gtatcgattt cttgacgatt
    40741 gaatatattt ttacgaatga ttttccctaa gaaataagcg atcgtcaagc cgaatggggt
    40801 taccatcgaa gctaaaatca atacagtgat tggttcatag attccttctg aaaatcgtgc
    40861 cacaccccct tatccagaat aaatggtaat ccagaaacag ccgggtatct gcggtaataa
    40921 gcctaaccaa acgaaaaata tgagaagcta atatgagaag ctatttttgt ttggctgttc
    40981 ttattacttg atgcagggca gctttttcac aacctctttt tttatttatg tttcataatc
    41041 aatatggtaa ggatgcagct ccgtatggta gaccgcctcc gaatattcaa tcatttcccc
    41101 aatttgcgta gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat
    41161 ttcttgttct tcttctgtta aaaatactgc cgagaaatca tcagaaaatg agtcaatagt
    41221 atatcctttt ttcgtcagca aacgatacaa agattctttg ccaaattctt ctctgctgaa
    41281 ttgcatcatt tcttgtggaa gataatgaac aaaataaata taaggctgat tgttcaaata
    41341 atataagcgc ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag gatcttcctt
    41401 ctttatatgg accaattgca aatccagcgt ttctttccgg acttcatatc gggagttctc
    41461 caagatttgc gtaaagcttg ccgctttgga taatcgattg taaggacgat tgcttagaac
    41521 cgtcgtccca cgaccacttt tctttgcgac atattcatct gatgccagca gttcgattgc
    41581 ctttcggaca gtgattttgc tgacttgaaa cagttcttct aattcacttt ctgtcggtaa
    41641 catcgaaccg actggatatt ttcctgagaa aatatcttct ttaattttat ctgcgacttc
    41701 caagtatagt acacgtgtcc ttttcataac gtcacccttt gctttactat tactaaaaga
    41761 atagcacata aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg ttctttttca
    41821 ttcagctgct ttaattacaa gtttacgcca tttgaggcaa ttacttcttt ataccaatca
    41881 aaagattttt tctttgatcg attcagtgtc ccatttcctt catcgtctag atccacatag
    41941 ataaagccgt aacgtttact catttctcct gttgatgcac tgacaagatc catacatccc
    42001 caaggcgtat agcccatcaa atctacaccg tctgctactg cattagccat cgcttcgata
    42061 tgacgacgta aataatcgat ccgataatca tcaataatcg tgccatcctc ttccatatga
    42121 tcaatggctc caagaccgtt ttcaacaatg aataatggtt tctggtaacg attgtataat
    42181 tcattcaatg cgattctcag cccttctgga tcgatctgcc agccccattc acttgcctca
    42241 agaaacggat tttttactcc tcccagcaag ttcccttctg aagttgctgc ttgtggatca
    42301 gtctcattga ttgccgtaga catataataa ctaaatccga tgtagtccac tggatattct
    42361 tttagcaact ggaagtcttt ttccgtttgc tctaattttt ccggatcgac tccatatttt
    42421 ttatgggtac gcgctgtgta tgcagggtat tctccacgta cttggacatc cgtgcagaaa
    42481 aaattgaatt cttggttttg gatcattgtt gctgcctgat tgactggatt ggcatcgatg
    42541 ctgtaagttg ttgcatagat aatcatgcag ccgatttgga tatccgaacg caattcgtgg
    42601 ccgattttta cggctttgct acttgcaacg aattgattat gccatgcttg gaaaatattt
    42661 tggtactctc ctgctccatt gctttttact aatccttgac ttaaagctgg aaagtgaaag
    42721 gccgaattga tttcattgaa agtcatccaa tatttgactt tactgctgta tctctctaat
    42781 actgtctgag caaatcgttc atagaagtcg atcagttttc gatttttcca gccaccgtat
    42841 tcttttgcca aatgaagagg catttcgtaa tgagaaatcg tgatcacagg ttcaatatca
    42901 tattttaagc actcatcaat cagctgatca taaaattcta atcccgcttc gttaggagta
    42961 ccttcgtctc catttggaaa aattcgtgtc caggcaatcg aaaaacgata acatttgaat
    43021 cccatttccg caaacaaggc aagatcttct ttgaaacgat cgtaatgatc gattcctcga
    43081 tgattcgggt aaatatattt ttcttgatca atcgtccagt caaattcttc actgccgata
    43141 atcgcaaagc gctgcttacc tccaggcata gcatctgcaa cggatagtcc ttttccgtct
    43201 cgatcataag ccccttctaa ttgatttggc agcagttgct ccaccccata aaaatttttc
    43261 tgaaaaacgt gattgcatcc tgatttcctc cttattttac aattgccaga acatcttcgt
    43321 ttgcaataat ctctttttgg tctaattcaa gtatatcggc aaatgcatca gtattcgtaa
    43381 tgacaactgg agtgattacg ctatagccag ctgctctaat tgcttcgatg tcaaaacgaa
    43441 tcaataaatc tcctgctttg acgagatccc cttgtttgac taagatctca aagccattcc
    43501 catttaattc tactgtatcc atcccgatat gaatcaaaat ctccgctccg tcagtcgttg
    43561 tgatccctac agcatgtcca gttggaaaaa tagtcgtgat ctctccgtct gcaggtgcaa
    43621 ctagttctcc tacttctgga tcaatagcga taccttttcc catcacgcca gaagaaaaaa
    43681 cttggtctgg tacttcactc aatggcagta ctttacccgt taaaggactt tttaaggtat
    43741 gccgtgctga agtaatcggt tctcctgcat tcgcatgttt gttttctaat tgattgtctt
    43801 gtgtttgttc gtcaaatcct aaaatcaatg tacctacgaa tgcaagaaca aaagcaattc
    43861 ctgtagctat tactgctact gtcacatttg actctacacc atccaccgta ttaatgaaag
    43921 tcggaatcgt caataagctg accaaactgc tggagaaagc ttttactccg gaaaatccga
    43981 taatagctcc accaatacct ccagaaatac aagcggcaat gaatggtttc ttcaaaggta
    44041 atgtcacacc atacacagtt ggttcagtga ttccaaataa cgaagtcact gtagaagaaa
    44101 tccctaatgc tcgaagtttc gtatcttttg ttcgcaatgc tacagctaaa gcagccccac
    44161 cttgtgccaa gatggctgga agaagcattg gcatcaatac atcaaaccca tattgttcga
    44221 tattcaaaaa catgatcggt acgaatcccc aatgcatgcc gaacatcacg aatacttgcc
    44281 ataaacttcc catgattaga cctgctacga ttggactaaa tccataaatc gagttgaaga
    44341 gaccgcctaa agcattaccg gcaaccgtcc cgattggtcc taaagcaaga aatgttaatg
    44401 gaaccatcac tactaaaacg acaagcggaa ctaaaatcat ttgcagaaat tgcggaatca
    44461 ctttttttac gaatggctct aatttacttt gaacccatac ggctagaatg attggtatga
    44521 cagaagaagt ataaccagat ggtgaaagaa tcactgggat cccaaagaaa ctgatagctt
    44581 ctcccgcacc agcaactgct gtgatagctg gatacacaag tgccatagca attgctactg
    44641 ctaaaaactg gttggctttg aactttttag ctgctgtaaa agcaagcatg acaggtaaga
    44701 aggtaaagac accatctgct gcagcaaaca atatttgata agtaccgcta tcagctgtca
    44761 gccagcccat caccgttgcc aatgataaga atcctttcaa taccccggcg gctgccatcg
    44821 cccctagaaa aggtgtaaat atactagaga taatatcgat cagacgattc aaaatatttt
    44881 tggactcttc ttttccatcc tccgaaactg caagaccttc ctcatccacg atcgattgat
    44941 aaacatctgc tacattactg ccaatcacta cttgatactg accgccactt tgtacgactt
    45001 ggatcacgtc cgggtcttct tttaactttt gcgtatctgc taaagattca tcttttaatc
    45061 tgaatcgcaa ccgcgtagca caatgtacta aacttttgac attcttttgt cctcctaccg
    45121 cttcccatac acgacgacca actgcttgat tatccattgt tttttccttc tttctttttt
    45181 atttaaaaaa taaaaaccca gattgaaaaa aacagtcaca aacattcgtg aacctatttt
    45241 ttcaatccag gttttgcctg ccatacagta acaatcctta tgattggatg ctttttttct
    45301 cacttactac tctttgaata tgaatcgtta aataaatctg ctctgcgatc cccatttcct
    45361 gtttttgcgt ttgtcgaaga tactcattga tccgttcgac acaatgaaac gcttttggat
    45421 actttccttg aactaatgcg tagagaaatt catcttgctc gtcagatcct tcccctttga
    45481 gataacgctg ggcaaaaaac tgcagatgag taatgatacg ctgataattc aaagattctt
    45541 catcgaacac gatgccgaag aatttactga tgatactcag tatatctctt accattgtgg
    45601 tagcggtcat cgtcacttgc atgtcttcgt ttccttgttg gctattcaca aaatgaaagg
    45661 caataaaacc ggcttcatcc tcagaaagtt ccacacccgt cttttgtttc acgaacaaaa
    45721 gagcctcttt cgctgcttga tattctttcg gataaaattt ttttgtttca aataataatg
    45781 gattaggcag ttggatattt tcttttgctc gtgtgatcgc ataatgaaga tgatcggtca
    45841 atgtcagata aatattaggc gaaaattcaa cttttagttt atcttcagcc agttcgacta
    45901 cttgtttgac ccattcgatc tcctctgccg gaatatcagc catcagctgc tggaattggt
    45961 ttttagcaaa agaatctctt aaaacaaatt ctttttggac cgcttctggc gaaatctcgt
    46021 ccccaatctt tttttgaaaa gctagtcctt ttcccataca aacgacttct tccccgaatc
    46081 catttttcga aatgacgaca ttgttattca aaattttatc aattttcatt ttgcttcgcc
    46141 ctctcaagaa aaataaaaaa acctagaatt cgcctactgt ttcttagctg aattctggtt
    46201 ttgcctgccg tacagtaaca atccatttct tattcaaaat catcatacca tcgaattttt
    46261 atttatgcaa gccttttcat ataaaaaata aaaaaactgg aacacaagtc tatctagaaa
    46321 acaa
//
LOCUS       DACEDD010000017        42403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEDD010000017 DACEDD010000000
VERSION     DACEDD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.017"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IWO45_001093"
     CDS             complement(148..2301)
                     /locus_tag="IWO45_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0332579.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="IWO45_001094"
     CDS             complement(2595..3443)
                     /locus_tag="IWO45_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0332580.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWO45_001095"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWO45_001095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0332581.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="IWO45_001096"
     CDS             complement(5944..6123)
                     /locus_tag="IWO45_001096"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0332582.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWO45_001097"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWO45_001097"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0332583.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="IWO45_001098"
     CDS             8271..10550
                     /locus_tag="IWO45_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0332584.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="IWO45_001099"
     CDS             complement(10643..11290)
                     /locus_tag="IWO45_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332585.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="IWO45_001100"
     CDS             complement(11469..12188)
                     /locus_tag="IWO45_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0332586.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="IWO45_001101"
     CDS             complement(12319..13470)
                     /locus_tag="IWO45_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0332587.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="IWO45_001102"
     CDS             complement(13687..14427)
                     /locus_tag="IWO45_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0332588.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWO45_001103"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWO45_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0332589.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /locus_tag="IWO45_001104"
     CDS             complement(14811..15404)
                     /locus_tag="IWO45_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0332590.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16035)
                     /locus_tag="IWO45_001105"
     CDS             complement(15388..16035)
                     /locus_tag="IWO45_001105"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0332591.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWO45_001106"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWO45_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0332592.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWO45_001107"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWO45_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0332593.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="IWO45_001108"
     CDS             complement(17481..18008)
                     /locus_tag="IWO45_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0332594.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="IWO45_001109"
     CDS             complement(18188..18973)
                     /locus_tag="IWO45_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0332595.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="IWO45_001110"
     CDS             complement(18966..19835)
                     /locus_tag="IWO45_001110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0332596.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="IWO45_001111"
     CDS             complement(19836..21215)
                     /locus_tag="IWO45_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0332597.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWO45_001112"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWO45_001112"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0332598.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWO45_001113"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWO45_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0332599.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWO45_001114"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWO45_001114"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0332600.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWO45_001115"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWO45_001115"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0332601.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWO45_001116"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWO45_001116"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0332602.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="IWO45_001117"
     CDS             complement(25173..25397)
                     /locus_tag="IWO45_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0332603.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="IWO45_001118"
     CDS             complement(25450..26412)
                     /locus_tag="IWO45_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0332604.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="IWO45_001119"
     CDS             complement(26422..26862)
                     /locus_tag="IWO45_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0332605.1"
                     /translation="MESDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="IWO45_001120"
     CDS             27168..28133
                     /locus_tag="IWO45_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0332606.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="IWO45_001121"
     CDS             complement(28190..28420)
                     /locus_tag="IWO45_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0332607.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="IWO45_001122"
     CDS             complement(28506..29393)
                     /locus_tag="IWO45_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0332608.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="IWO45_001123"
     CDS             complement(29482..30705)
                     /locus_tag="IWO45_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0332609.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31801)
                     /locus_tag="IWO45_001124"
     CDS             complement(30722..31801)
                     /locus_tag="IWO45_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0332610.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWO45_001125"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWO45_001125"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0332611.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="IWO45_001126"
     CDS             complement(33659..33925)
                     /locus_tag="IWO45_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0332612.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="IWO45_001127"
     CDS             34404..36638
                     /locus_tag="IWO45_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0332613.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="IWO45_001128"
     CDS             36786..37106
                     /locus_tag="IWO45_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0332614.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37311..37535)
                     /locus_tag="IWO45_001129"
     CDS             complement(37311..37535)
                     /locus_tag="IWO45_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ0332615.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37733..39313)
                     /locus_tag="IWO45_001130"
     CDS             complement(37733..39313)
                     /locus_tag="IWO45_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0332616.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39714..41090)
                     /locus_tag="IWO45_001131"
     CDS             complement(39714..41090)
                     /locus_tag="IWO45_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0332617.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41218..42336)
                     /locus_tag="IWO45_001132"
     CDS             complement(41218..42336)
                     /locus_tag="IWO45_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0332618.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        11990 a         9126 c         7323 g        13964 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttggatct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcagatatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggtagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctgattcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    37201 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    37261 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    37321 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    37381 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    37441 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    37501 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    37561 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    37621 gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga gaggctgttt
    37681 ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt ctttacatca
    37741 aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca aattggttct
    37801 cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga cttgaactca
    37861 tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc attggagaca
    37921 tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg aattgtagct
    37981 gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc tgaatcgctc
    38041 cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg acattttttg
    38101 cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa gcaactttta
    38161 gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat acaccgatga
    38221 tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat
    38281 tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct cgttcgaatg
    38341 ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc atatttgctt
    38401 ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc tgatcatctt
    38461 gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa aaagtctgta
    38521 caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt
    38581 tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct aagacttgtt
    38641 catataagga attttcagtt agaggatgat tttcgggaag cagtccatct tcatccaatt
    38701 ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga ttatggtaga
    38761 tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc ggataagatt
    38821 cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga ccatcacgat
    38881 ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact tggaataatt
    38941 tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca ctatccacgg
    39001 ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta tccaagatat
    39061 ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa atcccacgtt
    39121 gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg ccttttacgg
    39181 tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc gttgttttcc
    39241 cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt
    39301 ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt ttctctaggc
    39361 acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac aaaagccgtg
    39421 tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc aataagccca
    39481 cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact taatgcagga
    39541 tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat ggattcagcg
    39601 aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga ctaaaagaaa
    39661 agacgtgttg cttgtttatt caagcaacac gtctttcata accaagtttc caattatctt
    39721 cttggctcgt catcttcgaa ttcttttctg aagtatcttc gttcttcttc aggttccgct
    39781 tctgtttctt ccggatcatg aaaaatgaca cggattttaa ggattcgaga accttccatt
    39841 tcttcagaaa ccagcgtaat attatcgaca tcaaacgata acttttcgcc ttcatccgga
    39901 atcatgccta atgctgtaat caaataacca gccatcgtat ctacatcact catatgcaga
    39961 tcactgtcaa aagcttcatt gaactcatca atcagcatac gtccttggat gatatattcg
    40021 tgttcatcga ttttttcata aagattctca acttcatctg attcatcatc gatttctccg
    40081 acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa cgacgccgcc atattcatct
    40141 aataaaatag ccatctgatt ctgcgtcttt ttcatttcat ataacagatc atcaataaag
    40201 accgtttcag gaacgaataa aggctcctgc atgattttct tgatatctaa attatcaaag
    40261 ccgaatttat gggcagcttt tagtaaattc tttgtatgaa ggattccgac tactttatct
    40321 ttgtcttcgt tataaacagg gattcttgaa tagttttcgg ataaaacttc attcacattt
    40381 tcttccacag tgtcgttgat atcaatcata aatgcatccg ttcgtgggac catgacttct
    40441 cgggcaactt tcgtatcaag agaaaaaacc ccttggagca tttctaattc ttcattattc
    40501 aatacgcctt cgttttcaag catataacgc atttcatctc tggtcatttt tgaatcttca
    40561 tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg tggaagcaga aagcagccag
    40621 acgaagggct tagctactac accaatcaag cgtatcagcc ctgaagtgaa ctttgcgact
    40681 tcttctgatt tatttaaagc aatacgttta gggtacaatt ctccaaaaac aatcgataca
    40741 taggtcaaaa tagccagcac aatgatattt gcaatatttt ttgccgctgt gccgccacct
    40801 aataccggag ctaaccgttc cgataaagta ttagctagtg aagcaccaga caaaatgttg
    40861 accaaagtga ttcctacttg gattgtcgat aggaaattat ttggattctg caatattttt
    40921 aaaagctttt gagatttcac atctccttca tcagcttttt gctcgattcg attcttattg
    40981 atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat tgatcaatgt caaaacgatc
    41041 aataataaaa tctgcgctaa cagcgactga ctctcagggt cagcattcat aatcgcattc
    41101 tctccttata aaattaattt ttagtttacg atagaaaaaa acgtggcact atagccacgt
    41161 taaaatggta tcacaaagca attttctttg caataaaaac aagtgtacta aaactaccta
    41221 cgaggtcgta gcaattttct tcttgttttc attttgcttg tcttctttga cgattttgat
    41281 tatataggaa atgatcgtct tgcagattgc gtaaaaagga acacctaaga agataccaag
    41341 caatccggca atatttccag ctactagaag aacgataatg atcgttaatg gatggatttt
    41401 cagtgatttc ccaattacat taggataaat gatattccca tccaactgtt ggacgattag
    41461 cacaaccaca caacaaagta atgcttttac cggatcattg aagactgtta ctagaaatgc
    41521 aggtgctagt cctagataag gtcctaaata tggaattagg tttgtcaatc ccgcaatgac
    41581 cccaaataag aaagcataat ccacaccaat taccatatat cctaaaaatg tgaaggtacc
    41641 aacgaataaa cattcgattg cttgtccgct aatgtaatta gccaatgttt tattcagttg
    41701 ccccaataac tccacaattt ggtttcttct tttttctggg aaaaactgtt ggatgtttgg
    41761 cactagtttt tctccgtctt tcaacatata gaacaagatg aatggtgctg taatgattac
    41821 tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa ctattggata ctccactaag
    41881 gaattgctga atgattgtcc cgtaagaaat gtctaattgc tcaaaatatt tattgatatc
    41941 tacttccttg aacaaaggca attcggctac ctcttttaac cagctttcta cctgtttgat
    42001 gaaatcaggc atattagatg ccaatgagga aatttgctgt actagacttg ggatgacact
    42061 aagtaaaatc caaataattg ctgcaactaa aaggagcagc acaattgcga cagcccatat
    42121 tcttttgatt tttgctttca tcaacagctt gacaatagga ttcaataaat agtaaagaaa
    42181 gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat gtcccgatcg gttcgaaaat
    42241 aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg atcaataatt caagcgacca
    42301 aaacattagt ttagactgtc taagtttttc aaacaataga tttcctcctt tctttcttac
    42361 tagtatatat tatattcgtt aaaaattctt ttattctttt ttt
//
LOCUS       DACEDD010000018        42297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEDD010000018 DACEDD010000000
VERSION     DACEDD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.018"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            397..1260
                     /locus_tag="IWO45_001133"
     CDS             397..1260
                     /locus_tag="IWO45_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0332619.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1389..3011)
                     /locus_tag="IWO45_001134"
     CDS             complement(1389..3011)
                     /locus_tag="IWO45_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0332620.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3260..3781
                     /locus_tag="IWO45_001135"
     CDS             3260..3781
                     /locus_tag="IWO45_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332621.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3967..5697)
                     /gene="spxB"
                     /locus_tag="IWO45_001136"
     CDS             complement(3967..5697)
                     /gene="spxB"
                     /locus_tag="IWO45_001136"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0332622.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5862..6680
                     /locus_tag="IWO45_001137"
     CDS             5862..6680
                     /locus_tag="IWO45_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0332623.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6783..6980)
                     /locus_tag="IWO45_001138"
     CDS             complement(6783..6980)
                     /locus_tag="IWO45_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332624.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7149..7727
                     /locus_tag="IWO45_001139"
     CDS             7149..7727
                     /locus_tag="IWO45_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0332625.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8772..9275)
                     /locus_tag="IWO45_001140"
     CDS             complement(8772..9275)
                     /locus_tag="IWO45_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0332626.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9634..10659
                     /locus_tag="IWO45_001141"
     CDS             9634..10659
                     /locus_tag="IWO45_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0332627.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10982..11860
                     /locus_tag="IWO45_001142"
     CDS             10982..11860
                     /locus_tag="IWO45_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332628.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12336..12452
                     /locus_tag="IWO45_001143"
     CDS             12336..12452
                     /locus_tag="IWO45_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0332629.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13380..13718
                     /locus_tag="IWO45_001144"
     CDS             13380..13718
                     /locus_tag="IWO45_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332630.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13812..14042
                     /locus_tag="IWO45_001145"
     CDS             13812..14042
                     /locus_tag="IWO45_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332631.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14442..14813
                     /locus_tag="IWO45_001146"
     CDS             14442..14813
                     /locus_tag="IWO45_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0332632.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15087..15779)
                     /locus_tag="IWO45_001147"
     CDS             complement(15087..15779)
                     /locus_tag="IWO45_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0332633.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15782..16567)
                     /locus_tag="IWO45_001148"
     CDS             complement(15782..16567)
                     /locus_tag="IWO45_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0332634.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16804..18204
                     /gene="lacG"
                     /locus_tag="IWO45_001149"
     CDS             16804..18204
                     /gene="lacG"
                     /locus_tag="IWO45_001149"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0332635.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18229..18546
                     /locus_tag="IWO45_001150"
     CDS             18229..18546
                     /locus_tag="IWO45_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0332636.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18559..20232
                     /locus_tag="IWO45_001151"
     CDS             18559..20232
                     /locus_tag="IWO45_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0332637.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20246..21877
                     /locus_tag="IWO45_001152"
     CDS             20246..21877
                     /locus_tag="IWO45_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0332638.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22749..23939
                     /locus_tag="IWO45_001153"
     CDS             22749..23939
                     /locus_tag="IWO45_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ0332639.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24073..26262
                     /locus_tag="IWO45_001154"
     CDS             24073..26262
                     /locus_tag="IWO45_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0332640.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26259..28052
                     /locus_tag="IWO45_001155"
     CDS             26259..28052
                     /locus_tag="IWO45_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0332641.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28252..28566
                     /locus_tag="IWO45_001156"
     CDS             28252..28566
                     /locus_tag="IWO45_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0332642.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28587..28964
                     /locus_tag="IWO45_001157"
     CDS             28587..28964
                     /locus_tag="IWO45_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0332643.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28974..29747
                     /locus_tag="IWO45_001158"
     CDS             28974..29747
                     /locus_tag="IWO45_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332644.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29769..31172
                     /locus_tag="IWO45_001159"
     CDS             29769..31172
                     /locus_tag="IWO45_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0332645.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31354..32538
                     /locus_tag="IWO45_001160"
     CDS             31354..32538
                     /locus_tag="IWO45_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0332646.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32535..32825
                     /locus_tag="IWO45_001161"
     CDS             32535..32825
                     /locus_tag="IWO45_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332647.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32822..33043
                     /locus_tag="IWO45_001162"
     CDS             32822..33043
                     /locus_tag="IWO45_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332648.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            33085..33864
                     /locus_tag="IWO45_001163"
     CDS             33085..33864
                     /locus_tag="IWO45_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0332649.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            33931..34425
                     /locus_tag="IWO45_001164"
     CDS             33931..34425
                     /locus_tag="IWO45_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0332650.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34481..35119
                     /locus_tag="IWO45_001165"
     CDS             34481..35119
                     /locus_tag="IWO45_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332651.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            35191..35586
                     /locus_tag="IWO45_001166"
     CDS             35191..35586
                     /locus_tag="IWO45_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0332652.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35570..38023
                     /locus_tag="IWO45_001167"
     CDS             35570..38023
                     /locus_tag="IWO45_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0332653.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            38020..40047
                     /locus_tag="IWO45_001168"
     CDS             38020..40047
                     /locus_tag="IWO45_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332654.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            40044..41066
                     /locus_tag="IWO45_001169"
     CDS             40044..41066
                     /locus_tag="IWO45_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0332655.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            41083..42012
                     /locus_tag="IWO45_001170"
     CDS             41083..42012
                     /locus_tag="IWO45_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0332656.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT        13844 a         7012 c         8359 g        13082 t
ORIGIN      
        1 ttgttccttg tgactcggag ctaaacggca ggctcacaac ctcttggtaa acggtgttca
       61 atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact attttttccg
      121 tttttttctc taatactcag gtcaaaacgc tgatttcaca acctctgatt cacggtgttc
      181 cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
      241 taaattcctt cttatttctt gaagctgacc acttctgtca caacctctta ttttgattag
      301 aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct ataatagacg
      361 ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt aacagattct
      421 tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt atcaatgaat
      481 ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa ttatgaccgc
      541 tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa tgttggccga
      601 tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata tgtggctttt
      661 tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt gctgaaggaa
      721 gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat tggggaaggg
      781 cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga agaaacccta
      841 gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga cgatctgaaa
      901 tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag tctattgagt
      961 gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga caaagttcga
     1021 ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat cgttgcacca
     1081 cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga gaaggcaaaa
     1141 cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt aggaccaacg
     1201 atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat cagtcgataa
     1261 aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa cagcgactct
     1321 taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt tttaagctaa
     1381 ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt ttccaaaaat
     1441 tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc aatgatgcgg
     1501 tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga gccagtaaat
     1561 gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt cgttggatca
     1621 tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca acggactttc
     1681 tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa caacattctt
     1741 cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata ttgacgcaac
     1801 cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg taaataagaa
     1861 ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc acccatgata
     1921 actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac cttgtcttct
     1981 ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt tacacttaca
     2041 ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc aacgaatgga
     2101 taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat ttttttacgt
     2161 gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa ttcttggagt
     2221 tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc cgctagttga
     2281 ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc aaaatctaga
     2341 tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac aacgatcaca
     2401 gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat atccagaccg
     2461 tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg tgctaataac
     2521 acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa ttcttcaggg
     2581 atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc attaagctca
     2641 gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa atcctctttc
     2701 atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc catgatgact
     2761 gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac ggccatccgt
     2821 tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga taacacttta
     2881 aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt tccgggggta
     2941 aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact cacatcattt
     3001 acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt cttatagaat
     3061 tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt tcacgtgaaa
     3121 cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg atttattagt
     3181 ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa ctgtaaaaga
     3241 aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct cattgatttt
     3301 gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg gaaatttagt
     3361 tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg aactgtttgt
     3421 acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa taatcgggat
     3481 catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag tcgcagcatt
     3541 gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat ttactgctga
     3601 cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca atattttagg
     3661 aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga cattaagctt
     3721 tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg catttagata
     3781 agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt tgtaattgga
     3841 agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt cgagacaaat
     3901 ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg aagcatagcg
     3961 cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat ggacttcata
     4021 tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta aatgcagatc
     4081 ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag gacgatctga
     4141 ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg taaagctatt
     4201 tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata caccaaattt
     4261 cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat tcgaaaagac
     4321 aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca tgaccatagc
     4381 aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta gttgtgcggc
     4441 aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg tatgtttttg
     4501 tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga catctgtgac
     4561 gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct ctggacgaat
     4621 cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt ctttattttc
     4681 ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga caagtctttg
     4741 caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc gtccaaattt
     4801 tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg cacgtccaaa
     4861 tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt catttccggg
     4921 tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag gaataattcc
     4981 ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt ctttgatttg
     5041 cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg ctttttcaag
     5101 ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg ttttgtgagt
     5161 gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa cagggatagt
     5221 cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta ctacatgcgg
     5281 aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga aaataggatt
     5341 ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt gtcctaagaa
     5401 cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca aatgtgtcgc
     5461 accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg catctgcagc
     5521 ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt cggttttttc
     5581 ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt aaatatggtc
     5641 gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt ttgacatggt
     5701 gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta tagaactttt
     5761 tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt ttgggaatat
     5821 gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa tggctattga
     5881 gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt atgacagctc
     5941 ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta aacttgatgt
     6001 taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta gatccggcta
     6061 ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt gggtttggtt
     6121 taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat tttggaccac
     6181 aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt gttttttcta
     6241 ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt gcagcagcaa
     6301 ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta ggtattggta
     6361 tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa ggtctgagtc
     6421 gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt gctggagtga
     6481 ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt gcttttgccg
     6541 caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag cagacattgt
     6601 ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt atgatgattt
     6661 tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata gggccgcgaa
     6721 cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa tggtatcata
     6781 cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag ccagatgata
     6841 agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag gacactgaca
     6901 aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa gatatttaag
     6961 aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact atagaaaaag
     7021 tataacaggg attgtttaaa aacacctcca actgaagact gatttttact tcatctactt
     7081 aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg gaaaaggagg
     7141 agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc gcgattatca
     7201 tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag gcgctcgctg
     7261 cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg atttattgga
     7321 acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc ttatgggcga
     7381 acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg atcagtgagt
     7441 acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc gctaatattg
     7501 cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag aaagcattga
     7561 aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat gtgttaggaa
     7621 ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc gtgattctag
     7681 ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat gatccgtgtt
     7741 taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc gagtgatact
     7801 agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact gattactagt
     7861 tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt agttggtgtc
     7921 gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg taatctgatg
     7981 ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa acgtgttttt
     8041 atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt agtttttcca
     8101 cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg cgtatagatt
     8161 tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt caaacagtat
     8221 aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct tttgttattt
     8281 tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta ctacttctaa
     8341 aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca gaatatactg
     8401 gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat ataaagtaat
     8461 tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta taaattaaga
     8521 cggaattcct ttctagattg agatattgac taggaagaat gttaagattt taagccttta
     8581 agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt ttcaaactgc
     8641 aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt tttaaaccaa
     8701 tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac ggtctttttt
     8761 tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga
     8821 aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt tgtcgataga
     8881 tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt attagttatc
     8941 aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc tgccaagaag
     9001 cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa attgacattc
     9061 aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg taataccgtc
     9121 gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta agaataagtg
     9181 agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag aaagaataaa
     9241 taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat ttaaaaagta
     9301 taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac caacgtagtt
     9361 atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa caaagctaag
     9421 atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc ggaatgttcg
     9481 ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat gaaaaatcaa
     9541 gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat cttggttcag
     9601 ctttttttcc aattactagg aaggagggat agattgttca agacttattt tcgtttcaat
     9661 aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt tattgtatta
     9721 tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt agggatcttt
     9781 acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat gtttctgatc
     9841 ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt tttatggctt
     9901 gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg attattccat
     9961 atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac gagcatgcag
    10021 ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt cgtttttgcc
    10081 ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac aattgaaaaa
    10141 gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc tgttttctac
    10201 agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca taatccttat
    10261 tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc gatgaagcat
    10321 cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc attcttgtca
    10381 tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata tggaatcgca
    10441 caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc gatggcgttg
    10501 atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata tcgtttttta
    10561 gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac ggttggtttg
    10621 caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata tatgtgccaa
    10681 aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa aagccagatt
    10741 ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa aaattttata
    10801 tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc tgagatggtc
    10861 aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta agttttatag
    10921 ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag tcatttaaac
    10981 aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca atgaaatcca
    11041 aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga ttgtacaagc
    11101 aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg atcctaataa
    11161 agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt ttgcagcatt
    11221 agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac cattgaagat
    11281 tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc atgaacattt
    11341 ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt acactagtgg
    11401 cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga aagggtacac
    11461 ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg atgcagattt
    11521 atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg aactttattt
    11581 tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac ccaatcttac
    11641 taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc gattattatc
    11701 agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag atgcccaaat
    11761 acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat tgcttgcagg
    11821 cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact tcgaaatagt
    11881 ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
    11941 acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag
    12001 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
    12061 ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa caatagatat
    12121 taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat gaaaatttat
    12181 ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt tgactttcat
    12241 tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt gttttttagc
    12301 atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat atctcaagtg
    12361 cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg aagatcatca
    12421 aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt aaaaaaatag
    12481 ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt tccgaaagaa
    12541 ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc ttttcaaatt
    12601 gataccattc cctaattcca gttatatagt taactacaga accacaaagc acgtacgttt
    12661 ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt ttgaccagta
    12721 gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt tagtcaagaa
    12781 tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata tttcatgtag
    12841 aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac gaaagctccc
    12901 aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag tgattcgtat
    12961 ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa tatgcaaaaa
    13021 aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt ttggttttca
    13081 gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt atttttcatg
    13141 aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt aaggaggaac
    13201 ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag actcaaagtt
    13261 gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga agggcttttt
    13321 tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta acattttcta
    13381 tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca gaagagttta
    13441 agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca attactgata
    13501 ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta aataatgact
    13561 caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg agttcattgt
    13621 taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg gattactcaa
    13681 aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa tactttatga
    13741 tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc aaaagcagga
    13801 agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg aatgtagcaa
    13861 aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca attaactcga
    13921 ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt tggactaaag
    13981 aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt atgcaacagt
    14041 aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt ttcgatatct
    14101 ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta ttttatttta
    14161 acttttgtat atttagtaag tagctacaca cttctatata aattcagaag ttcttctaga
    14221 gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg atttatatag
    14281 gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta atagatttaa
    14341 taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac cctgtagaag
    14401 aattttttca agattacttt gcggagtatg ccgataggat aatgagagat tacgtaacag
    14461 ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct gataaggcga
    14521 aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat atgagtggtg
    14581 aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt atcaaaactt
    14641 ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa ttaattaata
    14701 tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg ttgtttacag
    14761 ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt taaccattac
    14821 gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa gattggtttg
    14881 agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga acgcgtcata
    14941 tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg atatagcagt
    15001 gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca tttcagttgc
    15061 ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga caggtgctct
    15121 gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc ctgttcaaat
    15181 actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc cgcacgtttg
    15241 gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg agctaagctc
    15301 agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa agagccgtcg
    15361 tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc aaaataaaaa
    15421 gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg gtcagtcagc
    15481 tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt ttgcaagcaa
    15541 agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga ggcggtcaaa
    15601 tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat attttttaaa
    15661 atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag ccaagcagct
    15721 tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat ccctttcata
    15781 gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg acatattgtg
    15841 catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga tcgaaatcat
    15901 gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca acgagtagat
    15961 atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg gtactgtaag
    16021 tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta aactttagtc
    16081 ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc cggaaatcac
    16141 cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa actggataat
    16201 tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt tcgattgttg
    16261 tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct gtggcagcaa
    16321 tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt tcttgatcta
    16381 aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc gatttttcta
    16441 attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg atgatttcat
    16501 taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat ctttcttttt
    16561 tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga caacagtcaa
    16621 tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca atagatttta
    16681 ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa tgttttggat
    16741 gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg aggagagatg
    16801 tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc ctatcaggtt
    16861 gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt tttagaggaa
    16921 caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc aaaggacatt
    16981 gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg gagccgcatt
    17041 tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca tcgtgtgttc
    17101 gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt tgatacccct
    17161 aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc atttgtttcc
    17221 tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac ctttaatgaa
    17281 atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg aatcaaatat
    17341 gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc ccgtgtagtg
    17401 aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc attagagacg
    17461 aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga cgatgcattg
    17521 tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga aaccttgact
    17581 gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga agaagatttc
    17641 gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca ctaccaatgc
    17701 cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac gggtgaaaaa
    17761 gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga aggaattcct
    17821 cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt gctgcgaatt
    17881 aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat ggggtataaa
    17941 gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt gagagtctat
    18001 ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta ttttttgtgg
    18061 tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg cttgttttat
    18121 gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta caagttggtc
    18181 agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat gaataaagag
    18241 gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg cagtacatta
    18301 ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc ggcgttgaaa
    18361 gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt agcggaagaa
    18421 gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga tcatttaatg
    18481 accactttat tgctgagaga tctgattcaa gactttattg tactgtacag acagaatcaa
    18541 ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg gaaaccattt
    18601 tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt tttaacagcg
    18661 atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc tgagattttt
    18721 ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa ttattcgatg
    18781 ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga ttcgatgaat
    18841 cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc ctctgtggta
    18901 agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga atatatgggg
    18961 acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt atataagttt
    19021 tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac gatttcgcaa
    19081 accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt aattgattta
    19141 ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt gctgcagccg
    19201 ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc gatggcgctg
    19261 ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc ggcaattatc
    19321 tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc ttctaaagta
    19381 ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc aacattggtt
    19441 gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt tgggaaagct
    19501 tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc acccatgatt
    19561 ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt ttgcctgttc
    19621 aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc ttgggcaact
    19681 ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt tgttctagca
    19741 gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc atacgatgct
    19801 gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga aggtgcttct
    19861 gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga tgcaaataaa
    19921 gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt agctaatgct
    19981 ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg tgcttatggt
    20041 tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca agtcaattct
    20101 tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt tgcgacaaaa
    20161 ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt tattctgaac
    20221 caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta aagctgtttt
    20281 atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag catctgccag
    20341 cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat ctggtgcaaa
    20401 atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata atggtgtcaa
    20461 ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa gttatggagc
    20521 agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa atctgggact
    20581 taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga aacagaatct
    20641 tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt acgactatac
    20701 aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg ttcagatcgg
    20761 caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag aaggcggggg
    20821 agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc gagacactca
    20881 acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag gagcatttac
    20941 ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg gagtgtcgta
    21001 ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg cgatcagcca
    21061 acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa cagcgaatgc
    21121 cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat atgaaccaac
    21181 accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg cagtaccgaa
    21241 taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa atgtgagttg
    21301 ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa acgaatggga
    21361 caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg cgtttaaaca
    21421 agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg gaccaacgac
    21481 cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta cgatcaaaac
    21541 ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc aagcatacga
    21601 ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat tgagtgccta
    21661 tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt atggtggagc
    21721 ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa tcaaagacat
    21781 tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag gaaatgcttg
    21841 gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt tcgattcaag
    21901 atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa cctgcacctc
    21961 gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac gagtaaagca
    22021 ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc gtaagaagca
    22081 atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa agggtaacct
    22141 tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag ttctcggaaa
    22201 acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta acttatatta
    22261 tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt ttagctattg
    22321 aagaatgttg atttataggg attccccctc catattatgt tggctcttta ttcccactct
    22381 gttttcaacg taaagtgtat aaaacagaat gcgatactat caagggagtt aaaacaatat
    22441 acagaacagt aaaacactat aaataaaaat aaaagtgcaa taaaagtgca atttttgcac
    22501 ctaatcatac tctgtttata tatgaaaact agattctgaa tccaatattt ggttaacgag
    22561 aggcgttcga atttgaacgt cttttatata ttactttcaa ataaatatga ggtagaatac
    22621 tatctatttt ctttttaacc caattgttat aatggagagt gtaaaatatt ttgtgtaaat
    22681 agaaaaaagg aagtcccttc tgtagaatag agttaccaca acacattcac agaagagagg
    22741 acttccctat gaacgatttt actacagaaa ttctaaagac tctagcgaac aaaggcgatt
    22801 tgaatgaatt attccgtgtc catttggaga aagcagtcaa tacgcttctc aaaacggagt
    22861 taacggcttt cctagattac gaaaagtatg atcgcattgg ttttaacacg ggtaattctc
    22921 gtaacggctc ctatgaccgt acggtcaaga ccgagtatgg ggaacttcat ctccagattc
    22981 cgcgcgaccg caatggtgag ttcaagcaac agactgttcc tgcttataga cggacgaatg
    23041 acacgttaga ggagaccgtc attcacctct tccgaaaagg tattaccatg tcggaaatcg
    23101 cagacttgat tgagaaaatg tatgggcatt actacacgcc ccaaaccatg tccaatataa
    23161 caaaatcatt tacagaagag gtaacggcgt ttaatgggcg ggagcttcat gaccgttatg
    23221 ctgctattta tatggacgca acgtatattc cgttaaagcg gaaaaccgtc gccaaggaag
    23281 ctattcatat cgcagttggc attcgcccgg acggatcaaa ggaagtattg agctatgcga
    23341 ttgcaccgac tgaatccatc acgatttggg aggaaatttt attggacctt caagagcgcg
    23401 gtttgaaaaa tgtcctcctg ttcatcacgg atggcttaaa ggggatggta ggagcgatta
    23461 gtcggttcta tcccaaagct cgttttcaac attgttgtgt acacgtttcc cgtaatatca
    23521 gtcataaagt gcgtgtcgat gatcgtaagg aagtctgtga tgattttaaa atggtgtatc
    23581 aagcctcatc taaagaggtg gcgttggaag cacgtggtac ttttgcggaa aaatggaaaa
    23641 ccagctatcc aaaagtggtt gaatcgattc tttcgaacga tcacttgctc actttctatg
    23701 atttcccctt ggccatacgc aagagtattt attctacgaa cttgattgaa tcctttaata
    23761 aacaaatcaa gaaatacagc caccgcaagg aacagttcca aaatgaagag tcgatggaac
    23821 gtttcttagt ctcgtctttt gatacttaca accaaaaatt cctaggtcgc agtcataaag
    23881 gtttccaaca ggccgaaggc gagctcgaac aaatgctaag ccaactgatt gagaattaga
    23941 aacaatctgc agtagggaag aatcatttac acaaaattat tgacgctccc ttataatgag
    24001 aacttttact aacataattt atgataaact tactatataa tcatattaat taaatagtta
    24061 ggagatctgc ttatgtttat atctactctt caaattcgaa attttagaaa ttatgaatcc
    24121 gaaatctttc gattcgataa tgagacaaat actattatag gtgagaatga ttcagggaaa
    24181 accaatgctc ttactgcatt gagaatctta ttagatgata gttattacta tagtagtaaa
    24241 acactaaaag aatcagattt ttttcatgga attcaaaatg ggtggcaagg gcattggatt
    24301 attatctctg cgacatttga aggaatcagc gaagaagaat ttgacaatga gatttgtgct
    24361 tccatttctt taaattctga aagtcaaaca atcttagaag aattgatttc aaatgcagat
    24421 aaaggtgtag gtagtatttc tctatttatt agacctaaca aagctattag aaagcaatta
    24481 tttgatattt ccactgaatt agactctcat caatttaacg aatttagaaa ttctattcgt
    24541 ctatccgact atgaatttta ctacacatct aaatcaaatc taaatttctg tatcaatgaa
    24601 aattatgata aattggtagg aagactcaat gacaatatag ctagtaatcc tgaagaagac
    24661 gatgaagctc tgttgggctc aagaattgac atgtcagata ttttcaagca tatttcggtt
    24721 gtttatgttg acgctcttcg agatgttctt agagaaatga aaaacaatcg aaacccagtc
    24781 aaaagaatca tggaaacaat tgaatcaaaa atatcatcag ataatgttga gtcattaaaa
    24841 acaattatac agcaattaaa tgagactatt accagtgttc ctgaaattag gaaaattggc
    24901 gaaaatatta atcggcaact aaatagcata attggttcag tgtattctcc taatcttctt
    24961 ctttcctcaa ctatgtcaga tgatatgggt tctttagcaa aatttctttc aatgaagcca
    25021 gaacaaaata tcgacttaga cttactaggg ttaggacatc taaatatgat ttatttagct
    25081 ttgaaaatag tcgaatttga agcgtgtcgt tcaagagaac tacttaatat tatgttgatt
    25141 gaagaaccag aagctcatat tcatcatcat attcaaaaaa ctctgttcga aggattaaat
    25201 cttcaaaaaa actatactca gattctcatg accacacatt ctgtacattt agctgaagtg
    25261 tctgaaatat ctcggatgaa tgtattaaaa tcgtttaatg gcaaatctat cgttatgaat
    25321 ccagcaaata atctgaactc atttgctaaa gaaaagttaa aaaagaacaa tctaaatcta
    25381 ataaacgcag tagagagata tttagatgtt aaaagaaatg gattgctttt ttctaaaggt
    25441 gttatcttag ttgaaggaga tgctgaagaa atacttattc ctcaaatagt aaaaaaatta
    25501 tttggtataa acttagacga acttggaata ggtttgatta atgttggtag tacttcgttt
    25561 gaatatatcg ctagcttgtt tgacgatgat agaatacaga gaaagtgtgc tattgtaact
    25621 gatttagatg aacaggcaat cccatcagaa catcgattgt ataaaagcac tgcacaagag
    25681 aaaggtcaag caagaaaaga aaaattatcc tcccttttta atgaaaacaa ttgggttaat
    25741 tgtttttatg ctaatacaac atttgaaatt gagtttttag atgtgaataa agaaaatatt
    25801 ctaacttatg tcagtccaat tatagatgac gcattcaaag ataaaaaaac aataactgct
    25861 tataaagaag aaatacaaag tgaacaatat ttagaaagaa atttagcgat gttaaaacta
    25921 gccaatcatg ttggtaaggg gtggtttgca attgagttag gtgaaaaaat tgacttttta
    25981 atcaaaattc ctaagtatat tcttaatgct attgcttttg catgtcagga aatagtagat
    26041 ctctcaattt ataaaaaaat tctactccat acaatcgact tgtacgagga aactacagaa
    26101 ttgtcagctt tatcagctag tttaaaaaca agtgatacag ctgatttgaa tatagcaata
    26161 atttctttat tagagtactt tggtaaagat gaggatgttg tgtatctaat caatcagata
    26221 gaaaattaca caaaaatact ttatgctgag gtttcaaaat gacaaatatc ttcgaaaaaa
    26281 tcgtaaatga tttaaattta gaacaaaaag atgttgtgga aagtgaagat aacctttatg
    26341 taaatgcatg tcctgggagt gggaaaacaa gggtacttac aaggaaaatt gcttaccaag
    26401 caattaaaca cgatggatca ctaaaaagaa taattgctat cacatacact aatagagctg
    26461 ctgaagaaat taaggaacgc ctaagtttac ttagtataga tgatgatatt aatatttggg
    26521 tggggacaat acatcagttt tgtcttgaat ttattattta tccttttaaa atgaatctct
    26581 caagagtatc aaaaggattt acaattattg atgattatac tcaaagagct tacgtcagcc
    26641 aaattaataa tatgctgaat ttaaatctca aaacgtatga actgaacaaa attgatttgt
    26701 cactttctca tgattttaaa attaatgaag ttcagtatac tcaagtttct gaaatgtatc
    26761 acactatgtt ggaaaaaaat aaagaaattg attttgatat gattttacta gtagcgtata
    26821 aaattttaac tgtaaacagt aacgttgcac gtaatattgc tagtaatata agggcgattt
    26881 atgtggatga atttcaagat actagagaat tacaatacaa tgtaatagct aaattacttc
    26941 aaaacaaccc tcaaattcag tcaatgtttg ttggagatat tgaccaagca atttatggat
    27001 cattagatgg tatagctaaa tcagttgaag aactagaaat gctaactagt cacacttttc
    27061 aatcaaagac gctacatggc tgttacaggt ctaatcagag attagtagac ttctactcta
    27121 attttcagag ttgtccttac gagattgaaa gcagagggaa taataaaaac gaccgtggtt
    27181 ttataagtta tgattgcaaa gttactaagg aggatttacc caacataata aaaaatataa
    27241 taaaggaaaa gattgaaagc ggcattcatg aaaaagatat ttgtgtactt gctcctcaac
    27301 actatcctct gttttcactg ggtgagtatt taaagaaaga attaccgtat tgcaattttg
    27361 atgctataaa tatatcacca attaaggtaa ataatcatag tttatttttc aagttagcaa
    27421 ttttgtactt tactgaatca ggtgaaaagg ttcgaagaag aaagctaatt gcaaacgata
    27481 ttttaattat attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag
    27541 atttattaca gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg
    27601 atttatacaa atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt
    27661 acccgtctat ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa
    27721 ataattcatt atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta
    27781 tcacacttac tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta
    27841 atcttctaaa cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa
    27901 ttagttcagc gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc
    27961 ttttctcaga ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat
    28021 tagataaaat agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata
    28081 tagtaggaat cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag
    28141 tgaacttatg aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat
    28201 cacacaaata agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt
    28261 aaatatgtca ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg
    28321 aacattgaac aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta
    28381 tactcaacga ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag
    28441 aaacattttg aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt
    28501 tataaaattg gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa
    28561 gcataagaaa ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag
    28621 accagacatt tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg
    28681 aagatggtac ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac
    28741 aaggacgcat gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca
    28801 acgcagaagt ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag
    28861 gggcagacgt tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac
    28921 ctcatcagaa atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa
    28981 acacaccatt gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat
    29041 accattattc aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag
    29101 aaaaattatt aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca
    29161 aactctttcc cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt
    29221 ttcttccaga aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta
    29281 agtggttgaa agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg
    29341 gaaaatgtgg ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa
    29401 gtgtctatcg tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat
    29461 tagaggaaaa tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt
    29521 gcgaatacaa agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga
    29581 caaaagaagc gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag
    29641 aaaaaaattt acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac
    29701 caacttttga taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga
    29761 ggatttttat gccattgctt catgacagag gagagcgaat caaagccaac gataagcact
    29821 tggtctatca ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt
    29881 ttcattggag agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct
    29941 atcaacttca tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac
    30001 ttatcagctg gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc
    30061 aaaagttagc tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag
    30121 agggattctt taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta
    30181 aaatatatta tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga
    30241 aatatcaaga tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat
    30301 tagtggaaaa aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg
    30361 gcaatcgaat tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat
    30421 cggtctattg ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg
    30481 gaaaatccta ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt
    30541 atattcttga ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct
    30601 attaccgtaa agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc
    30661 gcagtgaaac catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg
    30721 gtttgtctgc caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc
    30781 gtgaaagtac caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag
    30841 gcttcttttt aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc
    30901 gggacaactt taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga
    30961 tgttcggaag cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg
    31021 tggatacagg aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg
    31081 atttcttagg agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg
    31141 aagtgaaagg tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct
    31201 ttagccccct ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca
    31261 accaatgttg atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat
    31321 agtttacatc ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag
    31381 gaattgaaag aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct
    31441 catattacca gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag
    31501 gtgaaagaag atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg
    31561 ttattcgact atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag
    31621 gttttacgtt tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc
    31681 gaacactatc gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt
    31741 ttattagaac tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat
    31801 cggagctggt atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg
    31861 gatttagcta tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt
    31921 aaacaggaag aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg
    31981 gttcaccgtg atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt
    32041 gaagtctatt tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc
    32101 ttagaagacg cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc
    32161 caagccatga aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc
    32221 aatcgctata tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact
    32281 aatgaacgtt gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag
    32341 ccagagcctt attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca
    32401 ttaaaaatcg caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt
    32461 caggaagcca aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg
    32521 gaggatttga ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg
    32581 gcattgagtt tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg
    32641 gaaaacggca agctggtgat accatctata cctatgattt ttacaaggtg agattttata
    32701 ataattatac caatcgagta accgtctatg gagagcattt aacccctttt caattattaa
    32761 ggcgtgtaaa aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca
    32821 catgaatttt gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt
    32881 gttagcgatt gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat
    32941 tggcttcttg attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa
    33001 agatgtgtta ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat
    33061 aaaataggaa gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa
    33121 tttaaatctc ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga
    33181 ggtaacgaga ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat
    33241 tttacttatt ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata
    33301 agagttcctt ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca
    33361 gcaagctcca tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca
    33421 cttccagaga attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca
    33481 caagctattg aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac
    33541 agagttcaca ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca
    33601 gttcctagag acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa
    33661 gaaattcaac ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga
    33721 atgattagct ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat
    33781 attattggaa agagagaagc aaaactaata ataggtattt caagtacagt tacagattat
    33841 attagcggat accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt
    33901 tttataaaaa ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg
    33961 tataatgaag gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg
    34021 gcagaaagaa tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt
    34081 ccttttgatg tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt
    34141 caagaaatcg aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt
    34201 agtagcttgg aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat
    34261 tcgatggaag atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct
    34321 tccaatttac aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt
    34381 aattacctag ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg
    34441 ttataattaa attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa
    34501 tcttaaacta ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt
    34561 tcgttacaaa cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt
    34621 tccagaatat gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct
    34681 agctgtctat gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt
    34741 tggtatcgaa tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc
    34801 tcgatttatt ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg
    34861 tttctcatta agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag
    34921 aagaaatcct ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa
    34981 aacgagattg tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt
    35041 cagatattca atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac
    35101 aaaaaataaa attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt
    35161 tttctatttt tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt
    35221 tggtcagtag aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc
    35281 tttacgcaaa tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta
    35341 ccaccacttt ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc
    35401 actgggttca tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct
    35461 atcattacct atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa
    35521 aaagaaacaa tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc
    35581 gattaaatat atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta
    35641 tgaattaatt ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga
    35701 taatttctgt caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc
    35761 aaccgaatca agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt
    35821 aaaagagata gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg
    35881 tgacaatcaa gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga
    35941 agtatccgta aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg
    36001 tgtgaatcac aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt
    36061 ttctaagatg gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt
    36121 ggagaaagat gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt
    36181 tgatgactac gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa
    36241 atacgatatt ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat
    36301 tgaacaggaa gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga
    36361 attggatttc cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt
    36421 tgataccagt atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa
    36481 taaaaagaaa gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag
    36541 tactaatgtg gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac
    36601 caaagaatct atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga
    36661 gctgaaacaa cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt
    36721 acgacctttt ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata
    36781 tatcaatgac tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc
    36841 tacacaaatg attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag
    36901 aaatatttat ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc
    36961 attagcgtcc gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct
    37021 ggtctattat tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg
    37081 tggtgaatgg aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac
    37141 gagtgagaac gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga
    37201 ttcagaaagc cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag
    37261 cgagaagttt cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg
    37321 gttgttattg gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc
    37381 tgaccatatc gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag
    37441 tgtcacccaa tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt
    37501 cttacccgat tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc
    37561 aatgctgatt gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt
    37621 taaaattgtt gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct
    37681 atctatgcga ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca
    37741 aaacgcggac gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt
    37801 tcgttccact gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga
    37861 agatgaaaac aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga
    37921 catctatggt cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc
    37981 ctttgatacc agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa
    38041 tttcttcact atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc
    38101 acgctgggca atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat
    38161 gaatactcca agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg
    38221 ggctggcttc catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat
    38281 gccattacta attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg
    38341 gtgaaagaag cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac
    38401 atgcagacga ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt
    38461 ttcctccttc tttttattct cattgttgga atctatgttg cctatacagg attggttaag
    38521 cgagaaacga cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg
    38581 gcttccttta ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat
    38641 gtcagtaccg ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa
    38701 gggaaagaca gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca
    38761 tggctattgc ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa
    38821 agtttattgt ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt
    38881 gaagaaattg aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta
    38941 ggaacagtct tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta
    39001 acaggaatca tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc
    39061 agtttcctat taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag
    39121 ttatttaatg tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt
    39181 atttccacta tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta
    39241 caaattgtga cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact
    39301 ttacaaagca acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg
    39361 atgaatcgac aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca
    39421 gggtcaacga agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat
    39481 actcgtcaaa atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag
    39541 caacaaggcg gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa
    39601 aatcgtctag tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat
    39661 gtcaaatatg cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa
    39721 caatctatgg cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa
    39781 aaacgaaaga acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga
    39841 aaagaaaacc catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa
    39901 accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa
    39961 aaagagcaaa aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga
    40021 acggataagc gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct
    40081 tctctctctt cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca
    40141 gtggcgaaaa caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag
    40201 tgttggcaca ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg
    40261 tttcttatat cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc
    40321 aaagttccga atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac
    40381 aaggggttaa atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag
    40441 attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg
    40501 ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa
    40561 aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg
    40621 gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat
    40681 ttgatgatga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct
    40741 atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg
    40801 tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc
    40861 aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata
    40921 atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg
    40981 caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg
    41041 gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag
    41101 cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt
    41161 ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt
    41221 ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt
    41281 gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa
    41341 tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat
    41401 gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt
    41461 aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg
    41521 aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac
    41581 aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg
    41641 gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa
    41701 cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta
    41761 gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt
    41821 aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc
    41881 tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa
    41941 acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag
    42001 attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt
    42061 tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact
    42121 atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata
    42181 gcgggaacaa ataattagat gtcctttttt aggagggctt agttttttgt acccagttta
    42241 agaatacctt tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtat
//
LOCUS       DACEDD010000019        41494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEDD010000019 DACEDD010000000
VERSION     DACEDD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.019"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IWO45_001171"
     CDS             complement(1273..1938)
                     /locus_tag="IWO45_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332657.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IWO45_001172"
     CDS             2064..4100
                     /locus_tag="IWO45_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0332658.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IWO45_001173"
     CDS             4104..4742
                     /locus_tag="IWO45_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332659.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IWO45_001174"
     CDS             4770..5198
                     /locus_tag="IWO45_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332660.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IWO45_001175"
     CDS             5655..6566
                     /locus_tag="IWO45_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0332661.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IWO45_001176"
     CDS             6783..7220
                     /locus_tag="IWO45_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332662.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IWO45_001177"
     CDS             7315..8199
                     /locus_tag="IWO45_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0332663.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IWO45_001178"
     CDS             8423..11590
                     /locus_tag="IWO45_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332664.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IWO45_001179"
     CDS             11610..12155
                     /locus_tag="IWO45_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0332665.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IWO45_001180"
     CDS             12183..12638
                     /locus_tag="IWO45_001180"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0332666.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IWO45_001181"
     CDS             12635..13393
                     /locus_tag="IWO45_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0332667.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            13894..15093
                     /locus_tag="IWO45_001182"
     CDS             13894..15093
                     /locus_tag="IWO45_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332668.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            15115..15327
                     /locus_tag="IWO45_001183"
     CDS             15115..15327
                     /locus_tag="IWO45_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0332669.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            15402..15878
                     /locus_tag="IWO45_001184"
     CDS             15402..15878
                     /locus_tag="IWO45_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAQ0332670.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            15875..17683
                     /locus_tag="IWO45_001185"
     CDS             15875..17683
                     /locus_tag="IWO45_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0332671.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            17760..17975
                     /locus_tag="IWO45_001186"
     CDS             17760..17975
                     /locus_tag="IWO45_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAQ0332672.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            18067..18930
                     /locus_tag="IWO45_001187"
     CDS             18067..18930
                     /locus_tag="IWO45_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332673.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            18961..19503
                     /locus_tag="IWO45_001188"
     CDS             18961..19503
                     /locus_tag="IWO45_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAQ0332674.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            19517..19939
                     /locus_tag="IWO45_001189"
     CDS             19517..19939
                     /locus_tag="IWO45_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0332675.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            19869..22268
                     /locus_tag="IWO45_001190"
     CDS             19869..22268
                     /locus_tag="IWO45_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAQ0332676.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            22300..24291
                     /locus_tag="IWO45_001191"
     CDS             22300..24291
                     /locus_tag="IWO45_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0332677.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            24314..24565
                     /locus_tag="IWO45_001192"
     CDS             24314..24565
                     /locus_tag="IWO45_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAQ0332678.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            24555..25784
                     /locus_tag="IWO45_001193"
     CDS             24555..25784
                     /locus_tag="IWO45_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAQ0332679.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            25781..27862
                     /gene="topB"
                     /locus_tag="IWO45_001194"
     CDS             25781..27862
                     /gene="topB"
                     /locus_tag="IWO45_001194"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0332680.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            28011..31931
                     /locus_tag="IWO45_001195"
     CDS             28011..31931
                     /locus_tag="IWO45_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAQ0332681.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            31932..32876
                     /locus_tag="IWO45_001196"
     CDS             31932..32876
                     /locus_tag="IWO45_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332682.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            32880..33083
                     /locus_tag="IWO45_001197"
     CDS             32880..33083
                     /locus_tag="IWO45_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332683.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(33383..33619)
                     /locus_tag="IWO45_001198"
     CDS             complement(33383..33619)
                     /locus_tag="IWO45_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332684.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(33802..34173)
                     /locus_tag="IWO45_001199"
     CDS             complement(33802..34173)
                     /locus_tag="IWO45_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332685.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(34170..35498)
                     /locus_tag="IWO45_001200"
     CDS             complement(34170..35498)
                     /locus_tag="IWO45_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0332686.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(35459..35788)
                     /locus_tag="IWO45_001201"
     CDS             complement(35459..35788)
                     /locus_tag="IWO45_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0332687.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(36046..36417)
                     /locus_tag="IWO45_001202"
     CDS             complement(36046..36417)
                     /locus_tag="IWO45_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0332688.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            36562..36747
                     /locus_tag="IWO45_001203"
     CDS             36562..36747
                     /locus_tag="IWO45_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0332689.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            37259..37477
                     /locus_tag="IWO45_001204"
     CDS             37259..37477
                     /locus_tag="IWO45_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332690.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            37690..38349
                     /gene="vanR"
                     /locus_tag="IWO45_001205"
     CDS             37690..38349
                     /gene="vanR"
                     /locus_tag="IWO45_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0332691.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            38352..39695
                     /gene="vanS"
                     /locus_tag="IWO45_001206"
     CDS             38352..39695
                     /gene="vanS"
                     /locus_tag="IWO45_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0332692.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            39866..40672
                     /gene="vanY"
                     /locus_tag="IWO45_001207"
     CDS             39866..40672
                     /gene="vanY"
                     /locus_tag="IWO45_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0332693.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            40685..41494
                     /gene="vanW"
                     /locus_tag="IWO45_001208"
                     /pseudo
     CDS             40685..41494
                     /gene="vanW"
                     /locus_tag="IWO45_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT        11616 a         9542 c        10338 g         9998 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaat acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaatttt aggttatcgc tgaaagcccc ggaaatacgg
    13681 gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg gattgagccg
    13741 catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca tcataaattg
    13801 aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag gacgccgctt
    13861 ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg ccctgaccgg
    13921 gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg cggcggggcg
    13981 gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa atatcaattc
    14041 tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc agcggtcaaa
    14101 ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc
    14161 tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa ggccaagggc
    14221 ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa aggactgtcc
    14281 tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct tgaaaaagct
    14341 ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga tatggtctat
    14401 accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac atcggataac
    14461 ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac gcaattaaat
    14521 aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga tggacaaagt
    14581 acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga ggcagcggct
    14641 acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta tcgggaaatc
    14701 attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga ccgtgaacag
    14761 cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa gacaatccgt
    14821 gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa gctgaccggc
    14881 gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt ccgcaatatc
    14941 aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa ctactacacg
    15001 gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc cggcctgccg
    15061 gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga ccctatgaag
    15121 cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt tgatctggcc
    15181 gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg
    15241 aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg ggccgacgac
    15301 ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg gtggttccgc
    15361 cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa gaagttactc
    15421 aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg
    15481 cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac cacggccagc
    15541 agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag atcaccgagg
    15601 ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt accttacgaa
    15661 aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag gatgcagata
    15721 accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag cagcggccct
    15781 ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg
    15841 ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa agaaattgct
    15901 gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta
    15961 ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg atggttttgc
    16021 ggcggctttt gccaacccgc tgccaagcct gcacctgttt gacctgtgta tcggtatcgc
    16081 cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga agaaataccg
    16141 caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag cgccttacat
    16201 cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta ccatgaccgg
    16261 caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg
    16321 ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc cgaccacgga
    16381 ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag
    16441 gatgttccag caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc
    16501 catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa
    16561 taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa
    16621 gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga
    16681 ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga
    16741 cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga
    16801 ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc
    16861 catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct
    16921 gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct
    16981 cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct
    17041 aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt
    17101 acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat
    17161 tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagtt
    17221 aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt
    17281 ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat
    17341 tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca
    17401 aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg
    17461 tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca
    17521 tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt
    17581 tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct
    17641 gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat
    17701 tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta
    17761 tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg
    17821 ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg
    17881 caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca
    17941 ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac
    18001 ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg
    18061 ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat ggacggtatc
    18121 acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc
    18181 gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct gcacagcctg
    18241 tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat gtgctatgag
    18301 cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc catgttcttc
    18361 cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc
    18421 atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg
    18481 cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt ggaggctatg
    18541 agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct caccatgaat
    18601 atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat ctatgttgtc
    18661 acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg cggcatgggc
    18721 caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat catggttgtg
    18781 gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat atccggcgca
    18841 atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt
    18901 atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca caggaggaac
    18961 ttgtttattt caacgtacaa catcatttac gctgacccgc cgtggcggta tgactgcaaa
    19021 aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga gctgtgcgcc
    19081 ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc cacattcccc
    19141 atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa gaccgtggct
    19201 tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct gggccgctgg
    19261 acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct
    19321 gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa gccggatgtg
    19381 acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct gtttgccaga
    19441 cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg
    19501 tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc atgtgaaaag
    19561 caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg cgctggtgat
    19621 cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg cggccctgct
    19681 catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc acggccagcc
    19741 ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa aggagcgtcc
    19801 ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg aacaggaggt
    19861 aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg
    19921 cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc aggccccaca
    19981 cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc
    20041 ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt gcccagccgg
    20101 acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg gacgcctcca
    20161 ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac gccaagagct
    20221 ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac accggcattc
    20281 tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac ctgacattca
    20341 ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc ggctttgacc
    20401 tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc tgggaacggc
    20461 tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt gactggaagt
    20521 ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc ctgtgcttcg
    20581 gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc
    20641 tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa agcggtgttt
    20701 tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc atcaagcgca
    20761 aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc cgcagcggtt
    20821 acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc aagaagctgc
    20881 tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt gtcctcaacg
    20941 tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc gtggcccaaa
    21001 agcataactg tccccttgtc cggctggact accagcagga acagggcctt gcaagcagcc
    21061 tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc agcgtggccg
    21121 tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg tattacggca
    21181 tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt tgccccaatg
    21241 ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc gagatcgtca
    21301 gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc gaatattacc
    21361 cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc aaggactttg
    21421 tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg gccctgaaat
    21481 ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg ctggaagcca
    21541 ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat ctggcagacc
    21601 cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta gatcagcatg
    21661 tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg
    21721 tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt gacattaagg
    21781 agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag atatgggggc
    21841 gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac gagttccatt
    21901 tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag cggtttagga
    21961 aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt tcctccccgg
    22021 aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag gcgtccgggg
    22081 atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag tacatcgaca
    22141 attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc
    22201 ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg
    22261 gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct gaaagccaag
    22321 gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca ggccaccggc
    22381 gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc
    22441 ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca taaggccaag
    22501 aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct gcaccggcca
    22561 tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca gccctatatc
    22621 cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat ccccaagaag
    22681 cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg
    22741 cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc aggccgtcct
    22801 gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca tgaaaacgtg
    22861 ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg aaaagccatc
    22921 cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa ggccgagcgc
    22981 aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga taacccggag
    23041 atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca gatcaagcgc
    23101 cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc acagggcgcg
    23161 gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg
    23221 gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt gttggggctt
    23281 ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg cagcgccgga
    23341 agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc cgccgaggac
    23401 gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga ggccacccat
    23461 gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta tgtgctgatt
    23521 tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca gggtgatttg
    23581 tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga aacccgttac
    23641 cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga cacctaccaa
    23701 gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct gtcccatgtg
    23761 ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat gtccacgcta
    23821 ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac cctgacgccg
    23881 ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc cgccatgctc
    23941 aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc tcccagcacc
    24001 agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg gaacgtggga
    24061 cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc gcaggccaag
    24121 cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc
    24181 ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta cacgaacctt
    24241 acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta acgccagaaa
    24301 ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa
    24361 gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga aaaccgaaca
    24421 ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg aggaatttgc
    24481 cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt accatgaaca
    24541 ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc cccgcacttc
    24601 tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc
    24661 cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt ttggagcctg
    24721 aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa aaagataccg
    24781 gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt tccaaggtga
    24841 gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa aaagaacctg
    24901 aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc
    24961 ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta gtcggcacag
    25021 acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat tccgactata
    25081 tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc ctctccattg
    25141 acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc agcgccgccc
    25201 tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg aaaaatggca
    25261 agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt attgaccggg
    25321 acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc gatctgctga
    25381 cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt accacgaaat
    25441 gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg accgagtgca
    25501 gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag gaagaaccca
    25561 agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg
    25621 ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc gacgatttgg
    25681 gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa accgagtttg
    25741 acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata aacttgtaat
    25801 cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca cggagaaaca
    25861 cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac atctggtgtc
    25921 ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc
    25981 cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc agtttgacgt
    26041 tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg
    26101 gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt
    26161 tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct ttgcccacct
    26221 gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg ccaaggccga
    26281 ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca agaacctgac
    26341 tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg ccaagattac
    26401 cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg cggacgcggt
    26461 aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc
    26521 gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc ccaagctcta
    26581 tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg ccaagcagac
    26641 ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga gaacggatag
    26701 tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga
    26761 gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca tcctgaacag
    26821 caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga agcagggctt
    26881 tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca agctcttatg
    26941 tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct gtgccgggca
    27001 ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga ttgaacgccg
    27061 gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc
    27121 ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg agcatttcac
    27181 cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac gggccggggc
    27241 cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca cccgcgccgc
    27301 cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc agctcattcc
    27361 caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt ctccccagct
    27421 tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg atcctgacga
    27481 atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt cctgtatcag
    27541 tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt gtccccgctg
    27601 tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt
    27661 cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca ccccgaagat
    27721 cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac
    27781 cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt acgtcaacta
    27841 ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag tgtggctaca
    27901 ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc ttgttgggga
    27961 agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag
    28021 tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc
    28081 tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt ggcattactg
    28141 ccgacaatcc gcaggatttt tttatcaacg ggtattcctg cccggaggac agacaccttg
    28201 ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc cttgccgccc
    28261 ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg caaaatcccc
    28321 gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg gattactatg
    28381 tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg aaccgttccg
    28441 gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag tttggacgcc
    28501 acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg agaagcggcg
    28561 acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg gtgatgcact
    28621 acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag gcatccgaaa
    28681 gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg aacagcaaag
    28741 aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt tttgaaagta
    28801 gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt ctgaacaata
    28861 cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg tggcaggatg
    28921 aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc ccggccccgt
    28981 tcaaggttaa gcagcagatg gagaaaatcg acccggccac ccagcagccc gtgatcggcg
    29041 cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt aaggtggtga
    29101 cagtctttga catcagccag accgagggca aggattttcc catgccctat atcagtgaac
    29161 ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg acatcaccct
    29221 atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc tacgcaaaac
    29281 agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc gccatccatg
    29341 agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag gaacaggcgg
    29401 cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat accgtctgcc
    29461 agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat tggagcagcg
    29521 gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg gccagcgaat
    29581 tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag gcccggcagg
    29641 aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg caggccgagg
    29701 aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg ctatggagcg
    29761 aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg
    29821 cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg ttccgcattg
    29881 actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt ggagataggg
    29941 acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag gacgaaaact
    30001 ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac aaggcccatc
    30061 gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc cttgcccaaa
    30121 tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg gaggaaacgg
    30181 cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc aatcagggcc
    30241 agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg caggactaca
    30301 aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg accgtggagg
    30361 aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc taccagcttc
    30421 gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta caggccgcag
    30481 ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg gataaaagca
    30541 cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat tttaggggac
    30601 acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac accgcccact
    30661 atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat cccctgcgta
    30721 ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc aacaatacgc
    30781 cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg gcagttgctt
    30841 ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct gtccgctatt
    30901 atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt tctgactacc
    30961 ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg gaaattgcgg
    31021 agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc ctccttgaca
    31081 cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc gcccgcgtcc
    31141 cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg
    31201 ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt gacaaaatcc
    31261 gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc aagctggaaa
    31321 agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc
    31381 gcaagcatgg gacgctggac ggatgccccc atctgaccga aagaggcatt gaaaacctga
    31441 aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc tggcagctct
    31501 ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg acccgccaaa
    31561 aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc aaccgggtgg
    31621 ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc gacgagctga
    31681 aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca
    31741 ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc gaaaagccca
    31801 gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg gcgcagctca
    31861 acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca aagggattgg
    31921 aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg gaacagatca
    31981 tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac attatcaggc
    32041 gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc cggccctccg
    32101 ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag tacaacacat
    32161 ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg gagctgaccg
    32221 gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg cagcatttcc
    32281 ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat gagcatggca
    32341 cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta ttgggaaact
    32401 ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg ctacggacgg
    32461 agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc accgctttgc
    32521 atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa gatctgtccg
    32581 cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac tttgcggcga
    32641 ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa tccctgtgtt
    32701 ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac gagcgccggg
    32761 atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac agcaaacagg
    32821 ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag gtataaggaa
    32881 tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat gcagaccggc
    32941 gcagaacgat aaacaggctc accgccgtca tgccggttat ggatggggat atgcgggcgc
    33001 aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat gacggccaga
    33061 agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc catgtggtcg
    33121 cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc ggtattgtcg
    33181 gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct tgcagcgggc
    33241 ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga aacagatata
    33301 gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa
    33361 gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc aatgcttcca
    33421 gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc tttttgccgt
    33481 ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa tgggccaaca
    33541 ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca tagtccagca
    33601 gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta tgataccata
    33661 ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct acgcatttag
    33721 aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc atgccggaaa
    33781 agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt atcttttccg
    33841 ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc agtatttggc
    33901 ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca cttcgtcccg
    33961 gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg ccttaaacca
    34021 gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc cgtcgtccag
    34081 caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc gggccttgcg
    34141 atattgaggg taattcattc gtggaatact catagctctt gcgtccattc ctttcccggc
    34201 gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg catctgcttt
    34261 ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg cttgtactcg
    34321 gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag ggcctttgcc
    34381 gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc cttatccgct
    34441 gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat attctttatc
    34501 agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc ggcctcgtcg
    34561 ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc tatcccatgt
    34621 tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc
    34681 tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg caaagaaatc
    34741 cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc tttgatggct
    34801 tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg tccgggagcg
    34861 cggaaagaaa taaactttcc cggcttgatc tcatagccct gcgcctgcat gagccgcagg
    34921 aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct
    34981 ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc cttactctgg
    35041 ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt gcgccggata
    35101 taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc cacggcacag
    35161 aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac atagggatat
    35221 ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg ttccggcgtg
    35281 gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc cttttggtat
    35341 gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca ggcaaaggag
    35401 gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag ggcttttttc
    35461 agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc gcccccttta
    35521 tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt gcaatctgat
    35581 tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca
    35641 tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag ttcttcgtat
    35701 gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca gggacgcgca
    35761 gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca ggggtttggg
    35821 gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc cgtttttccg
    35881 ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca agacgtttcc
    35941 aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa
    36001 gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca catcctccgt
    36061 ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct cgatgatcgg
    36121 caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca gttcgctttc
    36181 ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg ctttaataag
    36241 ctgtatgaca accggcagac tgggaatggc cccgtcgttt tcgatgttgg caagatagcg
    36301 ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc
    36361 cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat tcgccatatc
    36421 gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg tatatgcacc
    36481 tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa agaattataa
    36541 tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg cgagaaatgg
    36601 ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc cggcgctgta
    36661 aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga caggcgtaca
    36721 aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg gatatggttt
    36781 tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat agatggcagg
    36841 ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat ttacacatgg
    36901 atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa acaaagacga
    36961 ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca tctggattct
    37021 ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt gagtatgggg
    37081 tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg ggatttacat
    37141 ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat atgaggaata
    37201 tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt
    37261 gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa gccataccgc
    37321 aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg tccccaacaa
    37381 gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta aaggatattc
    37441 tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc
    37501 caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa accaataata
    37561 tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta aaatccgaaa
    37621 agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc ttaaaatgga
    37681 gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat ctgtaataca
    37741 gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga tggaaatgag
    37801 gcatacacca agttttacga aaacacttat caactggtta ttcttgatat tatgctgccc
    37861 ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg
    37921 atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatct agaggcagac
    37981 gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga agccctgctg
    38041 cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact tctgccggag
    38101 gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga atttgaaatc
    38161 cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat tttgtcacgt
    38221 atatggggat atgactttga gagtgatggc agcacagtcc acactcatat caaaaatttg
    38281 cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata ccgattggag
    38341 gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat acgatcattg
    38401 tcctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt gtgtcttatt
    38461 tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg gaactgattc
    38521 agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac aataaccaat
    38581 cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca ccaaatgcca
    38641 atacatcaaa tagttttaga cccgactttc tttatgtggt acatagagat gataatattt
    38701 cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg acaattcggg
    38761 gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat atctttgcgc
    38821 ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg gcaaacctga
    38881 aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct catgatatgc
    38941 attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg
    39001 aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat gagctaaaaa
    39061 cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc ggtgactaca
    39121 aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca
    39181 tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc atagctgaac
    39241 cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc ttggcagagg
    39301 caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg tccgatccga
    39361 ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac acgccgcagg
    39421 gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt tttgttttga
    39481 acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca ttctatcgca
    39541 ttgatcaggc gcgaagcaga aaaagtgggc gaaacggttt aggacttgcc atcgtacaaa
    39601 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    39661 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttga
    39721 ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta
    39781 cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt
    39841 tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca
    39901 tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat
    39961 ttcgttcaca gtctgcacgc tgtttttggg gcggagactg gcttccgtat tggaggcaac
    40021 acagataccg cccatccctg caactcatac aggcagcagc actgacgtag tggagaattt
    40081 ggaggaaaac gctcttgcca ccgccaaaga acagggagat gaacaggaat ggagcctgat
    40141 tttagtgaac aggcagaacc ccatccccgc acagtacgat gtggagcttg agcaactatc
    40201 aaatggtgag cggatagata ttcggatttc tccctatctt caagatttgt ttgatgccgc
    40261 aagaactgat ggagtttacc cgattgtcgc atccggatac cgaacaacag aaaaacagca
    40321 agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct ctgcacaggc
    40381 taaagcggaa gcagaaactt gggtggccgt gccgggaacg agcgagcatc agcttggtct
    40441 tgctgtggat atcaatgcgg acggaattca ttcaacaggc aacgaggttt atagatggct
    40501 ggatgaaaac agctatcgtt ttggttttat tcgtcgctac ccaccagaca agacagagat
    40561 aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga
    40621 gatgtacaac caaggggttt gccttgagga atatttaaaa ccagaaaaat gagaaaagga
    40681 tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc
    40741 gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac
    40801 gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca
    40861 caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc
    40921 aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt
    40981 acgccatgcg gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact
    41041 caccaccatg tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct
    41101 gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc
    41161 aaacagtgac gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa
    41221 agtgcgaaac gataccgact gcacctacca aatatgagtg accttagatg atgagaaaat
    41281 catcggtcag gtgtccgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag
    41341 tatccagtat gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca
    41401 agttgcctta ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa
    41461 aatctgttat cccctcccgg aaagtgtgga tatt
//
LOCUS       DACEDD010000020        41485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEDD010000020 DACEDD010000000
VERSION     DACEDD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.020"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWO45_001209"
     CDS             214..498
                     /locus_tag="IWO45_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0332694.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWO45_001210"
     CDS             complement(779..1198)
                     /locus_tag="IWO45_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0332695.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IWO45_001211"
     CDS             complement(1200..1784)
                     /locus_tag="IWO45_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0332696.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWO45_001212"
     CDS             complement(1831..3306)
                     /locus_tag="IWO45_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0332697.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWO45_001213"
     CDS             3487..3798
                     /locus_tag="IWO45_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332698.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IWO45_001214"
     CDS             complement(3913..6249)
                     /locus_tag="IWO45_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0332699.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IWO45_001215"
     CDS             6391..7059
                     /locus_tag="IWO45_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0332700.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IWO45_001216"
     CDS             7062..8105
                     /locus_tag="IWO45_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0332701.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IWO45_001217"
     CDS             complement(8342..9223)
                     /locus_tag="IWO45_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0332702.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IWO45_001218"
     CDS             9382..10110
                     /locus_tag="IWO45_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0332703.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IWO45_001219"
     CDS             10266..10583
                     /locus_tag="IWO45_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0332704.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IWO45_001220"
     CDS             10576..11184
                     /locus_tag="IWO45_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0332705.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IWO45_001221"
     CDS             11200..12030
                     /locus_tag="IWO45_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0332706.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12656
                     /locus_tag="IWO45_001222"
     CDS             12168..12656
                     /locus_tag="IWO45_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0332707.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="IWO45_001223"
     CDS             12707..13579
                     /locus_tag="IWO45_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0332708.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="IWO45_001224"
     CDS             13755..14117
                     /locus_tag="IWO45_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0332709.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="IWO45_001225"
     CDS             14126..14878
                     /locus_tag="IWO45_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332710.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="IWO45_001226"
     CDS             14892..16694
                     /locus_tag="IWO45_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332711.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="IWO45_001227"
     CDS             16971..18011
                     /locus_tag="IWO45_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0332712.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="IWO45_001228"
     CDS             complement(18063..20207)
                     /locus_tag="IWO45_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0332713.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="IWO45_001229"
     CDS             complement(20564..21937)
                     /locus_tag="IWO45_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0332714.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IWO45_001230"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IWO45_001230"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0332715.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IWO45_001231"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IWO45_001231"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0332716.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23386..24399)
                     /locus_tag="IWO45_001232"
     CDS             complement(23386..24399)
                     /locus_tag="IWO45_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0332717.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24480..25082)
                     /locus_tag="IWO45_001233"
     CDS             complement(24480..25082)
                     /locus_tag="IWO45_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0332718.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25267..25968
                     /locus_tag="IWO45_001234"
     CDS             25267..25968
                     /locus_tag="IWO45_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332719.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25978..28689
                     /locus_tag="IWO45_001235"
     CDS             25978..28689
                     /locus_tag="IWO45_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0332720.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29086..30714
                     /gene="treC"
                     /locus_tag="IWO45_001236"
     CDS             29086..30714
                     /gene="treC"
                     /locus_tag="IWO45_001236"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0332721.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30774..31955)
                     /locus_tag="IWO45_001237"
     CDS             complement(30774..31955)
                     /locus_tag="IWO45_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0332722.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32207..33733
                     /locus_tag="IWO45_001238"
     CDS             32207..33733
                     /locus_tag="IWO45_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0332723.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFAPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            complement(33802..34530)
                     /locus_tag="IWO45_001239"
     CDS             complement(33802..34530)
                     /locus_tag="IWO45_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0332724.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34543..35823)
                     /locus_tag="IWO45_001240"
     CDS             complement(34543..35823)
                     /locus_tag="IWO45_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0332725.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYAQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(35964..36566)
                     /locus_tag="IWO45_001241"
     CDS             complement(35964..36566)
                     /locus_tag="IWO45_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0332726.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36812..37018
                     /locus_tag="IWO45_001242"
     CDS             36812..37018
                     /locus_tag="IWO45_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332727.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37331..38050
                     /locus_tag="IWO45_001243"
     CDS             37331..38050
                     /locus_tag="IWO45_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0332728.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38131..38814)
                     /locus_tag="IWO45_001244"
     CDS             complement(38131..38814)
                     /locus_tag="IWO45_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0332729.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39046..41232
                     /locus_tag="IWO45_001245"
     CDS             39046..41232
                     /locus_tag="IWO45_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0332730.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13473 a         7349 c         8313 g        12350 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccatat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagttg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatgc tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgttttttca agtagttcag tagggatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cacctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttgat tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcattgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaaat tattttttaa aacgttttag
    23341 taaaacgttt taatagttcg ataaaaatat agcacacctt gaaagctatt tcaagggtgc
    23401 agttgaaaat cttttttcta atcgtacagg taatcttttt tcttcccatt ctttttctgg
    23461 gaattggatc cgatctaaca ataatttggc agatgtttgt cccaattcga agatcggttg
    23521 tgcgatcgtt gtcagtttcg gcttgatata ctcgcacata tctatattat catagccaat
    23581 aatgctgtag tcttcaggaa tgctttttcc agattcctct aatccgcgat ataatccaaa
    23641 agctaattca tcatttaaag caaacacacc agttgcagca gaatcaagaa gttctgctgt
    23701 gatttggtag ccaccttgtt ttgagaactg tgtaggtaag aggatcagtt ggtcgtgaga
    23761 gatttggtaa acatcaagag cagatttaaa cccctcaatc cgagcgtgga cattttccgg
    23821 cggattttca ggatagacaa gggcgatcgt ttgatgacct agactaagca agtgcatgcc
    23881 cgctaggtaa ccaccgcgaa aatcgtctgt tcgtacggca tcactaaatc cttcagattt
    23941 tttctgatcc aaaacaatga agggccggcc ttgttttttc aaattttcgt tgattgcatc
    24001 tgtagagaca gcagaagtcg cgataatgaa tccatccaca cctcgacgtg tgagctcggc
    24061 tagatattcg atttcttttt gatgatcaga atcagcatta cataatatcg tgacgaaatt
    24121 ctgcttatac aagatatcct caattcctcg catcagtgta ctgaagaatg gattcgtgat
    24181 atcaggaacg agtacaccaa ttgttttcgt ttccttggta atcatttgtc tagcaaaata
    24241 gtcaggctga tagcctaatt cttctttggc agctaaaact ttctgaacag ttttcggact
    24301 gaatttctcc gtatttccat ttaatatcaa ggagacagtt gtgattgaaa cgccggaaag
    24361 tttagcgact tctttgattg tgatcttttt tttcaccatg ttcatactcc tatctaaatc
    24421 ctgtggttat tttcccatga aatcaaaaag aagtaaatga aaatcacgtg tgccagtact
    24481 tatttgcttt gttggatagc ttcgattatt tgtttcgttg agcgtactcg tgcagttttc
    24541 ggaaatatct tttttatgaa caattgatgg gattctttgt cacgatcact gcaagcatct
    24601 tcagcaagta caacatgata cccatgttgg aaggcatcta atgcagtagc atatacaccg
    24661 tttgttgtcg ctacaccgct taaaacgata gtttcgattc cacgccggcg aagttgaaga
    24721 tcaagagaag taccaaaaaa ggcacttgga ttgtattttg tgatagtcaa catattgttt
    24781 gtatctttca aggaatcctt caacaacaag tcagtgaatt ctgccggagc agacaaagca
    24841 gatattcctc cagcatctgt ctgatgactt agataagaga aagtagtgat gtctacattc
    24901 actaaaactt tcaaggcagc ggtacgtttc aatgtttttg ctaacaaatc attgtttgtc
    24961 agaatagatt gtgcagtgtg tggagagagt gtaccgttat ttacgatccc tttttgcatg
    25021 tcgatcgcga caaaagcagt ttttgataaa tcaaaaatca tattttcctt taaatcattc
    25081 atgatgttcc ttctttcaaa taacaatgtt ttttatgtaa gtaaatcata aatcgtttat
    25141 ttgtcaacta aataaaggat tcctttcttt agtctagaga aattcagtgg taaaataaat
    25201 aagtatgaga aaacgcattc gatgcataga tatagcgaga aatatcgtga aaatggagga
    25261 atcagagtga agtatgtcga agtaaaaaat gaatacaaac gatacaaaat gggagaaacg
    25321 accattacag caaatgacga tatttctttt tcaattgaaa aaggcgaatt agtgattata
    25381 cttggtccaa gtggagcagg aaagtcaacg attcttaata ttttaggtgg gatggataca
    25441 cctgatgaag gtcagatcat tattgatgac acagatattg cgcaattttc agataaacag
    25501 cttactgctt accgacgaac agatgtaggg ttcgttttcc aattctacaa tctcgttcct
    25561 aatctaacag caaaagaaaa tgtggaattg gcaacggaag tttctcctga tgcgcttgat
    25621 cctgtagagg tattgaggca agtgggccta gctcatcgat tgaataactt tccgtcccaa
    25681 ttatcaggag gcgaacagca gagggtttca attgctcgtg cactagctaa aaatcccaaa
    25741 cttcttttat gtgatgaacc gacaggcgcg ctggattttg aaacgggcaa gcaagtattg
    25801 aaattattgc aaaacgctag cagacaacac ggcaataccg ttttgatcat tacacataat
    25861 tctgctttag cgcccatcgc tgatagagtc attcacatta atgatgctaa agtaaggtct
    25921 gttgaattaa atgatcatcc ttcatccatt gatgagattg tatggtaggg ggattttatg
    25981 aaaacaagaa catatcttaa agcaggtttt cgcgaaattc ggcaatctaa aggacgattc
    26041 attgcaatca tccttattgt catgctagga acacttttat ttgtaggtgt caaaactgct
    26101 ggacctgtca tgcaaaaaac gatggaccac tatgttggta cagcaggact ttctgatttg
    26161 caaatcgttt ctactggtgg cttgacacaa aaagatattg ctcaagcaga aaagatccca
    26221 gatgcaaaag tagaaacagg aaaacagatc tattattcca atactggtaa aaacgaagtg
    26281 attcaaatat tttcttataa caaaaataac aagcagaaca agctacaatt aactgatgga
    26341 aaactcccgg aaaaaccaaa ccagcttgta ttggatgaaa aagcggaaga tgaagggtat
    26401 aaaatcggtg atatttatca gattgattca gatgaattga aagaaaaaga gtataccatc
    26461 acgggctttg tccgttctcc attgttcatc aacaatttgg agagaggata cgcgaacgtc
    26521 ggaaatggta gcgtcgatta ttttgtctat ttacctgaat ccgtttttaa aacagatatt
    26581 tattctgtct tatatcttga ttttacaaac gtaaagaatt tgaatacgta cagcaaagct
    26641 tacaaagata aaatggaaca caatcaagag aaggcagaaa agtatttaca agatcgccct
    26701 gaagaacgct tagaagagct gaaaaaatcg gcaaacgaag aattagaacc agcacagcaa
    26761 aaaataacag aaggcaaagc acaaatagca cagggaagaa cacagttaga agatgctaaa
    26821 aaacaattag cgcaacagtc agcagctatc cagcagcttc ctgaagcgca acgaacaatc
    26881 gctgttcaaa cattatctca acaagaagct caacttgctg aacaagaaag gcaattagtt
    26941 gagaaagaac gtgaactggc ctctgcagag gaagaattga atcgaaatgc agaaaaaatt
    27001 gaaaatttaa ctaaaccatc atacctatac aatgaacgtt cagaaaatgt cggttttcaa
    27061 gaatttggtg atctgtctga tcgtatcgct gcgattgcca atgtttttcc tgttttcttt
    27121 ttctttattg ccgcattgat cacctttacg acaatgacac gtatggtaga agaaaaccgc
    27181 agggaaatcg ggacattaaa ggcgttaggt tatacgaaag ttgaaatcgc aaaaaaatat
    27241 gcaatctatg caagtctagc ctcagggata ggcattgttt taggaacgat attaggaacg
    27301 aatcttttgc caaggatcat ttttgagctg tctaacgagc ggtatgatat tgggagtgcg
    27361 cttatttttt atgactggcc gccgatttta caagcagctg ccgcattctt cattgccgca
    27421 tttggcgctg caatgatcgt tttattcaaa gatttaagag aacggcctgc tgcattgctt
    27481 caaccgaaag caccgaagcc agggaaacgc atttttcttg aatacatcac tccgctgtgg
    27541 tcacgcctta gctttaatca aaaaatcagt taccgaaatc tcttccgata taagtcaaga
    27601 atgcttatgg cgattatcgg aattgctgga tgtgccgggc tgatggttgc aggcgtggga
    27661 ttgaaagatt ctctaagttc tgtctcagct aaacaatttg gaccgatcat tgactatcaa
    27721 gcaatcgtta cattggattc atccacggaa gatagtcgtc aaaaagcagc ggatatgctg
    27781 cagaagcaag aaaaagtaaa tgagatccta gctattcata ctcaaaccgt agaattgaga
    27841 aaaaaaggac atgcttcgca aagtctgaca gtgatcgtac caagtaaaac agctgatttg
    27901 gatcgtttta tccatttaac tgatcttgaa gagaacccac gctccttgcc taaaacagga
    27961 atcgctatta cgcaaaaagc agcagaacta tttgacttat ctacaggaga tcatatttcg
    28021 ctttatgatg aagatcaaaa agaatggaaa gtcgaagtaa aagaaatcgt gaaaaactat
    28081 ctaggtagct ttatttatat gtcccctgaa tattatgctt cagttactga tcaatcgatg
    28141 atggataatg catttttgct gcgtacagat aagatgacaa caaaagaaga agaaatgctt
    28201 tccgagaaac tgcttgccac agacgctgtg acaaatacgt ctttcatgtc caaacaaatc
    28261 gaaacgcaag aagaatcaat ggcgaactta gactcggttg tcttaatctt tgtcgtatta
    28321 tcaggactgc tggcttttat cgtcttgtat aatctaacta atatcaatat ttccgagcgt
    28381 gtacgcgaat tatctacaat aaaagtcctt ggattttttg ataaagaagt cacgatgtac
    28441 attgttcgag aaaatatcat ctttactctt ttaggaatca tcggaggatt tggtgtagga
    28501 tatatattga ctgacttcat cttgcaacaa gcgtctatgg agaatgtcat ctttccatta
    28561 gtgatcacat ggtggtctta tgtcttatca gcaggattaa ctatcgtatt tacggttatt
    28621 gtgatgattg ttacccattt caaattaaag catatcgata tgattgatgc gttgaaatcg
    28681 aatgaataaa aacagaggtt gtgaaatcag cgttttgagc taataacgag ggtcagctgc
    28741 taattgaaca ccgtgaatca gaggttgtga aaaagctgtc ctgcatcgag caaaaggaat
    28801 actcaaataa aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa
    28861 agcttttgag caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat
    28921 agacaaatac atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata
    28981 aagataaatt ttacaaacgc tttctgttca gttataataa taaatgataa cttgtatata
    29041 taagttgcgg taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac
    29101 aaagtcattt accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga
    29161 gatttaaaag gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt
    29221 tggctaaacc ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac
    29281 acagccatcg atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca
    29341 aagaaatacc ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat
    29401 gaatggttca aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga
    29461 gaagagccaa cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat
    29521 acggggaaat attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac
    29581 caagcagtaa gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa
    29641 ggattccgtt tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataacaaa
    29701 gaaaatgatg gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa
    29761 ttgaaccaag caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct
    29821 acgacgatcg aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc
    29881 tttaatttcc atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca
    29941 tttgattttg cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt
    30001 aacggctgga atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt
    30061 gtagctatcg atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg
    30121 aaccgtggga cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat
    30181 tcatcgattt ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa
    30241 caaggattca cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct
    30301 cgaacaccca tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg
    30361 ttaaaagttg gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat
    30421 tctattttgt cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca
    30481 caaggagatt atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa
    30541 tttgaaggac agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcacc
    30601 atcccagaag gattcacaga agccaatatt cttatcgata attattccga tactgttctt
    30661 catcaaagtc tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa
    30721 aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta
    30781 aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta
    30841 ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag
    30901 ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg
    30961 attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag
    31021 tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta
    31081 gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga
    31141 atgatacata acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt
    31201 aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga
    31261 aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct
    31321 ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa aagaaaaaaa
    31381 gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg
    31441 ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc
    31501 acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctctaaa
    31561 aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc
    31621 tttgcctgtt cttcaagaaa aggaacaagc catacgcttc caagccccaa taaaaataaa
    31681 ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg
    31741 atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta
    31801 gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc
    31861 agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg
    31921 atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact
    31981 tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg
    32041 tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt
    32101 aaccatatga taaattaaat cctgttgcta aaacgtaact attacgtttt gtaaaaggcg
    32161 aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat
    32221 aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcggcg gggcaagtgg
    32281 gaaagccgag gacagtaaag aaaaattgag cgtgatgacg accttttatc ctatgtatga
    32341 ttttacaaaa gcaatcgtcg gggatgaagg agaagtagag ttattgattc cagcagggac
    32401 tgattcccac gattatgaac cttctgctaa agacatggca aagatccaag atacagatat
    32461 ctttgtttat aatgatgaaa acatggaaac atgggtcccc gcaatccaaa aaacattaca
    32521 agaaggcaat gtccatacga taaaagcgac agaaggtatg cttttacttc cgggaagtga
    32581 agaaggacat gatcatgacc atgaacacgg ggaagaaggc catacacatg agctagaccc
    32641 acatgtatgg ctagctccaa gtttagcaat caagcaggta gcaaacatac gtgatcagct
    32701 gattgaagct tacccagaaa aacaggaagt atggacaaaa aatgcagcag cctataccga
    32761 gaaacttcaa gcactccatc agctttatca agaaaccttt aagcaagcaa aacaaagatc
    32821 ttttgtcacg caacatgctg cattcaacta tctggcatta gaatatggat tgaatcaagt
    32881 atcgattgct ggtctttctt ctagtgaaga accgtctgcg gcgagaattg ctgaactgaa
    32941 acactttgtt aaagaacatg gaatcaacta tatttatttt gaagaaaacg ccaaagatag
    33001 tattgccaga acgttagcaa acgaagcagg tgtatcttta gaagtattga atcctttgga
    33061 aggattgacg aatgaacaaa tagaaaatgg agaaaactat ctttctatca tggaagcaaa
    33121 cctcgaagcg ttgaaaaaaa cgacagagac agaaaacccg ctagaagata ctttgacccc
    33181 caaaaaagaa aagagcgtct acaacggtta ttttgaagac gaggatgtac aagatcgctc
    33241 tctttcagat tgggcaggaa cttggcaaac agtagatacg tatgtagaag atgggacttt
    33301 tgctcctgtt tttgaatata aagaaaaatt gaatcaggat aagacagcac aagaatataa
    33361 agaatattat acgaagggct atcaaacaga tattgcttca atcaagatca gtgagaaaga
    33421 gatggcattt acgtttaagg atggaacaac gaaacgttca aattatcgtt atgtagggaa
    33481 agaaatcttg acctacgagg caggaaaccg tggcgtaagg tatctattcg aagcagaaga
    33541 taaggaaagt ggcgctttta gatatattca attccgtgat cacacgattg caccggctga
    33601 ctccgaacat tttcatttat atgcaggaaa tgaaagtcaa gaagcattgc tgaaagaaat
    33661 ggagaattgg ccaacatttt atccagatac aatggatgga cgtgaaattg cacaggaaat
    33721 gcttttgcac tgataggcaa aaaagaaccg aagggtttgg atcggctagt catttagtcg
    33781 gtccaaacta ttcggttctt tttatgaaat caatttcttt aatccacgga tagaggctgg
    33841 gatggtctcg ccgtttgcca acgtgacttc tctggttttt ttattaatcg accgcacatt
    33901 ttgtgtattg ataacatagg atttatgaca acggataaaa ttccgatgca ttttctcgat
    33961 ttctttgatc ttctcataga attgtaattg ggcatgagtc aaatggactt ccagtttatg
    34021 tggtgctcct agcgaagtag aaaaatattg gatgtctttg atcggtaaaa atttgatctc
    34081 atcattaaac tctattttga agatatcttt ttggatctgg gacatttgaa aatcttcgaa
    34141 aattttgctc aggatatgct cgacattttc ttttaaatca taaagatctt ctttgacgat
    34201 ataatcagtc ggttcgatgt tgctttttaa gattttcatc gacatttccg tatgggaagt
    34261 cacatagatt atttttccta agggatctaa ttcacggatc aatttaccga tcgctacacc
    34321 atccatttca ctgtcttcta gttcgatatc taaaaagtaa ataccaaaag aagaagttct
    34381 gatcttggct gcattgagaa gttcttccgg atcagaggta gaaatatgga cataactgtc
    34441 taagttttca atcatgattc tatttttgat caccgttgat aaaacttcta attgttttgg
    34501 atcgtcttca caaatataaa tgggaatcat atcattactc ctttatttta tttgtatata
    34561 aatcgtttga gaaaaacgct ggttttcaat tgatgtttca aggaaaagat aaggattggt
    34621 ctttgtcaat tcatccaaga taaacaagcc aaggccttcg ttttttggat ttgtttttgt
    34681 tgagaaccct ttttgtttca actggtttaa cggcaatgta tttgttgttg tattgatcac
    34741 acgaatcatc acggaatgat tcttcttgag tatggtcaat tcgatttttt tcttctttgc
    34801 agcactagct ccttcaatcg cattgtccaa gataatacca agcatacgta caaggtttac
    34861 cgtatgtttc gtatccagtt cgattggctc aggaaaatga agctggacga ccaacccttt
    34921 ttcctgtgcc atcatgagtt ttaatgacag taaactacgg atctcaggaa ctttcatatt
    34981 atccaacata cttagattgg ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt
    35041 ttgagcataa taagaccgta atccttctaa gtcgccttct gaaatatagc tgtgaagaga
    35101 aaaaagcaga ttttggtaat catgtttgaa ttctctcaag tcttgatagt ttttttctag
    35161 atctttgata taatcctgtt gcatcctgac ttgttgctct aataactgat cagctaattt
    35221 cttattagag attttccaat aataaaccaa gatgatcagg ccaacacaca gatatagaat
    35281 gaaaaaactc gtattcaaag ctaagacatc aggtgtttca ccagaaaaac gggtataaaa
    35341 aataatgata tagtatgtaa ataatatcaa tgtcccgatc gtaccgatca tacggcggtc
    35401 cacagtgtga aaccaagtct ctaacgattt tttaaaacaa aaagctaata aagcagataa
    35461 aaaagcggat aaggcaatgt gaaaatactg caatgaatct cctggttcaa taaccgaaga
    35521 tttcaaaaca aaaaaatcgc cgatgcttgt taagtgatct cctaaaagcg acgtcaataa
    35581 cccaaaagga agcgcgtaaa ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag
    35641 catcagcatt cctatagcaa ttagagcagt aaaaatccct aaaatcgaat agacccatcc
    35701 aatcaaaata caagaaatca gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg
    35761 gtcaatcaat aagatacaaa ttgtgtaaat caaatattgc aacgctatgg accataaaaa
    35821 cataaagtac agctcccatt gatttttacc aaattatagc acaattagcg tattattttt
    35881 ttaatttttc agggattttg aattcagaac tcataccaaa gcagcagcca gatacaaaaa
    35941 agtcagataa gttagttaat tgtttaatga ttgttttttt catgaaagat tcctccaaaa
    36001 ttcatcaagt agtttgtaat aggaagcaac gtagctattt ctaaaacaat cccaagtagc
    36061 aaataggtag aaaagtaaga agctgagaaa aataaagtaa tgagagaaaa aattagaaac
    36121 atgctgatcg ccttctttaa taatggggga cgtttctcgg gttcaatggg attgattgct
    36181 gtacctcttg atccatagcg aagcagtaac aaaaagcagc tgattaacaa aaagaccatc
    36241 attgggaagg agatagtcaa ttgcagatgt ttcatcacca agggaacgcc ccataaataa
    36301 agtagtgtcc acagcagaca aacgaattca gagtcggaat gcaatccata agcatatctg
    36361 cggatgcata tataagaaag aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga
    36421 gaaaagacta gaacgaccgc tagttttcca agattattca ataaaacttc taaggcaaat
    36481 tttacttgga catacaccat atcttcttcc gatttgtctc cgctgaataa ggcttctaat
    36541 aatttgttca caactttatc tttcatctca tacgtacctc taccacaaat tttatgctaa
    36601 aaaaaataaa aaacgtcttt tgtttcataa aaagtggttt tgacatcaaa accgtcatga
    36661 tttcctaaaa aaatcttcgt tttccaagaa ccttaccaaa aaaagtgaca gttttgatac
    36721 gaaaagtact ttttatgaca agtttgtgct ttttcgagtg atttatgtta aaaaaattac
    36781 tgaccttata aaaggtgatg gagggattct tatggaaaat gaacaattgt ttgaagtcgg
    36841 cgaaacatat gcatttaaat ctgatttatt tgaatcacca ataaaaggaa aaatagaaaa
    36901 aatctatgaa aatagtgtga tgatacgtgt catgaaatgc gcagtagaag acaatgatac
    36961 cgctcataat ctgcactggc agttagttgt acgcaaatca agtacaatag aagaataaaa
    37021 acagataaaa tgttgagagc ataaagaggt tgtgagtctc cgtttagctc tgagttacaa
    37081 ggaataatgt taaaaaacag cttttcatgt tttatgaata ttgccacttg tgaccgaggt
    37141 gttggcagga gaatactgtt tatttgaaaa aagggctgcg acaagacttt tgtcacagcc
    37201 tttattttca tgaataggag ttacttttgg ctacttttag agaagtagct tttaaggagc
    37261 agataaagtc aaggctttta tttctataaa tgaagtagta aaatgaagaa cagattttat
    37321 tacaggaagg atgccgataa ttgtgatcat tgttgcagga acgatcggtg cagggaaaag
    37381 cacactgact gaaatgcttg cccaagattt agaaacaaaa cctttttatg agaacgtgga
    37441 agataatgaa gtattgccgc tattttattc taatccagaa aagtatactt ttttattaca
    37501 gattttcttt ttgaacaaac gctttttggc aatcaaagat gctttcagcc atgatgacaa
    37561 tgtactagac cgctccatct acgaagatag tatgctgttt catttgaatg cagatttagg
    37621 gcgtgtttct gaagtagaag taaagcagta tgaaggtctt ttagaaacga tgctgaagga
    37681 gttagaagaa atttcgccac aaaagaaacc agatctactg gtttatattc gtgtatcttt
    37741 tgaaactatg ttagcgagaa tcaaaaaaag aggccgggaa tatgaacagc tagaacaaga
    37801 tcctgaactc tattcctact ataaagaact caatcgacga tacgaagagt ggtatgaaca
    37861 gtttgatata tgtcccaaaa tagtgatcga tggggacaaa tatgatttcg tagccgatcc
    37921 ggcatgtgga gaacaaatcg tacaagaaat caagatgcgg gcaaagaaaa tgacagaaga
    37981 ggcagatgcc cagtcgatcc gctacactac aaataacccg aaagcaaagg gaatctttac
    38041 aactaaataa tataaaaact aagcaaattg ctcactcgca gatatttctc tgtgagcagc
    38101 atcttgctta gtttttttga ttcattgaga tcaaatagtg aaccgattcg tatgataaaa
    38161 ttgccataaa tgaaatgtct gagtgagggc catgatcaaa aataaaatgg aaacgatcaa
    38221 aatcgaatgg gcgccaaaca gatgatgaca gaaaacaagg acaatagctc ctgcgataaa
    38281 ggcaagaacg acacttagat agtggaaaaa tcgatcaaaa tctgcttttt ctttagaaat
    38341 acaataatca tataagttcg ttgctacccc ttttaaattt ccagttgtaa ataaattagt
    38401 gtaggcacgc ccgttgattt tatcaaaagc tacccactgg atcgatgcag taaaagcaat
    38461 caaaatcgtt actaatgcgg gatgctcatg gacgggtgtc aaactaacaa agaaaaaaat
    38521 cactgcttca taatatagaa caaacaagcg ccagaaatgt ttttcttttt ttctaaaata
    38581 atcaattaga tactttgtta gaagaatccc taagaaaaag aaaagggtag acaatacttt
    38641 tttaccaaca cttagccatt ctccgtcaaa tgcttggatg atcgatagaa tgatattgcc
    38701 agtttgagca gaggcaaaag caccatattg gatataagta taactgtcca tggctcctcc
    38761 gataaatgtc agcaaaagtc ctattgtacg cccctcgtga aaggaagtct gcattttttt
    38821 catcaccttt tatgaatatt tttgtcttcc ttatcttagc ataaacgatt gaaaaaaagg
    38881 aaaaattttc agaaaactaa aatcatatcg cttcccattt tcatttttag aaaggtctgt
    38941 tagtataaag aaaaaataga caacgaaact ttaaataacc aacttgaaat aaccaacttg
    39001 aaataaacaa attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa
    39061 attctccgat tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa
    39121 gtctttcggc tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc
    39181 ggacttattg aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa
    39241 atgatggaga aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat
    39301 gatatgaaga aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc
    39361 tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg
    39421 atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag
    39481 agaaaaaccg ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa
    39541 atttgtcgaa aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat
    39601 actgcacaaa aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata
    39661 tttgatcaat atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac
    39721 ctttcagtag aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat
    39781 gcacgacagc cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa
    39841 agaagccagc aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag
    39901 aatgaggaga tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt
    39961 caagatacta atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc
    40021 aagttgattg ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca
    40081 gcatacatca agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat
    40141 tatcgttctc gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat
    40201 gatcggatac caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat
    40261 ttgttttctg atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt
    40321 caaaaaaatg ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag
    40381 cagcttgtcc aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca
    40441 tatgaaggta tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg
    40501 caaacagcac tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg
    40561 atggcagact gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa
    40621 aatatagccg ttgcagatta tattttgtcg ataagcccca atttaaaatc aaaatctgca
    40681 gaaatccgag agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg
    40741 cttgttcaaa gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa
    40801 gaatggatac gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg
    40861 caagaaaccc aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga
    40921 aaacttgatg cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat
    40981 gtctttttga ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa
    41041 gtaatcgact ttaatgaaat aaaagcagaa ccagtgacga cgatagaaga agaacgaaga
    41101 ctattttatg ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg
    41161 aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa
    41221 aaaggaaagt gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg
    41281 aatgtacgaa aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta
    41341 tttctggaac accgtttatt cggaaaaagg aggtgtgaca agaattttgt cacacctccc
    41401 ttatttattt tgaaaatgag aatactatta tcaaaataga gaatatatag aataattctc
    41461 attattcaaa cgtaaataat cagtt
//
LOCUS       DACEDD010000021        40587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEDD010000021 DACEDD010000000
VERSION     DACEDD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.021"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            666..2276
                     /locus_tag="IWO45_001246"
     CDS             666..2276
                     /locus_tag="IWO45_001246"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0332731.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2568..3437
                     /locus_tag="IWO45_001247"
     CDS             2568..3437
                     /locus_tag="IWO45_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0332732.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3602..4873
                     /locus_tag="IWO45_001248"
     CDS             3602..4873
                     /locus_tag="IWO45_001248"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0332733.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4870..6165
                     /locus_tag="IWO45_001249"
     CDS             4870..6165
                     /locus_tag="IWO45_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0332734.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6264..6527
                     /locus_tag="IWO45_001250"
     CDS             6264..6527
                     /locus_tag="IWO45_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0332735.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6972..7421
                     /locus_tag="IWO45_001251"
     CDS             6972..7421
                     /locus_tag="IWO45_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0332736.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7478..8896
                     /locus_tag="IWO45_001252"
     CDS             7478..8896
                     /locus_tag="IWO45_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0332737.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8896..9756
                     /locus_tag="IWO45_001253"
     CDS             8896..9756
                     /locus_tag="IWO45_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0332738.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9930..10703
                     /locus_tag="IWO45_001254"
     CDS             9930..10703
                     /locus_tag="IWO45_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0332739.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10850..11215
                     /locus_tag="IWO45_001255"
     CDS             10850..11215
                     /locus_tag="IWO45_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0332740.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11363..12571)
                     /locus_tag="IWO45_001256"
     CDS             complement(11363..12571)
                     /locus_tag="IWO45_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0332741.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12590..12868)
                     /locus_tag="IWO45_001257"
     CDS             complement(12590..12868)
                     /locus_tag="IWO45_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332742.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13061..13717
                     /locus_tag="IWO45_001258"
     CDS             13061..13717
                     /locus_tag="IWO45_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0332743.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(14159..14956)
                     /locus_tag="IWO45_001259"
     CDS             complement(14159..14956)
                     /locus_tag="IWO45_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0332744.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14953..16137)
                     /locus_tag="IWO45_001260"
     CDS             complement(14953..16137)
                     /locus_tag="IWO45_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0332745.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16162..17409)
                     /locus_tag="IWO45_001261"
     CDS             complement(16162..17409)
                     /locus_tag="IWO45_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0332746.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17624..19369
                     /gene="dnaX"
                     /locus_tag="IWO45_001262"
     CDS             17624..19369
                     /gene="dnaX"
                     /locus_tag="IWO45_001262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0332747.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19389..19703
                     /locus_tag="IWO45_001263"
     CDS             19389..19703
                     /locus_tag="IWO45_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0332748.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19728..20324
                     /gene="recR"
                     /locus_tag="IWO45_001264"
     CDS             19728..20324
                     /gene="recR"
                     /locus_tag="IWO45_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0332749.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20388..21032
                     /locus_tag="IWO45_001265"
     CDS             20388..21032
                     /locus_tag="IWO45_001265"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0332750.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21047..21376
                     /locus_tag="IWO45_001266"
     CDS             21047..21376
                     /locus_tag="IWO45_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332751.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21390..22328
                     /gene="holB"
                     /locus_tag="IWO45_001267"
     CDS             21390..22328
                     /gene="holB"
                     /locus_tag="IWO45_001267"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0332752.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22364..23188
                     /locus_tag="IWO45_001268"
     CDS             22364..23188
                     /locus_tag="IWO45_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0332753.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23181..23528
                     /locus_tag="IWO45_001269"
     CDS             23181..23528
                     /locus_tag="IWO45_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0332754.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23597..24469
                     /gene="rsmI"
                     /locus_tag="IWO45_001270"
     CDS             23597..24469
                     /gene="rsmI"
                     /locus_tag="IWO45_001270"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0332755.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24578..25699
                     /gene="dinB"
                     /locus_tag="IWO45_001271"
     CDS             24578..25699
                     /gene="dinB"
                     /locus_tag="IWO45_001271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0332756.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="IWO45_001272"
     CDS             complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="IWO45_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0332757.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="IWO45_001273"
     CDS             complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="IWO45_001273"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0332758.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29185..29952
                     /locus_tag="IWO45_001274"
     CDS             29185..29952
                     /locus_tag="IWO45_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332759.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29942..31984
                     /locus_tag="IWO45_001275"
     CDS             29942..31984
                     /locus_tag="IWO45_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332760.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32265..32414
                     /locus_tag="IWO45_001276"
     CDS             32265..32414
                     /locus_tag="IWO45_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0332761.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32425..33939
                     /gene="dltA"
                     /locus_tag="IWO45_001277"
     CDS             32425..33939
                     /gene="dltA"
                     /locus_tag="IWO45_001277"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0332762.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33936..35141
                     /gene="dltB"
                     /locus_tag="IWO45_001278"
     CDS             33936..35141
                     /gene="dltB"
                     /locus_tag="IWO45_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0332763.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35186..35419
                     /gene="dltC"
                     /locus_tag="IWO45_001279"
     CDS             35186..35419
                     /gene="dltC"
                     /locus_tag="IWO45_001279"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0332764.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35422..36690
                     /gene="dltD"
                     /locus_tag="IWO45_001280"
     CDS             35422..36690
                     /gene="dltD"
                     /locus_tag="IWO45_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0332765.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36831..37913
                     /locus_tag="IWO45_001281"
     CDS             36831..37913
                     /locus_tag="IWO45_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0332766.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37913..38716
                     /locus_tag="IWO45_001282"
     CDS             37913..38716
                     /locus_tag="IWO45_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0332767.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38786..39526
                     /locus_tag="IWO45_001283"
     CDS             38786..39526
                     /locus_tag="IWO45_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0332768.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39537..40541
                     /locus_tag="IWO45_001284"
     CDS             39537..40541
                     /locus_tag="IWO45_001284"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0332769.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13042 a         7107 c         8458 g        11980 t
ORIGIN      
        1 ttttgtcgca tctccttatt ttttacttat gtgattcgtt accttcaatg aatccctaca
       61 aggcactttt gttgaaaaaa aggtcatctt tgaaaaaatt agtggaattg ttttgttttt
      121 gatattattt aatagaaaag atatgccaaa aatccgagaa ctgtggacta cgttttttac
      181 gtaaaggagg taaataaggg tgaaagaaaa agattgtttc tacgataagc tatataaact
      241 gttgctgcaa gtaggaaaaa gtagttcttt gttaggtggg atattatcta tcataatgtt
      301 tgttttattt ttttatctat tatttcactt aaaatttttc agtaacgatt ttctgatttg
      361 cttaggttta acgttagcta tgtttatatc aagcttatta tgtgggttaa attttttttg
      421 cactcataaa ggacttaaga aataatttct caaaggatat cgaaaaagaa aaaattgtaa
      481 gcggatagta ttcatttgag tacttgactt tcccttagtc agcttgtatt atacaccttg
      541 ggcacccttt tagagggtca gaagtttaca gaatttcggc tccctattct tattgaagaa
      601 tagggagctt tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg
      661 aacttatgac aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga
      721 ttgttgcagc ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa
      781 aatttgatcc atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag
      841 tattcgtaac agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta
      901 ttgacatcaa tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt
      961 tgcgtaaaga acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta
     1021 caaatgagat caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca
     1081 ttacagaagt agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc
     1141 gtcaaatgaa agcagatgtc ggtacagata atgttatgta tatccataca acattgattc
     1201 cttacttgaa agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc
     1261 tacgtggttt aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc
     1321 aaagcgtgaa aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt
     1381 cgccagatgt agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc
     1441 aaattgtttg cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag
     1501 aattagaaga acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat
     1561 atgtcgagtt accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg
     1621 cctataattc agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg
     1681 tagaagaacg tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg
     1741 gagtagaagg gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag
     1801 gtatttgtct tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag
     1861 aagatgcgtc atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg
     1921 ctgatcagga aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta
     1981 aaatcaaaaa aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac
     2041 gtcaccgtca ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc
     2101 tagtcttttc aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga
     2161 aaaaattctt tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc
     2221 aaaaattaat caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt
     2281 tgaacttgat agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt
     2341 ttctcttttt ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt
     2401 tcgattaaag cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt
     2461 ttcattgatt ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga
     2521 attgtcttaa caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat
     2581 caggagctga atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca
     2641 caaacaactt ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag
     2701 tacttatcca aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag
     2761 atttaatcac tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag
     2821 accatggtga ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt
     2881 tcgatggttc acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa
     2941 aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag
     3001 acggaatcat cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta
     3061 caggtatcga ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact
     3121 ggaaaggatt agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc
     3181 ctttagtatt acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa
     3241 tgggtatctc aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc
     3301 gtaaatatat cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat
     3361 tagctccagg taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt
     3421 cagctaacaa agcgtaagca tccacataac agatacgata tataggaatg gagaacgact
     3481 tttgaagtcg ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt
     3541 ttatctatga taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca
     3601 gatgaaaaaa atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg
     3661 tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat
     3721 cttggaaggt gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat
     3781 gggcgcaact gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc
     3841 gattccgatg ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac
     3901 actgcttggt aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc
     3961 tagaccgatt gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga
     4021 acatggtgcc atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat
     4081 ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg
     4141 acagacagta attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact
     4201 gaataatatg ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt
     4261 cgaaacatta catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta
     4321 tctagcatta gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca
     4381 tttggaaagc tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga
     4441 tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta
     4501 tccaggattt gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg
     4561 cacttcagaa atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc
     4621 acgaatgggc gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa
     4681 acttcacggc acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc
     4741 tggactattg gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta
     4801 tgatcatatt atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt
     4861 gggagctgaa tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa
     4921 gtttatagtt atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa
     4981 ttatctctag ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga
     5041 atattggata tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca
     5101 agtgcagaag atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg
     5161 gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg
     5221 cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca
     5281 gccttaacac ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta
     5341 tctacccgaa tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc
     5401 gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa
     5461 aactatccgg aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg
     5521 gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa
     5581 aaccatactc gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa
     5641 cgagatgtcg ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc
     5701 gttccaccaa gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct
     5761 aagaaatttt tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg
     5821 accgctttag tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga
     5881 actggaaatc tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc
     5941 atcgatatca aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta
     6001 gaagaaattt ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa
     6061 ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat
     6121 gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg
     6181 aaacatgtta agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag
     6241 cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag
     6301 tattcatgga ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag
     6361 aaacagttga atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg
     6421 actcacatcc attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc
     6481 gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat
     6541 aactcaacgt ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa
     6601 tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa
     6661 actagtatat agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga
     6721 ggggttattt cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa
     6781 ggaaggcttt ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta
     6841 atgacttata cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt
     6901 acagctagcg atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg
     6961 agaggttttt tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat
     7021 cgatagattc aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata
     7081 aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca
     7141 caggaataga agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag
     7201 cactgtgttt ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa
     7261 ttgaatatat ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc
     7321 tcagcacact ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca
     7381 ctacgccagc agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc
     7441 cgagtatcga tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc
     7501 ttgcccgact ggaattgctc atacttatat gtcggtagaa aaattagaag taacagcaag
     7561 aaatctaaac catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc
     7621 tgaacaagaa atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt
     7681 ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc
     7741 gcaagtagta atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa
     7801 agaaccagct gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa
     7861 ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg
     7921 gattacagcg gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag
     7981 gatcggagga gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac
     8041 atcgattgct ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac
     8101 aggtggttca ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga
     8161 ttttctggca aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt
     8221 cgtccccttt ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc
     8281 gatcacatgg gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg
     8341 gctattgttc ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa
     8401 taaatcgact tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc
     8461 tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt
     8521 caaacataaa tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc
     8581 ttcgtatatt acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc
     8641 tagtttgatg acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc
     8701 aatggcacct catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc
     8761 tatgtatgtc ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt
     8821 gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa
     8881 tgaggtggaa gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat
     8941 caatttgcag taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa
     9001 gaagcagaga gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat
     9061 ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc
     9121 gttatccact tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc
     9181 tattcttccg tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg
     9241 aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg
     9301 attgggaata atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat
     9361 caagcgaaga tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca
     9421 gcgcatggct tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa
     9481 ttgaaccaac aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca
     9541 gaagtcagcg aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa
     9601 agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg
     9661 aatgcaattt atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa
     9721 attttgaaca cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg
     9781 gtatgacaat agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt
     9841 cgaaaaattt cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg
     9901 ttactatata aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg
     9961 agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag
    10021 taaattatag cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga
    10081 tccgtagagt tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc
    10141 tgatgaagat caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt
    10201 cttctttgat tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact
    10261 tagtcagaaa cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag
    10321 ctgctatgtt gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc
    10381 cgaaacgagc aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg
    10441 atttagcgat aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg
    10501 aaggagaggc aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag
    10561 ttacacagca aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg
    10621 attatctggt gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc
    10681 atatcgaatg tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt
    10741 tagataggac cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta
    10801 tcttgttaga taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt
    10861 gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg
    10921 tttatacggt gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat
    10981 ctatgtgcta ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc
    11041 agatgctggt tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt
    11101 cgatatttgg atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt
    11161 ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa
    11221 cagttaaact attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag
    11281 aataatcata aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt
    11341 tttttttatc tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct
    11401 ctgcttcatg actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa
    11461 tgactccggc aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca
    11521 aataaggacc aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac
    11581 tgattcgatg ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac
    11641 cataacctag gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac
    11701 tcagtaaaaa taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc
    11761 ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata
    11821 cgacgaagaa aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa
    11881 atgataaaac acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg
    11941 ctttcttttc aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag
    12001 ataatggaat attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta
    12061 caattgaaaa ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa
    12121 atggcccaac agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt
    12181 ttttagatgc agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt
    12241 agccgaatcc attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat
    12301 atgctaatgt tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa
    12361 atagttctaa ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc
    12421 cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca
    12481 tgatgaccat caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa
    12541 taaaatattt attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat
    12601 atgcttttaa tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa
    12661 caagttggcg aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt
    12721 cgtttgttgg attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat
    12781 ttaaaaagac taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat
    12841 taatgattgt gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact
    12901 tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact
    12961 taacaaatgt taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg
    13021 tatgatcttt ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa
    13081 ggaatatttg gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac
    13141 attcgaaggg atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag
    13201 tgttatttca aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt
    13261 ctctctttta aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga
    13321 atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca
    13381 agacccaaag ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat
    13441 gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta
    13501 cgaattatat gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat
    13561 cacgaatgaa gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat
    13621 tttaaaacaa ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt
    13681 gaatatggac ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca
    13741 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    13801 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
    13861 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    13921 tattgtgact tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa
    13981 aagctgttct gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa
    14041 cttggctgga cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag
    14101 gggctgtgac acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt
    14161 agaaacttct gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc
    14221 caccgccgct tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg
    14281 tcagacgatc ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt
    14341 agatgcctga tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga
    14401 taagagccaa aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa
    14461 ttttgccagt ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg
    14521 ttgctgattt agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat
    14581 cataaacatc atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac
    14641 cagaagtcga aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt
    14701 gatcatttcc gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat
    14761 tagatgtagt cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct
    14821 gttgttccaa agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag
    14881 ctgtatctgc atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa
    14941 aaaatttttt catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa
    15001 aatcccaaga gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt
    15061 tcctgtttgt ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata
    15121 agtaacaagc cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga
    15181 agttcgcaca ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga
    15241 atagtctttt aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt
    15301 ttctgcttgg aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa
    15361 aggttgcacg ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag
    15421 ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc
    15481 gatgtcacct atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac
    15541 ttcagcatct gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt
    15601 attgaaaaaa gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat
    15661 tgcttcttct ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa
    15721 acgtcccgat agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc
    15781 atcagtcacg atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt
    15841 atctgcagat tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc
    15901 ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga
    15961 aggttcttct ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg
    16021 gctgacttct tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt
    16081 gggatcaaaa gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag
    16141 ctgcacacct ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt
    16201 tggacagaat ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg
    16261 catttgtagc ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa
    16321 ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc
    16381 agtttgttga ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc
    16441 ttgagaaaga taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat
    16501 tgctcctgct tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta
    16561 tccttcacgt acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt
    16621 cacagaatca tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg
    16681 taattcacgc caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt
    16741 agatggtaca taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag
    16801 cgctgttagg aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt
    16861 gtttttaggg attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt
    16921 gccatgtgcc cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc
    16981 gcttgtaccg aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt
    17041 ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag
    17101 tgttccattg aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc
    17161 ggtataatcg atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg
    17221 gacgactgtt ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc
    17281 atctttgcgg actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc
    17341 gatgacttct agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat
    17401 aagacccata aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta
    17461 ttataccata ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg
    17521 cgtattgttt aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac
    17581 gtataataag agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact
    17641 ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca
    17701 gacactaaaa aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc
    17761 cagaggaacc ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa
    17821 cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac
    17881 gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt
    17941 tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga
    18001 tgaggtacat atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc
    18061 aaaagaaagt gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat
    18121 catttcacgg actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca
    18181 tcttgaattt atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat
    18241 tgcccgttct gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc
    18301 gtttagtgaa ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga
    18361 cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga
    18421 aacattgaac caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt
    18481 acaatactgc cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc
    18541 gagtacgatc agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt
    18601 ttcaatgata aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc
    18661 tatttatttg gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc
    18721 agcagatgta gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga
    18781 actaagaaac gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca
    18841 gccggtacag caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg
    18901 agtatataaa gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg
    18961 ggaagacttg ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc
    19021 agttgcagct ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag
    19081 agcagcaaat gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa
    19141 agattatgcg cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa
    19201 ttatttgacg caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga
    19261 aggagaaatc gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa
    19321 agcagaggaa ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt
    19381 agataattat gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa
    19441 tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag
    19501 ccaccaatga attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa
    19561 tcaatgaaga agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg
    19621 cagtcaatga tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg
    19681 caaaaggttt gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag
    19741 aaccaattgc gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag
    19801 cgacaagact cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta
    19861 aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag
    19921 aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg
    19981 tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc
    20041 atgttctcca tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt
    20101 cttctctgtt gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg
    20161 caacaacaga gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta
    20221 tcaaagtgac tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg
    20281 aagttacttt attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt
    20341 tttttcagat acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat
    20401 ttattacaat cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc
    20461 ctcttttgaa agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc
    20521 cgattgctga agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac
    20581 gtacggaagc actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc
    20641 ctgctctggc aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt
    20701 atcaaggtgc aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta
    20761 cagaaggtac tacgccggat tttacccttt atcttgacgt agattcagac accggattaa
    20821 gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat
    20881 tccaccagcg tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc
    20941 acaaagttga tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta
    21001 ttgaacagta tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt
    21061 agcgattatc caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat
    21121 ccgtgccaca aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat
    21181 cgtgggtatt gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga
    21241 atcaagaaag caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt
    21301 cccttatcca gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg
    21361 attccatcaa ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc
    21421 aaccgatcgt gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt
    21481 atctttttga aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc
    21541 acctgttctg tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc
    21601 ggatcgaaaa gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca
    21661 aagtcgatca gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa
    21721 aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt
    21781 tgaaattctt ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag
    21841 gaagaattct ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga
    21901 aagaagcact gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt
    21961 tggctttctt aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta
    22021 atgatgctaa ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag
    22081 cctttatcta tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta
    22141 caggattatc tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg
    22201 gcgaatatgg caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc
    22261 agaaactacg cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga
    22321 ttttttagct tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg
    22381 tgtccgatat agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt
    22441 ttatagtgac aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct
    22501 tcctaaaaaa gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa
    22561 agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt
    22621 gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga
    22681 atacacattt gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt
    22741 tagagaattg gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat
    22801 tggtgtccgc gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg
    22861 ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag
    22921 tttatctttg aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca
    22981 atatgagagc gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat
    23041 tgctacacct gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag
    23101 tattcggctg actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt
    23161 tatccaagta ggtgagatga atggataaga gatcattata cgacggattg aatcaattag
    23221 aatcagagtt gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga
    23281 tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg
    23341 aattgaacca agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc
    23401 gtatgaatct tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat
    23461 caagaaggga caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg
    23521 gtcgatgacc aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt
    23581 aaggagattg tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga
    23641 tttatatctt gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg
    23701 tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt
    23761 gaatcatttt gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg
    23821 tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc
    23881 aggaatgcct tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg
    23941 gatttctgtg atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg
    24001 gctagtgcct caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa
    24061 agaaacattg aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta
    24121 tcggatcact gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt
    24181 gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact
    24241 cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt
    24301 gttcgaaatg ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga
    24361 agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa
    24421 tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag
    24481 ttgtctataa tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg
    24541 cgaacgaatg ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt
    24601 acataatgat acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt
    24661 tgaagaacga gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag
    24721 tgacactggt gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat
    24781 tcattcagca atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc
    24841 tggcgaccat cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta
    24901 tacagatatt gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa
    24961 aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag
    25021 tgtccagcta acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc
    25081 agactatcaa aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt
    25141 aaaagctttg ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca
    25201 tgagctaggg atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg
    25261 aaattttgga aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc
    25321 agtaaacgtt acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt
    25381 gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc
    25441 actgagaaga gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta
    25501 ttctactgtg accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt
    25561 ttatcaagca agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt
    25621 agggattact ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg
    25681 ggaaaatcaa gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc
    25741 ccaaagctgt ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc
    25801 cgtacatttt tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat
    25861 ccgctggttg ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg
    25921 tccgtcaaca aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag
    25981 caattcatca aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt
    26041 tcggatacgt cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag
    26101 taaagttaat ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc
    26161 aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca
    26221 ggcaaataag cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt
    26281 aaacggtttg tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg
    26341 ttttggattt ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag
    26401 ataaaatcta ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa
    26461 gcttgttctt attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg
    26521 aaaaaagact tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc
    26581 agtaataagc ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt
    26641 acttgatgca ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt
    26701 tctttatggc ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta
    26761 cgtttgacta catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct
    26821 cgttctgtcg gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta
    26881 ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc
    26941 ggattttgtt ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt
    27001 ggataatcct tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag
    27061 tggaaactgt ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct
    27121 gtatccaaac ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa
    27181 tgataaccat attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca
    27241 agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca
    27301 acttcataca atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca
    27361 ttgacagaat cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc
    27421 gcatttgctg gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg
    27481 atttctaagc ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc
    27541 gcgattcttg gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct
    27601 tgaccatttt cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa
    27661 cttccagtta attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt
    27721 gcacattcca atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca
    27781 ccttttttca aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat
    27841 ccattgattc gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct
    27901 agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt
    27961 tcatattcca aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat
    28021 tctttttggc gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag
    28081 ttcaattctg caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat
    28141 tgacttgacg ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat
    28201 tttggcgatt cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa
    28261 tcaatcaagc agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg
    28321 atatcgcctt tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat
    28381 tttccaacga tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt
    28441 gcgttttcgt tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata
    28501 tctgttgctt tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct
    28561 aattcatatt cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt
    28621 ttgacatttt cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga
    28681 attttttcat aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt
    28741 gcttcattga tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc
    28801 atctgggatg tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt
    28861 atcttcaact cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa
    28921 ctaaatatcg gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg
    28981 tagtgtatat tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc
    29041 atatttaatt ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag
    29101 tgacaaaaac ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg
    29161 aaaaatgaaa gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa
    29221 agctacggca gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa
    29281 gaaggcgagt ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat
    29341 atcttgtcca cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc
    29401 acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt
    29461 caagagttca atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca
    29521 gttgaacgga tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg
    29581 aatatagaag aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga
    29641 attgcagctg ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact
    29701 ggctctttgg actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa
    29761 aaagaaaaag caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat
    29821 agaattttat ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga
    29881 agagagttct ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg
    29941 aatgctgtat aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta
    30001 ttttttcagt ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga
    30061 tcaatcttta gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg
    30121 tggatttatc attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt
    30181 gaatagaagg caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat
    30241 atcaggtata tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat
    30301 attactcggt attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat
    30361 ggatcttact agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt
    30421 tttcatcatc cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat
    30481 gattcgaagt tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag
    30541 gcaccgatta ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag
    30601 tcattttagg gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt
    30661 cttttttcct tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta
    30721 tacatttcgg ttatttttga tcatattgag taaatggaag atttattact atggtatcaa
    30781 ttttttgata atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct
    30841 gattacagtc attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac
    30901 tattttatcc taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac
    30961 agagaaaaaa atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac
    31021 cttgcattat aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca
    31081 aactgctaat ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt
    31141 gccgaacatt tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct
    31201 ttttcggaat atttcattat atggatctag tattggactg ccagataatc aagaattgaa
    31261 gattcaacaa atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt
    31321 actgattgta gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa
    31381 tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc
    31441 gataaagtgg ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg
    31501 agaaattagc acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga
    31561 aagctctcgt ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat
    31621 cggtatcaat atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag
    31681 tattttgaca gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt
    31741 gactaagctg gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact
    31801 taccttcttt ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt
    31861 cactcagtat ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat
    31921 ccttatctat cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga
    31981 atgatatacg gaataagaca agataataaa tagaactaaa ttttgaatac catttatttg
    32041 gaataagagg ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa
    32101 gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg
    32161 tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca
    32221 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
    32281 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
    32341 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
    32401 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
    32461 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
    32521 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
    32581 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
    32641 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
    32701 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
    32761 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
    32821 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
    32881 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
    32941 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
    33001 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
    33061 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
    33121 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
    33181 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
    33241 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
    33301 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
    33361 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
    33421 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
    33481 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
    33541 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
    33601 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
    33661 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
    33721 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
    33781 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
    33841 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
    33901 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
    33961 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
    34021 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
    34081 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
    34141 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
    34201 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
    34261 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
    34321 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
    34381 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
    34441 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
    34501 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
    34561 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
    34621 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
    34681 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
    34741 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
    34801 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
    34861 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
    34921 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
    34981 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
    35041 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
    35101 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
    35161 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
    35221 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
    35281 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
    35341 ccctgtatct gaattcgagc gagaggattg gagcacaccg aataaaatca ttcaacaagc
    35401 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
    35461 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
    35521 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
    35581 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
    35641 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
    35701 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
    35761 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
    35821 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
    35881 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
    35941 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
    36001 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
    36061 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
    36121 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
    36181 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
    36241 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
    36301 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
    36361 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
    36421 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
    36481 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
    36541 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
    36601 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
    36661 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
    36721 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
    36781 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
    36841 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
    36901 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
    36961 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
    37021 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
    37081 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
    37141 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
    37201 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
    37261 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
    37321 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
    37381 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
    37441 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
    37501 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
    37561 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
    37621 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
    37681 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
    37741 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
    37801 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
    37861 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
    37921 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
    37981 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
    38041 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
    38101 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
    38161 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
    38221 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
    38281 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
    38341 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
    38401 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
    38461 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
    38521 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
    38581 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
    38641 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
    38701 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
    38761 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
    38821 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
    38881 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
    38941 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
    39001 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
    39061 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
    39121 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
    39181 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
    39241 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
    39301 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
    39361 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
    39421 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
    39481 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
    39541 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
    39601 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
    39661 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
    39721 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
    39781 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
    39841 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
    39901 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
    39961 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
    40021 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
    40081 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
    40141 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
    40201 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
    40261 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
    40321 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
    40381 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
    40441 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
    40501 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
    40561 ccacatatat ggattccgct atttttt
//
LOCUS       DACEDD010000022        39891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEDD010000022 DACEDD010000000
VERSION     DACEDD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.022"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..709)
                     /locus_tag="IWO45_001285"
     CDS             complement(17..709)
                     /locus_tag="IWO45_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0332770.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            704..970
                     /locus_tag="IWO45_001286"
     CDS             704..970
                     /locus_tag="IWO45_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0332771.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1146..1715)
                     /locus_tag="IWO45_001287"
     CDS             complement(1146..1715)
                     /locus_tag="IWO45_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0332772.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1789..2112)
                     /locus_tag="IWO45_001288"
     CDS             complement(1789..2112)
                     /locus_tag="IWO45_001288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332773.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2266..3078)
                     /locus_tag="IWO45_001289"
     CDS             complement(2266..3078)
                     /locus_tag="IWO45_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332774.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3432..4280
                     /locus_tag="IWO45_001290"
     CDS             3432..4280
                     /locus_tag="IWO45_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0332775.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPRPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4425..5365
                     /locus_tag="IWO45_001291"
                     /pseudo
     CDS             4425..5365
                     /locus_tag="IWO45_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
     gene            5355..7526
                     /locus_tag="IWO45_001292"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IWO45_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7638..9809)
                     /gene="copB"
                     /locus_tag="IWO45_001293"
     CDS             complement(7638..9809)
                     /gene="copB"
                     /locus_tag="IWO45_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0332776.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9829..12015)
                     /locus_tag="IWO45_001294"
     CDS             complement(9829..12015)
                     /locus_tag="IWO45_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0332777.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="IWO45_001295"
     CDS             complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="IWO45_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0332778.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12237..12677)
                     /locus_tag="IWO45_001296"
     CDS             complement(12237..12677)
                     /locus_tag="IWO45_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0332779.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12751..13290)
                     /locus_tag="IWO45_001297"
     CDS             complement(12751..13290)
                     /locus_tag="IWO45_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0332780.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13905..15371)
                     /locus_tag="IWO45_001298"
     CDS             complement(13905..15371)
                     /locus_tag="IWO45_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0332781.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15682..17763)
                     /gene="cadA"
                     /locus_tag="IWO45_001299"
     CDS             complement(15682..17763)
                     /gene="cadA"
                     /locus_tag="IWO45_001299"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0332782.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17905..18084)
                     /locus_tag="IWO45_001300"
                     /pseudo
     CDS             complement(17905..18084)
                     /locus_tag="IWO45_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(18288..18647)
                     /locus_tag="IWO45_001301"
     CDS             complement(18288..18647)
                     /locus_tag="IWO45_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0332783.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18677..19018)
                     /locus_tag="IWO45_001302"
     CDS             complement(18677..19018)
                     /locus_tag="IWO45_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332784.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(19015..19689)
                     /locus_tag="IWO45_001303"
     CDS             complement(19015..19689)
                     /locus_tag="IWO45_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0332785.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKSLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20741..22039)
                     /gene="asnS"
                     /locus_tag="IWO45_001304"
     CDS             complement(20741..22039)
                     /gene="asnS"
                     /locus_tag="IWO45_001304"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0332786.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22079..23269)
                     /locus_tag="IWO45_001305"
     CDS             complement(22079..23269)
                     /locus_tag="IWO45_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0332787.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23290..23817)
                     /locus_tag="IWO45_001306"
     CDS             complement(23290..23817)
                     /locus_tag="IWO45_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0332788.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGYESGDEVKTIKGI"
     gene            complement(23864..26653)
                     /locus_tag="IWO45_001307"
     CDS             complement(23864..26653)
                     /locus_tag="IWO45_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0332789.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKATLKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26800..27000)
                     /locus_tag="IWO45_001308"
     CDS             complement(26800..27000)
                     /locus_tag="IWO45_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0332790.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27452..27772)
                     /locus_tag="IWO45_001309"
     CDS             complement(27452..27772)
                     /locus_tag="IWO45_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332791.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHILHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(28275..29393)
                     /locus_tag="IWO45_001310"
     CDS             complement(28275..29393)
                     /locus_tag="IWO45_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332792.1"
                     /translation="MAKKDKELLSQLISEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FSKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            29866..31173
                     /locus_tag="IWO45_001311"
     CDS             29866..31173
                     /locus_tag="IWO45_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0332793.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKVSGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            31291..32490
                     /locus_tag="IWO45_001312"
     CDS             31291..32490
                     /locus_tag="IWO45_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332794.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRVIFSSNNYHIF
                     RAGLYACQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(32517..33785)
                     /locus_tag="IWO45_001313"
     CDS             complement(32517..33785)
                     /locus_tag="IWO45_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0332795.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DTRLSHPWLLYLLPVSGALFAYLYVYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(33948..34595)
                     /locus_tag="IWO45_001314"
     CDS             complement(33948..34595)
                     /locus_tag="IWO45_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0332796.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(35003..35506)
                     /locus_tag="IWO45_001315"
     CDS             complement(35003..35506)
                     /locus_tag="IWO45_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0332797.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(35629..36393)
                     /locus_tag="IWO45_001316"
     CDS             complement(35629..36393)
                     /locus_tag="IWO45_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0332798.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            36500..36775
                     /locus_tag="IWO45_001317"
     CDS             36500..36775
                     /locus_tag="IWO45_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0332799.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            37371..38321
                     /gene="yidC"
                     /locus_tag="IWO45_001318"
     CDS             37371..38321
                     /gene="yidC"
                     /locus_tag="IWO45_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0332800.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(38500..39474)
                     /gene="lacD"
                     /locus_tag="IWO45_001319"
     CDS             complement(38500..39474)
                     /gene="lacD"
                     /locus_tag="IWO45_001319"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0332801.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDTTIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGILCGR
                     ATWAKGVAPFVTEGEKKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(39471..39644)
                     /locus_tag="IWO45_001320"
                     /pseudo
     CDS             complement(39471..39644)
                     /locus_tag="IWO45_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
BASE COUNT        11417 a         8385 c         7059 g        13030 t
ORIGIN      
        1 aagagggggg gattttttat cgttgtggaa tatttaaata ccaacgcttg tcatgaaaat
       61 cttgtgctcc acccttagta tttccttctg gatcgtttgt cgcacgcatc atgacgtata
      121 ctttcttatt agggaaatca cgcatattga aagatacatg atagccaaca tttcctaaag
      181 tattataagc ttgatttacg tctggtctat aaatcccatc agctcttact ctagctagtt
      241 cttttccagt attgtagtcc ataatgaaaa tatactcgta tttataatta gcaatgtgcc
      301 atccagctac atgcaagttt gcgttttcga tttctccgaa ctgatcaatg tgggcgtaat
      361 ttgttccatc tgtcagcgta gaatttgcag cacctgctcg agttggatca attactggtt
      421 tatcctccga agtagttgga ttatcatccg taaaaccatg agccaaatca taggctagtt
      481 tttctttact tacacccatt tcagaaagat aaccgtaagg atctgtatga tcgccccaaa
      541 tattttgtgt tacccataaa tgcgatttga ttcctggttg gttataagga gtgtctaatg
      601 ttaatggaat accatatttc attgctgaat ctcttgccaa ttcaacgtat actttatagt
      661 tcttttcaaa agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggacttttgg
      721 ctcacgaggt tgtcctactc tacatcttgc tgatagatat gctgaaagga aaacaccaac
      781 attatgtttt gctttttcag ctagaacgag acaagcagga atacctttag aagatatcaa
      841 agatttttta ggtcataaag atgtttccac tacacaagtc tatgcccata tttctcccga
      901 agtgaaaaaa agatcaatga atcaactaga aaactatata gaagagcaaa taaaaaagca
      961 ctcaaattga gtgctttttt aagctcacta tcactaaaac tatcactaat tttatttata
     1021 agcgaacatg tttctatcac tgatttttta aatctagcga aaaaccctta tcttaaaggc
     1081 gttcgttcaa ttctttggca aggtcttcaa agcctggctt gcctagaagt gcgaacatgg
     1141 ttttcttata gctcatgtag aaaaattttc ttgatgatga ttttatccat caattgagat
     1201 tgactgtttt gctctcctat gatttcagct atgattgtcc gattttgcgg ttttcttctt
     1261 aactggtagt tgattttccc agtttccatc aactcgctaa gcatatccac gactccgcca
     1321 aatccggtct ttttcgtaat aaaacctagt gctaccattc gttcatctag attttttgct
     1381 gtaatcgtca caccagtaat gagcgagtgc tgtaatgttt gggccagctc ttccatccct
     1441 tcagcattgg atacgacgga agctgttttg ttaataggtt cttcgtttat ttcttttggt
     1501 tctttcctct ccactttcac tgtttgacga aggacatcta gttcttgcaa gagcgtttcg
     1561 tattgttctt gggtcagcat cacgccagct actttttcac gattaaatat atatacgcca
     1621 gttcccgcgt ctctcgcttg agcaaataca tccatcggcg atcgtttcac ttctgtgatc
     1681 gaagatgtgg gaacttctaa tttctttaat tccaatggat ttcctccttt actaatcgct
     1741 ttttattata gcacagactg acttgttttg atcttttttc caaaggtttt atcggttttt
     1801 gcctaaccag taattgatat cggtaagaag tctttcgata ccaaatgaag gatggtacag
     1861 atacgttttt tgatcttcgt tatggtctag tggaaaaaaa tgatcggtga tcactagatc
     1921 tgcataccga ggcggcttat ttcgagtaca tacatgtata gagatccgtt ttcgatgtct
     1981 tagcgttttg ttcagtaccc cagcaagtct tcttgcttct tggtgttcat agcgaaaaaa
     2041 aaagacgatt ttcacttcag gagggtggac atctaaatac cctcgttcct ggagtgtttt
     2101 cagtaaaagc atcccccact ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg
     2161 aaattgatat agtttatctt tggaattggg aaatttttct aatgtttcct gataataacg
     2221 aacttcttct atcagccggt catggatacc aattacgtaa cgaagtcatt cttggtaaag
     2281 tacttttgtc agctttgatt tgctttcgct ctcaaattcg ctattcagcc aaaacagcga
     2341 atcaagcttt tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt
     2401 ccaccgcttt tcagtaatct ggttacagct caagcgcaaa attgctagat aatttttttc
     2461 ttttattggc aaaatctgct cctctctaaa tagcaattca ggaaacaatt caaagaatag
     2521 tgttcgactg tctcctaaaa tctcgatttc tgccggatcc tttctttgct tcaataattg
     2581 gatgaacatc tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt
     2641 caaaaagcaa aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt
     2701 ataagaaaaa gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg
     2761 atcgtacttc tgctcgtaag gttcattcaa tagcagattt ttcagtaaac aaaacggggg
     2821 tgattggatc agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa
     2881 ctgaacggtt ttcttatgaa gaatcagctg aatgggacag tcgctttccg ccaaacgttt
     2941 cagatgccga tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg
     3001 ccagatcaaa tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa
     3061 tagtttttgc catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa
     3121 aatgggttca ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatc
     3181 ttcctcatta ttttttattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac
     3241 tttaaaaaaa cataataaga tcactttgct tgtaggatgt attcccttcc attacaatga
     3301 aataaatagt gattttatga gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca
     3361 aacaaaaaat cgatcagttg atataagaat tagaaaaagc ggtcatcggc aaacgttcaa
     3421 ctatccaatt gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc
     3481 ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca
     3541 gcagagtcca atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca
     3601 attcacaatc aaaagagttt gaatttcgtc cgagaccgat ctttaccacg atcttgttgg
     3661 cagatgagat caatcgtacg actcctcgta ctcagtcagc acttttagaa gcaatggccg
     3721 agaatcatgc aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag
     3781 ctacgcaaaa tccaatcgag tacgaaggaa catatccttt gccggaagca cagctcgatc
     3841 gttttttatt ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt
     3901 tggataaatt cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag
     3961 aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct
     4021 tgcagttagt gtcagcaaca agaagtcatg ctgccatcca gcttggcatc agtccacgag
     4081 gaagcctttc ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg
     4141 tcacgcccaa agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt
     4201 acgatcgttc attgaaggaa gacgaaaaga aacaaatcat tcatactctt gttgcacaaa
     4261 ttcctattcc tgtgaggtag attgaggtag acaatggaaa aaatcaaaga ttatttcatt
     4321 aattttgtgt tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat
     4381 tctttaggct ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca
     4441 ttaagcagct ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc
     4501 aaaaaaagta tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacttctc
     4561 cctcgtttac aaatcggctt cccagcaatc tttgcagaaa aaaacaagag attttgattc
     4621 ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg
     4681 aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa
     4741 aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa
     4801 aattacagag agttctggaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgaaaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 agcttttttc acacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaagaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagtttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa tcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaat ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctggaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctttt ttcattatta tctgattttc aatgtcaaag catttagagc tacaactacc
     7681 gtacttaacg acatcaagac agcaccaact gctggactta atatgatacc aattggcgca
     7741 agaattccgg ctgctagcgg aattgcaaga atattgtaac ctgcccccca ccagaggttc
     7801 tggatcattt tctttcgcgt ttgcttggct agatcaagaa aatgcaaaat atcttttgga
     7861 tcgctgtttg tcaatacgac atccgcagaa tcgattgcga tatctgtccc tgcaccgatt
     7921 gccatcccga ttgaagcacg ggctaaactt ggcgcatcat tgatcccatc acccaccatg
     7981 attacttttt tcccttggtc aaggtatttc tggacaacag cttctttatc atctggtaat
     8041 aaaccgccat aatattcttc gatccctaaa taatctgcta ctgcttgggc agcgttttta
     8101 ttgtcaccag taagcatgac aggagtgatt tttctttctt tcaatgttcg gataaattct
     8161 tttgcttctg tttttactac gtcacctaaa gcaacgattg cagctagatg attttctaaa
     8221 atcaggaagc tgatggtatt tccttgttct tggtaaggtt tcaataatgc ttgttctacg
     8281 tttaatccga gacgctcaac ttctttttca ttaactattt tcacttgttg ttttttgact
     8341 gttgcctcta gcccgactcc tgaaagattt tttaggtttt cagcttgata aggcttgatt
     8401 tcatgttctt tcaaataatt cataatgcca acagccaatg gatgattggc attcgcttcc
     8461 aaggctccta gatattgaag gatttcattt ttattgaacg catcatcaag tacttcgaca
     8521 cccgttacag taaattttcc ttctgttagt gttcctgttt tgtccagcat gatcacatct
     8581 agatcattgg cttgctccag tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct
     8641 attgatgtgg aacgagcaac aactaatggg atagctaaac ctagagcatg aggacaggcg
     8701 atgataaaga ctgttaccat acgttctaat gcattaggaa gatctgtcaa gaacaaccag
     8761 gcgatgaatg ccaggatacc cacaattaat gcgacataaa acagccattt tgccactttg
     8821 tcagataatg actctagctt ggatttttct ccttgtgctt ttcgcaccat ttccataacc
     8881 ttcgctagat aaccattttc accagttcca gttacttcga tttcgatcgt tccatcccca
     8941 ttgatagaac cgccaatcac tttgtcattg gcttgttttt tgattcctct tgattcgcca
     9001 gtcacagctg attcgtcaac aattgtttca cctttcaaga ttacaccatc tgttggcatt
     9061 ttatcaccag accggacgat caaacgatca ccttcgttta cttctttcaa tgaaactgtc
     9121 tcttcttttc catctttcgt caagcgtttc actgattctg gcagtaattc tgctagtttt
     9181 tgtaaagcat tgctggcatt cgacacagca ttcatttcaa tccagtgacc tagtagcata
     9241 ataacgatca aagtagccaa ttcccagaag aaatccatga cgtgtgtatg tggattgagc
     9301 agatttgcga taaacgaata gacactataa atatatgcaa ctgtaatccc catcgcgatc
     9361 agtgtcatca tagcaggact tttttgtttc aattccatct tagctccact taaaaatggc
     9421 tgaccgccat agataaatag tatagttgct aagatcaata cgagccaatc agaaccagga
     9481 aacgttactt gaaatggaaa ttccattccc atcattggtg aaaaaagaat gattgggata
     9541 gctaggataa gcgatagcca aaatttttgt ttgaaattcc ccatgtgcat ggaatgatcc
     9601 atgccgccgt gttcgtgatc catgtgcatc gaatggtcca tttgtttgct gtttgctata
     9661 tctccatggt tatgtcccat gtgcatggaa tgatccatct gactatggtc atgtttcatc
     9721 tccatctgtt tttcatgaga ttctgtactt tctttgttta ctacttcttt tttatgctca
     9781 tgttttttct ccatatcgtt cccattcatt tttactccat cccttctctt actttttcat
     9841 gtgtcgattt aagctcaggg agttcaacaa tacactgacg gaactaaacg ccatcgctcc
     9901 tcctgcaatg attggattca aaaagcctag tgcggcaaaa ggaataccaa tcgtattata
     9961 gacaaaagcc cagaaaagat tttgtttgat ttttctcaat gtcaacctag acaactcgat
    10021 tgtttgtgca atggatgcca aactgctatt catcagcgtc acatcggctg tttccatcgc
    10081 aatgtccgtt ccacttccca ttgccatacc gacatcagct aatgctaagg ccggtgcatc
    10141 attgattcca tcgcctgcca taccgacttt tttgccatct ttctgaagtt tttctacgta
    10201 ggctgcttta tcctctggaa gtacttcagc aaagatatgc tttggatcaa taccaacttg
    10261 tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc agcataaaaa catctaatcc
    10321 tttattctgg agctgcttga ttgcttgttt gacttctagt ttgatttgat cagccacggc
    10381 aatcaacccg atcacttgct tttcatcagc taaaaacatg actgtttttc cctgctgttc
    10441 taattctaac gcgtgttctt ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt
    10501 acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg ctgattccag cacctgggtg
    10561 ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc agttttccgt attccacgat
    10621 tgctttacct agtggatgtt cagaagcttg ttctaaagta taaaaaatag ataggacttc
    10681 tttagaaccg actagatctg taactttggg ctttccttct gtaatcgttc cagttttatc
    10741 caaaacaatg ctgtctaagt gagcagctgc ttctaatgct tctccgcctt taattaaaat
    10801 tccatttcgt gcaccaacac ccgttcctac cataatagct gttggagtcg ctaaacctaa
    10861 tgcacatgga cacgcaatga ctaatacaga gacactatgg atcaatgcga gttgccaatc
    10921 accagtgatc aaacctgtag ctatgagcgt gataaacgcg atgactaata cgatcggaac
    10981 aaaaatccct gagatccgat ctgcgatttt ttggattggg gctttgctcc cttgagcatc
    11041 ttccaccatt tgaatgattt gcgctaatac tgtctgcttg ccgacttgtg taacttccat
    11101 gtgaagtaga ccattcgtat tgattgtccc gccaaagagc gtgtcatccg gattcttttc
    11161 tacaggaaga ttttcaccag taagcatact ttcatctatc gcactgcttc ctgaaataat
    11221 tcgaccatct gctggaatct gctcaccagg acggatgact agttgatcac caacgactac
    11281 ttcttcaatc gctaacgttt cttctttccc gtttcttatc acttgggcgg tttttgtttg
    11341 aagagacatc aattgtttga ttgcattacc tgttttcgtt tttgctgtat gttccaaata
    11401 tttgccgagc aaaatcagcg tgatgatcat actgctgctt tcaaaataaa gatcttgggg
    11461 gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag gcagccgatg ttccaattgc
    11521 taccaataca tccatatttg gtgctttcgt cttgattgca tggtacgcac ctttataaaa
    11581 acgagcaccg acataaaatt ggacaggagc agacagcact aactggacga ttggaaaatg
    11641 aaaaaagtga acaatcgccg catggcttcc taacatcata gcaatcattg acaacatcaa
    11701 cggcaaagtc aaaaccgtac tcaatataag atcgaatagc atttttttca aatacgcttg
    11761 cttttcttct gcaatttttt gtttgtgggc ttcatcgtac aaaattgcac catagccgat
    11821 gttttctacg ctttggatta atttttcagt ggtcgtccct tcatatttga cacttgcttt
    11881 ttcagtggcc aagttgactg tggcattgac cacaccaggt tgtccgttca gctctttttc
    11941 gatcctagca gaacaatttg cacaagtcat acctgtaatg acgaacgtat ccattttggt
    12001 ttctgctgtt gccatcaaat cacctctgct ttataaccca atccattgat tgcttgacaa
    12061 atcttttcgg attgaacttt tgtttcatca aacttgaccg taccgtttgc ttttttcaaa
    12121 tttactttca ctttttgcac gccgtcaagt gcgcttactg tttcttctac tcttgctgca
    12181 caatgattgc agctcatacc ttcgattgaa aattgttgtt tcattttttc ttcctcctat
    12241 tgatgttgtt tacattcaca ttgacctggg atacagttac attcgatcgt atctactggt
    12301 tgcttttgtt ccagttgttt caaaatctca ttgatatctt cttgagtcaa agtggcttct
    12361 tctactagat ccgcaatcgt ctctccaatc ctttttgcac agatatggga aaaaagattt
    12421 tccgtcgcac tgcgaactgt ttccatctcc gagacagctg gatgataaat gaattttttt
    12481 ccttcttgtt ctgtccagat cacttctttt tttaccaaac gacccagcaa agttttgatc
    12541 gttgctggtt tccaatccat cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc
    12601 tgtccaagtg tccaaataac acgcatgatc tcccactctg aatcactgat ctttactggc
    12661 gttttgactt tttccattta tttcacacct ttcgtttaca aatgtaatca ttatcttatg
    12721 aatacagatt acacttgtaa acaaaaactg tcaattattt ttgctctcta ttttcaattt
    12781 agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt
    12841 agctacttgg atcagctgct gtattttcgg ttcattgttt tcttcaaaat gccagataaa
    12901 agataaaaat tctaagtatt ccctgtttag tttctccgaa aatgattcag gcatatctgg
    12961 ccgagatttt ttgtttatct ttctttttag agaacgccaa actactcggt agatggcgag
    13021 ataacgcggc actttaagcc aaatgatctt atctgcttct ttaatccttt cctctaatgt
    13081 agaagtataa tttccttcaa cgatccatga cggattagat accataaaca attgctgttc
    13141 ttgcaaaaac cacttttttg cctgaatact gtagtctgtt gtatgccata aaaggtctag
    13201 cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt gcgagggtcg attttcccga
    13261 cccactttgt ccaatcacga tgatcttcat tcttctccct cacttctctt tggaatttgg
    13321 ttcgatcata ccacttataa cgccgcaaaa aaacaaggga ctgtgacaaa agtcgtgtca
    13381 aagtcccttg tttcgaataa atggtgttca atcagctgac cttcagcatt agctcaaaaa
    13441 gcggcaaaat atgaggagct atttttccgt ttgtttctct aatactcagg tcaaatcgct
    13501 gattttacaa cctcttcctt aaacggtgtt ctcctgccaa cacctcggtc acaagtcaca
    13561 atagtcaaca aacatgagaa gttgtttttt gaaattgctc cttgtgactc ggagttaaac
    13621 ggcaggctca ctacctcttt cttaaacggt gttccaaagc ttgcatctgc ggtaataagc
    13681 ccagccaaac gaaaaatatg aggggctatt tttgtttggc tgttcttatt acttgatgca
    13741 ggacagcttt ttcacaacct ctgattcacg gtgttccaga agtagcttct tcgaaaaaca
    13801 agccgaaact tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    13861 gctgaccact tctgtcacaa cctcttgtaa caagtatttc tattttaagg ctgaaccact
    13921 gtttctggtt cagtgatttc tttgtttttg tctcgacgag caattgctgg caagatcata
    13981 cctaataaga tcaagacgat tggtgtgatg atattcgtaa ataattggaa ccaccatgct
    14041 ttaggatcag cagtataatc tacttttggc accattccta ggatacatgc aaacgcagtg
    14101 aaggcaaagc accaagcacc aaagataaaa ccgatttttg gatttttaac aaatttatat
    14161 tcagaattga atttcttata agctttattc aacatcatat atgccaagaa cacccatagg
    14221 taacgcattg gcatgacaac tgagttcaag ttcgtcaacc attttactaa ttcattcata
    14281 tctttgatac caaaaatcgg caacaaaata atgattccta ctagtatacc tgtcagaaga
    14341 taaccattca ccaatgtacc tttacgatta cgtttacgta gccagtttgg tacaaattct
    14401 ggatccgcat ctgctaacaa gatttttagt ggtgcatcaa ttgaaaaggc taatgcggag
    14461 atttgtccaa tcatgtttgt caatgcgtaa atgatcatca atgagttacc gataccatag
    14521 taattaccta aaatttgaaa agcactgtat gccccatttg ccatcaagtc tttaggaata
    14581 ttatcactag caaacagcat acccattgcg actgagccta atacagcaca aatccctacc
    14641 attgctgcta agaagatcat ccctttagga aattcctttg caggatttct tgtagagttg
    14701 acataagggg atattttttc cgcccctcca actgcaaata ctagcatcga aacggttgtg
    14761 aaataactga aatcaaatgt tggaatatat gttttcactt gattcatatg tggtgtggca
    14821 aattccatcg ttggattgat gaacggtgcc gttacagcta aaagaataaa taagatcgac
    14881 atgacgaaca tcgctgttcc ggccagtccg ccaatcactt tcaaagtaga aagtcctttt
    14941 gttgataaat ataaaaaggc aagaaatata ataagtgaaa taactgctac catttgtact
    15001 gacatcgtat ttacgatatt tccatttcct tggactgccc aaccaccagc aatcagaatt
    15061 gcttgaggct tttgtgccaa ataaggaata tggacgaccc agtaagtcca agcggcatag
    15121 tatgccagtc gtttattaga tgtcgtattt tcgacccatg aactgacgcc tcctttacta
    15181 tccttgaagg ctgatcctaa ttgtccaacg atcaatgcgt atggaataaa ataaatggcc
    15241 aaaatcaata gccaagaggt gacaactgat actccttgct gagcatagtt attaactaca
    15301 ttccccatgc cccaaaccat gttaaatgca attaacgcaa cagtaaacca gcgtaattgt
    15361 ttgttgttca ttcgttctct cctttatgat aaaaatttgt tatccagatg taatcatatt
    15421 caatttttaa caaacttaca agacagtgtt attttatgtg tgtattcttt tatttaatac
    15481 acatcgtttt ttcatatttt ttccatcaaa aaaataagtt attttctttt atttcattga
    15541 ccttattgaa tttcaagcta ttttgaattt tctgcaaaat taaaaacatt agttatttta
    15601 taatcctatc tattttcttt acatttttca ttaagaaaag gatataaaaa aaagaggacg
    15661 ttcattcgtc ctcttcattg atcaacgttt attttttaca tgcaagcttc gcatggcatt
    15721 caaaacagct aatactgtca ccccaacatc tgcaaatact gcatctccca ttgatgcaaa
    15781 accaaaagca cctaaggcaa gtaccaaaat ttttatccca atagcaaaga caatgttttg
    15841 tttgacgatt ttcaatgttt tctttgctaa atggattgct tctgcaatct tcgatggttg
    15901 atcattcatg atgactacat ctgctgcttc aattgcagca tcgcttccca atccacccat
    15961 tgcgattcct acatctgctc ttgctagcac cggtgcatcg ttgattccat ctccgacaaa
    16021 tgcggtcttt ttattgtttc tttgtaagat ttcctctaac cgctgcactt tatcttcagg
    16081 aagcaattcg ctatatatct tgtccacgcc tacttgcttc ccgatatgat cagcaatttt
    16141 tttcgagtcg cccgtcaaca tgactgtgtt cttcacacca gcctgtttta attgaaccaa
    16201 tgcgtctacg gcatcttctt tcaaggtatc tgcaataaca agataaccac tgtaactttg
    16261 atcgatcgct agataaagca atgtaccgat ttcttgactt aaaggaaaag agatttcaaa
    16321 tctttccatc aatttgtgat tacctgctaa tacttgtttg ccatcaactt cagctaagat
    16381 cccttcacct gcaaattcct ggatattagt tgttggtaaa agcggacctg cgtagccttc
    16441 taaaacagat tgagcaatcg gatgtgtaga aaactgttct gcactcgcaa tatattgcat
    16501 gaatttcttt ggatcgattt ttgtatcgat cgtctggatc gaaaagtcac ctttcgttaa
    16561 tgtgccggtt ttatcgaata ccactgtctc ggtttgcgca agcaactcca agtaatttga
    16621 accttttact aatacaccga ttttacttgc gcctccgata ccgccaaaga aactaagtgg
    16681 cacggaaatc actaatgcac atggacatga gatcactaaa aatgtcaatg ctcgatagat
    16741 ccattcagaa aacgccgctc cagaaacaac aagtggaggt aaaacagcta ataaaacggc
    16801 caatcctaca acgactggtg tgtaatagcg cgcgaattta gtaatgaaat tttcagctgg
    16861 tgctttttta ctgctggcat tttctactaa ttctaataat ttgctgatag tagaattttc
    16921 ataagatttt tctacttgaa ctaaaaatgg tttattttta ttgataaatc cgcttaatac
    16981 tgtttcacca acatatacag atttagggac tgattctcct gtaagtgctg acgtatcgac
    17041 taatgattct ccttcgatga tcgttccatc taacggtaca cgttcaccag gcttcacaag
    17101 tacaaactga ccgatctgca cgtcttcagg gttcacggtc atcaatcctt ccgtcgtctg
    17161 cacgtgagct gtttctggac ggatcgccat caattcgcga atcgattttc ttgaatgact
    17221 gactgccaat ccttggaaat actcgccgat ttgataaaat agcatcactg ctacggcttc
    17281 tggatattca ccgatcaaca tagcgccaat cgtagcaaca gacataagaa aattctcatc
    17341 aaagattttt cctttgacga tattcatcac tgccttcttc actacatcat atcctatcag
    17401 cagataaatc aataaatacc ctatcaagcg aattggttca ttaaaatcaa ataatagcaa
    17461 taaagcgata cctgcaaggg aaaataaaat ccggaacagc gtatttctct ctttttcatt
    17521 tccatgttca gatacttttt ccttatctat ttctgttact ttgacatctg gttctaattc
    17581 cttcactact ttttttgttt cttgttcaat cgtagccaaa tgatcttcct ctgcgtcaat
    17641 cgttagtttt ccagaagtga agtttaccgt tgcttccttg actccattga tcttctgaac
    17701 tcctttttcg attttcattg cacagttcgc acaatctaaa ccttctaatc tgtaactttt
    17761 cattttaatg acctcctcgc ctcaacatat gaatatctct tcataactaa ttataaatga
    17821 atggttattc atatgtcaag gtatccgaaa gaaaaaatga aaaacatctg catcggaaaa
    17881 tttcttccat acagatgttt tggcttattc tttctttttc tcttcgatca ttgcccctgc
    17941 tcttatccaa cccgcacttg ccttcttgat cttgacagga tagcaactaa gtttgaagcc
    18001 tgttgctggc aatttgtcta aattcattaa tttttcgata ggacaatatc ttttttcaat
    18061 ccctgcaaaa tgtccttccc agatattcga agctccacta cgctttcaaa aatatagaaa
    18121 gagttcttta atgaaaatta tttcaacttt ccataaacag taatcccata taaataaaag
    18181 acgtaaaagt cacatgggag aacttataca ggaaaacaca tagagtcagg tgaaatacat
    18241 gtatcccacc tgactctatg cgttttctaa cctttattat tctcttttta tacaatttct
    18301 gcaccgaatg gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc
    18361 cctatgatcg tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg
    18421 cctgaaacga tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt
    18481 acttctttgc caaaaggcgc aaaagaaagg gaggcgatct taaaacattg cataccgatt
    18541 gggataccta tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg
    18601 attagtccgc cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt
    18661 ctattactgt ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt
    18721 ccagcatcaa gtattctgca aatttttttt ggatttcttc cgtattttct tttgttacta
    18781 atgtgtgttg gattagtttt cctgatggtc gctgatccac ccgttcttct gactgttgca
    18841 cgtccgcttg cgcaaatccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt
    18901 tattcttatc atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag
    18961 taatcagctg attgatttga ggtaaatctt cttttactgc ccaccgtatt tccatcattt
    19021 ctcacttttc tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc
    19081 tatccagctt tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct
    19141 tgtatatttc gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac
    19201 cagcaatgcg ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc
    19261 tttacgccag ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat
    19321 gttgcagatt tccgctgttt gaagttcatt tagtgatttt cgataaacgg attcaaattg
    19381 ctgcgcagat ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt
    19441 gattccgatc aaaatcgata catagtcagg ttgtaaagct aaacaatctt cttgccagcg
    19501 tacttgcaga tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat
    19561 cgttggatat tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc
    19621 agttgcttct gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg
    19681 ttttttcatt tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat
    19741 cccataagcc ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag
    19801 tcccttcaat caattgaatg aacgctcatc aagtgtctag ttttttatac tttttttctt
    19861 aacgtgccgg attccccttg attccttcgt tgtttggtcc agagaaatcg atcacaccta
    19921 agcttccgtc aatcagacca ctgatgacgc catcagaaca tatatttata ggctcatcta
    19981 agacttccag taaatgttct tttactaata tctcttctag attgcttggg atagattggt
    20041 cataacttta tctggtccga tgatttcaag gatgaattaa acttacatat ggatcattgc
    20101 atgctgaaaa tacggaccta ctccatcttc gaatgttact attagtttgt tttcaggtac
    20161 tagatgctgc tttatttcaa cagcagaagc atcatcaaat cagatttcca tttgaataac
    20221 cttctttgta tatgtttgat tgattgcgat ttcgttatag aaccttgtga atcaaatagc
    20281 aatttctttt gctttcttct cttgtctgct ggaaacttcc tttatcccgt attttaaaaa
    20341 aagaactgcg agagttcgca gttctttttt ttaaataaac gttgctctcc ggccaactcc
    20401 tcggtcacaa gtcacaatag tcatcaaaca tgaaaaactg ttttttgaaa ttactccttg
    20461 tgactcggag ctgaacggca ggcgcacaac ctcttgatat aaacggtgtt caatcagctg
    20521 accctcgata ttagctaaaa aaacggtaaa atatgagaag ctatttttcc gttttgtttc
    20581 tctaatactc aggtcaaggc actgatttca caacctcttc cttaaacggt gttcaaaagc
    20641 cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt ccacaaaatt
    20701 tttcttaata ctcgaaacta aacagctttt tcatcacctc ttatggataa attcggttca
    20761 acaaacgtgg gaatggaata gcttcacgta catggtcgat accagcaatc caagtgactg
    20821 ttcgttcaag gcctagacca aaaccagagt gcggaacaga accgtattta cgtagatcaa
    20881 gataccatga atattctttt tcatccaaac cgtgattacg gatttgttct agcaagaaat
    20941 catagtctgt tgcccgttcg cttccgccaa taatttcacc gtaaccttct ggtgcgatca
    21001 tatctgcaca aattaccacg tcttctcttg ttggatgtgg tttcatgtag aatggcttga
    21061 tggcttttgg atagtttagg ataaagacag gctgttcgaa tgaattcgca atgaacgttt
    21121 catgaggtga accaaagtct tcgccccatt cgatgtcatc aaatccattt ttcttcagta
    21181 attcgattgc atcatcgtaa gtaatacgag ggaatggcaa ttttgtataa gtttttaaga
    21241 cttctttgtc tcgtcctagt acatctaatg catattcgca atgatctaaa acattttgta
    21301 ctaagaatgc tacatattgt tcctgtactt ctaaactttc ttcttgatga gtgaaagcca
    21361 tttctggttc gatcatccag aattcgatca aatgacgtcg tgtttttgat ttttcagcac
    21421 ggaaagtcgg accgaatgaa aagacttttc cgaaagccat agcagcagct tccatgtata
    21481 attggccact ttgagataaa taagcttttt gatcaaagta atttgtttcg aatagatccg
    21541 tagtgccttc tggtgcagaa ccagtcaaga tcggcggatc gattttgaca aaatgatgtt
    21601 gattaaagaa ttcatacgtt gcacgaatga tttcatttct gatctgcata atcgcatgtt
    21661 gtcttgaaga acgcagccat aaatgacgat gatccatcaa gaaatctgtt ccgtgttctt
    21721 ttggtgtgat cggataatcg tggctctcgc ttaccacttc cacatttgta attccgattt
    21781 cataaccaaa ttttgaacga ctgtcttcac ggatttctcc agttactaac acggatgttt
    21841 cttggtgtag atttttagct gtttggaaaa cttcttccga cacttcgctt tttacaacta
    21901 caccttggaa aaaggctgtt ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc
    21961 gcttatttgc gacccaagcg ccgattgtta ctgtttctcc cacatggttt ttcgaatcga
    22021 tgatttgaat ttgttccacg tatgactctc cttttatatt ccttgaattt cttcaggatt
    22081 aattttgact tttactctca ataaattgct tgattctgcg taccgcttct tctaacgtag
    22141 cgcgatcagt ggcataactc atgcggacat tttctggtgc cccaaagcct tcgccagtca
    22201 caagcgctac ccctgtttct tctaaaagat cttctaccca ttgtgtgaca ttttcatact
    22261 tacacatgcg tagcgtttct tttacattag gaaaaagata gaaagctcct tgcggctttt
    22321 caagagaaac acccggcagt tctgccacaa gcggatataa cgtattcagg cgttcttcaa
    22381 aggcgttccg catttcttct actgtatctt gctcaccttt caatgcttca acagctgcat
    22441 attgactgac tgctgtcgga ttgctagttg attgggaagc aacagcgatc attccattga
    22501 tgatcacagg atttcccacc gcataaccaa tgcgccagcc tgtcatcgca tatgttttag
    22561 acacgccgtt gatgatgatg gtctgatttt tgattgcttc tgaaatcgtt gcaattggag
    22621 taaactcatt gccattataa actaaacggc cataaatatc atcagcaact atcaaaatat
    22681 cattttttac tgcccattca ccgattgcct gcagttcatt cttgctataa atcattccag
    22741 tcggattcga tggagaattg atgattacgg cttttgtttt ttctgaacgt gcttgttcta
    22801 gctgttcaac agtaactttc cagctattgg attcttcccc tttcacaaag acaggaagtc
    22861 cttctgccag ttttacttgc tcaccgtaac tgacccagta aggcacagga atgatgactt
    22921 catcttgcgg gtcaaggatc gtttggaaaa gggtatacaa agcaaatttc gctccgtctg
    22981 ttacgactgt ttgtgaaggt tcatagacaa gtccatagtt tttcttcaga taatcaacga
    23041 ttgcctgtcg aagctctggg attcctgctg ttggtgtata atagctagct ttcccattgc
    23101 ggattgcttc gattgctgct tcttggatgt tttctggtgt cgcaaaatcc ggttctccca
    23161 cagtcaaact cagcacatct ctgccttttg cttttaatgc cttggccttt gccgcagctg
    23221 ccaaagtgac agacggttct aatcgttttg ttctttttga taactccatc ccattcattc
    23281 ctttaccttt taaattccct tgattgtctt gacttcatct ccagattcat agccaatcag
    23341 gtaataattc agtttcccat ttgtttcttt tcctactact tcccacacag gctgatcatc
    23401 atacatccct aaagcagctt tttcgatttg gatttcagga tgggccgcag caattgtctg
    23461 ttttgcttca ttttcagtca gtccctttgt ttgatccaat acttttactt tttcaccaga
    23521 tttaggaatg ataacggcga tttcttttcc tttatcattt gtacctgtca gtgaaaagta
    23581 tgtcttcttt cgagtaaacc aataaaaatg atcggcttcc tcaagatttg catatttctt
    23641 tgcgatttct actgcttctt tcttcgcctg caccgccgga cgactggaac gcaagtaaaa
    23701 aatcgagctg atcacgatga ccgctaataa aaatacgatc aagccaagta acacgcgatt
    23761 aatcgttttt tcctttgtct gttttttctt caagggtcgt cgctccttta tcttcattcc
    23821 ttttcattat agcagatttt aattatgaaa aaagacagct cctctaactt ccttcttcat
    23881 ttttctttaa gaattcatgg atatcctgat agatttcctc catagctagc tctttgatcg
    23941 gtactttttt cggtaatgca tggatgatcc gtgcaccata ttttgcagta actagtcttc
    24001 gatccaaaat aagcaatacg ccacgatctg tctccgtcct tatcaagcgt cctaatgctt
    24061 ggcgtaactt caaagttgct ttaggtacgg attcctggta aaaaggattg atcccttctg
    24121 attctaaata agcgttccta gctttgacca tcgggcgaag cggattatcg aagggtaaac
    24181 gtgtgactac aacgatctgc aaagcatctc caggcaaatc tacaccttcc caaaaactgt
    24241 cagcgccaaa caatacgctt ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc
    24301 ttccgccaaa cccttgagct agtatttctc gtcctttttc tagcatagac agatgcattt
    24361 tctgatagac cttctgcaaa acatcatgag aagtgaataa cacaagtatt ggccgatctt
    24421 cttgttcgat cagacctgta agtgctgtct ctaaataatc gatatatgct tgattgctga
    24481 cttgatggac ggcaggtgcc tcttttggca catagatcct tgcctgtttc tcataatcat
    24541 aaggagaggc aatcacttta agaggtgctg cttcaatgcc aagcctctta gagagatagt
    24601 gtcgattagg gccgatcttc agcgttcctc caatataaag aatacgatca tatcgaggat
    24661 accaaacagt cgacggcaac atagccgctt gaaaatcatg gagatgaaaa atcatctgct
    24721 gcgctgtgtt cccaggaacg aaccaatgga tcaaatgatc tcgccagtct tccagccatt
    24781 gatccatcag caagtactgc ttgtctaatt gatcaaatag gctatataaa cttgctaaat
    24841 cgatccgcat ctggtttgtc cagctttctt ggataatctc aaaagtcaga cgcatttgtg
    24901 actgtagctg ctggatctcc tgataaaaaa gctgcaggtg atcaatggct gtcccagatt
    24961 ccagtggcag ctgatccaac atctcttttg tcatcatgac ttcttctgga tactgtccgt
    25021 tgggttgctt gccgctcact aaactcatta atccttctgc catacaaatc tgtgcttcgc
    25081 atagcgcaga aagttcttct cgataaatcg ataaccagcg taaggcttca tgttctttcg
    25141 gtaaatagct ttgtattcgg tcaaacagct gatttggttc ttctaattgc tggatttgtt
    25201 tatggaaata gttaatactt aatcgtttgt catttacttt ttcagcgatt tccggcaaat
    25261 gatgtacttc atcgatcaac aaaaatggac tagctggaag caatggttct tttcgctgtg
    25321 tttcttgtac taaaaacgca tgattgacaa tcaaaaaatt actttgcctg attttctttt
    25381 gcaagaaacg aacaaagtct tgttcgtaaa atgactgatt tcttgctaaa aatccaatac
    25441 ctctgtgcgc gatatctgca aagaatgaat ggttcaaatt tgttaaatgt aattcatcca
    25501 aatcaccggt cttcgtttgc gtcagccaaa ctaagaccgc catttgatac aaagcatatt
    25561 gtttttgttc tggcggttcc acaagtgttc ctttaaatcg ttgcaggtcg atatagtggc
    25621 gatagctttt gataattaca ggttgtatgg gctgatctag caattgattc agcaatggaa
    25681 tgtctttttc tatgatctgc tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt
    25741 tttcaggtgt agccaaataa ctcaatggta gcagataacc gattgtttta cccattcctg
    25801 tagctgcttc gatcatcaaa tcttttgatt catcttttgt gaaatggtcg tagaccgcat
    25861 tcatcagtct gttctgttct tttcgataca tcagtgtttt accaaacatt ttttcttttg
    25921 cttgtttttt tcttggatat gtccgctcgc cataatggac ggatgtgaat agttccactt
    25981 cttttttctg taaagctagc ccatcgacga tttctaacga gggatcaagt ggctctggtt
    26041 tttctttcat ttcttcaatc acatgataaa tgaaacgact cgtatccata cctgtgtgtc
    26101 tacttaatcg cgcaatctgc tctaacgtga tgattggcaa ttttctcatc cgttcttcga
    26161 tcagcaaaag aagttgacct gtcacttcgg catcactatc tgcctgatga ggattctcat
    26221 gaataaatcc aaggctttct gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga
    26281 aaatttgtgc aagttctact gtatcgatgc ctggtatctt caaactagga agtccgcatc
    26341 gcatgagttc atggttcaaa aaattataat caaaataaat attatgcgcg acaaatatgg
    26401 tgtctgccaa aagattgtaa atcgtttggg cgacatcttc gaaataaggc gctttttgga
    26461 cgcgttgatt cgtgatccct gttaaatgct ggatttgttt tgaaatcttc cgccctggat
    26521 gaatatcgat cgaaaaacgt gaagtgatcc gacctgattc aatcatgacg cagccaaatt
    26581 ggatgatccg atcttctttt ggattcgtcc cagtcgtttc gatatcaacg actgcataag
    26641 ttcttggttt caaaatatct tcctcttctc ctgcctctaa tgttcttttg aaattatact
    26701 acaaagtgca gaaaaatact ctcatcgctt tttaaagaaa atagacaaaa acgcccagac
    26761 acgcaaatgt ctgggcgttt attttttagt ataggttaat taagctttag taacgtttgt
    26821 tgcttgaggt ccgcgttggc cttcttcaac atcgaaagtt actgcttggc cttcttctaa
    26881 agttttgaat ccgtcgcctt ggatagctga gaaatgaacg aatacatcgt ttccgttttc
    26941 tgcagtgatg aatccaaatc ctttttctga gttaaaccat tttactgtac ctgtttccat
    27001 aataaaaatc ctccttgtgt atcaacacat attttgcaat tacttgaatg gtgtatatct
    27061 ggaagatgtt tatttgaaac aagctaccac agattaccga acaaaaacta ctcctctagt
    27121 atatcatacc gtttacaaaa aagctagagt ttttcgactt tttttattaa aaaaagtttt
    27181 ctcagcgatt acataaagcg aaaaaccgaa ctttttttaa aataaacgac atattgttca
    27241 gttggtgttt ttttgctctg atgttctttt ttaaactaaa aaaattattc tttctgacta
    27301 cttatcggtt ctgtttcaac ggtcgaggct ggggaagatt ctttagtagg tctgatgttc
    27361 tcatggtatt tctgatagcc tatgacagtt accagaagaa caaggcaaac caatacgatc
    27421 aacgagatta gtttttcttt tttgctcata tctatacctc ataaataaca ggatgatgtc
    27481 catcatattt ctgagtatga gtaaaagcaa aagctgcagc tgtagaatgt aagatatgcc
    27541 agcaactttc ataatcacag gtcggacagt accaaaagta accaaatctt ggctgcaaag
    27601 accgttcttc tgtttcagga atcgcagaat caagatctgt tgtctgtaca taaacatttt
    27661 tgtacagacg tgtgcctgta ataggagacc gctgtgtttc ttcagcaaaa actggctgac
    27721 ttgttaaact tagggataga aacagcaaac accataacag tctttttttc ataaagacct
    27781 ccttatcgat aagtttttcc caattaaaat catatctcaa gaacgcccgt tattcacatt
    27841 ttgttcataa atgatgtttt ttgtatcata atcgtcaaaa agaggtcgtg accacgtcac
    27901 aacctctttt ttaaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttcac
    27961 aaaaatttga gaaataattt ttgtcaatct cttcttattt cttgaagctg accacttctg
    28021 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaccaa
    28081 acaaaaaata tgaagagcta tttttgcttg ctgttcttat tacttgatgc agaacagctt
    28141 tttcacaacc tctcatacac ggtgtttaat cagctgacct tcggtattag ctcaaaaaac
    28201 ggaaaaatat gtgaaactat ttttccgttt gtttctctaa tactcaggtc aaaacactga
    28261 tttcacaacc tcttttaata acgaagaatc gctgtcagac tcttttcatc tggcgcatct
    28321 ttttgatcca aaataaacac tttcccgcca ttaatgatca catcatctgc taatgcattc
    28381 aacacttgac gccgatcata ttctgtttca ggatcttcac cgaaaccatc agctaagttt
    28441 gacgtagcga taaataaatg tgacactttt ccttcttttg cagctggaac gatatcaact
    28501 aactgatcga tgaatttctt atctaagagt ttattgtatt cttttgattc ttgagctgcg
    28561 agttcacttg acagcttctt cgccccttct tcgatatctc taaaggatag ctgcgcagga
    28621 gaagcagaaa tcgcaacgtc tgcacaatag taaggatttt tggcgatttt tttgaacatc
    28681 gtctgatttt ctggtagagc aaataaataa atcggccatt gttctggatt ttcgaaagaa
    28741 tccttcaagt acgtatcaac tgcttggtaa tagttgaccc aatcgatctc tacttcttca
    28801 tcttttggat ttacgccatg gaaagttact ccatctcggg ctccgcctcc tccattagaa
    28861 gagaagttca aaccgccacc tgtcagttca gatcccaatg cagtaacgac atcaaccggt
    28921 gccccttctg gtagagatac ttcttttact tgtttgttgc tgacttcata caatttcatg
    28981 gagtcgcggt tcaatcccaa taaataataa gaatagttga actgcccatt tttgataata
    29041 gccagtaaat aaggaagatc acttacatag tattgatcgt ctaccgggat actcaaacga
    29101 tggatatagg tttggtcagc agttataatg accgctacac tcgtagttgc actacgccaa
    29161 aacgaaggat ctcctaacaa attttcaatt ttttcttgga acggggacca gtcatgttcg
    29221 gaatattttt tctcaaaacg tttctttgct tctttcgaaa aatttttgaa tttgatctga
    29281 tctttttcca catcttgatg ggcgacatgc gtattcagca tgatcgtgac gaatggtcct
    29341 ttagcgcctt ctgaaatcaa ttgtgacaat aattctttat ccttttttgc cataaacgta
    29401 cccctcctaa aaaaatcatt cctttgtttc tatctaaagt ctaccacaac actatttttc
    29461 catacaataa aacgtatcta aaaaaataaa acggtttcta cggttttaaa tttagctatt
    29521 tttttaagtt atcttcataa tttcctcaat tatttagccc cagtcttcaa ctattcttca
    29581 tgaaatgctg ataaactaag aaaaagaata aaagaggtgt atcttatgca tttgattatg
    29641 attttcagtt tattaagtat tatcagttta tttgctttca gcgcttcccg cttattagag
    29701 gcggaaacac agcctgttcc tataaaagta cgaaaaaatg accgctaacc ttttcataac
    29761 gcgcgttctt atcctaaagt ttttttcaga aacattgaaa ctctcttcct tctctgataa
    29821 aattttaatt tatagcgaaa caaattaaat acaaggagat cggatatgtc cataaaaaaa
    29881 acacgtctag tcctattatt ggctagtctc attctttgcc ttgcttcttt tctaccagct
    29941 gttgctgtgc aagctgaaga tacgttcaaa gtaaatgcaa aagcagcttt tgctgtggat
    30001 gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa aaaccatggg gatcgcttca
    30061 atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag taaaagaagg aaaactatca
    30121 tgggatgata aagtttcgat ttcagattat gcagaaaatt tgagtatcac cccagattta
    30181 tcgaatgttc ctttgcataa agaaaacacc tacactgtca aagaattatt cgactctgca
    30241 atcatccagt cagccaatgc ttcaatggtg gccttggcag aaaaagtctc tggcagcgaa
    30301 gctaaattca ctgaacgaat gaaagaacaa ttgaaagatt ggggaatcaa agatgcaacg
    30361 atcgttaatg catcaggatt gaacaactct tatcttggcg aaaaccgacc agaaggcact
    30421 ggagaaaacg acgaaaatca aatgtctgct caagatgtgg ccatcgttgc ccgccatttg
    30481 atcttggatt tcccagaaat actggatgta tcaagtacta cgacccaaat gtttggcgaa
    30541 aatacgcaat caccagtgga aatggtcaac tggaactgga tgctcccagg attcgttaat
    30601 tacaaggaag gcgtggacgg attaaaaact ggtacgactg agcttgcagg tgcttgtttc
    30661 gttggtacga tcactaaaaa cgatcaacgg atcatcactg tcgtattgaa tgcggacggc
    30721 catgcggaaa acccgagtgc ccgcttcaat gaaacagacc gtttgatgga ctactgctat
    30781 gacaattggt cagaaaaaga gctcggaaaa gctaacgcaa gtattcctag cctaaaaacc
    30841 attgatgtaa aagacggaaa agaatcaaca gttcctgttg tgcttaaaag ccctgtaaaa
    30901 gtatgggtac gaaacgatat ggatactggc aaattaacga tcacgccaac acttgacaag
    30961 aagcaagtca ctgacaacga gatccaagca cctatggata aaggtacaaa aatcggtacc
    31021 gctacgatca ccttggcaga cgataaatta ggttatttag aagataacaa tgcgccatcg
    31081 acagagatca ttacggataa aacagtggaa aaagcgaata tgttcgtgat tggctggcga
    31141 cgcgtcgcag aatttttcgg caacttgttt taattgaaca ataagaaaag catctttcca
    31201 tcggaaagat gtttttttgc tttttctagt ttaataacag cttttttcat ctattttcgt
    31261 tatactgtat aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta
    31321 gttctctatc ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct
    31381 tccttgttct tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg
    31441 ttggaaattt tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt
    31501 gctttttctt atttcacaga aaaacgaagg ctgctgaatg gtcttttatt caatgttttc
    31561 ctcatcagtt tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tattttcctt
    31621 ggcggactga tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat
    31681 ggattgattg tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca
    31741 ttagctaatc ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac
    31801 tttttcttgt taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg
    31861 atcttgatct atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag
    31921 ttcaaccggc ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg gttgatcaat
    31981 ggagaaacag tttctcctct tctagccaag cgaatcaata aggcgattgc tttttatcgg
    32041 gctcaaagcc gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca
    32101 gacgaaaaag taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct
    32161 gaaagagaca ttcttgtcga aaccaattcg acaaccactt tggagaacat gctctattca
    32221 aaggaaatca tggatcagca aatgaaaggg ccataccgcg tgatctttag ttccaacaat
    32281 taccacattt ttagagccgg tctttatgcg tgtcaagcca aattgaaagc aaatggtatt
    32341 ggtgcaaaaa cagctttcta ctacttgcct aatgcgtttt taagagaata catcgccatc
    32401 ttgatgatga acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca
    32461 ttcttatcgc tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac
    32521 accttgtttt tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc
    32581 gctacttgct gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag
    32641 atcataaaga agtatactgc agcttgtgaa ccgaacaatt cgatccccat cacaaagcag
    32701 gcaatcggtg tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa
    32761 ggaacagaaa catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac
    32821 aatggtgtca cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac
    32881 ttcccaataa aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga
    32941 ctcaatccga gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca
    33001 cctatcgtat ttcgccaaac aggatgagga atccattgtg catatcgagc ttttaaaaag
    33061 acgatcgaac ggctgaatac ccagccaatc aaaccaaaag caatcccagc aacaaaaact
    33121 ttgaagaaca gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa
    33181 acaccatagc tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc
    33241 gcttctgttc ggacttttcc gattgccaac acttctaatc caaataatgt tccagctaaa
    33301 ggtgtgccaa atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt
    33361 tctgatttat ccaatcggaa caaacgtgct gtattatctg ctaacgctcc ccccatttgg
    33421 actgctgttc cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac
    33481 agtgtaagtg gaataagtcg caacgggatt ttttcttctc caccatttcc ttgatcgatc
    33541 actaagttgt ttccacgact agagagaccg ccgtgataca catagagata agcaaaaagc
    33601 gcaccactaa ctggtagtag ataaaggagc caaggatgtg atagtcgggt atctgtcaca
    33661 actgtcaagc tatttaagaa aaaagcagat aagctcccca tgaatatacc gagaatcgct
    33721 gtgatcacca gccacttcag catatacact aaaatgcgca ttggactcgg taattgtttt
    33781 tccataaaaa attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga
    33841 ctttagtagg cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat
    33901 tcacttccat taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc
    33961 tttacacgtt cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg
    34021 tcactatcat gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc
    34081 gttgtagaaa gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta
    34141 tttatcgttg gtgataaagt accactaatc ccttttaatt tttcatcttc gtaatagacc
    34201 atgaattttt tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg
    34261 atttcattcc aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt
    34321 ggtttgctag gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct
    34381 gcttgcgctt ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gatttctagc
    34441 agttcgtgat ctaatatgtc tcgaatcttt tcagctgcta gtgcctgaac tgtttgtacc
    34501 gtttttggat cattgacaga tgagtacgca tttaataact gttctacttc tctttttata
    34561 aaaaaatatt ggtatggttt tattgtttgt tccatgattt taaagtcctt tcttctaacc
    34621 aaagtgcata ctttgtagaa aaaagtgatc ttctgcaaag tatgtgttac ctgaagatcc
    34681 cggactcttt taaataagat acgttttgca tcttatcatc acccccttta atgcttattc
    34741 ttctatgtac atgattcatt cgataaataa aagtaaaccg tatcttgaac agatagtcaa
    34801 ctgcaaacag aaaaaaggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaatgaa
    34861 tggtgttcaa aagcttgcat ctgcggtaat aagccgaaac ttcacaaaaa tttgagaagt
    34921 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcaatat
    34981 ggtctattat tggttttacc tgttaaatcg aggattcttt cccaaagtaa cgttgccatc
    35041 tcttacgcat acttgcagac atgggctgag ccgcttcttt gactgcgcag tatttgtcca
    35101 cgaatgtcac gatatatcct tctttgtact taggcggtgc gatcgttgca ccccacatgt
    35161 gcttcaaaat gatatctcgc tcaagatccg acaaatcggt gattttttct gcattcttta
    35221 cagcaattct tggatggata tacgcatgtg ttccctcgtc aaatttcgtc gtacgccaat
    35281 catagtagaa cagatcatgc aaaagtccag cacgcgctgt tgctttagca ttgccacccc
    35341 atttttttgc gagcagataa ctatagtaag aaacactgat ggaatgctcc aaacgagtgg
    35401 aatgaacatg ctgtgtgtaa ttcgctaatt ttttcactgc ttctgtttct aaaagatctt
    35461 gtacataaga catatattct tcgtcttcac gccatttttc tgtcataatt atcgatctgc
    35521 cacctttttg aatttcattt tcttctgaaa ttgatctaag tataccacca gtgttcggat
    35581 tattctagtc gctctagata aaatagctaa agcttaatag ttccttaatc ataaagaaaa
    35641 atgataaaga acgatccctg ctgcaactcc gacattcaat gattccgcat ttcctttcat
    35701 tgggatatat acgttctgat cagtcaaaga caagatttcc tgacttactc cctgaccttc
    35761 gtttcccatg attatagcga aatcctctgt tttttctact ttgtctaatg catgtgcttc
    35821 ttcatttaat tctgtaccgt acaccgtaaa ccctttgcct ttcattacag ggattacatc
    35881 tgccaaatct ttttgtaaca caggcaaatg ataattgctt ccctgcatgc ttcgcagcac
    35941 ctttgtacta taaatatctg ccgttccttt acctaataaa acaccagcca taccaaacgc
    36001 atctgccgta cggatcatcg ttcccacatt tccaggatcc tgtacattat ctagtaaaag
    36061 ccagcctcct gaaaaatctg gcgtatcttc agatgttaac gtcatcactg ctaagattcc
    36121 ttgcggtgta ggtagatctg acatagcggt catgacctca tctgaaacta gaaaagtaga
    36181 aatctctttg tttacagcca gccagtctcc ccattctttc atacctctct gtgtcacaaa
    36241 aagttctttt atttctgcat cagacgacgc agcttcttcg atcagatgga acccttccaa
    36301 aagatattct gccttttctt ctctgtattt ctttttatgt agttttttta attctttgat
    36361 tcttggattt tttgctgatt ggatctcttt cacattcctc acctcaactg attatatcat
    36421 ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattgat ttataaaaca
    36481 ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaat gtccaaggac gtgtccaggg
    36541 cgtgggattt cgctatatga ccaagatggt tgctgatcag ttaggcgtga ccggaacggt
    36601 aaaaaacgaa gacgacggct cagtcacaat cgaagcaatc ggaaatgacg acatcatcca
    36661 aaaatttatc gaagaagtca agaagtctcc gagcccaagt ggacgagtgc agtatgtgga
    36721 tgtccaagaa gatccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg
    36781 tttttgaagc gctttgcctc gagtattaag gagaaacatt ttcaaaatag cttctcatat
    36841 tttgaaatgt aatgacttaa taccgagagg cagctgataa aacaccgtca ataaggttgc
    36901 gaaaaagctg atctgcatca agcattaagg actggttgcg aaaaagtcgc ttagttttga
    36961 gtattaagga aaattttgtg aaaatggctt tccacatttt tgcataaatt tgtcttaata
    37021 ccaagaaact gacttttgaa caccgtcgat aaggttgttt ttgaggcgct ttgcctcaag
    37081 tattaaagac aggtagtaaa aaagctgatc tatcacttgc agtgagttca tttgactcct
    37141 ctatttcggg cagaataaaa aagtttttag ttttttaaat aagaaagaaa cacttggaat
    37201 cactcttttc tttccaagta tttctttctt gattttttgt cttttttcaa aatttttcgc
    37261 tacgaaaaag aaagaatatt tatatgaaat ttctatgaag ttgattgaat tccatgcgct
    37321 tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct atgaataaat
    37381 ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta tctggctgtg
    37441 tgcggatgga ttcaaacgga aaccctgaca cttcaggaat cgtttatcga gtactcgtcc
    37501 atcctatggg ccaagcgatt acatatctag ttgagaattt caattggtct tacggctggg
    37561 cagttattgc aatgactgtg atcgttcgta tcatcatctt gccactaggt atcagtcaat
    37621 cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag
    37681 cacaacaaaa gctaaaagct gcaacaacac gtgaagaaca aatggcagca caagcagaaa
    37741 tgcaacaagt atatcgcgaa aacggattga gtatgaccgg cggaatcggc tgtctgcctc
    37801 tattgatcca aatgccgatc ttctctgcct tgtattttac ggctcgctac acagaaggta
    37861 tccgtgaatc atctttttac ggcatcgatc taggctcacc aagtatggtg cttgttgcca
    37921 ttgcaggggt cgcttatctg cttcaaggat atatttcaac aatcggtatt cctgaagaac
    37981 agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga
    38041 tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc agctgtctgc
    38101 aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg gcagaagagt
    38161 tgaaacaaaa cccgccaaaa caagtcgtca ctccaagaaa agacgtgaca gaatcagcaa
    38221 agccagctga caacaaacaa gcaacaaaac aaatcaaaca accaaaaaac agtacaaaca
    38281 acaaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat aagagtgtaa
    38341 taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt gaaaaagaaa
    38401 tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg atcgctatct
    38461 aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa cgtagacagg
    38521 tgttgctgtt tcttttaata atgtacttaa ttccttaata ttttgttttc cttcttctaa
    38581 catccaacta ctcgcttttt tttcgccttc ggtaacaaaa ggtgccactc ccttcgccca
    38641 tgttgctcgc ccacataaga taccattgaa tgtcgaacct gattgttttg caaaacgtaa
    38701 tgtatcttga aatagcgtag cattgactcc cgcacttaaa aaaataaacg gttgttcagt
    38761 cactttcgat tgttcataga aatacgcgga tgcctctgct tgactataac aagtctcttg
    38821 cccatatcct tccacaaatc tcatattcac tggcacttct actttcaata catccacttg
    38881 ataacgttct tttgaaaatt ctcgcatggc ttcaataact ttatggggtt ttacttttgc
    38941 ataggcaaga gaagacggat cgccaatagt tgtatcataa gaaaggatct ctaaaaagaa
    39001 aggtatgcct tctgcttcgc attctgaacc aatccgttct ataactattt gtttttgcac
    39061 attaatctct gctggttcat ccacgtcata gtacaataaa aatttacaag catccgcacc
    39121 agcttctttt aaccttttca ccgaccaaat attcaataaa tcaggtaatc ttcccggcat
    39181 tttcgtatca tagccggttt tttcataagc tagcagcaag ccacattctt tctctcgaag
    39241 cgctgctgca ggcaggccgt attctggatc aagtaaaata gctgaggcat agggagtcag
    39301 gtattgagaa accatctgtt taaattggat gatgtcttct gatgtagctt ctttatattg
    39361 gttgatcatt ttcttcaaag caccacgctg gtcgattgct aacgcgctaa taattccagc
    39421 ttcatccgtc aatcgcttca aacgttcctg cttttctata ctcatcttct tcatatatct
    39481 tcacctattt ctttagttaa tattgtggct atttttgttt ttgattttgc ttttctcaac
    39541 tgttctgtaa cctcttttcg catcaatgat cgtgcaacga gagacaataa tttcaaatga
    39601 gtcgttcctg cttctgaatc tggaataaat aacgcaaaaa taaaatagta aatatctact
    39661 cgtgaaaacg ggtggttttt ggcagcccta gaaggcctca tcacttgctt atcctcttaa
    39721 gaggggcttt ttaagggacc accaacttgt accgaccccc aaaagttaga ccaaaaaatc
    39781 taacttttgg aggtcggtat tttttatggc taagtatact tttgaactta aattaaagat
    39841 cgttcatgat tatttggatg gcaaaggcgg aagtgattat ctggcaaaga a
//
LOCUS       DACEDD010000023        38903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEDD010000023 DACEDD010000000
VERSION     DACEDD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.023"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWO45_001321"
     CDS             complement(59..187)
                     /locus_tag="IWO45_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0332802.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWO45_001322"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWO45_001322"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0332803.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWO45_001323"
     CDS             complement(1674..2729)
                     /locus_tag="IWO45_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0332804.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWO45_001324"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWO45_001324"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0332805.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWO45_001325"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWO45_001325"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0332806.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWO45_001326"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWO45_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0332807.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWO45_001327"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWO45_001327"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0332808.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWO45_001328"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWO45_001328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0332809.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWO45_001329"
     CDS             10491..12050
                     /locus_tag="IWO45_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0332810.1"
                     /translation="MKKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWO45_001330"
     CDS             complement(12161..14092)
                     /locus_tag="IWO45_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0332811.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWO45_001331"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWO45_001331"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0332812.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWO45_001332"
     CDS             complement(15021..15773)
                     /locus_tag="IWO45_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0332813.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWO45_001333"
     CDS             complement(15932..16141)
                     /locus_tag="IWO45_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332814.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWO45_001334"
     CDS             complement(16152..17480)
                     /locus_tag="IWO45_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332815.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IWO45_001335"
     CDS             complement(17845..18432)
                     /locus_tag="IWO45_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0332816.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWO45_001336"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWO45_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0332817.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IWO45_001337"
     CDS             complement(20012..21301)
                     /locus_tag="IWO45_001337"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0332818.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IWO45_001338"
     CDS             complement(21455..22387)
                     /locus_tag="IWO45_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332819.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IWO45_001339"
     CDS             complement(22544..23203)
                     /locus_tag="IWO45_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0332820.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWO45_001340"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWO45_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0332821.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IWO45_001341"
     CDS             complement(24244..24534)
                     /locus_tag="IWO45_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332822.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IWO45_001342"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IWO45_001342"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0332823.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IWO45_001343"
     CDS             complement(26821..28176)
                     /locus_tag="IWO45_001343"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0332824.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IWO45_001344"
     CDS             complement(28200..29369)
                     /locus_tag="IWO45_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332825.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IWO45_001345"
     CDS             complement(29366..30253)
                     /locus_tag="IWO45_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0332826.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTRAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34134)
                     /gene="nifJ"
                     /locus_tag="IWO45_001346"
     CDS             complement(30442..34134)
                     /gene="nifJ"
                     /locus_tag="IWO45_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0332827.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34333..34854
                     /locus_tag="IWO45_001347"
     CDS             34333..34854
                     /locus_tag="IWO45_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0332828.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35553..36110)
                     /locus_tag="IWO45_001348"
     CDS             complement(35553..36110)
                     /locus_tag="IWO45_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332829.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(36289..36900)
                     /locus_tag="IWO45_001349"
     CDS             complement(36289..36900)
                     /locus_tag="IWO45_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332830.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(37013..37270)
                     /locus_tag="IWO45_001350"
     CDS             complement(37013..37270)
                     /locus_tag="IWO45_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332831.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(37867..38145)
                     /locus_tag="IWO45_001351"
     CDS             complement(37867..38145)
                     /locus_tag="IWO45_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332832.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(38179..38430)
                     /locus_tag="IWO45_001352"
     CDS             complement(38179..38430)
                     /locus_tag="IWO45_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332833.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        10635 a         8399 c         6813 g        13056 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atgaaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccgtcat tgatatttaa
    27541 cccgtctgga tttgtgccca ttgtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaactt catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatc
    29221 ggcacccccg taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatt agttctctac
    29461 gcaagattgc caaatattct tgctgggata agccagccat cagttcatta tctaacgtga
    29521 tgctgacgtc cccggtttct tcagagacaa cgatggtaat cgcgtcactg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attctttagg gatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc cccagtgata tctgcatcta
    29761 aagcaatccc tgtctcaata tactcatcta atccagtatg acgttcgata gtgatcaacg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgctct tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tatgctcgta tttttcttga gaaaaacgca taattttcaa
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct agcagctctt ctttcttctg cttcaaggtc
    30541 cgtcgttttt tcttttctag tcttaacggg tacatcgata gattcctctg gttcaagtcg
    30601 tttcttgatt ttttctaatt ctcccgtcag tgcagcatag ttgtaaaaac gtgttttagc
    30661 gtcgtggaca gtcttttcaa ataaagcttt tgcaagattt ggatttgcgt tatctagcga
    30721 ggaaaagcga acttgtttcc tcatgaaatc aggcatagca gagaaatcag gttttttgta
    30781 gtccaaagtc attgggttct tgtttttttc gaccattgta gggttatatc gatataatga
    30841 ccaataacca gaagaaacag cttcttttgc ttctttaagc gattctttca ttccgccaag
    30901 caagccatgt gtgatacaag gcgtataggc aataatcaga gaaggaccag gatacttttc
    30961 tgcttcttca aatgccttga tcgtttgcat ttgattggca cctgaagcaa tttgcgccac
    31021 ataaacattt ccatatgtca tcgccatcat tcctagatct ttctttgaag catactttcc
    31081 actagccgaa aatttagcga ttgccgaagc tggtgttgct tttgaggttt gaccgccagt
    31141 attggaatag acttcgttat ccaaaacaag gatattcacg tctgctccgc tggcaagtac
    31201 atgatcgatc ccgccaaaac caatgtcgta agcccatccg tctccaccaa tcatccattg
    31261 gctgtttttt acgaatagat cctttctctt gtatatatcc tccagttcag ggaaaattga
    31321 tcgttcgctt tcgatcgcag cttgcagttt tgctgctctt tgctgcgttc cttcactctc
    31381 aaacatgtgt actaaccagt cttccatcaa ttctcgtagt tcttctgata cttttggcaa
    31441 tacttctttg attgaacttg ccaaagcctc gcgacgagtc tgtgccgcta gaagcatacc
    31501 ataaccaaat tcagcattat cttccaataa agaatttgac catgcaggcc cttgaccaca
    31561 ttcattggtc gtataaggag aagcaggaga agcaccgccc caaatagaag aacagcctgt
    31621 cgcattcgcg atcatcattc gatcaccgaa catttgtgtc aataatttta cataaggtgt
    31681 ttcgccacag cctgagcaag cgcccgagaa ttcaagaagc ggttgattga attgtgtaga
    31741 caagacagta ttctttcctt tgactggatt tgctttttgt cttaatgtca ttgcaaaagc
    31801 ccaattcaca gcttgttctt tctgttcttc ataaggcttc atcactaaag ctttttcttt
    31861 tgcgggacaa gcctctacac acagaccaca acctgtacag tcttccactg aaacttggat
    31921 acggtatttt acgccgtctg cgcctcgcat ttctctcacg ataaatcctt ctggcgcttc
    31981 ttccatttcc tcttcatctg ctaaaaatgg acggatggca gcatgaggac aaacaaaggc
    32041 acattcatta cacatcgtac agcgatcact aatccactca ggtacttcta aagcgacgcc
    32101 ccgcttttcc atagaagccg ttcctaaagg catttcgcca gttgtcattc catttgctac
    32161 aagtgtacca acagagagtt gatttccttc ctgtgcattc actggttcaa gtatttcttg
    32221 tatataacca gtgttccttt tattgatttt ttcaggcatc tccaacgttc tccaatggtc
    32281 tgggatcgcg acttgatgaa gcagttcggt cgtccgatcg attgcttgta tgtttttttc
    32341 tacgatagcc attgatttat gtccatagct tttcagggct tcttctttta gcagcggaag
    32401 cacctcatca aaaggaatga tgtcagctaa tttaaaaaaa gccgtttcca ttgccgtatt
    32461 gatccggcgt cccaaaccga cttcttgggc caatctcatg gcattgatcg tatagaatct
    32521 aatgttattt tcagccaagt aacgttttaa ttttaagggc agatgcgttt ctaattgttc
    32581 gtcgctccaa agagtattca ataaaaaaat gccacctggc ttcaatcctt tgaccaagtc
    32641 atagctatgc aggtaagctg gtgtgtgaca ggcaacaagg tccgcatgct cgacaagata
    32701 cgctgaacgt atcggcgtat ctccaaaacg aagatgagag acagtcagac cgccagattt
    32761 tttcgaatca taataaaaat agccttgcgc atatttatcg gtgtgatctc caataatctt
    32821 gatagctgat ttatttgctc caactgttcc gtcagatcca aatccccaaa acttggcctg
    32881 gaatgtttgc ggttctgtca aatccagact ttcctttggt tccaaagatt gataagtcac
    32941 atcatccacg atccctatgg taaaccgttt tttgcgtatg cttgggtctt ttttcaattc
    33001 atcaaaaact gctgagattt ggtcgggagt cacatcttta gaccccaatc cgtagcgacc
    33061 accaataaca gccgggcgaa gttcagaatc atataacgca ctttgaacgt ctaataagag
    33121 aggttctcca cctgcccctg gttctttact tcgatcaaga acagcaattg tttttactgt
    33181 atgaggcaac ttttgcacga atgtttccac tggaaaagga cgataaagat ggatattcaa
    33241 aaaacctgtt ttccgtccgt ttgcattcaa gtaatcaatc gtttgttcaa tcgtctgtgc
    33301 aactgatccc atagaaacaa tgacttcttc cgcatcgtct gctccgtaat aagtcaccaa
    33361 gtcataagaa gttcctcgta atcggttgat ctctttcata tatttttgaa cgatttcagg
    33421 taatttatca taataggggt tgatcgtttc ccgttgttga aaatggatgt ctggattctg
    33481 gttcgtacca ctaacagtcg gatgattggg attcatccct cgtttacgaa atgaagctaa
    33541 tttctcctga ttgatcagag gagctaattc ttcatattcc aaaacttcga ttttttgaat
    33601 ctcgtgactc gtacggaatc catcaaagaa attcaaaaaa gggacttctc cttcaataga
    33661 agcaagatgg gcaacaggtg ctaaatccat cacttcttgt acactgcttt cagctaacat
    33721 agcaaatcct gtttgtcttg cagccatcac gtctccgtga tctccgaaaa tattcaacgc
    33781 atttgtcgtc actgcccgac tagcaacgtg gaaaacactt ggcagtagtt ccccagcaat
    33841 cttgtacata ttcggtatca tcaaaagcag cccttgtgaa gcggtatatg tagttgtcag
    33901 tgcaccagtt ttcaatgatc catggaccac accagctgct cctgcttcgg attgcatttc
    33961 tactactttt actggttgac cgaatacgtt ttcttttcct gctgctgacc attgatctac
    34021 taattcagcc atggtcgaac tgggtgttat tggatatatt gcagcaatct cggtaaatgc
    34081 ataagaaata tatgcggctg ccgtattgcc atccattgtt ttcatttttc gcatgattca
    34141 tcatccttgt tcatattctc tcttattatc tgttccatta taacgtaaaa catgccattt
    34201 ttttctttta tttataaaaa tattttacat gtatctattt gaattggtca tctaattttt
    34261 agacaaacag tctaaatcgt tgcctttttt tgtcagacat gtataaacta taagcgaaag
    34321 aggtgtttta acatggatat atcagtgatt gatgcaacga aaacaaatac acaaaaagga
    34381 atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa cttagcttgt
    34441 ccgtattgcc gacaatggtt tgaagaatct tatgagctat tagaagaagc agtccaaagc
    34501 gggcaattgc agcgagtgat caaattgttc gataaagaaa aagaaagctt acagcgaggg
    34561 aacgtgatgc accggtatct cacaatcagc gatggccaaa aagcgatcaa agagatcaaa
    34621 caaatattcg atactcaaga cgagtggaaa catctttctc tccaagaagt agcggatttt
    34681 gcagtagata aactaaagct gacagaacaa aaagacgaac agctgagcca agctgtcatc
    34741 aacgaagctg agcaagcaca tattcgattt gttccaactg tcatcttggg taaagaaatc
    34801 tttgatgaat ctatttcaat agaggaatta aaagaattga tccaaacaaa ataaaaaaat
    34861 aaggagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    34921 gcggaagcag aagcaactcc ttcttatttc ttggagttaa acgcttctgt cccagcctcc
    34981 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
    35041 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
    35101 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
    35161 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
    35221 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
    35281 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
    35341 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
    35401 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
    35461 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
    35521 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
    35581 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
    35641 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
    35701 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
    35761 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
    35821 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
    35881 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
    35941 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
    36001 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
    36061 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
    36121 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
    36181 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
    36241 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
    36301 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
    36361 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
    36421 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
    36481 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
    36541 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
    36601 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
    36661 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
    36721 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
    36781 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
    36841 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
    36901 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
    36961 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
    37021 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
    37081 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
    37141 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
    37201 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
    37261 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
    37321 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
    37381 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
    37441 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
    37501 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
    37561 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
    37621 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
    37681 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
    37741 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
    37801 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
    37861 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
    37921 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
    37981 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
    38041 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
    38101 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
    38161 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
    38221 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
    38281 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
    38341 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
    38401 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
    38461 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
    38521 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
    38581 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
    38641 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
    38701 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
    38761 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
    38821 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
    38881 gccagaataa accaattatt tgt
//
LOCUS       DACEDD010000024        38338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEDD010000024 DACEDD010000000
VERSION     DACEDD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.024"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IWO45_001353"
     CDS             161..391
                     /locus_tag="IWO45_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0332834.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IWO45_001354"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IWO45_001354"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0332835.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IWO45_001355"
     CDS             1802..1978
                     /locus_tag="IWO45_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0332836.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IWO45_001356"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IWO45_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0332837.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IWO45_001357"
     CDS             2593..3060
                     /locus_tag="IWO45_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0332838.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IWO45_001358"
     CDS             3145..4029
                     /locus_tag="IWO45_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0332839.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IWO45_001359"
     CDS             4146..4379
                     /locus_tag="IWO45_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0332840.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IWO45_001360"
     tRNA            4467..4547
                     /locus_tag="IWO45_001360"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IWO45_001361"
     tRNA            4554..4625
                     /locus_tag="IWO45_001361"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IWO45_001362"
     CDS             5140..5847
                     /locus_tag="IWO45_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0332841.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IWO45_001363"
     CDS             complement(5907..6551)
                     /locus_tag="IWO45_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0332842.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IWO45_001364"
     CDS             6678..6965
                     /locus_tag="IWO45_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332843.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IWO45_001365"
     CDS             7073..7393
                     /locus_tag="IWO45_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0332844.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IWO45_001366"
     CDS             7474..8787
                     /locus_tag="IWO45_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0332845.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IWO45_001367"
     CDS             8784..9503
                     /locus_tag="IWO45_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0332846.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IWO45_001368"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IWO45_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0332847.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="IWO45_001369"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="IWO45_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0332848.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IWO45_001370"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IWO45_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0332849.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IWO45_001371"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IWO45_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0332850.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IWO45_001372"
     CDS             14940..15833
                     /locus_tag="IWO45_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0332851.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IWO45_001373"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IWO45_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0332852.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IWO45_001374"
     CDS             17770..18627
                     /locus_tag="IWO45_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0332853.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IWO45_001375"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IWO45_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0332854.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IWO45_001376"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IWO45_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0332855.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IWO45_001377"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IWO45_001377"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332856.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IWO45_001378"
     CDS             21383..22141
                     /locus_tag="IWO45_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0332857.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IWO45_001379"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IWO45_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0332858.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="IWO45_001380"
     CDS             23069..24100
                     /locus_tag="IWO45_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0332859.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="IWO45_001381"
     CDS             24097..24864
                     /locus_tag="IWO45_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0332860.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="IWO45_001382"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="IWO45_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0332861.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26832..27149
                     /locus_tag="IWO45_001383"
     CDS             26832..27149
                     /locus_tag="IWO45_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0332862.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27149..27499
                     /locus_tag="IWO45_001384"
     CDS             27149..27499
                     /locus_tag="IWO45_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0332863.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="IWO45_001385"
     CDS             27666..28604
                     /locus_tag="IWO45_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0332864.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="IWO45_001386"
     CDS             28619..29449
                     /locus_tag="IWO45_001386"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0332865.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="IWO45_001387"
     CDS             29493..30518
                     /locus_tag="IWO45_001387"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0332866.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="IWO45_001388"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="IWO45_001388"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0332867.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="IWO45_001389"
     CDS             31596..32036
                     /locus_tag="IWO45_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0332868.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="IWO45_001390"
     CDS             32039..32641
                     /locus_tag="IWO45_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0332869.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32704..33807)
                     /locus_tag="IWO45_001391"
     CDS             complement(32704..33807)
                     /locus_tag="IWO45_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0332870.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34017..35018
                     /gene="ccpA"
                     /locus_tag="IWO45_001392"
     CDS             34017..35018
                     /gene="ccpA"
                     /locus_tag="IWO45_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0332871.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35373..35768
                     /locus_tag="IWO45_001393"
     CDS             35373..35768
                     /locus_tag="IWO45_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332872.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35768..36019
                     /locus_tag="IWO45_001394"
     CDS             35768..36019
                     /locus_tag="IWO45_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332873.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36062..36457
                     /locus_tag="IWO45_001395"
     CDS             36062..36457
                     /locus_tag="IWO45_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332874.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36454..37710
                     /locus_tag="IWO45_001396"
     CDS             36454..37710
                     /locus_tag="IWO45_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0332875.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37722..38045
                     /locus_tag="IWO45_001397"
     CDS             37722..38045
                     /locus_tag="IWO45_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332876.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38038..38304
                     /locus_tag="IWO45_001398"
     CDS             38038..38304
                     /locus_tag="IWO45_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332877.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12860 a         6514 c         8261 g        10703 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca caattcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcatgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 gatcgatgac tatgagcgta cacacgcttc tacgattgcc gttatgcaag ttcctcacga
    31021 tgaaacttca aaatacggaa tcatcaaccc aggggaagta ctggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tgggcgttac ttattgactc cagaaatctt tgatgtatta tcagaacaac aaccaggtgc
    31201 tggaaatgag atccaactaa ctgacgcaat cgatacgttg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttatga cgttggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctga cacatccaga agtaggggca cctttgcgtg aatacatcat
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc aatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata atcttttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgttttcat cgttcgcgta ttgttgcaag
    31681 tttccaaaac tttagaaaaa gtagaccaga ctgtcgcaga agcaaatacg acaattgaga
    31741 tcgtcacaca agacgtagat cttttatcta gacaagtaga aggcttgcta gtcaaaagta
    31801 atgagctgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcggaac ttaatcattc tagtaaaaac atcgtgacga
    31921 aagtcggcgg aatcggaaaa acgacagcta aagcgactgt tgccggaaaa gtcggtggaa
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc
    32101 cgcattattg ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc
    32161 agatgatttg atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga
    32221 gttaacaggt attgcaaaag aaaaagcgca aggtctttct caacaagcag gggatctagc
    32281 aggcaatgtt aagcagaagg taagtgaatc agcagaggaa gcaggaagtg aatcagatga
    32341 tatcttgtta aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc
    32401 agaagatctg aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga
    32461 tatcatgatc gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga
    32581 tgcaaaagaa gctaaaaaag aaatgaaaga tacaatgaca gattcgacta ccaatcacta
    32641 agattactca tttctagaat aaaaaaggtg agagcggacc agctctcacc ttttttattt
    32701 tcattactct aaaacaagta attcttttgg tgtagtcgtg aatggcacac aaccatcact
    32761 tgtcacatac acacaatctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32821 ttcaagagag aagcacatcc cttcttcaat cactaagtcg tttccttcta caagagaagg
    32881 gaattcgtgg acggttgttc cgattccgtg acctaaacgg tgattgaaat attcgccgta
    32941 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    33001 gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg atgttataga ttttttcttg
    33061 gaactcagtt ggtttttgat aagcaactgt acgagtagca tcactgcaat agccattcca
    33121 aataacgcca agatcgaata acactagttc atttggtgaa accttcgtgc ttcctggtgt
    33181 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33241 aacaccttgt ttttttaatt ggtattcgat ttctgcgata attgcttgtt cagttacgcc
    33301 ttcttttact gttttgaagc caatttcaaa ggcaacatcg gcccatttac cagcttcgat
    33361 cagtttgtct ttttcttcta ccgttttgat cagttgcaga cgctggataa tagaagttag
    33421 atcggtgtaa gaatctgtac ctgtcaagat atcagagata gcatcgaagc gatcgacagt
    33481 cagagcattc ttttcaatac ctacattacc aggttgccca tagcgtgcgc gaagctcttt
    33541 tccgatgatc tcccaaggat tttcgctgtc taagtagcca cgtacatcgt acgcccagct
    33601 gctgttttga gcatcttcaa cttccagagc aggagtgaaa aggaaaggat cttgttccag
    33661 agggataaaa agagctagta cacgctcgtg aggatcactt tcatatccgg aaaagtaagc
    33721 gatgtggcca gggtcgctga tataagccaa ttctacttgg tgttgtgcca tccattgacg
    33781 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33841 caataattgt accactgatt gattaaaaga ccaacgttct taaaactgaa aaaagtaaaa
    33901 attttcatga aaacagagat aaaacggttg caattgttga gattatttga tatcctattt
    33961 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    34021 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    34081 tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg
    34141 aagttatcga tcgcttggat tatcgtccaa atgcagtcgc acgtggcttg gcaagtaaga
    34201 aaacaacaac agttggggta atcattcctg atgtcagcaa tatgttcttt gcctctttag
    34261 cacgtggtat cgacgatgtg gcaacaatgt acaaatacaa tatcatctta gcaaactcag
    34321 atggcaacga tcaaaaagaa gtcaatgtat tgaataattt attggctaag caagtggacg
    34381 gcgtgatctt tatgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34441 aaacgcctgt tgtactagct ggttccattg atccagatga acaagtaggt agcgtaaaca
    34501 ttgattatgt gtcagcaaca aaagatgcag taaataaatt agcaaaaagc ggcaataaaa
    34561 aaatcgcttt tgtcagcggt gcgctgattg atcctatcaa tggtcaaaac cgtctaaaag
    34621 gctataaaga agcattaagc gaaaacaatc taccatatag cgaaggattg atttttgagg
    34681 cacaatacaa ctttaaagat ggtctgtctc tagttgaccg gatccataat agcggtgcaa
    34741 cagctgtcta tgtttcagac gatgaattag ccattggtat cttggatggt ttattagacc
    34801 gcggcgtaaa agtaccagaa gaattcgaaa tcatcacaag caacaattct ttactgacag
    34861 aagtagctcg tcctcgtttg acaagtatca cacaaccatt atacgattta ggagcggtat
    34921 ccatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34981 tgccttatgg aatcgaagaa aaaggatcaa ctaaataata aaagatcaat catttaaagg
    35041 ttgttccaaa agtggtcaac ttctggaaca acgtttatac gagaaagggg gttatgacaa
    35101 aattttgtca taaccccttt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa
    35161 accttacgtc catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact
    35221 atcaaaatta ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agataagtaa
    35281 ctctaaaaaa tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat
    35341 ccatcatata taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag
    35401 tcatcatttt gaccattctt actataggaa tcggagggaa attttacatg gacaaaagag
    35461 aaatgcaaag acagaaagat ttactcgcag tagaaaaaca gtccgtcaaa gttctgaaaa
    35521 atacctttgc ggatatcaag gaagtgaaga ttgaaaaatc tgctcgaaat gaaatgacgg
    35581 gttcataccg gatagttatt ctaatgacta ataaacaaga tcaatcgatt tatttttctt
    35641 atagtttttg gaaagaaaga aatgagatag gaagttacgg aatagttgat gaaaaaaagc
    35701 aaaaagaagg aaatacgctc aataaagtta aagtaactta ctctaatgga aatgaggaaa
    35761 gtatttaatg attactgaca aacaatatag tgaattaagt gatgctgttt actggctaga
    35821 tcctaaacat agagactatg tacctgaaat gcaagaaaat ttaagtttta aaattaacgg
    35881 cactatgtat aaaatcctaa aaatcaaaaa ctccttcgac ggcatgcagg cgatggctgt
    35941 agcgccaatc gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctgcaaa
    36001 ctttcgcgta cttaaataat gaaggtgtca atacttacat aaaaagaaag gaggcacgtt
    36061 cttgtcaagg aaaataagaa aactcagctt agccatttta ttaatcgtca tattagggat
    36121 cggagggaaa gtctatatgg acaaaagaga agcacaaagg cagaaagatt tactcgcagt
    36181 agaaaaacag tcagtcaaag ttctgaaaaa tacctttgcg gatatcaggg aagtgaaggt
    36241 agaagaattt aaaaaaaacc ctgttaccgg aagttatggt gcacttgtga caatgacaaa
    36301 taatgaaggg aaatctgttt attttgatta tagtttttgg aaggagagaa atgaacttgg
    36361 tggttttggc attgagaaca gagcggtaca aaagacagga gtcactataa aaagagtaaa
    36421 agtaattttt tctaacggac aggaggagtt agtatgacaa taaacgataa gcattacaat
    36481 gatattagtg agaaggttta ttggctagat cccaaatatc cccgctacaa tgagggatac
    36541 aaaaagaact ctgttaaaga atttgctggt atggaatttc aaatcctaca aatcaaagac
    36601 tcattggacg gcatgcaggc gatgtctgta gcgccaatca agaacggtaa agtggatctt
    36661 tctcaagtag tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg
    36721 gatgcaagaa gtatcatgaa tctctacggg aatagaccag gagcaagttt ttctgaaaaa
    36781 gcaacaacaa acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca
    36841 gcgattgcat ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc
    36901 ggccattcat taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat
    36961 gtcggcttca acgggccgga cccgtatgat ctcttatcac cagaagcaaa aaaatgggtg
    37021 gaagaaaatc ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt
    37081 ggtggtaatg gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg
    37141 cttgcttccc acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct
    37201 gaaaatgaaa acaatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg
    37261 ttttcctatc agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc
    37321 cagctgacag ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa
    37381 aagttatcgg caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga
    37441 atttttgagc tagagaaaaa atggcaagaa ggcttgcggc aagcacgtgt tgtagcccct
    37501 tcgttgactg aacaggaaat catagaagca ttagctagtg tgggcgccac aaaagaaaac
    37561 ttagtcgatt ctgcgaaaga agtctatctg ctacgaatca ataaggcaag acgtatgggg
    37621 gaggcctttg atacattggc gaaggagatc caagacaaaa tcaatgaaat cgtggcacga
    37681 gatcgagaat tagcacagca attcaattaa aggagaagta gatgaaacta gaagaattgg
    37741 aaagagatta tcgaagtgaa cgaagacaat tagaagctca agaagatgaa ttgatccact
    37801 ttaagaatca aggattacga ttgatggagc aagaggagca gcgactgcat catgtattaa
    37861 agaacttagc tttagataat gagcctctgt ttcaagcaag acgaagttat tcgaattatc
    37921 aggagcatta tcaacaagcc ctacaagtag aacgacgaaa actctatcag cagcaagatg
    37981 aactagaacg ttcctatcgt caaaaaatca gacaaataac tatggagaaa tcatcttatg
    38041 aatgaatttt taatgcaatt actaaaagaa ttagagaaaa atctacatcg atcaaatgta
    38101 cgttcagttg gtatggggat atctcagcaa atttgggcag aaaaacgatt gacagatcaa
    38161 aactaccaaa tgactgcaga agaagctgct caagaatttt ctaaccgaca tgtacacttg
    38221 aaaggatcag taaaagggca atttgcagaa aaactaactg aattattcca aagacaagaa
    38281 aagaaattaa aacagtttcg ttagtaaagt tatatagaaa aattttgtga aaatggtt
//
LOCUS       DACEDD010000025        36497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEDD010000025 DACEDD010000000
VERSION     DACEDD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.025"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            3..815
                     /locus_tag="IWO45_001399"
     CDS             3..815
                     /locus_tag="IWO45_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0332878.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="IWO45_001400"
     CDS             812..1612
                     /locus_tag="IWO45_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0332879.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="IWO45_001401"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="IWO45_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0332880.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="IWO45_001402"
     CDS             3109..4818
                     /locus_tag="IWO45_001402"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0332881.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9382
                     /locus_tag="IWO45_001403"
     CDS             5030..9382
                     /locus_tag="IWO45_001403"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0332882.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9526..9999
                     /gene="rimP"
                     /locus_tag="IWO45_001404"
     CDS             9526..9999
                     /gene="rimP"
                     /locus_tag="IWO45_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0332883.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10023..11198
                     /gene="nusA"
                     /locus_tag="IWO45_001405"
     CDS             10023..11198
                     /gene="nusA"
                     /locus_tag="IWO45_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0332884.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11220..11513
                     /locus_tag="IWO45_001406"
     CDS             11220..11513
                     /locus_tag="IWO45_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0332885.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11510..11821
                     /locus_tag="IWO45_001407"
     CDS             11510..11821
                     /locus_tag="IWO45_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0332886.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11834..14140
                     /gene="infB"
                     /locus_tag="IWO45_001408"
     CDS             11834..14140
                     /gene="infB"
                     /locus_tag="IWO45_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0332887.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14167..14514
                     /gene="rbfA"
                     /locus_tag="IWO45_001409"
     CDS             14167..14514
                     /gene="rbfA"
                     /locus_tag="IWO45_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0332888.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14622..14843)
                     /locus_tag="IWO45_001410"
     CDS             complement(14622..14843)
                     /locus_tag="IWO45_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0332889.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15291..17654
                     /locus_tag="IWO45_001411"
     CDS             15291..17654
                     /locus_tag="IWO45_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ0332890.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IWO45_001412"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IWO45_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0332891.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IWO45_001413"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IWO45_001413"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0332892.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IWO45_001414"
     CDS             20242..21027
                     /locus_tag="IWO45_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0332893.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21891
                     /locus_tag="IWO45_001415"
     CDS             21040..21891
                     /locus_tag="IWO45_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0332894.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22015..22467)
                     /locus_tag="IWO45_001416"
     CDS             complement(22015..22467)
                     /locus_tag="IWO45_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332895.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22932..23300)
                     /locus_tag="IWO45_001417"
     CDS             complement(22932..23300)
                     /locus_tag="IWO45_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0332896.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23450..23932
                     /locus_tag="IWO45_001418"
     CDS             23450..23932
                     /locus_tag="IWO45_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0332897.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23969..24979
                     /locus_tag="IWO45_001419"
     CDS             23969..24979
                     /locus_tag="IWO45_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0332898.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25094..25807)
                     /locus_tag="IWO45_001420"
     CDS             complement(25094..25807)
                     /locus_tag="IWO45_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332899.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25997..26587)
                     /locus_tag="IWO45_001421"
     CDS             complement(25997..26587)
                     /locus_tag="IWO45_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0332900.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27071..27298
                     /locus_tag="IWO45_001422"
     CDS             27071..27298
                     /locus_tag="IWO45_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0332901.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27314..28480
                     /locus_tag="IWO45_001423"
     CDS             27314..28480
                     /locus_tag="IWO45_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0332902.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28714..29757
                     /gene="hrcA"
                     /locus_tag="IWO45_001424"
     CDS             28714..29757
                     /gene="hrcA"
                     /locus_tag="IWO45_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0332903.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29851..30414
                     /gene="grpE"
                     /locus_tag="IWO45_001425"
     CDS             29851..30414
                     /gene="grpE"
                     /locus_tag="IWO45_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0332904.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30465..32294
                     /gene="dnaK"
                     /locus_tag="IWO45_001426"
     CDS             30465..32294
                     /gene="dnaK"
                     /locus_tag="IWO45_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0332905.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32445..33611
                     /gene="dnaJ"
                     /locus_tag="IWO45_001427"
     CDS             32445..33611
                     /gene="dnaJ"
                     /locus_tag="IWO45_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0332906.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33693..34358)
                     /locus_tag="IWO45_001428"
     CDS             complement(33693..34358)
                     /locus_tag="IWO45_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0332907.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34334..34507
                     /locus_tag="IWO45_001429"
     CDS             34334..34507
                     /locus_tag="IWO45_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0332908.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34735..34941)
                     /locus_tag="IWO45_001430"
     CDS             complement(34735..34941)
                     /locus_tag="IWO45_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332909.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(34967..35386)
                     /locus_tag="IWO45_001431"
     CDS             complement(34967..35386)
                     /locus_tag="IWO45_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0332910.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            35890..36243
                     /locus_tag="IWO45_001432"
     CDS             35890..36243
                     /locus_tag="IWO45_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0332911.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(36303..36470)
                     /locus_tag="IWO45_001433"
     CDS             complement(36303..36470)
                     /locus_tag="IWO45_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0332912.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT        12346 a         6316 c         7749 g        10086 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5701 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5761 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5821 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     5881 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     5941 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6001 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6061 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6121 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6181 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6241 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6301 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6361 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6421 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6481 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6541 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6601 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6661 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6721 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6781 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6841 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     6901 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     6961 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7021 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7081 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7141 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7201 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7261 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7321 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7381 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7441 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7501 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7561 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7621 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7681 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7741 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7801 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7861 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     7921 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     7981 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8041 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8101 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8161 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8221 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8281 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8341 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8401 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8461 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8521 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8581 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8641 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8701 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8761 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8821 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     8881 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     8941 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9001 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9061 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9121 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9181 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9241 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9301 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9361 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9421 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9481 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9541 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9601 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9661 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9721 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9781 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9841 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
     9901 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
     9961 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10021 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10081 aagaaatcgt gatcgatgca ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10141 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10201 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10261 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10321 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10381 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10441 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10501 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10561 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10621 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10681 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10741 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10801 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10861 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    10921 caaattcaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    10981 aacgcggaca aaatgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11041 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11101 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11161 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11221 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11281 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11341 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11401 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11461 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11521 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11581 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11641 aaccgatgca agtgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11701 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11761 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11821 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    11881 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    11941 tgcgattact agcagtgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12001 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12061 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12121 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12181 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12241 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12301 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12361 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12421 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12481 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12541 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12601 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tagtaacgat
    12661 tatgggacac gtagaccacg ggaaaacaac cttattggat acattgcgcc attcacgtgt
    12721 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12781 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12841 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    12901 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    12961 tgtaaataaa attgataaac caggtgcaaa tcctcagcat gttatgcaag aattaagtga
    13021 gtatgaatta attcctgaag catggggtgg agaaacgatt tttgttgaga tctcagcgaa
    13081 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13141 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13201 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcacg ttggtgaccc
    13261 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13321 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13381 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13441 acgcgcaaaa cgtgcattat tggaaaaccg tgctgccaca agccgtgtga ctttggacaa
    13501 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13561 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13621 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13681 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13741 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13801 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13861 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    13921 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    13981 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14041 aatgggttat gaatgtggtg cgatgattga aaaattcaat gacatcaaag tagatgacgt
    14101 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14161 atcattatgg ctaactaccg tgatcgccga gtcggtcaag aaatcttaaa agaagtcaat
    14221 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14281 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14341 tcagataaga aaaaagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14401 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14461 gttgtctatg ggaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14521 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14581 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14641 agaaaagtaa aaaattcgac tttctttttt ctatagtata taaatttttg catatagata
    14701 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14761 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    14821 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    14881 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    14941 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15001 gaattgctcg atccagcccc agttttccaa caatgtgtta actggcctag cgtcattcag
    15061 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15121 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15181 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15241 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15301 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15361 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa ccagaagatg
    15421 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15481 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatatcgaa gggccaggac
    15541 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15601 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caagcgattt tcatttcctg
    15661 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15721 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15781 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    15841 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    15901 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    15961 ttaacggact tggctgggag cctttcttta ttgaaggaaa tgatccagaa aaattaaatc
    16021 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat caaatccatc caaaaagaag
    16081 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16141 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16201 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16261 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16321 aagaaatcag agaaattgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16381 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16441 ttgaaaaact aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16501 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16561 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16621 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16681 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatacgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccactcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt
    21841 atcggattat tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga
    21901 tggtaaaatt tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa
    21961 aaaatagcgc tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga
    22021 tacaagggaa atatctgtct ggatcaataa tttttcattg atccattgac taaagtttac
    22081 gacataattg aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta
    22141 ttgtaaaaga cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc
    22201 tcggaattga ttgattgctg taaaatcacg atattgaagg ttgaaacgca tcgtgattgg
    22261 tgcatttagc atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag
    22321 gaactcaaaa agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag
    22381 ttttccttca tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa
    22441 ttcatttact gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac
    22501 ttagttgata tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt
    22561 aaaaatcaag tcagaaaatt ctaactttta tttctctaaa gaaattcgtt ctctcctaaa
    22621 gtgatattat atttaaatat acctgaaaat aatgggcagt attatgaaag atcactgata
    22681 ttttctcgta ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt
    22741 tactaagtag aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc
    22801 cttataaaaa tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat
    22861 aggattgaac aaaacctgta taaggtgatg aatagtttcc tcgccagtaa aaggtttatt
    22921 cgtataaagc tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa
    22981 atcaattgtt caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta
    23041 tccgtatata cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt
    23101 tccggtgcga tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg
    23161 tcagtaaaca taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt
    23221 atcttagcaa aaaggtcttt gatcttttgt tgatgttctt tttctttgat ttgattgatg
    23281 atcggctgaa atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca
    23341 gaaaaacaat agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata
    23401 tttatatgat aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt
    23461 ggcaaagaaa ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta
    23521 tacaaatgaa gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa
    23581 aagcttattc ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa
    23641 acaaacggat ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc
    23701 agaacgcatg gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga
    23761 aaaacagatt ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc
    23821 accaggtaga ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca
    23881 gctacttgag atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa
    23941 aatagtgcat acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc
    24001 aacgattggg gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga
    24061 atcagcttta aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg
    24121 gacgcaagat aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat
    24181 gatgcaagat gaaaatatcc aaatgatctt tccgacttgg ggaggagaat tattgattga
    24241 gattctcgat tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga
    24301 tattagtacg ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac
    24361 taaccttatt gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt
    24421 actgaaaaca agagaaggag aatcgattct acagcaatct tctgaaaagt atcaaaaagt
    24481 gtggccccat gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca
    24541 atcagtcagc ggagatgcag taaggatgaa gggacgatta ttaggtggtt gtattgatac
    24601 gatcaggcat ttaatcggta ctccttatgg agatgtccaa tcttttaaaa gaaagtataa
    24661 tgatggaaag ccgatcttgt ggtttctaga aaactgtgag ctatcgtcag ttgatttaag
    24721 aagatcactt gttcagatga aactggcagg ctggtttgat ggcagctcgg ggatcatgtt
    24781 cggtagaagt gcggcagagg atttgttaga ttataaaatg aaggatgtat acgaagatct
    24841 gtcaaaggaa ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca
    24901 actgacattg attaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt
    24961 tttgcaaacg tttatttaaa aaattttttc ctgccttgga gtctatcaaa gaccaataaa
    25021 tctaaataga aaagaaagtg aaagaagctg acaaaatgct gtcagcttct ttttgtataa
    25081 catattagaa agtctaatta tctataactg tcacatcgat taactgtttc acgtcattgg
    25141 gatttcctgt aggaatagct actacagatg cattatcaat gatttcttga gttgtttctc
    25201 taatgaacgc tggataagat gtttgatctg caggcatttg ccaatgtgaa ggaactggat
    25261 aactagtgat tgtcccgtca tggttacttg aatacactaa aactccctgg taactgtatt
    25321 tcccagtgag cataaccaca tctttcggat agactgcact ccctgcatca taaggggcga
    25381 taggtgttcc tgcaggataa tgaataacat gtaattcgaa atcacccgat gtatctctaa
    25441 aagtattacc tctaatagct agccaaacac gtgcgtactc aatttgtaga tcagaatatc
    25501 ctgctaacgg atctattgac tgagaagact gtgaggaggt tgtgtctatt gttgaagaag
    25561 aaatatttgt ctcaactgta ctactagatg tagaagaagt attgggctct gagactttac
    25621 tgcttgttga agatgtattc gtttcagtag aagctgattt ctgagtactt gatttttcag
    25681 ctgaactggt ttgtctgatt gaacttttgt ctgtggtcgc agatggatca ctattttctt
    25741 tactaggaga cgtacatcct acaaggaata aagagaccaa caataaaata aaacctaact
    25801 ttttcatata aaatacctca ttattttttt aataccagta tagcataatc ctgaattttt
    25861 gaatccgtaa aaccttgtct atgaatataa atagagtgta tcttctttga taaatgtcag
    25921 aaaaagtgtg acaaataatc atcagtaaaa ttacttgtca cactattgat aaggagtatt
    25981 taattgaaga gaatgattag gagaaaggat ctccgtactt tttatagtaa tcatagaatc
    26041 caagtttgta tagacgaatc gttttttctc ttatttcttc tgtttcaaaa tggactgctt
    26101 gaagaatcaa gtctgtgaat tctataggag ctgttccatt cgcagtaatc aaattacgat
    26161 ctttcacagc ttgttgttct ataaaatctt ctttattctg ataattttcc gattctaacc
    26221 aaggaaattg cgcatttccg gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat
    26281 aatcaactgc cccacaaata gcagctactg gtacgtctgt ttttaacaat ttatcgatta
    26341 tttgatataa atgatgattt tttatgtccc atgaattgcc accgatcata ataaaaaggt
    26401 caactttttc aggtatttgt tcgattaagt aatcgatttt tgtttttaat cctccaatgg
    26461 aagtgacctc tgattggata gaagcagttt tgttttgcca tttctcattt tggtttagtg
    26521 tgctagtaat ataagcacct tcccaatcgg catattcctc taatagaaac gtaatcgcag
    26581 ttttcatttt caagctctcc tttactatct ataacaagga caacagtttt aacctacaga
    26641 cattcattaa aattgggtag cgacagcgaa aacaacaatt cttaagtttt agacagagta
    26701 tacagatagt caacttaatt aaatacgaac taaataaaaa tgacaggtaa tcatttttgt
    26761 ttaccagcat tataacattt tgcatacatg gatgacaaaa gaaccaattc ccacttgctt
    26821 gagtacaata ttaaaagaaa ctggaaggca aaaagtaatt atagaaacga aaaataaatc
    26881 atatcgggca ctcagacatg aaaacaaccc tagagatata taactaggta accagtttaa
    26941 tgaaagaaaa aatcatccaa gaagtcgatt cttggatttt ttagtggatt tgttcgccaa
    27001 atacgtttag tatgcgtata tgagaggttg taagcaaagc gttgtggtct gagcacttaa
    27061 ggaggaatct gtgaaaatag cttttcatat ttttgcagat tttgacttaa tgccgaaggt
    27121 cagctttgcg attaccgttt atcgagaggc tgtaaaagca ccgtgtagct ccgatgacaa
    27181 taagcaagca tttttagagc taggtttcct tgattctaaa tatgcggctt atttcaatga
    27241 gttggtgctt gaacgcgatt ttaagaaggt cgaaaacagg cacgactttt cgacgtaagg
    27301 agaaagaatt tcaatgatag aaacaataaa tcccgcagaa aacaaggttt ctgcgtacat
    27361 tcatatccca ttttgcgagc atatctgcta ttactgtgat tttaataaag tttttttaga
    27421 agggcagcca gtagatgagt atgtggaaat gctgctgaat gaaatgaaaa taatgctaga
    27481 aagtcatccg acagcgaatt tagaaacact ttacgtaggg ggcgggacac caacttcttt
    27541 atctgctaaa caacttgatc gactgttatc aggtgctaga gagatcctgc cttttcaaaa
    27601 tggtaatgag tttactgtag aagcgaatcc aggtgatttg acccgtgaaa aacttcaagt
    27661 aatgaaaaac tatggcgtca atcgtttatc catgggagtg cagacatttg ataatcgctt
    27721 attgaaaaaa attggacgta aacacaccgc agaagatgtg tatcaaacta tgcaattttt
    27781 agaagcagaa aattttacca atgtaagcat cgatttgatt tatgcattac cgggacaaac
    27841 actagaagga tatctggata ctttggaaaa agctttggct ttggatctgc ctcactattc
    27901 tttgtactca ttgatcctcg aaaacaaaac aatgttcatg aactgggtac gacaaggaag
    27961 actcgaactg cctgatcagg aaacggaaac cagaatgttt gaagagacga ttgaagcaat
    28021 ggaaaagcat ggacgtcacc aatatgaaat aagtaacttt gggctctccg gacatgaatc
    28081 tcaacataac ctcatgtact ggaacaatga ccactatttt gggtttggag ctggtgctag
    28141 cggttatctt ggagatattc gttaccgcaa caaaggacca atccaacact atttacgccc
    28201 attacgagca aatgaacgcc cggtattgga agaagaaaaa ttgacacgaa aaaatcaaat
    28261 agaagaagaa atgttcttag gactaagaaa aaaaacaggg attactaaaa aacatttcta
    28321 tgatcgctat caaaaaacaa ttgaatcggt gtacggtgat gtgctaaggg aattagagca
    28381 agaagggtta cttgttaatg aagaagaccg tgtttatttg acaccaagag ggaccttttt
    28441 ggggaacaat gtgtttgagc gctttttgtt tgaaaaatag tttaaagaat atttgttttt
    28501 tatctgaatt taaataaaaa aagttcaaaa atacaataaa ggactattga aacgttgata
    28561 taactggatt tacatcaatt atttagaatt agcactcttg tgtttagagt gctaaaaaat
    28621 ggagtctttc tcttgacagg agaagggctt cttgctatat ttataagtgg attagcactt
    28681 agaatatatg agtgctaatg agaggtgagg aagatgttaa caaagcgaca aaacgacatt
    28741 ttacgtctga tcatccaaca ctatacgagt agtggcgtgc ccgttggttc caaaacattg
    28801 atgtctgagg gagtcaaggc cagctcagca acgattcgca atgatatgaa agcattagaa
    28861 gatgaaggat tgctgttgaa gactcactct tcttctgggc ggattccatc tgctttaggc
    28921 tatcgttatt acgtggatca tttgttaaaa cctgctcgcg tagcagaaga tgatttgctg
    28981 ctgattcgtc agtctctagg cagagaattt catgaaatca atgagattat taagcaatct
    29041 gctgagatat tatctgagct gacaagctat acgacttttt cattgggacc tgaaataaaa
    29101 gaccgccgac tgactggctt tcgtatcgta cccttgaata atcgacagat tattgctatc
    29161 gtcgtaacag atatggggaa tgtagaaagc caagtgttca ctcttcccga atatatggga
    29221 agtcaggaac tagaaaaaat ggtacagatt gtaaacgacc gtctagtcgg agatcctctg
    29281 atgacggtgt accataaact tcgtacggag attccgatga ttcttcataa atatttccaa
    29341 acgacagaag ggatgtctca gttatttgac accgttcttg gtcatgtttt tgaagataag
    29401 gtttatgtca gtggacaaat gaatctcttg gattatgagc cccgtcaaga tcttgatcag
    29461 ttcaaatcga tgttttcttt tatgaaagac tcagacggac ttacacaaat gatcactccg
    29521 atggatagca atattcatat caagattggt gctgaattag gcaatgattt attgcagaat
    29581 atgagtatga ttcaagcaag ttacgagata gatggtcatg gaagaggtac aatagccttg
    29641 ttagggccta ctagtatgcc ttattctcgg atgttcggtc tagtcgatgt ttatcgaaga
    29701 gaactagcag ctaaacttgc tgattactac cgttcgcttg acttgtcaaa ctcgtaatgt
    29761 gaataacgaa gctatttgca gctgatcgtt ctctctttct tagtaagagc aatcaaacag
    29821 ctgactgaat aatgaaagga agactgtatc gtgaaagaaa atcaagaaga actagataaa
    29881 gaaatgacag atgctcagcc agaaccggaa atcgatgtcg aagctgcaga agattcgggt
    29941 atttcagaag ctgaagcgga agaattcgaa acagcgaaat taaaagctga gttagaagaa
    30001 atggaagata aatacttgcg ggctcgtgct gaaatcgcaa atatggcaaa tcgaggaaaa
    30061 aatgagcgtg agcaattaca aaaataccgt tcacaagatt tagccaaaaa attacttcca
    30121 tcaattgaca acctggaacg tgcattagcc acagaagtgt cagatgatca aggggcaggt
    30181 ttgaaaaaag gcgtggaaat ggtcttggaa agtttgcgta acgcactgga agaagaagga
    30241 attgaaaaaa tccctgctaa aggagaagcc tttgatccta atcttcatca agcagtacaa
    30301 actgttccag cgactgaaga tacaccagcc gatacgattg tagaagtact acaagaaggc
    30361 tacaaattac atgatcgcgt gttaagacca accatggtca ttgttgcaca ataattgtgc
    30421 ggatataaaa cttacacttt caattaaaga aggagacgtt aattatgagt aaaattatcg
    30481 gtattgactt aggtacgact aactctgcag tagcagtatt ggaaggaaac gaagcaaaaa
    30541 tcatcgctaa cccagaagga aaccgtacaa caccttctgt tgtatcattc aaaaatggag
    30601 aaatccaagt aggggaagtt gcaaaacgtc aagctgtgac aaacccaaat acgatttcat
    30661 caatcaaacg ccatatcggt gaagcaggtt acaaagtaga tgtagaaggt aaatcatata
    30721 caccacaaga aatctcagca atgatccttc aatatatcaa aggattcgca gaagactatt
    30781 taggtgaaaa agtcgaaaaa gcagttatca ctgtgccagc ttactttaat gatgcacaac
    30841 gtcaagcaac aaaagatgct ggtaagatcg ctggtctaga agtagaacgt atcgtcaatg
    30901 aaccaactgc agctgctctt gcctatggtt tagataaaac agatcgcgac gaaaaaatct
    30961 tagtattcga ccttggtggc ggtacttttg acgtatctat ccttgaacta ggagacggtg
    31021 tctttgatgt attgtcaaca gctggcgaca accatctagg tggggacgac tttgataaca
    31081 aaatcatcga ctacatggta gcagaattca aaaaagaaaa cagcattgac ttgtctcaag
    31141 acaaaatggc tttacaacgc ttgaaagacg ctgctgaaaa agcgaaaaaa gatttatctg
    31201 gcgtatcaag cacacaaatc agcttgccat ttatcacagc aggagaatct ggaccattgc
    31261 atttggaaat gacattgaca cgtgcaaaat ttgatgaatt aacagctgac ttagtagaac
    31321 gtacaaaagt accagtacgt caagcgctaa aagatgcagg tttatcacaa tcagaaatcg
    31381 acgaagttat tttagtcggt ggatctactc gtatccctgc agttgtagaa gcagtacgta
    31441 aagaaacagg aaaagaacca aacaaatcag ttaaccctga tgaagtagtt gctatgggtg
    31501 ctgctatcca aggtggcgtg atcactggtg atgtgaaaga tgttgtttta cttgacgtta
    31561 caccattgtc attaggtatc gaaacaatgg gtggagtgtt cactaaattg attgaccgca
    31621 atacaacgat tccaacaagt aaatcacaag tattctcaac tgcggcagat aatcaaccag
    31681 cagtagacat ccatgtatta caaggtgaac gcccaatggc tgctgacaac aaaactctag
    31741 gaagattcca attgacagat attccagcag ctcctcgtgg tattccacaa atcgaagtaa
    31801 cattcgatat tgataaaaac gggattgtaa atgtatctgc gaaagatcta ggtacacaaa
    31861 aagaacaaaa aatcacgatc aaatcttctt caggtctaac agacgaagaa atcgaacgta
    31921 tggtaaaaga tgcagaagca aatgcagaag cagataaagc tcgtaaagag gaagttgacc
    31981 tacgtaatga tgtagatgca ttgctattct ctgtggacaa aacattgaaa gaattagaag
    32041 gcaaagtgga cgaagaagaa gtgaaaaaag cagaaacagc tcgcgatgaa ttgaaagccg
    32101 ctgttgaagc aaacaacatc gaagaaatga aaactaaacg cgatgcattg aacgaaatcg
    32161 ttcaaaactt gactgttaaa ctatacgaac aagcggcaca acaacaagct caagaaaacc
    32221 cagaagcagc tcaaggcgga gcagatgatg tcgttgatgc tgattttgaa gaagtcgatg
    32281 gcgacgataa ataatcagtt cactgaattt cgagagaagg ccaaagttgc tactttggct
    32341 ttttcttttg aaaaatagtt tgaagtatcg gtttttgttt caaacaaaat atgatatgat
    32401 aacaagcgac gtgaaaatga tgagaaccaa taggaggaaa gcctatggca acaaaacgtg
    32461 attattatga agtcttaggg ctctcaaaag gagcatcaga agatgaaata aagaaagctt
    32521 atcgtaagct ttcgaaaaaa taccatcctg acatcaataa agaacctgat gcggaagaaa
    32581 aattcaaaga agtatcagaa gcatatgaga ttttaagtga cccgcaaaaa cgcgctgcat
    32641 atgaccaata cgggcatgca ggcacagatc caaattacgg tgctggtgga gctggtggcg
    32701 gatttggtgg attcggcggc ttctctggtg gcggcttcgg tggttttgaa gatatcttcg
    32761 actctttctt tggaggaggc ggtcggtcag tcgatccaaa tgcgcctcgt caaggtgctg
    32821 atttgcaata tacggtcgac ttgtcttttg aagaagcgat ttttggtgta gaaaaagaaa
    32881 tcaagtataa tcgggaagaa atctgtcata cttgtggtgg gaacggtgca aaaccaggca
    32941 cacaaccaac tacttgtcat aaatgtcatg gtgcaggtac gattaatgta gaacgccaaa
    33001 caccacttgg acgagtgatg agccgtcaaa cttgtgatgt ttgtcatggg acagggaaag
    33061 aaatcaaaga accttgtcca acctgtcatg gctcaggaca tgagaagaaa gcacattcag
    33121 ttaaagtgaa tgtaccagct ggtgtagaag aaggacaaca aatgcgttta gctggtcaag
    33181 gagaagctgg agaaaacggc ggaccatttg gtgatttgta tgtggtcttc cgtgtagaag
    33241 aaagtgatat ctttgatcgt gaaggttctg aaatttacta tgaattgcct ttaagttttg
    33301 tacaagcagc tttgggggat gaagtccaag taccaactgt ccatggagat gtgaaattga
    33361 agatccctgc tggaacacag acaggtacga acttccgttt acgtggaaaa ggcgctccga
    33421 agttacgtgg tggtgcaaca ggcgaccaac atgtcaaagt aaaactgatc acaccgaaaa
    33481 atctaaatga acaacaaaga gaagcattac gtgcattcgc tgaagctggc ggcatcaaag
    33541 ttgaagaaca gcaagaagac ggtttcttcg ataagtttaa agatgctttt ggcgggaaaa
    33601 agcggaagta gtttttctag taaaaacgct aagtcattaa taattaaaaa ccagacgaac
    33661 aatcatatgt tcgcctggtt tttttgtagt tgtcatgttt gtaccaacaa aaaagccagc
    33721 tttagaatat gataatcgtc atagtttcta ggggtataac cagtgagaga atggaacttg
    33781 ttcaatcgat attgcaaggt gtttttatga ataaataatt cttctgagca atttttgatg
    33841 cttccgttat ttttactgaa gcatgccaaa gtcttcttga acaaatgtac ttcttcttct
    33901 gtcaggcttt tcaaaatacg ttttacatat aatgttcgtt gatctgacgg gattgctggt
    33961 aaaacgatcc cgaggtctaa atcttcaaaa aagcggatct cgttcacaga ttgatggaaa
    34021 gctatccatt cgcaaacttg tttggcatga aggaaagatt gcggtgtctc ttgctctgta
    34081 taacaaagcg acccaattcc aaagtttagc tgtaactgat acattttatt aaactttgtc
    34141 tggattttat caagcaatga tagcaatgag gctctggaaa tccgattgac taggataatg
    34201 atttgttgtc tatgtacagt gagcaaagcc tgtccgtaat aagagaagac cctttcgagc
    34261 aactccaata aaggattgat ccgttcttga cacaattgct gattgtcaga aagttgtccc
    34321 acaactacat aatatggaga agaaaaatca agctcaatta agtgagggtg gtatcgacta
    34381 tgtacttgat atcatcaatg aaccgatggt actttcttat gcgatggaac aggtagagcg
    34441 attgcttgtt tgtgcaggaa aacaagcgat gcggctgata cgattaggtg agaaaataag
    34501 aagataaaaa tacacggtcc cttcatggcg accgtgcatt tttggttttt attaaatttt
    34561 atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg aagtatagaa
    34621 aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg accgtaaaac
    34681 tactaaggga gataacacga aactgtgtat ctcccgattt ttttatacag ttgattactt
    34741 cactccgatt tcaaatttca atattgcttt catcatgtct gtatgtattt ctccttttag
    34801 ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag gacgcaagca
    34861 taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca tttcatctcc
    34921 gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac ttcctttcgc
    34981 tcaaaagcat ttttaacttc cttagtcctc tagttcttct actgcaaata gcagaacgtg
    35041 atacatggta cagttctgaa atttctctgt cgttcattcc ttgaaaataa cgtaataaaa
    35101 tcgcactacg ctgtttttct gatagtctat gtaaaactgt accaagtgtt tcatcagata
    35161 cataaattgt aaagtgcaag actttaaatg atacacaatc ataaaaacag gtatcaatcg
    35221 ttccacgttc aacttgtttc gtttcggcta tttcacaaaa caatacttca cgtttagaat
    35281 gtcttgcaag ctgtctgttt cgacttttca ctttgttctt aatgacaatc agcattaaag
    35341 aattgaactg taatctgacg atatgctcga aagaagatgt cctcataatc tcaccccctt
    35401 tctgtctagg aaaggaagtg agccttgctc gtttatctcc tttcactctt agtcccaatg
    35461 tgaaaggggg atttgttgca ttactgataa ataaactttg taaaaaagtt ctgaatagcc
    35521 aaaaaagcat ataaacagat ttatttctct gtttacatgc ttctgttatt ctatctatat
    35581 gatttataaa accacattgg tggacgtact tatctattgc agatagacga ctttttttga
    35641 caagaaccca atgtaaggaa atttattgta tatgatgtac ttcatggcga cgttgacctc
    35701 caacaaaccg ccatttggaa gtaatataca atattttaac agcgtaaata gcactaccat
    35761 ataacggttt tttttattgg cgtttagtag tgctttttat taaatataaa cctataaacc
    35821 atataacacg tttttctata cctgttttta attcagtagg aacaataaaa tgtatagagg
    35881 tggtctacta tgcgtaaaaa agaagataaa tatgatttta gagcctttgg tttagccatt
    35941 aaagaagctc gattgaaacg aggtttaact cgtgaacaag tgggagcatt gattgaaatt
    36001 gacccacggt acttaactaa tattgaaaat aaagggcaac accccagcat acaagttctt
    36061 tatgaccttg tatcgttact tcatgtttcc gttgatgaat ttttcttacc tgctaataac
    36121 ttggtaaaaa gcacccgacg attacagata gagaaataca tggatagctt tacagacaaa
    36181 gaactatcct taatggaatc tttagccagc ggtatcaacg aagcaagaaa catcgaagac
    36241 taattaaaag aatccataca taacggaaag agccgataaa atgagattgt attaatctca
    36301 ttttatcggc tctgcgtctt tgcgtctggc tctgtaatca cagttacttt gaactgcttt
    36361 atttcaatta aattttcttg tctgcatttc ggacaataga gggggaattt ttttaattca
    36421 gtatcttccc ttatctttaa tcgtgtttta tttccacata caggacacaa tatccacttg
    36481 tagtttataa taactat
//
LOCUS       DACEDD010000026        35438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEDD010000026 DACEDD010000000
VERSION     DACEDD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.026"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1152
                     /locus_tag="IWO45_001434"
     CDS             <1..1152
                     /locus_tag="IWO45_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332913.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IWO45_001435"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IWO45_001435"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0332914.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IWO45_001436"
     CDS             2728..3003
                     /locus_tag="IWO45_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0332915.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IWO45_001437"
     CDS             3009..3776
                     /locus_tag="IWO45_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0332916.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IWO45_001438"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IWO45_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0332917.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IWO45_001439"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IWO45_001439"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0332918.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IWO45_001440"
     CDS             7920..8222
                     /locus_tag="IWO45_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0332919.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IWO45_001441"
     CDS             8225..9388
                     /locus_tag="IWO45_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0332920.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IWO45_001442"
     CDS             9414..12842
                     /locus_tag="IWO45_001442"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0332921.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IWO45_001443"
     CDS             12863..13987
                     /locus_tag="IWO45_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332922.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IWO45_001444"
     CDS             13977..14414
                     /locus_tag="IWO45_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0332923.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IWO45_001445"
     CDS             14398..14736
                     /locus_tag="IWO45_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0332924.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IWO45_001446"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IWO45_001446"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0332925.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IWO45_001447"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IWO45_001447"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0332926.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IWO45_001448"
     CDS             15893..16936
                     /locus_tag="IWO45_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0332927.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IWO45_001449"
     CDS             17095..17823
                     /locus_tag="IWO45_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0332928.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IWO45_001450"
     CDS             17939..18184
                     /locus_tag="IWO45_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0332929.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IWO45_001451"
     CDS             18181..18399
                     /locus_tag="IWO45_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0332930.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IWO45_001452"
     CDS             18535..19074
                     /locus_tag="IWO45_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0332931.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IWO45_001453"
     CDS             19067..21340
                     /locus_tag="IWO45_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0332932.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IWO45_001454"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IWO45_001454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0332933.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IWO45_001455"
     CDS             complement(22513..22767)
                     /locus_tag="IWO45_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0332934.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IWO45_001456"
     CDS             complement(23167..23436)
                     /locus_tag="IWO45_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332935.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IWO45_001457"
     CDS             complement(23906..24361)
                     /locus_tag="IWO45_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0332936.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IWO45_001458"
     CDS             complement(24499..26427)
                     /locus_tag="IWO45_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0332937.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLGFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IWO45_001459"
     CDS             complement(26624..27457)
                     /locus_tag="IWO45_001459"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0332938.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IWO45_001460"
     CDS             complement(27649..28353)
                     /locus_tag="IWO45_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0332939.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IWO45_001461"
     CDS             complement(28376..29002)
                     /locus_tag="IWO45_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0332940.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IWO45_001462"
     CDS             29328..31703
                     /locus_tag="IWO45_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0332941.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IWO45_001463"
     CDS             complement(31727..32218)
                     /locus_tag="IWO45_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0332942.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IWO45_001464"
     CDS             32405..33325
                     /locus_tag="IWO45_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0332943.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IWO45_001465"
     CDS             33479..33823
                     /locus_tag="IWO45_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0332944.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IWO45_001466"
     CDS             complement(34013..34513)
                     /locus_tag="IWO45_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0332945.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IWO45_001467"
     tRNA            35041..35124
                     /locus_tag="IWO45_001467"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11775 a         6171 c         7295 g        10197 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa atcccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEDD010000027        34636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEDD010000027 DACEDD010000000
VERSION     DACEDD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.027"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWO45_001468"
     CDS             591..1280
                     /locus_tag="IWO45_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0332946.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWO45_001469"
     CDS             1267..2475
                     /locus_tag="IWO45_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0332947.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWO45_001470"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWO45_001470"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0332948.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO45_001471"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWO45_001471"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0332949.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWO45_001472"
     CDS             complement(6578..7273)
                     /locus_tag="IWO45_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0332950.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO45_001473"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWO45_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0332951.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWO45_001474"
     CDS             complement(8472..8663)
                     /locus_tag="IWO45_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0332952.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWO45_001475"
     CDS             complement(8684..9574)
                     /locus_tag="IWO45_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0332953.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWO45_001476"
     CDS             complement(9561..10328)
                     /locus_tag="IWO45_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0332954.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO45_001477"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWO45_001477"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0332955.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO45_001478"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWO45_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0332956.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO45_001479"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWO45_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0332957.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWO45_001480"
     CDS             14944..15282
                     /locus_tag="IWO45_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332958.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWO45_001481"
     CDS             complement(15450..16208)
                     /locus_tag="IWO45_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0332959.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWO45_001482"
     CDS             complement(16226..17044)
                     /locus_tag="IWO45_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0332960.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO45_001483"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWO45_001483"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0332961.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO45_001484"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWO45_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0332962.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO45_001485"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWO45_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0332963.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO45_001486"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWO45_001486"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0332964.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWO45_001487"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWO45_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0332965.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWO45_001488"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWO45_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0332966.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWO45_001489"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWO45_001489"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0332967.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWO45_001490"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWO45_001490"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0332968.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWO45_001491"
     CDS             27086..27385
                     /locus_tag="IWO45_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0332969.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWO45_001492"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWO45_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0332970.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWO45_001493"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWO45_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0332971.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWO45_001494"
     CDS             28352..30325
                     /locus_tag="IWO45_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0332972.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWO45_001495"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWO45_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0332973.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWO45_001496"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWO45_001496"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0332974.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWO45_001497"
     CDS             33204..34496
                     /locus_tag="IWO45_001497"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0332975.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6732 c         6844 g        10506 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgttttcc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DACEDD010000028        34347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEDD010000028 DACEDD010000000
VERSION     DACEDD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.028"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWO45_001498"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWO45_001498"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ0332976.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWO45_001499"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWO45_001499"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ0332977.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWO45_001500"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWO45_001500"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ0332978.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IWO45_001501"
     CDS             complement(4028..5044)
                     /locus_tag="IWO45_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0332979.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="IWO45_001502"
     CDS             complement(5146..5343)
                     /locus_tag="IWO45_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0332980.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="IWO45_001503"
     CDS             complement(5315..6634)
                     /locus_tag="IWO45_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAQ0332981.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="IWO45_001504"
     CDS             complement(7375..8355)
                     /locus_tag="IWO45_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0332982.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="IWO45_001505"
     CDS             complement(8368..9246)
                     /locus_tag="IWO45_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0332983.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="IWO45_001506"
     CDS             complement(9262..10062)
                     /locus_tag="IWO45_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAQ0332984.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            11454..11780
                     /locus_tag="IWO45_001507"
     CDS             11454..11780
                     /locus_tag="IWO45_001507"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0332985.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11788..12696
                     /locus_tag="IWO45_001508"
     CDS             11788..12696
                     /locus_tag="IWO45_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0332986.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13325..13576
                     /locus_tag="IWO45_001509"
     CDS             13325..13576
                     /locus_tag="IWO45_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ0332987.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14118..14276
                     /locus_tag="IWO45_001510"
     CDS             14118..14276
                     /locus_tag="IWO45_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0332988.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14348..15574
                     /locus_tag="IWO45_001511"
     CDS             14348..15574
                     /locus_tag="IWO45_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0332989.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="IWO45_001512"
     CDS             complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="IWO45_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0332990.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="IWO45_001513"
     CDS             complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="IWO45_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0332991.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(17033..17500)
                     /locus_tag="IWO45_001514"
     CDS             complement(17033..17500)
                     /locus_tag="IWO45_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0332992.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(17620..19158)
                     /locus_tag="IWO45_001515"
     CDS             complement(17620..19158)
                     /locus_tag="IWO45_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0332993.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            19238..19924
                     /locus_tag="IWO45_001516"
     CDS             19238..19924
                     /locus_tag="IWO45_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0332994.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="IWO45_001517"
     CDS             complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="IWO45_001517"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0332995.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(20736..21413)
                     /gene="pgmB"
                     /locus_tag="IWO45_001518"
     CDS             complement(20736..21413)
                     /gene="pgmB"
                     /locus_tag="IWO45_001518"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0332996.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(21410..22540)
                     /locus_tag="IWO45_001519"
     CDS             complement(21410..22540)
                     /locus_tag="IWO45_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0332997.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(22540..24723)
                     /locus_tag="IWO45_001520"
     CDS             complement(22540..24723)
                     /locus_tag="IWO45_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0332998.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(24720..25745)
                     /locus_tag="IWO45_001521"
     CDS             complement(24720..25745)
                     /locus_tag="IWO45_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0332999.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(25790..26617)
                     /locus_tag="IWO45_001522"
     CDS             complement(25790..26617)
                     /locus_tag="IWO45_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0333000.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(26614..27681)
                     /locus_tag="IWO45_001523"
     CDS             complement(26614..27681)
                     /locus_tag="IWO45_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0333001.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(27707..28543)
                     /locus_tag="IWO45_001524"
     CDS             complement(27707..28543)
                     /locus_tag="IWO45_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0333002.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(28540..29409)
                     /locus_tag="IWO45_001525"
     CDS             complement(28540..29409)
                     /locus_tag="IWO45_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0333003.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(29479..30762)
                     /locus_tag="IWO45_001526"
     CDS             complement(29479..30762)
                     /locus_tag="IWO45_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0333004.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(30776..32461)
                     /locus_tag="IWO45_001527"
     CDS             complement(30776..32461)
                     /locus_tag="IWO45_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0333005.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            32736..33761
                     /locus_tag="IWO45_001528"
     CDS             32736..33761
                     /locus_tag="IWO45_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0333006.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9802 a         7370 c         6045 g        11130 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caagagttag
    10141 ttcaataaca cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt
    10201 ataatatgaa tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag
    10261 aaaaacaacc tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt
    10321 tccagtttta ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat
    10381 tctcctcttt atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa
    10441 aagcatttct aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt
    10501 taactatttg atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga
    10561 cctttcggga gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt
    10621 tcgtatacct aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa
    10681 aacaaggggc ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc
    10741 cttgttttgt cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac
    10801 gaaaatgacc tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct
    10861 cccaaaatgt cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt
    10921 aagtattaaa taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca
    10981 aaaataaact gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta
    11041 atttatcgat tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca
    11101 gcgaatccag cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc
    11161 ttcctacgaa aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta
    11221 ttccgtgtta ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg
    11281 gcggggctgt atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat
    11341 caatgaatgg cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc
    11401 gccggatgac cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca
    11461 aaatctttcg taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta
    11521 tcggaatcac agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta
    11581 caaaaggtct caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt
    11641 tggaatcttc cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg
    11701 tcacactggc tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag
    11761 atgcaaaaat ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct
    11821 ggtcttgaaa tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg
    11881 aaaacaagtg aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat
    11941 gatgttttta gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa
    12001 catcccggtg aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt
    12061 gaagaaatcc aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc
    12121 caatgcctca accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt
    12181 caagctcttg cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta
    12241 gacactcagc caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg
    12301 tttccaactt cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt
    12361 gcccgacgaa caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt
    12421 gtacagattg ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat
    12481 cgtagaatca aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc
    12541 ctgaccgcta tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg
    12601 gagccaaaac ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta
    12661 agaaaacgcg gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct
    12721 aaatagactc gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac
    12781 cgttcttcag gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact
    12841 ctcctatagc ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc
    12901 cgtaataagc ctcctagtat ataggcagac tgccaattat aactgattca atccacatta
    12961 tgataaaatc caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac
    13021 gagtcttttc attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa
    13081 agataattag tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt
    13141 tgaatttgtt gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat
    13201 cactgcagct gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta
    13261 ttatcaagca gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct
    13321 gttgattaaa tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt
    13381 actcgaatgt gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc
    13441 taccgaatca ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca
    13501 aatcgttttc tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc
    13561 gcttttatta aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc
    13621 acatgcaatc tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat
    13681 ctttaaatac gtatttgata atttatctta acaaccagat aattttattc agcaccgtat
    13741 tatctatatt gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa
    13801 tcttcttctt gttattaata gtgattagga tgatattcct atattgatct ttgataatta
    13861 gatataattt ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac
    13921 ctagttagcg acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca
    13981 atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
    14041 ctatgtgctt acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta
    14101 taaaatcgaa agcctacttg aataaaaaac aagcctgtga atttctagga attgataata
    14161 atactttaga taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag
    14221 ttcgctttga cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt
    14281 ttcttctcgt cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg
    14341 aggaagtttg cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa
    14401 aaaattcaga gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag
    14461 gcaattagct attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga
    14521 ggagaatgca aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc
    14581 aaaatggctt cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa
    14641 tgtaacaatt agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga
    14701 atatgcaatg aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa
    14761 atcgaaagaa tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat
    14821 tgccgaaata ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac
    14881 cgcgcctaag aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc
    14941 atcagaagag ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct
    15001 tacacttcac gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga
    15061 gacttatgct ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata
    15121 tgctttagat aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca
    15181 ggcaccagaa gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt
    15241 gttgaaacta aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc
    15301 tggagaagta aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa
    15361 aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac
    15421 tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga
    15481 tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga
    15541 agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt
    15601 ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa
    15661 tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg ttttttacgt
    15721 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
    15781 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
    15841 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
    15901 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
    15961 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
    16021 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
    16081 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
    16141 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
    16201 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
    16261 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
    16321 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
    16381 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
    16441 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
    16501 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
    16561 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
    16621 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
    16681 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
    16741 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
    16801 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
    16861 gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
    16921 tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
    16981 gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt
    17041 cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg
    17101 ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga
    17161 ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt
    17221 ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt
    17281 cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta
    17341 actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg
    17401 gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa
    17461 ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt
    17521 ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg
    17581 agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa
    17641 ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac
    17701 aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc
    17761 tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca
    17821 gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta
    17881 acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc
    17941 acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag
    18001 acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc
    18061 gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg
    18121 atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc
    18181 atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc
    18241 tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg
    18301 atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa
    18361 acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa
    18421 ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa
    18481 acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac
    18541 ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa
    18601 ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc
    18661 aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa
    18721 ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg
    18781 cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc
    18841 gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc
    18901 caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga
    18961 aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg
    19021 atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc
    19081 tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa
    19141 tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta
    19201 agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg
    19261 ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat
    19321 tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat
    19381 tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc
    19441 cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt
    19501 ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt
    19561 gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt
    19621 atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat
    19681 cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt
    19741 gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc
    19801 tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga
    19861 aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta
    19921 ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc
    19981 aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca
    20041 atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt
    20101 tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc
    20161 tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc
    20221 ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg
    20281 cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc
    20341 ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc
    20401 taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat
    20461 aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac
    20521 ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat
    20581 ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc
    20641 tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga
    20701 taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat
    20761 aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca
    20821 gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct
    20881 tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc
    20941 tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca
    21001 ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg
    21061 cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt
    21121 gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt
    21181 ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat
    21241 gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc
    21301 tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga
    21361 gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac
    21421 ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat
    21481 tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa
    21541 cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag
    21601 ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta
    21661 gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt
    21721 tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa
    21781 atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt
    21841 gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt
    21901 ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat
    21961 tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt
    22021 tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt
    22081 gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac
    22141 ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt
    22201 ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca
    22261 atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt
    22321 ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt
    22381 ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag
    22441 cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca
    22501 gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa
    22561 gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt
    22621 cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt
    22681 ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag
    22741 agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc
    22801 taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag
    22861 gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat
    22921 ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa
    22981 attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag
    23041 atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat
    23101 agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata
    23161 cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa
    23221 aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc
    23281 cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc
    23341 atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag
    23401 ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc
    23461 ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc
    23521 tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt
    23581 gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg
    23641 ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt
    23701 ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca
    23761 gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg
    23821 atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc
    23881 gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga
    23941 catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt
    24001 cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa
    24061 ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg
    24121 gaatacacct gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt
    24181 ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg
    24241 ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc
    24301 ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc
    24361 aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg
    24421 tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc
    24481 aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac
    24541 taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct
    24601 tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat
    24661 ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt
    24721 cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg
    24781 atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc
    24841 acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa
    24901 accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga
    24961 tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc
    25021 agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg
    25081 aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg
    25141 aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca
    25201 agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac
    25261 gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc
    25321 ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat
    25381 ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca
    25441 gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat
    25501 acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca
    25561 ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct
    25621 tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt
    25681 tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc
    25741 ttcatttatt tctctctcct ttgcttattt acttattgtt attctttctc tattctttct
    25801 ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct
    25861 gataaatttt cacttcattt tctcctcgca aacgacattc atataccata taaccttcat
    25921 actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt
    25981 ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg
    26041 cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat
    26101 gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt
    26161 cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat
    26221 ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa
    26281 ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc
    26341 ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc
    26401 gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga
    26461 tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt
    26521 cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc
    26581 ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac
    26641 gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct
    26701 ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa
    26761 taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg
    26821 ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag
    26881 attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc
    26941 gatccctatt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc
    27001 tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc
    27061 tagcgtgtaa tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat
    27121 cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc
    27181 gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc
    27241 taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag
    27301 tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata
    27361 agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt
    27421 accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc
    27481 ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa
    27541 tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg
    27601 tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc
    27661 taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc
    27721 cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg
    27781 gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga
    27841 ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag
    27901 caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag
    27961 aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca
    28021 ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg
    28081 cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac
    28141 ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa
    28201 cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt
    28261 atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac
    28321 tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt
    28381 caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct
    28441 tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa
    28501 tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt
    28561 tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc
    28621 ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac
    28681 tttattcgtc agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac
    28741 aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca
    28801 agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat
    28861 agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat
    28921 aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct
    28981 atctactaac agatcattga cgattccata actattattg aacatatatt tccaagcaaa
    29041 aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct
    29101 aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc
    29161 tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact
    29221 taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg
    29281 catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa
    29341 taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg
    29401 acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac
    29461 cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac
    29521 atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat
    29581 atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa
    29641 agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta
    29701 accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac
    29761 ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt
    29821 ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg
    29881 atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga
    29941 aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt
    30001 gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga
    30061 caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt
    30121 tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt
    30181 aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa
    30241 aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata
    30301 ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga
    30361 acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt
    30421 ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact
    30481 gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa
    30541 cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc
    30601 agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg
    30661 aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa
    30721 tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt
    30781 ttattatttt cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc
    30841 aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg
    30901 ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa
    30961 aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt
    31021 tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg
    31081 gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc
    31141 gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa
    31201 tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt
    31261 tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta
    31321 tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt
    31381 tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat
    31441 tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga
    31501 aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa
    31561 gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa
    31621 aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag
    31681 ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta
    31741 tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag
    31801 cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt
    31861 taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt
    31921 ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat
    31981 ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt
    32041 cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg
    32101 tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat
    32161 aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa
    32221 ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt
    32281 gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat
    32341 gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag
    32401 aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca
    32461 tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg
    32521 cttactgtca atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa
    32581 gcttatgtat caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag
    32641 ctattcctaa attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat
    32701 tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg
    32761 cgaaaaaagc taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata
    32821 tctctgataa agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga
    32881 gcaaaattgc aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc
    32941 gtacttctca tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg
    33001 ccgtttctga acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag
    33061 agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa
    33121 gctctacgat ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg
    33181 taatcggaaa agtcggaaac gactacacga acatctactc agtggacacg gataatttcc
    33241 aggatagtta cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc
    33301 tccatgctcc gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct
    33361 tatttgatca ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag
    33421 aagatagcat ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt
    33481 tttctaccga tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc
    33541 agatacctga tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt
    33601 cgccacagtt gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac
    33661 ttttattcca actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg
    33721 agattactct cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac
    33781 agaaacactg aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac
    33841 cccctcccgg tattttagcg aatttttctt attttccatc tgttttagat agctgtggat
    33901 aactataatg tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca
    33961 cgttgtgtat aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca
    34021 tgttatttgg ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt
    34081 ttgttttatt cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta
    34141 tcttcccatc aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac
    34201 ttctcttgga agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat
    34261 cctttcccac ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt
    34321 gacaataatt tgtcacaacc tcttttt
//
LOCUS       DACEDD010000029        33842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEDD010000029 DACEDD010000000
VERSION     DACEDD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.029"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            324..1985
                     /locus_tag="IWO45_001529"
     CDS             324..1985
                     /locus_tag="IWO45_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0333007.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2055..2402)
                     /locus_tag="IWO45_001530"
     CDS             complement(2055..2402)
                     /locus_tag="IWO45_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0333008.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2486..3427
                     /locus_tag="IWO45_001531"
     CDS             2486..3427
                     /locus_tag="IWO45_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0333009.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3428..4471
                     /locus_tag="IWO45_001532"
     CDS             3428..4471
                     /locus_tag="IWO45_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0333010.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4477..5547
                     /locus_tag="IWO45_001533"
     CDS             4477..5547
                     /locus_tag="IWO45_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333011.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5537..6490
                     /locus_tag="IWO45_001534"
     CDS             5537..6490
                     /locus_tag="IWO45_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0333012.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6502..7512
                     /locus_tag="IWO45_001535"
     CDS             6502..7512
                     /locus_tag="IWO45_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0333013.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7797..8618)
                     /locus_tag="IWO45_001536"
     CDS             complement(7797..8618)
                     /locus_tag="IWO45_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0333014.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8715..8993)
                     /locus_tag="IWO45_001537"
     CDS             complement(8715..8993)
                     /locus_tag="IWO45_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333015.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9261..11270
                     /gene="metG"
                     /locus_tag="IWO45_001538"
     CDS             9261..11270
                     /gene="metG"
                     /locus_tag="IWO45_001538"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0333016.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11749..12336)
                     /locus_tag="IWO45_001539"
     CDS             complement(11749..12336)
                     /locus_tag="IWO45_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333017.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12511..13833
                     /locus_tag="IWO45_001540"
     CDS             12511..13833
                     /locus_tag="IWO45_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0333018.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13988..14764
                     /locus_tag="IWO45_001541"
     CDS             13988..14764
                     /locus_tag="IWO45_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0333019.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14757..15326
                     /gene="rnmV"
                     /locus_tag="IWO45_001542"
     CDS             14757..15326
                     /gene="rnmV"
                     /locus_tag="IWO45_001542"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0333020.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15319..16203
                     /gene="rsmA"
                     /locus_tag="IWO45_001543"
     CDS             15319..16203
                     /gene="rsmA"
                     /locus_tag="IWO45_001543"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0333021.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16654..17283
                     /locus_tag="IWO45_001544"
     CDS             16654..17283
                     /locus_tag="IWO45_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333022.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFGLIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17407..18516)
                     /gene="ugpC"
                     /locus_tag="IWO45_001545"
     CDS             complement(17407..18516)
                     /gene="ugpC"
                     /locus_tag="IWO45_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0333023.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18725..19147
                     /gene="mgsA"
                     /locus_tag="IWO45_001546"
     CDS             18725..19147
                     /gene="mgsA"
                     /locus_tag="IWO45_001546"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0333024.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19470..20006
                     /locus_tag="IWO45_001547"
     CDS             19470..20006
                     /locus_tag="IWO45_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0333025.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20046..21809)
                     /locus_tag="IWO45_001548"
     CDS             complement(20046..21809)
                     /locus_tag="IWO45_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333026.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21812..23527)
                     /locus_tag="IWO45_001549"
     CDS             complement(21812..23527)
                     /locus_tag="IWO45_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333027.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24306..24530)
                     /locus_tag="IWO45_001550"
     CDS             complement(24306..24530)
                     /locus_tag="IWO45_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333028.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24730..27498
                     /locus_tag="IWO45_001551"
     CDS             24730..27498
                     /locus_tag="IWO45_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0333029.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27579..27998
                     /locus_tag="IWO45_001552"
     CDS             27579..27998
                     /locus_tag="IWO45_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0333030.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28010..28480
                     /locus_tag="IWO45_001553"
     CDS             28010..28480
                     /locus_tag="IWO45_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0333031.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28497..29276
                     /locus_tag="IWO45_001554"
     CDS             28497..29276
                     /locus_tag="IWO45_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0333032.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29263..30084
                     /locus_tag="IWO45_001555"
     CDS             29263..30084
                     /locus_tag="IWO45_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0333033.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30101..31150
                     /locus_tag="IWO45_001556"
     CDS             30101..31150
                     /locus_tag="IWO45_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0333034.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31163..32167
                     /locus_tag="IWO45_001557"
     CDS             31163..32167
                     /locus_tag="IWO45_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0333035.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32800..33819
                     /locus_tag="IWO45_001558"
     CDS             32800..33819
                     /locus_tag="IWO45_001558"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0333036.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11049 a         5996 c         6913 g         9884 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
       61 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
      121 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
      181 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
      241 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
      301 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
      361 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
      421 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
      481 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
      541 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
      601 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
      661 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
      721 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
      781 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
      841 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
      901 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
      961 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
     1021 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
     1081 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
     1141 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
     1201 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
     1261 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
     1321 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
     1381 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
     1441 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
     1501 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
     1561 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
     1621 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
     1681 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
     1741 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
     1801 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
     1861 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
     1921 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
     1981 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
     2041 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
     2101 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
     2161 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
     2221 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
     2281 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
     2341 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
     2401 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
     2461 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
     2521 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
     2581 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
     2641 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
     2701 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
     2761 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
     2821 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
     2881 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
     2941 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
     3001 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
     3061 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
     3121 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
     3181 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
     3241 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
     3301 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
     3361 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
     3421 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
     3481 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
     3541 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
     3601 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
     3661 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
     3721 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
     3781 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
     3841 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
     3901 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
     3961 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
     4021 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
     4081 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
     4141 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
     4201 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
     4261 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
     4321 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
     4381 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
     4441 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
     4501 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
     4561 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
     4621 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
     4681 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
     4741 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
     4801 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
     4861 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
     4921 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
     4981 ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact
     5041 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
     5101 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
     5161 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
     5221 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
     5281 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
     5341 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
     5401 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
     5461 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
     5521 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
     5581 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
     5641 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
     5701 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
     5761 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
     5821 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
     5881 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
     5941 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
     6001 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
     6061 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
     6121 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
     6181 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
     6241 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
     6301 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
     6361 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
     6421 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
     6481 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
     6541 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
     6601 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
     6661 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
     6721 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
     6781 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
     6841 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
     6901 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
     6961 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
     7021 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
     7081 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
     7141 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
     7201 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
     7261 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
     7321 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
     7381 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
     7441 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
     7501 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
     7561 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
     7621 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
     7681 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
     7741 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
     7801 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
     7861 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
     7921 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
     7981 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
     8041 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
     8101 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
     8161 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
     8221 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
     8281 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
     8341 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
     8401 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
     8461 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
     8521 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
     8581 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
     8641 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
     8701 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
     8761 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
     8821 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
     8881 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
     8941 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
     9001 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
     9061 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
     9121 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
     9181 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
     9241 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
     9301 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
     9361 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
     9421 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
     9481 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
     9541 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
     9601 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
     9661 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
     9721 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
     9781 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
     9841 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
     9901 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
     9961 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    10021 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    10081 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    10141 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    10201 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    10261 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    10321 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    10381 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    10441 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    10501 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    10561 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    10621 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    10681 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    10741 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    10801 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    10861 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    10921 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    10981 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    11041 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    11101 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    11161 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    11221 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    11281 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    11341 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    11401 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    11461 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    11521 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    11581 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    11641 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    11701 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    11761 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    11821 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    11881 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    11941 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    12001 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    12061 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    12121 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    12181 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    12241 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    12301 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    12361 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    12421 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    12481 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    12541 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    12601 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    12661 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    12721 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    12781 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    12841 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    12901 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    12961 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    13021 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    13081 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    13141 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    13201 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    13261 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    13321 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    13381 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    13441 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    13501 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    13561 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    13621 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    13681 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    13741 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    13801 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    13861 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    13921 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    13981 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    14041 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    14101 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    14161 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    14221 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    14281 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    14341 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    14401 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    14461 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    14521 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    14581 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    14641 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    14701 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    14761 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    14821 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    14881 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    14941 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    15001 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    15061 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    15121 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    15181 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    15241 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    15301 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    15361 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    15421 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    15481 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    15541 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    15601 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    15661 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    15721 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    15781 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    15841 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    15901 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    15961 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    16021 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    16081 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    16141 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    16201 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    16261 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca
    16321 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    16381 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    16441 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    16501 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    16561 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    16621 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    16681 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    16741 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    16801 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    16861 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgggtt gattggggct
    16921 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    16981 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    17041 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    17101 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    17161 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    17221 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    17281 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    17341 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    17401 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    17461 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    17521 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    17581 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    17641 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    17701 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    17761 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    17821 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    17881 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    17941 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    18001 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    18061 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    18121 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    18181 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    18241 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    18301 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    18361 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    18421 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    18481 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    18541 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    18601 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    18661 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    18721 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    18781 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    18841 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    18901 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    18961 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    19021 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    19081 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    19141 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    19201 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    19261 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    19321 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    19381 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    19441 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    19501 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    19561 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    19621 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    19681 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    19741 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    19801 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    19861 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    19921 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    19981 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    20041 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    20101 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    20161 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    20221 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    20281 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    20341 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    20401 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    20461 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    20521 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    20581 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    20641 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    20701 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    20761 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    20821 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    20881 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    20941 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    21001 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    21061 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    21121 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    21181 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    21241 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    21301 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    21361 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    21421 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    21481 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    21541 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    21601 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    21661 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    21721 atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    21781 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    21841 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    21901 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    21961 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    22021 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    22081 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    22141 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    22201 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    22261 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    22321 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    22381 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    22441 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    22501 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    22561 tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    22621 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    22681 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    22741 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    22801 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    22861 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    22921 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    22981 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    23041 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    23101 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    23161 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    23221 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    23281 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    23341 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    23401 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    23461 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    23521 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    23581 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    23641 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    23701 aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa
    23761 ttctgcataa aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt
    23821 taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa
    23881 atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt
    23941 gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta
    24001 tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt
    24061 gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt
    24121 aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag
    24181 tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt
    24241 tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt
    24301 gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa
    24361 aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata
    24421 gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga
    24481 aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca
    24541 tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg
    24601 ttttttagaa taaaaattat aagggataag taggctgctg tctataataa gatatatttt
    24661 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
    24721 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
    24781 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
    24841 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
    24901 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
    24961 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
    25021 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
    25081 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
    25141 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
    25201 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
    25261 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
    25321 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
    25381 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
    25441 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
    25501 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
    25561 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
    25621 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
    25681 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
    25741 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
    25801 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
    25861 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
    25921 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
    25981 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
    26041 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
    26101 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
    26161 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
    26221 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
    26281 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
    26341 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
    26401 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
    26461 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
    26521 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
    26581 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
    26641 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
    26701 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
    26761 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
    26821 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
    26881 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
    26941 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
    27001 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
    27061 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
    27121 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
    27181 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
    27241 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
    27301 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
    27361 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
    27421 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
    27481 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
    27541 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
    27601 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
    27661 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
    27721 taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg
    27781 gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg
    27841 ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga
    27901 caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg
    27961 agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt
    28021 gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat
    28081 aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac
    28141 aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc
    28201 gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc
    28261 gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg
    28321 agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga
    28381 agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt
    28441 accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga
    28501 ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag
    28561 ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg
    28621 atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct
    28681 cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg
    28741 ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt
    28801 tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag
    28861 cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg
    28921 gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca
    28981 atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag
    29041 ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac
    29101 aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga
    29161 ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc
    29221 gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc
    29281 aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg
    29341 gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg
    29401 attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta
    29461 gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg
    29521 gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca
    29581 tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg
    29641 attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg
    29701 ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga
    29761 tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg
    29821 acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc
    29881 accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt
    29941 tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta
    30001 ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt
    30061 ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta
    30121 tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag
    30181 agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc
    30241 ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga
    30301 acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt
    30361 gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg
    30421 gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca
    30481 aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc
    30541 gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag
    30601 caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc
    30661 gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca
    30721 gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa
    30781 attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg
    30841 cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa
    30901 taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac
    30961 cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga
    31021 atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt
    31081 agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag
    31141 taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat
    31201 aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga
    31261 aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc
    31321 tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata
    31381 ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac
    31441 cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact
    31501 gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac
    31561 ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta
    31621 tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc
    31681 aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa
    31741 acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta
    31801 ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc
    31861 tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt
    31921 tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat
    31981 ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa
    32041 atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc
    32101 tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga
    32161 ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc
    32221 cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc
    32281 tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg
    32341 caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc
    32401 gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa
    32461 ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat gtccaatctg
    32521 tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa
    32581 tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg
    32641 taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa
    32701 agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact
    32761 ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt
    32821 cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat
    32881 gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat
    32941 cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca
    33001 gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca
    33061 gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa
    33121 ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa
    33181 gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta
    33241 catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa
    33301 aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa
    33361 actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga
    33421 ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt
    33481 ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg
    33541 atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag
    33601 ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg
    33661 acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca
    33721 atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta
    33781 cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc
    33841 gt
//
LOCUS       DACEDD010000030        33588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEDD010000030 DACEDD010000000
VERSION     DACEDD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.030"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..113)
                     /locus_tag="IWO45_001559"
     CDS             complement(<1..113)
                     /locus_tag="IWO45_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0333037.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSS"
     gene            complement(110..499)
                     /locus_tag="IWO45_001560"
     CDS             complement(110..499)
                     /locus_tag="IWO45_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0333038.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(599..739)
                     /locus_tag="IWO45_001561"
     CDS             complement(599..739)
                     /locus_tag="IWO45_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0333039.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(732..1079)
                     /locus_tag="IWO45_001562"
     CDS             complement(732..1079)
                     /locus_tag="IWO45_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333040.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            complement(1093..1587)
                     /locus_tag="IWO45_001563"
     CDS             complement(1093..1587)
                     /locus_tag="IWO45_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333041.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1587..2036)
                     /locus_tag="IWO45_001564"
     CDS             complement(1587..2036)
                     /locus_tag="IWO45_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333042.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(2040..2660)
                     /locus_tag="IWO45_001565"
     CDS             complement(2040..2660)
                     /locus_tag="IWO45_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333043.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(2660..3568)
                     /locus_tag="IWO45_001566"
     CDS             complement(2660..3568)
                     /locus_tag="IWO45_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0333044.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3581..6355)
                     /locus_tag="IWO45_001567"
     CDS             complement(3581..6355)
                     /locus_tag="IWO45_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0333045.1"
                     /translation="MDKDVYFFDDSQKLIKIVGEDKLFSVVQEKEITPSKDELINDKL
                     AVSMEFDDEIKESAYMAVRESESSFSMYKIIGIADPGSLLIFTGINFGPDELDAYIIN
                     DIRPANEFFQKTIQRVIDFTLGEWRVGHLDSTLPAVSMTFYYCSIREALKNLQTLGCE
                     IVFRCNLSGEGITDKWIEVYKQIGEYSNERYEYGDKALTIEKEVDRSNIYTSLIGRGR
                     GEEVGDGYGRRIEFDQVYWSKSKGDPLNKPTGQIYLEIPEMTEKYGIPTKNGKRRKRE
                     KVIIFEDCEDPVELIQLTYQELVNCSRPLVQFKATIFGADSLGNIIRIHRDDRGYHYE
                     TRIFSVKIDRLTGKVETGLGDNLNTSSTRQASNTQTAIQTLDEKKMTFYESTEVSKWQ
                     SDIIRGAKGGSIIMMNPWDTGKGESRQPYQMVWMNGDSIDTSNHFLVANSEGIGFIDG
                     KFNESNFKTAWTIDGNFNANYIQSGRIRADIFETSFNAVGDQLKLVKGALQIVNSNKK
                     IMELTKKGMEFWNTKESIGTIGTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILI
                     SPKVGKGLVLLGNGKAIYFGDLDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEK
                     RAWKIWTMLKARGYSEYAAAGILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPL
                     VGSPTWNGREYVQRLMNTAGIQEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKS
                     INDAKSAAYAFEMNFERPASAHPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTV
                     TSECGWRTSPINGGQEFHNGIDLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIK
                     HNNGKWTGYAHLSRIDVSVGQKVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVD
                     FENPRNYIQF"
     gene            complement(6355..7137)
                     /locus_tag="IWO45_001568"
     CDS             complement(6355..7137)
                     /locus_tag="IWO45_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0333046.1"
                     /translation="MEWHNPMYEFRDTVKNDSQKTWIPTSALNYDGKFIENYIEGYQT
                     LYVEGREMVSLEIESEAVSIGVRISSQRLPERILTIHFKLEEKNPIEFQRSFNKLMRL
                     LYRDKDVEIHFNDELDMYYYGRYQTCDNIPGNVHSVISSFSIICSDPRKYTRIFETNG
                     IVSEYLPYEVAPISISLKANNDGSLRITNGRQNISVTNSMIKKGDFIEMDIAEGKVFV
                     NGVNKTRILDLTSSFKNFMVRTGDLVKCDNGTPLIRYRGVWL"
     gene            complement(7155..9977)
                     /locus_tag="IWO45_001569"
     CDS             complement(7155..9977)
                     /locus_tag="IWO45_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0333047.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEEKSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEEQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQRLVVA
                     IGAIAIAIGPVLYALGMLVKAFQTMKVGLGVLGNGISLFKKLGSAIGFLTSPVGLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKAADWIKNKWTETKEFFSSIWDGIKEAASSAWEGIVNILTP
                     YVIAIKNVFQPMIDFFTNLWSQIGSIAGSAWEIIKTAVMGPILLLIDLITGNFNQLKE
                     DASMLWTTLTTNIQNIITTFVDIVVGYYTALKDTVINIWNVLTSTIKDVWNSFTTWIK
                     ETTNNIVNSIKQGWNNLKQGTIDLFNNMIQGAKDLWNSFKAWFINLVIGTKDNIIQGW
                     ENLKQGTIDTFNNLVNGAQEVWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIRYDRKLLIPAGQAIMNGLNAGLTNGFAS
                     VQSNVGNMANMIADSFTRTPDIDLSANLKNANRNFTTQIEHSVNYGKNKRPAVFNIRL
                     GNQVFEAFVEDISNIQGKEADINLLF"
     gene            complement(9994..10281)
                     /locus_tag="IWO45_001570"
     CDS             complement(9994..10281)
                     /locus_tag="IWO45_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333048.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(10335..10703)
                     /locus_tag="IWO45_001571"
     CDS             complement(10335..10703)
                     /locus_tag="IWO45_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333049.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNANTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            complement(10749..11261)
                     /locus_tag="IWO45_001572"
     CDS             complement(10749..11261)
                     /locus_tag="IWO45_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAQ0333050.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDD
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(11272..11661)
                     /locus_tag="IWO45_001573"
     CDS             complement(11272..11661)
                     /locus_tag="IWO45_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAQ0333051.1"
                     /translation="MIKTRDQSLFDEMFARSLALGYQTYDYKPLDDVGYPFVELESSQ
                     TVHNPNKTDIKGTVNLTISVWGLQKKRKQVSDMASALFNQALQIQATEGYYWDLNYQA
                     SGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(11658..12029)
                     /locus_tag="IWO45_001574"
     CDS             complement(11658..12029)
                     /locus_tag="IWO45_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0333052.1"
                     /translation="MKVSLGYKGVDELLKHLEEAVTLRDVQMVVKTNGAELTKRMQEK
                     ARFNGHWEGDVFVHPTGFTRRSIRMWLLEGGFVAQVGPQSDYSPYLEYGTRFMSAQPF
                     VGPAFNVQKAIFMKDMQRLFK"
     gene            complement(12026..12340)
                     /locus_tag="IWO45_001575"
     CDS             complement(12026..12340)
                     /locus_tag="IWO45_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333053.1"
                     /translation="MRFLDEVIFEKDGEGNRYDPELGEWIEEAPITVTTNANVTDLGT
                     DRSVAIFGDIRQGAKVIRTMPLFVVPKYDRIIFNGKTYKDVAQRSLLSTNSIIVQEVI
                     AK"
     gene            complement(12337..12666)
                     /locus_tag="IWO45_001576"
     CDS             complement(12337..12666)
                     /locus_tag="IWO45_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAQ0333054.1"
                     /translation="MVIKDDVKKLLSGSTDDKLEVIERRTRERLASLLGVSVTPESLE
                     YIVFDVTNKRFNRIGQEGMTSYAQEGLSMAFPDSDFSEYASEIDDFKRKDDEELYKPR
                     KGSVRFL"
     gene            12716..13003
                     /locus_tag="IWO45_001577"
     CDS             12716..13003
                     /locus_tag="IWO45_001577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333055.1"
                     /translation="MCDPSSVFIVVVYLPAGTVSVQVILPLEETVNPLVTGAIEYVTV
                     LFVAFCGATVAVNCLLPAVPATALEVLGVTVTPITGIVTPPPVQFYRTQHL"
     gene            complement(12960..13847)
                     /locus_tag="IWO45_001578"
     CDS             complement(12960..13847)
                     /locus_tag="IWO45_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0333056.1"
                     /translation="MAQTWNPDNVTVYETKEGKIPDKYNTLIINDIMENSKVMQLAKY
                     EEMTDKEKKFEYFAEGPGAYWVGEGEKIQTSKPKWLQATMVAKKLGVIIPVSREYLHY
                     KMSDFFTVMQPKIAEAFYKKFDAAALLNTDNPFPQSLEESVVAAANVIKGPLTYDNIL
                     ALEDALGKNEFEPNAFISNRKNRTELRSAAQTVGTNVEFIYDRSANTIDGLPVVDLKA
                     LGKGELYAGDFNYMFYGIPFNISFKISEDAQLSTLKNEDGTPVNLFEQELVALRATMD
                     VGFMIVKDAAFGKIEPAGE"
     gene            complement(13862..14464)
                     /locus_tag="IWO45_001579"
     CDS             complement(13862..14464)
                     /locus_tag="IWO45_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0333057.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPTEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELEAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(14767..14994)
                     /locus_tag="IWO45_001580"
     CDS             complement(14767..14994)
                     /locus_tag="IWO45_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333058.1"
                     /translation="MHLYQNDAQFKEDYDAVKQHLIDVLEPIVKEVYETARKVIEPVV
                     QSITELVQSNPEYFKALQNNMNQSVFQSASM"
     gene            complement(15072..16028)
                     /locus_tag="IWO45_001581"
     CDS             complement(15072..16028)
                     /locus_tag="IWO45_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0333059.1"
                     /translation="MKSQDYFIKREKAWQEQQIKDDKKRMNEIKKRLQYAQDAIQKEI
                     DAQWNSFSNGQKITRSEAMKRANEMDVKAFARKAKKYVKEKDFSPTANKELKLYNLTM
                     RVNRLELLKANIGLELIATFNELDKYFSNELTKAGLDELKRQAGILEMAIAKSGYAKL
                     VEQVINSSFRADGFATFSERLWMYQAELKADLDKLLVRSVTMGRNPKQLAPELSKFLT
                     EEGRENTKFNTQRLMVTETTRVQSGIQRESYLDANIEKYGWVTEPNACRLCKSIAADG
                     PYLVSEMEVGKNMMPVHPFDRCSTYPIVDRDAFEKSLKERRL"
     gene            complement(16033..16296)
                     /locus_tag="IWO45_001582"
     CDS             complement(16033..16296)
                     /locus_tag="IWO45_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333060.1"
                     /translation="MKARKKPVTVEIVQLKMLSARSYRKCKEFVGEAWIDHNNMPNGL
                     PGIETLEGTMEISDGDYIIKGVHGEFYPCKPDIFIETYEIIEE"
     gene            complement(16283..17779)
                     /locus_tag="IWO45_001583"
     CDS             complement(16283..17779)
                     /locus_tag="IWO45_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0333061.1"
                     /translation="MDKVNEFEYGTDIHYSNDVNTNYVKFSVESNLHYRFSSAEDLLN
                     DSNTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKDRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNAETSADRGVFDSDKETDTEVEEDEST"
     gene            complement(17792..19036)
                     /locus_tag="IWO45_001584"
     CDS             complement(17792..19036)
                     /locus_tag="IWO45_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAQ0333062.1"
                     /translation="MGLVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDIAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRVKRMF"
     gene            complement(19023..19556)
                     /locus_tag="IWO45_001585"
     CDS             complement(19023..19556)
                     /locus_tag="IWO45_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAQ0333063.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(19583..19807)
                     /locus_tag="IWO45_001586"
     CDS             complement(19583..19807)
                     /locus_tag="IWO45_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333064.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(19814..20026)
                     /locus_tag="IWO45_001587"
     CDS             complement(19814..20026)
                     /locus_tag="IWO45_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333065.1"
                     /translation="MNENQLRELFKTNEANQTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSANNQQRE"
     gene            20509..20688
                     /locus_tag="IWO45_001588"
     CDS             20509..20688
                     /locus_tag="IWO45_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ0333066.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            complement(20681..21094)
                     /locus_tag="IWO45_001589"
     CDS             complement(20681..21094)
                     /locus_tag="IWO45_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333067.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(21355..21771)
                     /locus_tag="IWO45_001590"
     CDS             complement(21355..21771)
                     /locus_tag="IWO45_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0333068.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHVNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            complement(21847..22143)
                     /locus_tag="IWO45_001591"
     CDS             complement(21847..22143)
                     /locus_tag="IWO45_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333069.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDIKLINDK"
     gene            complement(22167..22472)
                     /locus_tag="IWO45_001592"
     CDS             complement(22167..22472)
                     /locus_tag="IWO45_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333070.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(22488..22685)
                     /locus_tag="IWO45_001593"
     CDS             complement(22488..22685)
                     /locus_tag="IWO45_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333071.1"
                     /translation="MNKRQKNKQFKKQLIKNGYPDKKVKLYCSECGNRLSFKNEYHKR
                     HLLCNEFCFMHSVGLGWNDFL"
     gene            complement(22682..22981)
                     /locus_tag="IWO45_001594"
     CDS             complement(22682..22981)
                     /locus_tag="IWO45_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333072.1"
                     /translation="MSDYLTAEQYEYARKTGDWESGKVLTHGQALNDNQKIVLEWLEE
                     YYAKSQWPFPFYVLKHAANDMPTNIFGFYDDFTMKEEFEVLAAFAQWGLKQEEAE"
     gene            complement(22978..23562)
                     /locus_tag="IWO45_001595"
     CDS             complement(22978..23562)
                     /locus_tag="IWO45_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0333073.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDNLTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDISRSLC
                     TWEKSAYQLTEAEIKEIDERYWPFAVPVEEVVER"
     gene            complement(23559..23972)
                     /locus_tag="IWO45_001596"
     CDS             complement(23559..23972)
                     /locus_tag="IWO45_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333074.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGMKDKNGVDIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            complement(23969..24214)
                     /locus_tag="IWO45_001597"
     CDS             complement(23969..24214)
                     /locus_tag="IWO45_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333075.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(24174..24530)
                     /locus_tag="IWO45_001598"
     CDS             complement(24174..24530)
                     /locus_tag="IWO45_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0333076.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(24530..24835)
                     /locus_tag="IWO45_001599"
     CDS             complement(24530..24835)
                     /locus_tag="IWO45_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333077.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(24832..24993)
                     /locus_tag="IWO45_001600"
     CDS             complement(24832..24993)
                     /locus_tag="IWO45_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0333078.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(25008..25367)
                     /locus_tag="IWO45_001601"
     CDS             complement(25008..25367)
                     /locus_tag="IWO45_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0333079.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(25364..26215)
                     /locus_tag="IWO45_001602"
     CDS             complement(25364..26215)
                     /locus_tag="IWO45_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0333080.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(26232..27062)
                     /locus_tag="IWO45_001603"
     CDS             complement(26232..27062)
                     /locus_tag="IWO45_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0333081.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(27065..27751)
                     /locus_tag="IWO45_001604"
     CDS             complement(27065..27751)
                     /locus_tag="IWO45_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333082.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(27757..28428)
                     /locus_tag="IWO45_001605"
     CDS             complement(27757..28428)
                     /locus_tag="IWO45_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0333083.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGDFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(28421..28762)
                     /locus_tag="IWO45_001606"
     CDS             complement(28421..28762)
                     /locus_tag="IWO45_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333084.1"
                     /translation="MENELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(29088..29276)
                     /locus_tag="IWO45_001607"
     CDS             complement(29088..29276)
                     /locus_tag="IWO45_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333085.1"
                     /translation="MSYTLQQEHQILRLIKQRRKQLQDDREALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(29297..29581)
                     /locus_tag="IWO45_001608"
     CDS             complement(29297..29581)
                     /locus_tag="IWO45_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333086.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVRKVLGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(29559..29771)
                     /locus_tag="IWO45_001609"
     CDS             complement(29559..29771)
                     /locus_tag="IWO45_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333087.1"
                     /translation="MSQDFILKVRVALATHNKSQAWLAEKLDISTAYMSDIMNGRRKP
                     DKQIKPIEAVLAELEKEKKHANNNSR"
     gene            complement(29774..30550)
                     /locus_tag="IWO45_001610"
     CDS             complement(29774..30550)
                     /locus_tag="IWO45_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0333088.1"
                     /translation="MNTPQIFNFGQQEVRTVLLNDEPYFVGKDVAEILGYERADNAVR
                     NHVDEEDRLMHRISASGQNRNMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEER"
     gene            complement(30676..30897)
                     /locus_tag="IWO45_001611"
     CDS             complement(30676..30897)
                     /locus_tag="IWO45_001611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333089.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPREHQKNSFATG
                     EKKTQKRSEKVLRIIQFLLEKIRHFGNLF"
     gene            31195..31542
                     /locus_tag="IWO45_001612"
     CDS             31195..31542
                     /locus_tag="IWO45_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333090.1"
                     /translation="MTTFERVKMLADKRKISIVELEEKLNFSKNSLYAWKKSKPSIDK
                     LNAVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREIL
                     SAMIEAYLEKKDN"
     gene            31578..31973
                     /locus_tag="IWO45_001613"
     CDS             31578..31973
                     /locus_tag="IWO45_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0333091.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            32027..32338
                     /locus_tag="IWO45_001614"
     CDS             32027..32338
                     /locus_tag="IWO45_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333092.1"
                     /translation="MIYTEFKEWLEKNTTGYETFIVKATNYQIEKNKNRPPKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            32350..33498
                     /locus_tag="IWO45_001615"
     CDS             32350..33498
                     /locus_tag="IWO45_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0333093.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
BASE COUNT         9440 a         7161 c         5215 g        11772 t
ORIGIN      
        1 gaactacctt cgtttgcgcc caaattgaat tcattgttga tcgagtaagc aaaggcgttt
       61 attggcaata aaaaaagagc tataatagct cctacaaaga ttttcttttt catttggttc
      121 ctcctcggtc tttattttca gtatcatttc gtatttgttg gaatacctct tttaacttat
      181 ctggcaaagg aaggaatacc gaaatgtttt ctaaaaaact aatcccttca tttgcaacat
      241 agaacataat aactatttct cttagaggta tagaattccc aacgattttc tgtacgtcaa
      301 ccgcaacggc aatcacaacg aagattaaga ctttcttcaa caatccttcg aaacctatct
      361 cactacttat ttctttcaat ttccatgcct tagcaagtcc agttaagaag tccacaccca
      421 caagaaaaat taaaacatgt agtatattgt ccatccctcc caataaacca acaattgtcc
      481 cgcctatcac ccctgtcatt actgagagtg tatttaaata tttttccaat atctatttct
      541 cctttatgca aagaagcgca ctcgtttgag tacgcttttt atttaacttt gtgcttaatt
      601 aaatttcatt tccagtaatc tgcttatatt ggtcttttgt taaacagttc atgtttacat
      661 agtcaagtaa atcttggtcc gtataacatc cccaatcata aaattgtttt atattttcaa
      721 agcccggaaa actattcatt acttttccct ccattcttaa tacttgcgac ttccatcgtc
      781 aattgagcta acatttgttg cattttttct tgttcctgta ccgatatcgc caattgtttt
      841 gtcagtagag catttaattg cttcaactga tcgacatctt caatatcgtc aaccgctgtg
      901 ccactcttct cttcttcagt agctaactca acccactgtt ttttatcaaa atcaaatctt
      961 ggtctccagt taggaactgg tggtttaatc tctgtacagt tttcagggat attttcttga
     1021 ttgttcaaaa tgatttgctc gaatccgtaa ggcttgattg atttgtaaac tactttcatt
     1081 agatttcctc ccttaaattg aataagtgat aacaaatgaa taagctgatc cataacttga
     1141 atttcttctc catttaatgg ctccgtctgc gccaatagat agttgagcac tgttcaaagt
     1201 agaacggtct atcgagccaa ccagttgctc aaaactaatt ggtggccgat agccttctgg
     1261 aattgttagt atcgttgaat catttccacc actgcttttt ccgtttaaag ccacaaaata
     1321 tatagaaact gtttttcctt cacgataaag ctttgctgtc cctgtattac cgttagtgac
     1381 aaccagattt accacttcat atctgttatc atcagtcgtc aaaacacgtt tattattgat
     1441 tgttacagaa cctgaaaaat tcttatgtcc tgcaatggct tggtctccat ctgtctgcac
     1501 caatgcttct ttaccatcaa tagcttttac atgcgttttt aagtatttag caactccgtc
     1561 ttcttttaat tgaacaatat cagccattaa accgtcccca ctttctcaaa tgtaatattg
     1621 gctaatccat caagtttcac tttatcggtt gctgacatta aacctgctgt cgtagttgta
     1681 gcattacctg gatttttctg tgctccagct gcaattccat ccaacttagt tttatctgtg
     1741 gacgacatca acccatttgc tgtagttgta gctacagctg tagttgtggc atttattcca
     1801 ggatcacctt tatctccctt tggtaaaaca aaattaaatc tagctgcaga tgatgttcct
     1861 acattcgtaa cagaagcggt tgaaccacta gaaacggttc ctatggtaat tgttgctgct
     1921 tggccaggat cgcctttatc tcccttcacc gttgttggtt tgccttctat agcattccaa
     1981 tgagtttgtg gataaacctg tacattgctt tgttttattt ttacgatatc tgtcattctc
     2041 tatacctccc caattttttc aaaagtaaaa tttggaattc tttcgtttgt gtaattttct
     2101 gcttgattta cagcttcttg gaatttttga tctacatatg actgattgac accaccagtc
     2161 ccactaccac ctgtagaact aattgttcca tcttcatcaa tagtgatgtt ttctccagca
     2221 gtataatttt tcagtgaatc gagtttttct tttaactcca gcgaaaaatt gacatcagat
     2281 tgtctgactg cagagacttt ccctgtctca tcaatcgtca ataattcacc aacttttatg
     2341 ccacccaaac ggtcgatagt ggcaactggc aacacgtagt caccttctcc actgtttaaa
     2401 attttttgat acatttctgc cgtgataatg ccgtcatctt cttcacttgc atagggtaat
     2461 tctgtgagaa cattctctaa tcccaaatct tctttagtaa tgacgacggc tccaattttt
     2521 ccattaacag ataaaactgt tgcttctcct gctattattt cttctaaccc aagaactgcg
     2581 gaagcatgtg tgattggaaa gaattggcgt tgtacacccg ttttttcatc cgtttccatc
     2641 attcgtttag ttttagccat tagatcaccc caactttctc aagagtgaac acattttgct
     2701 ttggatcatc aacggttgcg atgatcaagg ctccttcttc aatcgggaat gctacagttc
     2761 caactttttc gacttcatga ttatctgaaa agagatcatc ctgcaaaata tcagtcactt
     2821 caatctcccc gtattcaatc gtgaaaagcg ttgctcgcaa tttctgatat aaatagtcca
     2881 tatctgccag caaacgctct gaaatagacg catgacgaac tccttgaata tctactcgcg
     2941 catccgttag ttcggctaac atgactccac ctggatcaat cgtctttaaa atatccttga
     3001 ttgattcaaa ccatgccaaa tagtctgatt cttgtccttc tctccaatct tggaaactgt
     3061 gttcctgttc ttgtctccaa cgttcaaatt cttctttcct ttcattcatc cagtccgtaa
     3121 aatcgccttt attttcatta ataaagtccg tcatatccgc aattaaatct tcgattgatt
     3181 gccaataaga acccatttct ccttctgttt ttgaaacagc attaatgaca aaataagaga
     3241 agtcttgagt tgttccaatc aagtcttctc ctttaaatat aatgaaattg gctgtttgtc
     3301 gatgtaaaca ctgcatggaa tatttatcaa aaatatagtt aatttttcct tttttagcat
     3361 ccacaatttt tgtttctaat tggactggat atttcccacc tacaactgat tcaaaatata
     3421 ccttacattc tgataaatcg tagggaagac cattttcaac tatagtcgct tccataacct
     3481 ctgtattctt attgccttgt cgaacttgaa tcatccccac ataattatat ggttctgttg
     3541 tacttaaaac gacattccat ttactcatag aatcaccacc ttaaaattgg atataatttc
     3601 ttggattctc aaaatcaaca actggctgtg gccaataatt tttcataatt tgaaaatgta
     3661 gatgttctcc tgtagatgga ccagttgttc ccatcaagcc tatttgagca cctttctgga
     3721 ctttttgtcc aacagaaaca tcaatacgag acaaatgagc ataacctgtc cacttcccat
     3781 tattatgttt aataaccacg taattgccat accagtcata ataattagca ccagcctgaa
     3841 caacttcccc atctaatgct gcaaaaacag gtgtattagg atttccattt acaagatcaa
     3901 ttccattatg aaattcttgg ccgccattta ttggactagt tctccatcca cattctgaag
     3961 taactgtcac tggtttttga attggacaaa tgaaattccc tccaggttct ggactagtta
     4021 atccatgtaa tttgttatac caagattggg cataattttg gcgttctgga tgtgcagaag
     4081 ctgggcgttc gaagttcatt tcaaaagcat acgctgcact tttggcatca ttgatcgatt
     4141 taaatcctga tactgtggtt ggatttactt ttccgagcca ttgaccgttg aacatacacc
     4201 aatctaataa ttttacttgg gcttcaatac ttcgataatc ttcttgaatc cctgcggtgt
     4261 tcatcaaacg ctgaacatac tctcgtccgt tccaagttgg tgatccgact aacggataag
     4321 cagaaccatc ccattgaaca atcccataag ctggaccacc aagttgttct gtatcagggt
     4381 tcatacttgc tccaacctcc ccctgaatat ttcctagaat acctgcagct gcatattcag
     4441 aatagccacg agctttcagc attgtccaaa ttttccatgc tcttttttca gcttccgtcg
     4501 tcaattgagg agggatagtt ccaggatcac ttcctccact gttgttccct gtgatttctt
     4561 ttccgttaac tgtgagtttg ccttgtacat ctaagtctcc aaaataaatt gctttaccat
     4621 ttcctaataa aactaatcct ttaccaactt taggagaaat caaaatatat ttgccgtctc
     4681 catttgtacg aataactaaa gaattatctt caataggtgt gggagtagaa gctccaggaa
     4741 aaggattacc agcagaatca gtagtaccaa ttgtgccaat ggattctttg gtattccaga
     4801 actccatccc ttttttggtt aattccatga tttttttatt gctgtttaca atttgcaaag
     4861 ctccttttac taacttgagc tgatcaccaa cagcgttaaa tgaagtttca aaaatatctg
     4921 ctctaatacg tccagattgg atataattgg cattgaagtt tccatcaatc gtccatgccg
     4981 ttttgaaatt tgactcattg aatttcccat caataaaacc aatcccttcc gaattagcta
     5041 caagaaaatg gttagaagta tcaatactat ccccattcat ccaaaccatc tgatatggtt
     5101 gacgactttc gcctttgcca gtatcccaag gattcatcat gataattgat ccaccctttg
     5161 ctccgcgaat gatatccgac tgccatttag aaacttctgt ggactcataa aaggtcatct
     5221 tcttctcatc aagggtctgt atggcagttt gagtatttga agcttgacgt gttgatgaag
     5281 tatttaaatt atcacctaag ccagtttcga cttttcctgt taatcgatca atcttcacac
     5341 tgaaaattct agtctcgtaa tgatagccgc ggtcatcacg atgaatacgt ataatattac
     5401 ctaaactatc tgctccaaaa atagttgctt taaattgaac tagcggacgt gaacagttaa
     5461 ccaattcttg ataggtaagc tgaattagtt caacaggatc ttcgcagtct tcgaatataa
     5521 ttaccttctc acgcttacga cgttttccgt ttttagtagg aataccatat ttttcagtca
     5581 tctcagggat ttccaaatat atctggccag taggcttgtt taacggatcc ccttttgatt
     5641 tggaccagta tacttgatca aactcaattc gacgaccata accatctcca acctcttcac
     5701 cacggccgcg gccgattaga gaagtataga tgttacttcg atctacttct ttttcaattg
     5761 tcaaggcttt atcgccgtac tcatatcgct cattactgta ttcgccaatc tgcttgtaaa
     5821 cttctatcca tttatccgtg attccttctc cacttagatt gcacctaaaa acaatctcgc
     5881 atcctaacgt ttgtagattt tttaaggctt cacggatact acagtagtaa aaagtcatag
     5941 aaaccgctgg cagagttgaa tctaaatgac caactcgcca ctcacctaat gtaaaatcta
     6001 tgactctctg gatggttttc tgaaaaaatt cgttagccgg acgaatatca ttaatgatat
     6061 aagcatccaa ttcatcaggc ccaaaattaa tcccagtgaa tatcaacaat gaacccggat
     6121 cagcaattcc gataatttta tacatagaaa aagacgactc gctttcacga accgccatat
     6181 aagctgactc tttaatttcg tcatcaaact ccatgctaac tgctaactta tcattaatta
     6241 actcatcttt acttggtgtg atttcttttt cttgaactac cgaaaaaagt ttatcttccc
     6301 ctactatttt aataagcttt tggctatcgt cgaaaaaata aacatctttg tccattacag
     6361 ccacactcct ctatatcgta ttaagggagt gccattatca cacttaacta gatcaccagt
     6421 tctaaccata aagtttttaa atgagctggt aagatcaaga attctcgttt tattcactcc
     6481 attcacaaaa acttttcctt cagctatgtc catctcgata aagtcacctt tttttatcat
     6541 tgagttagtc acactaatat tttgacggcc atttgtaatt cttaagctcc catcattatt
     6601 agcctttaaa ctaattgaaa tcggagctac ttcatacgga agatattctg agactatgcc
     6661 gtttgtttca aatatacgtg tatattttct gggatcagaa cagattatag agaaactaga
     6721 tattacacta tgaacgtttc ctggaatatt atcacaggtc tgataccttc cgtaataata
     6781 catatccaac tcatcgttaa agtgaatttc tacatcttta tccctgtaaa gcagcctcat
     6841 caatttatta aagcttcgtt gaaattcaat aggattcttt tcttctaatt taaaatgtat
     6901 agttaaaatc ctctctggaa gtctttgaga actaatacga acacctatgc tcaccgcttc
     6961 actctcaatc tctaaagaaa ccatttctct accttcaaca tacaaagtct ggtacccttc
     7021 tatataattc tcgataaact tcccatcata attcaatgca gacgtcggta tccatgtttt
     7081 ctgactgtcg tttttaaccg tgtctctgaa ttcatacatt ggattatgcc actccatcat
     7141 atttccctca ctttctagaa caataaatta atatccgcct ctttaccttg aatgtttgaa
     7201 atatcctcaa caaacgcttc aaacacttga tttcctaagc gaatattgaa aactgcagga
     7261 cgtttatttt tgccgtagtt aacactatgc tctatttgtg ttgtgaaatt tctatttgca
     7321 ttttttaaat tcgctgaaag atcaatatca ggtgtacgag taaaactatc tgcaatcata
     7381 ttagccatgt ttcctacatt gctttggaca ctggcaaagc cattagttaa acctgcgttt
     7441 agtccattca tgattgcttg cccagctggg attaacaatt ttctatcgta ccgaataggt
     7501 cctttgtgtt ccctaatcca gtcaccaata cctccaacaa aatcttgcac agatttccat
     7561 gcattttgta acccttctag aaaactatcc atgatagctt ttccggctgc tagtaaatcg
     7621 atatttttca agttatcaaa ccagccagtt actctatcaa ccgtatcact aacagcattt
     7681 actaaattat cccatacctc ttgagcacca tttactaaat tgttgaaagt atctatagta
     7741 ccttgtttta ggttttccca tccctgaatg atgttatcct tagttccaat aactagatta
     7801 ataaaccaag ctttgaaaga attccataaa tctttcgctc cttgaatcat attattaaac
     7861 agatcgattg tcccttgttt taggttattc cacccctgtt taatactatt tacaatattg
     7921 ttagttgtct ctttgatcca tgtagtaaaa gaattccaca catctttgat ggtagaagtc
     7981 aacacattcc agatatttat cacagtatcc tttaaggctg tgtaataacc aacaactata
     8041 tctacaaacg tcgtgataat gttttggata tttgtagtta atgtagtcca cagcatcgaa
     8101 gcatcttctt ttaactgatt aaaattgcct gttatcaaat caatcaaaag tagaattgga
     8161 cccattacag cagtttttat aatttcccat gcagaacctg cgattgatcc aatttgagac
     8221 cataggttcg taaagaaatc aatcattggc tgaaaaacat tttttatggc aataacatac
     8281 ggtgttagaa tgtttacaat tccttcccaa gcggaactgg cagcttcttt gatgccatcc
     8341 catatactcg aaaagaactc ttttgtttca gtccatttat tcttgatcca atctgccgct
     8401 ttcccagggg cttcttgaat tgtagtccaa acattgtctg cgccttcttt aatggacttc
     8461 cataaattgt taaaccattc tcctgtagat ttccatgcat tctgaatcca ttctactgcc
     8521 gagcttacag cagacttgat tccctcccat aagccaatcc aaaagtttct aaaatcttca
     8581 ctcgtattcc aaagatagat gaaacctaca acaagtagtg ctaccgcagc tataaccaat
     8641 ccgactggac tggtaagaaa acctatggcg gaacctaatt tcttgaacaa agagattccg
     8701 ttacctaata cacctaaccc cactttcatg gtttgaaacg ctttaaccag cattcctaat
     8761 gcatacaata ctggcccaat tgcaatagcg attgctccta tggccactac taatctttga
     8821 gttgattctg gagcacttac aaatttttct actaagccgg atatggcatc tgctactttt
     8881 ttgatggatg gtgctagaat cttttgaatt acaatagctg ctgactcaaa agctccaaac
     8941 atttgctcga tggaagaatt catattatct tgcatggtcc gagccatatc gtcagctgca
     9001 ccatcagaat ctttcagaga ttttgttaat ttgcccaatg aatcaggtcc tttatcaatc
     9061 aaagccatca tccctgataa tgattcttgc ccatatagtg ttactaaagc attttgttgt
     9121 tgttcaggcg tcaggccttc aaaagctttt ttaagtaatt ctacttgagt ttttaaaggt
     9181 ttcattttac cgtcagcatc ataaaacgaa acacctaaat tatccattgt atcttgcata
     9241 gcctttgttg gccttgctaa cctagacaat gctcctcgca acgttgtacc tgcttgagaa
     9301 cccttaatac ctgcgtcact cataatacca atagctgctg cagtttcttc caaagaaata
     9361 cccattgaat tggctacagg agcaacatac ttcaatgcct ctcccatgtc tccaacttca
     9421 gcattggtat ccgcagcagc acgagcaaat acatcagcga catgtcctgc ttcacttgct
     9481 tctaaaccaa atcctctcaa agcagtagca gtattttcag aagctagagc cacatcccct
     9541 ccagatacag ctgctaagtc taaaagaccc ggcattgctt tcatgatttc ttgtgcgcta
     9601 aatccagcag aagcaagatt ttccatgccg gcagccgatt cttttgcgct aaaagcagtt
     9661 tttgctccta gatcaatcgc ttgctgtttc atctgttcaa atgtgtcgcc tgttgctcct
     9721 gatatagctt ttacacgact catttgttct tcgaagtcgc caccaacttt agcagctgct
     9781 acgcctactc ctataagagg agtggtaata tactttgtca ttgcggcacc agtaccttgc
     9841 atcactttac ctacagcggt tgtcatacta tttgatttct cttcaaaagt cttaacagca
     9901 tcttgcgcat ctttaaaagt ctttacaaat ccactatctg tggcttttaa taaggcttca
     9961 acagaaaatt gttccatgat tttcctcctt tcctcaagag ttagctttag ttagtaagct
    10021 ttggaatttt ttatcttgtt ttgaaagttc ggaaactccc atgattgaat cttcgatttt
    10081 ttgataattg aagaattctt caaaggatcg atatacagga actgtctttt tgcctacttt
    10141 tttctccgct tggacttgct gatttgccca cgctaattcg tgaatcaact tttctttgtc
    10201 aagccaagat aactgggctg cagtcatacg aatgttgtat tcatataacg tcattctttc
    10261 gatatctgag atattggtca tttccaaata tcgaaaagaa ttgataagaa tttgttcgta
    10321 tgccagtgca gaatctattc cgcttgttgt ttttccgctt ctttcaattt ctgattcagg
    10381 tttcggaccg ctaactttcc cgcgttcgac tccgccaatt cttttaggac ttcatcaaac
    10441 aatttttcga tgtctttaac ttcatcgatg taatcatcca tttcatccaa cgtaatagtt
    10501 tcttcttctg ttctatttgc tatttctaag actcgtgaca atgtgttggc attataagaa
    10561 cgtaattccg gtaagacttt tgctgagagt cccattccga attccatatt tccatcgatg
    10621 aaaggcatca ctttgtctaa ttcacgtaca aatttagtgc caaatttaaa cgaatattct
    10681 ttacctttaa tttttaattt caatgttttt catcctccta aaataaaaaa gagagcatct
    10741 aagccctctt atgctcctgt cgaagttgct ttcacggtat ctttgaatgc atattgaaca
    10801 acatcggctt gatcttctgt caaggttgca taaccatctt gaccaacacc atttactgca
    10861 aatgataaac ttaattcaac gttatcctct gcagcagctg atggagtaaa ttcagacaca
    10921 tatgcttggt aataagtagc tttgtactta tttgcatcat catctgtgcc ctgttctgct
    10981 ttgttgattt cccaaatttc aatgatatcg ccatttaata aggcttgttt catttcatct
    11041 acatgagaat cgcctttggc aactattgaa gtagccgaaa aatcatattc aactgggctt
    11101 aaactttgaa cgtttccgtc ttttgtcact gtagagtctg aatctcttga taaaccattt
    11161 tcgtgttctg tttgaaatgc cattttccaa gcagcttcct gagtttcttt tttcaataag
    11221 cgataaagca aaatgacatc aatacctttt aatgcttcca tgttcttcct cctatctaat
    11281 tctaaattca agtgtgacaa ccgctcgttt taggggcgta ttggttgttg tgtcgtccat
    11341 cacttgaatt ccacttgctt gataatttaa atcccaataa tagccttctg tggcttgtat
    11401 ctgcaacgct tgattaaata gtgccgaagc catatcagac acttgtttgc gttttttctg
    11461 caatccccaa acggaaattg tcaaattgac tgttcctttg atgtcggttt tattcggatt
    11521 gtggactgtt tgggagcttt ccaactcaac aaacggataa cctacatcgt ctaatggttt
    11581 gtagtcgtaa gtttggtatc caagagctag gctcctagca aacatttcat caaataatga
    11641 ttgatctctt gttttaatca tttgaataac ctctgcatgt ctttcataaa gattgctttc
    11701 tgcacattaa atgcgggacc aacgaaaggt tgagcgctca tgaatcgagt tccatattct
    11761 aagtacggac tgtaatcact ctgcggtcca acttgtgcaa caaaaccgcc ttccaacaac
    11821 cacattcgaa ttgatcgtct agtgaatcct gtcgggtgca caaacacatc cccttcccaa
    11881 tgtccattaa agcgggcttt ttcttgcatg cgcttagtaa gctcggctcc atttgtttta
    11941 actaccattt gcacatctct aagcgtgaca gcttcttcca agtgtttaag cagctcatcc
    12001 acacctttgt aacccaatga aactttcact ttgcaatcac ctcctggacg ataatgctat
    12061 tcgttgataa caggctgcgt tgagcgacat ctttgtatgt ctttccatta aaaataatgc
    12121 ggtcatattt tggaacaaca aaaagaggca tggttctgat aaccttcgcc ccttgtcgta
    12181 tatccccaaa aatcgccaca cttctatctg ttcctaagtc cgttacattt gcgtttgttg
    12241 ttaccgtaat cggtgcttct tctatccact cgcctaattc tggatcgtaa cgatttcctt
    12301 ctccatcttt ttcaaagata acctcatcta aaaatctcat aagaaccgca cgctcccttt
    12361 tctaggtttg tacaattctt cgtcatcttt ccgtttgaaa tcatcaattt ccgatgcgta
    12421 ttctgaaaaa tcggaatcag ggaaagccat cgataaaccc tcttgagcgt atgaagtcat
    12481 gccctcttgc ccaattcggt taaaacgttt gttcgttacg tcaaaaacga tgtattctag
    12541 tgattctggt gtaacagaaa cgcctagcaa tgacgctaag cgctctctag ttcgtctctc
    12601 gataacttct agcttatcat ctgtagaacc gcttagaagc ttcttaacgt catctttaat
    12661 taccattcgt acctatcctt cctacggctc agtcagtgtc aaaacgtgag tatctgtgtg
    12721 tgatccatct tccgttttta ttgttgtcgt gtatttaccg gctggtactg tgtcagtcca
    12781 agtgatatta ccgctagaag aaaccgtcaa tccgcttgtt actggcgcaa ttgaatacgt
    12841 cactgtttta tttgttgcgt tttgtggtgc aacggtagct gttaattgtc tattgccagc
    12901 agtccccgca actgcgcttg aggttttagg cgttacagta acgcctataa cggggattgt
    12961 tactcccccg ccggttcaat tttaccgaac gcagcatctt tgacgatcat aaagccgaca
    13021 tccattgttg cacgcaacgc aaccagctct tgttcaaaca agtttacagg agtgccatct
    13081 tcgtttttaa gcgtagataa ttgcgcatct tctgaaatct tgaacgaaat gttgaatggg
    13141 ataccataga acatatagtt aaagtcacca gcgtacaatt cacctttacc caatgctttc
    13201 aagtcaacca caggaagacc atcaatcgta ttcgccgagc ggtcatagat aaattcaacg
    13261 tttgtaccga ctgtttgagc ggcagaacgc aattctgtgc ggtttttacg atttgagatg
    13321 aacgcatttg gttcaaattc atttttgcct aaagcatctt ctaatgccaa aatgttgtca
    13381 taagtcaaag gacctttgat aacattagcc gctgcaacga cagattcttc taaagactgt
    13441 ggaaacggat tgtcagtgtt tagtaatgca gccgcatcaa actttttgta gaaagcttca
    13501 gcaattttag gttgcataac agtgaagaaa tctgacattt tgtagtgcaa gtattcacga
    13561 gacacaggaa taatcacgcc tagcttttta gctaccattg tcgcttgcaa ccatttaggt
    13621 ttagatgttt gaatcttctc accttcacca acccagtatg cgccaggacc ttcagcaaag
    13681 tattcaaatt tcttttcctt atctgtcatt tcttcgtatt tcgccaattg cattactttc
    13741 gagttttcca taatgtcgtt gataatcaaa gtattatact tgtcagggat tttaccctct
    13801 ttagtttcat aaactgttac gttatctgga ttccatgttt gtgccatatt gtttattcct
    13861 cctatttgat aattcgattt ttagcagcta gtttagccac tgtttcttta gtatttttcg
    13921 atgctgtaaa ttgtccacct tcatttggtg gtgtttgtct tgcgttttct ctcttaatca
    13981 aagaagcaaa gttagtgatg actgctacag cttgttttgt ggcatctgca tcatcagaaa
    14041 caatcagacc aagtaaatca tcatcgtgtg gtaaatttgc atctgtcagc attttagaag
    14101 cttctttcgt catttcagat agtgcctgtc cacgctttaa ttcagcgatt tcagcttctt
    14161 tttgttccaa ctcatgctgt agtttttctt ccgcattcat ttttgccagc tttttagctt
    14221 cttctttttt tgcttctagt tctttttccc acgctgcttt tgctttattc gtctcagcag
    14281 cgatcatttt cgctacttca tcacgagaaa atgttttgcc agtattgttt tcttcttttg
    14341 cttcggtcgg tgtctcttgt gagccagctg gtaggtttcc ttgttgtccc tcatcaccag
    14401 atccaccatc tcctggttca gaaaaaaatt gtaagttcat tggcataaat aaacgttttt
    14461 tcatgattaa tcctccacgg ttacgccgct acccgataaa tttgaccagt tacgccggtc
    14521 agtcggaaca gctcaattta tattgccccg agcagtagag ggcataataa aaagcccgtt
    14581 attcggcttc acttacatct cctgaaggta aatatttcac gctgatatca atcgattgaa
    14641 taccgtcttc ccattcacta tcagtttcaa cggatactct tagcactttt tcgttttgta
    14701 gaatcttatt taaacttgcg atagctctaa caattgcttc ttcattttcc atatgcgtac
    14761 tctcctctac atagaagcgc tttggaacac gctttgattc atattgtttt gcaaagcttt
    14821 gaagtactct gggttagact gaaccaattc ggttattgat tgcacgacag gttcaataac
    14881 ctttctagcc gtttcataca cttcttttac gattggttct aagacatcaa ttaaatgctg
    14941 tttaacggca tcatagtctt ctttaaattg agcatcattt tgatacaaat gcaaaatttc
    15001 ctctttgctt ttcccatatg ccgtttctaa aaatctgtca gatataactt gaaatgaatc
    15061 tttcttcatt gttataacct tctttcttta agtgacttct caaaagcatc acgatcaaca
    15121 atcggataag tagaacatct atcaaaagga tgtactggca tcatattctt acctacttcc
    15181 atttcactca ctaaatatgg accgtcagcg gcaatgctct tacataatcg gcaagcatta
    15241 ggttcagtta cccatccata tttctcaata ttcgcatcta agtaactttc cctctgtata
    15301 ccgctttgaa ctcttgttgt ttcagttacc attaaacgtt gcgtattaaa cttagtattt
    15361 tctcgtcctt cttctgttag aaacttactt aattctggag cgagctgttt aggattacgt
    15421 cccatcgtca cacttcgaac aagcaactta tccaaatctg ctttcaactc tgcttggtac
    15481 atccatagcc tttcgctgaa cgttgcaaat ccatccgctc gaaacgagct gtttatcact
    15541 tgctccacta attttgcata accgctttta gcaatcgcca tttctaagat gccagcttgc
    15601 cgcttaagct cgtctagccc tgcttttgta agttcattcg agaaatactt atctagctcg
    15661 ttaaacgtag ctataagctc taaaccaata ttggctttta acaattccag tcgattgaca
    15721 cgcatcgtta gattgtatag cttcaattct ttgttcgctg taggtgagaa gtctttctct
    15781 ttaacatact tcttagcctt tctagcaaat gcttttacat ccatttcatt agcacgcttc
    15841 attgcttcgc tacgagtaat tttctgcccg tttgaaaaac tattccattg tgcgtctatc
    15901 tctttctgta tcgcatcctg cgcgtattgc agacgcttct tgatctcatt catgcgtttc
    15961 ttgtcatctt taatctgttg ctcttgccaa gctttctccc ttttgatgaa gtaatcttgt
    16021 gatttcactt aatcactcct cgatgatttc gtaggtctca ataaagatat ctggtttgca
    16081 cggataaaat tcaccatgaa cgcctttgat gatgtaatca ccatcagata tttccattgt
    16141 gccttctaat gtttcaatac caggtaatcc attgggcata ttattatggt caatccatgc
    16201 ttcaccaacg aattctttgc atttgcgata tgaacgtgca gatagcattt taagttgcac
    16261 aatctcgaca gtaactggtt ttttacgtgc tttcatcttc ttcaacctcc gtatcagttt
    16321 ctttgtcact gtcaaacaca cctctgtccg ctgacgtttc agcattcaca cgttttaatt
    16381 ctgcctgtac atctggaaca aaagaagcga gtcctaagat cgtctcttga ctgagttcag
    16441 ctccagcatc aaccaaagat ttcaactctt ccagaatggc tttaggtaga ttaggcgtaa
    16501 atattacacg taagcctttc aaatcggagt tatccatttc agaaatactt gattttaggc
    16561 taaacaaaag acgataacgc cgcataagac cctttttgaa tagcctttgc tttgttgccg
    16621 tcatttgttc aaatccaaat aatttatatt tcatcgcttc tcctgattgc actccggaaa
    16681 aattgtcatc agtaagatca ggaaccattg agatttcgtg gatatccttg cgtactctgt
    16741 ctttgtatgc ttctacaccg ttcacatcat attgtttgta aatatatcct gcagtcacac
    16801 ttgttttatt accgttcaca tcagttccag attcaagcaa aagcatattc gcttctttct
    16861 gcttgatggc gtcctctgtg gatagtcctg ctgcttcaat atcaccacta ataactagaa
    16921 gagcatcgtt tagatcagtc atatagttgg cggtatcaga ctgccctgca tcgtatagat
    16981 caatcaaaga tagtacatct tcatacaagc ccatccgaaa acgattagga gaatactctg
    17041 taataggtac ctctttatat tcatgcggtt catcctgggg attttttaac tcaattgctg
    17101 ttagtgtcgt ctcatcataa gtgatacttt tttcttttgt gtatacgatt ggttgaatgt
    17161 actgtttatc agcatccttg ttgaatcttg tcttaggata ccgtacagcc aaaataggct
    17221 ctcgttttac tgtagtatca tatacaacaa atgtttcaaa tacattagcc aaatcaacat
    17281 aatctgtatc atctgaatct cgatagataa tctcataggc tctcccatac ttatccatat
    17341 caagccagag ttcagcattt aacccatcta tgtcattatt agtattaaac tcttcgattt
    17401 ctttttgttg atttgtatcc tcgatttgca cttttatggg attgcctgtg ttgtacccaa
    17461 catcaaacgt acaaagaact tttccaaagt tatgtgctga tcggtgatcc gctttttcct
    17521 tttctctacg tctacggtta tccatgatat ttgtatttct agctttgtaa taatcatcta
    17581 atacactaag ccgctttacc tgatattcat gatgatgttt tatcattgct gctaaagtat
    17641 ttgaatcgtt aagtaaatct tctgctgagc taaatctata gtgaagattt gattctacgc
    17701 taaactttac ataatttgtg ttcacgtcgt tcgaataatg tatatcagta ccgtattcaa
    17761 attcgtttac cttatccatt tctcacactc cttaaaacat acgtttaaca cgtttaatct
    17821 tttcgttgac attaactttt ggtttcttaa gaagctcatg agtatatatc gcatatcgaa
    17881 cggagtctag cacatcatca tattctttta ttggctcacc ttttttcttg tcccaaatgt
    17941 attgataaat ttcatcccga aatttcatca ctctatcttg acagataaac aatttatctt
    18001 gtttaaatct cttggcgact tcctcaactc cagataaccg tgctttatga gcatttttag
    18061 cattaatctt ttctcttaca aacctagcca catgctccgg tctcgcagag tcacaataaa
    18121 aaggcactcg taagccgtaa cgctcttgaa tgccttttgc tatatctacc caataatcaa
    18181 tctcttcaaa ttgagtagca tgttcttcga ttaaataagc tgttccgtca tccgtttcac
    18241 ctataactac gattgaaccc cagtgttcat atccccagtc aacaccacaa taaaagttgc
    18301 tcaaaggtgg taagtctttt gactggatgt agtgcttgct agcatcaaaa tcacgataaa
    18361 cgacaccttc tgcagaaacc cataaacctt tgatatcacg atcataaaac atgccgcttg
    18421 gtgttgattc tttaatatta tttctatagc gttcagataa aaaagtatta tcatccaatt
    18481 caaagtggaa cgattgaatg tttttacttg aattgtcgat atactctttc ttcagccagt
    18541 gttccgggtt gtcagggttt gtgtcagcta ggattctcgc tcctgttcct gaacaacgtg
    18601 aaacgatttc agcaaatact tcttgtctag ctagtgatgc ctcattgata taagcgccat
    18661 atgctgtcat accacgaatt gcaccaacac cgccaatgtt tcctgtataa gcttgtacga
    18721 ccttaacgcc aaataactta aagtttccat gtttatcgaa tttaggttct atgctgtaca
    18781 tgttgtatag ttcctgtaag atgttctttt ggattgttgc acttgaaact cctgctagga
    18841 tatacattgg ttccttaatg tcttcttcat cggcaatctt tcgcacacgt cgcaattcaa
    18901 acaagaacaa atcattgttt atttttgttt tcccagaacg ttttgctcca tgtaataaag
    18961 taataaacca atcattctta atggttttat ttaacacgtc gatttgcttt gggttataaa
    19021 tatcaactag tcccatctaa ttcaccacta atcttttcta acaactcatc aagtttttct
    19081 tcggtagaac gctctgagtt tatctgtaat tgttctaatt ttgcttgcat taattttatt
    19141 tctgtttgag ctttttctaa ttgcgtttgt gataacagcg atttatactt caaatacaaa
    19201 tccaaggctt tcaatttgct ttctaaatct ggtgtatatt catattgcat atgtttaata
    19261 accttgtttt tctccaagcg gtctatttgc ttgctcacgc ttatttgtac ttctccttgc
    19321 caaatatcta tcaattcatt taaagcgtcc tcagcactta atttgcgctt cttttcgata
    19381 ggatttagcc gttcattaat atactgtatt atgttaggtt tagttaggtt ttccttacct
    19441 attactcttg ctgatcgttt gctataacct gctttaatgg cggcttgagt agcattacca
    19501 ccatttttta tatattcatc cgcaaaagct ttttgttttg gtgtcaaatt taccacattc
    19561 aagccaccac ctttcaattt tattaacaat atctctcaat atttgtctgt atgttctgct
    19621 cactaaaata tccatggcca cagtaacgaa gattgtactt atcgatctct ttcggtgtag
    19681 cttctctggt catttcaatg atggagtact tctttttgat ttggactgat tggacaactc
    19741 taattggatc atctgcgttt ggttgcggat atttatttga taatgataca taccagtagt
    19801 ttctcattat gtatcactct ctctgttggt tattggcaga aaaggttcgc atcataaatt
    19861 ccatagcctg tccttcatta aacccttgta gaataagctg atcgtagaaa tacttagctt
    19921 gttttgcgat taacgctaag cttttttgag tttcatagaa tgtcgcctcc atggtttggt
    19981 ttgcttcatt cgttttaaat aactctctta attggttttc attcatagat aaaactcctt
    20041 tcaaaataaa aagaccactc aatgagtgat ctaatatgta atacaggacc cactatcctt
    20101 cgagggaatg tggattacag aaacatcagc gcgctatagt ttccaacctg tatattcggt
    20161 tttactgaca ggcagttatg gatcaccgta aaccaaagtc actggcaatg aatcgaacat
    20221 tgcaacacca ttttccgata ttttacagac tctccatcat aaaaactctc acctctagaa
    20281 ttgtctatct ttaggggatt tatttcaaga tgcctcttgt cctttaccct cgacggtctc
    20341 ttgcctagct gcataaacta agctcaggtg ctcattacca ctgatttaag ttattagcgt
    20401 tacctttccg ccacagtgac aaatttatat tgtgaaaata aatactaagc gtataatttt
    20461 aattatcagc gagtggtccg ctgaaataaa ttataggtgg tgaaaaattt gtactttgtt
    20521 ataagagaag caactaatgg ccaatattac ttcgttatta agtccaataa caatgaagta
    20581 gtagcaacta gcgaaacata cttgactaaa tattccgctg aacaaaccat taattctata
    20641 aaaaacggaa tcaccaaaga ctctcaagtt atcgatatga ctaaataaga cgactaactt
    20701 catttgctag ggagttcatt tcagctgctt tttctaccaa agcagttgag atactttcta
    20761 gcttttctga tttaacagaa ttaaatactg gcgtcttgat actaatcgat acatcttgac
    20821 gaggatcagg actcgttgct ctattttttt gcaccactga tctgtacttt tctactgaat
    20881 gatactcaat tacttgttct aaatgttttt tgtactcttt acatacagct tctttttgga
    20941 tcaattctaa acgttgttgc tcaataatat caatcaaccg ttttcgatcc ataccttgat
    21001 actttatttg ttcgacttgg tcactagcct tgttgagcag ttcttttagt tcagtcaaat
    21061 ctaatttgac agttgctttg atttctttat ccattcgttt tccctccaat acataaatta
    21121 atagacagca acggatgata gataataaga acaatcagaa ggagttgaaa ttcacatcct
    21181 tattcttaat atttccgttg ctgtctatcg aagcttaatt gtgaaacaat aataaaacga
    21241 tgttcctttt attattattt tgtctcagac ctatcactaa tctttcgaca ctaccataat
    21301 atcacgttaa aacactcaaa aaccctacac tatccctaca aaaaccctac aaaatcaacg
    21361 atactgaact aatacgcctt ttttgtatgc ttcagcaaat tcgatcaatg cgatggattt
    21421 cagcttctct acattcttct ctccgtatcc tcgtatcaat tgacctattt cataattaga
    21481 gtgcttattt acgtcacaga agctgtagta gagtatctga cgactaatca gactgagagc
    21541 catcaaagcc gctaaaatcg cgtctctctc tgcttctata tccatcattt gaatgatcgc
    21601 gtcttctgcc ttattgacgt gctttggtgc cttcggcata tcggttatga taggcgactt
    21661 aatgtctatc aaagagcgac ctgccatccg ctccaagcgc cgaaagttct tcagcacatc
    21721 tctcgcatta catcttgtct gtttgaaatc tacctctcgt aacaattgca tcaagtcaaa
    21781 ccgctccttt atgtgatata ataaacttgt ggaatttatt agatcagtcg gagcgatccg
    21841 gcttttttat ttgtcattga ttagtttaat atccaccaat ctcgctacag ctaaattctc
    21901 tttgcttttc gctaaccact tgtcgcattc catcgtgttt tcaatacgaa tgatcgctga
    21961 gtgattataa acatgttcta catatccacg aaatggatag atgaatcctt ctgcttcaca
    22021 tcgaaccatg tcgccgactt tgaattttgg tttcttacgt gttttagggt tctttgtcgg
    22081 catatctagc attaaaccgc cgatgccgtg gctgctggag taaaatccgt cttttagttt
    22141 cattccgctt cctcctgttt taattttcac catttctttg ttgaaaaccc gcaacgtcta
    22201 tactgtttaa ttcgtttctt aggtctttct agatcaacca gaataaatct gtcggatata
    22261 tgataatctt ctattacagc cgtatacatt ggattcgaaa ccgagtacca tgatcttttt
    22321 ttatcaaact catccgcatc atctcttgta atttctatca tgttagcaga cattagatag
    22381 ctctcttctt ctgcctcgaa tgggcaggaa tattcatccc agcttcgagg aaacctggcc
    22441 aaatctcttt ttaaataaac ttgaacattc attccgcttc ctcctcatca tagaaaatca
    22501 ttccacccaa gacctacgct atgcataaag caaaattcgt tacacaatag atgtctttta
    22561 tggtattcat tcttaaaact caatctgttt ccacattctg aacaataaag tttcactttc
    22621 ttatctggat aaccgttttt gattaactgc tttttaaatt gtttattttt ttggcgttta
    22681 ttcattccgc ttcctcctgt tttaatcccc attgagcgaa tgctgctaga acttcaaatt
    22741 cttctttcat agtaaaatca tcataaaaac caaaaatatt cgtcggcata tcgtttgctg
    22801 catgctttaa cacataaaaa ggaaacggcc attgtgattt tgcgtaatat tcttctaacc
    22861 actccaacac gattttctgg ttgtcgttga gcgcttgtcc atgagtgaga actttacccg
    22921 attcccaatc tcctgttttt ctagcatatt catattgttc tgctgttaag taatcgctca
    22981 tctttctacc acctcttcca ctggcacagc aaatggccag tatctttcat caatttcttt
    23041 gatttccgct tctgttagct gataagcaga tttttcccaa gtacatagcg agcgactaat
    23101 atcaaaacag aaatcatttc tgttattaaa tttcttgata agatataagt caccaataat
    23161 aacttcatac aacggttctt tctcaacctc gtagccgttg tataggctca atagtgtttc
    23221 atatgattgt ctcgttgccc aagcgagtaa ttcttctcct tgttcttgcg tcaatatgcc
    23281 atctcgcaca aaccaatcac agaaataata tgaatcacca tctttggatt tgattaaata
    23341 cgctattttt tcagcttttg ttaaattgtc ggtacttttt tcgatccaac ctgccacgag
    23401 ttgtggaata actggtttct gcgattcatt tagttgttgg attagttcta atgcgtgttc
    23461 taaacccgca tattttccag catgaaaagt acttatacct ttaaagtcct ctatgctacc
    23521 tacaaggctt gatagtttat caatcaattc ctgtttattc atcgctgttc ctccaataac
    23581 tcgctattct cgtatatatt cccgatgact tccaaaggtg aatctacaaa gtacaaaaaa
    23641 tcaaattccg tgccgagatc tttgtaatca acacaaaatg cgccttcttc aaactttatc
    23701 gttgaaaaag ttactttcaa atcagcatcg agagttttga caatatcccc ttcaaatata
    23761 tccacaccat tcttatcttt cattcctgtg gattgcatga gttcaatatc aagaaaatca
    23821 ataatttctt caaactgctt atcttcttct aaatcttccc tcaaaactaa acaattgcca
    23881 taaataaaat taggtaatgc ctcagcaatc accccattaa gatgattgct taaaatatca
    23941 ggttttaccc acgctctaaa cttcggtatc attcgctgtc ctcctcgtat ttttcaatca
    24001 attccattac ttttttcact atttcaaact caaccgcttt tgattcttcg aaatcatgaa
    24061 caatttctgg aaacagtaca tcatcaacta cccacaaaat aatctggcgc tttccaccaa
    24121 aatttatgat tagatggtcc gattccacag atagtgttgc tcctgattcg atatcatata
    24181 aatccatgct gaattgaacg agttttttta tcatttgctg tcctcctcaa atactcttct
    24241 aatatttctt tatacttctc tacaaatttg aaacgatctt gatgaagttt cttgctccaa
    24301 tttgtttgcc gatccagctc acgcatctga tcgaaccctt tttgaatttc gttgtaataa
    24361 aattcaatgt ttgctgctgc tttccaatgc ctgctacttc gcactcctgc tcctgtttca
    24421 gccatttcta acttaactaa ttcagctcgt tcttttgatt ttttgtcttt ctgaatcttc
    24481 atcatgattt tcttgaggat gatgtcactg tattgtgtaa tgagatccat tatctctcct
    24541 ccacatacct aaactgtcgt ccttttgaat caatccataa gctcctagct ctatcccaga
    24601 taatgttttt gcttaatcca gtaatttcag ataactgttc agctgtacct gttactagaa
    24661 ttcgatcacc atgccagatt gcaatttttc tcggcgtttt ccgtttaggc ttttcagtcc
    24721 acattgattt accgagcttt tggacttctg caactatttc tttgtcttcc tgccaagatt
    24781 ctgacttggt taattcagca attcgtttca ttgtcgcttt cttatccacg ctcattcctc
    24841 caatctacga atttcccttc ttaagttctc tatgtgcaaa tcgattgcct tcctcgccgt
    24901 ttcattgacc atcactgcct ttgttcgttc cagatcgtca atctcacgct gaaggcttcg
    24961 aatacgcatt tgaatcactt cttctgttgt catgatggac cacctcgcta aaaacgctct
    25021 tccttgaacg tattccgata ttttttagct aatatcaacg gcgcttgata ttgatgacag
    25081 aacaactttg ccttgatctt aaagtctttt gtctgcattc ctttgacatc tacgactttg
    25141 acaagtttgc cgtttttata aaatgtgaag tcgggaatat actcgatctt gcgatacttc
    25201 tttccgtcta gttcaaattt cggcatcagc tcaaatcttt cctgaagttt tactttccag
    25261 ccatttgctt cagcttgcca caaggctaga tcgtaatact ctgcttctgc gatagaatca
    25321 aacttgatac ctcgatgaac agttttttta ttacggtatt tattcatgcg atactacctt
    25381 tcactggttt tatgcgcttg tctgctgttt gttggaattt cagcgcataa ccttctgaat
    25441 tcttaaatat cctagaaaca attctttcac cgtaggcttc tcttagttca ggaccagata
    25501 agtttgttgt gatgatcgtt gccttgttct gtctggcttc taagagcgtg tttaacgtgt
    25561 tgtttgtaaa ctgcctacta tttgataccc cactacctaa ttcagctcca atatcgtcaa
    25621 aaaccaccaa atcagttgtt ttaatatcgg ctataagcga tccttcaatt tcttttctca
    25681 gttcagtatt gttataagaa aactttattt gctctaataa ttcttgatag cttataaaaa
    25741 gtattttctt gtcataattt gagcgctcaa gtatttccca agctgtggcc attgataagt
    25801 ggctttttcc gcttcctgat ttccctgata gaatgaaatg tgcaggatgg ttcagcagaa
    25861 cctcattcgt atagcttttt gctttttcta aagcgatttt tgtttcttga tccactacgt
    25921 gataattttc cattttgcat ttaaacaaag ttttatctgt taataccgaa ccattttgaa
    25981 aaaaactcaa cgctcgtgct tttaagctat cgttatatat ccgttcggtc tgtatatcct
    26041 ctttcacacg taacgcttta taaccacaac tcatgcatgt tggtttacaa cgttctgaac
    26101 catccttatt tttagctcgc caactataca aaggttcgct acattctgga cattttccgc
    26161 tttgcactaa tactcttctt attagtttct ccatagcatt tgctaggctt tccatgtgat
    26221 gcatctcctt tttaaattgg caagtcgtca tattcactag gattgctgta ctgtagtttt
    26281 tgactttgct ttttatgatt cttcttgtct gctttgattt cgaatttgag cttctcaaat
    26341 ttttctctca atttcttagc acttctaata tttccaaacc aaaattcatt tgtaggtagc
    26401 caattgatca catactcaat cgcttctata gatgctttat ctctttcttc catcaacctg
    26461 attgtgtctg cccatttttc gatatctact ttgttcattt cttttggaaa atcttcagtt
    26521 aaattacttt gcaacttttt agcaaggcgt aagtgttcgt cagaatactt acctttcttt
    26581 tcttctttat ctatatcttt atcttcttct atatctttat ctgtaccgtc acgtgacgtc
    26641 acgctaacgt cattttccaa tttgagacgt tcctgtctct ttctttccct gtatttacgg
    26701 tttctttcag cattttttag ccttacttta tccataccct cgatattttg atgtttttcc
    26761 caattactga tggcaattag tccatcactg cttagatcaa tcatgttgaa atttgccaat
    26821 gtagttagcg ctaagcgaac cgtatttacg tttttgccaa acaatgtagc aagcatttct
    26881 tcggtataag gcatgttcct ctggatatat atcagaccat cgtcgttagt ctttcctgct
    26941 aaaactagta atcgaatcca tataacgatg atggcatccg actcaggaac agcttggatt
    27001 aatcgtattt tttcatcgtc aaacatagta gttttaagtt tgatccaact tatctcagcc
    27061 aaatttatcc tcctatcctt aactttttaa ttgtttcctg gtttaacttg atccctttga
    27121 tttgatattt atttttgaaa ttgatcacac ctattttgtg tttctctgtg tgatggattc
    27181 tgcagagtgc tgcaaatgtg tactctgaat gatcaacttc tttgcgcttt cgtcttccca
    27241 gcgctttgtc aaagtgatcg atatcagctc ctgttttgcc acagatgcag cagactcttt
    27301 ttgtaatgca tttgtagaag taatactctt gattcgctgg taaaatctca tatccttctt
    27361 tgaaaggaat atgatgttca aagatgaaat ctaggatgat attcgctaag atattggcat
    27421 cactcacggt tgtattcgat tcgtctttca ggcttatttt gcgccctgtg acaccttcaa
    27481 aacgaaagta gaagaattcc ttccagaagt ccgttggcat gcctgtatcg ataaaaatat
    27541 cgcctatcag cgcatagatg aagtttcgtt gctgtacggt gaaacgtcta ggatcaataa
    27601 aacgaatttc aatgactcga tcaccatcgt agccgtcata catcgtcttt agtcgatcaa
    27661 tgttcacttc ctcattgatg gttgcgctta tgtcttttcc tttgaacttt ttcagaaccg
    27721 ctgaatatga atcgattaat ggtttaaaca ctcatatcac ttcttatcta attcttttct
    27781 cttagctgct attgctcgct ccatcaaggc acattgctca tagcttaact gttcaatagt
    27841 ttcaacgtta tcagctaaga gccctaattt atctgtctgc tcattaacat attcgattaa
    27901 ggttttggtc atatctttac ccatctgctc attgaaagct tctagaatcg tctctagcat
    27961 gtttaatttc tttgtatcga ttctaggtgg tgttggaata tcttcccctt gaaatacata
    28021 taatcccagt ccgtgtagag ccaatgcttt cacaaagcat cgcttcaatg agttattgat
    28081 ttgcattgca tttggtttaa caactggttg gtttcgataa tctaaaacag gaaataattc
    28141 ggtttccgtg tgtcctttaa ccgttactga gacagataca taagtcccag tttcatccat
    28201 aagaaaaggt ttatattcct caacaagaaa gtcttgatga gttccagaaa caaccctgta
    28261 gtgtttgtac tcattaatag ttaccgttgc ctgtggatca ttctttttca taatctccca
    28321 cgcgtgagcc caagataaat aatcaaaatc tccttttttc ttgagaattt tatttaactt
    28381 acgactaaaa agtttttcaa agttcgttgt ccctttgatt tcactcatca aattctgcct
    28441 ccatttcagc aatgtatttc ttacctggtc cgtaataaga gatatcaatc aagttatctc
    28501 tgtcgtactc ttctagcgca tcaatcaagc catcttcgat gacatagata tactcaggtt
    28561 ttttggactt cctcgataaa tggataagat agacatgatc ccaaatactc acaaaatttc
    28621 ccaagtcatc ttgatcacat gctagttctt catccgtcaa gagatttcgt ctgatttttc
    28681 gattatttgt ttccttgata ttcgatttgc cccaactagg atcagtcaaa tattgatcta
    28741 gagtggaaag ttcattttcc atatgttaaa atctccttag ttatgatttt ttgagtgact
    28801 cattgctttg gtcggcggag tcactttttt atttgttgcc atgctttttg cttttcgata
    28861 tgttgcttgc ttaaaataat aggacggcta tttgcccacc aattatcagc aatcacttta
    28921 ccgattttta gcgcttcttc tcgtgccata gttgctcctt tcttttgaat caagcagatt
    28981 gattaaaacc atcaatgctg cgaacaaact tcccccgata atactttggt gtactactat
    29041 cactaatagc cctaggatga atcctataaa aagtgtgtct gtcttcttca taatctaatc
    29101 tccctatttt ttatttctag cattctcaaa tcctcaagtt cagaagcgat tagttcagct
    29161 tgtctatctg atagctcatc ggcttttcta agcgcttcac gatcatcttg taattgtttc
    29221 ctgcgttgtt taatcaaacg gagaatttga tgttcttgtt gcaatgtgta ggacataaaa
    29281 tcattctcct ttgcctttag aactcaaagt tttctttcaa aaatctttgg agttccgatc
    29341 gttcaattct gatgtctaac ttgctccact gctgtgtttt taagcctagg tttatccaat
    29401 gtgttaattt gtcatcacca attcctaaaa cttttcttac ctctgatttg tttggatatg
    29461 gaggaagctc tactgatttg ttcataaggt gcaatcgttc gtccaatgaa ttaagcacag
    29521 cattcgtaat ttgtgtagtt aattctgaaa ctactaaatt atctggaatt gttatttgca
    29581 tgctttttct ccttttctaa ttctgctagt actgcctcaa ttggcttgat ttgtttatct
    29641 ggttttctac gcccgttcat aatatccgac atataggctg ttgaaatatc aagcttttca
    29701 gctaaccaag cttgactctt gttatgcgta gctagcgcca cacgcacttt taaaatgaag
    29761 tcctgcgaca taactatctc tcctctaata gatcaatttc tggaatatat ccttcttttt
    29821 ttagcgactc ataaatgaac aaacgtcctt tttgagtcca ttttgtattc atcacaactt
    29881 ttgttccacc atcagatttc ggaatctcag ttgtatgaga ttttgtatat ccttgtctca
    29941 tatgtttctt gcataataac cattggttgc ctactttttt ctgaatacct agtttatgaa
    30001 gtagtttgtt catctgttgt ggagacatcc cataatctgc tgcaatctga ctaattgtta
    30061 ctgaatctgt agaagataag atactatcca aatacgaaat tttgggttcg tattctgcaa
    30121 ttttttgttc tgcgattagt cttccagtac gttcttcttt caacttagtt gctaattgga
    30181 tgattgtatc tggattaagc aaggcttctt ctactttttc tggagttaga taacctccat
    30241 gttttctaat tgctggcaac acttcacttg ttacccatcg tttgaatttt ttggcagaag
    30301 gaagttttga ttttaagatt aaactgtaaa ggcctgactc gttgatgatt gtcatattac
    30361 gattttgacc tgatgcacta attcggtgca ttagcctatc ctcttcgtca acatgatttc
    30421 tgacagcgtt gtctgcacgt tcgtatccaa gaatctcagc tacatcttta cctacaaaat
    30481 acggttcatc atttaataaa actgttctta cttcctgttg tccgaaatta aaaatttgtg
    30541 gtgtgttcat tttgctcatt cctttctttg gtataatttt gaatagaaag cgaggtgaaa
    30601 atagtatgga agaatttaat atggatgtcg acgccttatt caaacaaacc gtattcaaga
    30661 ctgttaataa agaattcaaa atagatttcc aaaatgacga atcttttcca acagaaattg
    30721 aattattcga agaactttct cagagcgttt ctgagtcttt ttctcgccag ttgcaaaaga
    30781 atttttttga tgctctcgtg gatgaatttc atcaacaaca gcactaaact tttggtcttt
    30841 aaaatttaaa gttattaaag ctccttttgc ggaaggagtt tttttatttt gtttcatgat
    30901 gtttcctcct tttctttaaa tatgtaagct aacaaaatta gctaattttg ttgacagttt
    30961 ctacaaaatt ttgtagaata agtgcatagt taaataagca cagaattacc ctataaatta
    31021 acattctaag tttcccgacc ttgaatttgt ttactttatt aggtatcttt cttattgctt
    31081 gttagcttat taaattagct tacgaacata tattactata aagttttgta gatgtcaacg
    31141 attatctaca aagtttttaa gatgtgtttt aggcatgatc ggagaatcat tattatgaca
    31201 acatttgaga gagtaaaaat gttagcagat aaacgcaaaa tatctattgt cgaattggaa
    31261 gaaaaactta attttagtaa aaattcactt tatgcgtgga aaaagagtaa gccatccatt
    31321 gataaactaa atgcagttgc tgactatttc catgtttcaa cagattattt gctaggacgc
    31381 acagatgatc ctaacgcggg agttgcacca gaggaaagaa aactaaccgt ggaagaagct
    31441 ttagcatctg ttatgagtag cgacggaaaa ccgctcaccg ataatgatag ggaaatacta
    31501 tcagctatga ttgaagcata tttagagaaa aaagataact aaataagtag gtgagtcatt
    31561 tggacagtca aattgaaatg ataattaatg aactcggcgt taaggtagaa gagcgtgaaa
    31621 accttgatgc cgatggccat tatgttgctt gtatgaatac catagtaata aaagctaatt
    31681 tatctaagta tagaagacaa agaaccttat tacatgaatt aggacacgct tctaaacatc
    31741 atgataatta ttttttatat aacttagcat tctctctcca ttcaaaaatg gaatatgagg
    31801 ctgatcgctt catgattgaa aaattattag atagatatat tgcaaagtct gaattagaac
    31861 cacacaatat caattacatg aaatttatag aagataacaa tttaagcgtt cgcttcgaac
    31921 cacttgtgaa agaattatta aaagctcgca tctattgtta tgcagctctc taaaattttt
    31981 taagcaaaaa aagaacatat gttcaaaaat agaaaggtga acaaaaatga tatatacaga
    32041 attcaaagaa tggttagaaa aaaacacaac cggatacgaa acatttatcg tcaaagctac
    32101 taattatcaa attgaaaaaa acaaaaatag acccccaaaa aaacgctggg atgataagaa
    32161 aatagataaa gctgtattag aaatgtggaa acaagtcgtg actaacttgt atcaaacaat
    32221 tcgtaaagaa aaaggagttc cattaattaa cgggaaggaa atatggcttg aatttataga
    32281 ggaacaagga ctgatcgaat ttttcaatga tagcatggca gaattagaat ttgaataggg
    32341 gtaatattga tggcaatgat aaaacaatat aaaaagaaaa atggcgaaaa agcatggtac
    32401 tttaaaactt atctcggtat tgatccgcta actggaaaga aaaaatatac tactaaacga
    32461 ggatttagaa cacaaaaaga agcaaaaaca gcactttcta ggttagaact agaattacaa
    32521 aaaacaggaa tgcccacaag tacaaatact actttcaaag aagcagcaga attatggcta
    32581 gaaagctaca aaaaaactgt aaaagaaagt tcatattcaa ggactaaaat aatctttaat
    32641 aaacatatat atcccaaatt tggaaatatt aagctttcta aaattaatac ggcatattgt
    32701 caaaaggtag taaatgattg gagtgaaaaa ggaacttcaa agcagtaccc tcttttcata
    32761 aactatatga ataaagtttt taagtatgct ataaatattg gtttaacatc tgataatcca
    32821 acattaaatt tacttattcc aaagccacaa attaaaacag aaaagaaatt aaaattatat
    32881 acaaaagaac agttggaatt atttctaaat gaagtatctc aagaacagaa tccatatttt
    32941 aaaaacagag actatacgct ctttagacta ttagcattca gcggatgtag aatcggcgaa
    33001 atattagcac tcacttggga caatattaat tttaaaacaa atgaaatggc cattaaaaaa
    33061 actgtagctc gttcagataa atattatata tctgaaactc ctaaaacaaa aaaatcaaat
    33121 cgaataattt atttagatga aaaaactata aagcaactaa aattttggaa gctcgaacaa
    33181 agaaagtact tatttcaatt aggatttact aaagctaatt atttgtttac caatgacgaa
    33241 aataatttca caattaatca gtcagtggca gaaagataca atatatatcg tgagcgtgcc
    33301 ggcttacctt atatcggtct gcatggtttt agacatacac atgcatcaat gctatatgag
    33361 gcaggcgcag atcacaaaga agtccaagaa agaatgggcc acgcaaatat aaaaactact
    33421 atggacacat atacacacat tactaacagc aaaaaagaag aaacaacaca aaaactaaca
    33481 aattatatta acttctaaga aagtatggtc aaaagtatgg tcaaaaaaat cccttactta
    33541 gcaaaaaaag ctccatccct tgccacacaa gagatagagc agtttatt
//
LOCUS       DACEDD010000031        32162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEDD010000031 DACEDD010000000
VERSION     DACEDD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.031"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..908)
                     /locus_tag="IWO45_001616"
     CDS             complement(<1..908)
                     /locus_tag="IWO45_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ0333094.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYI"
     gene            complement(1049..3412)
                     /locus_tag="IWO45_001617"
     CDS             complement(1049..3412)
                     /locus_tag="IWO45_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0333095.1"
                     /translation="MATVILAEKPDQAEKFAFALADKKIPKSGGKFEFQSEVFGDTIV
                     TWGIGHLVGLSMPEKYEWLPNKEKWDLANLPFLPKMDELKYEVSKGKSQQFTVVKSCL
                     ENADQIIIATDPDREGENIAYNIFKLCKQSIFKKPMKRLWINSLVKSEIIKGFKNLRN
                     AEETISFFEEANARQIADYLVGMNYTELYTLKLQQKGLSGVVSLGRVQTPVNTLVVEN
                     DLSIKNFKSEKYKVIECHTVDREPKVIFKNSREYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            complement(3427..3645)
                     /locus_tag="IWO45_001618"
     CDS             complement(3427..3645)
                     /locus_tag="IWO45_001618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333096.1"
                     /translation="MELIPYVYQHLYKAKSMKDGNEVVGSLICCPPFSYIATIESMKK
                     MCVDELNDGEVKNLKLTRVLERSIEKFK"
     gene            complement(3662..4021)
                     /locus_tag="IWO45_001619"
     CDS             complement(3662..4021)
                     /locus_tag="IWO45_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ0333097.1"
                     /translation="MNIDGIYNESDFKKGDIAVIRSEYDFPSEIGIHTVEGETVCRYW
                     GRKGNLILLLDTDSGEKLKFCFWHNTNYCCEPIPKRLIDVNKESVRYLADGSRIRLTY
                     QKSENSSITRVRDIVLI"
     gene            complement(4157..4456)
                     /locus_tag="IWO45_001620"
     CDS             complement(4157..4456)
                     /locus_tag="IWO45_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0333098.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            complement(4690..6291)
                     /locus_tag="IWO45_001621"
     CDS             complement(4690..6291)
                     /locus_tag="IWO45_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0333099.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            complement(6688..6942)
                     /locus_tag="IWO45_001622"
     CDS             complement(6688..6942)
                     /locus_tag="IWO45_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333100.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            complement(7441..8325)
                     /locus_tag="IWO45_001623"
     CDS             complement(7441..8325)
                     /locus_tag="IWO45_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ0333101.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            complement(8312..8875)
                     /locus_tag="IWO45_001624"
     CDS             complement(8312..8875)
                     /locus_tag="IWO45_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333102.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            complement(9371..9679)
                     /locus_tag="IWO45_001625"
     CDS             complement(9371..9679)
                     /locus_tag="IWO45_001625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333103.1"
                     /translation="MKLEIKEVVCDWGIYVDGETYPFMIFNSKANAQEIMRIMELDNK
                     HERFDSVKKMENECEAVEYQKTCANNKYILGAMGLGKSASIKQVYEESQENCVYENSN
                     "
     gene            complement(10123..10734)
                     /locus_tag="IWO45_001626"
     CDS             complement(10123..10734)
                     /locus_tag="IWO45_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0333104.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            complement(10774..11556)
                     /locus_tag="IWO45_001627"
     CDS             complement(10774..11556)
                     /locus_tag="IWO45_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0333105.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            complement(11570..12952)
                     /locus_tag="IWO45_001628"
     CDS             complement(11570..12952)
                     /locus_tag="IWO45_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0333106.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYELYIKDFPEIPYKVFKD
                     IQEKYSDTFSLDKDYELLNPIIKFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTT
                     LTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNVELKNLNIGNNIKNIRKSKN
                     MTQTDFAELMGLSRSYIGDLENNRRNPSIKTLEAIARALDVPIVALINDSSTDNIVEP
                     YESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMIPNLKDVSKRIKYIR
                     KKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVGETTTDWIKWGTLEE
                     YITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDKDYELLDSIIKNIFTKYY
                     SKEFEVTQIP"
     gene            complement(13001..13897)
                     /locus_tag="IWO45_001629"
     CDS             complement(13001..13897)
                     /locus_tag="IWO45_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333107.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            complement(14092..14634)
                     /locus_tag="IWO45_001630"
     CDS             complement(14092..14634)
                     /locus_tag="IWO45_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333108.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYETKFCKRGD
                     CMKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERGFSLPNKERLIKIA
                     ELSNKTIDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDK
                     DYELLDSIIKNIFTKYYSNG"
     gene            14805..14993
                     /locus_tag="IWO45_001631"
     CDS             14805..14993
                     /locus_tag="IWO45_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ0333109.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            15039..15416
                     /locus_tag="IWO45_001632"
     CDS             15039..15416
                     /locus_tag="IWO45_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ0333110.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(16042..17214)
                     /locus_tag="IWO45_001633"
     CDS             complement(16042..17214)
                     /locus_tag="IWO45_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333111.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIMFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(17458..18426)
                     /locus_tag="IWO45_001634"
     CDS             complement(17458..18426)
                     /locus_tag="IWO45_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0333112.1"
                     /translation="MILENDKPYYVVIGGVNGAGKSTIYQIQNSKRKELDTDRINADE
                     ILRRFGGNWNKQSDINKSMREVLREIEGHFKEGKSFHHETTFGGNPYPDKKRLLNAKK
                     LGYESFLIYIGLDSPERAIERVKSRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            complement(18423..18620)
                     /locus_tag="IWO45_001635"
     CDS             complement(18423..18620)
                     /locus_tag="IWO45_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333113.1"
                     /translation="MRDMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLV
                     LSSYVTRVDQKKVINQKDFKI"
     gene            complement(18631..18948)
                     /locus_tag="IWO45_001636"
     CDS             complement(18631..18948)
                     /locus_tag="IWO45_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333114.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRLIEVNNLGIIEDYLAKLDEAVKQSIEKNGEDHVLTVVLKDLKDRLEAEAENF
                     NKD"
     gene            complement(18985..19203)
                     /locus_tag="IWO45_001637"
     CDS             complement(18985..19203)
                     /locus_tag="IWO45_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333115.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKANGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            complement(19312..19503)
                     /locus_tag="IWO45_001638"
     CDS             complement(19312..19503)
                     /locus_tag="IWO45_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333116.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            complement(19665..20021)
                     /locus_tag="IWO45_001639"
     CDS             complement(19665..20021)
                     /locus_tag="IWO45_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333117.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            complement(20046..21050)
                     /locus_tag="IWO45_001640"
     CDS             complement(20046..21050)
                     /locus_tag="IWO45_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ0333118.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            complement(21087..21347)
                     /locus_tag="IWO45_001641"
     CDS             complement(21087..21347)
                     /locus_tag="IWO45_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333119.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            complement(21356..22429)
                     /locus_tag="IWO45_001642"
     CDS             complement(21356..22429)
                     /locus_tag="IWO45_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333120.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            complement(22437..24263)
                     /locus_tag="IWO45_001643"
     CDS             complement(22437..24263)
                     /locus_tag="IWO45_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0333121.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATSEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKNDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            complement(24418..25284)
                     /locus_tag="IWO45_001644"
     CDS             complement(24418..25284)
                     /locus_tag="IWO45_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333122.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLIVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            complement(25459..25722)
                     /locus_tag="IWO45_001645"
     CDS             complement(25459..25722)
                     /locus_tag="IWO45_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333123.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            complement(25724..26053)
                     /locus_tag="IWO45_001646"
     CDS             complement(25724..26053)
                     /locus_tag="IWO45_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333124.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            complement(26075..27610)
                     /locus_tag="IWO45_001647"
     CDS             complement(26075..27610)
                     /locus_tag="IWO45_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333125.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            complement(27677..28144)
                     /locus_tag="IWO45_001648"
     CDS             complement(27677..28144)
                     /locus_tag="IWO45_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333126.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            complement(28141..28437)
                     /locus_tag="IWO45_001649"
     CDS             complement(28141..28437)
                     /locus_tag="IWO45_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333127.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            complement(28788..29018)
                     /locus_tag="IWO45_001650"
     CDS             complement(28788..29018)
                     /locus_tag="IWO45_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333128.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            complement(29054..29950)
                     /locus_tag="IWO45_001651"
     CDS             complement(29054..29950)
                     /locus_tag="IWO45_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333129.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYKNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            complement(29964..30614)
                     /locus_tag="IWO45_001652"
     CDS             complement(29964..30614)
                     /locus_tag="IWO45_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0333130.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            complement(30629..31765)
                     /locus_tag="IWO45_001653"
     CDS             complement(30629..31765)
                     /locus_tag="IWO45_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333131.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            complement(31787..>32162)
                     /locus_tag="IWO45_001654"
     CDS             complement(31787..>32162)
                     /locus_tag="IWO45_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ0333132.1"
                     /translation="INSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPKDSGSE
                     VADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAVVQTSS
                     EDTYMINFDPYPEQIERFDGGQ"
BASE COUNT         9519 a         6041 c         3938 g        12664 t
ORIGIN      
        1 tcgatataat catgaatcaa tcgattttta gcctcacgcg ctaagttaat atctgtgaac
       61 ttaaaaattc ctttaacagc ttctctgaaa gattttgaat tttttttagg aatggtggta
      121 aagatatttc ttaggaagtg aacttggcat ctttgccaac ttacgttggt gaaggatttt
      181 ctaatggcag agactaatcc tttgtgcgca tcagaaataa cgagttccgt accttgtaaa
      241 ccgcgttctt ttaggtattc aaaaaatgtt gtccaggtct cttcgctttc gccactttga
      301 atcatgaagc cgataatttc acggtcgcca tctttggtta ttccaatcgc tatatgacag
      361 ctttttgaga gtactcgatt ttcttctcgt acttttatat agagtacatc ggtcattaag
      421 taaggataat ttttttctga taataaacga ttctgccact cgttaaccat aggttctagc
      481 tgttctgtta agctagaaac gaaggactta gagacggatt taccacaaag ttcttccaca
      541 atttttgata ctttacgagt tgaaacgcct gatacataca tttccaacat tgaagccatg
      601 agggcttttt cgtttcgttg ataacgttca aacactgtgg gtgaaaaatg gccatcacgt
      661 gttctgggta cttttaattc tagcgtgcct acacgtgtcg taaagctgcg ctcataatag
      721 ccatttcgtt gactttgtcg gttttctgtt cgttcatatt cttttgcttg aatatattct
      781 gttcgttgat tttccattag ttgattaaat accgttgtta aaatattttt agaaacgtca
      841 tcctttacag aatattcaat aatgctttga atctcttcgc ttttcagtgt aaaatgtact
      901 tgggtcatgt aaaagtcctc ctgggtatgt ttttgtcgtt aaaaacattg taccgtaaaa
      961 ggactgttat atggcctttt tacttttaca caattatacg gactttatca aaataagcgg
     1021 aaaaacaaat gatttccgcc actaagaatt aaaattcttt ctttataact ttgtttttat
     1081 tatcaaagac aagataagca ctatattttt tattgtcttt acttataaaa cccgttactt
     1141 tctctttagt tttcccattt tctaacaatt ctttgactgt ttttgaactt aaagttttcc
     1201 cactattatt tttatagaaa ataaaacttt cacctgttgc taattctttt atttcaaata
     1261 cttttgcttt ttcaactatt tcataaccat ttactgtatc gtgtttgctc tttttatcgc
     1321 cttttggact gttcttttca aactccatta ccactttcat cacatttgga tcgaaaagta
     1381 atcttgcatt aaaagctttt ccttcttttg acttaaagcc ggaaaggact ttatctgtac
     1441 gattatttag taatagttct tgaagaactt ttttattgat tgcttttcct gatatatttt
     1501 taaatatcac gaactgttca ccactagtaa tatttaaaat agaataaact tttttctttt
     1561 cttccacttg ataactacct atatcaaatt tattttgatt tacagtcttt tctaaataat
     1621 ccgaagtaaa ttctactttt ttcaaatctg aaacagtctt attgatatag ttatagatat
     1681 ttcttaaaaa agcttcctgt gtatagctac cttgcgcaat tttcccaagt gattcttccc
     1741 atatcgctgt attttctggt tttccaatca ataggtcttt attctttgtc aaatcccata
     1801 ataaatatcc ctttggagta ggataaacat tcgtgttttc tatcttaata tattccctat
     1861 ttagaagagt gtctacaata ttagaacgtg tagcgggcgt tcctagccct aatttcgcca
     1921 taataccggt tttacctaat aatttacctt cagtcaacct tttaggttcc ttagttaatt
     1981 tatcaataaa tctaatatct atatttattg tctcttgttc atgaaattta ggtaatgttt
     2041 cctctttatc atcagattca tctaaattag tatctacttg cttccaacct aaagatatag
     2101 gtacattacc acttgtggta aattctaagc cgtttacatc taatgtaact tttgtatttt
     2161 cgtacttgta aggttctgca aacattagac acgcacgcct agtaacgatc tcataaatat
     2221 ttttttcatc taatgtcaga tttgctatat caggaatatc cattgttggt attagtgcat
     2281 aatgctctaa tactttgtca ttatctacaa accgcttttt aggctctaaa caaggtgtat
     2341 caatactgat ccctaaaaca ctcttataca cgtctaaatt cgcttttaaa tactcaaatt
     2401 catttgtagt tatcaattca ctatcggttc ttggataaga taaatatcca cgatcatata
     2461 gactttggat aattttatct gtttttttag cagaatattt ccatttttta ttagcgtatt
     2521 cttgaatacc acccaagcta aacaattttt gtggttctgt ttgcttttta ctaacctcaa
     2581 ttttttttat gattccagta ctggcggaat caaggccgaa ttttttagtg tcttcttcaa
     2641 atttttctaa tttaaagtat tctcgactat ttttgaatat aacttttggc tctctgtcaa
     2701 cagtatgaca ttcaatcact ttatacttct cagacttaaa atttttaatt gataaatcat
     2761 tttcaacaac caatgtatta acaggtgtct gaactctacc tagtgatacc accccagata
     2821 agcctttttg ttgcaatttt aatgtataca attctgtata attcattccc actaaataat
     2881 ctgcaatttg tcgtgcattt gcttcttcaa agaaggaaat agtttcctct gcatttctaa
     2941 ggtttttaaa tccctttatt atttcacttt taaccaaaga attgatccac aaccgcttca
     3001 taggcttctt aaaaatagat tgcttacaca gcttaaaaat attataagca atattctctc
     3061 cttcacgatc tggatcggtg gctataataa tttgatcagc attttctaag cacgacttga
     3121 ctacagtaaa ttgctgactc tttccctttg aaacttcata cttcaactca tccatttttg
     3181 gtaaaaaagg aagattagct aaatcccatt tttctttgtt aggtagccat tcatattttt
     3241 ccggcattga taatccaact aaatgtccta taccccaagt tacaatcgta tctccaaata
     3301 cctcactttg aaattcaaac ttccctccac tttttggaat tttcttatcg gctaacgcaa
     3361 aagcgaattt ctctgcttga tctggttttt ctgctaaaat tacagttgcc ataaatactc
     3421 tctccttcac ttaaattttt caattgaacg ctctaataca cgtgttaatt ttaaattttt
     3481 aacttcacca tcattcaatt catcaacaca catcttcttc atagattcaa tggtagcaat
     3541 ataagaaaat ggtggacaac aaatcaacga accaacaact tcattaccat ctttcattga
     3601 tttagcctta tacaaatgtt gatacacata aggtattaat tccataatcc ttcacctccc
     3661 actagattaa aacaatatct cttacacgag taattgaaga attttcagac ttctgatacg
     3721 ttaatctaat tcttgaacca tcagctaaat atcttacact ttccttattt acatcaatca
     3781 atctttttgg aataggttca cagcaataat tagtattatg ccaaaaacaa aactttaatt
     3841 tttcacctga atcagtatct aataataata ttaaattccc ttttctgccc caataacgac
     3901 atacagtttc tccctctact gtatggattc ctatttctga aggaaaatca tactctgaac
     3961 gtattaccgc tatatcgcct ttcttaaaat ctgattcatt ataaatccca tcaatattca
     4021 ttaatagccc ccttttttat ttacctgata tatattaatt ctaacaaaaa aacagccaat
     4081 atcaaactat caaaaaacag agtagctatc tgtctacttg tagtcaaaaa aaacaaatta
     4141 ttcttttctt ctttctctaa tcttcaatat attttttatt ccgttttggg gctgaaattt
     4201 tcaaatactc tttatactct ttttcatact cacacaagca atcataaacc tcattcatat
     4261 tccctcctga agctagagac aaagcataca aaaaagaaat aggcaaatca gctacctcac
     4321 gctctctagt aatccaagac gatagagttc cttgtttaaa tccatataac tcacaaaaca
     4381 cctcaacaga taaacctagc ttaccgataa taaatacatt gatcgggtgt gagtaaacat
     4441 atttcttaat taccaaagaa aatcttcctc cttcaaacat aactgcatac gcagttataa
     4501 gctgtattcc gtacacgtgg attaagggaa aagacacaag taaaaatagt tctatacact
     4561 ccatacataa aagagagtac taactactaa tcattagtat acgctaaaac aggaatagga
     4621 gtgggcagaa acactagata tgttaattgg caatttagct atttaaaaat caaaaaaata
     4681 aatagaactc taacctgtat tagctcctct aattggcgca tgtttgctct tttctctttc
     4741 tttcagtatg ttcgcatgga acggtactgt tttgtcttca taacgtctta aaatcgtttc
     4801 tacgtggcta tttgctgttc ctaataattc taagacaaat acttgtttat cttttttatt
     4861 ctgaataact gtatttaata ttttagcttc cacaaatttg cttgttgcta ttttagtcga
     4921 tccataccga ccacgagacg ttcgtaatat cactgtattc gccgttctaa gctcttttaa
     4981 aatcaccttt aacgtagata caggaatttg tgtttcttca gatatagttc ccatagagac
     5041 acttaaaaac aactcatttt ttttcaatga tctttgtttt ctttctaagt aagctaataa
     5101 atcagcttta ctttcttcaa aatgactata ttttcgttct tctctaggtt tagcaaattt
     5161 cacccaatta gacggatcga cacgattccc tttacgttta acagcttgtc tatttacatt
     5221 tattgagtat ttttcaaact cggtaggtgt agcataattt tcgataattt cattgacata
     5281 tgaactttta gcacctttgt aatcgccacg ataagcactt cttattgttc tctcaacttc
     5341 agaataggac aaatcattat tgaattgaag ttgatccaat tgatccatac actcgccaat
     5401 agataagcca cttgatttac atgccagggc taaggtaaaa atagttttat ttctatctcc
     5461 ttcagcaata tccgtatgaa ttaaaaagtc gtaccattta gtatcaattt gtcgtttaga
     5521 ccctttcttg ccagtagaat taggagctaa taatgacaag ttagacctct ttaaagcata
     5581 ttctttttct tcaaagtctt ttgaccattt caaataccct tcaaacgtat ctacgaaatt
     5641 aggttcataa tgtacaatat tttttttagt tggaaatcta aaaataccaa aatgattaca
     5701 gcccatatcg acttgtggta aacgattttg gatagatttt ctgatatttt ctgatattct
     5761 tttagcaact ctcaaagatt taaaatcatt tgaattagaa atatagcttg ccttatcaaa
     5821 tgaatcagtt tcttggttat agttttgaat cagaaaatag caatgataac cttttggcgt
     5881 atctacaatc agtgtaggca ataacccttc ttccaataga taaagaacta gtaaattcaa
     5941 atccgttttc ttttctccta ctggatagtc aatatctgct acaaaagtat taatctgaat
     6001 taagttagct tctttgtgac caaaaattgt tttgttttca ccgccaccaa gccatgaata
     6061 cacgttagga gtccagtgtg ttaatacatc tgaatcttcc aagacacgat caatagaacg
     6121 atatataaga cctttagtct ttatcatgtt tgttttagaa gtaaatccgg caatagcacc
     6181 aatcttattg ctattctttt taaaagcaac tagatttttg ctattaaact gtttaattcc
     6241 atttttaaga attttagcta aaacgtccgc atactcgcaa ctattcaaca ttgcactttc
     6301 tccttgctat caattttgaa tagcaaaaaa gacatagggt aactttagat tccgtaataa
     6361 ggagtcctaa aattacacta tgtctttaga gtaagcactt aaatgctaaa ctttaaacgt
     6421 gcaaaaatta aaatgagttg gtagctcatc ttggtggact tcgtggattg ccaaatcgct
     6481 tttcttacta cagaagttcc attaaataag attggtagtc ttatttaatt atctttttcg
     6541 ctattttttt cgttacattc atacatagta gagagtctgt aactaacttt tctactagaa
     6601 ttttttagtg ttcctttggc ttcagaacta ataccaacat tataagctaa tagaattact
     6661 tttaacaaga gtttttttaa aagattttta ccccatcact tttacttgat ttataacata
     6721 agtattttta tcaataattt catgaatgca atatctgatt tttaaagtat ttattttttt
     6781 cagtgttgtt tcgtctttgg gaaaaggcag tttccaaatt agaatacatc ctgattcagt
     6841 attaaaaatt atttcagttt ctgaaatata gtacttactt ttaacaagtt taacattacc
     6901 tatttttacg atctcatcta tttgatctcc tacgttatac atttttgttt ctcctttctt
     6961 gatatatata ttatatcacc ttgactactt gtagtcaact tggaataaaa gaaatagtct
     7021 acttgtagtc atgttttcaa ttggaaaaaa atttatttaa agattctttt aaggaaacgc
     7081 ttaaacaata tttcttagct tttttacttg caaattcagg aaagtgactg tataatcatt
     7141 tatataaatt atgaaataaa gacttcgtgg attgccaaag atttttctta ctacgaacag
     7201 tcttacgagt caacgattgt tggtagcttt cgttggctct ttatttttgc cacaaaagac
     7261 tacttgtagt cttttgaaat ttcattttca ggctacttgt agtcaaaaaa aaaggaaaga
     7321 cattttcatt gtctttcctt ttttttacga tctttcgtta gtcttctgta cggcccaatc
     7381 gttcttgtac tttcttttga aggcgttctt ctttcagata atgtttttct gtacactatg
     7441 ttagtgaact tcagcaaatt cattttctgc cccctgttca ctaagctgtt ttggattcca
     7501 aactagttca taatctccaa agaaatcttt caaaaccata ggttcattat taaatattgc
     7561 tcctgtgtcg tacaggctct cctgaagagt tttgttataa ctaccagtta aaaatacttc
     7621 tttacttcta tagatatatt tatcccactt actatccatt ttaaataaaa acagctcaac
     7681 attttttaaa tttccataag gtgtttttaa acaatagctt tccttttgaa tatcagaaaa
     7741 gacttgcttt acaatatgtt taaacagcat tttttcagct atattactaa ctaaatatag
     7801 gagtaaaaat gcaccaagtt tagttgtcgt attataataa ttttgaaaat gaatttcatg
     7861 ctcatacagg ctaagtagaa ataaaactgt gccctgtcta caaatgattt ttattccttt
     7921 ttgaaaaggc ttcaaataat taatataatt gtcatttgtt gacatcatta tttttcctac
     7981 ttgtaagata aataaagcaa aaataaacag gctgaaagca agacttccta aaatatttaa
     8041 ggcttctttc acctctaatg attcttttaa tcgatcagtt ccggcgaaca gtttataaaa
     8101 gctataagca ttgctccatc ctactctagc aatgaatagc cagaccagat aacctaaaca
     8161 actaaaacta attttcagcc ctctcactat atctaattga aaaatgagtc taaattccgg
     8221 ctctctatac atacttttaa atttaacaat ttcttttttc aatccgagcg ataatgactc
     8281 tttgcctatt tttttactca ttttactccc cttaaaccgc ttcatcagcg atttctttct
     8341 cataaagtga ataaaaaaca gacaatttaa ttggctctaa atattctaaa gttgcttcag
     8401 ggattctttt actgtcttta accgcaatat aagctgaatc attaaaataa aaaaattttt
     8461 ctacattact ttttgcacta aaaccatcat aaaaaattcc aaaaagttct ttactacgtt
     8521 tttttgaaaa tggattcacc aattcaaaaa cacgctttga aattttttct agcaattctt
     8581 tatttgttct atagaattta ctatttttat tgattccttt taaatcttct cgaccactaa
     8641 tactattttt gaatgtagtt actttatttc tactataaaa ttctccatat attgatactt
     8701 ctaaagtacc atcttgccat accaccaaat gatctatatc tataggatca cttaatttac
     8761 tgattaaatc agcaacacca ttttctaaaa tgtaatctaa attattattt aatactctcg
     8821 acttaacgta ctctgatacg ttagaatttt ttagtgcttt ataaatagct gtcattcttt
     8881 attctccttc gtagcctttt gatctacgaa tttaactacc ctttttctat atacaaccct
     8941 cctcattcaa aattagatag tcaagacgct taacaagact acatttctat tttgtatgat
     9001 ttaccgctat aattcaacct caattaaaat ttatttcgtg cctaacgatt taaatattca
     9061 gttactttct ttattaaaaa cagcaaacta taaatgataa taaaatcaaa cactattaaa
     9121 atcatacttg atattaaagc tttatttgtt aatgttgtaa tcccaatagt aattactatt
     9181 aagtttgtga ttataattct accaatatta ctattttcat tcttatgaat tagtaaatca
     9241 tagacatcaa atacgataat acaaactatt aagaacaata taaatattac cttattgtta
     9301 tatacaaatt cagttgattt tcctattaag tataaaacta aataaattag ctcccacata
     9361 ttctcactct ttaattcgaa ttttcatata cgcaattctc ctgtgattct tcgtagacct
     9421 gtttaataga agctgattta cctaatccca ttgcacctaa aatatactta ttattggcgc
     9481 atgttttttg gtattccact gcttcacatt cattctccat tttttttact gaatcaaatc
     9541 tttcgtgctt attatccaac tccatgatac gcattatctc ttgtgcattt gctttactat
     9601 taaaaatcat aaatggatat gtttctccgt ctacataaat tccccaatcg cagacaactt
     9661 ctttgatctc taatttcata aaaaacacct tcctgtagat aatcaattat cactcatgct
     9721 tatcaattga ttcttgaatg gtattgcaag gaaatgtatt gaatctgttt tcgtcatatt
     9781 ctaacgttgt atactgtggc gtaagagaac tattatcttc aacacttatt gaatcgaata
     9841 tgcaacctaa aattctgacc agtcataatt tttttcttct gtactttctg ctgtaggatt
     9901 tccaaaaaat ttcagtcgaa attctttttc aaaatcatta acccaattaa cttgttgatt
     9961 cactctcgca tagcctcttt cttttctatt tctgtcgtta cttgactgcc acaaaattaa
    10021 ttcttttgtt tagaagcttc tttgtataat tgtacacctt aaaagtttga aacaggctta
    10081 gaatccttct ctcgtaacat tgatttctca aagaacttaa aatcaatcag attcacttat
    10141 tcttttcttt attctataga ctaaatttct atttgttcct gttttttctg caattcgctt
    10201 aatcggaaca ccttctccaa gcattcttac tatttcatta tagataaacc tcttttgtgg
    10261 attctttgca gtagaagaat attcgtaggg ttttccttta tagactcctt tcttcttcgc
    10321 aattgctatt ccttgttttt gttttctctt aatttcctcc ctctcatttt gagccgtcca
    10381 agaaaaaatg ttaattacta agcttctaac aagtttttct aagtttggat caccaaattt
    10441 tgtatctaaa attggtaaat tcaaaaccat taagcccata ccatttttat ctataaaaga
    10501 tactgtttct agtatttcat cagaatttct tcctaagcgt tcaagcgact caaaaacaag
    10561 cgtatcccct tctctgacaa atgacaacat tttattgaac tctggccgtt ctactgtgtt
    10621 acgtccagat tttttctcag caaatacttt ttcgacacca atttttctaa attcttcaag
    10681 ctgtctttcc aaattttgat catctgaact gactcgtgca tagccaacta acatatttaa
    10741 cacctacaaa taattttact ctagtttaat taactaggaa ttttttcgtg agttcagcag
    10801 actctctata ccatcaaata aacattcata gtattttatt ttttctttta gctttgaatt
    10861 ttcttctttg agttcattta tcaatttaac ttgggatttt tcagaaataa agccttcatc
    10921 aaaaagcaaa tcttctacac tacaattcgc aatgtctgca atttttttta gtttggcttt
    10981 gttcggtaag ttaccaccat gttcccatgt tgacacagtg cttctaggag aattagaaac
    11041 ctcgtaccca aattcctgca tagtaagtcc taattttttt ctaatagaac gaatttttct
    11101 ccctaccttt ttagggtctg gtttaacttc tatgtctcga taatcttcaa aatcttttga
    11161 gtaatatttt gtaaatatat tttttattat aggattcaat aactcatagt ctttatttag
    11221 cgaaaacgta ttactatatc tttcttgaat gtctttaaag actttgtgag gtatttctgg
    11281 aaagtcctta atatatagct cataccctat atcgataagg tatgaagtta tatattcttc
    11341 taaagttccc catttaatcc agtcaattgt tgaattagtg atagttgata ataatttcag
    11401 cttttgtgga tttggtaaat tggtcccacg ttcccagttg gatatggtcg cacgtggtga
    11461 attatctatt ttccttccaa gttcttccat agtaaaacca catgaaaggc gtaattgttt
    11521 gattcgtttt cctgcttctg aaggatttgg cttcatacaa tcacctctct tatggaattt
    11581 gggttacttc aaattctttt gagtaatatt ttgtaaatat attttttatt atagaatcca
    11641 ataattcata gtctttatct aacgaaaacg tatcactata tttttcttga atgtctttaa
    11701 agactttgtg aggtatctct ggaaagtctt taatatatag ctcatatcct ataccgataa
    11761 ggtatgaagt tatatactct tctaaagttc cccatttaat ccagtcagtg gtggtttctc
    11821 ctactatagc aatcagctgt aactttttct gagaaggtat atttctacca ctttcccaag
    11881 tagcaattgt acttttaggt gagttgctta aatgagaacc aaattgctcc atagtcatac
    11941 ctaatttttt tctgatatat ttgattcttt tacttacgtc ttttagattg ggaatcattt
    12001 tttcagctcc ttgatttact acttgttgga tttcatacaa tctttttcgt aagctttcta
    12061 cctcatattt taattcctca ttttcttgtt taagaaggat attttcggct gtcaaacttt
    12121 catacggctc tactatatta tcagtagaag aatcgttgat taatgctact atcggaacgt
    12181 ccaatgctct tgcaatagct tccagtgttt ttatactggg attacgcctg ttgttttcta
    12241 aatcccctat atatgatcgt gatagaccca ttaactcagc aaaatcagtt tgtgtcatat
    12301 ttttactttt tctaatattt ttgatgttgt ttccaatatt aaggtttttg agttcaacat
    12361 ttgtttcgtc taatcccatc aaataactta caggaacatg gaaataatca gcaaggatat
    12421 tccatgtttc agtatttcta ggatttcgct caccacggta atagttcgta agagttgtat
    12481 agttaatacc agtttctgta ctaatttgtt taatggtctt tttactggaa tcaatataaa
    12541 attttattct attcaaagta acgcccccta tagtaaattt tattatagga ttcaataact
    12601 catagtcttt atctagtgaa aacgtatcac tatatttttc ttgaatgtct ttaaagactt
    12661 tgtaaggtat ctctggaaag tctttaatat atagctcata acctgaatct ataaggtatg
    12721 aagctatgta ttcttccaaa gttccccatt taatccagtc tacactagtg tttccaatat
    12781 ttgcaattgt taaaagccta tttttattgg gtaaagacac gcctctaagc caattattaa
    12841 cagaacttat tggtgtgtcg ccaattaaat caccgaatcg ttctaatgtc agacctttgg
    12901 attcgataat ttttctaata cgttcactag ttaacgatag attaggtttc atagctaagc
    12961 ctcctcgtga gtaaagtaat ctttcaatat tttttgtgaa tcaacccatt ttatcgagaa
    13021 gtgatagttg acaaatagca ttgagagata tttctcgctt atctggatca tcaatttttg
    13081 aaatcacatt aagataaccg gcgaagcttt caattattct attgacttcc tcaattgaca
    13141 gacgttccaa agcatccaat gctacttcag atagaaaata cattgattct gctacgcttg
    13201 aatcgacacc cgtcataaga taaagttcag aaactccgcc aatttctgca atctttttaa
    13261 ttctttctga gttaggaagg tttttaccat tttcccagtt gcttataaca gctttatcag
    13321 ctgtaggaga gaatagtttg ccgaattctt ctaatgtaat attctctttt tttcttattt
    13381 tccttatacg ttgtccaatc attttaatat ttttgttgtc atcagtcagt cctaaatctg
    13441 gtttgacttc tatatttcga taatcttcat caggagaata catgtacttc acctcctgaa
    13501 aaatattttg tgtattgatt ttttcggcag ctgctaatat ctttgtatct tcttcgtatg
    13561 tgattttttc aatcgtaagt tcatctagaa gagcttcacg gtagttgttc agtgtagtct
    13621 ctccgtactc tttaagaatt ttttcctcta tattttgtgt caagcgataa acataatcgg
    13681 aaaaatctcc atactttatc cattcaacag aagtaccacc taatagtgca attttttcaa
    13741 gacgctttct attaggtaag ttatttccct tttcccaatt gttaattgtg ctaggactat
    13801 tggcatcaac cagtttacct aagtctgcca tagtatatcc atgtttttct cgtatttgct
    13861 ttattcgatt accagcctca aaaagattta tttccattcg acacctccta atgtatatca
    13921 tataccgaaa tcatataaat ctatttcaat cagatttaca aaacggtcat tcataaaaac
    13981 tagggatttt ttcgtgagtt cagcagactc tctataccat caaataaaca ttcatagtat
    14041 tttatcttct cttttaactt tgaattttct tctttgagtc catctatcaa ttcaaccgtt
    14101 tgagtaatat tttgtaaata tattttttat tatagaatcc aataattcat agtctttatc
    14161 taacgaaaac gtatcactat atttttcttg aatgtcttta aagactttgt gaggtatctc
    14221 tggaaagtct ttaatatata gctcatatcc tataccgata aggtatgaag ttatatattc
    14281 ttctaaagtt ccccatttaa tccagtcgat agttttgtta cttaattcag caattttaat
    14341 taatctttct ttgttgggaa gggagaatcc tcgttcccaa ttattaatat ttcctttttg
    14401 agcatttcca attaaatctg caaattgttc catagtcatt ccaaaattag aacgaattga
    14461 tttaattctt ttacctgttt caacaggatt tggtttcata caatcacctc ttttacagaa
    14521 tttggtttca tagtatctta ttcgctcttt gagttctaag ttctctttct ccaatttttc
    14581 tatataatat tcttgatcct gtttcttgtt caaaaaatga cctccaaatt tcacattgtg
    14641 ttacacccta gtgttacacc tccaaacctt attatactgt actttaccat atgatacaat
    14701 agggtatacc ctattgtgac acttacattc tacttttaat gtaaaaactc ttgaccttta
    14761 tacgcataat gcgtataata atatttgtaa ggaggtagtc gcgaatgcca cttaccggta
    14821 aaaaaatgct caaactagcc aagaaaaatg gttggaaagt ggtgaggata actggcagcc
    14881 atcatattgt agccaaagaa ggattcccac ccgttcctat tcctgttcat agtaatcagg
    14941 atttaaaaaa agggacagag cagaagttac taaaggactt agggctgaaa tagcccagtc
    15001 ctttagttca ttggattaac tcgaaaggag tggtggaaat gaaaaaaagt taccctgtaa
    15061 ttttttctgc tgaagaaaat ggctactcag tagtttttcc tgattttgct ggagcaacac
    15121 aaggagataa ttttgaagaa gctatgtcca atgctaaaga atttcttgat gggattctag
    15181 cttactatat tgatgaagat atttcagttc cttctccaac agatattcat caactaaata
    15241 atttgggcaa taacaaaatt gtttctctta ttcaaagtga tccttctcca tttatccaaa
    15301 aaaagaccgt acgcaaaaac gtcacagtcc cagaatggtt agtagctaga ggggaaaaag
    15361 ataaaataaa tttttctaaa gttttgacag atgcgttact aaaaatatac gagtaaacgt
    15421 aaatatatta ataacaattt agttgcctaa tgacatagat agcggaatta aaagtaaaat
    15481 agtactcatt tattgcaggt tcccttagaa tcaatacaat aaatgagtac tatttagact
    15541 gtttaactac tagtaagata tagcgatcat cagtttcagg gttatccctt ggtgaccata
    15601 gttttgcgta tttttcaact cgttgccaaa tcaacgattc tattttttta tttctactta
    15661 actccaaaac ggagtacctt ctttttcttc gttagctcct atttaagagc catgtcgttt
    15721 tatataactc taaaacagaa actactgatc aattcaataa agccttattt gagaactgtg
    15781 ttgttttaca tggttttaaa acctgatttc ccgcaaacgg tttatccgta acatttgaga
    15841 actgtgttgt tttacatggt tctaaaacag attagggaag atcaatctat caatctatat
    15901 ttgagaactg tgttgtttta catggttcta aaacctcaac aatattcgtc cttccgtctg
    15961 gttacacaac ttgacagagc taacccttca gtgagtgagt gtcccctcac tatttcgttg
    16021 ccaaatcaac gatttcttat tctactcact tttaacttct attccatatc ttttcccttt
    16081 atttattatt tgttctttca actgataata agcccattcc ccataatttt tatctggttc
    16141 agaaatacag attatttgcg tcttattgcg gatagctgtt tgaataattt ttgccgaaca
    16201 ttgattatta taggttttaa caaaattgct ttctcttttc tttatggctt cccatctatc
    16261 ttgatctaat tttctttttc gaccatgacc gcctttagaa aaaactcctc ggctttgctc
    16321 ccttgatttc attgctttaa ttttattttt gaagttactt atatatttgc tgtcaccaaa
    16381 atcaatcaca gattgattgt taactaaaca tttaccgcta cacaaatcag taaacattat
    16441 ggttaattca gctccttctt tgaaatctag cttatgataa tctttttcaa aagaaattgt
    16501 taaattcaaa aataaatttt tcccctcaaa gattagatta ctctggttta acttgtattg
    16561 accgtccata atgcgttcta aagttaaagc gctatctctg gcttttcttg gttttctacc
    16621 taaatttact ttaaacctaa atcctttagg taaagagata taaaacttgt cagctttgtg
    16681 aatcttaata tcccgacctc tgaacggtaa caatccatat ttatttttat ttctatagat
    16741 tctaggtgtt ctttgcccag taacaattcc aacaaaatca ttgctaaaat ccttactagc
    16801 tttcatttta gctgaatcta taaaatccga ataatcaaag tccaattttt ttgctatatc
    16861 acgtattctg gtttgacttt ttaaacctat agcttgtgtc aaaacgtctc tggcttcttt
    16921 gtttttcgca attcgtagtt tagataattc ccttttcgtt ttatctaatt tcttttgatc
    16981 gatcacttca ttacttagta aattatcaat tcgctcttca agacttagct ctcttttaat
    17041 atactcatga tccaattctt ttatagaatt ttcgatcaat tcttcaaaca tcagctgttt
    17101 atataccata ttcagcgctt ttacatattc tctactattt ttccaaaaaa aaccaaaaaa
    17161 cgaatctctt tcctcttgtg atccatcaac atatacttct atttttttag tcatagttat
    17221 tattgtcaat tttagcacct cattattata tatctggtat ataataatag taacatattt
    17281 cctagactat aatcaatgat caattgtcta cttgtagtaa ttatattaaa aacaaaaact
    17341 aaaaataata tattattcct tctgactaca tatagtcact ttatccaaaa aataagaagt
    17401 gctttcgcac tccttattaa atttatttac tattaattga ttcagctaca acttctttta
    17461 ttcaacttca aaatcagttt tttggatatt tctatctcta tttaattcca tacttaatag
    17521 ctcactatca gtttttacta aaagctcacc tacactatta atatatgcca atttacaaga
    17581 atttcttctt ttatcttgac ctactaattc atcttgagat acccctgtag aaaaactaat
    17641 tgatagaatc cgttccttga tttcattttt atggttccta taaaaatcat cgacttgcgc
    17701 ttcttttgaa aaccgatcac taataaaatc atcacttgct gaatatattc tttcaagggt
    17761 ttcttttgga ttcatctctt cttgagatat atccatcata atttgtttgc ttataggagt
    17821 ttcagcattt ttctcttgaa tagctttggg aacccaagca tattcttgcg tattattggc
    17881 tacaactgta tgattttctt taacataaat ataagaaaac tcatctgaat tatcatagat
    17941 acttacatta tcaaaatatt tctctaactc tgctaagttt cttaaagaaa attcgtatct
    18001 ttttcttatt aaatcttcag gtacaccgtg gccaccttta gcaacacggc ttttaaccct
    18061 ttcaatcgct ctttctggtg aatctagtcc tatatagatt aaaaacgatt cataacctaa
    18121 ttttttagca tttagaagcc tctttttatc gggatatgga ttaccgccaa aagtcgtttc
    18181 atggtgaaaa gattttcctt ctttaaaatg accttcgatc tctctcagaa cttcacgcat
    18241 acttttgttt atatctgatt gtttattcca atttcctcca aaacgcctca aaatttcatc
    18301 tgcattaatt ctatcagtat caagttcttt tctttttgaa ttttgaattt gataaatcgt
    18361 agatttaccg gcgccattaa cgcccccaat aactacataa tatggtttat cattttctaa
    18421 aatcatattt taaaatcttt ctgattaatt actttttttt gatctactcg tgtcacataa
    18481 ctagagagta caaggtagaa agttctctct tcctcacttt ctgcattttt taaggcttcg
    18541 tgaaaatcat ctacagtcat attcaaaaca tcaggataca ttttctttaa ttcttttaca
    18601 gcctcggtca tatctctcat tcccttctta ttagtcttta ttaaagttct ctgcttcagc
    18661 ttctagtcta tctttcaagt cttttaaaac aaccgtaagt acatggtctt caccattttt
    18721 ttcaattgat tgttttaccg cttcatctaa cttagctaaa tagtcttcta taattcctaa
    18781 attgtttact tcaataagtc ggaagtccgg taaatttttc atccgaatac cacctttagg
    18841 ctgttccttt acaaataaag atttgtaatc attatattca ttcccattaa taaccaattc
    18901 tttcataggc tctaagtgga taaatttacg tgaagcatag tctttcataa taatctctcc
    18961 cagtagaaaa tcttcttttt tctattatat cacgttctca aagcgttgag ttattggatt
    19021 gtttaaacca tcaagacaca tcatgctgca ttttttcatc actaccggtg cttttccgtt
    19081 tgctttaact accgctacat tagctaacaa actaattact tctccctcga taagctctcc
    19141 gctcccaata acaacgccct gtacttccat acctacttta aacgtttctt ttttatttac
    19201 cattattccg ctctccttcc acgactactt gtagtcattt ggatattttt taggggaacg
    19261 gttttccgtt ccctttcatc aaatagaatc cttcatcatt tcaacaaata attaactttc
    19321 agtgtaaaaa aattcttcgt aattaccatc atgaaaaata tcccatcttt gttcgtcatt
    19381 ctcactgaag taggtaaaat aatatctgac agtatctaac ttatttaatt gctctctggt
    19441 tttgtaaata caatccaagt gatcttgcat tcgatctgac caaaacattc ttgtctgctc
    19501 catctattat ctctcctttt tattaaatca agggtttgag tctttgacta cttgtagtct
    19561 atggctatat catacaggaa tgactacttg tagtcaatag ttttctttag tttaattaac
    19621 tgtctattcg tagccaaata atgtaaaaaa acgcctagtt tacactagac gtttaaacaa
    19681 ttcaataatt cttcgttata ccccattaag ggttgaactg ctttaatact tacttgatcg
    19741 tcttctctaa cggcttctat gcttacccct tttgtatatt ggccatcttt cttatccgtc
    19801 caaatttgat aataagtaag cccatatcgt tcaataatct caaagagaat agttattaat
    19861 tttttttgtt taaattttgg taattcaata cgcaatgatt tttcttgaat atcgaactct
    19921 ggatttttat ttttaagttg ttcttcaata atcatatagt ctgtagacgt cttatctaaa
    19981 tcaattaatg aatctgataa gattgctatt gtatcgttca taacacatca actactttct
    20041 tcttgttatt ccatttcata tacaggagta ttaagagata atttatgtgt gaagttcacg
    20101 atttttgata catcttctaa cttgaagcgt ttaaaagctt cttttcctct tgttaaatta
    20161 tcattccaat caaatccctc tcgcaaaggt acatcgactt ttacttcact gattcctatc
    20221 ccttctcttc caattccttt ttctttaaat tcagtttcaa taaaagtacg tccggcttta
    20281 tcattatcaa cacaaaggac tgtttctgaa ggcaatgatt taaacttatt ataatgctct
    20341 gtaaccccat gcaatacatt acctaccttt aatccattca tgctaacaaa cgtagcattt
    20401 tcggcttttt ctggcttttg tgtaatgtac gacatcaaat ctatttcact ttcaaaaaaa
    20461 tacattttat ctggttgacc aaaagttacg ttaaatcctg aatactcttt agagttttct
    20521 tgtatttttt tccatgtaac atccttttgt ttgccttcat gaaaaaattt tctagttcct
    20581 tgttccgtac acccaacgat ttcttgatct tttttccata agaacaaaac atttcctttt
    20641 ttatcttgtt taattagtcc tttagataaa aaatcttcca ctaaagctgt atcgattctt
    20701 ctctcattta ctaaataaga ctttgcaaca gataccggca cttcattttc tttctgatat
    20761 tggtacggag cttttttcac ttttgtattg aacgaatgaa cttcataatc gctatcttca
    20821 agtttctgtt ttgcttcttt aaatgaacaa tcatagtagg ctttgataaa atcaaaaata
    20881 tctcccccta aatcctttga gttccattta aaaaggtttt tgcgatcact gattacacaa
    20941 gaatcatgat cttctaatct atagtattcc cttgaatctt gctttaaacc tatcccattt
    21001 tgtttgcaaa aatcaacgat acttactttt tttgcctttt tcaaatccaa acaaaaaccc
    21061 cctttactag accatcacta gtaaaattac gataaggtac acttgtattt ttcataataa
    21121 tcctctgtac agaaaataaa gtttagccca tccctaagag ataactccca aaagcctatc
    21181 ttcttgctct ttttttctct aattatcttt tttctaatac tttttccgta cttcaaagcc
    21241 attttaaaag cgatttgtac atcttcacaa attacaaaat gactattatt aatttgacga
    21301 tattcacctt gcgtttctaa aaaatttcta ttttcttgtt ctatcattca tttatctact
    21361 ccatttcaaa ttttgttgta ttttcaaaat tagtcaattt caaaatctta ttctccattt
    21421 cttcagaaac aggtggtatt tcaaacgtat cattttcaat ttcattataa atttctctca
    21481 ttgcaccgtt gtaagccatg atagacgtag cttttttgta ttccgtagaa gaagtatcta
    21541 ctggaaaacc tagcagttct tctttatcga tctctaactt ttcggataaa tttgtcaatt
    21601 tcgtttcaat ttcttcttta tgactatcat aaaattcatt aatatcacta tctaatgcta
    21661 atctggaaac tggttcttcc atgttttcta agtattcaat gcgctcttgc caagcttgtt
    21721 tgtcataata attagttgtt tctagctctt gctcaaaatc ttttatcaca acttctcgaa
    21781 tgggaacttc cgaaacctta tgcccaacaa atttatctgt catgtcctca ctactttcta
    21841 agctattccg ttcctcgttt aaatcgtgaa tagtttcaat ctttacttta tcaggctgta
    21901 acactacgat aactacatcc ttttgtctta actgttcttc cgcaataata tgtgtatatt
    21961 tcccaacgtc ataaaatgcc ttagcagtag cttcatgatc tccaattgta tttccaatgt
    22021 cgttaatcaa atctacgtca ttttcattct cactaagaga agcaatcact tctcttttta
    22081 caactttttc tccgtaataa ctaacatcat tgtaatcatt taaaatatca ttactttctt
    22141 gtaatttatc tattacttta gaaagctctt catcagtaat agtgcgttca ttcatagaaa
    22201 tcggcttctc taattcaaca tttacttcat atacgaaacc attatttccg ttactaccct
    22261 gttcagcgta cattgtagcc atttctttat taggtgttaa gtacacacca aatccattag
    22321 ctgttccgtt taaaccttta tgatctaaac taaattcatc aaatttattc atacttccat
    22381 gaaacaggcg ataagttccg cctgtttcat aattttgttc attactcata attttttcac
    22441 tccatttcaa aagaacgttc tgctgttttt acagtcggct tttccaagtt agaattttct
    22501 ggtatcttga aagttccttc atccatttca tattttactt tagttgcaac caatttataa
    22561 gctaccactg aagccacctc tttatatgac tcattgtcta ttgtatctct taaatttgtt
    22621 cctgacacag attcaaaatc tgtaatcgta ttttcgattt cttgtttatg agattcgtaa
    22681 aaatcttccg cagtcataca agagtttaat tcttttacat cattaatatt tgtttggttt
    22741 tcaatttctt cttgccaaca tttttcatta tagtaattgt tagcattttc agatagatta
    22801 taaaccgctt catttataac aactgatctt agcggttcgc ttaattttaa cgtatcattt
    22861 tttagttcat gattctcgca agctttcaaa ctttctacaa caaacttttc aacataatca
    22921 caattttcag gtgatcgatc taataataaa cgatcatttt tattttcact tggattctca
    22981 aaaactacac cataaacatc acatacatca aattgtttcg tatctgaata atatgtcaca
    23041 tagttttctc ctattttagc tgtaacatca taattgctat gagtaagctc aatagaattt
    23101 tctccatact ttcctctata ataatccacc atcgcttcta aataactacc gtggatcgtt
    23161 ctatccggat cgttgattgt actatcgtag aaattatgaa attctaaatc tgatttttgt
    23221 tgtatctgct ctttattctc attaggaaca aaggaatttt ttagtgcatt aaatgctgtt
    23281 tgctgttctt ccaagacttt cttatcaatc gtacttataa agtcaataga cgtttctcta
    23341 acttcttgca ttatctgtct aaagtttttt ggatcaattt tttcattatt tttagtccaa
    23401 tttgcaatat aaggaatagt aaagtctgac gtatcaatac cgtaattgtg acaaacaata
    23461 aatgaagtca tttccgcttg aaactcttta attggtgttg agtaggaaga accgtgttca
    23521 ttcttattgt gaagcttttt gtgagccaat tcgtgaatac tggtagtaag attttgtgtt
    23581 ctgctatttc ttgaatttaa tacaatttct tccctaccat caacatattg aatatattca
    23641 cctctagcgc ttcccatttc acctctacca gactcgttca tatcttttat agatatattc
    23701 atagattcgg ccaaatgatt tacacctttt tctaattgac taacacttaa ttcaccatca
    23761 atattaaaat tccataccct gttaggaaat atttttggca aatcagcttc agttgcatta
    23821 gtttgggaaa tatcaaatac gtaacccttc ttaaaatgct tgatcttttt gcttcttaat
    23881 tctccatttt tgattttttc tttttgctcg cttgttgctt cttttaacgt cttttcttca
    23941 ccattttcat caataaaaac cgttaccgga gcatgcgcat aaatgcctat ccctttttct
    24001 ttcgctttaa cttgaaatcc tagttttttc caaccatcat aacttgctac agcaattgct
    24061 ccttcaaatc cctcatcgat caacgatagg tttaaaggac tgtaattgtg tattttagac
    24121 atgaacttgg caaactcgat catatcttca gggttagact gatattgctc gattttgctg
    24181 ttggaagcct taattaaatc gtctagctct ttttgttttt gctctttcgt tttcggcttc
    24241 cagtttcctt ttttataggc catcttctat ctcctttctt ttttaggtaa gttaaattac
    24301 ctaattaaat tataccagat agataaaaac tcttctatct aatttcactg acttaatgca
    24361 aataaaaaag agataagata gttatagtaa ccatcttatc ccttcttatt ttaccatcta
    24421 ctcaactata cttacgtttt tacctttact atctaattga actttgttat ctttagaagc
    24481 tactttagta agcaaaagtt gatccaattc atcattagaa tatcctgaat aaaaattatc
    24541 taaaactttc gttgtggcaa ctggaattga taacttcaaa tccatactag tagattcata
    24601 agataattct ccttcagacg aaacttcacc aaaactattt aaattccaaa ttggactatc
    24661 taaatattta taagataatt taacggtttc cattgaaggt gatttgtttt cattatccat
    24721 tttaattgtt gctgatacca ttttccactc cggctctgaa tattgaacac catcgatctc
    24781 ttctatatcc gcctgtattt taggtatact atcttgaaaa tcagcaattt tttggtcatt
    24841 ttgaaactct tgtttttcct cattttctaa ttctgttttt acagaatcta aagtggcatt
    24901 atagctatca atatcgcttt ttttcttatc attaaacttt tgtttatcag cgttcaatgc
    24961 ttgttctatt atttcttcat cactcatttt agaaactttt tccaatgtaa gctgttccat
    25021 atccgtttta aatacttttg ctttgccatc tttagtttgg atcaccgctt ttacatattc
    25081 atttttatca ataggcgttt tatttttact acttgtttcg actatcaacc aacaacgtga
    25141 gtctttttgt tctgcactat ctatgaatga gatattatgt gcttcatttg acttagcacc
    25201 agcttcttct ttgccacatg cagataataa tagcgcaaaa ctaaaaatca taggtattgt
    25261 tgcaataact cttaactttt tcatttataa gcacctcttt tttatcggtc tgatatataa
    25321 ttttatatta tcatattttt attccaatac acagatactc ttatataatg tcttttcatg
    25381 tagtttatca aatttctatt tcaaaccgct taaaacaaat atcaaagccc aaaattttct
    25441 actggaaact tttagagcct aaaatatatt ttttatttga tccaaaactt cagggaaata
    25501 caaacctaat atcaacacag cacctataat taatagcgga atcattttct taataaaaga
    25561 gatcacagca aaaactatca gtactgcaac taatactaaa gctgttttgg gattagaata
    25621 taaaaactct ttgactgtag acgttacatt taagtatagt tcaatcaata cgcgaaaatc
    25681 tccattactt agtttatcca taaccgttct aaaatcgatc atatcatttc ctcactcttt
    25741 ccaattttat tgcttctttt attgaagaag caacttgttc attactatgc ttatctatat
    25801 ataatgggga aaagtaattt ttatcaaaaa agtaatttgc aaaagctaca tcgtcttctg
    25861 tcttccaatt ataaaaacag taattcccac taatcggtat ttcaaaaagc ccccaattct
    25921 ctagctccat gttttctttt tcaaacaaca ctatgatttt ttttacatct tctatagttt
    25981 ccgaatcact gttaagtagc tcacgtaaat atatattttt tgtattactg cacactatat
    26041 cgaagttttt cataatttct attctccgtt tctactaatt cattcctatt tcaaattcac
    26101 gtgcttgtct cattttctct tgctctatca ttcgttgcgc tacttcataa tcatttttcg
    26161 cacgatacat atttaaatca tcattgatta ctctatcaat ttttctaata agctttttat
    26221 ctttaaattt ttcagaaatc ttcttgaagt ggacttcatt tttttcacgt gctaattgtc
    26281 gttcttctaa tcttttttgg aaatcatcat aactttgttt agcatatttc gttttaaatt
    26341 ctggtttttc tctatcaaac ttaacgttta atttttttcc gtctaataga tttttaagtt
    26401 cattactttt caaatttttt tcttcagatt gctttaaaag tttgatttgt cctactacag
    26461 cattacccat tcgagcatta aaatcttttt ttctatttaa ataatattct tgtggattgt
    26521 agcgtttacc gtaggttgtt tgatatagct catcaatttc tttagtcata cgatcatatt
    26581 cttttctttt tggattatcc tttgtcatat aatttgtgac ttcatcaatc ttttcttttg
    26641 tttcttcagg aagctcattg tagccacgtg ttgtactagg tatttctttt tttaattcct
    26701 ttaggtactt tactaattct ttgtcatatc ttgaattatt ttctacatat aattgaatca
    26761 aagaatcttt tacagactgt ttaagttcta aaggaacttc ttttctcaac ttagaaattt
    26821 tttcaaactc gttcgttcta tctaatattt tgctaccgaa tttaaaaatc atatcgtcta
    26881 acgtagactg ttttctcttg cccctagctt gtaaaacacc atcatattcc attatttcac
    26941 gagtattttt acgctccata gctgttacat gaatatggat atgttcagta tttctgtgaa
    27001 tcgttgcaaa ccaaaaagga tcaactagtt cttccttttc ttgcattttt cccatcatag
    27061 actttgttgc ttcatataga acattttcat ttaattcatt tgtttctgga ttataatatc
    27121 cttcccttac taaaaaatca ttatcgaatg aaattacatc ttggaacatc accgatccat
    27181 tattttttgc ttctatgacc gattctttta tttttgaaat gtctttttta gtgatatatc
    27241 tttttgtatt gctaaatgct ccgtttacta tttcattatc tacattttct aagatagctt
    27301 ttttacgtgt catataatca atatatttat ccatttcacg taaatcttta atcactggtt
    27361 gatctgtttc ctgttctatt ttttccaaag ctttttctat tctagctaat tcatttagtt
    27421 catcttctga ataatcatga tctagtttat cttgcctaat agcctcttgt ctggaaatat
    27481 aatctacaaa tttttgaagt tctaccatgt gattattctt tttgcttttt ccagtttcaa
    27541 attttgcttt aagaataatg ctcgcacttt ttctgttaaa gatatgttca gtcatatcat
    27601 cacccaccat tttcaaaaat tttttctaca ggcggttttg tacttaatgc taagttgcat
    27661 tagaattttt tagtgttcaa tccgttgtaa atcttaatga cggtgaacta aacattgttc
    27721 cctctcccaa acgactactt tcttgttctg gttcagcacg ctcttcttct aattctttac
    27781 tagctaatcc actattaatt aatagtttat agacagactc ttctatacta tttaattgtt
    27841 cactataatc tttatgtgta ctgtcttctt ttttattaaa gttttcaagt tgctgttttt
    27901 gcaattctat tatcgtttca agttcgctaa tttttaattt tgcttcttct aagtttttag
    27961 ctaactcttc tttttctgga tcacggaaat tttgattggc ttttgttaac attggtaaat
    28021 ttgaaacaac ataacggaac atattggatt ttgtttgaaa tactgacttg tttacttcaa
    28081 agcacttatt gaatttttct tttacatctt ctgttgctga tactttgaaa taagggtagt
    28141 tcatttattt aaatcactcc tttcgctaat taaaaaattg aataagcgtt cataaactga
    28201 agttaactcg ctaatacttt ctaccatttg ttcatttgac aataaaagtt tttcaactac
    28261 taaatcaaat tctgactttt tatagtcaat caaatcgatc tcgctatctt ggcgaatgat
    28321 tgctaaaaaa tagtcattcc tactaaactt ttttccggta cgttcttgaa ttttattaac
    28381 ttttttatca atctctttta catattgtgg atcaattcct ctgattgaaa catccataca
    28441 cacacctctt tcaagctgtt tttatctatc tggtatatat tattatatca aagtccaaag
    28501 gaaaattaaa gatttaaaga caagaccaag gaattaatac aaagagcata agggaaaact
    28561 gatagcacct tcctaaagga aggtggctaa gttggctgtg ccaactggtt ttctttcaaa
    28621 atagcttcat attttttgct atcacaaaaa aatccatttt cgacctattt tcggtcataa
    28681 tatagtacct acttttggtc atagtttcgt ccgtagtttt ggtcatagta actaaagctt
    28741 ttacgcccaa aaaaagagtg gcaagtttta ccttgccact ctcttttcta ttcacaaaat
    28801 tggtttaaat tcgctttttt tagcacttcg tttatatcat agcaatcata atatttacac
    28861 aacatcattc ctaattttaa cgcttgttcc tctttatatt ttttaagctc tgcatttttt
    28921 ttgcgcttaa tcgcttttct cttttcttta tattccgctt caattctcat taattcttct
    28981 tcagctttct cttgaatagt gacaatagaa ttacacattc tctctaaact cccttaaaat
    29041 aatttaaaca cgatcactca aatcggcttg cagacttttc tagttgcttt attttttcct
    29101 caaattgttt gattttattt tcactttcct ctattgaaga tttattattt tcaatagtcg
    29161 tctgcataga actgatttca ttttttgtat ctgtttgttc gtctagggtt tgaaattcta
    29221 agttttgatt agctttatca atatcttttt gcactacttt atttgctaaa ttcatttttt
    29281 caattgattt tttctcatct tttatagaat tttctatagc tttaatctga tatttaaaat
    29341 tatctttttg caaatcagaa gtactttcaa cttccaattt agcatttgat ttaatatctt
    29401 tgtctactgc atagaatttt atttcctttt cttttaagtc atttgaaggt tctaattcag
    29461 gataaatata tctagggtta actgtcacag aaattacacc aaaatcttta ggcagatttt
    29521 tataataaat taccgtgtat tcatctgaca attttacaac ttttgtctta acatcttcat
    29581 tatttttgtt cgttataaaa tttaatttat agtcactttt aatattactt agtgatttag
    29641 tacctgtatc atcttgatac cgcaatacta atttaaacag tccggcttcc ctatttattt
    29701 ccatatcatc aacatacata ttcaaagaag acgttacatt ttgggctgaa ctcattttta
    29761 cagtttcaaa atcataactt ttccccatca cataaggaga aaaaacgatt atcattccta
    29821 ttgctaaaaa tccaagtata gcacctaaat ataattttac tcctttcgtt tttttatata
    29881 ctttgtcaat ttgtgcctta ttttttttat ttggttcctt cttattatga atggaatatt
    29941 tcttattcat atacttcacc tcactatttt aagtacttga tttcataatt atctaccaac
    30001 acttggccgt ctttattggt tacttcaaaa agcatataga ctgtattttc tgtatctcca
    30061 ctagaagatt ctgttttata atttacttca gcaaaataat taatagtatt ggcgttaaca
    30121 taattcgtat ataattttga atctttatat ttacttttga atgttggatc acttgaatat
    30181 gcttctcctt tttgagtagt agatttactt tctaaagtct ttttactcgt tgtaatcatg
    30241 tcgtccggca cgatagaatc agctacagaa ggagtagcta ttttctctaa atcagctttc
    30301 ttttcttcta ctttttcaat atcatatacc ggatatagct caacaaattt cttagcaact
    30361 tcagcaattt gtttatttaa ttcactgcca tcatcattag ttaagttcgc ttctacattt
    30421 gcatttagat cactaatatt tttacttaac ttgctatttt gttcttctag ttgtgtaact
    30481 tcttgatcca attttttatt ttttgcttga atgttacttg tatgaagttg agataacaat
    30541 ccacctaaaa ctaaaataat aattaaaaca ccacaaatat atttccattt attatcattt
    30601 tttctcttaa tcatctattt tcttcctcct attttttgac tctacgaatc tctggactat
    30661 gtgattgcca atatgaatct tgccaattgt gataaccaat tccgcttgaa ttactgtcat
    30721 acatagttgt ttcattgaca taaataccta cgtgagaaat gaaatcaggc gctccatatg
    30781 ttcctctaaa gaaaatcaga tcaccagctt ttgcttcttt aattgatact gcttcagttg
    30841 cttcatactg cgttactgta taactatcta ttgtaattcc ggcgctaaat tgataaagat
    30901 aagctgttaa tccgctacaa tcaaaacctg tcgttggatt tttaccgccc caaacataag
    30961 gccaaccttg atatttttcc atttctgttt tcatttttcc ccaaaattct gaaggattag
    31021 ccggattacc actaccccca ccgatatact gtttaactaa ctctgcatag taaaaattac
    31081 caccatttaa atacaaatat gtttttccat atttaattga tacttcattt acatatgaat
    31141 aagtctgtcc tgtagtattt cctaaacttg gagcaacaac atttttagag taggcttcag
    31201 ccgtttcgat agtatgtgtt tttttgtttt ttcctaagta gtgcatgtaa gctattccgt
    31261 aattgtaagc ttgaactatt ccatcatagt tactttccat gccataactt ttagcaatgt
    31321 tatacacatt actcaaatgt tttagccctt gatcgatact ttcttcatat tgaagtgtgt
    31381 ttacaggcaa tccggctgat tcgctacttt gcattaaatc cggcaattta cctccggatt
    31441 cacattgcat aattgctaaa gcataaggta ctaattcttt aattccgtat ttttcagcat
    31501 atttttctac cgttttttgc catttttgaa cttcaggtga caacggtttg ttgccaccaa
    31561 tgacttctgc acctcctgta ttagttgcag tttgtgttcc ttgtaatgta gctaaagaac
    31621 ctataactaa aaggccaacc aatgttatca gtcctataac tacccctatt tttattttta
    31681 aagcaagacc tattttcttt gatacttgct tcatcccttg acgagcgagt gttgaagcaa
    31741 cattattatt tttgttctgc tccattaaat tcacctccga tttctattat tgaccaccat
    31801 cgaatcgttc aatttgctct ggatatggat caaaattaat catataggtg tcttcactgg
    31861 aagtttgaac tacagcttgt cctacttcta attctggtaa aagcataatt tctgtatcag
    31921 taattgtatt tcctaaaact ttttttatgt cttccaacaa tgcagaatct aaattaaaga
    31981 agcatttaaa agtacataga ttaaaaatgt ttctaatcac atctgctact tctgatccag
    32041 aatctttagg gataatttct tcaggcgttt gagttgcaaa ataaacacct gcacgcattt
    32101 ttcgcatttc tcgctcaaaa cttagtacat attcacaagc atacttgtta tttgaattga
    32161 ta
//
LOCUS       DACEDD010000032        31309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEDD010000032 DACEDD010000000
VERSION     DACEDD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.032"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..110)
                     /locus_tag="IWO45_001655"
     CDS             complement(<1..110)
                     /locus_tag="IWO45_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0333133.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVK"
     gene            complement(140..1231)
                     /locus_tag="IWO45_001656"
     CDS             complement(140..1231)
                     /locus_tag="IWO45_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0333134.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(1332..1766)
                     /locus_tag="IWO45_001657"
     CDS             complement(1332..1766)
                     /locus_tag="IWO45_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0333135.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(2350..3807)
                     /locus_tag="IWO45_001658"
     CDS             complement(2350..3807)
                     /locus_tag="IWO45_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0333136.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(3804..5699)
                     /locus_tag="IWO45_001659"
     CDS             complement(3804..5699)
                     /locus_tag="IWO45_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0333137.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(5757..7532)
                     /locus_tag="IWO45_001660"
     CDS             complement(5757..7532)
                     /locus_tag="IWO45_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0333138.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(7582..9078)
                     /locus_tag="IWO45_001661"
     CDS             complement(7582..9078)
                     /locus_tag="IWO45_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0333139.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(9272..10894)
                     /locus_tag="IWO45_001662"
     CDS             complement(9272..10894)
                     /locus_tag="IWO45_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0333140.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(11223..12536)
                     /locus_tag="IWO45_001663"
     CDS             complement(11223..12536)
                     /locus_tag="IWO45_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0333141.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(12781..13005)
                     /locus_tag="IWO45_001664"
     CDS             complement(12781..13005)
                     /locus_tag="IWO45_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333142.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(13025..13393)
                     /locus_tag="IWO45_001665"
     CDS             complement(13025..13393)
                     /locus_tag="IWO45_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0333143.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(13589..14299)
                     /locus_tag="IWO45_001666"
     CDS             complement(13589..14299)
                     /locus_tag="IWO45_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333144.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(14317..14813)
                     /locus_tag="IWO45_001667"
                     /pseudo
     CDS             complement(14317..14813)
                     /locus_tag="IWO45_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(14850..15302)
                     /locus_tag="IWO45_001668"
     CDS             complement(14850..15302)
                     /locus_tag="IWO45_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0333145.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(15437..16042)
                     /locus_tag="IWO45_001669"
     CDS             complement(15437..16042)
                     /locus_tag="IWO45_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0333146.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(16492..17112)
                     /locus_tag="IWO45_001670"
     CDS             complement(16492..17112)
                     /locus_tag="IWO45_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0333147.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(17114..17983)
                     /locus_tag="IWO45_001671"
     CDS             complement(17114..17983)
                     /locus_tag="IWO45_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0333148.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(18006..18413)
                     /locus_tag="IWO45_001672"
     CDS             complement(18006..18413)
                     /locus_tag="IWO45_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0333149.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(18432..19721)
                     /locus_tag="IWO45_001673"
     CDS             complement(18432..19721)
                     /locus_tag="IWO45_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0333150.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(19748..20050)
                     /locus_tag="IWO45_001674"
     CDS             complement(19748..20050)
                     /locus_tag="IWO45_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0333151.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(20075..22168)
                     /locus_tag="IWO45_001675"
     CDS             complement(20075..22168)
                     /locus_tag="IWO45_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0333152.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(22657..23385)
                     /locus_tag="IWO45_001676"
     CDS             complement(22657..23385)
                     /locus_tag="IWO45_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0333153.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(23531..26152)
                     /locus_tag="IWO45_001677"
                     /pseudo
     CDS             complement(23531..26152)
                     /locus_tag="IWO45_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(26266..27597)
                     /locus_tag="IWO45_001678"
     CDS             complement(26266..27597)
                     /locus_tag="IWO45_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0333154.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(27563..29041)
                     /locus_tag="IWO45_001679"
     CDS             complement(27563..29041)
                     /locus_tag="IWO45_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0333155.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(29346..31181)
                     /gene="tdc"
                     /locus_tag="IWO45_001680"
     CDS             complement(29346..31181)
                     /gene="tdc"
                     /locus_tag="IWO45_001680"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0333156.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT         9088 a         6669 c         5193 g        10359 t
ORIGIN      
        1 ttttttacta atacatcata aaaatgagtc agttcactta accacaaacc tgtaggacgc
       61 tcgatctttt cgtacttttc tacatttgtt aatacgatca atactttcat tttcccatcc
      121 tctttctata aaaattgcgt tattgataac gtgctttcaa gaaattatat aatgggcttg
      181 gcaaataaag ctctttatat gcttcttcaa aaggtttcgt tacaaattca tcctcacggt
      241 tttccaataa tttctctgcc cagtcaggat cgacgatcat ttgctgtcca attgctgcaa
      301 gttctgcata tttcaatacc tgctctacat cttcgcgatt gcggatccca ccgacaccaa
      361 ctaaagggat ctttccttga atcgtgttat ggaccacttc aagaatcgat ttatcgttgt
      421 atttatcaga acgagctatt cgatcataca tatttaatga aacatgcaga taatctagtt
      481 ttgactcacg taatcgttca agaagccaga tcgtgtcttc aaaacggata cctggtgttt
      541 catattcttc tggtgagaaa cgatacccta cgatgaatgg ttttttagca tattgttcaa
      601 ccgctgagaa aaccgttgtt aataattctt caataaaacg ataacgattt tccaagctac
      661 cgccccattg atcctctcgt ctattcgaat gcggagagaa aaattgttgg atcaaatacg
      721 tatttgctcc atgcaactcg attccatcaa atcctgattc aatcgctcta cgtgtggctt
      781 tgccaaatgc ttggattgtt tcaacgattt cttcttcact cattgctcgt ggtgtttccg
      841 catcaggacg ttttgctgca actgcacttg cagatacagg ctgttctcca gataaagttg
      901 ctcgacttgt cattcgtcca ccgtggaaaa tctgaataat cgcttttgcg ccttctcctt
      961 ggattgcttt cgctagctca cttaatcgag gaatcatttc atcatgagcg atgctgagtt
     1021 cacctggcca gccttttccg atatcttgga catttgcagc accagtaata acagcaccaa
     1081 gacctttaga acgttgttta taataagtga tctcatcttc ggtgatcact ccattgaaaa
     1141 agctgagttg tgtcgtcata ggcgacagca tcaatcggtt cttcaattga actcctgaag
     1201 gtaaagtaat tgattgatca aattgtgaca ttgtcattcc tcccataatt cgctctgatt
     1261 aacctatcga gaatcatcta tcattaaacc actcgattag ttgtacatgc aactaatttg
     1321 cttaaaaaat tttatttttt ttgtaactta tctgttgcat cattcattgt ctgggctaat
     1381 tctttacttg cttcatttcc caaaactgct ttgtatgact gatgaagttc ttcccagcta
     1441 ccatcgactt ccttagcaaa ttggcgtcct tgttctgtaa gataaactaa tgacaagcga
     1501 ccttcgctaa ctgttttgac gtatccttta tattctaatt tttcaacaaa acgagcaatc
     1561 gttgatgggg cggaatagag catttgtgca atatcttttt gtggtattcc atctttttct
     1621 tttaatatca ataaaataaa accataagtt ggtgccagac ctgtccttct aaacgcttca
     1681 tcagctaatt tattcaacat gcgatctagc tttttcacgc taaagaaaag acattcatct
     1741 aacgcttttt caattggctt ttccatttca tcccctccat tggcgtacat atcatagtat
     1801 gatttttatt ttttgtcaaa aaacacggaa taatgtccgc ctcctctgtc tagatttgaa
     1861 aaaaagaagt agccatcttc aaagaatttc ggcttgtttt cgacaaaact ccttctggaa
     1921 caccgtgaat cagaggttgt gaaaaagtcg tttagttttg agtatgaaga aaaattttgt
     1981 gaaaatggct ttccacattt ttgcataaat ttggcttaat accgaaaaac tggcttttga
     2041 acaccgttta tatcagaggt tgtgaaaaag ccgtcctgca tcaagtaata agaacagcca
     2101 aaccaaaata gttcctcata ttttgtcgtt ttttgagcta atgccgatgg tcagctgatt
     2161 gtacaccgtt tattgagagg tcgtgagcct gccgttgaac tccgagtcac aaggagcaat
     2221 ttcaaaaaac agcttctcat gttttatgac tatggtgact tgtgatcgag gagttggcag
     2281 gagaatactg tttattcgta aagaaggggc tgtgacaaga cttttgtcac agtcccttat
     2341 ttccttttat tataggattt cgccatttga ttggatgact tgtctgtacc aatcaaatga
     2401 ttttttcttt aaccgtttct ttgtgccatt cccttcgtca tcttgatcaa catagatata
     2461 tccataacgt tttgacattt gtgaagtaga agcgctgacg atatcaatgc agccccacga
     2521 cgtataacca aataaatcta ctccatcttc gatagcttcc ttcatctgct caatatgctg
     2581 cttcaaatag tcaatgcggt aatcgtcttg aacggttcca tcttcttcta ctttgtctat
     2641 agctccgagt ccgttttcag ctatgaacag tggtttttga tacctatcat ataattgatt
     2701 caaagcaatt ctcaaaccct taggatcaat ctgccagccc cattcacttg atggaagata
     2761 cggattttta attcctccag ttattaagtt tccttggacc ccttccaaat tttctggatg
     2821 agcactgact gcactagaca tgtagtagct aaaagaaaca aaatcaacag ggtaggctgc
     2881 taagatttct tcatcaccta cttctatttt taagttgaca cctttttctt caaacattcg
     2941 ttgcgtatac gctggataat atcctcttgc ttgaacatca ctaaagaaca aagaagttct
     3001 ttgctgctca attgtttttt gaatatcatc cggatcacaa gaatacggaa caagaagctg
     3061 atagctgatc atacaaccga cttgtgaacc tttgataatt tcatgacaag cttttgtagc
     3121 taaagcactt gctataaact gatggtgtgc accttggtat ttcgcttgtt ccaaatgggg
     3181 atggttttct tcaattactc ctacgctaac aaaagggtga tgttttacac agttgatctc
     3241 attgaaagtc atccaatatt ttactttatt tttatagcgt ttgaatactg tttcagcgaa
     3301 acgaacgtaa aactgaataa gctttcgatt aggccatcca ccatattctg taaccaaata
     3361 taacggcatt tcataatggg aaagagaaac gactggctca atcccatatt ttagacattc
     3421 gtcaaacact tgatcataaa aaaataatcc cgcttcattg ggtgtttctt catcaccatg
     3481 aggaaaaatt cttgtccatg cgatagacat ccgatagcat ttaaaaccca tttcagcaaa
     3541 taaacggatg tcttctttaa agtgatgata gaaatcattt ccgtgtcttt taggataata
     3601 atacttgctg ttgggatcct gcgccgcttt tacttgctga tgcgtcatca tatgccattg
     3661 atctgtccat ttttctttag gtaaatcgct gtcgaatgta tacacatcag acacagataa
     3721 tcctttttca tctacatccc atgcaccttc acattgattg gccgctgtcg ctccgcccca
     3781 taaaaatcct tctggaaatt ttttcatatc gatcccccta aacttgctag ttttcttgtt
     3841 tgattatttt tcttatgact agtaatgata tttttgaatc tactactatt tgtaacaata
     3901 accattgtgg tcggatcata tccctctttg attaattctt gcaaatctac ttccatcaat
     3961 aaatcgcctt tacgtacttt ttttccatca gatactgcac tttgaaagta tttgccattt
     4021 aaattcactg tatcaatacc gatatggatc agtatctctg ctccttcttc actgactacc
     4081 ccaattgcat gtttcgttgg aaaaactgtt gaaacttccc cgtttacagg actgtagatt
     4141 tttccgtccg ttggaatgat cgcaaaccct tctcctaaag atccactagc aaatgttgga
     4201 tcgtttactt cagataaatc aattatttca cctgctgcag gagtacttaa cactatatcc
     4261 tttgaggatt cttcttcgat ttcatccttg tagccgaaaa gaaaagttag catacaggaa
     4321 ctgatcatcg ctaagaaata actagcaact agccaaacaa atccttcacc aatataaatc
     4381 gggaaagaca aaatacttct tgtagcaact gccaaagcac cggctcctgc ggcaccagct
     4441 actccaccaa atatagctcc acatatacaa gcaatgtaaa atggtcgttt atatttaaga
     4501 gtcacaccat aaacagcagg ttcagtgatt ccaaataatg ctgtgataaa tgcagataaa
     4561 gataaacttt ttactttctt atttttcgtt ttcaaaaata ccccaagtac tgctcctgct
     4621 tgcgcagcta cggcagtcat acatgctgga cctagcgtat cacgaccgta gaaagaaaga
     4681 ttgttggtca ttactggtac aagtccccaa tgtaacccaa aaatcactaa cacttgccaa
     4741 aatccgccga taacagcacc agataaaaca gggctaaagt tataaacagc taaatatcca
     4801 gctgcaatca gctgacttgc ataatctgca attggaccta caactaataa agtaattggg
     4861 accatcacga tgatacaaat gaatggtgtc aataaatttc gaatactttc gtgcaagctt
     4921 ttgcgaatct taggctcaat ataagaaaga acccatacag ctaaaattgc tggaataacc
     4981 gtacttgtat atcttgctag aatcacgggc atccctagaa atcgcaaggt tatgctatca
     5041 ctatatgcac tgacaatcgt aggataaacg agagctgctg caataaccat agcgatgaaa
     5101 cgatctgtat tgaacttttt tgcagcagta tatgccaaaa atataggcaa gaaatagaaa
     5161 aatgcatcag ctgctgcatt caaaatgata taagttcctg attcagtagt taataaattc
     5221 aaactcgtac ataaggcgag tactcctttg atcaaaccac ttcctgccaa gacgcctaaa
     5281 gtcggggcaa aaacagcggc gatcatgtct ataaaagagt ttaaaatgcc ttttttctca
     5341 gttcctctct ttccactaga tgcatcatct tctagtttca tttgtttgga taatacgtca
     5401 tacacttcat ttactgcatt tcctactacg acttgaaaca ttcctccagc ttcgactaca
     5461 gtgatcacac cttctagttg ctctaccttc tccttatctg ccttcttatt atcttttagg
     5521 gtaaagcgta accttgttgc acaatggtaa acattgctga cattttcttc gcctccgaca
     5581 aatcggacaa tatctttcgc taattgtgaa tagtccattt ttacttcccc cttgttaatt
     5641 aatgccgtag caatccttct tacaagataa gtatagttct tagaaaacgg tttctccatt
     5701 caattagtac cccttctaat agcgaacgaa actgtacaat agtgtgtatg gtttacttag
     5761 ccgagcaaat cactcactgc ttgaaagaaa acaagtatat cgtttctctt atagtcaaag
     5821 gagacaaaaa aaacacgctt gatcttcctt tgctgatgta taagtggtat ctcaagctga
     5881 tactctgtga tttttgtttc tacttggctt ttgataagac agcacaacaa agtctcggca
     5941 tttaacggaa gcataactag gtcagaacta ggagagatta gttgctgaat ggaatctatc
     6001 tttttatgga tttttatttg attttcttct ttgtagtaat gatcaagcag ttttgttttc
     6061 tttttttctt caactatggt cagcttttca cgcaaaatct gataaatctc cgaagattca
     6121 aactcatcta gaacactagg tagataaatc aaatcatccc actcaaaaat aagctcttgc
     6181 tcagtagtca aaattgcatc atattccgtt attttgtttg attgattttt gaaaagatac
     6241 actggttcta tccggcattc tttgatccac atgttcatac tttgttgaat ctgttttttt
     6301 agtgtattca aaatactaat tggctggtta ctaacaatca gtatctcccc ttgttttcga
     6361 aaaggattca acgcttcggc aagatacaaa accaaataag cgctttcagc taaattggga
     6421 cgtaattgaa aatgtattgg gaagaaacgc cgaattaaat aaatttctaa aggataacgg
     6481 gagatgatct tttgtacaaa atgattcatg gtattatgtc cggcatccat cctcgttttc
     6541 atttgtttga tatgggttag caaaaattct gtttcatgga acaataacga aacaggaaga
     6601 tctaaaaaac gaataatcgt ttgctcaaat gctcttagat tcttctctat ttctttgttt
     6661 ttttcatcat taatcatcaa ataatttaac tctaatagtc gattttcaat tgcttgtttt
     6721 tcttgattgg aaaaagaata gggtagttct gatgataact tttttgtgag ggtctctacc
     6781 atcggaataa ataatgtata cctgtttgtt ttttctaaaa gttcttctct tcgaagaatg
     6841 gtcaaaacga gataccgaga aaatcgtatg aagtcctctc ctgaaacaat ataattatat
     6901 gatataagaa cctcttttaa tgtcttttgg acagtcggca tgatattgga aaaaattgta
     6961 ctgatttgtt gaggcaacct tgcgtgttgg atcaccgttt ctgttccaac aagagataag
     7021 aaaattcttt ttgaattttc ttcagcaatg atttttagtc cttttgtcga cgaaacttcc
     7081 agttctaatt taggattgcg ctcgatccaa cgcaaaatcg tttttatttg taatgatacg
     7141 gctgtttttg aaagaaagaa aatgtctgct atcttttgca tactgacata ccctttttga
     7201 aacaataagt acgtcacaat agttgaagct cggaaatcca tgctggaata gattttatgt
     7261 ttcgtcacct cttgtatgat ttgctgttta gtttcagatt caattgattc taaaaaataa
     7321 cctgaacgtt tatttgactg gataatgatt ttcttctcgt agatagcaag catatttttg
     7381 atttcagttt ggatcgtttt cttgttggta cctaaagctt cagctaattc ttcgcttgtt
     7441 atccaatgat tcttatttct taatactaca agaatttgga gttgtcgttt atttaagtcc
     7501 atttgcttac acctcctctt tgttataaac atacaggaaa aacctctcaa atgacaatag
     7561 tcacttgcag aggtttaaaa atcatttgaa gaaagatcta tattcaaggt ttttaccatt
     7621 agactggata atttgtttat aatattcaaa actatctttt ttcaatctcc taaagcttcc
     7681 gctcccatca ttttgtctat ccacataaat aaagccatat cgcttcctca tttcccctgt
     7741 tcctgccgat acgatatcga ttggtcccca ccataagtaa ccaatgattt ggcaaccatc
     7801 ttttattgcc tcagcaattt cctgaagatg tatttttaga tactcacaac gttttggatc
     7861 gtggattcga ttcaattcat ctggtgcttc atctagtcca atgccatttt caactatgaa
     7921 taatggtaat tcataacggt ctgtcaattc attcatgaca taacgaaacc ctagaggatc
     7981 aattggccaa ctccaaggtt cgggagattt ttccttcaaa aagggattat cgaaaccatt
     8041 tgctccgcca gtatctacgc gcatttctgc ttctttttta tagacagcgc tacgataata
     8101 actaaatgca atataatcta ccgtgttagc ttgcaagtac tgttcgtctc catctaacat
     8161 tactggtgca cagtcttctt ccttccatat cctgtaaata taagctggat aatgccctcg
     8221 gcacatgaca tcggagaaaa accatgaatt gcgtttaaat tgcagtgaac caaatacatt
     8281 ctctggatca cagtcatatg cataagtcgc taatgcagaa aagctacaca taatacccat
     8341 cttagcattt ggatcgattt tctttaataa ttgagtagca cgggcgttcg ctagaaacat
     8401 atgatgacag gcttgataaa tctctttttt tgttagtgta gcaatgggtt caagtttatc
     8461 tctagcaggg aatgtaacca atccagcagc tgcataaggc atacgaaaag cattattgat
     8521 ctcattaaag gtcatccaat atttcacttt cccttggtag cgttcaaaga cggtctgcgc
     8581 ataaaattca aaaaagtcga ttaattttcg attttgccac cccccatact ctcctaccaa
     8641 tgctaagggg gtttcataat gactaagagt aattactggc tcgatatcat atcgatttaa
     8701 tgtattgatt agtctgtcat aaaatacgag accagcctca tttggcttct gttcgtctcc
     8761 tcttggaaat attcgtgccc atgagatgct tgttcgaaaa gcttttaaac ctaactcctt
     8821 cagtaaacga atatcctctt caaatcgatg ataaaaatca atcgcttcat gagaaagata
     8881 atggagtgat tcgtctaaag caatctccca aatctttttt tcttcattcc actgaatact
     8941 aggatgctgg ttcatgatcc cgataagcgt atctgtcaca ttaggtaatt tgccgtcttc
     9001 caaccatgca ccttcacatt gattagcagc tactgctccg ccccataaaa aattttctgg
     9061 aaatatttcc tgatccataa ctatctctcc tcgctatcaa caatttctta cttgaagtat
     9121 agctcgattc atcaaaacaa gatattctaa ctatttcggc agaagagaag aaaaaaattg
     9181 aatgctactc ttcattttta atatttatat ataacaaata cataagacga tccccgaaag
     9241 aaaacactcg ttaaccatct aagcaaacga tttattttac ttccaacaca aatgcttcat
     9301 aaggtcgtaa atcaactttg ccgtctggta actgtttcag aggataattt gacacaataa
     9361 cccttaaagc ttcacgtgga acagtgaacg tctgcttttc actggacata ttacaaataa
     9421 cgagccattg ctggtcttca tatatgcgag aataggcaaa gacatgctca ggcgtctttt
     9481 ccactaattc atagtctccc cagacgatga tgtcatgttt tctacgcaaa tcgatcaatt
     9541 tcttgtacat ataaaaaaca gaatcttgat ctgctaacgc ttcttctgca ttgatttctt
     9601 tataatttgg attgactcga agccatggaa ttccagtagt aaacccagca tgttcacggg
     9661 cgttccattg cataggcgtc ctagcattgt ctcttccttt cgcattgatt gattccaaga
     9721 tttcctcttt tgtaaatcca gaagaaatcc tttcgttata catattgatt gtctcaatat
     9781 cttttgcttc cgcgatatca tcgattgggc aattcgtcat gcctaactct tctccttgat
     9841 agatataagg ggtgcctttc atcatatgga gcaagatagc aaacatcttg gcactttctt
     9901 ttcgatacac atcgtcattt ccccaacgag aaacaattcg cggaagatcg tgattattcc
     9961 aaaataaact attccatcct tcatcaccta aagatgtttg ccatttggat aatacttttt
    10021 tcagttcaga gatactcaag ggtttcaaat cccatttgtc tttgccttct tgctgatcta
    10081 atcctatgtg ttcgaattgg aagaccatgg ataattcatt tcttttagga ttggaataaa
    10141 gtttagcgat ttcggtagtt gctccccatg tttcgccaac agtcatcaga tttttgtcac
    10201 caaaagttgc ttgattcatt tcctgaagat actcatgcag tttaggtcca ttacttgtga
    10261 tttctttatc cggttctttg ccaatcatat cgatcacgtc catacgaaag ccaccaatac
    10321 ctttatcgat ccagaaattc atcatttgat aaatctcttg gcgaacctgt ttatttttcc
    10381 aattcaaatc aggttgacgc ttgctataca agtgcagaaa atattgcccc gttttttcat
    10441 ccaactgcca agcagaacca ctgaatattg agcgcaatct attaggctct tttccattga
    10501 ccggatcacg ccaaatataa tagtcccgat aaggattttc tttattttta cgggcctcaa
    10561 tgaaccacgg atgttcatca gaagtatgat tcaccaccag atccatgatg atacggatat
    10621 ttcttttctt tgcttcttca atcagttctt ccatttcttc catcgtaccg aattctgcca
    10681 tgattgcttg ataattactg atatcatacc cattgtcatc attcggagac tcgtatactg
    10741 gagaaagcca aattgcatca atacctaaat acgccagata atccaacctg gaaataatcc
    10801 cccgcaaatc tccaatacca tcttgatttg aatcttgaaa acttcttgga tagatttgat
    10861 aaacaactga atggtgccac catttttgtt ccatgatttt cctcctataa atggatgttt
    10921 ctattttttg cttaatcatt tattttagtc tctctcacta aaaattgtaa cgctttcaaa
    10981 tagctattgc accttgttat cggtaacaga ataaaacaaa aaaacagctg aggaaattcc
    11041 ctcaactgtt ttttgaaagg attgatttat tcagggaaga atgctgattt cacaacttct
    11101 ttataaacgg tgttcactca gctgtccctc ggtattagct caaaaaacgg caaaatataa
    11161 gaaactattt ttttcgtttt tttctctaat actcaggcca gaacactgat ttcacaacct
    11221 ctttatttca cattcaatat ccacatgaat cccatgaata caaagaacag accatacatg
    11281 atgggagata cttcttttcc tcttttagct gctaacatgg taattggata gaagataaaa
    11341 cctaatgcaa tcccatctga aatactataa gtcaaaggca tgccgatcaa gatcaagaat
    11401 gaagggatcg caatctctaa gtttccccat tcgatttgtc gcaaagattg tgccatcaat
    11461 actcctacca taatcaatgc tggtgcagtt acttgtgaag taacaactga tagtaatgga
    11521 gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat acctgaacgt
    11581 cctccaacgg cgataccagc tgatgattct acaaatgcac caacaggaga agtacctaat
    11641 aaggaacccg caagcatagc tgttgaatca gaagctaaag ctttccctac acgaggcatc
    11701 ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc tgctgtatca
    11761 aagaaagtca caagcaagaa tgtcagcact acgacccaca tttgaagaga attgatgtcc
    11821 cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc ggcaacaatt
    11881 tcagaaggca ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc tgccatcccg
    11941 atgaagatgc cacctggtac acgccgaaca agcaagatag cagttacgac taatccaaaa
    12001 atcgtcaacc aagtagaacc aacgttgagt gatcctaatc ctactaatgt agattcattc
    12061 gaaacgatga tcccaccttc gctcagacca aggaaagcaa taaatagacc gataccgcca
    12121 gaaatcgcat atttcaaatc tgctgggatc gcatcaatga tcatttcccg cagcttgaag
    12181 attgtgatca aaataaaaat caaagaagca acgaatacgc cggctaatgc tgtttcccat
    12241 ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag cattgatccc aagagcaggt
    12301 gccgttccaa ttggataacg ggctaatacc cccattaaaa tacagccgat cgcactggct
    12361 aatgcagtcg cagtaaacac tgctccttca tccattccag aagcacctaa tacagttgga
    12421 tttacaaata agatataagc cattgagata aatgttgtaa atcctgccag tacctcacgt
    12481 tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa gtattttttc cttcatcttc
    12541 catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt tattaataaa
    12601 acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt cggattttaa
    12661 gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat gaacattcac
    12721 tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta gtctcaaaaa
    12781 tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga attcttttgt
    12841 atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt ttccccagcc
    12901 ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac tatgttttgc
    12961 tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt ctcctccttt
    13021 ttattcaatc agacgactca gcttttccta taacaaaata attgccttca gggtccgaaa
    13081 aattgaaagt caagacgcca ttgttgtctg tgatttctcc tggttcttcc atgttctcgt
    13141 agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct aatacctctg
    13201 gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct tttgagatgc
    13261 tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt tctgcaccaa
    13321 ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc atcacacgtg
    13381 ttttttgtat catcgtttga cccttctttc tatttgattt tctttcctgt ctataaactg
    13441 attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa gaaaaataaa
    13501 gcaatagttt ctaggattgc tttcctccac aaactttttt tacaaaaaaa aaacggcaat
    13561 gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta
    13621 gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga
    13681 ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg
    13741 cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg ccagttgaga cagcgacaga
    13801 tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg
    13861 cacctaaagg caggaatgat tcgtcacctt gggcttttcc taaatgcaga tttcctgcga
    13921 tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga
    13981 tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag
    14041 cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact
    14101 gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg
    14161 ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga
    14221 taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat
    14281 ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag
    14341 atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc
    14401 ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc
    14461 tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc
    14521 ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat
    14581 ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat
    14641 actcactagg aaaaaatctg tttctttatc ttttagctct tctgtaagtt tttctagtgc
    14701 ataagcttgt gtcaagtaat cagctaaatg ctccggtaat tatcttttcc aaatgtatct
    14761 gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga
    14821 atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca
    14881 aaattccaat cttcgctaac attcttgcat actttattta aaagatattc caattgtttt
    14941 gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg
    15001 atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt
    15061 tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca
    15121 gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt
    15181 tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg
    15241 atattgctaa tagagtctag cgctcgggca atcattccga tgtctcgaag gatttcagcc
    15301 atttcatccc acctttattt tgtttgtaag actagtttac ctctttttgt tgcaaatgca
    15361 actattttta ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct
    15421 tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact
    15481 gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca
    15541 tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa
    15601 aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa
    15661 ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca
    15721 cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg
    15781 tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta
    15841 cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt
    15901 cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt
    15961 tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt
    16021 attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta
    16081 atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat
    16141 aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa
    16201 atggcgttca aaagcttgca tctgcggtaa taagccccat caaacgaaaa atatgacgag
    16261 ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca
    16321 ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag
    16381 aagctgtttc tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc
    16441 tcggtaaacg gtgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt
    16501 gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc
    16561 tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat
    16621 gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac
    16681 gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg
    16741 tttgtaccga cgttgccata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg
    16801 atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata
    16861 ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa
    16921 aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga
    16981 ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc
    17041 ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa
    17101 atactttttc atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc
    17161 caatcttatg cttgacaatt tcttttactg ctttatttgc tggaatcaca ttatggcgaa
    17221 gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta
    17281 aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt
    17341 cttctttcac acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt
    17401 ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga
    17461 tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc
    17521 ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc
    17581 caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct
    17641 tcaagatatt ttctttgaaa ggtagatggg acccgtcata catgacagaa gtatatccgc
    17701 tatcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgt aagactgcat
    17761 caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat
    17821 atttagcggt atcgacactt gtttgaataa tgattggcga acccatttct tttgctgcgc
    17881 ctagcatggc aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca
    17941 actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta
    18001 tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt
    18061 ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc
    18121 tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaag
    18181 ctcttttcta aatattggat ggcttgatcg ttctcatgca attcagaaaa tttaagtcca
    18241 acttgttcat aaagttccgc taatttatgc ctatcttggg tagtggaaat ctcttgtttc
    18301 aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct
    18361 tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc
    18421 cctactttca attaaaacgc tttcaaaact gggcttaaga tccaggcata taagagtgct
    18481 gcggctactg tatctacatc ggttgctgta gcattgatga atcccatcga attaaagatc
    18541 gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc
    18601 gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa
    18661 ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca
    18721 ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cggcatttgg gctataagga
    18781 aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct
    18841 cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa tatatacaca
    18901 cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat
    18961 aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc
    19021 acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata
    19081 tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca
    19141 aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta
    19201 cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca
    19261 ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc
    19321 gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga
    19381 gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa
    19441 cccaaatcat tcattgcttg atcattgata gattcaatag aagggatgat cccttgcata
    19501 tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa
    19561 ccggcactca atacttgaaa tcctaggagt gtttttaacg tgccagagat gacttgtcct
    19621 gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc
    19681 tgagtcaaaa tattttcgat gataaaattg ataacgctca tgtttttacc ctcttttctt
    19741 tttgtattta gataactccc ttatcttgca gaactggggt gattttttct tcaatttcat
    19801 tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact gcgaactttt
    19861 cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg gaaactgcgg
    19921 aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa acatcctcag
    19981 cagacatctg tgctgcaaaa ctacttccta aacctgcgcc gcatacgaat aaaatgttca
    20041 atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact gaaaaaagca
    20101 gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc tcttcgttgt
    20161 tgatcaattc aattagttct ttcatgatgt tcagatggga gtaggagtct actgctgcca
    20221 aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt ggttgctcaa
    20281 tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca tgggctagtg
    20341 ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga atcatcgctt
    20401 cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct agttcaatcg
    20461 attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc aaaatatctt
    20521 ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg aataattttt
    20581 ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg acatctatta
    20641 tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt agttcttcat
    20701 ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc ggatcgacaa
    20761 ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga ttgacatcca
    20821 atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg atctgaggaa
    20881 acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg ttacagacaa
    20941 cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc gtcgaaaagt
    21001 ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact tcttcgatcg
    21061 taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg atgttttctt
    21121 tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca gccatgtgct
    21181 tatagagatt ttcgaataag gcttcttcct ttagatggaa aggtacgtgt gtctcgttct
    21241 ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg attgaattgg
    21301 agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt tctacttgcg
    21361 ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa aagctactgt
    21421 cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc ctgattcgct
    21481 ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct tcttgtttca
    21541 acaacttatt ggaatagatc tcctgtattt ttttgatttc ctccaaagga aaataatgga
    21601 gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag tcggtcattc
    21661 ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct gaacctttat
    21721 aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc agtgttgctc
    21781 gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct tcttcttttt
    21841 tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca aaaatacgtt
    21901 cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat cgagcttgct
    21961 tttccttttc cagcttcaac aaaaaacctt tgttgcgaat tgtacggatc tccggaagct
    22021 ttttactgct caaccagata ttcgcttcaa gaacttcctg acgaatcgtc cgctgactga
    22081 cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc atgattctgt
    22141 gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt caagtatatg
    22201 gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa gaaagtaagc
    22261 aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa gacatacgcc
    22321 tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa gctgtcctgc
    22381 atcaagtaat aagaacaact aaactaaaat agtgcttcct attttttgtt tggttgttct
    22441 tattatcgca gatgcaagct tttaaacacc atttagtgga gaagctgtga cagagatgtt
    22501 cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta caaaatcgtg
    22561 ccttattgcc gataggctat ctctggaaca ccgtttattc agaaaaagga gctgcgacaa
    22621 ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa tcggttgaat
    22681 aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata tctgagaaaa
    22741 atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat cgactgaata
    22801 atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct gtcgataatt
    22861 tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg ctttgttttt
    22921 ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc tggctttttc
    22981 aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga tttccaaaaa
    23041 ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg atttcagcct
    23101 tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta ctgacaaaac
    23161 agctaatgca agaaaccccg tttgctcata actagaagga cctgtacttg gaccataata
    23221 cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat atgaacgacc
    23281 gccaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat gaccacccgc
    23341 gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca aaatgattgc
    23401 taaataattg attttttcat tgtcttcctc gttatctttt attacaaaaa actattttta
    23461 tcatagcctt gctaaaagga agagtcaata accagatgca taaactgttt ttttagcaat
    23521 tttgtcttat ttagtcattg agtaacatcg gacagatgta tgtgtcggca gtccataatt
    23581 cctctatctt cagcatgaac aagcttgcag acaagagatc tgcgcttcct ccaggagaga
    23641 tttggttttc ctgacaaaag tgcaacagtg ctgtaaatgg acgatttggt aaagcacttt
    23701 gactagcagc atgagcaagt ttttgcattt ccactaacgt tttctgatca ctgcgatgca
    23761 atacgcatgt gtcatctagc gaactcatca aaactaacaa catatgcatt tgtttctgta
    23821 catatgaatc attatctagc tccttagctg cttggattgc aagcctgata tgcggatacc
    23881 ctaatatcgc ttcttcatat gcaccattta cgccatattt ttgcttcacc ctatgaccat
    23941 gagtggtttt tttatgacga tattttgtat caggaaaagc agcaagcttg ctgacatcat
    24001 caagtatttt cgataaagaa aaaggctgtt ccatactgat tttttgtgca atgacactga
    24061 tcaatagccc cattgcccaa atcgctcctt tatgggtatt gatgccattt gtagcaatca
    24121 gcattgcctt ttccgctttt cgcccaatct cagcaattct ttctcttaac tcctgatcaa
    24181 tagcataatg ccatgctatt tgagccattt cttcaaaaaa aggagttagc gtcttagcag
    24241 acttatccaa aatgcgccga ccttctttta cggcttttgc gatcgtatgg aaattatcat
    24301 ctcctaaaac catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcga
    24361 ttcctatatc ggctcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga
    24421 cgatttctcc atttttctgc aaagctttca cgattcgcag tttgtgttct ggtgtcgttc
    24481 gagcaaagac gtcgactttt ggtacttgtc gaatcaactc ttcttcagac ataagatcca
    24541 aatcgatccc ttctaaaact ttagcagtat gttttaagcc aatttgttcc gcgatcgctt
    24601 ttgctgtatc tttgtgatcc cccgtaatca ttttcactga gattcctgct tgttggcatt
    24661 cctttacagc tttgactgca ctttcttttg gcggatcaat caacccagca atccctgcaa
    24721 aagttaattg ggttaaagac tcatgcgtga gttcctgttt cgaatctact tttttgtaag
    24781 caaatcctaa aacacgctga ccttttttcg caaacgctgt ggctgtttga ccccatgctt
    24841 cttattctga agaagaaaga gttgatttag ccaataatac ttctggcgca cctttgacgt
    24901 aaatcacccc tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagagctga
    24961 acggaatttt tgcttctatt tttttcaaag gcagatcggc tgtttcggtg tggcgaatca
    25021 gtgcaagttc tgtcggattg ccacttaaat tttctgtttt ctgttcttct ttcgtcttta
    25081 attcctgaca atttttcata ataaacagta cgcgagtttc ttcctttgct gctacatcca
    25141 caactgtcat ttcatttttt gtcaatgtcc ctgtcttatc tgaacagatc actgtcatag
    25201 aacctaatgt ttctacagaa ggcagtcctt tgatgatcgc cttttctttt gccatttcat
    25261 gtactccgac agataggatc atggtcaaca cagctggcaa tccttcgggt accattgaca
    25321 ccaccaaagc gatcatagct gaaagaagga tatgccaatc catgccatag cggaatgttg
    25381 taaaaacaac taagaacaag atcaaacaaa gaatgccttg aaaaatttgt ttgttcaatc
    25441 ggttgatctt cttgatcaga ggcgtcgttt gttgttcgac cgattgcagt gcttgattga
    25501 ttttaccgat ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt
    25561 gtacaagcgt tcctgaaaaa gccatatttt tttggtcacc gattgattct tctgctgcca
    25621 aaacggaatg atttttttca gatgcttctg attctcccgt caaaatcgat tcttctacta
    25681 gtagatcatg tacttcgatc aaccgaacat ctgctggaac gacgtctccg gcacgtaaga
    25741 caatgacatc tccttttact agttctttcg cagggatttt ttcttgcatc ccgttcgata
    25801 gtactatcgc ttcttgtccc atcagctgct tcaaaccatt caacgattct tctgcttttc
    25861 tttcttgcca ataactaacg agtccgttga cgatcactac gaataaaata atacttcctt
    25921 cgatgtattc gctcgaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca
    25981 gatcagtaaa gtgttttgct atttttttcc atcgttttgt ctgctgtctt gcttctatct
    26041 cattatatcc ttgatcagtc aggcgtttct gccgttcttc ttgcgacaag ccttctgttg
    26101 atgtttgata gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc
    26161 ctcctgttta attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat
    26221 ttcagggggg tctgaaataa ttcgttttgc agaatgtatc tgggcctaac tgatatttct
    26281 attgcttatt cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg
    26341 aataaagcgt gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa
    26401 caaagcaaga tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac
    26461 atgtgcacac aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa
    26521 aagctgaaaa aacaatcgac tatcagctac aaaaaaatgt agacaaagac cgacaaaaaa
    26581 taacatccct gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa
    26641 ccaatggggc aaacttttct cttttgctgt ttgatataaa atcctgctag tactgtagac
    26701 tgcactattt gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc
    26761 gctttggcaa tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca
    26821 atgccatgga acaacaaata aaatcgacaa aatcgttcct atatagaaaa agaggatacg
    26881 ccaaggcaga cagttgattg catttttcaa attcttttgt gggtcactcg tctcaccaac
    26941 tgttaatccg atcatttcaa ttccagcaaa actgaataaa accataggaa atgaagcaat
    27001 cattccagca aagccaaacg ggactaattc attgagctgt ccaaaatttg ctttgattcc
    27061 aatagactgg gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa
    27121 aatccccaag atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc
    27181 aacaaggttg actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac
    27241 atgtacaaac cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc
    27301 aagccagcaa agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata
    27361 gtcaacgaaa tcactgatag agttaaatgt actatttgct aaaaataaat cacccatgac
    27421 ccgcatcaaa ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc
    27481 tgctgatgca attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa
    27541 tgcgattaac tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa
    27601 gttcaccttt ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta
    27661 ttttttcaat acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta
    27721 gattgatcca tgtcccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata
    27781 aaacaataac gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa
    27841 atggtcgatt caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga
    27901 catagacgat acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga
    27961 cgccaggaat cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa
    28021 taacggattt ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg
    28081 caccttttct agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt
    28141 ggactgctac accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac
    28201 caagaatttg gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt
    28261 ttggtacgac atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta
    28321 acgcggcaaa tactccgcga atatacgttt taactggatt tttcaaccga gtgatataca
    28381 cagaggacat ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg
    28441 atgcagtcgt agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg
    28501 gtacgatccc aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca
    28561 cgtacaatcc taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat
    28621 tcaaaaaagt gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca
    28681 aaccaagcgg cacattatcg ataatattac caaacaacgg agcaagtgct gaagcgacca
    28741 tcaccatccc aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt
    28801 ttcctagagc attcgatacc caaagttctg gaccaccttt ttggggaaac attccggcaa
    28861 attcaccaga gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa
    28921 aaatcatcgt ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg
    28981 cgcaggtcat tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca
    29041 tctttattcc ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga
    29101 acagaaatga caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt
    29161 gaacgatctt agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt
    29221 ttataaaaaa gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt
    29281 tgatgaagca aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat
    29341 cagttctatt ggatgatttc gtttagtttc ttttccattg ctttggcgat ttcttttgta
    29401 taaaaattga aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga
    29461 gtgattttct gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga
    29521 atcggagaat caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca
    29581 ccataaacat cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg
    29641 atagcgttca aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca
    29701 ggatgattca atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg
    29761 agaaattctc tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg
    29821 gtcacattca atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct
    29881 tttgatcctt ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac
    29941 acatatggcg cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct
    30001 ccagcagcat aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct
    30061 gaaatagctc tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca
    30121 tgatgttctt caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt
    30181 agaaacagtg aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc
    30241 ccttcatctt ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct
    30301 tcttctgtcg ttcctacgac agcaaccaca cctagaattg ggattttttg ttcagctaat
    30361 tcacgaattg ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata
    30421 gcaaccatct gatctaagcc tactccgcaa atatctaacg ccttcatcca agaatagtgt
    30481 tttgtttgag gcaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa
    30541 gctgctttga cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct
    30601 tcaacagata aattcaaaag ttcccactca gacatttttt tcacttgttc aggcagcact
    30661 tccttgacag ctaaaggaat cgatttgatg cagcgggcgt accataatcc ttcaagatta
    30721 gcaatagaac catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca
    30781 aaatcctgtc ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca
    30841 ttatttgggt tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca
    30901 gcattcattt gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat
    30961 cgctgactga cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga
    31021 gtgagcttat ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat
    31081 ccaagatgtt catctaccat cttgttcaac aacattttat aaactggtcc attttcagct
    31141 ttgtcgccta taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt
    31201 tgttggtcaa tgtctcgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc
    31261 aaaaatgaga aatttttttt tataacatca aatccacgta gatttttac
//
LOCUS       DACEDD010000033        31185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEDD010000033 DACEDD010000000
VERSION     DACEDD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.033"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..945)
                     /locus_tag="IWO45_001681"
     CDS             complement(<1..945)
                     /locus_tag="IWO45_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0333157.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIA"
     gene            1639..2025
                     /locus_tag="IWO45_001682"
     CDS             1639..2025
                     /locus_tag="IWO45_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0333158.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            2123..2314
                     /locus_tag="IWO45_001683"
     CDS             2123..2314
                     /locus_tag="IWO45_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0333159.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2828..3160
                     /locus_tag="IWO45_001684"
     CDS             2828..3160
                     /locus_tag="IWO45_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0333160.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            3070..3273
                     /locus_tag="IWO45_001685"
     CDS             3070..3273
                     /locus_tag="IWO45_001685"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0333161.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            3457..3612
                     /locus_tag="IWO45_001686"
     CDS             3457..3612
                     /locus_tag="IWO45_001686"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0333162.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3888..5228
                     /locus_tag="IWO45_001687"
     CDS             3888..5228
                     /locus_tag="IWO45_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0333163.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(5469..5876)
                     /locus_tag="IWO45_001688"
     CDS             complement(5469..5876)
                     /locus_tag="IWO45_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0333164.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(6065..6892)
                     /locus_tag="IWO45_001689"
     CDS             complement(6065..6892)
                     /locus_tag="IWO45_001689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAQ0333165.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            7201..7419
                     /locus_tag="IWO45_001690"
     CDS             7201..7419
                     /locus_tag="IWO45_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0333166.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            7440..8075
                     /locus_tag="IWO45_001691"
     CDS             7440..8075
                     /locus_tag="IWO45_001691"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0333167.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(8268..8507)
                     /locus_tag="IWO45_001692"
     CDS             complement(8268..8507)
                     /locus_tag="IWO45_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333168.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(8531..10054)
                     /locus_tag="IWO45_001693"
     CDS             complement(8531..10054)
                     /locus_tag="IWO45_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0333169.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(10072..10194)
                     /locus_tag="IWO45_001694"
     CDS             complement(10072..10194)
                     /locus_tag="IWO45_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0333170.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(10224..11207)
                     /locus_tag="IWO45_001695"
     CDS             complement(10224..11207)
                     /locus_tag="IWO45_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0333171.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(11231..11380)
                     /gene="rpmG"
                     /locus_tag="IWO45_001696"
     CDS             complement(11231..11380)
                     /gene="rpmG"
                     /locus_tag="IWO45_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0333172.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(11401..11778)
                     /locus_tag="IWO45_001697"
     CDS             complement(11401..11778)
                     /locus_tag="IWO45_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333173.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11810..11989)
                     /gene="rpmF"
                     /locus_tag="IWO45_001698"
     CDS             complement(11810..11989)
                     /gene="rpmF"
                     /locus_tag="IWO45_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0333174.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(12004..12273)
                     /gene="rpsN"
                     /locus_tag="IWO45_001699"
     CDS             complement(12004..12273)
                     /gene="rpsN"
                     /locus_tag="IWO45_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0333175.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(12796..14568)
                     /locus_tag="IWO45_001700"
     CDS             complement(12796..14568)
                     /locus_tag="IWO45_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333176.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(14522..16276)
                     /locus_tag="IWO45_001701"
     CDS             complement(14522..16276)
                     /locus_tag="IWO45_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333177.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(16386..16955)
                     /locus_tag="IWO45_001702"
     CDS             complement(16386..16955)
                     /locus_tag="IWO45_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0333178.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16973..18397)
                     /locus_tag="IWO45_001703"
     CDS             complement(16973..18397)
                     /locus_tag="IWO45_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0333179.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(18399..19067)
                     /locus_tag="IWO45_001704"
     CDS             complement(18399..19067)
                     /locus_tag="IWO45_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0333180.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(19198..19782)
                     /locus_tag="IWO45_001705"
     CDS             complement(19198..19782)
                     /locus_tag="IWO45_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0333181.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(20113..20346)
                     /locus_tag="IWO45_001706"
     CDS             complement(20113..20346)
                     /locus_tag="IWO45_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0333182.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(20361..21311)
                     /locus_tag="IWO45_001707"
     CDS             complement(20361..21311)
                     /locus_tag="IWO45_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0333183.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(21308..22150)
                     /locus_tag="IWO45_001708"
     CDS             complement(21308..22150)
                     /locus_tag="IWO45_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0333184.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(22283..23008)
                     /locus_tag="IWO45_001709"
     CDS             complement(22283..23008)
                     /locus_tag="IWO45_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333185.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(23469..23783)
                     /locus_tag="IWO45_001710"
     CDS             complement(23469..23783)
                     /locus_tag="IWO45_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0333186.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(23799..25394)
                     /locus_tag="IWO45_001711"
     CDS             complement(23799..25394)
                     /locus_tag="IWO45_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAQ0333187.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(25417..26307)
                     /gene="iolE"
                     /locus_tag="IWO45_001712"
     CDS             complement(25417..26307)
                     /gene="iolE"
                     /locus_tag="IWO45_001712"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAQ0333188.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(26324..27349)
                     /gene="iolG"
                     /locus_tag="IWO45_001713"
     CDS             complement(26324..27349)
                     /gene="iolG"
                     /locus_tag="IWO45_001713"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAQ0333189.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(27373..28380)
                     /locus_tag="IWO45_001714"
     CDS             complement(27373..28380)
                     /locus_tag="IWO45_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0333190.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(28493..29653)
                     /locus_tag="IWO45_001715"
     CDS             complement(28493..29653)
                     /locus_tag="IWO45_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ0333191.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(29729..31183)
                     /locus_tag="IWO45_001716"
     CDS             complement(29729..31183)
                     /locus_tag="IWO45_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAQ0333192.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         9789 a         6246 c         4822 g        10328 t
ORIGIN      
        1 agctatttcc tttacagtaa ttcttttata gatatttaag ccgatgaatt gaacgacaga
       61 agaaattaag ggagaaagat gctctatctt agtttttccg atttccttag tataataaac
      121 aatcccagaa tccctcactt ttaagacctc aggtaacgat acagctaatt cgttttcttg
      181 tatcaaagca tctctacttt gatacgcttc tattatatcc attcctaatg ttatagacgt
      241 ttgtgctaac ttttcaaaaa caatgataga ataatttttt tcagaccgaa tcgtatctcc
      301 actaacactt ggaattattc cattactcag attaaaaacc atgttttcta aatttcgaat
      361 atctccattt ttaacatatt ccagtatttt attttcataa agaaacagat atatttctgg
      421 atcgtaattt tgtgataaca gattatctat tttgatttgc tcgatagttt tacttagatt
      481 tcctgtatat tcaattaacg gttcatggaa tagatcttct atttttccag taaaaaaata
      541 atgaactaaa atcagaatat ctcgaatgtc tcctaaagaa tacaaagtca aattttgcaa
      601 tgaattatag agtcgttctc tatcagccat tgcaacattc aaattgttca tttttagttt
      661 aaaaaaatct ttatcaatat tatttgctct aaatggacca aataagtaat atctggtttt
      721 aaacatgtat agaagaaaca tctcattaaa atcaccagta aagaaatgga aatctttagt
      781 ttttttaata ttatcagtta gtaacttttt ataatcataa aaaaaataag tagttctatc
      841 tgagcgatac tcttttataa tactgaattc ggaattaaaa accacgatag gtaactttaa
      901 acaaacatga agagacttta ataagacttc tttatctgaa aacacttttg tttctctctt
      961 tcctttctac atcattaaat agtattttat acgttatcta aaatactatt cctctgtcaa
     1021 aataagctaa cttacttcat tataaaatat ccacttatcg ctttcatgta tattccaccg
     1081 taaaaagtac attttatcac cgagaactca tcatttttca tcatgatgaa tactatttac
     1141 atatatctag ttcaaagaca ataattatta tgatccgtta ttatctaagc actatacaga
     1201 taataggaat ttttcgtttc taaatactcc aatttgggaa tccaataaaa attttcgttc
     1261 aaatacgtgt ctattcgaac tcttttctag ttgttaatta tgaaggggga agaaataaaa
     1321 aatacaatct atacacgaat aagaatatta tccaaaagga ggttttagaa aagatttatt
     1381 atacgtaaaa agctcaagac attttcttag ccataaggac atgtttgata tttaagaaga
     1441 tatatgaatt ttcttttcta agtataaaga taactatcca ttatcaacta aaaaaacatt
     1501 tttgaaaata aacatcagca gtcctatcaa tgcatgtgag cttatagaag ttattcgcag
     1561 cgcagcataa ttgcattagc ttcttttcgt gttttttgcc tcttttccct ttaaatctta
     1621 atggaggagg tgtgcaatat gtcagataac gacgaacgcg taaatgccca gttgattcga
     1681 ttctttgaaa aggtcatttt gaacaccgct aatacttatt ataaaaaaca agaagaaatt
     1741 caaaaacatg aacaacttga tcaactccca ccatatttgt ttactaaaga tactataaaa
     1801 tttaaacaac cgattaatgt tttaaatgtg actctccttg ttgaagatat aaaattagca
     1861 aaaatccttc ctttgttgaa agaacctgaa caaatttttc ttatcgaaaa atttatcttt
     1921 aataaaactg ataaagaaat tggaaaggtt ctgggtatta caagacaggg tgcgacaaat
     1981 ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg tttaatatct gcataataac
     2041 taattttgta tgcttctttg ctcgtcctcc tccttcccaa aaaaataaat agattataag
     2101 ttcatgggaa gaaggtaaat ttatgaatac tttaaaagaa gatttcattt tagcaaagaa
     2161 tggaaatgaa gaagcagttg tggccattct caaaagattt agcgctttgg tccataagca
     2221 atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc ttgcaatata
     2281 tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc tgtgacataa
     2341 gtaaaaagat agtttcaaat ttcgaacaat aaatttgaag ccatcttctt tataattata
     2401 aagtatcgat tttacagcgt tctataatga ttcatagaat ctaaaacgaa tgattttaat
     2461 tattaacgtt ttttaattcg tttttagctt aaaaataact tttgtcacag tctcactcct
     2521 aaaattactt tatttacgta cgaagtgggc aggaggtcga atccagaact gggccatggt
     2581 acttcaacga ggacttctcc tctagaattg aaaagcatgc gatttacctt ccgtaacgac
     2641 ggtagattta ttcaactatg tgtccaatca aaatttgtct gatttcaaaa atagtcaaga
     2701 gaattttggt tgttaaaaac tcttatctat caacttccac agtttagttg acaaaccctg
     2761 atactaagtt gggctatgat acacttttct tgactatcag ttacgttacc ggaaggaatg
     2821 aactctattg aaaaaaacat atgatcgtaa atttaaacta aagatttcac aggatatcct
     2881 tgagaaaaaa gtaactacca agaaaattgc tgaagaatac aatatctctc gtccaacaat
     2941 ttcccgatgg gtttcagaat atcgtcgata cgggaaaaat gcttttgctg gacaaggaaa
     3001 aagattacct gataaagcag atttctacat tcttgaacag gaaaataaac gactaaaaga
     3061 ggaagacgag tgttctacga acacaaagat cgatacggat ctccagggat cactcaaaaa
     3121 ctttacgatg aaggaatcga aatcaataaa cgtgttgtag ctgttcttat gcgtgaaatg
     3181 aatttatgca caagaggatt tcatcgacgc aaatcttctc atggtagaga aaaaacaatc
     3241 aaagaaatgg taaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt
     3301 tgggtgacgg atattactta cattccatgt aatgacggtc gcctctattt gtccacctat
     3361 attgacttag caactcgtat tctacgttac tatatggtgg attcacacat gaaaaaagag
     3421 attgtcgtcc gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat
     3481 agaggtagcc agcatcggtc ttttatttat caagagctgt tgaccgacca ccacatcaca
     3541 cattcaatga gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga
     3601 tcaatcaaat gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg
     3661 ttaatccaag attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg
     3721 atgactcctt gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg
     3781 acgccttctg ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttgaaa
     3841 tcctttagaa taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag
     3901 agtttaccaa agagttacta gatattctag acctaaatct tacctttcat gaagatgcct
     3961 ttcgcaaaga gcgcattaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc
     4021 aaccagagga atgcttccat tgccactatc aaaataagca aacgatcatt aaatggggct
     4081 ggaaaaaagt atccatctta ttgaatgatg tcagtaacta caagaccatt gtgcggatta
     4141 acaaacagcg cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta
     4201 gcgatcgtcg ctgctccatt gcccgcagag tcaaacaagc cattcttgag ctattaagtg
     4261 agcctatttc tatgtctttg attgcacgta tgaagcacat ttctccgacc actgtcattc
     4321 gaatacttcg cagccttcgg cctaaaaccg tttctttgaa ccagccgtta ccagaagtgg
     4381 tgtgtttcga tgaattcaaa tcggtgaaaa acgtctcggg agccatgagc tttgtcatga
     4441 tggacggcaa gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac
     4501 gcgactattt cttgcgctac cctagaaaag tccgtgaaca agtacgtcta gtggtttcag
     4561 atttctatac accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg
     4621 ccgatcgctt tcatattagc caacacatcg gtcgggcctt taccaaccac cgaattcaag
     4681 taatgaagac ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggagac
     4741 tacttcaaaa gaatgcgtgg gaattgaatg gtcagcatcg ctactggcgt ccgtcatttc
     4801 gagatcatct gacagaagcg gagatagtcg atcggctatt atattatgat gactccttaa
     4861 agcgaggcta cgaagtctac caggtttttc tttccgctat cagaagacaa gatgttcctg
     4921 aattcgtcgc gctcctaaaa gaagactaca aggagttgcc agagcactat caaccagtgt
     4981 tcacgacctt caagaaatat cgaacagaaa tcaaacgagc gttgcgagtt ccctattcca
     5041 acggccctat agaatgtttg aacaaccata tcaaagtact aaaacgaatt gcctatggct
     5101 ttagaaactt tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tatttcaaga
     5161 aaactaagaa cactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag
     5221 ctgtctagcc gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga
     5281 cgaagagcca aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa
     5341 agatacttgt gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta
     5401 ataaagcccc tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta
     5461 tttccagatt agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac
     5521 ttatggggta aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg
     5581 atttgatcag gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata
     5641 ttatattgtc ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg
     5701 aagcgatcca tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt
     5761 gtttcattct ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa
     5821 atgccaataa ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac
     5881 gatatctggc taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta
     5941 tctgcattaa gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc
     6001 atcttcatac caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct
     6061 tccttcaatt cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta
     6121 cccaataatt taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa
     6181 aatcactatg ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat
     6241 ttttatccgt aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa
     6301 aacttgagat accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact
     6361 ctttgaacca gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa
     6421 ttttcgataa gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact
     6481 tagcaattag accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta
     6541 aaagtaatat gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt
     6601 cttcaactac attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat
     6661 cccaaaaacg ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca
     6721 aatcaatgtc taaagggttt ttagctaata taattgcttg aataattgga attggtttgc
     6781 tgattcctga agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta
     6841 gttcaacaat caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa
     6901 gtttttctcc agttctttta aaacctttct tgtcgactaa taccccatca acatttactt
     6961 cccctatgtc atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat
     7021 ttagaagtat cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt
     7081 catagggaac ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag
     7141 tcataatcga tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt
     7201 atgagccagt attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa
     7261 gaaggatttt ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta
     7321 catgaactga gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg
     7381 cttatcgaag aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa
     7441 ttgttcgtca tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga
     7501 agcatgaaca tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg
     7561 atcttttcga tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc
     7621 atcgaggaaa aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta
     7681 ctcattcgat ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga
     7741 aagccgatat tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa
     7801 aatcatgctt ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg
     7861 agaaaatgat tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag
     7921 atagaggaaa agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct
     7981 tttattttcc agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact
     8041 cattcgagaa tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa
     8101 tcagatgatt gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta
     8161 tgaagtcttc tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc
     8221 agatcaaatc caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag
     8281 ctattatgcc gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa
     8341 tcaggatccc gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca
     8401 atatcagcta atgtactccc atttaatctc atttgctttg caaattgata acgccgataa
     8461 gcatcaatat tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa
     8521 agattggatt ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg
     8581 gatagaaagt aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa
     8641 agaaaatatg gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat
     8701 atttaaaacg accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc
     8761 ctttttcata attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac
     8821 cgttgactac aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat
     8881 agccagtagt ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt
     8941 tgtaatcgaa aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt
     9001 cgcctgtata atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag
     9061 caactgtctt ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag
     9121 ttgttttctc taacgctttt agattgtctt ccattactga aacataatct tcaccattat
     9181 ccatttgttc ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt
     9241 cattggccaa agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga
     9301 tcttattttt tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt
     9361 caggacttag tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa
     9421 aggctgcatg ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt
     9481 cagcatccaa ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg
     9541 cttttttcgg atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca
     9601 ttttgggtga aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat
     9661 catgatcttc ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat
     9721 taggcccagc tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt
     9781 tatgatatac aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat
     9841 agtcatgagg ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga
     9901 tgtttttggt gaaatcatac attggataga atgttgttac aatttctaat tttccatctt
     9961 tatcattcga tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg
    10021 ctatcccaat aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa
    10081 tttcttcatt ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt
    10141 gatattcggg cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa
    10201 ttacccctaa tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt
    10261 ttgatccttt cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt
    10321 cgagtggtgt attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct
    10381 tgatcttgca aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc
    10441 agccggtcaa ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga
    10501 attgatttaa actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt
    10561 tctgatgaat cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc
    10621 tttttttgaa tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt
    10681 ttgatttctc tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca
    10741 aaaatgcgat ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca
    10801 aaactgtcac tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga
    10861 ggcgctgtta aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg
    10921 atgatttgtg aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt
    10981 gctaataaat cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt
    11041 tcctcgctat gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt
    11101 ataataattt cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt
    11161 tttccagccc ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt
    11221 taccacttag ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact
    11281 tttttaattc taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt
    11341 ctccagtttc gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac
    11401 ttatcgatct tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga
    11461 ttccaattgt gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat
    11521 acattgaaat aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc
    11581 aaatgataag ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg
    11641 ggtcggtaaa cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca
    11701 tttatctagc gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg
    11761 tgggtaaata atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag
    11821 ctcctttaat tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc
    11881 tataatcacc attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc
    11941 ttcttaaccg tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc
    12001 cttttaccaa cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa
    12061 cttaatacgg gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc
    12121 acgaagcctc agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc
    12181 tcctgcggct tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt
    12241 ctttgcttta gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat
    12301 ttagacatct caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg
    12361 ctcttgtcaa caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa
    12421 cgaatttatc ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct
    12481 gaaaaataag aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt
    12541 ttcctctact taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt
    12601 ttaactggtt tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga
    12661 cattaagtga actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt
    12721 taataaaatt attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat
    12781 ttaaagccta tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag
    12841 tttagttttc tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt
    12901 accttgatca actatttttc cttgatccat cactacaatt ttatttacct tttcaataga
    12961 cttcaagcga tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc
    13021 ctgaatacgt ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat
    13081 tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag
    13141 tgaacttccg ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc
    13201 ttcgcagtta gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc
    13261 aatacgtata ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc
    13321 gattttgcta aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat
    13381 acagccttca tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga
    13441 accactagca cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc
    13501 taaaattggg gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga
    13561 tttcagagga ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg
    13621 tattttagga gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc
    13681 ctttattttt attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt
    13741 taacagtaag tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga
    13801 ggataaggcc atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc
    13861 ttccattttt ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc
    13921 ttgctgcaat tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata
    13981 tttagtattg gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa
    14041 cgttggcaaa ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat
    14101 aaaagggaaa aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc
    14161 aataccttca acgtcagaca tgatagtttg agataaatct gataaattat gttttgaaaa
    14221 ataggataat ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc
    14281 tttataagta ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag
    14341 caaagtaacg aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa
    14401 ttcgtctatc agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa
    14461 gctagaaata gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt
    14521 tttataaaaa cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca
    14581 attgtttata cacttgatgt ttagccatta attcattatg atttccacgt tctactattt
    14641 tcccttctga caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta
    14701 tcataataac tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat
    14761 tatctggatc aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa
    14821 ttgctctggc aatagctata cgttgttttt ctcctcctga cagataaact cctttactac
    14881 caattaaggt atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc
    14941 ttagcgcgtc aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt
    15001 aaatactctg attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg
    15061 tgtcttttga atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat
    15121 tgtagaaacc tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta
    15181 atgcataaat tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt
    15241 tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc
    15301 catattttag ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat
    15361 atcttgcttc aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa
    15421 ttccacttaa aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag
    15481 aagttataaa aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt
    15541 gaaaaagaac aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat
    15601 cgttaattgc tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa
    15661 cttgaattcc tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat
    15721 atttttgaat aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta
    15781 acatggcgat tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata
    15841 aaacaggtgt tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg
    15901 ttttagccgc gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa
    15961 aacgaaaact tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc
    16021 gaaacccaag gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta
    16081 accaagcaac agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta
    16141 gagacttaag aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat
    16201 cagaaaataa agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt
    16261 ttttgtatac tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa
    16321 ctgttaatgc tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact
    16381 acgttctatt ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat
    16441 aaacttgcta caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt
    16501 gcatccattg ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta
    16561 aagaaccata taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac
    16621 gcaacttgag gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc
    16681 gccatactaa tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat
    16741 aacaataaaa caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata
    16801 taaacaaata tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac
    16861 gccccaccaa tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta
    16921 gctaaaccaa taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg
    16981 ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt
    17041 tttttattcc accatttttt aaataaataa ttctattgca tgttttataa agaaattccg
    17101 gatcatgagt ggctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa
    17161 tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag
    17221 tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa
    17281 acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat
    17341 ctttactaac tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt
    17401 ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta
    17461 tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca
    17521 caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt
    17581 ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga
    17641 tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt
    17701 cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt
    17761 gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt
    17821 tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg
    17881 aagacggttc atccaatata attaatttag agtttagcga taaactacat ccaatagaaa
    17941 ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat
    18001 ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt
    18061 tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat
    18121 cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt
    18181 tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat
    18241 tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac
    18301 tacctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg
    18361 aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa
    18421 aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa
    18481 gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca
    18541 gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc
    18601 tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca
    18661 gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt
    18721 aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac
    18781 catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt cccaaaaaat
    18841 atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa
    18901 taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta
    18961 gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga
    19021 aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca
    19081 aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag
    19141 tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt tttttaatta
    19201 actcagtaat tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat
    19261 agtcttttct tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta
    19321 aatgtagaag tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt
    19381 ctttaactta tctagacaac agactgtctt ttttgtcaca aatgatgtaa ttttttcaac
    19441 aatatcttga tatttggttc cagctgattg gaataaaacc aaatcgaata tacgtttatt
    19501 ctcaaaaata taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc
    19561 tatatctata ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat
    19621 aagcgcatta atatgcggct gaataatttc ttcaaatagc gcttccttag aagtaaaatg
    19681 tttataaaaa gcacccgtag ttacgcctgc ggtcaagcac agttgcctta tcttagtttg
    19741 ctgaaatcct actttttcaa aatggcttag tccactttct attaattttt ggtgagttgt
    19801 aatataatct ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctatattatc
    19861 ttacacagga cttgaagttt caatcgttaa cctctattac tgaactcatt ccttagagct
    19921 aaactaatga gaaagagcaa aataactgat acaaaaagca atcctacaag aattaataga
    19981 ctcatctaaa tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc
    20041 cagctattag gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa
    20101 aaataaagcc ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc
    20161 agttaattga aatcctatat ctaaatagtc ctcatatcta agtttagtca gcatctcact
    20221 tacagatgct ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt
    20281 tctttttgat aattcctgaa ttgctagtaa ataaattagt atagtagttg ttttaatttg
    20341 taccatacct accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat
    20401 ttcatcatcg catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa
    20461 atcgttgaat agataggaat attggtatct tttgatactg ttttcattgg tctatcgtcc
    20521 acactgcttt ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt
    20581 tttatttgat ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc
    20641 acattatacg ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta
    20701 tcattcggaa ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca
    20761 atatacttat ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac
    20821 tgtttttcaa tatttttagc gtaaataata ccgttttcta aatttaacca agcatgggga
    20881 tcttccttcc ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct
    20941 gcaaaatagt ctttgttttc ctctttgttc gcatttttta ctaattttgt aaaccaagca
    21001 tttcctccag tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt
    21061 tgaacatctt caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg
    21121 tgtaaatcaa ttttatcttt tgctatattt tcagtaatat ctgctaaaat cgaattagta
    21181 actactacct ttaatttttc gttcgaagcc actgtatctt tgtctttact atttgttgaa
    21241 catgccccga atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat
    21301 tttctcatca taactttctc tccttctttc ttaaaaattt ttgttttggt gagattagaa
    21361 aactaattac aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa
    21421 aactataacc aatgaacaaa cctagcaatg atgagcacgc tccgactgtc gccgaaataa
    21481 atatcatagt ttttaaactg tttgcatata aatatgctgt agcagctggt gtaattaaca
    21541 ttgcaacaat taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata
    21601 gaatcattaa tagataatga taaaaactga cattcatccc cattgctttt gccattaatg
    21661 gatcaaaaga agtgattaat aattctttaa aaaatagaac aactgtaacc aacactaaaa
    21721 cagaaacgcc aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata
    21781 agatatgaaa taaatccgtt gagctatttg caactcctat aaggataact cctaatgcta
    21841 aaaaagaact gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta
    21901 taaaagtaat aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa
    21961 tacctaaaat ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca
    22021 taagagacat tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa
    22081 ctacagatgt aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat
    22141 tttgaagcat attattcccc ttgtatatta gagttagggt tttaaccttc cggttttgat
    22201 tggataaccc tatgaaccat cgttatttta atttggtatt aactcatttc ttttgagata
    22261 atatatttat tggattgaga ggctaagccg ttcccccctc tccaataaat atagtatctc
    22321 cataggcttt ttttaaattc tcttcattaa atacagattc tacacttccg tgaacaatta
    22381 gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat
    22441 gcacaattag cactgttttt ccctgatgtt tcaattcttt taacgtatcc ataattattc
    22501 gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt
    22561 gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt
    22621 gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt
    22681 ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata gatacacatt
    22741 cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct
    22801 tcaccagttt tttattgata tcttcattat taatggagac ctctccctga tgagggataa
    22861 tatttaacat tgctttaagc aatgtagatt ttccagcacc atttggacca ataatacctg
    22921 ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac
    22981 aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca
    23041 cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata atttagattt
    23101 ttttctgttg ttgaagatta ggatgtcaaa attatttgaa actactcgta tagtaaatag
    23161 taagtaattt tcaaaacacg aagaaaaaca aaaagaaaaa aggaaacgcc gaagcatttc
    23221 ctttcagtga tttaatgaga acaacacgat ttgacttaga gtctcaaaaa gtcgaatagg
    23281 tacactcatt taagtgtctc cattcgactt tttggggatg agctggctat tgaggtccgc
    23341 ttaccctatt gaataaatac aagattgggt atttctaata agtccgccct tcatattggg
    23401 gtgatcgggg cgaacatgcc atcttttcat ataccacctc ctcgacactc aataatcaaa
    23461 gattctcgtt aatcttcttc gatgcttgca atgatgtttt caatattcat ataacgaaaa
    23521 tcttcttgtt tgatataaac gatctgagcg gtacattttt cctttttgat gatttctttt
    23581 actttttcct cagaacgtgc gacctgcggc gttaaaagta aaacgtcata gtctttataa
    23641 ttcagttttt cttcaacgtt tcggatcgtt gcataatcta tctcatacga tagatttttg
    23701 gctctgatcg tttcaagcat ttttgatacg agcagcgacg cactgattcc ggtctcgcaa
    23761 ataatcaata gatccttatg cataataccc tcctgttttt agcctgcaaa gccaattggt
    23821 gaaaatgcca cataagttaa aataacaatg actaccaatg ctatagcagt caccttggtg
    23881 tatttccaag gctcgatatc gacctttgta tcgaatgagt tcaacacaaa ttctccatca
    23941 ggtttcaatt tggaaaccac catcaaaatg ataatgtcca gaacgaataa tgccgtcaaa
    24001 acataaagga aatggatatt ttggaaaagc atttttgata gtgcatacaa gacgatatgc
    24061 acggcaatcg tcgtttttgc cccaaaagca gttgcttttt ttgaataaaa ggcaaagaga
    24121 atgatcacga gcagcggcat gttgtatagt ccattgaatt cttgaacgaa ctgatacaat
    24181 ccagccggtg caaaagaaat gaatggcgct acgataatgg agataatccc cacaacgacc
    24241 gttactgttt taccagcacg agcgatttgc ttatcatttg ctttcttatt gataagcggt
    24301 ttataaaaat ccagtgaaaa caaggtcgat gtcgcgttca aagctcccgc aaatgatgat
    24361 aagatcgcgc caaagataac agcggcgaaa ataccaaaga tcattttagg caatacttca
    24421 ctgactaaaa aaggatacgc gttatctgga tttgacatta aactatcgcc aaaaatattg
    24481 cgagcgacga tccctggaaa gacaaggaat aacgcaccta agattttgaa gacgccgact
    24541 aggaaagcgc ccttttgtgc ctcagctaaa ttcttgccgg acaatgcttt ttggacgatc
    24601 atttgattgg tacaccagaa atacagattg ttaaacagca tccctgtaaa tagtgtcggc
    24661 catggaacaa cagccgagtc gatcgatccc acagagttaa gcagccatgg cgtgtttacc
    24721 acaacgtgtt cgatcccgcc aacaaagctg ccgtcaccaa gcttcattaa gcctaaaatt
    24781 gggaccagca gaccacaagc cagcagaccg actccataaa tggagtcact attggcactt
    24841 aacgacaagc cgccgatcag cagatagagg atgccgacaa taccaattgc catcgctaca
    24901 attatgatcg cgacgatcgg ctggacgccc agcagctcat caatatggaa gatcttattg
    24961 aacaccagcg atccagaata aagaaccacg ggtaagaaag aagtcatata ggtcacaata
    25021 aataagatcg aaataattcg tttggtaacc gtatcataac gaatctcgat gaaatctgag
    25081 attgtattga tgccgtattt aaaatatttt ggcaagaata tcacagccaa agctacgatc
    25141 gcaatcgtag cagttacttc ccaggccatt acttccatcc ctgcatgata actttgacca
    25201 ttttgtccga ctatttgttc ggtggacagg ttcgtcatga tgatcgtgcc ggcgattggc
    25261 aaagcagtta agctccgccc tcccatgaag aacccttctg atccagaagt atcgacactt
    25321 ctggatctag aataggcaaa taaccataca cagatgacaa tcactaggaa actgattata
    25381 gaaaaaagat tcatcgtcta gctccttttc aatagattaa agaccggtga cctctcgaat
    25441 gtagcttctg gctttgactg catattcaaa tggattggct ttagccgggt cctgttctgc
    25501 ttcaactacg atccagcctt catagtcgct ggcatggatt gctgtccaga ttggagtgaa
    25561 atcaatcatc ccatcaccag gaaccgtgaa gacgcctttc ttcacgccgt ttaagaagct
    25621 gtctttttcc ttttctactt cttcagccgt ttcttgacga atatctttga aatggatgtg
    25681 tttgatacga tcttgatgac ggttgtaaac cgcgattgga ttttcaccag aaaagaccaa
    25741 atgtcccgta tcgaacaata atttaacttt gtctggatca gtcatttcca ttaaacgatc
    25801 gatttcttcc gtcgtttgaa cgccggtacc catgtgatga tggtaaacga tctccatatc
    25861 cttttcgtgg gccaaatccc ctaatttttc taatccttcc gccagttttt tccactcttc
    25921 atcagtgaaa actggtttat catcaaagac tggcgctgtc ataatccctt ggatactatg
    25981 accctgctca gctactacga tgactttagc gcccatcgca tgtaagaaat cccgatgtgc
    26041 tttgaaggct tcggcagttt cttcaaaagg tttagttgtc agaaacgcac tgaaccatgc
    26101 actggcaacc tttaagccgc gaatattcaa atattgttcc aacacctctg gatcttttgg
    26161 atatttgttg ccaatctctg ttccggtgaa cccagcaagg gccatttcac tgatacattg
    26221 ttcaaaggta ttttcactac caagctcggg catatcatca ttggtccacg cgattggtgc
    26281 gatggctaat tgaatttgtt tggtcatatt ctcacactcc tgcttatttt tttagttgta
    26341 ctaaactctt cgtatcgaat gattcttgac aagccaacgc gatcttagtt gattgcaatc
    26401 catcttctgc attaaccgct ggttgacgtt tttcttgaat acatgagaca aattcttggg
    26461 cttcacgaat gaaggcttct ctgaatcgtt ctgggaagtt ttgtgaacat ggacggatga
    26521 tcccttgatc gttcatgatc gtcacaagat ttttctcagg cgcttgcgct acccgcagca
    26581 ttcctttggt tcccatgatc tctgtttcga catgatagcc atgggctgcg tttctgccgg
    26641 caactagtaa agctattgtt ttatcgctca ggctcatcat tgcggctcca gtttcaagct
    26701 caccagcctg atccaattct ggatacgccg catttttccc aatcgcccac acttgatcga
    26761 cttcttggcc ggtaaaccag cgaaccaaat caatatcatg gatcgccata tctaagaaca
    26821 agccgccgct gtcgttattc atggcaaatt tgacaaagct ttccaagcct tcgctcggat
    26881 cgatcccata acaacgaatc agtgtgattt cgccgatttc gccatcttct accattttct
    26941 tcgcatactg atacgactca tcatagcggc gcatgaagcc taacatgaag acctgttcag
    27001 gatgctcttc gatgactttt tgggtctttt taatatcttc aacttcaagg cctaatggtt
    27061 tttcagtaaa gacatgcttg ccattttcca tcgccaagcg gacttggtca cagtgaaagc
    27121 ctgatggtga aacaataaag actgcatcga tcacggaact tttgaccatt tcttcatagc
    27181 tggaaaaagt atgcttgacg cctaatttct tctgtgcgaa tgctaattct gtttcaacta
    27241 aactgcacgc agccgtaagc tcacaacaac tgacacctgt tgctaaattt tgtgcatgta
    27301 cttttcctaa tcttcctaaa ccgacgatgc ctactctaat gttgttcatg aaatacctcc
    27361 gttttctgag aattactcat tgtatagtgc tggtcgttca tccatttgaa tcattacttt
    27421 tgtttttgtt tcacgagact catgacaagc catcatggtg atcgctgcta catacccatc
    27481 ccaagcggta ggaccgacaa ccgcatcatt tttgatcgaa tcgatccaca gcttgaattc
    27541 ataatcatag gcatccaaaa agcgggtctg ccaatctggt gaaacaggtg tgtaattttt
    27601 ttcttccgat ttaattcgtg tataggctgg gtccgtcatt cctacttctg ccgtttcgaa
    27661 aaccacatca catttaatat cgtagccaaa gcggcaattc acaaagattt ccaaatcaat
    27721 cgttacaccg gattttgttt gaaagatcag cagctgcgga tcatgcaaat cggcatggga
    27781 gttgctggtt tttttcggca agatcatttg aacagattca taatcttcat ttagcaacca
    27841 gcgaagcaca tcaatctcat gaatcgcggt gttgctgatc gccatcggtg ttgtgtagtt
    27901 ttcatctgcg ctaggatttc tatgggcaca atgcatcatt aatgcttctc cgtattgctt
    27961 cgattcgatt gcttctttca gttcaatgta gccgcggtca tagcggcgca tgaagccaac
    28021 ctgaaccaat ttcttcccat aagcaacttc cgcatcaacg atccggcggc agcctgctgc
    28081 atccatcgct aatggctttt cgcagaaaac atatttccca tgcttgattg cttctaacac
    28141 atactgctca tgggtcgggt cccaagaagt cacaacaacg acatccacat cttcagaagc
    28201 gatcaatgct tctccggttt caaagaattt agcatcgaca cgtttggcaa cctctttagc
    28261 agcttttaca ttgatatctg agactgctgt cacataagcc ccttgtgttt tctgattgat
    28321 cctgtcgata tgctcgatgc cgattgcacc tgttccgata acgcctactt ttactctcaa
    28381 ttacttatcc ccctgttctg ttgtttaagc acacatagta tgcgcttaca aaattattcc
    28441 tgtgtagctt ttattcttaa aaggtagagc cctctctttg ctccgccttt tactacccat
    28501 aagcttgtac gtataattcc ttaatttgac tatgactagg caccttttta ttattttgcg
    28561 gacttccgct ggtcaacgca tccgcggcca ttttgtctaa ggctgccata aacgcttgct
    28621 gatcaatacc ataatctcga atcgttggga tcgacagctg ctcacagagc tcttggattt
    28681 ttttaatcag ataatccgca gcctcttcca gcgataaatc ttcggctgcc ggataaataa
    28741 atctggcgat cgtcgctaac tcagtctgaa tagcatcctt tgagaattca aaaaagaccg
    28801 gcaataacat cgcatttgat atcccatgag gaatgtgaaa aagtgccccc accggtcgac
    28861 tcattccatg aatcaatgtg actgacgcat tcgaaaaggc aatccccgcc tccgttgaag
    28921 ctaagctcat ttgttctctg gcttcagtgt cgtcgctatt ttcatatgcc tgagaaagcg
    28981 tgttcaaaat tttcttcatc gcttccagtg aaaatagttg cgttacttca ttggctttga
    29041 ctgaaacata agattccaaa gcgtgacata gcgcatcgac tcccgttgcc gcggttactt
    29101 ttggtggtac agttttcgta aaatcagagt caacgattga gatctgtggc atgaaaacga
    29161 gatccttcat catcattttg actgctgttt ttgtattggt aataactgcc acatcggtta
    29221 cttccgaacc agttccagca gttgttgaca ctgcaatcag cggaatgccc gcttgcagct
    29281 gatcaaaatt tcttgcatga atcgcttcga gacttaattg attagcagcc aaaacggcta
    29341 ccaccttggc tgtatccaga cagctgccgc caccaaaagc aatgatatga tcacattgat
    29401 gcttctgaaa aagcttcagc gcatcataaa cattttcttg cgtcggttcg gcttgaatgt
    29461 ctgtatactc cgctaattga tcatctgttt ttcccaacag ctccttgatc ttttgaagat
    29521 gcccggtctg ttccataacc gtatcactaa tgattaacgg cttgttgcct agttgttctg
    29581 attcttccat aagaaattgg attgttccgc cgccaattct taaattggca ggcattttca
    29641 gttgaatatt cacagaaaaa acctctctct ggcttatagc ctataacaga atcctcgaag
    29701 ctctttaaca gagcttcgaa gcaaatcttt aaatctcact ttgcgcataa cgtgctgtaa
    29761 caactttccg atgggtataa aactcaacac tgtctttgcc attggcatgc aaatcaccaa
    29821 agaaagagtg cttccagcct gaaaaggcaa accaagccat cggtgcaggc accccaagat
    29881 tgacccccag catcccagca tcaacatttt ctctgaagta gcgaatcgct gatgcgttgt
    29941 tggtatacag acacgaacca ttcgcaaatt cagattcatt cgtcagctca acggctgcca
    30001 tcaaatcttt tacccgcacg attgatgcga ccggggcaaa aatctcatcc tgccagatct
    30061 tcatatccat cgtgacatta tcaaaaatcg ttggtccaac aaaatagcct ttttctggaa
    30121 tgttttcgcg accatctagt accagtttcg ctccttcatc gacccctttt tggatatagt
    30181 caaaggtccg ctgctgtgca tcttttcgaa tcactggtcc taaaaagacc ccttcgtcca
    30241 agccattgcc gatcttgatc gcttttgccg cagccttaaa tttttcaaca aactgatccg
    30301 cgatttcctc ttccaaaaca atgacggagg ctgccatgca gcgttcacca gcagaaccaa
    30361 aagctccgcc aataaggtcc ttgactgccc gatcaatatt ggcatctttc agcacaacaa
    30421 catgattttt cgctccagtc aacgcttgta cccgttttag attttttgtt ccttcacggt
    30481 aaacgtattc accgacattt tttgatccta caaagctgat gccttttacc agtggatctt
    30541 gcagcaatgt gttgacaaca tcgtgggcac catagacaac attaaaaaca ccgctaggaa
    30601 agcctgcttt ttcaaccagc tcaacaattt tctccatcaa caatggtgtt ttttctgaag
    30661 gcttcagcac caccgtattt cctgccgcca gagccatcgg gaagatccag aagggcacca
    30721 tcattggaaa attaaacggt gtgatccctc cgattactcc gattgggtaa cgataattcg
    30781 tgatctcaat atcagtcgcc gctgttgata aagaatctcc catcattaaa ttggtgattc
    30841 ccgctgcgtg ctccacattt tcgattcctc gttgaacttc tcccagtgac tcggacaatg
    30901 atttcccatt ttccagcgtg atcaaacgcg ccaattcact tttatgatct gttaaaagct
    30961 gatgcagctt gaacagataa cgtccgcgaa caggaacagg aacttttttc caagtttgaa
    31021 acgcagcatc ggcagcttta actgcttcgg ctgtctctgc tttgctagac aacggtacat
    31081 tcgccagcgc ttcttctgta gccggattca ttacttcttc aaactctgtt gagcgactag
    31141 cgacccaacg accatcaata taatttctta aaaatcttgt cattt
//
LOCUS       DACEDD010000034        31104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEDD010000034 DACEDD010000000
VERSION     DACEDD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.034"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(19..729)
                     /locus_tag="IWO45_001717"
     CDS             complement(19..729)
                     /locus_tag="IWO45_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0333193.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="IWO45_001718"
     CDS             complement(726..2198)
                     /locus_tag="IWO45_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0333194.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="IWO45_001719"
     CDS             2336..2809
                     /locus_tag="IWO45_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0333195.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="IWO45_001720"
     CDS             complement(2962..3513)
                     /locus_tag="IWO45_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0333196.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="IWO45_001721"
     CDS             3618..4148
                     /locus_tag="IWO45_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0333197.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="IWO45_001722"
     CDS             complement(4631..5074)
                     /locus_tag="IWO45_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0333198.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="IWO45_001723"
     CDS             complement(5090..6250)
                     /locus_tag="IWO45_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0333199.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="IWO45_001724"
     CDS             6757..8457
                     /locus_tag="IWO45_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333200.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="IWO45_001725"
     CDS             complement(8559..9392)
                     /locus_tag="IWO45_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0333201.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="IWO45_001726"
     CDS             complement(9696..11861)
                     /locus_tag="IWO45_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0333202.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="IWO45_001727"
     CDS             12121..14415
                     /locus_tag="IWO45_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0333203.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="IWO45_001728"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="IWO45_001728"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0333204.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="IWO45_001729"
     CDS             15066..16073
                     /locus_tag="IWO45_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0333205.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="IWO45_001730"
     CDS             16095..17114
                     /locus_tag="IWO45_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0333206.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWO45_001731"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWO45_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0333207.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="IWO45_001732"
     CDS             complement(18871..20292)
                     /locus_tag="IWO45_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0333208.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWO45_001733"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWO45_001733"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0333209.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="IWO45_001734"
     CDS             complement(22703..23959)
                     /locus_tag="IWO45_001734"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0333210.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVGALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            24539..25840
                     /locus_tag="IWO45_001735"
     CDS             24539..25840
                     /locus_tag="IWO45_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0333211.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="IWO45_001736"
     CDS             complement(25909..26508)
                     /locus_tag="IWO45_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0333212.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="IWO45_001737"
     CDS             26762..27643
                     /locus_tag="IWO45_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333213.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="IWO45_001738"
     CDS             27656..29266
                     /locus_tag="IWO45_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0333214.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="IWO45_001739"
     CDS             complement(29343..30002)
                     /locus_tag="IWO45_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0333215.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="IWO45_001740"
     CDS             complement(30285..30950)
                     /locus_tag="IWO45_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333216.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         9298 a         6346 c         5646 g         9814 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgcccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcatg tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt cgtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttta ttggctctat aatt
//
LOCUS       DACEDD010000035        30557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEDD010000035 DACEDD010000000
VERSION     DACEDD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.035"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWO45_001741"
     CDS             complement(451..1761)
                     /locus_tag="IWO45_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0333217.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWO45_001742"
     CDS             complement(1773..3191)
                     /locus_tag="IWO45_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0333218.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IWO45_001743"
     CDS             complement(3431..4999)
                     /locus_tag="IWO45_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0333219.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IWO45_001744"
     CDS             complement(5003..5413)
                     /locus_tag="IWO45_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0333220.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IWO45_001745"
     CDS             complement(5410..6333)
                     /locus_tag="IWO45_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0333221.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IWO45_001746"
     CDS             complement(6330..7208)
                     /locus_tag="IWO45_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0333222.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IWO45_001747"
     CDS             complement(7195..8532)
                     /locus_tag="IWO45_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0333223.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IWO45_001748"
     CDS             complement(8535..9638)
                     /locus_tag="IWO45_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0333224.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IWO45_001749"
     CDS             complement(9712..11220)
                     /locus_tag="IWO45_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0333225.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IWO45_001750"
     CDS             complement(11186..11878)
                     /locus_tag="IWO45_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0333226.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IWO45_001751"
     CDS             complement(11900..12874)
                     /locus_tag="IWO45_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0333227.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IWO45_001752"
     CDS             13174..14046
                     /locus_tag="IWO45_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0333228.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IWO45_001753"
     CDS             complement(14504..16702)
                     /locus_tag="IWO45_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0333229.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IWO45_001754"
     CDS             16934..17926
                     /locus_tag="IWO45_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0333230.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IWO45_001755"
     CDS             17986..19407
                     /locus_tag="IWO45_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0333231.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IWO45_001756"
     CDS             19400..20899
                     /locus_tag="IWO45_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0333232.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IWO45_001757"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IWO45_001757"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0333233.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IWO45_001758"
     CDS             22491..23174
                     /locus_tag="IWO45_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0333234.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IWO45_001759"
     CDS             23176..24012
                     /locus_tag="IWO45_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0333235.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IWO45_001760"
     CDS             24023..25777
                     /locus_tag="IWO45_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333236.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IWO45_001761"
     CDS             complement(26053..26394)
                     /locus_tag="IWO45_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0333237.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IWO45_001762"
     CDS             complement(26462..28633)
                     /locus_tag="IWO45_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0333238.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IWO45_001763"
     CDS             28844..29701
                     /locus_tag="IWO45_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0333239.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..>30557)
                     /locus_tag="IWO45_001764"
     CDS             complement(29772..>30557)
                     /locus_tag="IWO45_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0333240.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9123 a         6291 c         5401 g         9742 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaattt
//
LOCUS       DACEDD010000036        30090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEDD010000036 DACEDD010000000
VERSION     DACEDD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.036"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            259..597
                     /locus_tag="IWO45_001765"
     CDS             259..597
                     /locus_tag="IWO45_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333241.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(802..1041)
                     /locus_tag="IWO45_001766"
     CDS             complement(802..1041)
                     /locus_tag="IWO45_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0333242.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1017..1364)
                     /locus_tag="IWO45_001767"
     CDS             complement(1017..1364)
                     /locus_tag="IWO45_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0333243.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2096..2677)
                     /locus_tag="IWO45_001768"
     CDS             complement(2096..2677)
                     /locus_tag="IWO45_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0333244.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2860..3486)
                     /locus_tag="IWO45_001769"
     CDS             complement(2860..3486)
                     /locus_tag="IWO45_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0333245.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3508..4839)
                     /locus_tag="IWO45_001770"
     CDS             complement(3508..4839)
                     /locus_tag="IWO45_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0333246.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4994..5395)
                     /locus_tag="IWO45_001771"
     CDS             complement(4994..5395)
                     /locus_tag="IWO45_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0333247.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5481..5687)
                     /locus_tag="IWO45_001772"
     CDS             complement(5481..5687)
                     /locus_tag="IWO45_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333248.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5782..7284)
                     /locus_tag="IWO45_001773"
     CDS             complement(5782..7284)
                     /locus_tag="IWO45_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0333249.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7302..7757)
                     /gene="argR"
                     /locus_tag="IWO45_001774"
     CDS             complement(7302..7757)
                     /gene="argR"
                     /locus_tag="IWO45_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0333250.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7855..8472)
                     /locus_tag="IWO45_001775"
     CDS             complement(7855..8472)
                     /locus_tag="IWO45_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333251.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8693..10066
                     /locus_tag="IWO45_001776"
     CDS             8693..10066
                     /locus_tag="IWO45_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0333252.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10141..11253
                     /locus_tag="IWO45_001777"
     CDS             10141..11253
                     /locus_tag="IWO45_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0333253.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11325..12962)
                     /locus_tag="IWO45_001778"
     CDS             complement(11325..12962)
                     /locus_tag="IWO45_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0333254.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13130..13738)
                     /locus_tag="IWO45_001779"
     CDS             complement(13130..13738)
                     /locus_tag="IWO45_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0333255.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14122..15273)
                     /gene="queG"
                     /locus_tag="IWO45_001780"
     CDS             complement(14122..15273)
                     /gene="queG"
                     /locus_tag="IWO45_001780"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0333256.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15292..16044)
                     /locus_tag="IWO45_001781"
     CDS             complement(15292..16044)
                     /locus_tag="IWO45_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333257.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16185..17135)
                     /locus_tag="IWO45_001782"
     CDS             complement(16185..17135)
                     /locus_tag="IWO45_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0333258.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17136..17975)
                     /locus_tag="IWO45_001783"
     CDS             complement(17136..17975)
                     /locus_tag="IWO45_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0333259.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17972..18718)
                     /locus_tag="IWO45_001784"
     CDS             complement(17972..18718)
                     /locus_tag="IWO45_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333260.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18956..20326
                     /locus_tag="IWO45_001785"
     CDS             18956..20326
                     /locus_tag="IWO45_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0333261.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20381..21277)
                     /locus_tag="IWO45_001786"
     CDS             complement(20381..21277)
                     /locus_tag="IWO45_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0333262.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21558..22235)
                     /locus_tag="IWO45_001787"
     CDS             complement(21558..22235)
                     /locus_tag="IWO45_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0333263.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22326..23231)
                     /locus_tag="IWO45_001788"
     CDS             complement(22326..23231)
                     /locus_tag="IWO45_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0333264.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23406..24830
                     /locus_tag="IWO45_001789"
     CDS             23406..24830
                     /locus_tag="IWO45_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0333265.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            24876..25907
                     /locus_tag="IWO45_001790"
     CDS             24876..25907
                     /locus_tag="IWO45_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0333266.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25914..27101
                     /locus_tag="IWO45_001791"
     CDS             25914..27101
                     /locus_tag="IWO45_001791"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0333267.1"
                     /translation="MEIKRSLNTKFAEIFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27257..28639
                     /locus_tag="IWO45_001792"
     CDS             27257..28639
                     /locus_tag="IWO45_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0333268.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28611..28997
                     /locus_tag="IWO45_001793"
     CDS             28611..28997
                     /locus_tag="IWO45_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333269.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29197..29865)
                     /locus_tag="IWO45_001794"
     CDS             complement(29197..29865)
                     /locus_tag="IWO45_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0333270.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8445 a         6154 c         5489 g        10002 t
ORIGIN      
        1 tatataacac taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt
       61 taaagtatat cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta
      121 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
      181 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      241 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      301 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      361 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      421 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      481 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      541 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      601 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      661 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      721 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      781 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      841 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      901 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      961 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
     1021 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat tgggaaaaca
     1081 caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt cttctactgt ctctgcacgg
     1141 gcattaatca tcaattgtcc ttttttaaat ccggtaaatc cccacttcat gatttccgga
     1201 gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa gatttgacgg aaaaacttct
     1261 ccagctgcta attctgctgc ctgcttcggt tgtttcgtct gggcttctcg ataataattc
     1321 tgaaggagct ctgactctag atcaaaaaaa taacgaccac acatcctaat ccctcccaat
     1381 ctttttttct atcatacact gcaacaagaa gttcgcacca acaaaacgct ttcaattttt
     1441 gtagaactaa aaaagttgtg acaagtgaca aactctccag tgaatggtat tctcctgcca
     1501 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgctttt tgaaattgtt
     1561 ccttgtgact cggagtgaaa cggcaggctt acgacctctg ggtaaacggt gttcaaaagc
     1621 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagctattt tcacaaaatt
     1681 tttcttaata ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa
     1741 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagcagct atttttattt
     1801 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctccact aaacggtgtt
     1861 caatcagcta accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
     1921 gtttgtttct ctaatactca ggtcaaagcg ctgatttcac aacctctaat acacggtgtt
     1981 ccagaagtag cttcttcgaa aaataagccg aaatttcaca aaaatttgag aagcaatttt
     2041 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct aattattaaa
     2101 gaaccttttg cgaagttaac aatctttctt tgatcaattg aaaattctgc ctaagtagtg
     2161 atagatcctg tgtgactttc aacaataaga tacttccctg gatttcagca atcaacgctt
     2221 tggcttgacg gtctgcttct tcatcggaca gtttttcttc agaaagtgcc aggatatttt
     2281 gtttcgtata cttgacccat tgattgacta attctagtag caacagacgg tattcttcat
     2341 ccactgtact caactcaata atcaaattac ctacaggaca gccatgataa gtgatctgtg
     2401 tctcatgatc agcgaaaatc cagtccaaca tttcttctaa tgcttcttct ggctgttttc
     2461 cagcatttaa gatgtcttcg aacaattctg tctgccatac gtccatatgc gcatggatca
     2521 ccgctagaca aacttctttt tttgaagaaa agtaataata aaattgacct tttcctgtat
     2581 cagaagctgc tagaatatct cggattgacg tttcatgata gcctttttta taaataagat
     2641 ctttggtcgc ttccaagatc ttttcctttg ttttcaatga tattctcctt tttcatttct
     2701 catagtccta tattatacag taaattcatc attttttctt cttaatcgtt taaaaaagac
     2761 tgtgccaaat tatctgacac agccctcact ataaaactgt tctcatatag ctagatgttc
     2821 attgattcaa gaactgcgag gacaaaagca attcctatct tatgatagtt gctctaatac
     2881 ttcctctgtt gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat
     2941 tgcttcttgg ccaggagcac caattgcatc tgtcgctacc acaacattga atccactctc
     3001 taccaattca cgcatatggg aatcgctaca caagtttccg ttcattccac caagaattac
     3061 tgtgtcgatt ccattttttc tcaattggag cgccaaatca ttgctttctg gtccaaagat
     3121 tttatgagga gagctgatga tcgtattttc atccaaataa ggtttcaatt cttcgataaa
     3181 atcggctcct gaaccttctt ccactgattc atattgactt tttctagcaa acatctgctg
     3241 ctcaagcatc atatgttctc ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa
     3301 ataatgagga gaaataaaga gtttgtatcc tttttctttt gcttgtttta ccatttcaat
     3361 catattttcg atcgtattgt atttttctaa gatatctttt gtcaaaccaa agccaccgcc
     3421 tgctggtttc aagaactcga cttgcgggtc agtgagaact actgctgtat ttttattatt
     3481 gaacatggtg gattctccat tcgttattta ttttaataca tcatttagag cttcactcat
     3541 tgtcggatga gaatagattt gatctctcaa taacgtataa ggaagttttc cattcatagc
     3601 tagcgaaatc aagttgatga cttcatgaga atcttccgca tagatacttg cccctagaat
     3661 caagtcagtt tcagggtcga ctaaaacttt gaatacacct ttaggatctt ctaatacttg
     3721 tgctttaggg acaccgcttg tcatatactt gaataatttg taattgacgc cttgtgcttt
     3781 ggcttgtttt tctgttaaac ctacattgga taaagtcgga gtgataaata cactgtaagg
     3841 cacagctttc cggttattcg ttgttcgttt attctcgccc ttcaactgat ccataatgat
     3901 tcgataatca tctagagaga tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc
     3961 ccaaatgttg tctgcatctg tacgaaggta ttcatccaca gcgattgctc cgcgatctgt
     4021 tagtttaaca tctgtatttt ctaatccaag tgatgctgta cttggttttc ttccagttgc
     4081 agccaaaact tttgacgctt ggattgttac ttcttggcca tcttctgtaa aggtgacggc
     4141 agctgttgat tcttggtctg ctacttgatc tacagataca cctagatgga attcgattcc
     4201 cgcatttgtc atgtcttcta aaatcatttg cgcgatatct tcatcttcac gtggaagaaa
     4261 ttcagaatgg gcatcaagaa cgacaacttt agatccgtat tcattgaaca ttgatgcaaa
     4321 ttccaagcca atatatccag ccccgatgat cactaatttt tccggtaatt gttcctgatc
     4381 catcgcttgc gtagaatcaa tcaagtagcg acttgtattc aatcctttga ttggtaaaat
     4441 gattggttca gaaccagtat tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc
     4501 attattcgtc acttcgattt gatggttaga aagaaaacgt gctgtaccgt ccatcactgt
     4561 cccagttgcc tcatcgctga tcatatgata atttttggcg cgaagcatgc ccgtcaattt
     4621 ttctttccga gcaacagcct ctgtaaagtc gattcctctt tctccattga aaatcaatga
     4681 tttagaagga atacagccga tgttgataca tgttccgcca tacatacgtg ttgacttttc
     4741 aacgactaaa accgattcac cttttgaagc taacgccttc gctaaagtct taccgccctt
     4801 tccaaaaccg atcacaatat tttgatattt ttccatcatt tttccctcgc ttctttttta
     4861 cctaccgatt ggtacaaaaa tatgataacc gataattgtc tattctccaa atcatctgct
     4921 ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca cgtttttagt ctggttcgat
     4981 ttcctttatt cttctaaaca atcgttactt ctacatcgat atttcctcta gttgctttgg
     5041 agtaagggca gaattcatgc gcatcttctg caattttttg ggcttcttct tcgctgatgc
     5101 catcgatatg cacttctaat actacaccga tctgatagcc gccatcatct aaattataaa
     5161 gagagacacg tgcttttact gtggactctg ctgtcattcc tgcttctttg atcatatgtt
     5221 gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa taattgttct ggatttgttc
     5281 cttctgcatg gattcctgga ggagtgattt tcatatgcat cgagccattt ggtgcctcga
     5341 cttctccatt gcgcccgcca tggttgatca ttgctgtttc gtatgctttt ttcattgtac
     5401 tgctcctttc gattgaccat ttgctacttg gctataatta tagtgtatga caagtaaggc
     5461 gctttctaca aaaattctgc ttattcgaat accagaatgc cgtcttctac tatttttcca
     5521 aaaaacaaaa aaatactact cgtgaacaac agcgtcatat ttgttccaaa ataaatagca
     5581 atcttttcta cataatcttt tatatgattg attttaagat acatatatac cagaaagttg
     5641 accccataaa ccaatactgt taaaaaaata accatattcg ttaccatttt ttcctccttt
     5701 tgctggtctc cctaccagca ctcgatttaa ccataccact ttttttgccg tccaagcaac
     5761 tagcctttca aaaagactgg tttattctgc tgttaatgat tcaagatcag aaaaatgtcc
     5821 gaataatttt gctaatttta ctaacgccaa cttgttcttt ccgaattcat cattttctat
     5881 ctgtaatact agtaacggct catgtaagct taaacggatc aaaaaccagc cgtttccgta
     5941 tggctcttct agatcatatc gaataccttc ttcattttcc gggtcgaagt tcatcccttt
     6001 ttcttcttcc aacagtcgct tcatttcttc taatatcttg tttccttgtt cgcgtggttc
     6061 atcgctgaac aattttaatc ggatttcttg tacttctacg ggctgtttca actctttgat
     6121 ttgttcatct aatgtgatac cttttctttt taattctggc aaaagcatca ggatctttgc
     6181 gatgacatat gcaccatcat ccagaaaata gttttccttg aatgcagcgt gtccgcttgt
     6241 ttcgatggct agctgtgtat ctttcccaag acggttaagt tctaatgctt tattgattac
     6301 atttcgatag ccggaaatat aacgaacttg gatgcctcct ttttcttcaa tgaatctttg
     6361 tagatgagaa gaagtagggg aattcgtcac gattcttgtt cctggatgct cttttaatac
     6421 gattgtactt aatactgcga tcaaattatt tcgattgatc actgttccag aagcactgac
     6481 aactgcagaa cgatccacat ctgtatcgaa aatgatccca agatctgctt catttgctaa
     6541 aacagcctgc ttgatacttt ccatcgcttc tttattatca ggatttggga tatgattagg
     6601 aaatcggcca tctggttcca aaaattggga acctgttgaa tccgcaccta agctattcaa
     6661 tactttttca gcaaagaatc caccagcacc atttccggca tcaacaatga ttttccaacc
     6721 atttagtggt ttgctttcta gtgttccaat ccccttttta atttttgtga ctaaatcttg
     6781 cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca gtttgttcgc tttttatttt
     6841 ttgctgctgt gataggataa aagcaatatc ttctttttct gctcctcctt ctgaggtaaa
     6901 gaattttatt ccattataat aatagggaag atggctagca gtcagcataa ccgcagcatc
     6961 gcacgagaac tgttcaaatt gagtggacat aaacatagca ggcgtagtag ctaattcgaa
     7021 atcgatgaca tgtatccctt gttctttcag actttggatc aacgcctctt tcagtgctgg
     7081 tccgcttatc ctactgtcat gaccaatccc aatcgtccat tttttatttg gatcggttga
     7141 tttcttcttt ttctttatcc agtttgctag tccccagccg atctttttta ccatgtctgt
     7201 cgtcaagttt acttcatatc cttcaactgg taatgcgatt cctcgaatat ccgatccatt
     7261 ttgcagcgct ttcatatctt tcatataaaa gtcctcctca tttattccct cattttttcc
     7321 agcttttctg caaatcgctg tgcttcttct tctgagcggc aaataataat gaatgtatct
     7381 acaccggcca tcgttccgac aacttcaggg taagccactt catctagcca attcgttacc
     7441 acatcggcac catttcgttc agtcaatacg ataaccatga actgaacaga ctggatacgc
     7501 aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg
     7561 ttaaataatg tatagcgtac tattttgttt tcattttgac tttttactaa cttcatttct
     7621 cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca
     7681 agcagctctt cctgtgtccc aataggttgt tctgtaatga tctggcggat cagtgcttgt
     7741 cgctgtttct ttttcatatt agacacctct caatgttgat tatagcaaaa gataagtaat
     7801 atttcttcat attttggtat tttttacttt tattttttgt tttcattcag gttgctaacc
     7861 tttttctatt ttaacacgca aaatcgtctt cattttttca ggtgctgatt ttcttggatc
     7921 gctgatgtaa atctctttgt gttctttaga agctctttca tagccttctt ttgcgatgaa
     7981 tgcttctaat ttctcaaacg ttgctggttc ttcggcatag ggcccgacgt gtaagatttg
     8041 ggcacaaaga ccttcttcca tctttttgaa tactacttgt tctttcaagt cttccggaag
     8101 tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg
     8161 gatcatcaat tgataagaaa aatgttcttt tttcatcact ggttcatgca aatacttttc
     8221 ttgcagtccc cattgtcctt ccagtggata gacagtatac atctgataat caggaatcag
     8281 ccaatttttt ttaggcgaca tgcgtattgc atagctgact gcatatagac aggctactct
     8341 tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg atgctaaaga atgatttttt
     8401 aggaacctct agtatttgcg ggcttttttt catactatag ctcttgtctt tacgccattc
     8461 aattttttcc atttttcttc ctccgcgaaa ttccatttag ttatttatta tcatacctct
     8521 ttttgagtga cttggctaat cgtgttgaca ctatttttca gtttgtgcta gaataagtga
     8581 agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga gagagaggcg gtttctattt
     8641 ttataataga aatcactctc tttttttacg aaatttttta gaggtgatgt aaatgacaaa
     8701 acaaaaacag ctgcttttag tacctgcagt aattgcaaca gggatgatgt cttttgcggg
     8761 cgtattgatc gagacggcca tgaatgtaac tttcccaact cttatcagac agtttgattt
     8821 aacgactgca caagtccaat gggtaacaac gatttatctt ttgatgattt cgatcattgt
     8881 tccgctgtct acttatttga atcgcaattt tagtctcaaa aaactctttt tagcttcaaa
     8941 tctgcttttc ttggcaggta taatgatcga tttcttctct cctaccttca tggtcctatt
     9001 gtttggtcga ttgctccaag gtgcagcgac aggtatcgct cttccgttga tgttccatat
     9061 tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac
     9121 aacggccatt gctccagcaa ttggccccac ttatggaggc attatgactt cacattttac
     9181 atggaatcat atcttcctgt ttcttgtgcc cgttttgatt ctttctttga ttatcggctg
     9241 gtttgctatt cccgagatgg cagtacaaaa aagtggttat ttggattgga taagcgtttt
     9301 aggaatttgt cttttgttta gtggaagtct aacatttttg agtacgttgg gaacagtttc
     9361 cagctggatt gctttaatca tcggcggtat tggctggttc cttttttact atcgaagtaa
     9421 aaaagtggag catccactga tccgactgtc tattctttca aataaaacat tccgcttgtt
     9481 tttgattggc ttcttagttt atcagttttt attacttggt gtatcttttg tcttgccgaa
     9541 tttccttcag atcgttcaag gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg
     9601 cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa atgctggatc aatatggtcc
     9661 gaagaagcca atttttgctg ggctgattct ttctttgatt ggttggctag cattattcct
     9721 actcatagga cacgcttcag tgggtgtatt gattgcttgt catgtgtttt atatgatcgg
     9781 tatcggatta tcttacagca atatgatgac caccggaatg aatgcactga atgaagaact
     9841 acaaggagac gggaacgcaa tgttcaatac cttgcagcag ttttctggcg ctgtggctac
     9901 ttcattagta gcggtcatta tcaattacgt ccagcatcat acttcccata actatgaagt
     9961 gtctactaca ttaggatcaa aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag
    10021 ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa gcgtagattc catatacggc
    10081 aggtgttgca tctgccgttt ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt
    10141 atggaaaaaa ttgccattat cggtggcggg atcattggta tgaccctagc cagttactta
    10201 gatacatcaa aattcaatgt tgctttattt gatgaaggga caggacaggc aactaaagct
    10261 agcgctggga ttatctcccc ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg
    10321 gctaaagacg gcgctgcttt tttccctaaa cttataaaag atttccaact tgatcaaacg
    10381 atttacgcac aatcagggac acttttgctt cgacctgaaa aagatttagc tgaattggct
    10441 gttcttgccg agaaacgaaa agaagaagcg ccagaaattg gaaacatcga atggctttca
    10501 cctgaacaga catctcaagc acttccgtta ttgaaaccag cagtttcact gcgtatcagt
    10561 ggtggtggtc gtctggatgg aaaagcatat ttagaacatc ttggtcagcg tttagcacaa
    10621 aaacaaatgg agcagatcca ggcaaaagtc actttgcata agaagagcga ttcttggctc
    10681 atcaagggac cgtttggaga atacacagct gatcgtgtaa ttttgacacc tggcccggca
    10741 cttaagcctt tattacaaga attaggctat gctgcagata tacgtccgca aaaagggcag
    10801 ctcttagcat ttcagacatc ttataagatg agtcaagact ggcctgttgc tatgcttgac
    10861 ggagaagctg atttcattcc tttcatggat ggaaagatcc ttttaggtgc tacccatgaa
    10921 aacgatcaag gctgggattt ggctcctact agcgaagctt ataagggatt gactaagagt
    10981 acagaacagt tcttgactga tccagatcag ataaaaaact ggccatacga gttccgtgta
    11041 ggtacccgtg cttatacgtc agatttttcc ccatttttcg gcgtactgcc agatgatcca
    11101 agcttactgg ctgctagcgg tctaggctct tctggcttga ccacaggtcc ttatatcggc
    11161 tatctattag ccaacttctt aaatactggt cattggccta cggaagatta tcaaaaggac
    11221 atctccacct atattacgaa aaatccatca tagaaaaaca gctttttcaa gtaaggatcc
    11281 cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt tattttagtt caatttctga
    11341 atcaaattct ccaaactttc ttttgcttca tctggcaaga gatcttttcc tatctgagct
    11401 tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca gatttttata ggacgtatcc
    11461 tcaaaagaaa caggaaaatc tggtataggt aaatagtgga tgtcttcaaa tggaccaaac
    11521 gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt
    11581 ttgatttttt tatcatcgaa atatccagta ttcaggacat agcaggctgt ttgctgtttc
    11641 aaaaacaact ctcgaaaagc ttgatagtct tccctaagag gatagacacg gaaaggatta
    11701 gcaaatggtt caatgactag ctcatctagc tgtctttttg tagtgatatt ttccgcgtct
    11761 gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta aaactgggtc attgatcttg
    11821 ataattggcg gaagcgaatc atctttcatg atccaaaaaa cagcatcgat cttttcgcta
    11881 agatgatcga ctcggtcggg tgtcgcataa cgtgatttga ctgtccgccc atttccgttt
    11941 cgtatatctc ctgttactag gactttttct cctttacttg tacatgtcac gcccacattc
    12001 tgacaagtaa ggaaagaaga aatggccgga tcatcagacg gatagtcttg tgttttatca
    12061 aagtaagaag gttccaaggc agtagcaaaa ccattttttc gagaaataac gaaagcatcg
    12121 tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt
    12181 ccagaaccgg acaacccaaa tacagccatc gtataggtct ggtcattact agcatattgt
    12241 ttcatcccac cgtggcaggc aataaagcca ttgcgatttc ccaatgccca agcaagcgtc
    12301 aaagtcgctt tttttaattc tccaaagtaa cgtaatccta gcacagccgc tgtattatgg
    12361 agagcatcaa acaaaatgag tccatttgga aatgtttcat gtgtccattc gggatcacaa
    12421 aagatataaa gatctccctc atcaaaggaa acagactttt gataagtctc taagatcttc
    12481 gaattcgctg gctgaaaatt cagcaaataa gaatacaaat tgttctcaaa tccttctgga
    12541 atcagcaaat gactttgcac agaaaattct tcagataggc caacgattac ttttgtatgg
    12601 taaaactggc gcttgcgact ttgatacaca gcttctctta aaattttctc gtatttgctt
    12661 tgttccttgt ctaagtcctc aaagatccgc cgagcacttg ctgttcttcc cacaatgctt
    12721 ccatcattga atactagtgt cctaacatca gctggtaaat ctagttcttc agcatgtgag
    12781 attgttaaat ctgtgatgat cgttcctgga ctcccttttg ctagttgata agctgtcttc
    12841 ctatcaaaaa cttcatgaac attattaccg taaaatgtcg tttctacaat cgatcgaata
    12901 ctggataaca gtggatttcc acttgtgatt tcgtttaatt cgagactctc ttttgtactc
    12961 atggctcctc ctcattccct aaacaaaaaa acgttttctt tattatacat tattcgagtt
    13021 tttatttgta ctgctgcgct gtgggaagca aacgagttat ttttcacaca aaaaaaccga
    13081 gaacttgaat aaacaagttt ctcggtttca tcattttcct tttatcggct tagtaccagc
    13141 cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc ataacgagat gttacatatt
    13201 gttctgctac acgttcttga ttggcagctg aatagtcgcc gtttaagtat gaagcatcta
    13261 attgataacg gccgatgtaa cgtccgttag tagctgtgta tgaaccgcct gattcttttt
    13321 gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt tgcagcagct ggggcagcag
    13381 gagctacttc aactgtttca acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag
    13441 ttgtagatgc tgtattttct gttgttttgt tttctgttgt tgctgaagaa tcatttgaag
    13501 ttgggattgt taattgttca ccaacataaa tgcggttgat gtttgcgatc gaattttttt
    13561 cagcaattgc atcgatcatt gagttgtctc ctgcaaattt ttgtgagatc ttagataatg
    13621 aatcacctga ttttactgta taaacttcat cagcgtgagc atttgtagtt cctacaaaca
    13681 ttgcagtagc accagcagca aaagttgtac ctaaaacaat tttcttaaat gaattcatat
    13741 taaactcctt tttttctccg ttttttattc tttcgacgct attaacttta tcatttagaa
    13801 atatttcata ggtaaaagaa acatgacatt taatgacaaa ataacgaatt atttaatatt
    13861 tgtaacattt aaacaaatat ttttttgata caaaaccata aaagccttgt tgtataagca
    13921 aagtaatcgt ttttctatgt ttttttattt ttgaatgaaa aacgcagatt ctgtaacatt
    13981 cactgcaata atccataaaa aaaagcaaaa aaatttttga tttttatcaa attctcttaa
    14041 aaaaatgtaa aaaatacaaa aaaaccagta attgttacaa caattactgg acaaaaactt
    14101 tgaataatgt taactatttt tttattttaa ctttctccgc aataatttct gggcatgttc
    14161 caattcgttt ataacgactt cttctgtttc tttagctaat atttcatcta atacatcaag
    14221 ccatttttcc ggttcttttg ttcctagacg tccaatagcc caagctgctg ttcctctgat
    14281 gacagggcgg acatctgtta aacattctga aatctcaggc aatgccgttc gatcacctag
    14341 attagctaaa gcaatcaatg cgtttctctg caaaggtttc ttcccacgcc atgaaccagc
    14401 taaatgccca aattgctgtt tgaagtcttt attagacatc gaaagcattg gctttaattt
    14461 tggatatatt tcgtctactt ttggttccat ctcttcatga aaatgaaaat cttttccttg
    14521 attgtaaggg cagactaact ggcagatatc acatccataa atgacatttc gcattttttt
    14581 acgatattct tccggcatca tcccttttgt ctgagtttga taagacaaac atcgctgggc
    14641 attcattctt ccgtcaccaa gcaatgcttt tgtcggacaa gcagtcacgc agcgcataca
    14701 gtcaccgcaa ccaaattctc ctggtttatc aggctcaaat acaatattag taacgatctc
    14761 accaagatag acaaatgaac cgaactcttc agtgatcaac aatccgtttc gtccaataaa
    14821 tcctaaacca gctcgctgtg ctacagcgac atcgatcagt tctccagtat ctacttgcgg
    14881 agctagacgc cattcctctt ctttttgcca tttttctgct cttgtttgga tgaattcgat
    14941 caatcggttc aaacggtctc tgagtatatc atggtaatcg atcccccaag aagcacgagc
    15001 aaactgcccg cgtttttcat ccctaggcac ttcttcatgg atcctagtag gatacgcaag
    15061 tgcaatggaa atgatcgatt tcggctgatc aaatgtcatt tctggataaa gacgctcatc
    15121 gatatttgga tgttcaaagc cagaagtatg gccagctgct ttttgttctt ccaacgattc
    15181 ttttaatgaa tcaaaaggtt ccgccgtggt gaacccgatt ttgtcaattc ctaatcgttt
    15241 gctttcttgt atgatttctt cttttaatgt catggtcttc ctcgcttgct gttaacggct
    15301 gtataaccca taatatagtt tttctttttt cagctggaca aactggatct gctgatcggc
    15361 aatcttcttt tcttccatcc atttttttct tgcagcaaat acttcccaat aatttgttga
    15421 aattaatttt cttttgttat ctaatgcatt aaaaaaattc aatgaaactg gttttgtact
    15481 taacagttca tggagcctta cctcttccca tggcgaaaac tcagacagat cgcgggaata
    15541 caatacttct tcatagagat ttttttgctg tgtgtacgtg aatgtttgcg ggattttccc
    15601 aggcctagca atttctacta gacgattata aaacatattc attaatttgc tgttggcaat
    15661 aaattgactt ttataatagg ccgactggat ctttttgatt actccttctg ctttgatgac
    15721 tgaaaataat gcaggccatg ctttgacggt tatttcgtcg ttttgtttaa ctaatacgcc
    15781 aggtgtgaag ccgactggag aaaggaacac aaaataaccg ataccggtat tttcatctaa
    15841 aggctgaaat tcatcaaaca accatttgcc tatgtacagc tcttcatctg gttctgttgt
    15901 ctcattttga tttgctatga ataatttagc taattctgct tcaagaaatt gaaggtcttg
    15961 atccttgtcg gtattttcct gctggctgat caatcgaaag acttcttctt taatcgtata
    16021 tttatcttta cgtgtaaaat tcatagccac accttccttc cttactctaa ctataacgga
    16081 atttcttcaa aaaaggtatc tgtacgacga aaaacagcat aatttttttt catatctctt
    16141 ataaaaaatg caaggtctgc tcctcaagct agaccttgca cttttcagct ttcttttgtt
    16201 tgtgacaaac cttcatggat cgtttcaata ttccatttca tcatctgata gtaagaatct
    16261 ccttgttctc cttcttttgc tatggaatca gtgaacagtt tatcatagat tttcaaaccc
    16321 gtttcttttg ctacccgttc catgcttctt gagtctacac tggtttccac gaataaaacc
    16381 ggtacttctt ttgctcttat ctgatcaatg atcgctttca tttgatcagg cgtaccttga
    16441 ctttctgtat tgatctccca tatataagct gctggcaaat catatgctcg tgaaaaatac
    16501 ttgaatgccc cttcacttgt tactaataat tttttgttct cttcgataga agcaaaactt
    16561 tccttcgctt gtgtatctaa ttcttttagt ttttccacat atgcttctgc atttttttta
    16621 tagagtgtcg cattttctgc atctttttca gagaatatac gtgcgatttc ctccacatat
    16681 tttattccgt tagataggtc tagccaagca tggggatctg cttttgtatg ttcttcaccg
    16741 ctagttaaat aaagaggttc tacatttttg ctaactgcaa aataatcttt tccttcttct
    16801 tttttagccg tttccatcaa gttatcgaac cagctgttac ctgtttcaag attcaaaccg
    16861 ttgtataaaa taacatctgc atcgcttgcc tttttgatgt cttctggtag tacttcatat
    16921 tcatgcggat ctgttccgac tggtacgata ctgtggatat ctatttgatc tgttccgacc
    16981 tcttttgcca tgtccgccaa gatcgaattg gtagccacga ctgacaactt ttctttactt
    17041 ccttcatccg cttggtcaga agcctgcttt ccgcaaccgg ctaataccaa gaaactaact
    17101 gccaagatta aaaagagtga tttttttgtt ttcatctatc tgcttccttt ctgaaataaa
    17161 attccttttg ttggagaaaa taaaaaagca aggataaaaa atgcggtaca tgctaacacg
    17221 atcgtagctc ctgatggcca attgtaacta taactgaaaa acacaccgac aactgcactt
    17281 agcataccga ttcctgcagc tgtaatgatc attttcagta aatggttcgt tagcaagtaa
    17341 gccgtggcgg ctggtgtgat caacatggca atcaccaaga ttgttcctac tgtctgtaaa
    17401 ctgactacag ccactaatgt caaaaagaac atcaaaagat aatggatcag cgatgtgttc
    17461 agcccataag cttttgccat tgtcggatcg aatgatgtga tcttcaattc tttataaaag
    17521 aaaaagacaa atatcaagac gattccacta acgagtgctg tcagcaacaa atcgctttcc
    17581 ctaacagcta agacattccc aaataaaata tgatacaaat ctgtcgcact ctgggcatat
    17641 gaaatcaaga taatccctaa tgcaaaaaag gagctaaaga caattccaat cgctgtatca
    17701 tttttcaatc ggctccgctg agtcacgaaa ccaatcactg ctgctgttag catcccaaaa
    17761 gcagtggctc caaagatgta actaaaacca aacatataag aaatggctac acctggaaga
    17821 actgcgtgag agatagcgtc tcccatcaaa gacatccctc gtaaaataat aaaagaaccg
    17881 atgactccag aagcaagtcc tacaagcatg gaagtgacca aagcgtattg caggaattga
    17941 taattcatta atccatcaat aaaggattgg atcatttttt ctctcctcct tttactaaat
    18001 gtctgatgat ctcaccatag gccaattgaa ggttcttcga tgtaaagact tcttgtactg
    18061 gaccggaagc gatcagctgt ttatttaaaa gaataacctc atcgaaatat tcttctactt
    18121 tatgaagatc atgatggacg atcaaaatcg tttttcctga attcttcaat tcctgcaaga
    18181 tgtcaaagat ctttcgttca cttaacgcat caatccctac gaatggttca tctaaaaaga
    18241 tccattctgc tccttgggct agagctcttg caataaaaac tctctgaagc tgtccacccg
    18301 atagttcgct gatctgtctt ttggcatatt cttccatccc tacttttttc aaagcttgtc
    18361 ttgcacgttc tttttctttt ttcccaggtc tttgtccaat tcgtaaagat ggatatgttc
    18421 ctaaaagtac aacgcctaat acatcaattg gaaaggaaag atccaattca cttcgctgtt
    18481 ccacgtaggc tattttttta cggactgagt ttaccggctg tccagaaaag gtgactttac
    18541 cagtagtagc tggtatcaac tctagcaaag atttcataaa tgttgatttt cctgcaccgt
    18601 ttggtccgat aataccggta attttttgtg gatttatcgt caagttgata gttttcaaaa
    18661 cagttttccc ttgataagcg gctgaaaggt cttgaattac aatggccgta cgttccaaat
    18721 cttacactcc tttttaggtt tacctaactt tttatttaaa tatagtttaa ctaaaatgga
    18781 aagcattttc aagtagaatg atgaaaaagt ttttttcgaa ttttcctcgc attttatgaa
    18841 aattttctgt agaatcacat caagagttca ataaggctaa cctaataatg aaaaacatgt
    18901 gttgaaacgt ttgattattt tttcttgttg actcaacaac gaaaggagtt tttgtatgga
    18961 agaagaaaag ttaactcgaa gtcttagtgc ccgtcatatc caaatgatcg cactaggtgg
    19021 aacgatcggt gtcggtctgt ttatgggtgc ctcctcaacc atacgttgga cagggccctc
    19081 agtgatggga gcctatgcga ttgcaggatt ctttttgtat ttgattatgc gtgctttagg
    19141 cgaaatgctt tacgttgatc cttcgactgg ttcttttgca aattacgcga gcgagtacat
    19201 ccatcctgtg gctggctact tgaccgcttg gagtaatatt tttcaatata ttgtcgtagg
    19261 aataagtgaa gtgattgctg taggatctta tatgaattat tggtggcctg atttgccggc
    19321 aattattccc ggaatcattg tcgtcttgtt tcttatgctg gcaaatctta tttctgtcaa
    19381 ggcgtttggc gaattagaat tttggttttc gatcatcaaa gtcataacga tcattttgat
    19441 gatcattgca ggtcttgggg taatcttatt tggatttggt aatcatggtc aggcagtagg
    19501 gatttcaaat ctttggaaaa atggtggatt cttcactgga ggcgtcaaag gatttttctt
    19561 tgcattatcg atcgttgttg cttcctatca aggaatcgaa ctgatcggaa tgactgctgg
    19621 tgaagcagag aatcctaaaa agacgatcat tgaagctgtc cagtccacta tcggtcgaat
    19681 cttgattttt tatattggtg ctatttttat cattgtgagt atctatccgt ggaatcaatt
    19741 gagtgagatt ggttcacctt ttgtacaaac tttttctaaa gtcggcatta catttgctgc
    19801 aggactgatt aattttgtcg tgattacagc ggcattatca ggttgcaatt cagggatttt
    19861 cagcgcaagt cgtatggtct atacacttgc cataaatgga aagatgtcta aaaaatttct
    19921 gaaactatct cgtcacggtg cgccttttta tcctgtcgta gcaatctcag gtggaatctt
    19981 aattggtctg atcttaaatt atcttctacc aatgttcatc aaccagtcag aaaatatttt
    20041 cgttttcgta tacagctcaa gtattttgcc tggtatggtt ccttgggtag tgattttgat
    20101 cagcgagatt cgcttcaaaa agaagcacgc agataaaatg catgatcatc cttttaaaat
    20161 gccactatct ccttatacga attatgtgac acttgctttt ttagctatcg tattgatttt
    20221 catgtttatc aatcctgaaa cgagaatctc attgattatt ggactcgttt tcttgcttta
    20281 catgacgatc cattactttg tacgcgaaaa aagaaccgga aaataaaaaa agaacgaagc
    20341 ttcaccttag tggaagcttc gttctttttt attgctttta tcaattcaat tttagcgtct
    20401 caccgggagt caggaaataa ttctccatgc tcatcccatt cagcgacaag atcgtttcta
    20461 ctggtacgcc agttcgttcg gctatttgat ttggaccttc tccttccaaa actacggttg
    20521 tcgcagcctg tgaagtcgcg ctgttggtat tagtttcaga ggaatctgtt gtgtcttgtg
    20581 ttgttcgagc ttgcatttca gaagcttcta ccgactctgc tgtctcagaa tcagaagatg
    20641 ttgtgtcttg tgtgcttgtt gactctgctt gaagagaggc gctagtctgc aaataaatat
    20701 cggaaatctt agcgacatga ttcgtataat tagccgataa ttgattgatc aaagtatcta
    20761 agtctacacc gtctttattt tctgctgcct gatacgctgc acgtaatttt ctgatccctt
    20821 cgtcggagat tgcttgaagc tcacgtgctt tttggttagc aactgctgat aaaccagcta
    20881 ttccctcttg atttccctga atttctgtct ggtattcttc aatcaatgca ggcgtctttt
    20941 cttcgatctt tttcgaataa tcatttacga ttgcttgaag ttgctctggc aaaccatttg
    21001 ctgttttttc ttcagaagtt tgtgtctctt ttgttttagt tgatgactca cttgtttgtt
    21061 tcgtctctgt tttctttgtc gttgtttccg ttatggtcgt acttttcgct tctgacgaat
    21121 tttttccatg aaaatataga cccgcaactc ctcctgctat gatcaaaaac acggcaactg
    21181 ctgccccaat catgatcatc cgcttacgag ctgcctgttt ttgctgtctt cttcgggtca
    21241 tccgaacatt ttctccccac tcattttcat catacatttt tttcaccacc aatttttatg
    21301 tataatagcg ttatcaaacg cttcatattc ttattgtagc aaataattgt cgctgtgcac
    21361 atttttatct ttgttattta taagaaaatt tcaccagttg agatgattcc aagattgtcc
    21421 gtcgatttct ccttgcagct gcattgttcg ttcgtctatc tcagccatca gtttctttat
    21481 ttcatataag agcgcacggt cttgatattc tgatttttcg cttaattcat ctaattgttg
    21541 gatcagccaa tcatttgtca taatgaagtt cctttcattt cttgcagtgt aaaggtcaag
    21601 tcttcgatct gctcttttgc ctcagtcagt gcttctacga tatatgcttg tttttcagat
    21661 tcttcttcct gcattttctg attttcctgt actctttgaa taaaccagtc aatccgctga
    21721 gtcagcgatg gtttgccttc caatgagaga tactgctgat aagcaattag cttctcccaa
    21781 cgtttttcct gggataaata cataaattgt gctaaaacga atggcggaag ataggtcata
    21841 tggaatttat ttgcgattcg ctgatacggc ctcaaaaatt cggaaatcgt atattcttct
    21901 tttcctccag cttggtattc tttttccgcc acacctgttg tcaatacgat tcctagttct
    21961 ttccctttta atacatgtgc tgctcgttcc aagacattat cttgccaaat ttttaaatga
    22021 gcaggagaac tataccagta aaacggaaac tggaaaatga tccgatcatg ctgggccaag
    22081 agctgtagtt cagtctctat atctattttt ccgtctggat aacttgattc caaatgatgc
    22141 caagtagctg gtaaaccagc agaagcttct ttgaaaaatt gctgatttaa agattcttcg
    22201 atcgttggat ggctaatgat gataagtgta cgcatccctt ttcctccttt ttagtcttcg
    22261 ctttacagtg tagcataacc gccacaagtt tcttttgccg atgcttgtca ttttaaccag
    22321 actaatcagg ttgtccaaga aactgcggtt tgtttttcca actgtttttt caaggtatcc
    22381 aaagcaatat tcttaaaatt ttcgtttttc atacgtttac gatagttcgt agccgtttct
    22441 ccggtactct ttttgaacat tcggctaaat acgactagac tattgaaccc tacttgatag
    22501 gcaatacttt ccacggattc atccgtcaga gaaagaagaa acgcccctcg attgatccgt
    22561 accccgatca aatattcctt gatactggtg cccatatgat ttttgaatac tcgcgacaaa
    22621 tggctccgat tgaccgctaa atgctgggcg atttcctcaa cagacaattt ttcagcataa
    22681 tgtgcctcga tgtatttgac tgtttcaaca gctaaagagg aatgaacctt ttgtacacct
    22741 ccgctgacct ttccgccatc ttttaaaagg actgataaaa aacggtaggt taatgctgtt
    22801 aactgtaatt cattttccaa ggtttcttcc gtatctttca tgcattctaa aataatgtcg
    22861 atatattttt gtatgtcaga agagaccatc gtgtattggt cttctctgaa aagcgactgc
    22921 cgaatgattt cttctgcagc actcccacca aatgaaagcc agcaatacat ccacggatct
    22981 tctccatctg ataagtaaaa cgtcgagtct cctggacgaa tcaagaacag atcacctttt
    23041 ttcaattgga attgttgatt tttcacatga taagatcctt tgccttccat cacgacatgc
    23101 aggatgtaat cattgcgaat agcaggacca aaagaatgaa acggcatcgt tttagagaaa
    23161 ccacaaaaat caaaatacaa ttctttttct acttgttgat gttccttttt taaaaaaatc
    23221 gattgctcca cgtctatccc tccttactcc tttagtataa tgcaacaaaa agttaaataa
    23281 aagtcacaga aatatattaa aaaggaagcg atttcttgtt aagtttaagt cattaaaaga
    23341 tctttgttat tcgttgtaac cggtttactt agtattatat attgaacagg agaggaatac
    23401 agattatgaa aagcaaaatg atccaacgtg tgtcctacgc tttaggcgct tttggacacg
    23461 acacattcta tgttacatta agcacttatt ttatggtatt cgtgacaagc cagatgttcg
    23521 caggaacaga taaggcaacg aatgcaaaaa tgattgctac agttacaagc ttagttgtag
    23581 gtatccgctt agttgaaatt atttttgatc ctttgattgg tggcatcatc gataatactc
    23641 gtacaaaata cggtaaattc aaaccatggt tagttatcgg cggcgttgtt agttccgtac
    23701 tcttggtagc aatctttacc aatttctttg gcttagctac tcataatacg actttattta
    23761 tcatattatt tgcaatcgtt ttcatcttat tggatagttt ttattcattt aaggatatcg
    23821 ctttttggtc aatgattcca gctttgagta cggaaacaaa ggaacgtgaa aaatttgcta
    23881 cgtttgctcg tttcggctct tctctaggtg caaatggtac tactctagtc gttgtcccta
    23941 tcgttacttt ctttacctat cttgttactc ataaacatat tgaaggcgct tcaggctggt
    24001 tctggttcgg tgtgattgct gctgttattt caggcggtac tgcgattatc actgccttgg
    24061 gcacaaaaga agtcgatact gcgatccgtc agcaaaatga aaaagctggg atcaaagatg
    24121 tgttcaaagc agttgctcaa aatgatcagt tgatgtggct tgcccttgct tatatcgctt
    24181 atgcgattgc caatgcagca acaactgccg tattattcta cttcttcaaa tatgtacttg
    24241 gccaaccaac tgaattttgg atcgttggtc tagttgctgc cttacttggt ttagtagctg
    24301 ttccactatt cccaatgctt acacgcgtca tctctcgtcg ctacgtttac cttggaggga
    24361 tcgcaatgat gatttcttct tactttttct ttacgatcgc aggttcaagt ttgccaatcg
    24421 ttatcatcgg gctagtattg ttctacttcc ctcaacaatt gattttctta tccgctttga
    24481 tgacgatcac agactctgtt gaatatggtc aatggaaaaa tggtatccgt aacgaagctg
    24541 ttactctatc aattcgccct ttactagata aaatcgctgg tgctttgtct aacgggatcg
    24601 tagggtttgt cgctatcgct tctgggatga ctggtaatgc tactgctgct gatatcactg
    24661 ctcatggcgt aacgactttc aaagcttatg cattctatgc tcctgctgct ttgatgattt
    24721 tctccgctat tatttttgct tggaagatta cattgacaga gaaaaaacat gcacaaatcg
    24781 ttgagaaatt ggaaaacaaa ttggctccag ctgatacaat ggaagattaa cttcacgatg
    24841 cagtagttcc ttcccattaa gaaaggatct tgaaaatgaa gattacagaa aaagattttg
    24901 gccaaggtta tcacttgata accttggaga acaaaaacaa gctagcactt tctatctctg
    24961 atttaggtgc tagaatcgtc agtttaaaat caaatgatcg agaattagtt ttgggatttg
    25021 acaccgctga agaatatatc gaaaaagatc cgtatatcgg tgcttctatc ggtcgcaccg
    25081 cagggagaat cgaaaatggc cgcttttctc tcaatggaaa aacttaccaa ttagccacag
    25141 atccaaaaac tggacataat ctgcatggtg gtgctcctgg atttgaatta aaaaaatggt
    25201 cttatgtcat tttgaatggc gaaaatgaag cttctgtcat ttttactact acaagtcccg
    25261 acggagaaca tggttttcca ggtacaatgg atgtggaaat ccgctatact ttgacaaaag
    25321 ataatatctg gcgagtgacc tctcgcggca ccagtgacca agatacttta ttcaatccga
    25381 caaatcatgt ttacttcaac ttgacgggag atgcttctca gtcaatcgat caacatgagc
    25441 tttggctcaa tagcgaagct tatgctcctt tgcgaacaga tagtattcct ataggggtaa
    25501 aagaaaatgc tgcaggaagt gctttcgatt ttcaaatacc taaaaaatta gcgtccgttt
    25561 ttgctagtga tttggatcaa aaaaatcttg ttgacggaat cgatcatcca tttttcttaa
    25621 aagaaaccgg cttgggtaaa gaagccgctc gcttgactag ccccgataaa agaatccaag
    25681 tagatattgc tacagatgct tctagtgtgg ttattttcac tgcaaatttc ggcacagaaa
    25741 caccagaaat gcgcaaccga aaactggcac atcacggtgg catcacattt gagactcaaa
    25801 cagctccagg tgcagagcgc ttctcagcat ttggttcgat tcatttaaaa gccggatcag
    25861 tatttgaaac agtgacagaa ttcaaaatca aaacaagaaa ggaataactc atcatggaaa
    25921 tcaaaagaag cttaaacaca aaatttgccg aaatattcgg acctaaaaaa actacacaat
    25981 acttctcacc aggacgcatc aatttgattg gtgaacacac agattacaat ggaggccatg
    26041 tcttccccgc ttccatcact tatggaacgc aaggtgtagc agcacctcga gacgataata
    26101 aaatattagt ttattccaca aactttgacg atatcggtgt cattagtttt actttagatg
    26161 agctagaata tgataaaaaa gcaaactggg caaattacgt aaaaggaatg gtccttaaat
    26221 taaaagaagc tggacactca atcgatcatg ggtttgagtt attagtggaa ggaacaatcc
    26281 ctaatggtgc aggactttcc agtagcgctt cattagaatt attagtaggt gttgtattag
    26341 aagatctgtt tgatttaaaa acagatcgct tggaactagt ccaaacaggt aaaaaagtag
    26401 aaaatgaatt catcggtgtc aattcaggta tcatggatca gtttgccatc ggcttcggtg
    26461 agctagatca tgcaatctta ttagatacaa atacgctgaa atacgaaatg gttccagtga
    26521 aactagatgg ttatgcaatc gtcatcatga atacgaataa gcggcgggaa ttggcagatt
    26581 caaaatacaa tgaacgccgt agcgaatgcg aagaagcctt ggcacgtttg caaaaaactt
    26641 tgcctatctc ttctttagga gacttagatg aagaagaatt tgaatcaact attgaccaaa
    26701 tcggcgatga tacattgata cgcagagctc gccatgccgt ttctgaaaat cagcggactt
    26761 tgaaggcaaa agctgaatta gaagctggga acttagaagc atttggtcaa ctgttaaatg
    26821 attctcacca ctctcttcgc tatgattatg aagtaactgg gatcgaatta gatacactag
    26881 tagatgctgc tcaaaaacaa gaaggtgtct taggtgcacg aatgacagga gctggatttg
    26941 gtggctgtgc gattgctctt gtcaaagaag aaaatatacc tgccttcaaa aacaatgtct
    27001 atgatgaata catgaaagtt gtcggctatg ctccggaatt ttatgttgca cacattggaa
    27061 atgggacaac aaagatcgat ccagaagctg ttcatgtttg ataaaaaaat caacgatctc
    27121 agctaaaaac aaatagataa aaataacctc tcagcaattt ccaaatgtga gccttttgct
    27181 cgtatttgaa aattgctgtt ttttattgac acggaaagag aacgtgtgtt ctaattgtgt
    27241 tgaagaggtg attgttatgt gggcgaatca aaatccggta tttgtttatg aaaaagaacc
    27301 ttcaagagat attttatgta ttgactgcaa atctttttac gcctccgtag agtgcgttga
    27361 aaaggatctt gatcccctta caactaaatt agtagtcatg tcctatccgt ccgattcgac
    27421 agaaacacgg ggtagcggat taatcttggc ttccagtcct gcagcaaaaa aagcttacgg
    27481 catcaccaat atcagccgtg ccagagatct tcccttcccc tatccaagtg atttatatat
    27541 tgttgcccca aggatggcct attatatgga aaagaacaga gaaatcaatt ctatctataa
    27601 aaaatatgca gacgaagcga accatcatgt ctattccgtc gatgaaagct tccttgatgt
    27661 aacaaatagc atggcactct ttggtaaaac aaatgcgtac gacatggcta aactgatcca
    27721 taaagatgtg tttgagcaga ccggaatcta cacgactata ggaattggcg ataacccgct
    27781 acttgctaaa ctagccttag acaatggctc aaaagacgga aagcaaatgt ttgctgaatg
    27841 gcgttatgaa gatgtaccag aaaagttatg gaaaatctca gatatcactg acttttgggg
    27901 aatcggcaaa cgaacagcaa aacgtctgaa acaaatgggt atacattctg tttatgattt
    27961 agctcacgcg gattattatc acttgaaaag caagctaggg gtcatcggta ctcagcttta
    28021 tgcccatgct tggggcatcg atcgaagttt tttgggacaa gaatatacac caaaatcaaa
    28081 aagtatcgga aatagtcaaa tcctagatag agattataca agacgagaag aaatcgagat
    28141 cgttatcaaa gaaatggccg accaagtcgg cactcgcttg agacgatctg cagcaaaaac
    28201 acaagtagtc agcttatggg taggatactc tttaggctat cttgatccaa atggcaagac
    28261 cgggtttcac cagcaaatga aaatcgaagc gacaaatgct agcagagatc tagcagatgc
    28321 tttattgacc attttccacc agcattacca ctaccaggat attcggcgtg taggcgtgaa
    28381 ctgttccgac ctcgtatacg cgacaggttt acagcttaac ctttttgaag atcctgaaaa
    28441 gcagataaat gaagcgaaag ttgattttgt tgtcgatacg attagaaaaa aatacggctt
    28501 tcaatcaatc atccatgcgc atagtctctt gcaaggagga cgcgccatcg cacgtagttc
    28561 tctcgttggc gggcatgcag gtggtatgag tggattagaa ggtggaaaag atgagacgga
    28621 cagcaaaaaa attcattgac tataacgagt atcgagatcg gccatttgga ttaaaatggg
    28681 ggactgcttt tgcgatggaa gaactagtca acggtatcaa aaaaaatgaa gaagaagcac
    28741 gcaaacagcc tcttcctaaa caacaaatga cattgcaaga aatcgatagt atactgacag
    28801 aagccttttt atctcgtaaa gcagtaatca ttcagctcaa tttaaaagat gaatttggaa
    28861 gactcttgga tgaggtcgaa gggcacttca ttggtgaagc ctatgaagat tactttatga
    28921 ttgaccatca gccgatattg tgggaagacg tacggtatat tgagttaaag gaaacaaaaa
    28981 aatggtttgt gacatgagag gttgtgacag aagtgatcaa cttcaaaaaa taagaaagga
    29041 tttttcgaaa attgcttctt aaatttttgt ggaattttgg cttatttttc gaagaagcaa
    29101 cttctggaac aacgtatatt agatatcaga ggttgtgaaa aagtcgctta gttttgagta
    29161 acaagccaag gcgctgattt cacaacctct gtctatctat ccatctattc ggtaagattc
    29221 atgcatgacg tcttctttat ttagtatgta gagccctttt tcttctgacc attcaacata
    29281 aatcagctgt tttacatctg catagccttc tttcttcaag ttctccataa gccatgcttc
    29341 agtcaattga ttggactgaa gcactttatg ctgaatttgc ccttcatcta caagcaggat
    29401 cgataaagct ttgtcttcat ctgatttttt caatacactc atttgaccgg cattctctag
    29461 gataacatgt ttggcatttt caagaaaaaa gcattcttgc tgtcttaaga ttgctcgcaa
    29521 ctgctccatc tcgatatggt tatttgtcaa ctctgtcatg ttgatgacgc cgtctttgat
    29581 tagaacagat ggctcgcctt tgacccaacg attgaccttt tcattttttt ggacgatacg
    29641 ttcgatacca taaatcatta ctgcccaaag cgcaatggca aataggacat gccctacatg
    29701 gacattgtca tcataggttg attcttctaa gatcccacct aaaacaagcg tatagactaa
    29761 atcaaacggt gtgatttctg acatggattt cttcccaagc aaacgtacca ctaataataa
    29821 accgatcaac ccgaacacca gtttcattgt aattgaaaaa atcatttttt ctccccctaa
    29881 ctatctgtta attaaattat atatgctttc tcacgaacac gcatggaata tacaaggaag
    29941 aaatgtatcc ttttactaaa aaaaggactg tgacactgta gtgaccccca aaagttagac
    30001 ttttttggag tggagaaatc cactcctttt ttttatgctc taaaatgata tttttacaca
    30061 accgttacgg ttgttttacg aaattggacg
//
LOCUS       DACEDD010000037        25787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEDD010000037 DACEDD010000000
VERSION     DACEDD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.037"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            438..992
                     /locus_tag="IWO45_001795"
     CDS             438..992
                     /locus_tag="IWO45_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0333271.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="IWO45_001796"
     CDS             complement(1165..1638)
                     /locus_tag="IWO45_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0333272.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="IWO45_001797"
     CDS             1650..2186
                     /locus_tag="IWO45_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0333273.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="IWO45_001798"
     CDS             2226..2597
                     /locus_tag="IWO45_001798"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0333274.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="IWO45_001799"
     CDS             2881..4152
                     /locus_tag="IWO45_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ0333275.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="IWO45_001800"
     CDS             4397..5422
                     /locus_tag="IWO45_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0333276.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5716..6825
                     /locus_tag="IWO45_001801"
     CDS             5716..6825
                     /locus_tag="IWO45_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0333277.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6908..7804
                     /locus_tag="IWO45_001802"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="IWO45_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7820..8644
                     /locus_tag="IWO45_001803"
     CDS             7820..8644
                     /locus_tag="IWO45_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0333278.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="IWO45_001804"
     CDS             8676..9986
                     /locus_tag="IWO45_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAQ0333279.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="IWO45_001805"
     CDS             10134..11393
                     /locus_tag="IWO45_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0333280.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="IWO45_001806"
     CDS             11403..12275
                     /locus_tag="IWO45_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0333281.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="IWO45_001807"
     CDS             12287..13117
                     /locus_tag="IWO45_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0333282.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="IWO45_001808"
     CDS             13157..15340
                     /locus_tag="IWO45_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0333283.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="IWO45_001809"
     CDS             15401..16621
                     /locus_tag="IWO45_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAQ0333284.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="IWO45_001810"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="IWO45_001810"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAQ0333285.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="IWO45_001811"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="IWO45_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="IWO45_001812"
     CDS             19583..19792
                     /locus_tag="IWO45_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0333286.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="IWO45_001813"
     CDS             19785..19934
                     /locus_tag="IWO45_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ0333287.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19969..20753
                     /locus_tag="IWO45_001814"
                     /pseudo
     CDS             19969..20753
                     /locus_tag="IWO45_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWO45_001815"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWO45_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0333288.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="IWO45_001816"
     CDS             22456..22689
                     /locus_tag="IWO45_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ0333289.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="IWO45_001817"
     CDS             22686..23093
                     /locus_tag="IWO45_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0333290.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24230..24748)
                     /locus_tag="IWO45_001818"
     CDS             complement(24230..24748)
                     /locus_tag="IWO45_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0333291.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25115..25363
                     /locus_tag="IWO45_001819"
     CDS             25115..25363
                     /locus_tag="IWO45_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333292.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8786 a         3930 c         4795 g         8276 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    23221 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    23281 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    23341 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    23401 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    23461 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    23521 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
    23581 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    23641 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    23701 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    23761 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    23821 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    23881 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    23941 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    24001 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    24061 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    24121 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    24181 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    24241 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    24301 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    24361 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    24421 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    24481 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    24541 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    24601 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    24661 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    24721 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    24781 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    24841 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    24901 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    24961 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    25021 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    25081 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    25141 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    25201 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    25261 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    25321 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    25381 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    25441 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    25501 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    25561 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    25621 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    25681 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    25741 atgctgctca tttcaactta tatgttttag tgaactaccc ccactta
//
LOCUS       DACEDD010000038        25676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEDD010000038 DACEDD010000000
VERSION     DACEDD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.038"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            171..515
                     /locus_tag="IWO45_001820"
     CDS             171..515
                     /locus_tag="IWO45_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0333293.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IWO45_001821"
                     /pseudo
     CDS             516..935
                     /locus_tag="IWO45_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="IWO45_001822"
     CDS             complement(1027..1377)
                     /locus_tag="IWO45_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333294.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IWO45_001823"
     CDS             complement(1343..2674)
                     /locus_tag="IWO45_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0333295.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IWO45_001824"
     CDS             2930..3496
                     /locus_tag="IWO45_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0333296.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IWO45_001825"
     tRNA            3641..3728
                     /locus_tag="IWO45_001825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IWO45_001826"
     CDS             4062..5036
                     /locus_tag="IWO45_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0333297.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IWO45_001827"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IWO45_001827"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0333298.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IWO45_001828"
     CDS             complement(7451..7897)
                     /locus_tag="IWO45_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0333299.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IWO45_001829"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IWO45_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0333300.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IWO45_001830"
     CDS             8362..8880
                     /locus_tag="IWO45_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0333301.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IWO45_001831"
     CDS             complement(8957..9913)
                     /locus_tag="IWO45_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0333302.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IWO45_001832"
     CDS             10079..10885
                     /locus_tag="IWO45_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333303.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IWO45_001833"
     CDS             10882..11871
                     /locus_tag="IWO45_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0333304.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IWO45_001834"
     CDS             11868..12872
                     /locus_tag="IWO45_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0333305.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="IWO45_001835"
     CDS             13004..13546
                     /locus_tag="IWO45_001835"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0333306.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="IWO45_001836"
     CDS             13566..14264
                     /locus_tag="IWO45_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0333307.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="IWO45_001837"
     CDS             14285..14497
                     /locus_tag="IWO45_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0333308.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="IWO45_001838"
     CDS             14497..15459
                     /locus_tag="IWO45_001838"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0333309.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="IWO45_001839"
     CDS             15477..15872
                     /locus_tag="IWO45_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0333310.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="IWO45_001840"
     CDS             16035..16400
                     /locus_tag="IWO45_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0333311.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="IWO45_001841"
     CDS             16397..18208
                     /locus_tag="IWO45_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0333312.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="IWO45_001842"
     CDS             complement(18270..18437)
                     /locus_tag="IWO45_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0333313.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18685..19674
                     /gene="galE"
                     /locus_tag="IWO45_001843"
     CDS             18685..19674
                     /gene="galE"
                     /locus_tag="IWO45_001843"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0333314.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19686..21170
                     /gene="galT"
                     /locus_tag="IWO45_001844"
     CDS             19686..21170
                     /gene="galT"
                     /locus_tag="IWO45_001844"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0333315.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21194..22174
                     /locus_tag="IWO45_001845"
     CDS             21194..22174
                     /locus_tag="IWO45_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0333316.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22263..23087
                     /gene="tsaB"
                     /locus_tag="IWO45_001846"
     CDS             22263..23087
                     /gene="tsaB"
                     /locus_tag="IWO45_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0333317.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23071..23613
                     /gene="rimI"
                     /locus_tag="IWO45_001847"
     CDS             23071..23613
                     /gene="rimI"
                     /locus_tag="IWO45_001847"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0333318.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23620..24084
                     /gene="rimI"
                     /locus_tag="IWO45_001848"
     CDS             23620..24084
                     /gene="rimI"
                     /locus_tag="IWO45_001848"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0333319.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24081..25097
                     /gene="tsaD"
                     /locus_tag="IWO45_001849"
     CDS             24081..25097
                     /gene="tsaD"
                     /locus_tag="IWO45_001849"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0333320.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8329 a         4398 c         5378 g         7571 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaaaaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attgttcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 catacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcagc caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatat cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt aagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga tcttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaattga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaaaga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atccacctca tttattattt gttatatcta ttatggaaca
    18481 aaagagaaaa aaaggctagt tttacgtttg tttgtaaacc tttgcaaaaa atccttgaaa
    18541 atgtttacta aatgttttat tataaattta ccatatgaat ttacgaaatt ttactttgtt
    18601 tgttttcacc ataaaagtaa acttcgtttt ttaaattata tagaatcaac aaacacaatt
    18661 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    18721 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    18781 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    18841 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    18901 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    18961 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaacgt gaataaaatc
    19021 gtattctctt ctactgcagc aacttatgga gagccgaaag agtctccaat cacagaagat
    19081 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    19141 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    19201 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    19261 atcattttac aagtagctct tggtcaaaga aaagccttgg ctgtttatgg agacgattat
    19321 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    19381 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    19441 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    19501 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    19561 agtgaaaaag cgagagagat tctgggctgg aaacctgaat acacagatat caaagcgatt
    19621 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    19681 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    19741 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    19801 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    19861 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    19921 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    19981 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    20041 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    20101 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    20161 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    20221 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    20281 gaatgaatct agacggtgaa agctggggat tccaatattc cccttatgct tattacaatg
    20341 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    20401 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    20461 taccaattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    20521 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agaagattat ccgcaagtca
    20581 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaact
    20641 tgcttattga agcagctaac tcagtattgg aaaaatggaa agcatattcg gatgaaacag
    20701 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    20761 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    20821 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    20881 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    20941 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    21001 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    21061 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    21121 gagatgaaaa aggacaggca gcgtttgaac gatttactgc gattttataa acgtaaaaaa
    21181 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    21241 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    21301 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    21361 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    21421 ttattattta tctattcgcc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    21481 attgaaagaa acgtgggatt ccattagtga tcttccggca gatggaacga tcgcactagg
    21541 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    21601 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    21661 tcaaaaagtc attgaacact ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    21721 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    21781 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    21841 cgagtcagga tatcaagcag tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    21901 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    21961 tttagtggta ccagaagata tttctgtgat gggcttcaat gatgtcagtg tagcgaaata
    22021 tgtaacgccc tcgctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    22081 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    22141 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt gggaaaatgg
    22201 agtgataccg cagctttttt gtgtttcagg aaggggcaaa atttgcccgc cagcagataa
    22261 tcatgtatta ttgtactagt tttgcctttg gtattttcat cctcagaata aaaaagtatg
    22321 ctacaatgga aggcgagaat gtcatagcag aaaggatcaa atatatgatt acattaggaa
    22381 tagatactgc aaaccaaact ttggcgatcg gtgtggtaga agacgaacaa attcttggac
    22441 aaatacaaac aaatatcaaa cgcaaccaca gtgtaacact gatgcctgca atcgaccaat
    22501 tgtttgcaga tcttcaaata agtccaaaag acattgaccg aatcgcggtt tctgatggtc
    22561 ccggttctta tacagggctt cgtataggtg taacgacagc aaaaacgatt gcttatacat
    22621 tagataaaga gctagtagga gtatctagtc tgaaaaccat cgcggctaat tgcatcggtg
    22681 tggataatat tattgtgccc atgtttgatg ctcgaagaaa aaatgtatac gctggggcat
    22741 accgctggaa agaaaataga ttagaaactg tacttgaaga tgtacatatt tctgcttctg
    22801 atttgttcac acaactacaa aagctgaatg aagctattta tttcgtcggg agtgattgtc
    22861 acaaattctt ctctgaaata aaggaaatat tgcctagtgc gatgattaat acagttccag
    22921 catgggatat tccaagtggt gtaactgtgg ctcaattagg tgctttagtt gaaccagttg
    22981 cagagaaaca agcttttctt cctcgctatt taaaacgagt agaagcagaa gaaaaatggt
    23041 tggagacgca cacacctgga gacgaaagtt atgttgaaaa aatttagagc gtatgccaga
    23101 agcttttttc aagataaaat gatttataca gaaaaaacag tcgaacaaat cgatcgtcgt
    23161 tacgtactaa gagaattgac tacagaagat atcaaagagc tgctggatgt cgaacgagaa
    23221 gtctataatg gcgaattgcc atggacaaaa agtgccttta tatcagaatt gaagtcaccg
    23281 cttcctcatc tatatctagg tatctttgat aatggaagaa tgattggttt tatcggttca
    23341 agagcgttcg gtttagattg ccatatcacg aatattgccg ttcgaccggc atatcagcgt
    23401 aaacgaatcg gttcgctgct tattgacgaa atcgagaact ttgctataat gaatcgctgt
    23461 gagacgatgt cattagaagt gcgtatgagt aatcaagatg cacaaagact ttatcggaaa
    23521 ttggggtttc aagcaacaac gatcaaaaaa agatattaca ctgaaacaaa agaagatgcg
    23581 ttggatatga taaaactttt agaaaatgag tgagattagt tgatttgcag aaaaaaagat
    23641 tttgatgaag cgtacttggc agaatgcgta tggatgatta gcgaaaatgc ttatccacat
    23701 ggttcacctt ggacgaaaat gcagtttttg gcagacatcc aacagcctca ctctgattat
    23761 ctgcttttag ctgaagataa ccagatctgt ggctttattg gatactcgaa agtaatagat
    23821 gaagtcgaga tcacaaatat tgctgttgca gtttcagaac aaggaaaagg gcatgcacgg
    23881 catttgttac aattactgat tacggaacaa ataaaagatt cgctgcatgt ttttttagaa
    23941 gtacgtctat ctaatgaagc agcaagaaaa ctttatgaat cagaaaagtt tcgtatatta
    24001 ggtaaacgga aatcatatta tcgcaatccg acggaagatg cgatcattat gagcagaaag
    24061 ttaaaaacgg agacgatgaa atgaatgaag aattgattct ggcgattgaa tcaagttgtg
    24121 atgaaacgag tgtagcggtc gttagaaatg gaacagagat cttatctaac atcgttgctt
    24181 ctcaaataaa cagtcataaa cgttttggtg gcgtagtacc tgaagtagct agccgccatc
    24241 acgtagaaca aatcacctta tgcttggaag atgcactggt agaagctggt gtgtcggcag
    24301 aagatttatc ggcagtcgct gtcacttatg gaccaggact tgtcggctcg ttgctgattg
    24361 gtatttctgc agctaaggct tttgcttggg cacaccaatt gccgcttatt cctgtaaacc
    24421 atatggcagg gcatatctat gcagcccgtc tggttaaacc attccagttt ccattgatgg
    24481 cacttcttgt tagcggagga catacagaat tagtctatat gcaagaagat ggatcatatg
    24541 aaatcattgg ggagacaaga gatgacgcag caggagaagc ttatgacaaa gttggaagag
    24601 tattaggtct ttcttaccca agcggaaaag aaatcgatca gctagctcat cagggaaaag
    24661 ataattacca ttttccgcga gcgatgatcc atgaagataa ttatgatttc agttttagcg
    24721 gattaaaaag tgcatttatc aatttggtgc ataatgcaca acagcgtgga gaagatctag
    24781 acaaaaatga tttagcagct agctttcaag caagtgtgat cgatgtattg attactaaaa
    24841 cacttcgagc ttgtcaaaat tatccagtca agcaactgat tatagctgga ggtgtagcgg
    24901 ccaatcaagg gttgcgtgaa ggattgcagg atgctttggc aactaaactc cctgaggtag
    24961 aactagtgat tccccctctt cgactttgtg gagataatgc tgcaatgatt ggagcagcag
    25021 cacatgtgga aatgcaaaaa agaaattttg ctagctatca gttgaatgct gatccaagct
    25081 tggttttatc tgaataaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    25141 cccaggaaaa acggcttctc atgtttttga cctggctgtt cttattaccg cgtatgcaag
    25201 cttttgaacg ccgtttaatc aagagttcgt aagcctgtcg tttaactctg attcacaagg
    25261 aacaatttca aaaaacagct cctcatgttt atgaacatta tgacttgtga ccgaagagtt
    25321 gacaggtaca caccgtttaa tcgaaatagg gctgtgacac gtttttgtca aagccctatt
    25381 ttttacatac ttttaatcat tattgttatt ttgctatttt ttacagctta tttatcatta
    25441 tccgttcctc tgattgccct cactgagtaa tcgtttattg tcgaggaata tcgaataatc
    25501 tagatcggat gaggacaaga cgcttcatct agttaatcta acagtatttt ttgcaaacag
    25561 tgaatattac ataactgaaa cacacataga aaatctttga gtcttgtcca gtgatctaaa
    25621 actgccggat ctttcagaag ttttagggag gaaaataagc agtgaccctg atattt
//
LOCUS       DACEDD010000039        24579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEDD010000039 DACEDD010000000
VERSION     DACEDD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.039"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(154..552)
                     /locus_tag="IWO45_001850"
     CDS             complement(154..552)
                     /locus_tag="IWO45_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0333321.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="IWO45_001851"
     CDS             complement(549..1322)
                     /locus_tag="IWO45_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0333322.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="IWO45_001852"
     CDS             complement(1319..2002)
                     /locus_tag="IWO45_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0333323.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="IWO45_001853"
     CDS             complement(2169..3056)
                     /locus_tag="IWO45_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0333324.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="IWO45_001854"
     CDS             complement(3053..3922)
                     /locus_tag="IWO45_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0333325.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="IWO45_001855"
     CDS             complement(4059..5345)
                     /locus_tag="IWO45_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0333326.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="IWO45_001856"
     CDS             5618..6196
                     /locus_tag="IWO45_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0333327.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="IWO45_001857"
     CDS             6196..7125
                     /locus_tag="IWO45_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333328.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="IWO45_001858"
     CDS             7122..8858
                     /locus_tag="IWO45_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0333329.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="IWO45_001859"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="IWO45_001859"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0333330.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="IWO45_001860"
     CDS             complement(9754..10263)
                     /locus_tag="IWO45_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0333331.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="IWO45_001861"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="IWO45_001861"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0333332.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="IWO45_001862"
     CDS             complement(11564..12655)
                     /locus_tag="IWO45_001862"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0333333.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="IWO45_001863"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="IWO45_001863"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0333334.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="IWO45_001864"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="IWO45_001864"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0333335.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="IWO45_001865"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="IWO45_001865"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0333336.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="IWO45_001866"
     CDS             complement(16618..17592)
                     /locus_tag="IWO45_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0333337.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="IWO45_001867"
     CDS             complement(17573..19456)
                     /locus_tag="IWO45_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0333338.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="IWO45_001868"
     CDS             complement(19474..21699)
                     /locus_tag="IWO45_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0333339.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="IWO45_001869"
     CDS             21814..22848
                     /locus_tag="IWO45_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0333340.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="IWO45_001870"
     CDS             complement(22915..23928)
                     /locus_tag="IWO45_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0333341.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="IWO45_001871"
     CDS             complement(24006..24194)
                     /locus_tag="IWO45_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0333342.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7064 a         5341 c         4397 g         7777 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaactaaa
//
LOCUS       DACEDD010000040        24310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEDD010000040 DACEDD010000000
VERSION     DACEDD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.040"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1152)
                     /locus_tag="IWO45_001872"
     CDS             complement(<1..1152)
                     /locus_tag="IWO45_001872"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0333343.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1337..3778
                     /locus_tag="IWO45_001873"
     CDS             1337..3778
                     /locus_tag="IWO45_001873"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0333344.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4108..4812)
                     /locus_tag="IWO45_001874"
     CDS             complement(4108..4812)
                     /locus_tag="IWO45_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333345.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(4999..5244)
                     /locus_tag="IWO45_001875"
     CDS             complement(4999..5244)
                     /locus_tag="IWO45_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333346.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5261..6112)
                     /locus_tag="IWO45_001876"
     CDS             complement(5261..6112)
                     /locus_tag="IWO45_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0333347.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6278..7282
                     /locus_tag="IWO45_001877"
     CDS             6278..7282
                     /locus_tag="IWO45_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0333348.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(7523..7879)
                     /locus_tag="IWO45_001878"
     CDS             complement(7523..7879)
                     /locus_tag="IWO45_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0333349.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            8247..9434
                     /gene="tuf"
                     /locus_tag="IWO45_001879"
     CDS             8247..9434
                     /gene="tuf"
                     /locus_tag="IWO45_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0333350.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(9622..12213)
                     /gene="mprF"
                     /locus_tag="IWO45_001880"
     CDS             complement(9622..12213)
                     /gene="mprF"
                     /locus_tag="IWO45_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0333351.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(12239..12958)
                     /locus_tag="IWO45_001881"
     CDS             complement(12239..12958)
                     /locus_tag="IWO45_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0333352.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            13278..13799
                     /locus_tag="IWO45_001882"
     CDS             13278..13799
                     /locus_tag="IWO45_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0333353.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            13858..14718
                     /locus_tag="IWO45_001883"
     CDS             13858..14718
                     /locus_tag="IWO45_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0333354.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            14825..17218
                     /locus_tag="IWO45_001884"
     CDS             14825..17218
                     /locus_tag="IWO45_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0333355.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            17400..19241
                     /locus_tag="IWO45_001885"
     CDS             17400..19241
                     /locus_tag="IWO45_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0333356.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            19265..20374
                     /gene="rpoD"
                     /locus_tag="IWO45_001886"
     CDS             19265..20374
                     /gene="rpoD"
                     /locus_tag="IWO45_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0333357.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            20594..22693
                     /locus_tag="IWO45_001887"
     CDS             20594..22693
                     /locus_tag="IWO45_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0333358.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            22889..23752
                     /locus_tag="IWO45_001888"
     CDS             22889..23752
                     /locus_tag="IWO45_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0333359.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            23857..>24310
                     /locus_tag="IWO45_001889"
     CDS             23857..>24310
                     /locus_tag="IWO45_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0333360.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         8126 a         4299 c         5037 g         6848 t
ORIGIN      
        1 tattttgtag taacgaatat cattttcgat ctttgtaatc gaaaaaggta catctgaagt
       61 atattctgca cattctccgt gttcaggaag cgtctcactg aaaatcgttt catattcgtc
      121 aacagtgatc cgtgttcgag aatctagcat ctcttgatga gattgtgcga agagatattc
      181 ttgatagttt tcttccagat aacctgtaaa aaattcactt actgcaccag aaccgtagct
      241 gaatagaccg atgcgatctc ctggctgtag tgatttcgaa ttttccagta gagaagttag
      301 ccccaggtat aatgaaccag tgtaaagatt accgattcgt cggctgtaac ggatgctttc
      361 ttcatagcga gccataagac gttcctgatt ttcttcgtct gtttggtcta atacgctttg
      421 caatgccttt tttcccatct tagtatacgg aatatggaaa gcaatcgcct gataatcttc
      481 gagtcctcga cccgacaatt ctttatgtcg attccaaact ttttggaatg attcgatata
      541 cgtagaatta gataaaggac catcaacaac aggaaattcg ctataatctg gacgccagaa
      601 atcatagata tcttctgtca gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt
      661 ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc acttcaccac cgcttgccaa
      721 gccgtaacga gcaatatcac ttgctatgac tagtactttt cgttctggat gatttttgac
      781 atattctttc gccatgtgca aagctgcggt gccaccgtag caagcctctt ttacttcaaa
      841 agaacgagca aaagattgga ttttcagtaa atggtggata attaccgcac tcgcttttga
      901 atgatcaatc ccggattctg ttccgacgat gaccatgtcg attagctcac ggtctttttc
      961 tgttacgatt ttgctggcag catttgctcc tagtgtaatg atgtcttcat ttgcacgatt
     1021 gactgccatt tggtcttgac caatccctat atggtatttt gcaggatcat ccccacggct
     1081 ttctgccaac tctgtcatat ctaaatataa attaggaata aaaaaggaaa gacgatcaat
     1141 ccctattttc atttcaacac ctctttactt atgataaaaa acttacttta ctaaggtatc
     1201 ataaaaagat taaaagaaat ataaaaaaga gaatatttaa ggattgttaa aatttaatct
     1261 tagaaaaaaa taactggaac aagagctatt tgtttttaca gcaaagaatg gtatattttt
     1321 gtaggaggtg gcttacttga aagaagtcgt tatgatagat gctgcaagaa cgccgatagg
     1381 aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa ttaggcacac ttgtgactaa
     1441 aggattatta gataaaacaa aactgaaaaa agataaaatc gatcaagtaa tatttggaaa
     1501 tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag attgctttga acagcggtct
     1561 ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt
     1621 gatcttagca cgccagctga tccaattagg tgaagcagaa cttgtgatag ccggcggaac
     1681 agagagtatg tctcaagcac ctatgctgaa acagtatcag tcagaaacaa atgaatatgg
     1741 tgaaccaatt tccagtatgg tcaacgacgg attgactgac gcattttcaa atgcacatat
     1801 gggattaacc gcagagaatg ttgcaacaca attttctgtg agcagagaag agcaggatcg
     1861 ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg tattttctga
     1921 ggagatcatc ccagtcaaga tttctgatga agacgtgtta tctgaggatg aagcagttcg
     1981 tggaaatagt acattggaaa aactgggcac gttacgtaca gtattctcag aagaaggaac
     2041 tgtaacagca ggaaatgctt ccccgttgaa tgacggtgcc tctgtggtga tccttgcatc
     2101 caaagaatac gcagaaaata ataatctgcc ttatttagca accatcaaag aagtagcgga
     2161 ggtcggtatt gacccaagta tcatgggaat cgctccaatc aaagccattc aaaaattgac
     2221 agatagatca ggaatgaatt tatcaacaat cgatcttttt gagatcaatg aagcatttgc
     2281 agcttcttct atcgtagtta gccaagaact gcagttagat gaagaaaagg tcaatatata
     2341 tggaggagca atcgcattag gccatccaat tggtgcaagc ggtgcacgta tcttgactac
     2401 attagcttac ggattgcttc gtgaacagaa gagatacggt attgcttcat tatgtattgg
     2461 tggaggttta ggcttggcag tcttgttaga agcgaatatg gagcaaacac ataaggatgt
     2521 gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg cgcagtcaat taatagaaaa
     2581 aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa caaacacttt ctgaagagct
     2641 ctccgatcac atgatcgaaa accaagtgag tgaagtggaa atcccaatgg ggatcgcgca
     2701 gaattttcaa atcaatggca agaaaaaatg gattccgatg gcaacagagg aaccatctgt
     2761 cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca
     2821 gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa
     2881 tgcagttaat catcgaaaag aagagctgat tctttgtgca aatgaaagtt atccttcaat
     2941 cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg gaattcatgg ggagtttcca
     3001 tgcatactta tctattgatt ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat
     3061 caactcgatt cttgagagtg tggcaaacaa gttacgggag tggttccctg aagaagagat
     3121 tctattcagt attttaagta attttgctac agaatcattg gcttctgcct gttgtgaaat
     3181 cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca
     3241 acaggcaggg gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca acaaagggat
     3301 catgaatggt atagaagctg tagtggcagc tacaggaaat gatacacgtg cggttagcgc
     3361 ttcgatccac gcctacgcgg caagaaatgg attataccaa ggattaacag actggcagat
     3421 caaaggggat aaactagttg gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg
     3481 agcttccaat atcctgccaa aagcaaaagc atctttagct atgttagata ttgattcagc
     3541 caaggaacta gcacaagtaa ttgctgcagt agggttagct caaaacctgg ctgctttgcg
     3601 tgcattagtt acagaaggaa tccaaaaagg ccacatggga cttcaagctc gttcattagc
     3661 gatctcaatt ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc
     3721 agaaaagatg aaccagcaga cagctataca aatattggaa aaaatccggg aaaaataaaa
     3781 aagcaatatc tttagaagaa taaagcaaga tttacagtta ttttatttac tgcagatctt
     3841 gcttttttct ctatcagaaa atatgtttat tattagaatt ttttatagtt aaggttaggc
     3901 atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc gcctaacatt
     3961 gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg atgttagaag
     4021 gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg gattttaaat
     4081 cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg ctgcaaattg
     4141 ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg ctttttcgat
     4201 aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt catcagcatt
     4261 tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag gatactcagc
     4321 aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg agcgtgtatt
     4381 ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga ctgtttcttc
     4441 tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc gttgatcatt
     4501 ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa tcccgttttc
     4561 tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca cgttgatccg
     4621 tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt ttttgggatc
     4681 aagcatgact tgtaacggca aaccataacg tcgcaagctc tcttcttcta gagaacggaa
     4741 taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt tttgtttctg
     4801 ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga aaaaaacagc
     4861 caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac agaaaaattt
     4921 gcgtattttc ccgttttttg aaagaaaaat aaaaaatgga acggattatg tatatataca
     4981 taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta gttcaggtga
     5041 ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga accgtttttt
     5101 cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt ggattttctc
     5161 tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt ccgagagaga
     5221 attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg gtagtatctg
     5281 ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt tcttcttctg
     5341 ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc actgacttag
     5401 gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt
     5461 catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca gtgcctaatg
     5521 gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca gttgtctgta
     5581 attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct gtttttgcta
     5641 gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt ttacctgctt
     5701 catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa
     5761 aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt tggagcttct
     5821 tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca ttccataatt
     5881 ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct tggccgacgc
     5941 tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca gccagcgctt
     6001 ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg aatcctacta
     6061 ccgggatttc gtaaccattt gatagacgat aagtgcttgt taatgaattc atactttcag
     6121 ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt
     6181 tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat
     6241 tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt taaaaaaagc
     6301 aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa aagagctgga
     6361 tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa tctcttctgc
     6421 tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc
     6481 tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg atatctacca
     6541 agaaagtgaa gaagcaccta gtgcagatgt atttgttgtc ttgtatgaat tagatacagt
     6601 gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact ttgttgaagc
     6661 agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag gggcgaaaac
     6721 acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg aattaattga
     6781 aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag tgatcgaaag
     6841 tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct
     6901 tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag aagctgtcta
     6961 tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa aagtcttcaa
     7021 ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag gatttgtccc
     7081 agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa
     7141 attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc gcaacccaga
     7201 tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca aaaatgtgga
     7261 tgaagtattg aatcgtttat aaaaatgact agaatattag gtgttcaagt accagtgatc
     7321 agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataacaa aaaaagagcg
     7381 tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
     7441 agaagcaact ccttcttatt tcttggagtt aaacgcttct gtcccagcct ctttttatct
     7501 aataaaattt taagaccatt tactaattat cgattttttc tgccgcattt ttaggcactt
     7561 cagaagcctt tatttcttcc cagcttaata cacctttacg aggattgact ttgagtttaa
     7621 gatacgcatt catctttaat ggatgttctc tttcttcgcg aagcgtcacc tgtttttttt
     7681 caccatcttt attataagcc gcttgcttgt aggtataagt cgtgaatttc tctccgttat
     7741 ctgcttgggt agctgacatt tcgccattcg tcgtgatttt tgtataataa gcttcgccgc
     7801 cgtaaaaata atgataagct gcaaaacttc cgccgagaaa tatgcctaaa acgacaagta
     7861 cgccaattaa ttttttcatg tacttcacct ctttccttat tattaacgaa atagaagatt
     7921 tcgtctattg aaagttgttg gcaacagctg aattttaaac ttttcctcat tttgttctta
     7981 ttgcttcaaa agaaagttaa ggactatctt gaagtttttc tcaaaaaaca cattttttta
     8041 tttataaaag gtactttttt cgcgtataag acagacgagt gctcttagtt gactttgacc
     8101 aatattgacg tataataagc ttagttcgaa tcttacaaaa agtaagtcgg acgtattctt
     8161 tttgatttag tcaaaaaaag actataataa aaaggtgaga gctgaaattt ttcagtgatc
     8221 tatattattt aggaggtttg taaattatgg caaaagaaca ttatgacaga agtaaaccac
     8281 acgttaacat tggtacgatt ggacacgttg accatgggaa aactacttta actgctgcga
     8341 tcactacagt attaggcaaa aaaggcctag caaatcctca agactatgct agtattgatg
     8401 cagctccaga agaacgtgaa cgtgggatta cgatcaatac cgcacacgta gaatatgaaa
     8461 cagaaaaacg ccactacgcg catattgatg caccaggaca cgcggactat gtgaaaaaca
     8521 tgattacagg ggcagcacaa atggatggtg caatcttagt tgtttctgca actgacggtc
     8581 cgatgcctca aacacgtgaa cacattttat tgtcacgcca agttggtgta aaatacctga
     8641 ttgttttctt gaacaaagtt gatttagtcg atgatgaaga attgatcgat ttggtagaaa
     8701 tggaagttcg cgagttattg agcgaatatg gtttcccagg cgatgacact cctgtgatca
     8761 aaggttccgc attaaaagca ttgcaaggcg atccagatgc tgaagctgct attatggaat
     8821 tgatggatac agtagatgaa tatatcccaa caccagaacg tgatacagat aaaccattac
     8881 tattgccagt ggaagacgtc ttctcaatta caggtcgagg aactgttgcc tcaggtcgta
     8941 ttgatcgtgg tgctgttcgt gtcggtgatg aggtagagat cgtagggatc aaacctgaaa
     9001 cacaaaaagc agttgtaaca ggtgtagaaa tgttccgtaa aacgttagat tacggggaag
     9061 ctggggataa cgtaggcgtg ttgttacggg ggatccaacg tgacgatatc gaacgtggac
     9121 aagtacttgc taaaccaggt tccattactc cacatacaaa attcaaagca gaagtgtacg
     9181 tgttgacaaa agaagaaggt ggacgtcata ctccattctt caacaactat cgtccacagt
     9241 tctacttccg cacaactgat gttacaggaa caatcacatt gccagaagat acagaaatgg
     9301 tcatgcctgg tgacaacgtg acgattgatg ttgacttgat ccatccaatc gctgttgaaa
     9361 acggaacgac attctcaatt cgtgaaggcg gacggacagt aggttcaggt atcgttacag
     9421 agatcgaagc ataagaaacg cgactcgaat attttggtaa tgaaaaatgt ttaaaataaa
     9481 agagcggtcc aaacaggatt tctgtttgga ccgctctttg ttcttcaatt gtataagaag
     9541 agattgtaga agcatgaact ttcgttttac ttcagtctct tttattcatc atgagagtgt
     9601 tcatctatcg gtatgcagtt tttaatactt tcttcgtatc cattttttca aaccacttat
     9661 tctaccttgt gtctgctcaa ctggcgcatt atcaataatc agcaaagcaa tcataacata
     9721 tgcaatccaa ctatctctag aatatagagt ataacgtgag acccagtttg tagcgtactt
     9781 gcttttatat tctcttaatc catggaaaga ataaaaatga gaaccaaact cgtaaaccaa
     9841 gtatgcaatc ctttcctgta aaaaactctt tctggaagtt ccgacatttg aaagaggggc
     9901 catccctaaa ttgaaccatt ggatatgttc ttctttcata taggcaaaca aatgcaagaa
     9961 caagaaatcc atactgccag aaggtgcttt tttagaatca tatcgcatca gatcaatcgt
    10021 tcctacttta ttgttcgtgt aagtaggcat gatcgttgca aaagcgacaa tttcttcttg
    10081 ggtatttttg acaactgcaa taggcgctct ttgtagataa tcttcagaga aaaagcctaa
    10141 agaaaaccct ttttcctttc gtgaacccaa ccaattatca gagatatttt tgaaagtact
    10201 catttgctca gtagaaaatg gtggttgtaa gacatcaaat gtaaatcctt ctcgttcgat
    10261 ccgattcaag acagcacgag tgcccttcat ttttttccct gaagttgtaa aactattcag
    10321 gtctacataa gcttcttccc ccatcttaat gaaatcgtag ccgaattcat gcaaaatcat
    10381 aacaatctct tcgcttgttt cgtagaaaac tggtaaatag catagtcgat ctgtttcttc
    10441 gataaaagct tcgattgctt ctggaaaatc ttctttctta ccagaaggat cacccattac
    10501 aatacattta ttattgaagc aagcgaattg gagaagaaca gttggttcac cgtctttttc
    10561 ataggcaaac atccgcttat cttttaagaa gatcaattga ctatcggagt ttcctccata
    10621 agttgtaagg atcttcaggg ccttttcttc attaaaggct tcgccaatct gtttcttttc
    10681 tccttgaagg aaatgaacaa atagaacgat catgaaacta acagcgatga tggccaacaa
    10741 tcccgaaaac caaatctttt cagaaggaaa taagaaaaag gaaataaaat ggtgacgccg
    10801 atgagggaaa tccggcagat tgtatacccc aataacgatg taaagcaagg acagtgcacc
    10861 aataatgatt ccatcgatcg tacgccactc ccatgaatag atcaattgtt cccgaaataa
    10921 ttcgttttta gaagcaagaa tgatcagaag cagaatagac aagaaaacca ccggtgtgaa
    10981 actaaaatca cttaataaaa cataaagtaa agctagaaca atcaagaaga ttgtaggcaa
    11041 atatgctcgt ttcacacgtg ctgcgatccc tctgcccata atgattaaaa gaaatcccaa
    11101 tagtatacta ggaaattgga taataaaatg aaatttcaat gggttcaaac tatgaagcca
    11161 gcgaaactct gtaaaagctt gcgggatagt ggcagaaagt actagcatga ttccagaaaa
    11221 gtataataag acaaccacga ttttatgcgc aatttctgtt gctaattgtt tagggatacc
    11281 agaataacgc tgatcaaaag tggaaccgat atttttgaag aaaaagacga ttccaatcaa
    11341 aaacggaatg atgtagtaga acaggcgata gagcaagatc cacacaacga ctatttctct
    11401 tggcacacca atagcggaca acccgataat catcattaca tcgaaactcc caatttctcc
    11461 tggaatcata gacacgattc ctatcactga agcagcaatg aataatggaa ttgtttgcca
    11521 cagatcaatc cggatttcca ttaaataacc aacagaaagg aaactaaaca ggacaccggt
    11581 ccattccaac aatgaaacta gcaatagctg tccttgagtt ttaagtgagg taccaccaag
    11641 gaatcctttc tttttgaaag tagtaaataa aaagacgaca ggaaaatata gaccgccgcc
    11701 gatcaaccat atccaatatt gcttcaaaaa aggagcaact ggcgtgaaga ctacaagtaa
    11761 aaaagaaagc agactgtaaa tagaaagtcc agccatcaaa aacaagaaaa ctttcgataa
    11821 tgcttggatc acgtttctgc tctctttctt ttgtccatat agttcagagc gaagccccac
    11881 actaacaaac ccgccgaaac cggcaatatt gttgatcgta ttgatcagcc agcttgtctc
    11941 aaacaggtaa cgaggattct gcttttgttc aagcatccga ttcaaaataa tatcataacc
    12001 aaccataggt aacacagaga taagaccaat caatagcatt agtccgcttt tccatacagg
    12061 tatagtggca aacgtttctc ctaattgctg gatagataga gttttaccga tggacatcaa
    12121 ttcaccgaca ataatgacaa tgacagaaat caaaaagatt gttttgaaca gtcctaggtg
    12181 gtttttcagc cattgtatgg tattttttaa caaataaatc accttctatt cctaatagtt
    12241 atgtttcaga aagaaattga agaacttctc gattgaagcg ttcaggatct tttctagcta
    12301 attcatgtcc ttgttctttc acaagcacaa aggaagcttt tggtatagtt ttagctatat
    12361 acaaagaatg ttttagcttg attacatctt tttttcccac gatgatcaaa gtgggggagt
    12421 tgattttttt taaatcattt tctgtaagcc cgatgtcatg cagcaaaagt ttgataacta
    12481 aaagattttt gcgaagactt gggcgaaata aagagagtaa ccatacccat gcataatgaa
    12541 tattactgat gactctggca gaaaaacgaa cgcctttaac aagcgtgttt ccagaattta
    12601 aaatcaatcg atgaactttt ttagggaagc tgctggcaaa taccagtgct agatttgctc
    12661 catcactaaa tccaagaaaa tctgcctgat cgattttttc cagcaacatg atcgtattca
    12721 gatcctcggc cattaactga aaattcaaca tagatgcttc gtttgtcgaa cgaccatgtc
    12781 ctcggctatc tactagatac acggtatagt agcgttcgag tacagggacc tgttctgaaa
    12841 aaaagcggcc gctgccgtca ttgccatgca gtaaaaagag agggaagcct tgaccgctct
    12901 tttcatagta aatttcgctc ccatctggca tttttgcata tttcttttct tttttcacat
    12961 aatcacctac tgtgtaaagt ataacgatta tatcagtcat tgggcaattg tttttactga
    13021 aaaacaacga tcattttcgt aagatgtcaa aaaatacagg aaaattcatt ttttcttaag
    13081 gcgtacacag aaatcaagga agaaatagcc aagaagataa agaaatagta agataaaaaa
    13141 agatgtcaaa ataactaggt gatgaaatga agcgaaaacg attatctatt atccgtattc
    13201 ttttttacat gatcattctg acggttattg gtgcggtgtt gtttttatga ggaagaactt
    13261 atcgttcggt aaaacgagtg gaaagctatc aaatagaaat aacaacagct gcaaaaaatt
    13321 atcatatgga aaactacgag aatttgatca aggcgataat attgaccgaa tcaaaaggaa
    13381 aagggacaga tctcatgcag agctcggaaa gtctgcatgg agaagctgag atcattgata
    13441 atccagctga cagtattgat caaggtgtca aatatttggc ggaaatgata gtagaagcaa
    13501 agaaacaagg atgcgattta tggacagctg tccaagcata taattttggg aaagattata
    13561 ttgcctatgt aaaaaatcat ggcggagaaa acacagtaga attagcagaa gcgtattcta
    13621 aagatgtgct gagcccactt ttaggaaatg aggaagggaa acaataccga tattggcgga
    13681 tccagtcctt gctttacaat ggcggctatc tctatcataa tggaggtaac atgttttatc
    13741 ctgatattgt caaaatgaat gaaaaaataa tagagaccta tgaaaaagtc tttaagtaaa
    13801 atagcaggaa atgttcaatc atgaataaaa ttaaatgaaa gaaggagtgc gcaaatagtg
    13861 gaaaaaaggc aaaaaatgga aaatcaagaa gtggggtaca caaccaaaga ctggatgtta
    13921 cggtttgtga agggaatgtt tatcggttca ggatttatct tgccaggagt cagcggtggt
    13981 gcattagcag ctgtctttgg catgtatgag cggctgattt cttttttagc ccaccttaca
    14041 aaaaatttca aagaaaatgt tttgtttttt ctgcctgttg gtctaggcgg agtgacaggc
    14101 atctttctac tttctttttt agtcagtttt ttattaggtg cgtatgaaac aacgatatta
    14161 tggttttttg tcggatgtat cgctggaact gttcctgcgt tatggaaaga agcagggaaa
    14221 aaaggaagag atacttctga tgtgatcatc atggttgtta cttttgttct ggcttatttg
    14281 tttttattat gcggcgctca attatttgac gggcaagtcg atcagaattt tggaacatgg
    14341 ctgattgccg gcggattgat tggtttgggt atgatcgtcc caggattgag cccttctaat
    14401 tttttggtgt atatgggatt gtataaagcg atggctgacg gatttaaaga agtacgactt
    14461 gaagtgattg tgccgatcgg aattggtggg attctttgtg ttcttggatt gtcaaaaatc
    14521 atggattaca ttttctcaaa ggcatatagc aagctattcc atttcatttt aggtgtagtt
    14581 tttgcttcaa cgattatgat cattcctacc gattacgcgg gattgggagt tgctggggta
    14641 ctagcttgtc ctgttctttt tatagccggt acagcactag gttggtggat gagccgtttg
    14701 gaggaacaat ataagtaaag atagattgat aataaaaaca gcacacgggt gattcaaaca
    14761 aaaaggttgg catctttttt gtaaaatcat tcgtgtgttt ttctataaat tttataaaaa
    14821 caagttgttt ttaatcttgg aacacgctag acttaaacac gtgattattt ttgaaagttg
    14881 gcggaaaatg gagaaaaaga aaatgaatgg acttacacag caagaggtag ttcagaaaat
    14941 ggcttctggc cagcaaaatg actaccagga agatgcatca aaaagtacaa aacaaatatt
    15001 cagcgataat atattgacgt tattcaattt tctgaacttt ggtattggga tttgtctgtt
    15061 atttgtaggt gcgtattcca acatggcgta tttagcaatt attgtcgtca atatcgttat
    15121 tggaatttat caagaaatac atgcaagaaa tcttgtacgg aaattatcga ttgtttccca
    15181 atcagaagta ctagtgatcc gggacggtaa agaacaatcg atttctacta aagagctggt
    15241 attaggagat ttggtaaaaa tcggagcagg agaacagata ccagccgatt tacaggttat
    15301 atctggacgg gcagaagcaa atgaagctct tttgacaggc gaatctgatt tgattgtcaa
    15361 agaaactgga gctgaattgc tttctggaag ctttttgaca agcggttctg tcatggctga
    15421 agttatccga gtaggtgcag ataattatgc tgtccgcttg actagcgagg caaagactca
    15481 caaagctatt cgttctgaac ttgtagattc gattcgcaaa gtatccaaat tcactagttt
    15541 cgtgattatt cctttgggga tcatactatt tttggaagca ctgttcatcc gcgatgcttc
    15601 tatcaaaatt tctgttattg catctgcagc agctttgtta ggcatgcttc caaagggttt
    15661 ggtattgcta ataagtattg cattaacgac aggggtaacc aaactggcaa aaaaacgaat
    15721 ccttgttcaa gacatgtatt caattgaaac gctggcccat gtcgatacat tatgcttgga
    15781 taaaacaggg accattactg aagggaaaat gagtatccag cgggtagaag cactacatcc
    15841 gctgtacgag aaagccatcc cagaaattat ggggacgtat ctggcagaaa gcagtgataa
    15901 caacatcact atgcgggcta tgagagatta ttttgagctt tctaatcggt atgatgtgac
    15961 aaacaaagtc gcattttctt ctgatcgcaa atggggcgcg atggaatttc cagaactggg
    16021 agtcgtgtac gtaggagcgc ctgaacgttt ggtggaagaa gatcggcttc cggacagtct
    16081 aaaaacagcc caagcgaatg gctatcgcgt acttatgttg gggatcgctc caaatcagca
    16141 attgaacgaa gaaaaaccag catttgttga gccggttgct ctgtttgaaa tcgatgatcc
    16201 aatccgaaaa aacgctcaag aaacactagc ttatcttcat gaggaaggtg tggatctgaa
    16261 agtgatttct ggagacaatc cttttactgt ctccaatatc gctagacgtg caggattacc
    16321 gaattatgat gcgtatgtcg atctttctca aattactgaa gaaatggaag tgcgagaagc
    16381 tgctcatcgc tatacggttt ttggacgagt gagtccgcag caaaagaaat tgctggtaaa
    16441 tgaattgaaa gaaagcggca gaacggttgc aatgacaggc gacggtgtga atgatgtttt
    16501 ggcgttaaga gaagcagatt gcagcattgc tatggcagaa ggtgatggag caacacgtca
    16561 gattgcgaat ctggtattgt tggactctga ttttactaca ttgccagaag tcctgtttga
    16621 aggacgccga gtggtcaaca atgtgacgaa agtctcaggc atctttttta tcaaaacgat
    16681 ttattcattt attttatcga ttatatgtgc cgttacagca attggatttc cgtttatccc
    16741 tatccaaatc acgctgattg atttagcgat tgaagggtat ccttccttct ttttatcttt
    16801 tgaacaagat aaaagaaaaa tcacttaccg ttatttgcct acagcactta ttaatgcttt
    16861 accaaatgcc ttgcttgtag tagtgaatat cattgcggtg tatctacttg gaaaaacatt
    16921 cgcatgggga aatgctgaaa ctactacttt gatgtattac ttgctgattg ggatcagttg
    16981 catggcagtt gtaaaagctt gttatccctt caacccattg cgcatcttcc tagcagtaac
    17041 gacgatcgta gggatatatg tagctgcaat gctgttccat ggaattttgg aagtaaactt
    17101 agggttggga gagagcgtag gatacttctt tgtattcatg ctcgtaaata ttgttcttcg
    17161 agtattgtac tggaaggctg atattcaaaa tcagttactg aaaagagtag caaattgatg
    17221 cttaatgaga tagaaatgtg ttatacttga aaaagcaatg aaatatgata caatcaaaca
    17281 tgtataattg tgtcctttaa aatcgcactg atttcagtta gtgcgatttt ttaccggtta
    17341 ttctcctgaa atatcttctg ttaaacccaa aagatatcaa tagagcaggg ggtgaaaaaa
    17401 tggctcaacg gattcctcaa gaggttatcg aaacagtacg cagtcaaacg aatatcgttg
    17461 aggtgattgg acaatatgtc cagttgaaaa agtctggtaa aaattacttg gggctgtgtc
    17521 cttttcatga agaacgtaca ccgtcttttt ctgttgcaga agacaagcag atttttcatt
    17581 gctttggttg tggtaaaggc ggaaacgtgt ttacttttct tcaagaacta gaaggtctaa
    17641 gttttccaga agcagtggta aaagtggcag acttcgaaca gataccgtta gaagatcaat
    17701 ggcgtcagcg tactatagct gtccaaaatc ctgaatctgc tacggggaaa ctgattgctg
    17761 ctcatgaaaa agcagcggaa atctatcatc acatgctatt gcatacaaaa gctggacagc
    17821 cagctttaga atatttgcaa agtcgtgggt tgtctttaga attaattgaa gaattcaaca
    17881 taggttttgc acctaatgag cgttcttttc ttcagcaagt attcaagaat gaatcaattt
    17941 caaatgaatt gttagaacgc tctggattgt ttgtcacgca tgatgatggc cgtatgtctg
    18001 atcgttttta tcaaagagtg atgtttccac ttcgtaattc tcaaggccag acgatcggtt
    18061 tttcaggacg ctggcttgca cctgaaaaag ggcaggaaaa agatcagccc aaatacttga
    18121 atagtccaga aacagaactg ttcaataaaa gacaagtttt atttaatctt gataaagcaa
    18181 gaagcgatat ccggaaaaac ggagaaattt tgttgttcga agggtttatg gatgtgattg
    18241 ctgcatggca ggctggtatc aaaactggat tagcttctat ggggacaagt ctaacagctc
    18301 aacagatcca acaaatggaa aagctgacga aagaattggt cttttgctat gacggagaca
    18361 acgcaggttt tgaagcaacc aatcgaggga ttgaattgct tcggcaaaac agtcgattag
    18421 cactttctgt cgtagtcgtt ccagaaaagc ttgatcctga tgaatatctc cgtaagtatg
    18481 gagaagcatc tttccaagaa ttagtggctc atgggcgcga aacagtcttt acttttaaat
    18541 cacgttattt aaaacaaggg aaaaatttag ataatgaaaa agacaagatc gaatacctcg
    18601 aagaattact agttgaactt agccgagttg tttcaccaat cgaacaagat atgtatctat
    18661 ctcaactgtc aacagagttt cagatcggca gagagacgat acaaaaacaa ctgcgcgatc
    18721 ttcgaaggac aaatcagcaa aatcgtcaag aacctcagga aagtcaacac actcaggtta
    18781 cgagacaaga aactgcacgg aaaaagagac ctttgacgca agtagaaaag gcagagcaga
    18841 ttctattata tcgtgcattt aaagaagttt ctgtccgtaa tattttaaaa gaacgagaat
    18901 ttcagtttat tcatgatgca tatgcagaag tttatttttt attcgatgca tatgtctcac
    18961 aacattatga attcaacctt gcagagtttt tagacttttt gcaagatgaa aatttaaggc
    19021 ggttggtggt ggaaatcgaa tatttaccgg tggcagaaga aagttcgcca cgagaaatcc
    19081 aagacttgct agatgtgatc agcaaatctg gcttagctga tgaaataaca ttaaaaaaac
    19141 aaatgcagca agaagccaga cgaactggaa acaaacagct tgagcttgaa ttgacgattg
    19201 aaatcatcaa tttaacgaaa cagttaaaac aagccaaata gctactcaag gggggccttc
    19261 ttttatggca aatggaacag ataaaaaaac gtatgaagca gcagtagcag ctttcatcaa
    19321 agaaaacaaa cccaaacgac aagttgtata tgatgattta tccaatcagc ttgctacacc
    19381 attcactttg aatgcagatg aaatggacaa attgatccaa aagattgaag acgcaggtat
    19441 cagtgtggtg gatgaaaatg gtgatccatc aattcacagc ttgaagagtg ctgagaaaaa
    19501 agctgaacaa gcaaaaacag aagatctttc tgcgccaacc ggtgtaaaaa tcaatgaccc
    19561 ggttcgtatg tatctgaaag aaattggccg tgtctccctt ttaactgcgg aagaagaagt
    19621 agcgttagca ttgaaaatcg aagaaggcga ccaagaagcg aaacagcgtc tggcagaagc
    19681 aaaccttcgt ttagtagtaa gtatcgctaa acgttatgta ggacgaggca tgcagttcct
    19741 tgacttgatc caagaaggaa acatgggact gatgaaggca gttgaaaaat ttgactaccg
    19801 caaaggattt aagttttcaa cctatgctac atggtggatt cgtcaagcca tcactcgagc
    19861 aatcgctgac caagcacgta cgatccgtat tccagttcat atggttgaaa caattaataa
    19921 attgatacgt atccaacgtc aattgctgca agaccttgga agagaaccta cgccagaaga
    19981 aatcggtgcg gaaatggatc ttccaacaga aaaagtccga gaaatcttga aaatcgcgca
    20041 agaacctgtt tcccttgaaa cgccaatcgg tgaagaagat gactctcact taggtgattt
    20101 catagaagac caggaagcaa caagtccagc agaacacgca gcttatgagt tactaaaaga
    20161 acaattggaa gatgtactag atacattgac tgatcgggaa gagaatgtcc ttcgcttacg
    20221 ttttggttta gatgatggcc ggacgagaac attggaagaa gtagggaaag tatttggtgt
    20281 tactcgggaa cgtattcgtc agatcgaggc taaagcatta agaaaattgc gccatccttc
    20341 tcgttcaaaa caattaaaag atttcttaga ataagcgtgg actattgacc agaagagaaa
    20401 tcagtaaaac tgtttggttt attttaaaag aaaaaaagct tgagactacg cagtctcaag
    20461 cttttttttc tataggaagg aaattcattc attattttta agtaaatcct aaaaataagc
    20521 tattggcgtg acgtatagaa tatgatagat taaatggtag cgtaaaccta gtaaaggagc
    20581 cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg aaaaaaatgg agcagaatac
    20641 tatctgtcca gactgcggtc atcaaataac tgcagaagat cagcaaataa aagaatcaat
    20701 tgaagaaaca aatacagatg cagctatgca ggataaatca actgaagatg cagtctttcc
    20761 tgatacagaa ttaaacgatc caatcgaatg gtcggaattg aaagatttac ctctagaatc
    20821 tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa gaaccacctg ctcacgatca
    20881 agtaactaat atagagaaaa atgaacagaa gaaagcaact gttcatgaga cttcggaaga
    20941 atcaggaaag aatcaagaag aaacgcttga atcaaaacaa aaaagaagag tagctgaact
    21001 gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta tccgcttata tcaaagcaca
    21061 tcgtgaagat acaaaagaag agcacgcgaa agagctgctc aaaatgatca gtgaaaaaat
    21121 tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa actgaaaaag actctgatag
    21181 agaaaacgcc acaggtaaag aaacagtaaa aataaaagac gtaaaaacgg atatcacaga
    21241 aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa aaaacgaata aactgccgga
    21301 agcagagaaa aaagaaatac tcgaaaacac agacaaaaag tcaacagagc ccgatgaaat
    21361 aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa gagggtactg atcttgaagc
    21421 aaaaatagaa aaagaagaga accagcctaa gctgtttgaa gcagaagcga aatcaataac
    21481 aatcgtgcca gatactgaaa aaacacatca agaatcaaac ttagaaaaaa cagatccaga
    21541 taaagcgcaa aagaaaaaat caaaaaaagt accttatctc gttttagcag cagtacttct
    21601 actcggtgga ggaagctggg cttattatga ccatcatcaa aaagtagaag ccgaagtagc
    21661 agcagaagca aaaagacagc aacaaatgga agaacttcat gagaaacttg ctgcctttta
    21721 tacagatgat caccatcaat ttattcgtgc agatatgatt catcaagatt tatcaaaact
    21781 aaaaaatgat ttatccagtc ttaagaatga aaaaggatat tcagagttag aaacaactta
    21841 ccaagatatc caatctaaga tccgagatat ccaaaaagtg aatgagtggt tcatagcacc
    21901 tgccatcgtc gatgatcatc tagcagatga catcaagttg aaagcagata aagagatcca
    21961 agcaatcgaa acggataaca cagattttgg aaaattgatt caacaagcac aaacagaagc
    22021 taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa acagctatag tctttaaaga
    22081 cggaaaagta gtggattcag caacacgcga ccaatacaag acggcaaaag aagcagtaga
    22141 aaaagtgaaa aatacagatc tatctgctca gttgaatgaa caattgaaac aagtagataa
    22201 gatgctcact gataaagaga aaaaagaaga agaggaaaag aaaaaagcag aagaagcgaa
    22261 aaaagcagct gaagaacaag caaaacaagc cgctcaagca gcagctgcag ctcaagccgc
    22321 tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg gcatcctcga ccccttcctc
    22381 ttcaaactca gcgaatcagc caatcaaatc tgccagagca agtgatattg ccgatgcatc
    22441 taatccagca tggacgtggg caccaggtgt ccaaagcaaa gtgattgcta catgtatcca
    22501 acgtggatat atcgttgaag gaggttacag attagaaaaa gccagaatcg aaaatgggga
    22561 aggctattat aatctatatg ccacttcaac aaaatctgca ttgatgaaag gcattggaga
    22621 aagtgctttg ccattttaca tcgtaaccat caactgtaaa actggatggt tcggaggaaa
    22681 tgggagcaat taattttagg caaatgaaag aggctgtgag cctgccgttt aacaccgttt
    22741 attcgaaaaa ggggagtatg acacaactgt tgtcatactc cttttttcac ctttccaaac
    22801 caaggctcag ctattcaagt agataacatt atgttatgat agcaaagaaa tgtattaaga
    22861 aaacctttca ctatagaggg agaaaaaaat gaacgattta ttagcaacta tatttacagg
    22921 tattattatt gatgaaaatg aaaaaaacta ctttgtccaa aaaaacggac aaacctttgg
    22981 cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca gttgaaggat tcggttacca
    23041 aaatcaaaaa catgaaaatc ggatcactac acaaattcct aaaagccgta tcggtcatta
    23101 cgcttttgga atcgttactg ctacacgaaa agatttaggt gcgtttgtcg atatcggtct
    23161 tccagataaa gactttgtcg tatctcttga tgaattgccg acaatgacag agctatggcc
    23221 taaaaaaggg gatcgcttga tgatttccct tagagttgat gcaaaagacc gtatttgggg
    23281 cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa tatggaacag aagaaatgaa
    23341 aaacaaagat gtgataggaa cagcataccg cttgaaatta acaggtacgt acgttttaac
    23401 ggaagatttc tatcttgggt ttatccatcc ttctgaacga tataatgaac ctcgtctagg
    23461 cgagcagata aaaggtcgag tgattggtgt ccgaccagac ggtgtactga atctttcatt
    23521 gaagccaaga gcatatgaag ctatatcaga tgatgcacaa atgattttaa ccattttaga
    23581 acgttctgca gatcagcaaa tcaactttac agataaatcg gatccagaag aaatcatgaa
    23641 agcttttggt atcagcaaag gagcatttaa gcgcgcatta ggtaatcttt tgaaacaagg
    23701 attgattaca caagaaaacg gtgtgacaaa actagtcaaa aaatctaatt gagaatccct
    23761 tgcatgtttt tcttaactag cttataataa ttttaattaa catagtgtta tatctttttt
    23821 tatttgtaat tattataaat tgttggggtt gagtaaatgg tcatgaatgc tatttcggcg
    23881 atgaaaaaaa caaaacagca gctacatgaa gcggggttta agctgacacc gcaaagagaa
    23941 gctactctgc ttgtgctttt ggaaaatgag aaagagcatt tatctgcaga agaaatcttc
    24001 tttttagtga aaaaaaagag tccggaaatt ggacttgcta ctgtttatcg aacccttgaa
    24061 atcttgacag aattgaaaat tgtcgataaa gtaagtttta atgatggatt agcccgttat
    24121 gatttgagaa aagaaggcgc caaacatttc catcatcatt tactatgctt gaactgcggc
    24181 acgatcgagg aagtagaaga ggatctttta cttgaagtgg agcaagtagt agaagaacgt
    24241 tatcattttt tagtaaaaga tcatcggcta acgttccatg gaatctgtca ggaatgctat
    24301 gaaaaagaaa
//
LOCUS       DACEDD010000041        24271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEDD010000041 DACEDD010000000
VERSION     DACEDD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.041"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IWO45_001890"
     CDS             <1..572
                     /locus_tag="IWO45_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0333361.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IWO45_001891"
     CDS             576..1217
                     /locus_tag="IWO45_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0333362.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IWO45_001892"
     CDS             1214..2131
                     /locus_tag="IWO45_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0333363.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IWO45_001893"
     CDS             2128..2799
                     /locus_tag="IWO45_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0333364.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IWO45_001894"
     CDS             complement(3340..3828)
                     /locus_tag="IWO45_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0333365.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IWO45_001895"
     CDS             complement(4029..4295)
                     /locus_tag="IWO45_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0333366.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IWO45_001896"
     CDS             complement(4295..4558)
                     /locus_tag="IWO45_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0333367.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IWO45_001897"
     CDS             complement(4551..4823)
                     /locus_tag="IWO45_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333368.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IWO45_001898"
     CDS             5034..5219
                     /locus_tag="IWO45_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0333369.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IWO45_001899"
     CDS             complement(5361..7184)
                     /locus_tag="IWO45_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0333370.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IWO45_001900"
     CDS             complement(7782..10487)
                     /locus_tag="IWO45_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0333371.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IWO45_001901"
     CDS             complement(10793..11740)
                     /locus_tag="IWO45_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0333372.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IWO45_001902"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IWO45_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0333373.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IWO45_001903"
     CDS             complement(12375..12737)
                     /locus_tag="IWO45_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0333374.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IWO45_001904"
     CDS             complement(12748..13869)
                     /locus_tag="IWO45_001904"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0333375.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IWO45_001905"
     CDS             complement(13885..14235)
                     /locus_tag="IWO45_001905"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0333376.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IWO45_001906"
     CDS             complement(14365..15876)
                     /locus_tag="IWO45_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0333377.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IWO45_001907"
     CDS             complement(16198..17550)
                     /locus_tag="IWO45_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0333378.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IWO45_001908"
     CDS             complement(17703..18779)
                     /locus_tag="IWO45_001908"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0333379.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IWO45_001909"
     CDS             18975..20720
                     /locus_tag="IWO45_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0333380.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IWO45_001910"
     CDS             complement(21000..21437)
                     /locus_tag="IWO45_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0333381.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IWO45_001911"
     CDS             complement(21421..22794)
                     /locus_tag="IWO45_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333382.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IWO45_001912"
     CDS             complement(23116..23604)
                     /locus_tag="IWO45_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0333383.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IWO45_001913"
     CDS             complement(23626..24228)
                     /locus_tag="IWO45_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0333384.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DACEDD010000042        23817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEDD010000042 DACEDD010000000
VERSION     DACEDD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.042"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..779)
                     /locus_tag="IWO45_001914"
     CDS             complement(45..779)
                     /locus_tag="IWO45_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0333385.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IWO45_001915"
     CDS             1106..1516
                     /locus_tag="IWO45_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0333386.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IWO45_001916"
     CDS             1509..2210
                     /locus_tag="IWO45_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0333387.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IWO45_001917"
     CDS             2210..3796
                     /locus_tag="IWO45_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0333388.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IWO45_001918"
     CDS             3815..4036
                     /locus_tag="IWO45_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0333389.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IWO45_001919"
     CDS             4033..4794
                     /locus_tag="IWO45_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0333390.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IWO45_001920"
     CDS             5174..5683
                     /locus_tag="IWO45_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0333391.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IWO45_001921"
     CDS             complement(5758..6297)
                     /locus_tag="IWO45_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0333392.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWO45_001922"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWO45_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0333393.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IWO45_001923"
     CDS             7364..8161
                     /locus_tag="IWO45_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0333394.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IWO45_001924"
     CDS             8189..8824
                     /locus_tag="IWO45_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0333395.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IWO45_001925"
     CDS             8844..9617
                     /locus_tag="IWO45_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0333396.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IWO45_001926"
     CDS             10025..10822
                     /locus_tag="IWO45_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0333397.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IWO45_001927"
     CDS             10800..11213
                     /locus_tag="IWO45_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333398.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IWO45_001928"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IWO45_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0333399.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IWO45_001929"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IWO45_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0333400.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IWO45_001930"
     CDS             15990..16550
                     /locus_tag="IWO45_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0333401.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWO45_001931"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWO45_001931"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0333402.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IWO45_001932"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IWO45_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0333403.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IWO45_001933"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IWO45_001933"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0333404.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IWO45_001934"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IWO45_001934"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0333405.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IWO45_001935"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IWO45_001935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0333406.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IWO45_001936"
     CDS             21897..22475
                     /locus_tag="IWO45_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0333407.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IWO45_001937"
     CDS             22527..23009
                     /locus_tag="IWO45_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0333408.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IWO45_001938"
     CDS             23126..23500
                     /locus_tag="IWO45_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333409.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7774 a         4284 c         5115 g         6644 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaag
//
LOCUS       DACEDD010000043        23758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEDD010000043 DACEDD010000000
VERSION     DACEDD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.043"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(90..395)
                     /locus_tag="IWO45_001939"
     CDS             complement(90..395)
                     /locus_tag="IWO45_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0333410.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(421..852)
                     /gene="ruvX"
                     /locus_tag="IWO45_001940"
     CDS             complement(421..852)
                     /gene="ruvX"
                     /locus_tag="IWO45_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0333411.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(849..1115)
                     /locus_tag="IWO45_001941"
     CDS             complement(849..1115)
                     /locus_tag="IWO45_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0333412.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1369..2094)
                     /locus_tag="IWO45_001942"
     CDS             complement(1369..2094)
                     /locus_tag="IWO45_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0333413.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2460..2530)
                     /locus_tag="IWO45_001943"
     tRNA            complement(2460..2530)
                     /locus_tag="IWO45_001943"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2496..2498),aa:Gly,seq:ccc)
     gene            2647..3399
                     /locus_tag="IWO45_001944"
     CDS             2647..3399
                     /locus_tag="IWO45_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0333414.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3415..3894
                     /locus_tag="IWO45_001945"
     CDS             3415..3894
                     /locus_tag="IWO45_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0333415.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4145..5725)
                     /locus_tag="IWO45_001946"
     CDS             complement(4145..5725)
                     /locus_tag="IWO45_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0333416.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5718..6461)
                     /locus_tag="IWO45_001947"
     CDS             complement(5718..6461)
                     /locus_tag="IWO45_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333417.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6637..7413)
                     /locus_tag="IWO45_001948"
     CDS             complement(6637..7413)
                     /locus_tag="IWO45_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0333418.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7661..8497)
                     /locus_tag="IWO45_001949"
     CDS             complement(7661..8497)
                     /locus_tag="IWO45_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0333419.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8508..9437)
                     /locus_tag="IWO45_001950"
     CDS             complement(8508..9437)
                     /locus_tag="IWO45_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0333420.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9629..9982
                     /locus_tag="IWO45_001951"
     CDS             9629..9982
                     /locus_tag="IWO45_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0333421.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10056..10712)
                     /locus_tag="IWO45_001952"
     CDS             complement(10056..10712)
                     /locus_tag="IWO45_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0333422.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10938..12872
                     /locus_tag="IWO45_001953"
     CDS             10938..12872
                     /locus_tag="IWO45_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0333423.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12958..14181)
                     /locus_tag="IWO45_001954"
     CDS             complement(12958..14181)
                     /locus_tag="IWO45_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0333424.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14175..16073)
                     /locus_tag="IWO45_001955"
     CDS             complement(14175..16073)
                     /locus_tag="IWO45_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0333425.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16067..17185)
                     /locus_tag="IWO45_001956"
     CDS             complement(16067..17185)
                     /locus_tag="IWO45_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0333426.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17268..18101)
                     /locus_tag="IWO45_001957"
     CDS             complement(17268..18101)
                     /locus_tag="IWO45_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0333427.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18094..19011)
                     /locus_tag="IWO45_001958"
     CDS             complement(18094..19011)
                     /locus_tag="IWO45_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0333428.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19034..19213)
                     /locus_tag="IWO45_001959"
     CDS             complement(19034..19213)
                     /locus_tag="IWO45_001959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333429.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(19238..20362)
                     /locus_tag="IWO45_001960"
     CDS             complement(19238..20362)
                     /locus_tag="IWO45_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0333430.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20545..21453
                     /locus_tag="IWO45_001961"
     CDS             20545..21453
                     /locus_tag="IWO45_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333431.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21594..23207
                     /locus_tag="IWO45_001962"
     CDS             21594..23207
                     /locus_tag="IWO45_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0333432.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7405 a         4662 c         3966 g         7725 t
ORIGIN      
        1 tctagcggaa cgtattcaat ctcgtattca attcaaaata caaaaaagct gaacccttat
       61 ctaccaaggg ttcagctacc atttaaagct tattcttctt cttccgccat gaatgtattg
      121 aatacttctt cgatcatatc ccattctgcg tctgtttcga tttgttctaa ctcgccttct
      181 gttccatctt cgttttcgat gtatgcataa gcttgtaatt ctacatcttc atcttctgaa
      241 acaccagctg gatataaaag aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt
      301 agcaagattt cgtataatgt ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt
      361 ccttcgtgtt cgtgggaatg gttatgttct gtcataagat tacctctttt ctttttatta
      421 ttaaaataat tgcttacctg agccgtctaa ataattttgt aaaatcatca cagctgctaa
      481 tttatcgatg actttttttc gtttcgctct tgaagtattt gcctgctcta ctagcatacg
      541 ttctgcctga acagtggtta gtcgttcatc ttgataatca accggcaagg agaatagttc
      601 tttgatcttc tctccatatg acatggaagc ttctgccctt ggtccgatgg aattgttcat
      661 attcttaggt aatccaacta caaaacggtc tacttcgtac tgcgccacta attccttcaa
      721 ccgatcaaaa ccaaactctt cttctgcctc attgattcga atgatttcta ctccttgagc
      781 tgtccagcca aagggatcgc tcacagcaac gcctactgtc ttagaaccga catccaagcc
      841 cattactctc ataaattcac tccatgattt gagaggtagt ctttgacaat tacttccatg
      901 atttcatcgc gttcgtgacg gcgaatcaaa tttcgtgcat cacgataacg aggaatgtag
      961 gccgggtctc cagaaagcaa gtagccgaca atctgattga ttggattgta gcctttttct
     1021 tccaaagccc gataaacaat cgctaatgtt tcgctcactt cttttttgcg actatcgtca
     1081 aaaccaaaac gtacggtttc atctgtaaaa cccatgattc tacacctctt tcccgcaagg
     1141 taattccagc ttaccttcta cacccttatt ttactcgaag aaagggaaaa ctacaaacaa
     1201 atattccaaa cttctgaata aattaagaaa cacgatttgt ctgatttttc cttttttctg
     1261 tggctttttc aaaagccctc tttccataaa gtatacgcta tatttctttg aaagtatgtt
     1321 tttacacaat tttttcataa agttcataaa aaattcgaat tgctgctact aatttttcgt
     1381 tttcgttgct tgccctgtct cataattgat ggtgtagcca tcttgaacat tgtagataaa
     1441 gacattgaat tcaatttttt tgtcttcaat gctttctgct tccaactgca cacctcttgc
     1501 taccagttca tttccctcga aatgaggagt gacacgatag cgtacatgat gatttgtttc
     1561 cttgatatat gccgcaatat cattttcgtg ggcaagcatg tacggagcat tcaacgaacg
     1621 tgtccctgtc attaagtttt taatgttatt attttgacca gtcaactgga aaccaatcaa
     1681 atggctgcgg ttgtataacc attgtccgtc acttaatttc ttttgtttcc aacctgtcgg
     1741 atcaatatac aaaggttctc tcttttcagt aggaaacaag tcttttccca acatcgcatt
     1801 ggcttgtcct actctgttca atttgtctat atttgaatag ctttcccatg tccctttgga
     1861 aagactaaga tcctcttttg taaactccgg ctgattccca ttgatttcta tttcttgatg
     1921 ttttccatca tattctggca catccgctac ttgagaagct tgcggtgtgg aatcaaaaat
     1981 ggttgtaatt tgttggaaga tatctgaatc tttttgtgtg gtggaaatac cgagtgcccc
     2041 tgcaatcaca gcagcgacga tccccacgat cgccttggtt gtttttttgc tcatttcttt
     2101 actcctttac taacagttcc ctaaatctta acatatgttc ccctataaat aaatactgga
     2161 aagaaataga aacagtaaaa aaataaaacc agtttttaga cggcttaaga atacaaaaaa
     2221 agagacggat tcatcgtctc tactcaatca caaaacacta tttacaaggt atttattatc
     2281 attcaaaaaa tttttcttaa tactcaaaac taaacggctt ctgtacgacc tcttggtaaa
     2341 cagtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagtcaaaaa cataagaagc
     2401 ttttttgctt gactattctt attacttgat gcagggcagc tttttcacag cctcttttaa
     2461 gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa gctgggattt taccactaaa
     2521 ctacacccgc gatacaaaaa cagtatacgc ctctttcact ccttcctcaa gtttttttat
     2581 tctttacgct tccgatatgt tttgtagtaa aataaaacta atcaatcaat tattggagaa
     2641 gatgctatga cgggaaaaga attagatctg ttgctggaca cttgtttact cgctgggaaa
     2701 atcatgatgg aaagcaatgc ggagatgtat cgggtagaag atacgatgag tcgaattgct
     2761 ttagcttctg gaaatttccg tttggtcagc tatgtcactc aaaccggtct ctttattggt
     2821 cttgatcgta cctcgaccat ccgaatggaa caaattacca accgttcgat taatttagaa
     2881 aaagtagtaa aagtaaatga tctttcacgt aaatatgttg cttcagagct aacattagaa
     2941 gaactttact gcgaactgca agcaatcgac cgagaccgtt ctttctttcc tgtctggttg
     3001 caaatcgtta gcgcggcagt gatcagtgga acaattatgg tgttatttgg cggaaagatg
     3061 accgatttac ctgtgacttt agtcattgga ggattgggat acgcagctta tctatatagt
     3121 ttgaaatttt ttagaatcaa atttttatcg gaatttttat cttctttatt gattggcata
     3181 gccgctatct acagcgtacg ttttggctta ggaagcaatc aggatctaat cattatcgga
     3241 tgtgtgatgc ctttagttcc tggagttcaa attacgaacg ctatccgtga tttgctagct
     3301 ggacattatg tctcaggagt ttctcgcgga acagaagcga tgatgactgc tacgatgatc
     3361 ggttttgcga ttgcttttat tttccagctt ttttattagg aaaggaacac aagtatgggg
     3421 acgatattta ttcagttttc ttttagtttt ttagcgactg cggcatttgc cgttattaca
     3481 aatgtaccta ggcgttcatt gatttgttgt ggtctttctg gtgcttttgg ctggatgatc
     3541 tactggatct gtgttcaaat aggcggatct gctgcttttg gttcattgct tggttcttta
     3601 ggagtagcat tcatcagcga tcttttctct aaacgtttga aaatgcccgt aacgattttc
     3661 aatattcccg ggatggttcc tctagtccct ggtggtttag cttatcaagc ggttcgaaat
     3721 ctcgttactg aatcctatcg agaagcggct cattatgcgg tccaagcaat tatgattgct
     3781 ggagcaatcg ctttaggtct tgtattatca gaagtcttta accataacat ccgtaatttc
     3841 aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac ggctttttaa ataaacgaag
     3901 tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg agcgatgaca aaatatctca
     3961 caaacattgc gacaagtaac cgcaggagca ataagaagta attatgagta ttaaaaaaag
     4021 ttttgtaaaa atagcttctc acatttttac ataaaattga cttaatactg agaaactgac
     4081 ttttgaacac cctctatctg taaaaaaggg gctgcgacat gatttttgtc acggcccctc
     4141 ttctttattc aaattttttc caaaacgttc gttgataata aacgatccat agagcggaaa
     4201 gtattagaac taaaccgatc acaggaatat tcactaacca aaatggataa aacaaagcca
     4261 acattccgta taagacgata agaataatac tgatgatcaa tgaacccatc atagttgcga
     4321 ctaaacccaa acttcctgaa cctctgttaa ataattggtt aatattggtc cagttcagca
     4381 gcagcaaccg ataatctcta gcgaaataac gtagtgacaa taagtagcag ccgaataaag
     4441 aaccaaatag caagctaggg ataaaaaaca aaggcagctg aaaagacagt ccgcttaata
     4501 atgcgatccc accagttaaa aaactctgca atatccatcc gaatctgaac ttttctttta
     4561 aatattggtt catagagatt ggcaaagaac ggataaacag aaaattttct tgatccaaag
     4621 aaataagatt gctgataaaa gaagtctgat taactgttaa gaaagccaat gcgatcccta
     4681 ccaggaaaac aacaccgatt aacttattat ctaaatcagt taattttatt tcgcctccta
     4741 gggcaaaagc aagaataaaa atgattggag ttaacaagga agaagaaaga acttgcataa
     4801 tcaaattagg ttcttttact aattgagcat tgtaactaaa taaaagctga tgcacatttt
     4861 ggttcttttt atgggttctt tttattttga actcactgtt tgaatcagtc agttgctctg
     4921 taagctttgg tactatcagc tgttttacag caaggaaaaa tcccagagcc gtgatagcta
     4981 gtattcccca acccaataaa ccttgttgag aaagtggctg actggcagca tcatagaatg
     5041 gcaataatat cgaaatcgga tgccgatctg ttaactcatt ttcaatagtc cccgtttgat
     5101 gattcatcca catgattcca gcaaatgcga tcagcatcga gctgccaagc aagatactag
     5161 aaactgtttt tctatgtttt ttaaacactg ctgttcgagc caaaccaaaa acaatcaaac
     5221 tagccagaca aaaaagaata atcaaaaata gagaaaacaa aaggattgag ataatgatag
     5281 taagcaaaat ccctagatca gctcgccatc cggttaaaat aaacatgact agaagcggaa
     5341 aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt tgctgaaaaa acctgttctt
     5401 gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc ttcaaaaaag atattataaa
     5461 tgactgatat actttgagtt aaacctaaca taccaaaaag tgccacgtaa taagtaaaga
     5521 aacctggcaa tttaacaaag ttcatagcaa acatagcaaa tccgtaaatc agtaaaaaaa
     5581 tcagccctga aaaaagattt tgagacaaaa gatagatagt taatcgcttt cccttttttc
     5641 ctttctctct tgctttattc gtgagctgtg gattaacata taaaagatta actcgaatta
     5701 attctaataa ttgtcgctta tacatacggt ccatctcctg tcgcttgcct tccagccatt
     5761 ttcaaataaa tcttttctag cgtttcatca ggtgagccat tcaataattc actaactgcc
     5821 ccctgataaa tcaattcacc tttcttcaaa atagccagtt tatcgcataa ctgttgtgcg
     5881 gtatccaata catgtgtaga aaaaatgact gtttttcctt ttgcggcatg agccttcatc
     5941 atttctttca aatcaaatgc tgcttgagga tcaagtcctt gcaaaggttc atccaatacc
     6001 caaatatctg gatctggcag caacgtgccg atcaaaatcg ttttttgacg cattccatgt
     6061 gaaaaacttg cgattgtttc attttgatga ctatacatat caaaaagcgc tgtcaattct
     6121 gctaaacgct tttctttttt ttgtgtgtct aattcatagg cagctgaaat caagtcccaa
     6181 tattctccag ccgtcaattg taaaaaaata tctggggaat caggcacata accgattttt
     6241 tgtttgatac tcaatcgatt ttccgtcaat ttcattccat ctactaaaat cgttcccgat
     6301 gatggttcaa taatactaac taaactttta atcgtagttg attttccggc accattatgt
     6361 cctaaaaatc caaaaatttc cccttgtctt atcgagagag acaagtcttt taatgcttgt
     6421 tttttcccgt aacttttact tacattgatg aattcaatca tttctgtact ccttttgttt
     6481 gttattactt tttattgtac ccttcgttat tggtggagta aatgtcaaaa agagtgcata
     6541 acaaaaaagg atagaaacga ctaggctgct acttgggttg ttctcttcta caggaaaaca
     6601 ctcttctaac aggtaagata tgagtctttg ttttctttaa aatgattcat tttctatctt
     6661 ttcttgcata aaattcttta gtttatcaat acttctcttt cccatctcta gttgttctcc
     6721 atttgcaaat gttactattt cgtttactct gctaatagat gtaatgtttt cagtattaat
     6781 gatataagct tttaaatctt tacataaata ctcatattcg agtgattttt tatatttttt
     6841 aattgtgccg tttacgagaa tctcttcatc tttcaatact aggcctattt tatttttata
     6901 acctggcatg ctcattagat aaaggatttc atcgtaatgg atatagtgat cgctattacc
     6961 ataacggaca gttgtccacc tttttttcgt agcatgttga acaggatagt tttctttttc
     7021 tttcaataag taactttgga tgcttttttt atttttttca taagtgaatt cttttatcaa
     7081 ataaccacta ggcgtaatct ttcgattaat aacagttatt gctttattcg gatgagacgt
     7141 tagataaaca attttacact tttcattctt ttctctaagc atttcgccta gttcgatacc
     7201 gctataatag gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat agatttcatt
     7261 atttttaatg aattcaacaa attgtataac gttcaatccc tgaacgatct caaatttcca
     7321 aggacttaat aaaattctgg attcttccaa aatttctact agcatttttt caaaaaccaa
     7381 actatcatcc aaaataaata tacgcgttac cataaaaagt acctccactc gatattttac
     7441 tatcaaaatc atttcataca acttttcaac atacatgtta ggaataaatt gtaattctct
     7501 tcacaatctt ttccaacacc tttgaaaccg atatcaaata ctttcaggat gctgcttcta
     7561 aaataaatat caaacaaatg aatacatcat gcaaaaaaag ccagacaacc gacgtccttg
     7621 cgtgtcagct gtctgagcta tgtttattta ttttgttctt ttaaaaagaa cgatacacat
     7681 actgattttt cggagcagag actttctcag cgtttgtaac aactacagat gggatttcac
     7741 gcttgaatgg ttgataatag tctgattgga tggttccttc tattttatac cattcatcat
     7801 ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc aaagactcct gaatctgcca
     7861 cacaatggat aatgccgaat cggaacaaga agatatctgt attgctatcg ttcggtgttt
     7921 ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa ttcgctaggg taattataaa
     7981 ttagctccat aacttctaaa tagttttcat ctgtgatctt gatagtttgc ttatccccat
     8041 acttttcttt caactcattc atttggtcta agtagtctga tttgttgaaa taaatactgg
     8101 tatccggctt taagtattgt gtctgcatct ccggatcccc tactgattct ttacttactg
     8161 ggaaattaaa tcctttggct tctacgatcg tagtgtcgag actaactgtt ggaaacaacg
     8221 tccccacgat tactggcaaa gctagcagta cataagcgat tccacgttga taaggttttg
     8281 acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc
     8341 acaaatacag ctgaactaaa gccaaaagag cagataaaat cattgaaaga atagctaagt
     8401 agcgataatg gacattgata tattgatcga gtttgccaga aacttgcaag tacatcatca
     8461 attcgaaata cccagataaa atcaaaaacc gcagcatatt ttccctccta aataaataat
     8521 gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta
     8581 aaatatcgtt tcatcatcag tagatttttg atatcgacca ttgggccaaa gaccaaaaag
     8641 gcagctacag gtgctgtgcc gaacagactt aacaaagaag atcctataaa cgcatctgct
     8701 tctgagcaaa gagacatcgt aaaggccaat aacagcatga tgataatggc taaaatcttt
     8761 gttgagccga gcgttagcat aattctagtt ggtaaataag tctgcatcgc agctgcgatt
     8821 aatccgccaa tgatcaaata acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac
     8881 acatgcccag tttgatggat cagctgattt tttggttcat tcttctcact ttgttcttct
     8941 tcatggacat gatgatggtg attttctgct actttagacg tgattttttc tttcaataca
     9001 gcgtcttttt gaaaataggc aagccagaca ccaatcacaa gggcaacaaa aaagctgcct
     9061 atcacccgcc agcttgccat ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc
     9121 acgattggat tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg aactggtacg
     9181 ccttttttaa caaattgatg aacgatcggt acgatgccgc attcacatga aggaaagaaa
     9241 aatcctaaga tcgaaccagt aaaaatagag aagatacgat tttcaggaag ccatctcttc
     9301 acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc taataatacg
     9361 aaaggtaatg cttcaataac gatcgataag aaaatcgtac tcatctgcaa aaccgaattt
     9421 ggaataaaag aaaacatgag tttcctcctt tttatttcta gttcattcgc tttgcaaata
     9481 tacgctttta ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact cttatttttt
     9541 accaaagcac ttcaattgac tttttctgtt attcccatta aattaaggtc tcatacatat
     9601 cacttctatc ataaaaggga gaataaaaat gaaacaaacc actaacttga ctgaagttca
     9661 agacatactg caacagtcat ccgttgccgt catttatctt tcgatgccta attgcagtgt
     9721 ttgtcacgct gttcgtccac gactagagga acttctcaca cattatgata ttccggcact
     9781 tcatttggat gcatttgaaa caccagaagt agctagtaaa tttgaagtgc tgactgcgcc
     9841 agttgtcctg atttttcatc aaggaaaaga agtctttcgg caagcacggt tcatcgactt
     9901 taaaaaaatc cgttatctac tagatgaact gacagcagaa gatgattccc tttcttatga
     9961 agaaatcttc cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa
    10021 agttttgcct tcatttgttc aatcgctttt tatttttatt ctgctgtatt ttcggaatgc
    10081 gcatttgcgt cagaatgcaa gaaatagatc aaggtttgca attcattcgt caaatcaaca
    10141 ttttggatca atacatctga cggagcattc aatcgagcag cggtaaagtt caagattcct
    10201 ttgattcctg cttccgttaa ctgattgact acttcttgtg ctctttcagc gggtatcgtt
    10261 aaaattgcta cttcgatttg ttggatcttg atttgttctg acatatcttc cattggataa
    10321 acggggatac cgtctacgat gcgcccaacg atttcctcat tcacatcaaa agcacaactg
    10381 atacgtatgc tattactttg atggaatttg tattttagta aagcacttcc caagttccct
    10441 acaccgatca gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc
    10501 ataagatctt ctacatcata accataccca cgttttccta attcgccaaa ataagaaaaa
    10561 tctcgacgga tcgttgcact atctacttga acggcttcac ttaattcagt tgaagacact
    10621 tttttctttc cagcattatt taaaatcctt aaatagcgat aatacaaagg cagtctttta
    10681 gctgtcgcct ttggaataat atgatctttc acgaaatgac ctccacgtat tgtctaatta
    10741 catctttgat ttctccatca tagcagtatt tataggaaaa aatcaattcc attgctcaga
    10801 tattgtgaaa ttttattctt tattcattta tttatcattt ctgcaaatct cgcagtataa
    10861 tggaatttat ggtagactac taattgaaaa atagtttttt atttgaggag aattcacaaa
    10921 gtagaaaaga ggattccatg atattattac aagccaatca gattgctcgc cattttggtg
    10981 cagaagtctt atttgaaaac atacatttag aaatcagtac aggcgctcgt gtcgctttag
    11041 ttggcagaaa tggcgctgga aaatcgactt tattaaagat cattgctgga attgaagcac
    11101 ctgatgaagg aaagatcgct aaaaataaaa cagcgactct cggatacctc gctcaggaca
    11161 caggacttga ttccaatgaa acagtttgga acgagatgct gaaagctttt gaacaagtta
    11221 gacaaatgga agagcggatg cgcgagctag agattttcat tagcgaaggt acgccagaca
    11281 cacctgctta tagttcttat ttaaaagaat acgaccggct gcaacacgat ttttcagatc
    11341 gaaacggcta tggttatgaa aatgaaattc gttccgtatt gcatgggttt aaattcgatt
    11401 cttcatttta tgatcagaaa atcgatactt tatcaggcgg acagcgcaca cgtttagctt
    11461 tagctcgcat gcttttgcaa caccctgaca ttttgattct agacgaacca acgaaccatt
    11521 tagatatcga gacactttcc tggcttgaga attatctgca aagctatact ggtgcattat
    11581 tgattgtttc ccatgaccgt tattttttag ataaagtagt caatgaagtc tatgaattaa
    11641 gcagaagaaa aatgcgccat tacaaaggta actattcgaa atatctagat ttgaaagcag
    11701 aacagttagc aagtgaatgg aaggcttatg aaaagcagca aacagaaatc cataaattag
    11761 aagacttcgt tgctcggaac cttgttcgcg cctctaccac aaaacgagcg caaagccgca
    11821 gaaagacatt agaaaaaatg gaccggctgg atcgtcctca aggggatgaa aaacaagcaa
    11881 actttctatt cagtatagaa aagccttctg gaaatgtagt cttgcaaatc gaagatgccg
    11941 ctatcggata tgataatgtc accttatctg aaccaatcaa tctagatatc cgtcgtcaag
    12001 aagcgattgc tctagtaggt ccgaatggta tcgggaaatc gacgttgctg aaatcaatca
    12061 ttcaccagat cccttttatc cgtggaaaag aaacgctagg gacaaatgtc accatcggct
    12121 attacgatca aggtcaagct gatctgcata caaacaagac gatcctagca gaattgtggg
    12181 atgagcatcc taccactcca gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt
    12241 ctggcgaaga tgtagaaaaa ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg
    12301 ctttagccaa gttagcgatg aacaaagaaa attttttgat tttggatgaa ccaacaaacc
    12361 atttagatat tgacaacaaa gaagtattag aaaatgcatt gatcgattat gacggcacac
    12421 tgctttttgt ttcccatgac cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac
    12481 tctctgaaaa tggcagcaaa gtctatctag gagactatga ttattattta gagaaaaagc
    12541 aggaagaaga agaaatcgcc gaattgttgg cacaagatga gccagaatct gctcctgctc
    12601 ccacgaacaa atctgattat taccaaagca aagaacagca aaagcttatc cgtagccttc
    12661 aacgaaaaat tactcaggtt gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt
    12721 tggaacaaga aatggtcgcg ccagaaaacc tggaagatca cgtaaaactc aatgagctga
    12781 accaagattt agaagctgct agacaagatc aagaaacaaa attgacggaa tgggaagaac
    12841 taagcttgga attagaagaa tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat
    12901 gctgttttgg tttttctttt tttattttat caaacaaaaa ttccatattt acctttttca
    12961 ccaataagaa gaccaattac tagtcattct cactaaagct tctaaataat agtaatcacc
    13021 ccaaatacaa ggtgtattta ccccattatt tttgggctta ttataaacag actgtgttaa
    13081 aaagccatct tcttcttccg ttgtagcata tctttctttc aattgtcgca taattttcca
    13141 aacagcagac tggtataagt tcttttctgt tttgtctaat agacctgatt ctattaattc
    13201 taataatcca caaaccatga tcaccatact agatgtatct ttttcttctc tgctagaatc
    13261 atcaaagatc aaatcccaat aacaaatggt atcttctggc aaccgattca taaaataatt
    13321 ggctaatatt ttgcttaaat caaaatattt ttgttctttc aaataacgat aacttaatgc
    13381 aaaaccgtaa atcccccatg cttggcctct agcccaacag gaattatcac tataaccttg
    13441 atgcgtactt ccatatcttg gttttccatc tttgacatcc atataaaatg tatgataagt
    13501 tgaaccatct tctcttacta aataatctgc tgcattttga atatgagcct gagcagcttt
    13561 tttatatttt tgatttccag tttcttcact cgcccaatac aataatggaa gattcatgca
    13621 acaatccatg atcattctac cttgttgtga aggatcacta ggatctcccc aagcttgtat
    13681 aactcctgcc ttctcccaaa aacgctgaag taatgtttct gcagcatcaa ttgaagcttc
    13741 cttcattttt cgatttcccg ttattttata atctgctacg gttgataaag aatacaaaaa
    13801 accaagatcg tgatgattag tgataatttg acagtcaatc ctgtggataa aatctatatc
    13861 tgctttttca gctgtttgtt gaaataaagg atcacccgtc ttctcaaata acaaccaatt
    13921 aattcctgtc caaaatcctt ctgtccaacc aatattgtct cttaatggat aacgtccttt
    13981 tataggacac tcgcttggga attgatactg actgttactg atacgtttga ttaacctctc
    14041 cgtgtgtctg cgacaacgct cgatctcatt attccattct ttttccatta attctttata
    14101 acattcattt atccattttt ctggatgtat tatctcttca aacatcactt tatcaagcat
    14161 ttccccacca gcctttagca taattttagt ctttttttat tgtttttatt ttggatagtt
    14221 agcccggttg tcccatcaaa atcaattttg ggacgattgt tccatacttc taaactataa
    14281 tcccgatctt gatgagcaac cactcctgtt ccgagccaat gggttccttt tccaaatgta
    14341 ccctctaatc caggaataaa agtgttttcc catgtcatca agtttgtatt gggcaaaccc
    14401 ataatagctg tggattttcg tttaatagta tcgtcactta aacagataat acctgaaaag
    14461 ccagcttcac tcaccctata gttttccttg tcttcttcta tgattattcc ttctttctca
    14521 ggagaacatt caatagcaaa ccctgtctca tatagaacat attctgtatc taattctact
    14581 ttgtgtacgc gaatctgcca gccttcaaaa ggaataagcc aagttctgat ttttgcctgt
    14641 aaagtaggtt cccatgtaga ggcaagtacc tctttcttac atacaatgtc tcgtacatta
    14701 tttcgaacga tgaactgtcc ataggttttg atgctctcat tcttaaaact ggtcgtgata
    14761 tatgtgtccg ctcgctgaat ccctagcatt gaatccattg ccatttctgc tttcaattga
    14821 ttcgctcgcg gtatcgaaaa accaaattga ctagaataag caaattttaa atatttttca
    14881 ttggtcaatg tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca
    14941 ttatcatgtt gtatgagcaa attggcttct gaaagaagtt tagttccttc acgaataggc
    15001 ataggcattt ctttagccgt ccaatactca tggtcttcag gtagcgccaa caataaaaaa
    15061 atcttattca gccataatgg agataacgta ctattatagg gttcagtcaa tgtcaattga
    15121 gggtaggtat aaccaatatt caggatacct ttttcatcaa atatttcttt ttgcatccac
    15181 catcttatat tccgattgac taaccccttg atttcaccta atttaaaagg caaaagatcg
    15241 gcccacaaca tagctgacca aaagcttgaa actgcaaaac gataaatcat gcttcgacca
    15301 aaaggcacat ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga aaaatcaata
    15361 gctcggttct tatatatctg gctaatgggc ttttctggtt ctagtacgct ataaatcaaa
    15421 ccataataat gaaatgcaaa aggattataa taatcttggc gacctcttga actgtctcga
    15481 taccagccat tttgaagata acaattttct atattatcaa atgcttcttt cagtgctgat
    15541 tctttaactg gataaccaaa tttttggata attgttccga caattatctt aaaaaattgc
    15601 caattaccat ctgcacattc atattcaaaa atttgcgaga accaatgaat aatctggctt
    15661 ttttctcctg tatctaattt tttccataat aattgttcat gatgatataa cgctaataac
    15721 attgcgggca tttctaccat acgctggtct ctttcatgta tttctcccca ataatcggga
    15781 tgggaaggat ctgttccagc ttttattcct gatagaatta atttcaacca tcgttcatct
    15841 aattggtcaa ttgcgggccc cgctccccat aaaacccgag agaatccttc cattcctgca
    15901 attttatttc catatcctac cccttgatgt atataatcaa tttgtgcgcc acttgctcga
    15961 taaaacggct ggagtggttg atttatcttt ttaaataagt caaagtaatc ctgtctggtc
    16021 cttaatagtg gtactggatg tttcattgta ttttctcctc tttttactac caaatagcta
    16081 attccgactg ttctaatcga agtaagcctt caataaaaaa ataatcagaa tagatcagtg
    16141 gcacattata agtcagttct ctatggtatg cttctgctcc gcctccaatg atcccatctt
    16201 catccatatt ccaatttgca tagtgctttt ctgtcttttg gagtatattg atagcagctt
    16261 catgatacat ttttgatcgt acagaggaaa catatcctgc taattccaga agaccgcatg
    16321 cagcgcatac tccagcactg gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag
    16381 gaatataacc agttttttcg attttctgaa taaaatttac tgctatcttt tctgctatag
    16441 ctagatagta cggatttttt gtgtaacgat aacttaacgc gaatccatat aaagcccatg
    16501 catttcctct gctccaacaa gaattctcat tgtatccttg cccgccaagt aaccgaatat
    16561 atttccctga ttctgcatca aattctgcga tatgcccggt tgatccatct ttcctcacta
    16621 gatacttagc tgttgtatct gcatgctgcc gagcaatttt agagaacctc ggatcaccag
    16681 tttccctcga agcccaatac agtaatggta aattcatcat actatcaata atcatccaac
    16741 caggtttatg gtcattccat gctactaaat agttccctga aatattatag cgtccagcaa
    16801 gtaccgtagc tgcatgtaac cctagttgtc gactatgttt gttttgagac tgttgataat
    16861 gtgcaatagc cgatggtaag aataaaaaac ctacatcatg atgaacgtct gaaaaatggt
    16921 acagggcaga ttctaataat tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt
    16981 ttgtcttgtg aaagagctgc cataaaatac cactccaaaa accattggtc caccagtcga
    17041 ttcctctgtc ctttattacg tctgtatatt ttccgttgta agaaatatat ggaattttat
    17101 tccccattct gtctaattcc gcatctatct tttcagaagc tacattcaaa atttttcttg
    17161 accattcctt cgtctgttca ttcatggata tcccgccatt cttttaattt ttatgaatgt
    17221 ggagttgctc ctagaacgaa ctagatggtt aacattcgtt atttttttta cttatttgca
    17281 gctccaatgt cattctcctt aaatttagac tgcgcccaca cataaatcaa gaccattggg
    17341 atcactgaag ttgctaaaat tagcataatt gcgccataat taattggctc acctgtacta
    17401 gaaaccgtca tatttttgat aattaaggga accgtatatt tatctttatc tgaaagcatg
    17461 actaatggaa atagatattg attccaactt cccataaaga taagtaaacc actagtaata
    17521 atcgtaggtg ataaaatcgg tacagcgatt ttcgtaaaaa tcacccactc attggcacca
    17581 tcgattctgg cagcctccat cagtgctgta ggaaattgac tgctatattg tcgcatcata
    17641 aacactgaag ttgtactggc aatcgaaggg atgatcacac ctgcataagt attcgataat
    17701 cctaaacgac tcataatgta aaataaagga atcaatgtgg taaaaaaagg caccatccta
    17761 gatagcataa ctgtattgaa caacagttta ttccccataa aacgatactt tgctaatgca
    17821 taacccgcca ttgtagaact ggctgtagct gaaactgtac tgattacagc aataacaatc
    17881 gaattccaaa taatcaaggg ataattatat ctcgccatga tggcttgcca gttttttaat
    17941 agcatatccc caaacttgaa attgattccg ccacttaaaa tttcttgatt actttgcgtt
    18001 gaagctacaa tcatatataa atatggcatc aaaaatatta aggcaccaaa cacaataatg
    18061 acgtacgtaa aaattctgct ttgtatcttt tgtttagtca tttttatcac ctccgaattt
    18121 aaattgaata gccgataata taacagatac aaacaccatc gtccaggcaa tagcagaggc
    18181 ttttccaaaa tcaaccattt caaaacttgt tttatataaa ttgactccta cgaccaaagc
    18241 tgaattttct ggtccattgg aagtcccaaa gaatatattt gggatattga aattacccaa
    18301 gccattgata gtggctaaaa ctgtggccat taaaatgatt ggctttagca tggggatcgt
    18361 taataaaaag aattgccgaa atttcgatgc tccatctatt tcagcacttt cgtatatctc
    18421 tttagaaatc ccttgaagac cagctaaaaa taaaatagtg ttatatccta cactaagcca
    18481 aatcatcgtg atgatcattg atattcgtat ccaaaaagga tttgttaacc actgtacacc
    18541 gttaattcct agcgcattca aaaatccatt gaataaccca gtcggattga aaaaagtcat
    18601 gaatacggaa gctacagcca ccgttgatgt aatggttgga atataataga cagttctaaa
    18661 aaatcctttt ccttttaact gtgctgaatt caataaaaca gcaaagtaaa tagctaatag
    18721 catcgttatt ggaattgatc ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa
    18781 caatggatct cggaagatag ctagataatt gtcaaatcca acaaattcaa atgagtagcc
    18841 aattttcata aaactaaaag caaacgagat aatgatggga atcaaaatcg tcacaataaa
    18901 gaaaataaca aaaggtgtga ccaatccata agcacttttt ttatgagacg tatctgttgc
    18961 ttttttcttg ctgttcttta ttgtcttgct tggtaacgaa gattgcatca tcgttaagtc
    19021 ctcctaatat tttttaatca ccagttaatt ttacgccgta cttatgagca aaattttctg
    19081 cttcttttag atatgaattt tcaaaatcac ctgtttgcca atagttatat gcagcggtag
    19141 tcaaataaga cgtagcatct gaataagtgg ctggtaaaac agtatttgat gtattttcat
    19201 tgattttatt caatagttca tagtattttt gtcctcctta gtagtcaaac tttttctcag
    19261 ctgcttctgt ttgataggcc aatgagttag atgtagccac gcccagctcc agtgcgttgg
    19321 ctaagcattc ttcatctgtc agcataaatg taagaaattg ttgtgccact tgtggatttt
    19381 tggcatcttt agggacataa aaacttgatc ctcctgatac tgcacctttt cctggatttt
    19441 cttttgaaaa aggaatcagt ggagccatgc gccatttgtc agatgcatca ggaaaattca
    19501 aagacatatt cgtagcatac cagccgccag caacaaacat tgcagattct tgaaatgctt
    19561 tctcagcatc ttgtgcaccg tagaaatgta caatatctga gtcgatcatt tctttgatta
    19621 actttgcagc atgtttcgct tctttagtat tcagacggat atttccttct tcatcaagca
    19681 aagattgatt ctgctgggct agcatgttga taaacaattc ttggtcccca gattctggca
    19741 aggcaattcc aaaactacca gttttttctt ttaatgcttt tgccacctgg atatattctt
    19801 cccaagaact gatttcttca aaatctacac ctatttcagc aaattttttt ggttgataat
    19861 aacaaagtgc tggggtaaaa tcactagccc aaggaataat cgtttcatta ctcgtttgtt
    19921 gacgcaaaat attcaatttt tgatcataaa actgatcgcc atatttgtca taaaatccat
    19981 attcatcagc actataaaaa gcatcttgaa atttttccag cacacctgct gctgaagtgt
    20041 ctgccatgga gataatatcc ggcatatctt cgtgtgttac caattttggg gtgatcattt
    20101 gtactgttgc tgttcccgaa gctacattca caatttttaa gtcaatattt gctccagttt
    20161 tgcggttata gatttttgca gcattttcaa catagtttgt cagtttgccg tttgcgacat
    20221 atacttctaa tgtagtggct ttttttatca ttggatctgc gatgtttgct gttacttctt
    20281 cgttagtttt tccacatgca cttaagctga ataatagggt aatgtttagc aaagctatca
    20341 ccaatttgta tctttttttc atgtctatcc cttctttctt ttcaagttct tatataaaac
    20401 gctttcactt atgtatatta acggaattag taacacaaat atttatataa aaaaatattt
    20461 tacttgtcgt tttggctttt catttttgtt ataatagtca ttaattataa atagaaagct
    20521 ttttatataa aaagagaaag gaaagtggtt cttttgttaa caaacaacca atcctccaat
    20581 cttgtcaaaa aaacactaac ctattccata gatacccatc ttcatttatc tgctgctccc
    20641 tattttcgac tactaataaa tgaaagcgct aatttatatg ctgaatttgc acataaatct
    20701 atagaactag ataatccttc ccttttattc attgataaac agcaagaagt ccaaatcttt
    20761 tctactcaaa aggaaacaca aacttttatc attatccagt tttattttga tgagcaatta
    20821 ttagcacata ttacgggaca atctttttct atagatcaag aaataacatc tatcattact
    20881 tttctgaaaa atgaacagtt caaaattgat tatctaaaac atagtaagcc agctatttcc
    20941 tatgatcagg aaatcaattc ttttttcata ctatgccaac agatgagcat tcatttgctt
    21001 tccttattag ttacttattg tctgcaaaaa attcaattgt cttccttacc aaggcattat
    21061 caacaggaac ttgctttatt gacaatacct tcaaatgaga aagtcattcc taaaaaaaag
    21121 catattcttt attcagatga attagttgat ccaatcatac gatatttaga agaacatctt
    21181 tcagaaaatt ttttgcttga acaattagcc caaatttttt ttatcagtcc agccagctta
    21241 aaaaaacatt ttagaaaagt gaatggaaaa agtgttatta gctattttaa agaactaaaa
    21301 atggaaaaag caaaacgtct cgtgctagaa aatgaattaa cctatacaga aattgcagat
    21361 atattaggat ttcattcgat ccatcatttt tcttcagctt ttaaaaaata tacagggtta
    21421 tccccttcaa agtattatct atccttaaaa tagaaaaaaa tttttaggtt ttatccacaa
    21481 attatttctc tatattttct gtctattcaa aataaacaat aattttccgg cacaattatt
    21541 tgctgttttt taccataaca tagtacgatc aacgtagaaa aggagatgtt tttatgaaag
    21601 ttgttgtcat tggcggtgta gctggcggtc cttcatttgc cactcgtttc cgtcgattga
    21661 atgaagcgca cgaaatcatt atctatgaac gcggagaaaa tatttcttat gcaagttgtg
    21721 ctttgcctta ttatttagga ggtgtgatca ctgaccgtga ctcgctgatc gaacgtacac
    21781 cagaaatatt gaaaacaaaa aacaacatcg atgtctttac taaacacgaa gtaacagcaa
    21841 tcgatccttc tactaagcga ttaacagtta aagacctatc cacaaatgaa gaaataaaaa
    21901 cagattacga taagttgatc atctcttctg gtgctagacc agattatccg gatattcccg
    21961 gagtttttga agcagaaaac ggctttgtcc tccgtagtgt gacggatgcg gatcgaatca
    22021 aatcgttcct tgaagaaaaa aatccacaac atgtcgtcat tcttggtgca ggtgttatgg
    22081 gtctggaatt agctgagaat ttcaagcatc gcggcttaga cgtgacttta atcgatcaat
    22141 tgccacaagt cgctttccct tatgatccag aaattgctaa tttagtttat gacaaattgc
    22201 tgaaagaagg attagccgtt catttagaaa caagagttac tgagatccgt gataaaggtc
    22261 gagaaatagt attatcagat ggctccgtcc tttctgctga tatgctaatt tttgctgttg
    22321 gtgtttctcc gaagaatgaa gtggtgaaag caaccggcat acaattatca gatacaggtc
    22381 agatcattgt tgatgaccag ttacaaacca atctgccgga catctatgcg attggcgata
    22441 ttatcgaaac gactagtgta gtgactggtc agccgatcca aagtatgctt tccagtgcgg
    22501 ccaatcgtca aggacatatg ctggcagata ttttaaatgg tacgcctatg cgctatcgcg
    22561 gatatattgg tgcaggtgtc gcaaaaatct ttgatcatac agcaagttat gcgggaatga
    22621 cagaacatga attaaaagca tcaggcataa caaattataa aactgttttt atcactccct
    22681 ttgaccatgc ctatttctat ccagaagcta caagattaaa tctaaagctg atttttgatg
    22741 cagatagcgg tcgtatttta ggtggacaag catttggaga aaaaggtgtc gataaacgga
    22801 tgggagaact ttctgtagca atcaccggaa acttgacagt ctttgatttg cctgatttgg
    22861 agttgcctta ctctccaccg tattctacta cccgtgaccc gttgaatata gctggttatg
    22921 tcgcaatcaa tcaaatgacg aatatcgtag aaacgatcaa agcaagtgat atacccgaaa
    22981 acgatttgaa agaagcgttc tttttagata tacgtgaacc taataaagca ccttctggca
    23041 gtatctcggc aacaaaaaat attccaatga atgagttacg agatcgaatc aatgaaatcc
    23101 caaaagataa aaaagtttac attactttca gaagaggatt gaatacttat acttctgccc
    23161 gaatcttggc aggtttgggt atcaaagcga ttttgattga agaataaaaa aacgaccgcg
    23221 gtgggtagcc gcggtctagg agttattttt acttgaggag taaaaatgaa aaagtatata
    23281 gggttgttgg ttggtatgta tatagaatag aagataaatg tgaagaaatt gtgcattatt
    23341 tcttcacatt tattaggtta tagtttaaga aagtttaaac attgcgcaat ttaattattt
    23401 tagctgtttc ttttcataaa aaaacatgtt gtgactctga gacaaatcat agtccatagt
    23461 gatgtctcta gagtaactac gcctaatatt acgaggcatc atattaatct atcagcacta
    23521 gtacctaaac tagcatccct ttcgaatatt ttttcttata taataaagtt tgctttcact
    23581 cctctaatct caccaaatcg actggcttgc gttgtgtgat actaggaact gattggttta
    23641 gaacgacttt tctattgtaa tcatattttt cgccatcctc gttgtcttct aatccttcaa
    23701 tgaatgatcc ctaatgtgtg tattaaataa tttaattttt ttgttcctat ttatttaa
//
LOCUS       DACEDD010000044        23572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEDD010000044 DACEDD010000000
VERSION     DACEDD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.044"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IWO45_001963"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IWO45_001963"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0333433.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IWO45_001964"
     CDS             complement(1173..1868)
                     /locus_tag="IWO45_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0333434.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IWO45_001965"
     CDS             complement(1881..2597)
                     /locus_tag="IWO45_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0333435.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="IWO45_001966"
     CDS             2927..3532
                     /locus_tag="IWO45_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0333436.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="IWO45_001967"
     CDS             3857..4180
                     /locus_tag="IWO45_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0333437.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="IWO45_001968"
     CDS             4362..5732
                     /locus_tag="IWO45_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0333438.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="IWO45_001969"
     CDS             complement(5862..7163)
                     /locus_tag="IWO45_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0333439.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="IWO45_001970"
     CDS             complement(7393..9384)
                     /locus_tag="IWO45_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0333440.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="IWO45_001971"
     CDS             complement(9528..9794)
                     /locus_tag="IWO45_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0333441.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="IWO45_001972"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="IWO45_001972"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0333442.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="IWO45_001973"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="IWO45_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0333443.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="IWO45_001974"
     CDS             13138..13701
                     /locus_tag="IWO45_001974"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0333444.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="IWO45_001975"
     CDS             complement(13954..14475)
                     /locus_tag="IWO45_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0333445.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="IWO45_001976"
     CDS             complement(14478..15320)
                     /locus_tag="IWO45_001976"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0333446.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="IWO45_001977"
     CDS             complement(15313..16038)
                     /locus_tag="IWO45_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0333447.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="IWO45_001978"
     CDS             16214..16510
                     /locus_tag="IWO45_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333448.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="IWO45_001979"
     CDS             17078..18670
                     /locus_tag="IWO45_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0333449.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="IWO45_001980"
     CDS             18683..19423
                     /locus_tag="IWO45_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333450.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19591..20940
                     /locus_tag="IWO45_001981"
     CDS             19591..20940
                     /locus_tag="IWO45_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0333451.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21068..21658)
                     /locus_tag="IWO45_001982"
     CDS             complement(21068..21658)
                     /locus_tag="IWO45_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0333452.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21805..23157
                     /gene="brnQ"
                     /locus_tag="IWO45_001983"
     CDS             21805..23157
                     /gene="brnQ"
                     /locus_tag="IWO45_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0333453.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6751 a         4746 c         4490 g         7585 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaaaat tttggaatct tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa
    19561 ttaaactttt ttagttagga tgatttaaaa atgaatgaag agcaagaact gcagcgtgga
    19621 ctgaaaaata gacatgtcca actgatttct attggcggag cgattggaac ggggttgttt
    19681 ttaggttccg gaaagtctat ccagctagct ggcccttcga tattgctcgc ctatcttatt
    19741 actggatgca tctgtttttt cattatgcgt gcgttaggcg aacttctgtt atcaaacacc
    19801 aataaccatt cttttctgga ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc
    19861 actggctgga cgtattggtt ctgctgggta tcgattgcaa tggccgattt aacagccatc
    19921 ggaatgtatg tacgctactg ggcgcctggt gttcctcagt ggcttcctga actgattgca
    19981 ttaggtcttc ttctttgctt gaatatggtg gctgtctcat tgtttggtga gttagagttt
    20041 tggtttgcat tgatcaaagt tgtagcgatc attgccttta tcctcgtagg agcttatatg
    20101 atcctcaccc actatacgac agatattggc acagccagta tcacgaatct atggtctcat
    20161 ggcggattct ttccaactgg tgcaaaagga tttatcttag ctttccaaat ggtaaccttt
    20221 gcttttgctg ggatagaatt agttggatta gtcgctggag aaacagaaaa tcctgaaaaa
    20281 gtgttgccgg aagctatcaa taatattcct atcaggatca tcttatttta cttaggatct
    20341 ctttttgtca ttatggcaat ctatccatgg aacagcttga atccagataa cagtccattt
    20401 gtagaagtat tttcggaaat tgggatcacg attgctgctt ccttgatcaa tttagtcgta
    20461 ttaagtgcag cagcttctgc ctgcaacagt gcaatatata gtacaggacg tatgcttcgt
    20521 tctttagcgc aagaaggcag tgcccctaaa aaattcaaaa agttaacaac acatcatgtg
    20581 ccagggaatg ctttgacatt ttctactatc gtcattttca tttctgttat cttgaattac
    20641 gtgatgccta gtgaagtatt cacactagta agcagtattg ccactacttg tttcttgttt
    20701 atctggagta tgcttgtcta cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa
    20761 gctcattcat ttaaaatgcc gttgtaccca tttagtaatt atctggtatt tctttacatg
    20821 gcctttgtgt gtgtcgtctt gttcctagga aaagatacga gaatcgcttt attactaaca
    20881 cctgtgtggt tcttgcttct attactaatc tatcatatga aatatgaacg taagaagtaa
    20941 gaaagtagtt gtgacagaag tggtcagctt cgagaaataa gaaggaattt acgaaaattg
    21001 cttcttaaat ttttgtgaaa tttcggctta tttcttggag ataaaagctt ctgtcccagc
    21061 ctcttttcta tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa
    21121 aaatccaaat accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga
    21181 caccggcaat caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca
    21241 aaactctcac gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac
    21301 caaaagaaaa actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt
    21361 cagggttacg cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa
    21421 agatattcga ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat
    21481 ggttcaccca gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc
    21541 ggatgaccca taaagaatga aagctagatg tattagggat cttgatctct aatacaataa
    21601 ctctcaaaac aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga
    21661 tgattcctcc tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccat
    21721 cgttttatat agggaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt
    21781 aaaaaaaccg taaaggagtt atgtatgaca aaaaaattat catttaaaga ctatcttttt
    21841 ataggctcca tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat
    21901 ttgggtcaag aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt
    21961 atcggtcttc ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat
    22021 gaattagcaa aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta
    22081 gttatcggac cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta
    22141 gcaccattta tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt
    22201 tttgtgctgg cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag
    22261 ttcctgaatc ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat
    22321 ccactaggtt ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc
    22381 acagggttta ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc
    22441 atcattgtca caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac
    22501 acgatcaaat ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct
    22561 tatttaggtg ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg
    22621 gctcagattg ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt
    22681 atattagctt gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca
    22741 gatttgtttc cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt
    22801 ttagcggcaa acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc
    22861 atctatccat tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag
    22921 cgtacggctg tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg
    22981 ttgaacgctt gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt
    23041 gcggaaagct acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc
    23101 ggttttgtta tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa
    23161 aagaggttgt gaaagagacg ttcagccttg agcaataaaa agtaattttg aaaaatggct
    23221 tctcacattt ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgttta
    23281 ttgagaggtc gtgaaaaagc cgcttagttt caagtattaa gaaatatttt gtgaaaatgg
    23341 cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt gaacaccgtt
    23401 tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa gtagctccat
    23461 atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac aacatttctt
    23521 ttataatata gattttagac atatatatga tataaaaata gttttcgcaa aa
//
LOCUS       DACEDD010000045        22985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEDD010000045 DACEDD010000000
VERSION     DACEDD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.045"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWO45_001984"
     CDS             complement(109..564)
                     /locus_tag="IWO45_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333454.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWO45_001985"
     CDS             complement(718..1926)
                     /locus_tag="IWO45_001985"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0333455.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWO45_001986"
     CDS             complement(2038..2370)
                     /locus_tag="IWO45_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0333456.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWO45_001987"
     CDS             2518..3393
                     /locus_tag="IWO45_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0333457.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWO45_001988"
     CDS             complement(3475..4056)
                     /locus_tag="IWO45_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0333458.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWO45_001989"
     CDS             complement(4062..5321)
                     /locus_tag="IWO45_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0333459.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWO45_001990"
     CDS             complement(5323..6369)
                     /locus_tag="IWO45_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333460.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWO45_001991"
     CDS             complement(6604..6879)
                     /locus_tag="IWO45_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0333461.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWO45_001992"
     CDS             complement(7122..8432)
                     /locus_tag="IWO45_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0333462.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWO45_001993"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWO45_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0333463.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWO45_001994"
     CDS             complement(9978..10658)
                     /locus_tag="IWO45_001994"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0333464.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWO45_001995"
     CDS             complement(10707..11342)
                     /locus_tag="IWO45_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0333465.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWO45_001996"
     CDS             complement(11408..12811)
                     /locus_tag="IWO45_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0333466.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWO45_001997"
     CDS             complement(12798..13829)
                     /locus_tag="IWO45_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333467.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWO45_001998"
     CDS             13860..14081
                     /locus_tag="IWO45_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0333468.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWO45_001999"
     CDS             complement(14246..14983)
                     /locus_tag="IWO45_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0333469.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWO45_002000"
     CDS             complement(15136..16476)
                     /locus_tag="IWO45_002000"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0333470.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWO45_002001"
     CDS             complement(16713..18236)
                     /locus_tag="IWO45_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0333471.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWO45_002002"
     CDS             complement(18614..19225)
                     /locus_tag="IWO45_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0333472.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWO45_002003"
     CDS             complement(19555..20271)
                     /locus_tag="IWO45_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0333473.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWO45_002004"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWO45_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0333474.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWO45_002005"
     CDS             complement(20830..21627)
                     /locus_tag="IWO45_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0333475.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWO45_002006"
     CDS             complement(21597..21968)
                     /locus_tag="IWO45_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0333476.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWO45_002007"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWO45_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0333477.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6445 a         4912 c         3900 g         7728 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa ttaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggttt
//
LOCUS       DACEDD010000046        21967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEDD010000046 DACEDD010000000
VERSION     DACEDD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.046"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IWO45_002008"
     CDS             <1..376
                     /locus_tag="IWO45_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0333478.1"
                     /translation="INPDFIETVNYVTNFQREMRKVEAMTVLATQSPQEMVPENMSSD
                     NFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKSMTNSELESIPQQNQGDAVINFGS
                     KETIRVHFSPNKRQLKLFDGGK"
     gene            376..1581
                     /locus_tag="IWO45_002009"
     CDS             376..1581
                     /locus_tag="IWO45_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0333479.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            1597..2241
                     /locus_tag="IWO45_002010"
     CDS             1597..2241
                     /locus_tag="IWO45_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333480.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            2252..3058
                     /locus_tag="IWO45_002011"
     CDS             2252..3058
                     /locus_tag="IWO45_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333481.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            3080..3310
                     /locus_tag="IWO45_002012"
     CDS             3080..3310
                     /locus_tag="IWO45_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333482.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3856..4131
                     /locus_tag="IWO45_002013"
     CDS             3856..4131
                     /locus_tag="IWO45_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333483.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            4173..4595
                     /locus_tag="IWO45_002014"
     CDS             4173..4595
                     /locus_tag="IWO45_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333484.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            4614..6206
                     /locus_tag="IWO45_002015"
     CDS             4614..6206
                     /locus_tag="IWO45_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333485.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            6218..6553
                     /locus_tag="IWO45_002016"
     CDS             6218..6553
                     /locus_tag="IWO45_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333486.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            6700..6894
                     /locus_tag="IWO45_002017"
     CDS             6700..6894
                     /locus_tag="IWO45_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333487.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            6926..9298
                     /locus_tag="IWO45_002018"
     CDS             6926..9298
                     /locus_tag="IWO45_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0333488.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            9359..10819
                     /locus_tag="IWO45_002019"
     CDS             9359..10819
                     /locus_tag="IWO45_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0333489.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            10830..13034
                     /gene="topB"
                     /locus_tag="IWO45_002020"
     CDS             10830..13034
                     /gene="topB"
                     /locus_tag="IWO45_002020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0333490.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            13273..13866
                     /locus_tag="IWO45_002021"
     CDS             13273..13866
                     /locus_tag="IWO45_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0333491.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            13882..14781
                     /locus_tag="IWO45_002022"
     CDS             13882..14781
                     /locus_tag="IWO45_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0333492.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            14784..15269
                     /gene="ssb"
                     /locus_tag="IWO45_002023"
     CDS             14784..15269
                     /gene="ssb"
                     /locus_tag="IWO45_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0333493.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            15635..15895
                     /locus_tag="IWO45_002024"
     CDS             15635..15895
                     /locus_tag="IWO45_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333494.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            16336..16542
                     /locus_tag="IWO45_002025"
     CDS             16336..16542
                     /locus_tag="IWO45_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0333495.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            16542..16793
                     /locus_tag="IWO45_002026"
     CDS             16542..16793
                     /locus_tag="IWO45_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333496.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            16808..17245
                     /locus_tag="IWO45_002027"
     CDS             16808..17245
                     /locus_tag="IWO45_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333497.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            17238..17945
                     /locus_tag="IWO45_002028"
     CDS             17238..17945
                     /locus_tag="IWO45_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333498.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            18326..18505
                     /locus_tag="IWO45_002029"
     CDS             18326..18505
                     /locus_tag="IWO45_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333499.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            18846..19109
                     /locus_tag="IWO45_002030"
     CDS             18846..19109
                     /locus_tag="IWO45_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0333500.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            19103..19453
                     /locus_tag="IWO45_002031"
     CDS             19103..19453
                     /locus_tag="IWO45_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0333501.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(19477..20091)
                     /locus_tag="IWO45_002032"
     CDS             complement(19477..20091)
                     /locus_tag="IWO45_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0333502.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            20405..20827
                     /locus_tag="IWO45_002033"
     CDS             20405..20827
                     /locus_tag="IWO45_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333503.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            20837..21040
                     /locus_tag="IWO45_002034"
     CDS             20837..21040
                     /locus_tag="IWO45_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333504.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            21251..21835
                     /locus_tag="IWO45_002035"
     CDS             21251..21835
                     /locus_tag="IWO45_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0333505.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         8171 a         3504 c         4436 g         5856 t
ORIGIN      
        1 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
       61 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
      121 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
      181 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagtgg gtaaatcaat
      241 gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt
      301 tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat taaaattgtt
      361 tgatggagga aaataatgga aaaccaaaat gaaagtttaa tcaagcagta tgttaagcgt
      421 cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
      481 ttgatcactg tgttcgtgac cctatttcta atacttgcgg cgggctcgat tgataattcg
      541 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggcttaccg
      601 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgccgta
      661 ggtgcagcag tgaaatataa attattacca agtgtgatcc tttcgcaata cggttacgaa
      721 tcagcgtttg gtacatcggc gtccgcaaga aatgatctca actattttgg gattacttgg
      781 tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat tgaaggtggc
      841 tggtacatga agtttcccaa ctcaaaagct gcattttcct attacggctt tatggtagcc
      901 acacaatcca attttaatgc ttgtgtagga aataaaagcc ctggcgcaag cttattgatc
      961 ttaggacgag gcggttatgc cgctgcagga attacggaag atagtccgta ttatacaggt
     1021 tgtatgtcaa ttatcacttc taataaatta accgaatatg atgaattcgc catcaaacat
     1081 tggggtgaag gtggcaataa caacggcacg attactggtg aatggacgaa tccattccct
     1141 ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac gaatccaggc
     1201 ggtgaattcc gtccgaacgg cttccatgat ggattagatt ttggttcagt tgatcatcca
     1261 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
     1321 tcaggtttgg gcgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
     1381 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
     1441 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
     1501 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
     1561 ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc
     1621 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctg
     1681 aaagaggaac aagcagcaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
     1741 aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
     1801 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
     1861 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
     1921 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
     1981 gacaaattaa gtcctacaga cgatgatcac caacatgatg attacggaag ttactccttt
     2041 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
     2101 gtcgtatttg ttgattatac aatcggcaat ccgcaattca aagatgtcaa accacaaaaa
     2161 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcatagac
     2221 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag aaagacaacg
     2281 tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc tttatgattc tcggcttttt
     2341 gattcttgct agtccgattt atacgaatca gaaggtagat ataccagatg aggtaagcac
     2401 gaacgccttt ttaaacacga aagagctgac aatggtttca aaagaaaaag gagcgaacca
     2461 gcaattagtt tttgaatttg cgatcgatga tgaagatcaa agcttgttaa aagaactggc
     2521 taatcttaat tatcgcgtag aagtaaagac agctaaaggg gattatcaag caatacgagc
     2581 caaagtgatt aaggtgtctg atgattattt tgcagtgaag ctaacgcatg taccagaaga
     2641 atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag tggatatgca
     2701 agaaccacaa gatctgattt attacgtgca cgaagataag gttaaagatc aagtgaaaga
     2761 tgaagattat gaacaacacg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
     2821 agcagctcaa aaacaaatgg aatctttcca agcaaccatt gatttgaatg aagaattagt
     2881 gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
     2941 caagattaat agctaccagc aagaaattga aacatcgaaa acgcaactga aagatcaaga
     3001 agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa
     3061 ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata ctttcgctga
     3121 gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga
     3181 agtgaattta tctaacacaa caatatctga gttgaagaat ggtcgaaaga atttagatac
     3241 tttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt ttacagctga
     3301 taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat gcttatgaat
     3361 cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac cacgattcta
     3421 acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga agatttcaac
     3481 caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc atttttcata
     3541 tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt ctaaatacac
     3601 aaataacctt aatttttaaa gagcataagg aaacgtgata tcactttctc acgaaaggtg
     3661 ttaggtaggc tgtgcctacc acgtttctaa gaaagataaa tgatatcaaa attttggatt
     3721 tgtgtgtaga tttgtgggta ggatgaaagc gaatgtgggc agtaaaataa gcgtttgtgt
     3781 gtagcaaaat caaaaaatgt gtagggatat gtgtagctac acatatccct acacataata
     3841 aatttggagg aaaatatgga cttattaatt agaaatattg aagaaaagta tattaataaa
     3901 attgacaaac gttgtgaaga acttacggtc aagactggca aaaaatggag tcgaaatcaa
     3961 tacctaaaag ttttgatcga aaatgatttt gatcacgctc ttctgaatta taaaaaagac
     4021 caatttgatc ggttgttaga aaaatttgtg gatattcaaa cttataatac gaaaacttta
     4081 gaagaatata ttgcaacaaa taatcaatta attggtttgt taattgaata ataaaaatag
     4141 tattgtgaat ttataaaagg aggaaatcaa ttttggcaaa agcaacaaga aaacatgtat
     4201 atctttacga tatgcatatc gaagtacttg atgaaataat ttctactcat gaagatatat
     4261 ctaattatag tgaagcagtc agacatttgt gtatgatgta tgatccaaaa atcgaagaaa
     4321 aaataaatcc aattcaaatt aaattgaatg caatgggaaa agaaatttct attttaaccg
     4381 aaatggtttc agagtttgcg gatcttcatt tgaaagatac aggaatacta attggtacaa
     4441 attcaactgt ttatcaagaa gcagaagaac gtgtggaagg aaaaattaaa tccaatcaaa
     4501 ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga atctgttcat accagagtag
     4561 atccatttga tccaacgaag attctaaaaa gataaaagta gaaggagaac gtgatgaata
     4621 aatcgcctgc agttattctt acttgtcaat ttaccttgcc caataaaaaa tctttttcaa
     4681 catacattga ttatatgaca agagaaaaag cactggaaga aatggaacga cgtacgcccg
     4741 aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat attcctgaag
     4801 gagaaacttt ttcggaaagt aagacggata aagaattcag tgaaacgtcg aatgaagctc
     4861 aatcgttgat ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaaat
     4921 atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg
     4981 aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca gagccacata
     5041 aaaaagcttc aggaaatctt cctggagttt tttctatggc ttcagacgaa gtgaaaggaa
     5101 gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca acaaaaaggc tcaatcattt
     5161 atcaagatgt tatctctcac gacaattcgt atcttgaaaa aatgggcttg tacgatccca
     5221 aaacagatac cttagatgaa gaaggtttga aagcagctgg aagaaaaatg atggagacat
     5281 tattcgagaa agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca
     5341 ctaagcatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccgtata
     5401 ctgagattga aaatggtgtg agttatatcg taccgaaggg gaagagaaga caagcaacat
     5461 tagacgcaat gaaaaccaat tatgtacatg agttagaacg tttctcttat gaaaaggatc
     5521 gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa aagtgatttg cgtaacacct
     5581 taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca cgccatgaac ttattaaatg
     5641 aagtatataa aaccctacct gaaaaacgtt cggaatggaa ttatggagat gaaaatcgaa
     5701 ccaagctttc accatttaca aggggaaaac ttgatgagtt aaccaaatat gttttaacaa
     5761 aagaacctgg atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagccaaaa
     5821 gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga aaaaaacgcc ctctttgatt
     5881 taaaaaagcg cacaggaaat gcgattttaa cagaactgaa gagacaggat agtcaaataa
     5941 aaccattaat agaaagtgtc aatcagatgc aatctattta caatgaacga tcctttcgaa
     6001 cactcaaaca gttgccaaca aatgaataca agtatagtta tcaagaattc gtaaagcgta
     6061 ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt gcgtcaatta aatcgaacaa
     6121 ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata tgaagagcaa cagcgaagaa
     6181 tccaaatgga acaagaaaga caataggagg aagagaaatg aaacaatatt tagatcaatg
     6241 gaaagtcata gaaggatcac taaaagaaga acgaatcgaa caacttcctg attgtttaga
     6301 aaaagagcat ttgtttcaga taagcgaaat gttgagaaac gaacagtttg atccaaatca
     6361 atttttagtt gttgagtacc cagctactgg tgtgtattgt tgtaatcatg tgaagggtga
     6421 aaaatatttt attattcaag aatacgaagg taagttagcg ccttattata ctacttggga
     6481 aataaatgaa gagggaatca ataatttccc atgtgaaagc attgaagagt ctatttctct
     6541 cacggaatgt taaataaata gagacgtaaa agagtcctga acgtgattgt ccaggactct
     6601 ttttcaatga attgttttcc gccaattcac tgagtggtgc gattcaattt tattatatca
     6661 aaagaaagtg agattccaaa gtgaagggag gaagaagaaa tgactcagac aacgattggt
     6721 gttgtggttg aaaaaagcag ggataatctc gtttttgtga cagaaattca aacaggtaga
     6781 gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa atggcaatat tttaacgtta
     6841 aatctgacta cgaagatgtt cgttgatgca gtagaaaatt atccatttgt ttagacaaac
     6901 gatattttac aaggaggaaa cggacatggc agaagaaaaa gaaccactag ataatagtcg
     6961 gttgggaaag tccaaaagaa aattagtacg gttgcaaaac gaattaaata atcaatttca
     7021 aaagctattt gatcaccaaa aattgacaaa tgggcaacca atgaatgaca aacgaaatgg
     7081 tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa atacaaagtt tgagagaaga
     7141 gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga caagaagagc taaaggaaat
     7201 gggctataac aagtacggcg gactggatat gacgatcgat aatattccaa gaatcaaaga
     7261 agaaatcgaa cgctttgaaa aaggtgaatc gactttttca gccgccacga tccgaaaata
     7321 tcaacggaag ttggaaacat tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa
     7381 tatcctccct gaagtacaag cgatcattga cagcggacga gtgacgcaat ggaaaaaaaa
     7441 tccaaccatt tattttttga aaggctatcg aaaagttgct ttagaactga atgaaaacgg
     7501 gggatttgag gaatcaaaaa aataccaagc cacgtcggaa gaggaaaaag cagctgttcg
     7561 tgaattactt gaagaggctg caccaactga ttctctgaag gagaaagaaa aagatcattt
     7621 ctatcgagta gaattcaacg aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga
     7681 gatcgtaaca ttagaattga tcgatcaact taaaacgttg gataaccaat acgctgcaga
     7741 agcaggttat tataaattct attttgaaga agtgaagaac ggagaagtag tagatcagat
     7801 ccgaatggat ctaggggatg gcgtcaacag taatcacccc atctatttag agttagcaaa
     7861 attgtgcgaa caagtacctg agaaggaaca aaaacaatcg gtcaaaaatg aacctgtttc
     7921 gaaagaacaa atgcagtcga aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac
     7981 ggcagaagag atcaaacaag aagttaaatc cctttcgcaa caggcactgg atgcggtgaa
     8041 aaagcatacc cattcttctg aagacgtcaa agaacttttg aactttatgt ctcgttttcc
     8101 agaacgatcc tttagaaatc aattattgat tgaggaacaa tttccaggtg caacggcttg
     8161 tttaggtcgt gccgcgttga aaaaagaagg aatctatatc aaaaaagggg aacaagggaa
     8221 caagatcttt gtcagaaaaa cggtcaaagg attttatgag aaaggacgtg gctttgttcg
     8281 agaaacagaa gctacaccag aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat
     8341 caaaaaacct tactacacga ttgaaaaagt gtttgatatc acacaaacac agttaaaacc
     8401 tgaagactat ccgaaaatct ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg
     8461 acagtcagaa cttcaagctg ggattcaagc ggtagccaac aatattggaa tacaaattcg
     8521 agatatgaca gaaagtgaag tctatcaaag agaattagga caagcacgtg gggcgtatgt
     8581 tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat actcgaaata ctcctacgca
     8641 gcatttagct acaagtattc atgaattagc ccatgcaaga atgcacaaat tttcagaatt
     8701 agataccgca actaaagaat tgcaggcaga aatgacttct tatattgtgt gcaaacattt
     8761 tggaatggac acgtcggaga aagctattcc ttatattgca gcatggacga agaatggtca
     8821 gacgttggat gataaggaag cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt
     8881 cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc
     8941 agagatccaa ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagagaa
     9001 taagaatatt gtgcccgttg gttcactttg gatcgacaag aaagacggga aagtaatgga
     9061 agaagatacg ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca
     9121 taatcaagtg attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc
     9181 agaagaattt gcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc
     9241 caaacgaaaa aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa
     9301 caagtaaaaa agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat
     9361 gaccgaaaaa aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc
     9421 tcgaaacaat ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga
     9481 ttcatttgtt tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg
     9541 cgacattatt gaattagcta aacttttttt catagacaaa gagatccaag actctaagca
     9601 acaatttaaa gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga
     9661 aaatcttcat tttgaaacag aaaagtacaa agatcacccc gttgattatc aaccactcac
     9721 agaaaaagga aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta
     9781 tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa tatgaacggc aaaatttctt
     9841 agttcgagac gatcgtttag atcatgcggt cgccttttta tggaaagatc cacagacaaa
     9901 agagacagta ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag
     9961 aggcacatat aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat
    10021 cggtgaccct aagcagttaa aattttttga gagtagtatt gatttactta gctatgctgc
    10081 actgaatcgt gatcagttaa acgatacgtg gttagtctct atggaagggt taaaacacca
    10141 tgtgatcagt cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc
    10201 tcaatccatc gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga
    10261 acaattaatg ggcgcagtcg atccatttac gaatcagaaa gtacgttgtg aacgaggaat
    10321 cgctaatgat tggcaagtgc caaaagaata caaaatcatt tatgaagaag tcgcaaaaga
    10381 agaaaaggtc acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt
    10441 aacgaaccaa ctggtttccg ctcacaaagt aaatgcgttt tttggccagc agctttctgt
    10501 taacgattca atagaagcca ttaatttgaa agatatttgt agagaagtcg caaaagaatt
    10561 aaaagtttgt gaacgcgtgg atggtacgta cgattttgat cgtttttatc aagaaaaagg
    10621 ggatattaat gcgcagattc ttttctctta taaagcagaa caatactaca aaggctataa
    10681 aaatcatgaa catgaattta tacctgaagt aaaaaaggac tggaacgatc aattgaagca
    10741 tgagatccag caacaagaaa tcagaaagca aaaacgtgcc atgttgtttc aacaaggcag
    10801 acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt tattttagca gaaaagccgt
    10861 cacaagcgaa agcttatgcc gatagcttct ctaaagccac tcgaaaagac gggtattttg
    10921 aaatccaaga tcgattgttt cctggtgaaa ctgtgatcac gtatggtttt ggtcatttag
    10981 tcgaattaga ttcgcccgat atgtacgatg aaaattggaa acaatggtca ttagaacatt
    11041 tgcctatttt ccctacacat tatcactatc atgttcctaa agataagaaa aagcaattca
    11101 aggtggtgaa acagcaactt caatcggccg acaccattat tattgcgaca gatagcgatc
    11161 gtgaagggga actgatcgct tggacgatca ttcaacaagt aggcgccgat caaggaaaaa
    11221 cctttaaaag actttggatc aactccttag aaaaagaagc catttatcag ggattccagc
    11281 agttacgaga cgcaaaagag acctatccaa aatttgaaga agcacaagca cgtcagatcg
    11341 ccgattggtt aattgggatg aacggtagcc cactgtatag tttactcttg cagcaaaaag
    11401 gcataccagg aagcttttcc ttaggcagag tccaaacccc aacgctttac atgatttatc
    11461 aattgcagga aaagattaga aatttcaaga aagaacctta ttttgaagga aaatccgaag
    11521 taatcgctca aaatggcgca tttgatgcga agcttgatcc gaatgaaacc caagcgacgc
    11581 aagaagcatt tgaagattat ttaaaagaaa agggggttca attgggaaaa cagccaggca
    11641 tgattcgcca agttgaaacc gagaaaaaaa gcgcggcaag tccacgtctt ttttctctat
    11701 ccagccttca gtctaaaatg aatcagctca tgaaagctag tgccaaagac actttggaag
    11761 ccatgcaagg cttgtatgaa gggaaatatc taagctatcc acgaacagac accccttata
    11821 ttacggaagg ggagtatgcg tatttgttgg atcatttaga tgaatacaag cattttttaa
    11881 aggccgaatc aatcccaacc cctattcata cgccgaatag tcggtatgtg aataacaaga
    11941 aagtccagga acattacgcg atcattccta caaaaacggt tatgacagca gcggcttttg
    12001 aacaactaag tccactgcag caggccattt atgaacaagt gttaaaaaca acggtggcca
    12061 tgtttgcgga aaaatatacc tatgaagaaa caacgatctt gacgcaagtc cagcagcttc
    12121 agttaaaggc aatcggaaaa gtaccgatag atctaggttg gaagaagtta tttggcaaag
    12181 agaacgacgg aaaagagaag gaagaagagc cgctacttcc taaagtgaca aaaggagaaa
    12241 tggtgacggt agatctacaa gtccttgaaa aagaaaccaa gccaccacag ccttataccg
    12301 aaggtacgtt gatcacggcg atgaaaacag ctgggaaaac cgttgatagt gaagaagcgc
    12361 aatccatatt gaaagaagtg gaaggaatcg gtacagaagc gacacgggcg aatattattg
    12421 aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa taagctcgtg gtgacgaata
    12481 aagggatttt gctttgccaa gccgtggaaa aagagccgct gcttacgagt gccgaaatga
    12541 ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt
    12601 ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtacca gggcaaatcc
    12661 aatccactga ttttggatcg acgttacaag aagtgaaagc agccagtgaa aagcaagaag
    12721 ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca agaagtctta ctctatcaaa
    12781 aggtggcagc atgtacgtct gaagcctgtg atttcaaact atggacaacg attgccaaaa
    12841 agaaacttac agcgacacag ctgaaagaga tcattcaaaa tagccgaaca agccaaccag
    12901 tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac gattgtttta aaagaagact
    12961 tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa cttcaagaaa agaacacgac
    13021 gcactacaaa ataatggagg gttaatctta tgcgtaatac aacacacaaa caacaagaaa
    13081 tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc agcaaacgaa
    13141 gactgcagca attgaagaac cagcagcgga ttcaaaaaga aatcgaatta gatcgataaa
    13201 ttcctttctt gttaatataa aactaacatg ttatagttgt gttagtttta tattaacttt
    13261 aggggaagag taatgaaaga caaaatccaa tgtatttttg ataaacataa tggcaaccta
    13321 tccttgaagg atgctagaag tgaaggatta tcgcctatga cgttagatcg attagccgaa
    13381 aaggataaaa tagaaaaggt agcaccaggt ttctatacct taaaggatgc gttcgtggat
    13441 gaattgtatc tcgctcagag tatctattcc agagggattt tttctcatga gactgcgctc
    13501 gatctttttc aagtaggaac atatatccct aaaaagatca atatgatgtt tcctaaagga
    13561 tacaatgtat caaaggagaa attagatcag tatgccattc aaccgcatta cactaataaa
    13621 gaaaattttg acctgggagt cacagaaaca gaaagctttt acggcaataa aattttagtc
    13681 tatgatttag agcgaacgtt atgtgacatg tggaatccaa gatacaaagc aagcgtagag
    13741 gtaaaacaag aagcattaaa agaatatatg acaagtacca gtagaaatac aaataagcta
    13801 agaagataca tggatatttt aaaatcacca aaagaaatga ctttgtatat gttgccactt
    13861 tattgagagg agtaaagatg aatgacaccg acacaattta aagagaaaac aagaaatttc
    13921 gcagcagaga aacatttaaa cccacaaatt gtccaaagat attatatgat ggaaaaatta
    13981 ttagaatgta ttgttgaatc gccttatcat gatgattttg tattaaaagg gggatttctg
    14041 attggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac gacttatcga
    14101 aattcgaaac taacaaaaga aaagttagaa actgtttttt ctgaattaac agctaatcct
    14161 acaaaagatg gaattcgttt tgaattaaag ggattaacag agacgaggga agccgattat
    14221 tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa atgcacgtgt accttttaaa
    14281 ttagatatta cttcaggtga ttctattatt ccagcagcat tgatgtatga acacaaatta
    14341 atgtttgaag ataaaacgat tcatattcca gcttatccta cggaacaaat tctagctgaa
    14401 aaattacatg ccacgtttag ttttggaaaa gataattcgc gcatgaaaga tttttatgac
    14461 ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa atttgtataa gagtgttcaa
    14521 ggaacattca aggaaagaaa taatcaatcg gtgtttaagc atttgtatga tcaggaaatg
    14581 cctcaattgg tgaaagatga gggcttgaga gaaaagtgga aaagatacca acaagaaaac
    14641 tattttgcca aagacatttc tttcgaatct acactaactt ccgttgattc cctgatgcaa
    14701 tcaattgtag agcaagagaa ggcagaacga aaacaaaatt ttaagagatc acaacaaatg
    14761 aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtaggg cgattaacta
    14821 aagatccaga tttacgttat accgcaagtg gcacagcagt ggcgactttt tccctagccg
    14881 ttaatcgtaa tttcacgaat caaaacggca atcgagaagc tgattttatc aattgcgtga
    14941 tttggcgcaa accagcagaa acgatggcga cattagctaa aaaaggcagc ttgatcggcg
    15001 ttgtcggacg aattcaaaca cggacttatg acaaccagca aggacaacgt gtgtatgtga
    15061 cagaagttgt ggccgataac ttccaactat tagaaagtaa agcagcgaca gaaagccgta
    15121 tgcacgctga tcaatcgtcc agcagtccaa gccctaccac ctttgaacaa cgggatacag
    15181 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
    15241 atattagcga tgatgatcta ccgttctaag tagcattttc tattggttgc atttgctacg
    15301 gtttatgtta taatttagac aacaaaaaga attttcaatc tataaccaaa aaccactagc
    15361 tggcgggcta gtggctttct tttacgtttc tattataaga attaacgggt aggcttactg
    15421 tctttcttgg caaatttaca atcaagccat ttgcagatat agtgcgcggc tacttctgcc
    15481 atgattgcaa aaacaaaatc aaaaagaatt ttcaaaccta taacctcctc cccttgccag
    15541 attaggccgg ggtagcagac agcaagacta ttatagcata attgaaatat ccaaatgatt
    15601 ggatttttgc tataatttta gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga
    15661 taaaatccga gtaataaaaa cgcaaccgtt attggttcgc tttactttac aaacgattca
    15721 tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgc taattatgga
    15781 tgatggaaaa tacaatattg ccgtcacagg tcacttcaat aaacgaaatc agctagttat
    15841 tgctagcatg tatgttagaa atcccgatca ttttacgcga tcgatgggca tataaataaa
    15901 agaagagttg tagtcctaaa tgaggattgc agctcttttt gttttacata gaaacaggaa
    15961 aagacctttt taggaagaca caagtgcctt ctcaattgaa aaacctttct ctcactccaa
    16021 ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa gatatggtgt
    16081 cttgcaaagc aagacaaaat agacaaaact tcctctcacc aaaaggttcg ttcctgttcg
    16141 ttatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc aaaaagcgac
    16201 aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc gttgatttat
    16261 tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct ttcctaagag
    16321 gaggcagaat caaacatgcg aaagaaattt gattattggg gtgttccctt cagcgaatta
    16381 ttcccaaact atcatgcacc acatactgtt gaatgcgatt gtggagaaag agcgaaatgt
    16441 atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac cttgtcttat
    16501 ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca caacacaaat
    16561 tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt tctgaaaaag
    16621 tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta gtagaggatg
    16681 caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg cattatcaag
    16741 aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac taaaaaggag
    16801 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
    16861 acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca aaaaacatta
    16921 caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct
    16981 cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa aatggttttt
    17041 ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac cttccgaata
    17101 tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta aagcaatggc
    17161 tagcgactgg ctagatactt ttgaaagtat cgaaaaaaat ggtgcatttt atacgattga
    17221 atcggaggag aaagataatg aataaggtgg tttatcagct aacaacagaa gatgtttcgc
    17281 aatttttaac aagcaaagaa cacttgaatg aagtaatcag acggattcaa aaagacttta
    17341 gttgtatgga agaaatgaag tactttattc aaagagtgga agaacagttg tatatggaaa
    17401 gcgaaacaag tcaaacgaag gtcacagtca gccataatca tttagacgtg tataaacgtc
    17461 gtcctgaaac ggtaaaaaaa ggaacagagc ttctacgact agaaacagaa aaatacatca
    17521 tgaatgtgta cctagaaagg agtgagctgg tcgaaaactt tgtcagctac acgatttgta
    17581 ggaaacacta ttactcagaa tcagaatatt attcaatttt cgagagcatg gaattaatgg
    17641 aagaagaaat ggaagagcta aacagcaaaa atgcgacggt agacgattat taccttgtat
    17701 ttactaggtt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg gattatgaac
    17761 tcgctgtacc cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg
    17821 actggtacac gactttaact gattttttag acacctacac ttacgacgat acagaagaaa
    17881 tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaggta
    17941 aataaccatt tataccgaaa agtgaggatg tgtgaaataa gaacaaaaaa ccttgaaatt
    18001 aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct taggtggtgc
    18061 gaaataagcg ggcaaagccc gataacctaa gagaaagaag gagatccata tgaataaaat
    18121 cattcctcta ttgttcgtac tgtttgttgg cgagatactt atgcgtcatg ctgcagctgt
    18181 acatttaaac ttcgtagtat tcttaggaat actggtattt tgtttgatct tttggcgatt
    18241 tgtattagcc cttgtgagtc tactattcaa gatgattgca gccatatttt tcatagggtt
    18301 atttcgatga aaggagagag tactaatgcc aattgcaata ggaaataaac ggttacccgt
    18361 gacattagat gaaaagagac aaaaagaatt gcagcaacta aagcagaagt acggaaaaag
    18421 tgaatctaag attatgtgta ttgcgttaga tctattgatc gcgcaagaaa aagcaggatt
    18481 tgatatacca gcactaaaaa agtgacgtca cttttcattt gaagaaacaa aagtgatagc
    18541 acttaattgt tcctactatt tagaatattt tctatgtcct ttttattcca ttttgtatca
    18601 ataactatga aatctttaaa gtatccagaa gagattattt ttagatcaat ttttttctta
    18661 cctgattcta aagattttgt tttcttaaac atattatgat ttcctttcga ctattttcat
    18721 ataaatgtta acatagttta tggattcatg gaatgttaat ctaatttaat agaaaaggat
    18781 aattaagtat atacgttttt tcttatgtat gttatactat gtatatacag aaagaggtgt
    18841 ctactatgga aatgttacct ataaaaaaat ggggaaatag taatggcctt agattgccaa
    18901 agtatattat ggaatatctt ggaattcata cagaagacaa agtgaagata atacaagaag
    18961 aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa tatagaaaat gaattaacaa
    19021 ttgaacagtt atttgaaaat tacactgatg aaagaaatca tgtgacgatt caaaatttag
    19081 gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga caaggcgata ttctattatt
    19141 gaatacagca cctcgatctg gacacgaaca gacaggtaaa agaccttata ttgttttaag
    19201 tcatgatatt attgcggatt atagtaacgt tgttacagtt gctccaattt ctagtacaac
    19261 tagaaactat ccgctatatg taaatataaa ctcagaatat gaaatgaaga caacaggaaa
    19321 agtattatta gatcaactaa cgacaattga ttatgaagcc agagagtgta tttttttgga
    19381 aaaagcgcat gatagtttag ttgaggagtt attgatgaaa gttagaacag tttttcaaaa
    19441 agtaagcaaa taaaatagaa acagcagcat gatcaactat tcgtgattga ccatgctgtc
    19501 tttttttatt ctatatacgg tttgtctcgt aacattgtag tcttttgcaa tcttagcgat
    19561 cgcaacacct ttttttagat cctcaacgat gtttttataa acaagacgac gttggggatc
    19621 ttttgcattg gcactgtata gcttaggacg tcctttataa acaccgttcg cttttgcaat
    19681 cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt tcttgttcag cgatcatggc
    19741 caaaatttga atgatcaaat ctttaatgaa tttatccagg agaggattgc caattgcttc
    19801 ggccatgatc ggtagacttg tgatgatcag ctgcacattt ttcttcttca agtaattgac
    19861 tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca caataaaaat
    19921 atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca agtcgatgag taatcgttgc
    19981 gccagattgt ttctcaatga aaattctctc tgcaccgaat tttttcattt cttcaatctg
    20041 ccgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca aaatttttac
    20101 ctactttctg caactattta aaaaacaccc taatcataca cgagaaaacc agatttttca
    20161 atgtcgcggt cgtgtctagt tagggtatac cttatataga caaaaactaa tgatggagga
    20221 aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca
    20281 agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta gtatataact
    20341 tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata
    20401 gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac
    20461 tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa
    20521 taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga
    20581 cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa
    20641 aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc
    20701 tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt
    20761 acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag
    20821 aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga
    20881 tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt
    20941 acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag
    21001 aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt
    21061 ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac
    21121 tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac
    21181 aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg
    21241 agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt
    21301 acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg
    21361 ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac
    21421 acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt
    21481 caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga
    21541 caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga
    21601 gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg
    21661 gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt
    21721 ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat
    21781 ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt
    21841 gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt
    21901 ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta ttcatttgtt
    21961 aatatta
//
LOCUS       DACEDD010000047        21846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEDD010000047 DACEDD010000000
VERSION     DACEDD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.047"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO45_002036"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO45_002036"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0333506.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO45_002037"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO45_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0333507.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO45_002038"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO45_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0333508.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO45_002039"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO45_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0333509.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO45_002040"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO45_002040"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0333510.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO45_002041"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO45_002041"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0333511.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWO45_002042"
     CDS             complement(8053..8523)
                     /locus_tag="IWO45_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0333512.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWO45_002043"
     CDS             complement(8569..8994)
                     /locus_tag="IWO45_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0333513.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWO45_002044"
     CDS             complement(9061..9330)
                     /locus_tag="IWO45_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0333514.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWO45_002045"
     CDS             complement(9341..10936)
                     /locus_tag="IWO45_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0333515.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO45_002046"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO45_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0333516.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWO45_002047"
     CDS             complement(14489..15049)
                     /locus_tag="IWO45_002047"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0333517.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWO45_002048"
     CDS             15402..16376
                     /locus_tag="IWO45_002048"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0333518.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWO45_002049"
     CDS             16546..17946
                     /locus_tag="IWO45_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0333519.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWO45_002050"
     CDS             complement(18024..18746)
                     /locus_tag="IWO45_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0333520.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWO45_002051"
     CDS             complement(18825..19331)
                     /locus_tag="IWO45_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0333521.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWO45_002052"
     CDS             19454..21253
                     /locus_tag="IWO45_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0333522.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21846)
                     /locus_tag="IWO45_002053"
     CDS             complement(21321..>21846)
                     /locus_tag="IWO45_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0333523.1"
                     /translation="PESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQ
                     PKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRD
                     IGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKV
                     GSVQWFAPDFAVSM"
BASE COUNT         6052 a         4790 c         3968 g         7036 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggc
//
LOCUS       DACEDD010000048        20670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEDD010000048 DACEDD010000000
VERSION     DACEDD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.048"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(189..1172)
                     /locus_tag="IWO45_002054"
     CDS             complement(189..1172)
                     /locus_tag="IWO45_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0333524.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1177..1824)
                     /locus_tag="IWO45_002055"
     CDS             complement(1177..1824)
                     /locus_tag="IWO45_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0333525.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1909..2541)
                     /locus_tag="IWO45_002056"
     CDS             complement(1909..2541)
                     /locus_tag="IWO45_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0333526.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2557..3108)
                     /gene="coaC"
                     /locus_tag="IWO45_002057"
     CDS             complement(2557..3108)
                     /gene="coaC"
                     /locus_tag="IWO45_002057"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0333527.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3105..3842)
                     /locus_tag="IWO45_002058"
     CDS             complement(3105..3842)
                     /locus_tag="IWO45_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0333528.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3839..4828)
                     /locus_tag="IWO45_002059"
     CDS             complement(3839..4828)
                     /locus_tag="IWO45_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0333529.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5086..5628
                     /locus_tag="IWO45_002060"
     CDS             5086..5628
                     /locus_tag="IWO45_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0333530.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5645..6730
                     /locus_tag="IWO45_002061"
     CDS             5645..6730
                     /locus_tag="IWO45_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333531.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6742..7548
                     /locus_tag="IWO45_002062"
     CDS             6742..7548
                     /locus_tag="IWO45_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0333532.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7549..8376
                     /locus_tag="IWO45_002063"
     CDS             7549..8376
                     /locus_tag="IWO45_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0333533.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8373..9446
                     /locus_tag="IWO45_002064"
     CDS             8373..9446
                     /locus_tag="IWO45_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0333534.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9874..12570
                     /locus_tag="IWO45_002065"
     CDS             9874..12570
                     /locus_tag="IWO45_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0333535.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12647..13135
                     /locus_tag="IWO45_002066"
     CDS             12647..13135
                     /locus_tag="IWO45_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0333536.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            13147..13641
                     /locus_tag="IWO45_002067"
     CDS             13147..13641
                     /locus_tag="IWO45_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0333537.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13662..14471
                     /locus_tag="IWO45_002068"
     CDS             13662..14471
                     /locus_tag="IWO45_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0333538.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14468..15289
                     /locus_tag="IWO45_002069"
     CDS             14468..15289
                     /locus_tag="IWO45_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0333539.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15340..17439
                     /locus_tag="IWO45_002070"
     CDS             15340..17439
                     /locus_tag="IWO45_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0333540.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17436..18554
                     /locus_tag="IWO45_002071"
     CDS             17436..18554
                     /locus_tag="IWO45_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0333541.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18593..19471
                     /locus_tag="IWO45_002072"
     CDS             18593..19471
                     /locus_tag="IWO45_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0333542.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19617..19814
                     /locus_tag="IWO45_002073"
     CDS             19617..19814
                     /locus_tag="IWO45_002073"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333543.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         6335 a         3560 c         4393 g         6382 t
ORIGIN      
        1 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
       61 aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
      121 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttgatca
      181 gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt tgcttccata
      241 tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc
      301 ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag ctctgcttta
      361 tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt cccaatcaat
      421 acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa gacccaacgt
      481 ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat gaaatccaaa
      541 ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt
      601 ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt ttcttctgat
      661 cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat cgctgctgtt
      721 cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa ttttccaagc
      781 cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc agaaacatag
      841 tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata ttcggcataa
      901 ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga gatatcactt
      961 ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg aggtttcaaa
     1021 gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg gactttgatc
     1081 agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa tacatctaca
     1141 gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg cccattcttg
     1201 gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac gagaaatatc
     1261 cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga tcgtttttga
     1321 tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt
     1381 gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat ctgcataatg
     1441 tttagtaata taataatctg tcatggactc aggccatttt gctggttcat ctgcataaac
     1501 agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca ctgttttgat
     1561 tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag caaaaatcaa
     1621 tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta aatcaaaaga
     1681 gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc tcacccctgc
     1741 aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt
     1801 tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc tttccattca
     1861 atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc agttagtaag
     1921 agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg caagacctgc
     1981 gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg aataaacagc
     2041 atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt agatcatatt
     2101 catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata aagaaacgga
     2161 atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag gaaccacccc
     2221 aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat gttctgttcc
     2281 gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa ctgtgctacg
     2341 gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac
     2401 gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac cgatagggat
     2461 aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaaacatcg cagttaatgt
     2521 aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt cattaagagt
     2581 cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc caaaatctcc
     2641 acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc tcaatgtttc
     2701 cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag gggcaatcaa
     2761 ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc
     2821 gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg
     2881 ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga cgtctgtatg
     2941 gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg tgctgctttt
     3001 agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa gatcagcgct
     3061 tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt tgattcctcc
     3121 agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc ttcttccact
     3181 gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc cgtcaaatca
     3241 tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac tgccagaaga
     3301 gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt tggctgccat
     3361 tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg
     3421 ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa ccaattgtcc
     3481 agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc tgaaaaacta
     3541 aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta gtgttctttt
     3601 ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt ataaatcgtt
     3661 atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc gattgtggta
     3721 tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt cgaatgattt
     3781 gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat tagaatcttc
     3841 atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca agtagtttag
     3901 atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca tctgcaatct
     3961 gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca ttgacagaat
     4021 caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga
     4081 ttcccccagc attgccacat aaagctactg caccgttata agagatcaat ggaagaagac
     4141 tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt tttcctaaag
     4201 cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca cctagacttt
     4261 ttagccatgt gacagactct tcttttctgg aaaaggcaat gattgactgg aatcctagtt
     4321 tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta acgacaactt
     4381 ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata gcagtaaacc
     4441 ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga ggaactaacc
     4501 agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg ccaagcccaa
     4561 aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt tggacgacag
     4621 ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga ccagactcgc
     4681 tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc aagacttcat
     4741 tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca ggttccttta
     4801 ttacctgaaa agatcgaaaa gcgttcattt gtgagacact ccttttaaaa ttttctttta
     4861 tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac aaaaattagg
     4921 tgaaaaataa agaatcaaac aactagaatt tattccaaaa gataaagaaa tattcagcaa
     4981 aaaaaatttt acaaatggtt gcctttttca ataaatttcg tatactaata cgagtggtag
     5041 taaacaggaa gtttactatt agaaaacttc gattcggagg aaattatgga aattggtgag
     5101 aagttaagaa atttgcgcat ccaaaagaat ttgacacaag aagaactagg cgaacgcaca
     5161 gatttgtcaa aagggtatat ttcccagttg gaacgagatt taagttcacc atcaatggaa
     5221 acattctttt ctattcttga agtcttaggt gtgactccag aagaattttt tcatcaagaa
     5281 gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata cggtttattt cgatgaagaa
     5341 aacgggtatg aattggaatg gctcgtggct gattcgaatg aaaaagaaat ggagcctgtc
     5401 ttgctgactt ttgaaggtgc aggagaatat aagacgtttg aaccttcatt atctgaaacg
     5461 tttatttttg tattagaagg ggaattagag ttaagcttag gagagacggt ctatgtggca
     5521 aaagcaggac agtcgatcta ttatcaagcg acaaaacaac atcaattaag gaatcatctc
     5581 aaaaaacgaa caaaagtatt gattgttgca acgcaatcat atttgtaata tagggggtaa
     5641 aaacgtgggg aaaccaatca tttcatttga tcaagtagtg aaacgctatg atgatgaaac
     5701 agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa ttttatacac tgcttggtcc
     5761 ttccggctgt ggtaaaacaa cgatcctacg tattatagct ggattcaccg aagctacaga
     5821 aggggacatc tattttgaag ggaaacggtt gaatgatttg ccagctaata agcgacaagt
     5881 aaatacagtc ttccaagatt atgcactttt tcctcatatg aatgtttttg ataacgttgc
     5941 ttttggattg aagatcaaga aaatgtccaa atcagatatc gaaaagaagg taaaagatgc
     6001 tttgcgtatg gtccagcttc ctggatacga aaaacgggag attagtgaaa tgtctggtgg
     6061 tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat gaaccgaaag tattgctatt
     6121 agatgaaccg ttatcggcgc ttgatttgaa actacgtaca gatatgcagt atgaactgcg
     6181 ggatctgcaa caacgattag gaatcacgtt tatttttgtt actcatgacc aagaagaagc
     6241 gttggcaatg agtgatgaga tttttgtcat gaataaaggg aaaatcgtcc aaagtggtac
     6301 acccgtagat atttatgatg agccaatcaa tcatttcgtt gctgattttg ttggggagag
     6361 caatatcgtg gacggtgtaa tgatcgaaga taatctagtt gagtttgttg gtaagcaatt
     6421 tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt gaagttgttt tacgcccaga
     6481 ggatttgacg atcacgacac cagataaagg aaaactagtc gtaacggtcg atactcagct
     6541 gtttcgtggt gtccattatg aaatcatctg ttatgatgaa cagcaaaatg aatggatggt
     6601 ccattcaacg aaaaaagcta aagaaggaag caaagtagga ttggcctttg agccagaaga
     6661 tatccatgtc atgcgtttca acgagtctga agaagaattc gatgcacgtc ttgacagcta
     6721 cgaagaatag gggggacaag aatgaaaaca atgcgcagga tctattccgt cccttatatt
     6781 ttgtggctcg gtttattcgt gatcgcgcct gtattaatga tcatttatca atcttttttt
     6841 gatatgaatg ggcaattcac gctgaataat tatctatctt attttacgtc ggggacatcg
     6901 ctttctatga cgatcaattc tgtatggtat gcatttttga tcacgttttt cacgctgatc
     6961 atcagttacc cgactgctta ttttttaaca aaactgaagc acaagcaatt atggctgatg
     7021 ctgattattt tgcctacatg ggtgaatctg ctgcttaaag cttacgcatt tattgggatt
     7081 ttcagtgtgc acggcagcgt gaatcatttc tttgatttta tcgggatcgg gccgaaacag
     7141 atattattca cagacttcag ttttctgttc gtggcaacgt atatcgagat tccatttatg
     7201 attatgccaa ttttcaatgc attggaagag atgaatcctt ctttgatcag tgctagtaga
     7261 gatttaggcg cgaatagctt tgagaccttc cgacgagtga tttttccgtt atcattaaat
     7321 ggtgtgaaaa gtggtgtcca agcagtattt attccttctc tttctttatt catgctgact
     7381 cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg ctatcgaaca gcatttcttg
     7441 gttacacaaa actgggggat gggttctaca atcggtgtga ttttaatcat tgctatgttc
     7501 atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc gctcataaat gaaaaaattt
     7561 aaatggtcgt atctttattt agggatcgtc tttttattct tatatgcacc gatcttctat
     7621 ctgatttttt attccttcaa tgaaggagga acgatgaaca gctttacagg atttacttgg
     7681 gaaaattatg aagctgtttt tgaagataca cgtttgatca ttatcgtatt gaatacgttc
     7741 atgctggctt tcttatctgc attgatcgcc acgatcatcg ggacatttgg ggctatggga
     7801 atctattaca cgaaaaaacg acgtgcacgg acagcgttgt tgagcatgaa taacatctta
     7861 ttagtctctc ctgatgttat tatcggtgct agtttcttga ttttctttac actactagga
     7921 ttcggattag gttttcagag tgtattgctt tcccatattg ctttcagcat tccaatcgtc
     7981 gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt caatggtcga tgcggctaga
     8041 gaccttggtg ctaataattt ccaagtagtc aaaaatatca ttttgcctta tctttctcca
     8101 ggaatcatag ctggatattt tatggcattt acctattctc ttgatgattt tgcggttaca
     8161 tttttcgtca caggaaatgg ctttacgacg ctctcagtag agatctattc tcgtgcaaga
     8221 caaggaatca gtttagaaat caatgcgcta agtgcgttag ttttcttgtt ctccatgata
     8281 ttggtcattg gatactactt tattagtaaa gaaaaccgtc caaaacggct gaagaaaaac
     8341 agaagagtac gcgagggggt ggcacggctg cgatgaaaaa attacaatct ctgtttttag
     8401 gtattctcat cattattgcg gtgctgctgc ttggtgttcg tcaacttgaa aaaacaagtg
     8461 gcatgagtgg agcaaaggta cttaccatct ataactgggg agattatatc gatcctagtt
     8521 tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa ttatgaaaca tttgattcta
     8581 atgaagcgat gttcacaaaa atccagcaag gtgggacaaa ctacgacatc gctataccaa
     8641 gtgaatatat gatccaaaaa atgatcaaag aaaaactagt acttccatta gatcattcga
     8701 agatcaaagg attaaataat attgatccac gatttttaga cttggatttt gatccagata
     8761 atcagtattc gattccatat ttttggggaa ctttagggat cgtatataac gataaattct
     8821 ttgatgcaaa tcaaattaag cattgggatg atttgtggaa gccggaatta aaagacagtt
     8881 tgatgctgat tgacggtgca cgagaagtta tggggctttc tttgaatagt ttaggctatt
     8941 ctttgaatag taagaatatg gcacagctga cagaagcagc aaataaattg aatcatttaa
     9001 caccaaatgt caaagcaatc gtggcagatg agatcaaaat gtatatgatc aatgaagaag
     9061 cctccgtggc agtgacattt tctggcgaag ctgccgatat gatgtgggaa aatgaacatt
     9121 tacattatgt gatcccacca gaaggatcca atctttggtt tgataatatc gttatgccaa
     9181 aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa ctttatgcta gaaccggaaa
     9241 atgcggcaca aaatgcagag tatattggtt attctacacc aaatgaagaa gcaaaagcgt
     9301 tattgccaaa agacatttcg tcggacgaac aattctatcc atctgatgac acgatctccc
     9361 atcttgaagt ctacaaagat ctaggatcga aatacctagg catctataat gacctattct
     9421 tagaattcaa gatgtatagg agataatggc aaaactcagt aagaatgggg acttatcaac
     9481 gttctaaaga gctaaaaatc gtcctttttc tatctgagtt ttccaatctt ctaccaaaag
     9541 cggatgaaac gccgcttttt ttgtgttatt ttattttttc taccacgttt ttctaccaga
     9601 gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg ggctagtaaa tctaagattg
     9661 aaaaagttac agaaggataa agtaattgga aactatgcaa atatacttaa cccttctgta
     9721 atctattaat aaaactaata tatgaatcca gttcaatatt tgtagctatg aaaaaacata
     9781 aaaatcatat tagataaaaa ctaaattggt agataaatta ttttgtcaga tatctgaggc
     9841 aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag aatagacata
     9901 ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa aatgaaaaag
     9961 agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat gtcttctgtc
    10021 actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag ccattatttg
    10081 acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt tttgtggaag
    10141 cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc ctttcattct
    10201 gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc cgctgtcaaa
    10261 tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg aaaaagtttt
    10321 ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa agatgcacca
    10381 tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc ggcaacactg
    10441 tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg tttattggca
    10501 acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa agaaaatcaa
    10561 gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga aaatgtaaat
    10621 tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga aaattattta
    10681 ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt tgaagatcgc
    10741 cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc ggatgtttta
    10801 caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca tccctttagt
    10861 gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt gtatagtcga
    10921 acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt aaatatgctg
    10981 gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa tcaaaagata
    11041 aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat tactcagctg
    11101 ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt acagcatttg
    11161 gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat gattcagatg
    11221 tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac gagtggacct
    11281 gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac agacttaacc
    11341 gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg taatcagttc
    11401 ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact gcttattgtc
    11461 gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt gcttgctcac
    11521 ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg caattatgtt
    11581 tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga atcgacaagg
    11641 aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga tatgggctct
    11701 ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact tcttctgaat
    11761 aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa tgcgccattt
    11821 aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata ctttgaaggc
    11881 ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat ttcggacaaa
    11941 ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt cacgaaggac
    12001 tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga caagacaaaa
    12061 ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat taatgtttat
    12121 gacatcttag acggtgatgg agcagccaat ttggagcata ttttgagtcc tgaaatgaag
    12181 ccggcagaat ttaaccgctt gattcaggag ctagtaaaat tcttttcgtt ggaaggaatt
    12241 gcggaccgtt tgaactttct gaacccaacg atcgtgatga gtgaagtgga aacagtcatt
    12301 tctaaatatg aaaattacta tcatttcact ttaaatggcc gtgtaaaact aaatctctat
    12361 atgcatacgg cattgatgat ggaacgttta ttcttggctc ggacagaaaa atctgaagaa
    12421 ccgaattatg atttgaaacc agatgaagat gaattttatt atgtttctag aagtattttt
    12481 caatcaatgg agatgaaata taattttaaa gtcaatgctt atgagctttc gctgctctat
    12541 gaattattag gaccgtatat tcaaaaataa agtgtctaaa aaagtgtcta atctgttttt
    12601 tttaggttag acactttttt taatgtgaat taacgtgatt aaaccaatgt ttttaaaagt
    12661 tggcacagga attgcttata taaaagtgaa cagaaagaaa ggaattaacc atatgaagaa
    12721 aattctaata gcgactcacg gtcacttagc ttcaggcttt cttagcagta ttcaattact
    12781 gacggggaag gttcaagaga ttacggtgat taatgcttat gtggatgagt gtgattttga
    12841 acaggagttg aatgtatttg taaaccaggt gaacgagcag gatcagattt ttgtttttac
    12901 cgatttattt ggcggaagtg tcaatcagaa gattaccaaa acctttttgg agaaagagat
    12961 tgctgtaact attttgtctg gctttaatct gccaattctg ctcgaaatag tattggcaac
    13021 caaggaatta acaaaggagg atattcagga actggtaaat aaatgccaac aggaattaaa
    13081 gatgaccatt cttgatgagg tacatgtagt aaatgaagag gaagattttt tttaggagga
    13141 agaaaaatga ttaaagcagt gcgaattgat gaacgattaa ttcatggaca agtagcgatg
    13201 acttggacaa agagtctaaa tttaaccggg ttagtagtag caagcgatga ggcggctacc
    13261 aacgatattc aaaagatgac attgaagatg gctgcaccat cgaatgtcaa agtcattatt
    13321 aaaacagttg atgacgcgat tcatttgtta aaagatccgc gggcgaagga catgcgcttg
    13381 atggttttag tccctaatgt caaagatgcg gtaaaagttg cgaaagcttt cccaacggaa
    13441 atcgaattaa tgaacgtggg gaatgctggg aaaatgacac cgccatcgga agataagaaa
    13501 accttatcaa aggaagtgat gttaacgacg tcagaaattc aagacttaaa agaattagtt
    13561 gaactttatc cagataccgt ttttcagcct acaccggcga tggaaaaacg ttcagctatc
    13621 tctattttga gtgattttta gaaaaaggaa ggagaaaaat tatgtatgat gcatttgtag
    13681 tcgctttggc cgtttttatt ggagttgctg gacatgaaat gttcgggatg tccatgctga
    13741 gtcgtccgat tgttgttgct ccgttagcag gagcgttgat gggggatttg cagacggggt
    13801 tgattgtcgg ggcgtcacta gaagccatct ttatgggtgt cgtcaatatc ggaatctcta
    13861 gtacggcgga gccttctttg gctgctgggc tggctacagc gttcgcaatc aaaatgggag
    13921 gaaacgtgga tgcgattatt cccattgtct ttccactagc agtattagga ttacagttaa
    13981 tgaatatgat tttttctttt gtatgtggac cgatggctgc taaatttttc aaatttgcaa
    14041 aatctggtga tcaaaaagga atcatcaatc tgcacttctt tatgtggttt gtccactatg
    14101 cgctttatgc tcttattcca ttctttgccg ttttatttgg agcagatgtt gtcaaaagcg
    14161 tcttggataa tattccagag gtcatcatga acggattaac agtcgctgga aatctgctgc
    14221 cggcagttgg gatggcgatg ctgttacgga tgctttggag taaaaatatt gctgtgtggt
    14281 ttttctttgg ggccgttgtt atggcgtatc tcgagttacc attgattgca attgccgcag
    14341 ttggaacgat tattgcaatt gttatagccc aaagagatct acaactaaaa aatatatctg
    14401 ttcatgcagt tcaaagcgat gctgctgtaa atgcaacgct tgaccaagaa gaggaggatt
    14461 tctttcaatg aaattagctg atggaatttc taaagatgag aaaaaattgg ttcggaagat
    14521 gttttggcgc tcgatgacta tgtatgcttc ggtgaatccg gtgacgatgg gcggcggtgg
    14581 tttcgcctat tctatgatgc ccttcattaa tcatttctac aaaagtgaag aagatcgagc
    14641 tgcggcgctg gaacgtcata cacagtattt ttcgacgacc attccttgcg cgtcctttgt
    14701 catggggatt gcggcatcga tggagaagga aaattctgaa aaagctggtt ttgacgtgga
    14761 aagtatcaac gcgatcaaaa cgagtttgat gggaccattg gctggaattg gcgattcatt
    14821 gttctgggga gtttggcgtg tcgttaccgc gggactagcg atcaacttag ccaatgccgg
    14881 aaatattttg gcaccaatta tctttttagt tatgttcaat atacctaatt acttgatgcg
    14941 atactatggc ggcgttttag gctattcatt aggctctaaa tatattgaaa agctgtatgc
    15001 gaacgggtta attgaaattc tgacaaaagc tgctagtatc ttgggtctga tcatggtagg
    15061 tgcaatgacg atcagtatgg tcaaattcaa taccgttttg agcttttcaa tgaagggcga
    15121 agaactaatg aatgtccaag aaatcctcga tagtattttt aaaggaattg taccgttatt
    15181 ggtaacattg agctgtttca aattactaga taaaaaagtc ggcgtcatca aaattatttt
    15241 tggaatggtc gtcttgggaa ttttattgtc attacttgga atcgcatagt aggaagctgc
    15301 agctactagg ctgcagtttt tttagcaagg agggaaaaga tgactattaa tgaagtaatt
    15361 tattcaccca aacacccatt ttttaatgct gagttaaacg acggaaatgt gactacttca
    15421 aacgaataca gctactatcg gaatattcct gatgtaaagg gattgacctt cgatgtaagt
    15481 aatcaaaaga cattagcaaa ccttgatgtt tgcgggacga ttcaaacgtt gaccttttat
    15541 cgagaaaatt tactaactga ggaaaaacca ggtgtttggg tcaataaaca gctctctcaa
    15601 accaattcgt tatccttaaa ggttgaagta aacgggattg tctaccattt gtcagaatcg
    15661 gatcatcgta ttgaagtcga tgtgattcaa gactgtttgc ctagatatat tcatcattat
    15721 tcgacgttta aggtcattgt agtatccttt gctcccatac tggctaataa gcgtttaagt
    15781 atgcttgttc aacaggttta cattgtgaat gaaacacacg aaagccttca agtttcagtg
    15841 aaagatgtgc cttcgtatca agagaagtat tcggatcagc aaaatgtgtt gatttctcaa
    15901 aaaggaaata aggacacgat cgcacaacag gaagtggcaa gcttcgccat tgcttttgtg
    15961 gacccaaatg cttttgaaga agtaatgact tttcaggaat ctgatatcga aaaatggctg
    16021 aaggaaacat tgaactattt tgctcagcta tttgggcagc tgtcaatgcc aaacgataga
    16081 gttgtccatc tctatcgtcg tgcactttat caaagctttt ctagtttcgg tatgaatcga
    16141 cagggacagg tcgtcggcag caattggggc agctatcctg ctactaatcg aatttggaat
    16201 aaggatatgt attacgcatc attgccattc accatgtttg aaccggagtt atgtcaaaaa
    16261 acggttctat ggtttgatca atatggcgtt aagtttcctg gttcaaaatt tacaggaggt
    16321 atcaatcatt ctctgagtaa ttcgttatcc agtgcgttac tggctagtct ttattttgaa
    16381 catactgggg atacaaaatt ctttgagcgg tgtccgcagg ttctactgaa tgcttcaaga
    16441 attattgagg acatattagc tcaacgtcgg tcaggggaac caatgctttt tccatctgtt
    16501 tggttatcag acgcctttgc acttggaaag tatcataccg gcagcaatct ttgtctttgg
    16561 aaggcctgta ttggacttgc agaaattttt gaagccctag aacaactgag tcttagcaag
    16621 cgatacaaaa acattgcctg taaactaaag gaatcaattt gtaaacaaat gactatcgat
    16681 ggtccttttg gtgagcaatt tttggaggga atcggtgatg aggagaaaac aacctattca
    16741 gtgaagcact atcaaaaacc gattcttgag caagggttaa tctttttaag tgatgtgatt
    16801 gtggatggaa aaattaattt attgatgcat gatggagaag aatcagacac tacgttgatg
    16861 cctttttatc aatttttaga taataaagat catttatatc agaacaccat ggccttttct
    16921 gctagttctt tcaatccaac ctatagcgag gaaatcaaag gcatcacttg gggattagaa
    16981 tccggcgcca cttttcctgg ctttatcact gttttaatga gcgagctgca caaccctcaa
    17041 gctttcgtaa cccgattaga agaactaatt tgtttggcag atttagacgg ttcgtggtgg
    17101 tggtggccgt atcgcttaga ggctcaacaa ggagaagtcg tgcgagactt tggctgcggc
    17161 aaatgcggct gggcatcggg tctttttgtc agcctaatga tcagtcaata tttcggattg
    17221 aaattcaaaa aaggagtatt gcaaattgat cctgttgata atttaacgtt ttcttggaag
    17281 aatttgaagt tcggtcaagc cttcatttcg attgaatgta cgcaatccga gttgagtatt
    17341 tcaaatttgt cagaaaagcc attgaaaata agtgatacga aaaaaattct tcacgtagaa
    17401 aagggaaaaa cgattcacat tcaaaggaga aaaagatgag tatactaaaa aatattaccc
    17461 aatttaaaaa aatccagagt cgagcaatca ctcccgaaaa tccaacaggc gagaaaggta
    17521 aagctgcgat gttgccaagc gagctaggac caagccgcaa aggtcgtggc gcaattccgc
    17581 tgacttctgg tgaagaaact gtgattgcgg atattactgg aacgggaata attcgtcata
    17641 tgtggatgac gattcgtgat cgaacagaat ttggcagttt tgttttacgt gatttaatct
    17701 taagaattta ttgggatggg tcggatagcc ctgctgttga aagtccttta ggggatttct
    17761 tttgtaatgg gtttggagag cgttgcgagg tgaactcgtt gccgatagtg gttaatccta
    17821 cagggggctt taattcctat ttcgaaatgc ctttccaaac atccgctaaa attacgatcg
    17881 agaatttaca tccgaaggat atgtcctcat ttttttatac gataaattac gctttggtgg
    17941 acgaattgga agaaaatagc ctatattttc acgcaaattg gcatcgggaa cgcattacca
    18001 aattacagga ggattataag ttaattgatc gattaaaggg tcaaggctat tacgtgggta
    18061 cttaccttgc gcttacctgc ctagaacgtt attggtgggg cgaaggcgag tttaaattct
    18121 acattgatgg ggatagtgag tatccgaccg taacctcaac aggttcagaa gattattttg
    18181 gcggggcgtg ggcctttcat gagtacgatg agttcggacg tccacatgcg aaaaactatt
    18241 caacagcctt tttagggtat ccttaccaat caaatagaga tgccacaaga gatcgttttg
    18301 aaacgggaaa attgaatgcc gttcatgcct ttggtgacga cgcaatgcca cgacatggtc
    18361 tttatcgttg gcatcttcaa gaccccatct gttttaccga agatttatcg gtcactttac
    18421 aacaaatcgg caacgatgat gttcgcttat ttgagcggca agacgatgtt gctagtgtcg
    18481 cttactggta ttcaacagaa gcttcaggaa atactaaacg tcatttcact gatcgagaac
    18541 gcttgccgcg ttaatgaagt gtttttcaca tattcgaagg gaggaaccta tgatgatata
    18601 tggggcaatt gaagctggag ggactaaatt tgtctgtgca gtcggtactg aggaattaac
    18661 gattttggaa agagtcagtt ttcccacaac aactccagtt gaaacaatga agcaggtcat
    18721 tgattttttt aaacctttta aggaacaatt ggcaggcatc ggggtcggct gctttggtcc
    18781 aattgatatt caatgcaaat caacgagttt cggtcatatc acatcaacac caaaacttgc
    18841 ttggcaaaat ttcaattttg taggaacgct aaagcaatat ttcaatattc cgattgaatg
    18901 gacaacagat gtcaatgcgg cttgctatgg tgaatatgtt gctggttctg gcaaagggtt
    18961 agcaggtgtt gcatacttca ctgtgggaac aggtatcgga ggtggtgcct tgttcaatgg
    19021 gactttcgtg gaagggttca gtcatccgga aatggggcat atgttgatca agcgacatcc
    19081 aaaggacaat ttttcaggaa actgtccctt ccaccaagat tgtttagagg ggatggcagc
    19141 aggaccagcg attgaaaaac ggttgggtgt aaaaggccag aatttattgg ctgacgattc
    19201 gttttggcag attgaagcct tttaccttgc ccaatgtgca tacaatacaa cactgatgtt
    19261 ttcgccagat agaattattt ttggcggtgg tgtaatgaaa caagaacaca tgaagaaaaa
    19321 agttcaggat aaatttgttg agcttataaa tggatatgtt gaaataccac caattgatag
    19381 ctacattatt acgcccgaat taggtgataa tgccggaata atcggcggat tagcattggc
    19441 cagaaaagcg gtaagaaata agcaaccata attaatgaat gtttcattga tactcatgtc
    19501 aaaagtattt aaccgagtaa tcaaatgatc aaatgaagtt ggataaagcg aaaatgaatt
    19561 caaataaaaa ggctctaagt caaatcgtgt tgttttcatt aaatcactga aaggaaatgc
    19621 ttctgcgttt ccttttttct ttttgttgtg cgctgtatac cttgaggata ttcactggtg
    19681 gcgtacactt aaatagtaat atattgtgtt actttatgtc tgtggtaata gttatagttg
    19741 cttaccaatg tatgttcatt tttcgaaatg ttgacttcat aatccttggc ttaatgtttg
    19801 aatttttttg gtgatagatt ttttcagccg tataaaataa atcgtaattc tattatagag
    19861 agttgtgatt ataaaaagat agcaagaaaa aaaaagaaag tttctataca ttttaaatat
    19921 tttattccta tttctattgc taaaaaagta gaaattctat atttgccttt aactctcgct
    19981 tctcttgcaa atgtagttag cattttaatc gataataaac tcaggtagtt atattacctt
    20041 gaaatgattg ataattttgt cacttctatt ttttttgcaa taaatttttt attttagtga
    20101 aaaataagaa actatttctt tgaaggcgat aagttagaaa aatattattg tagtcttttc
    20161 tttattagga atcatcactg ttgtatctta ttaccttttc tgtttttctt attagagaaa
    20221 aataatttga gaggtagata gtttttattg gaggaagtaa ttatgtatat ttttgaaaca
    20281 aaaatttttc attgcaataa ttgcaacaaa gaaatttcat cgggggaaga ggtttggaca
    20341 aaaatggaaa tttccgccaa aagtaactgc tcctcaactg aaatcaatta aagaactaga
    20401 atttgagaat gcactggtaa tttgtatgac atgtactgat gaagtatttc gaaaacattt
    20461 ttaaaggttg agatgtatat agatttaacc aatcatttat ctctctcgga aaaggaatat
    20521 taactaacca gcgggttatc aaaaataggt aggaaaactg tacttaatgg cttatttcgc
    20581 gctgattatg agaatatcag tctgtttgat taatttttag ggttcaaaat acagagacaa
    20641 actccaggtc gggtttcgcc acaagcgaat
//
LOCUS       DACEDD010000049        20566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEDD010000049 DACEDD010000000
VERSION     DACEDD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.049"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1141)
                     /gene="mutY"
                     /locus_tag="IWO45_002074"
     CDS             complement(<1..1141)
                     /gene="mutY"
                     /locus_tag="IWO45_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0333544.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVD"
     gene            complement(1134..1931)
                     /gene="recX"
                     /locus_tag="IWO45_002075"
     CDS             complement(1134..1931)
                     /gene="recX"
                     /locus_tag="IWO45_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0333545.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2038..3420
                     /gene="rlmD"
                     /locus_tag="IWO45_002076"
     CDS             2038..3420
                     /gene="rlmD"
                     /locus_tag="IWO45_002076"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0333546.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(3594..4172)
                     /locus_tag="IWO45_002077"
     CDS             complement(3594..4172)
                     /locus_tag="IWO45_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0333547.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            4494..4964
                     /locus_tag="IWO45_002078"
     CDS             4494..4964
                     /locus_tag="IWO45_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0333548.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5072..6298)
                     /locus_tag="IWO45_002079"
     CDS             complement(5072..6298)
                     /locus_tag="IWO45_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0333549.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7052..7252)
                     /locus_tag="IWO45_002080"
     CDS             complement(7052..7252)
                     /locus_tag="IWO45_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0333550.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(7386..7994)
                     /locus_tag="IWO45_002081"
     CDS             complement(7386..7994)
                     /locus_tag="IWO45_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0333551.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8079..8549)
                     /locus_tag="IWO45_002082"
     CDS             complement(8079..8549)
                     /locus_tag="IWO45_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0333552.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(8597..9511)
                     /locus_tag="IWO45_002083"
     CDS             complement(8597..9511)
                     /locus_tag="IWO45_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0333553.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            9657..10460
                     /locus_tag="IWO45_002084"
     CDS             9657..10460
                     /locus_tag="IWO45_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0333554.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            10638..11585
                     /locus_tag="IWO45_002085"
     CDS             10638..11585
                     /locus_tag="IWO45_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0333555.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(11653..11946)
                     /locus_tag="IWO45_002086"
     CDS             complement(11653..11946)
                     /locus_tag="IWO45_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0333556.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(11974..12639)
                     /locus_tag="IWO45_002087"
     CDS             complement(11974..12639)
                     /locus_tag="IWO45_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0333557.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(12779..13411)
                     /locus_tag="IWO45_002088"
     CDS             complement(12779..13411)
                     /locus_tag="IWO45_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0333558.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(13418..14485)
                     /locus_tag="IWO45_002089"
     CDS             complement(13418..14485)
                     /locus_tag="IWO45_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0333559.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(14482..15213)
                     /locus_tag="IWO45_002090"
     CDS             complement(14482..15213)
                     /locus_tag="IWO45_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0333560.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(15382..15861)
                     /gene="greA"
                     /locus_tag="IWO45_002091"
     CDS             complement(15382..15861)
                     /gene="greA"
                     /locus_tag="IWO45_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0333561.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(15997..17172)
                     /gene="mltG"
                     /locus_tag="IWO45_002092"
     CDS             complement(15997..17172)
                     /gene="mltG"
                     /locus_tag="IWO45_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0333562.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            17376..18545
                     /locus_tag="IWO45_002093"
     CDS             17376..18545
                     /locus_tag="IWO45_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0333563.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(18812..20434)
                     /locus_tag="IWO45_002094"
     CDS             complement(18812..20434)
                     /locus_tag="IWO45_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333564.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6369 a         3998 c         3226 g         6973 t
ORIGIN      
        1 gatctaccag tttcatttgg accttaggaa aaacgaccga atcaaatgca tcaggcttca
       61 accacttcga agttttgttc tcggtaaatt cttgttctgc tccctcggtt gcccgtccgt
      121 aaaaaaggag aacatgccat ttcagatgac taaaaatatg agtaacttct cctaaatgtc
      181 gtgtctgcca aacaaagagt tcctgctttt caaatgggag attttgctct tcttctgcta
      241 ctaggtcatc aaacagatcg atttctggct ttgtttccca ttcttttttc aatcgctcat
      301 actcctcttg tgtcacttcc atcattggaa atgtccacat attcgccagc aatttttggc
      361 tatctctttc ttcaaaataa tacgctccag aatgattttg aagagctgca ctgatataat
      421 aaacgtcttt tggctttgct ttttttgttt ttacaggaaa actcgtttgg attcctcgtt
      481 tgttagctaa acaaaaggct tggatcggac aagtctcaca ctttggagaa gttggtgtac
      541 agatagctga acctagatcc atcattgctt ggttgaattc acctggatgc ttttcatcaa
      601 tgattttccg catcgcttca tcaaagattt tcctgctaga agcttttgcg atgtctgctt
      661 caatgcaaaa caatctgctg actactcgca tcacgttgcc gtcaactgca ggttctggaa
      721 gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg tcctattccc ttcaatgagc
      781 taatttcttc aggcgtttgc ggcatttccc catcaaattc tgacatgatc tgtttagcag
      841 cagcttggat gttccgggct cttgagtaat aaccaaggcc ctcccatgct tttaaaagct
      901 tttcttctgg agcatttgct agctcttcaa ttgtaggaaa ccattccatg aaacgataaa
      961 aataatcgat cactgtgtct actctggttt gctgaagcat gatctcagag atccagatac
     1021 gataaggatc acgattatag cgccaaggca ggtttctttt ttcttgttca taccattgaa
     1081 tgaactgatc ttgaaattct tttgtttctt tatctgtcca tttttcccat tctttattca
     1141 ctttcctctt ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa tcctttttga
     1201 tataagcttt gctttacttt tagatttctt tttttaggtt caaaccttaa atttcttcgc
     1261 catagtttat ctccttgttt gactaaataa tcatattcta tttcttgatc tgcttcttgc
     1321 ggaagatcag ctatcgcttg ttggatcact tcttgagaaa agcctttcgt catcaatgtt
     1381 tgttggattt ttctcaataa ttctttttgg ctttttccgt gattttttcc aaacgctttt
     1441 tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg tatattggct gagcgcttgt
     1501 tcaatgattt caggtttcaa ccctttttgc tgcatctgct gcgctaattt ctttggtcct
     1561 ttgtcactta agcgcatatt cgtgcgcaca taactttccc catagaccaa atcatcgaca
     1621 aggtccaatt cttttaagcg ggctacgatt ttatttcgat cttcttgaag gatttccttt
     1681 tcttttaagt atgttcggat ttctttttcc gatcgcaact gataacttaa gtaattcatt
     1741 gctaggtgaa aaccgaaatc agctccgcta ctcttcttga tctcttggat agtttcttcg
     1801 tctaattccc gtccttttaa taatcgatag cgtactaata catcttcaga tacttgtaaa
     1861 gcttctcctt cagataaatc tacgcgatag aaagggcctt tgcctttact gattctcgta
     1921 acggttaaca taaacatcct cctcgaacat atattctcta tcataccaca gaaaatcccc
     1981 gtaatcaggt attatatgtt agaataaacc acagaaaaaa gaaaaaggga gatacttatg
     2041 gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg tttaggcata
     2101 aatggtgaag gaatcggtta tttcaaacgg ttgatcatat ttgttccata tgcactacca
     2161 aaagaagaag tactagtgaa aatcacgaaa gcaactcctc gctatgcaga aggagaatta
     2221 ataaaagtca aaaaaacaag caaagatcgc gtagtggcac catgtcctgt ttactatgaa
     2281 tgcggtggct gtcaattaca acacttagct tatcaagctc aattggattt taaaaaggat
     2341 ctgcttttgc aagcattgga aaaattcaaa ccagcaggct ttcgatctta tcaacttttg
     2401 ccgacgatcg ggatggatga gccttggcat tatagaaaca aagcacaatt tcagttacga
     2461 aaaaataagc agactcaaaa aattgaagct ggtttatacg aagcaaactc tcatcagtta
     2521 gtccctataa cagactgctt ggttcaagaa cctcgaacac aaaaagtaat gaatactgtt
     2581 gttcaactat tgaataaatt tgatgcaccc atctatgatg aacggacaaa cagtggaatc
     2641 ttccgcacaa tcatggttag aatcggtgtt aaaactgggg agttacaagt tgtttttatt
     2701 actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa tgaacgactg
     2761 cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct cgtgatgggg
     2821 gatgaaacga tccacttatg gggaaaagaa gccattgaag aacaaatcaa tgaagtgact
     2881 ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga attgctttat
     2941 ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga tgcttattgc
     3001 ggagttggga cgatcggctt aagtgtagca aaacaagcca aagaagtacg gggaatggat
     3061 accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg atatcacaac
     3121 actcgatatg aagtcgggac agcagagcaa gttctgccta aatggttgaa tgaaggtttt
     3181 cagcctgatg gtatcatcgt agatccacca cgtacgggtt tggataacgc attgatccaa
     3241 tcattattga aataccctcc aaaaaaactt gtttatatct cctgtaatgt ttctacactt
     3301 gccagagatc tggttaattt aagtaaaaag ttcaagattg aatatttaca gtccgtcgac
     3361 atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact cgctgactaa
     3421 atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa taagaacagc caaacaaaaa
     3481 tagctcttca tgttttatga atactgtact tgtgaccgaa gagttaacag gaggacacta
     3541 tttatttaaa aaaagggttg tgacatagtt tttgtcacag ccctttttga ttattattgg
     3601 atattcacgt ctatcacttg ataaaatgca tttgtcgtat cgctgatatt ccatactgct
     3661 aaaatcacat tatatccttt tcgatcctgc ggaagtgtga tcgtttggtt gactgttttt
     3721 ggtggaacag ttgctttgtc gtctattttt gtgatcaatt caaaatcact aaatttcaaa
     3781 ggttgattag gattccaagt tggtttggta atgtagtaat cccaagtact tgtccgatgt
     3841 tgtgctgtta aattccaagt aatatttaat gttcctggat taaccatgtt tttatgccat
     3901 ctgttagatg tctgttcatc taaaggctca aagccgctga ctcctgcact agcaattttt
     3961 ccatcaatga atgtattttt ggtagcttcg atactttgcg gttcccattg tgctcttccg
     4021 acattttgat ttaagtttcc tgctgctgat gttcctaggt atgcacggct tcctggagaa
     4081 gtaatataac catgcgcaaa acctggaatg ctaaaaccga acaaccctaa actaactaac
     4141 ccaataaaca ataacgtact aatctttttc atgtagaaac ctccttcatt tgataaccac
     4201 attgtaaacg ctttattgta ttaacacaaa cattatgctt ttataatttt atgttgaagt
     4261 aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag tagacgagaa aagtacaaat
     4321 agaaaacatg agaatcaaat catatgatct ttttcaaaaa catcattcat ataaacaaat
     4381 gttcaacttt ttaataacaa caattatcaa tttttttctt acttcacaaa ctaaatacag
     4441 gtcttgctgt gagccttcat ggtaaactaa tattatagaa aggagttagt aaaatgaaga
     4501 tgataaaaga tattcgtttt tgggtttgtg ttataggaat tgtcatatta ggctttttat
     4561 cagggttgct tagcggaaat cccggaaaat attactattc tttgcaactt ccaccatttg
     4621 caccacctag ctggatattt gggccgatgt ggaccttact gtatatattg atgggtattt
     4681 ctctttattt gttacttaat cacaacaaca aaaaacagcg aaacaatctt gtcggtcttt
     4741 ttgtgattca gttcattttt aattttatat ggtctgctct attttttaat ttaagaaata
     4801 ttttcattgc agcaatcgat attacacttt tagttatttt cttgtcagtg cttatgtatc
     4861 agttgtggct gcatcatcgc ctggcaatgt ggctcatgat tccttattat ttatgggtac
     4921 tattcgcaac tctattgaat tattcaattt attttttaaa ctaagcgcta aaaagagacg
     4981 tcctgtaatt taacacatag aaaaagtgcc acctaagtta gattttttgt ctaatttagg
     5041 tggcactctt tattaaccaa actatttttt tctagtaccc acttgttgtt ccatactcat
     5101 tattctggtc ataaccataa ccattttgtt cctgtccagc gtaaggatca taactttgag
     5161 tatcttgact tggtgtatag tactgacttg tatcttctgt ttgctgataa taggtattcg
     5221 gatcttggta ctgatcatat gtgttttctg aataggaaga ataatcggtg tacccttcac
     5281 tattcgatcc atcaccgttt gtcgaatcgc catcttccga atcatcaaag aacatcattg
     5341 aagcattctc atttttcaaa ttaggtaatg tatcgcttgt ttttatattt aattgtttct
     5401 tcagttcatt ttggatcgaa agaagatcat tttttcctaa aatttgatag tagatatcat
     5461 tgatcatttg tccttcgcct tgcaactggt cttgtttgat tgtttcaaac gccggggtgt
     5521 agttcgtcgc tacatcaagc atgtcatccc aatctaagtc cgtcgtcaca ttgttctcaa
     5581 ctgcttttaa aatttttcta taattgctta cgccatctaa acttaaaact ttattgacga
     5641 ttttcgtcac tacttcccgc tgacgacgtt gtcgaccgat atcgccttct ggatcttcgt
     5701 gtcgcattcg agaataggct aaaccttttt ctccattcaa tgtttgttca cctactggta
     5761 catggacacc atccaaagta aagtcaattt tattattcac tttgatgcca ccgaccgcat
     5821 tgatcaaatc ttccatgcca tccaaattga tcgttacata atgatcaata ggaatatcga
     5881 gcagttgctc aattgaatcc atggccattt cgacaccacc aaatgcgtag gcatgattta
     5941 atttatctac cgttccataa cctacgatat tcgtataaat atctcggtcc aaactgatga
     6001 tcgttgattt tttttgtttc ggattaactg ttactaccat catcgtatca gaacgtcctt
     6061 gttccgttct tcctaatcca ccggtatcta aacctaatag caaaaccgaa aaaggttctt
     6121 tgtcatcaat gcttactctc tcagaacgtt tggaggatat tcgattagag tttttagaaa
     6181 ttttgtttac tgtttggctc gcttctccca taaatctgat tccgtatgct gataatccgg
     6241 caatcagcaa aacgaatacc cctaacaagg ttaatagaac tttttgccat cgcttcataa
     6301 tacacctctg tttcacattt tctaaaatat tgtctcatat aactgttcaa tcagcaaatt
     6361 cactttttaa tcataagaaa actttaaaaa acatttttgt gtaacgatta ttatttttta
     6421 aaatttctca aaattcttaa ctatcatacc ataccgtgaa aaagatcgaa agaccgaata
     6481 cacgcagttc gcaatcgttt tatctttttt tatgtatttt ttccagaaaa agcgaacaac
     6541 aaggtatttt ccaaataatc gtgcagtaaa atgatcgcac atacctttcc catcttttta
     6601 acagtctttg cctaccgttc agattatcat tcgatttcac gacaatttct tcctatgact
     6661 ggtccttttg ttaaacccat cttctattcg ttttatttcc agctcactgc atggctctta
     6721 aaatgactcg aactagtgat tgcattacca tcatctttta atggatagat atgctcctct
     6781 tcaaagtaaa acgattttct tttaaagtaa tagaattgac atggtaatga gtatgtatac
     6841 tagtggtttc acaaatggtc ttttattcaa catccaaaaa ctgaaatttc tttaaaatcc
     6901 attactaacg aagaaaatgt tccttgcttg ataattagtt aggacatgtc aagtgaacaa
     6961 atgcatttac ttagccaaaa aatctaaaaa cttcataaat agacaaaaag acacgactct
     7021 ctaagaagtc gtgtctttat tcaacaaatt attataattt agtcacttca gcagcttgag
     7081 gtccgcgtgc accttcaaca atcgtgaatt ctacttcttg accttcttct aggctcttga
     7141 agccgtctcc ttgaattgct gagaaatgaa caaatacgtc atctccgccg tctactgtaa
     7201 taaacccaaa tcctttttca ttgttgaacc atttaaccgt tccgtgttcc ataaatgtat
     7261 atcctccaaa aaagttcttg caagtaacct tgcaagaaca ttatacccaa tttagaaaac
     7321 gctagcaagc tttcgctctt ttattttcca aattattttt aagactgata tgacggagga
     7381 aatggttatt ttgctgcttc gaaacgtttg tttacttcat cccaattaat aacattccag
     7441 aatgctgcaa tataatctgg tcggacattt ttatatttca aataataagc atgttcccaa
     7501 acgtccaaac ctagaattgg tgtctttcct tccatcaatg gagaatcttg atttgcagta
     7561 gaggtaatag ctaattttcc attttccatt acaagccaag cccatccaga accaaatcgt
     7621 ccagcggctg cttttttgaa ttcttcttta aaagaagaaa aatcaccaaa agcttcatta
     7681 atcgcttctt ttatttctcc tgtaggttcg ccaccagcat ttggtgccat gatttcccag
     7741 aaaaatgaat ggttagcatg tccgccacca ttgttacgta cagctgtctt gatatctgtt
     7801 ggaatagcgt ccatatcaga taataattct tctattgttt tttcgcctaa ttctgggtat
     7861 ttctcaattg ctgaatttaa attcgtcaca taagtattgt gatgcttatc atgatgcaga
     7921 tgcatcgttt cttcgtcaat ataaggttcc aatgcgtcat atgcataagg tagatctggt
     7981 aatgtgtaag tcataaaaaa attcctcctc aatgttttct taataatgtc tacattacaa
     8041 agagtaacaa agagaaggaa attcttcaat tgttatgcct agtcgtcttt ttcactttga
     8101 cctacttctt cttcaaaaac ctcagtttct ttaaaaataa acaacttgct gaaaatatag
     8161 ttcgcaagga taacaataat ttgagtgatg attttcgcca catactcatt gctgttcata
     8221 acattgatca acaataccat tgcccccatg tccaatccgt aagaaaggat acggtagaag
     8281 aagaacttac cgcattcaat aagtgcatgt gtaaaattcg gtgattttga atggaatacc
     8341 caaagtttgt tcgttacaaa agcaaacaat acagaacaaa accacgaaat agaattggat
     8401 aaggccatac ttaaatcaaa atattggcgt gcgagagcaa aactaacaaa atttacaatc
     8461 gtagccaaac caccaaaaaa gagatagact agtatctccc aatatccttt tgcttctaaa
     8521 tatcctttta actgtcgata ctgcttcatg agccaaccta ccttattaaa aattcttatt
     8581 tatcatactc tatatttcaa taactttcat ctttatttaa aaaaattaat tttttttgat
     8641 cctttgcctt tgtctctgct tggatcgtca catgtgtaat accatatttt aattttaata
     8701 aagaaattat acgaggatat atcttttcaa tctcgcttag tagctgatct tcaaaatcaa
     8761 tatgtccgtc gaaaataatt atcttttcgc tatactgcca aatatggaca tgatgaacat
     8821 cctttacccc atcgattgct tcaatttctt gttccatttt ttcatagtcg atagcaggtg
     8881 cactttgtat caaaatagcg gcagcttttt tgatcaccat tatcgcttct atcaatatat
     8941 atatcgaaat gatcagtgta ataactggat cgatccagta gatatcgaaa aaacggatca
     9001 aaattgctcc gataatcaca cttaccgaag acagtgtatc acttaacaaa tgcaaataac
     9061 tagattttat attcaaactt tcatctgctt cttgtcgtaa taataatact gagaacaaat
     9121 tcgcgagcaa tccaataact gctactgtta gcatcaaatg actatttatc ttttctggtg
     9181 aagtgaaccg cttgaatgct tcaacggtta aaaacaagga taatatgatc aatatgaatg
     9241 agttgatgaa agcactcaat atttctaaac gctggtagcc atacgtacgt tttttatttg
     9301 cttcttttcg ggccattttt tgtgccccgt aagaaaaaat aattgccaat gtatcactaa
     9361 ggttatggaa agcatctgaa acaagagaca aactccctga cataatccct ccaataaatt
     9421 ctgataaagt gatcaccgca ttcaaaacca ctactccaaa tagttttttt gatgatctct
     9481 gtggctgtgt aggcactatt ccttcctcca ttttatttat attggatagt ctttagacaa
     9541 gaataacatt tttttgatat ttcaccaatc attcagctgt ttccttaaag cacttgtatt
     9601 tcacttgata cttcgctaga attgcaagtg atgacactac tagatgagga gtacttatga
     9661 caacaaaaca attaattatc agttccttta tacgattcaa agacctaaag gaggcgagaa
     9721 atgtcccatt ttggaaagcc attgtctatc ttttagtttt gagtataatt atggctttgc
     9781 caatctcttt tcaagttttt caagtcttaa acaacattca attggatggg caaaagattg
     9841 ctactcgcat tcctgatttt acaatccaaa acgggcagct tcaaaccgaa gaaaaagaag
     9901 gattcattta ccaaacaaac tccattatct tcacatttga tccagaggga aaacgaacag
     9961 aacaggacat ctctactgat ttgatgggga attttgttag tgtcgggatg ttaaaaaaca
    10021 aacttatcat agctcttcct aatacaggca cgacttctgc tttgttgaac aataaccaat
    10081 tagaacttcc ctacaccaat gaatctttaa aaaacttaac aggtaagcag ctccgttctt
    10141 ttttaagtga agcatctata ccactttgga taaaagtcct gacatttata ttttctattt
    10201 atcctagttt tctgaatctg ctgatcactt tgctatttac caacattgct gcttttctct
    10261 atgcacgctt tcgactaaca aaagctactt ttcttgattg tttaaaaaca ttgatctatt
    10321 caatttcatt gcctactata attgcaacaa tcttgatggt ctttcttcct tcttttgaca
    10381 ctagcgcctt cattgctttt gcaggaatct ttatttttgc tcaagctgtc aaaggatggg
    10441 caaaaatatc gatcaattaa gaaataaaga caactcgtta tttctacttc aatagaccct
    10501 tttagaggtt gtgacatttt tttgtcacag cccctttttt atttgaaaaa tatttctttc
    10561 tcaaaaaaca agcaaggcgc ttcaaagttc ttcttgtatg cgctacactt agagtaaaga
    10621 aaggcgggaa attaactatg tcagaaagaa tcatttatgc agatcgtcca tttcgtgatg
    10681 aatttatcga agaaatccat actatagcgc ctggttatat ctttaaaaca gaattacaac
    10741 cagaggatct acaaaatgta gagatcagtt tgggctggaa ctcagactac caagaaaaac
    10801 tattagcctc ttctaccttg aaatgggtcc aatctatctc agctggtgtt gatcagctac
    10861 cccttgagac tttttcaaag aaacatattt tactttcaaa tggtagtggc atccacagtg
    10921 aatcgattac ggagcatgtc ttaggaatca tccttgggta ttctcgtggt ctttttcaag
    10981 cacaaaaggc acagagtgac atgaaatggt taggttcatc ggtccattac caatctatcc
    11041 aagggaaaaa aatactaatc attggaactg gccatattgg aaaaatgatt gcaaaaaaat
    11101 gcaaagcatt agacatgaac tgctatggaa tcaatacatc cggtcatcca gcagaaggaa
    11161 tggaaaagac tttttcttta aatcaactaa aggaagttct tcctgaagta gatttcgtag
    11221 tcaatatctt acctctaacg gaggaaacaa ctgggctctt tgatcagcat atgtttgaac
    11281 actttgatcc aaaatctgta ttcatcaatg ttggccgtgg tgcttctgtt aagacacaag
    11341 atctggtgca ggctttaaat aaccagcaac tttcctttgc agcattagac gtttttgagg
    11401 aagagcctct tccacaagat catcctcttt ggaaaatgga caatgtactg attacttccc
    11461 acattgcagg tttgacacct gattttcaaa agaaattaat ggctattttt ttaaccaatc
    11521 tgaaaagcta ctttgccacc catgagcttc aaaccaatca agtaaaattg tctgcaggat
    11581 attaatagct gaaaacaata ttttcaaaag aaaaaagaga aggtgacaaa aattgtgtca
    11641 ccctctcttt ttttattgtt ttttaaagtg ataggttggc tcctccaggt tgggatcata
    11701 atcaacaagt aaatcatatc ctttaaagaa ccattcatct gcttcctcga taaaaaaaag
    11761 tgtcccatca atttccactt tctttacagg atgttctggc tgttcaactg acattccaat
    11821 cgaaaaacca tcatgaacct ctgttttgcc gtagactttt ccaaaaaaac gtattccgta
    11881 agtttctggc agatcaattt ctgagtcaaa ccacttttgt gcttttggcg ttaccgttaa
    11941 tttcatccca acaacctact ttctcttttc tttctattga ttcatcttat cgtctagaat
    12001 atccacatcg cctgtctctc caataaccag tagattatca ttttcacgaa catattcatc
    12061 tgctgcagga gaagcgataa ccgtttgatc tactcttcgt atggcgacta ctgttaagtc
    12121 aaaacgttgc ctgaaattta attcagctaa tgttttgttg aaaaatttag gattggagac
    12181 tcggatttct gctaaagaat atttatctga caactctagg taatctaaaa tattctttga
    12241 cactaaatta tgcgcaatcc ttattcccat gtctcgctcg ggatggacga cgcgatcagc
    12301 tccgatcttt tcaagtactc tggcatgata ctcattttgg gcttttgcta aaatatttgg
    12361 tacgcccatt tcctttacca tcagagtaac taagatactg gcttgaatat cttctccaat
    12421 agcgacaatt acatgatcga aatttcttat gcctaatgat cgaagtgtca tttcatcctg
    12481 tgcatttgcc acaacagcat gagtggctat attcatatat tcattgaccc gatcttcatt
    12541 actatcaata gccagtactt cttgaccagc ttcgatcagt gttcggcaga tacttccgcc
    12601 aaatcgtcca agacctacaa ttgcaaaatt ttgtttcacc gttttcctcc tttagtatct
    12661 tcttttctca gtataacaaa gcctttacaa aaacaaaagc aagcatcatt attgtaaaaa
    12721 agccaagaaa aataatagtt attttctcag cttttgatta tcctctttat catcgttttt
    12781 atttcaccaa accatgtttg aatgcataga tagcggcttg tgtacggtct tccacttcta
    12841 gcttagccaa tatattggaa acatgtgttt ttactgtttt cagtgtaata aacaattcgt
    12901 ccgctatttc ctgattactt tttccttcag aaatcagcat taaaatttca ttttcccgat
    12961 tcgttagttc ttcatgtaat acaggctcat ttcgccgact cattttttcc atcatttttg
    13021 ttgtcacttc tggttctaat actcgctcgc cacgttgcgt tgcccttata gcatcagcaa
    13081 tttcatgtgc ggtagaggtc ttcaacaaat accctgctgc acctgcttca atggcaggat
    13141 aaactttttc gtcatcaata aaactggtta cgataatgat tttagcttct ggccaatctt
    13201 ttaagattgc ttttgttgat tcgataccat ccatttcttc catgactaag tccattaaaa
    13261 tgacgtccgg tcgaagtgtc atcgccttct catagccttg tcgtccattt tcagcttctc
    13321 caattacttc aatgtcttct tgaatagata aataagaaga gacgcctaag cgcaccattt
    13381 catggtcatc tactaataaa acttttatca tccttatcta tgcctccttc atcaaaggga
    13441 ccttgatttc tacacttgta ccttgacctt taaaactaat gatttttact gttccaccaa
    13501 taccagctac tcgttctttg atattgttca atccataact tcccgttttc gtttcattca
    13561 tattaaaccc tgttccgtcg tcaataatac gtaacaaaag attattgtct attttgtgca
    13621 aatatacctc taattcattg gctttagcat gtcttaatgt atttgataac aattcttgaa
    13681 cgattcggaa caagtgatcc tcaatggaac tagatagttt tacatcttct acatcccatt
    13741 tcagcgaaat ctggatttta ttttgcaatt ccttcaataa ctgctcgata ccttgcttta
    13801 aacttttctc ttctaaattg actggacgta gatgaagaag cagcgcacgc atctcagact
    13861 gggaagcgtt gatgatctct gctaccattt tcaactgctt gcgaaacatt tctggtgtct
    13921 cgcttttttc tgcttgctcc gtcaatgccg acatcatcat cattgctgca aacaattgtt
    13981 gcgaaactga atcatgcagc tcacgggcta atcgatgtct ttctaattct aaaatttctt
    14041 ctttggtttg tccatctaca tattggggac ggctcgttac aatttgtaat tcactagata
    14101 cttcaatcat tttttctttt atcttagtga tttccttatc aatatctttg atgtataaat
    14161 cgtccgatgc acttgggatc ggaagaagca acaatttgga ttcataattg ccagaagaaa
    14221 gtgcacgcag cttttcttcg attttcccgt attttgtctt ttgaataagt gataataata
    14281 gaaagacaat taatcctaca cctaaggaaa tggctaacaa atggaagata agcggaacat
    14341 ataataaacg tgcacgcagt aattctcccc accaatgact ttgattgctg gcataaaaat
    14401 aagtgaaaag tgaaaataga ataataagga aagccgcaat ccccgaataa acagctagca
    14461 tcgctctgga tatttttccc atcatattcg tatcacctcg acatctccaa tcaaggtatt
    14521 agtaataatc ttcaatctac gagggttttc atcataatca ctgctgtaga ttttgatttg
    14581 ttcatttttc aaagaaaatt gctcttcttc aaacaagaca tttcctacta atgtcgcatg
    14641 ctctaatcgg atggctacgc ccaaaggtac taagatccgt gttctgccta tcccttttct
    14701 tacaatcaca atattatcat cttttggcaa aagcgtattg cccaaatcta tgatagtatc
    14761 accagaaatg acggcgatgt tgatatcgtc ccattcatag acgtcgtttc caatacgttg
    14821 gttaccgaat aattgctgtc ttttccgctc attattatgt gtttttaatt gctctgtttg
    14881 gatcatcatg atctgtttct ttcgccaaaa agcatttttg gttaaatcta tccctgaaat
    14941 ctctacacct ttcagaccaa taaataaaac ggcaaataca gccatcatcc ataaagcagg
    15001 actgttgact aagctaaaaa aaatgaccaa acttcctaat atcaattgaa aattttgaaa
    15061 tttagtacga ggatgtttgc gcatagctag atatatattg aaaataccga atatcactaa
    15121 aagcaaaagt ccagtattgt ttacgatttg ccaaacagca aataacagca gtagtgcttc
    15181 aactaccaca aaaaaacgcc aagaactatt catcgctctt tctccctcct gacacattct
    15241 catttcatca tacctatttt tttaactgtt ggctatagga ctttagatgt aagatcgttt
    15301 gttgaaatct aaaatacaca aagagaaaaa gatctagagt gtatcatcag taagatgaac
    15361 tctctagatc tttcagtcag actacgcttg ttctactttg acgattttaa ctaacatatc
    15421 gccacctgga gtagcaatag ttacttcatc atttttacgt ttgccaatca atgcttgcgc
    15481 aataggagaa tcattggaaa ttttgccaga aaacggatct gcttcagcgc ttccgacaat
    15541 cgtgtactct tcttcttctc catctggtag ttcgatgaat gtaactgttt taccaattga
    15601 tacctcatct gcatctacac cgtcgttatc gatgatttga gcaaaacgaa tcatattttc
    15661 aattgttgtg attcttcctt caacaaatgc ttgttcatct ttcgctgatt catattcaga
    15721 gttttcggaa agatctccga aaccgcgggc aattttgatt cgttcaatga tttctccgcg
    15781 tttgactgtt tttagttctt ctaattcttt ttctaatttt tctttacctt ctagagtcat
    15841 tggataaact ttctctacca tgtgtaacgt ctcctttttt cgataaaata ccgcaaaagg
    15901 taatcttttg aggaatatag tccaaaaaag attaccacgc ttttcttcaa atgtaaatgt
    15961 ttttctaaaa atgatcaatg tctagagcct gtttttctaa gaattattta catatttttc
    16021 cactaattcc atatgttctt cataagtttt agcgaaataa acatttccag tgctcgtatc
    16081 tgctacgaaa taataatagt cattttctgc tggttctaat actgccttga ttgcctcttc
    16141 gcttggattg tcaaaaggtc caggtccata gccggtattg gtatacaaat tatatggtga
    16201 atctacagct gtatcttcat acgtcactaa ttctttgtgt tcacctaatg cgtataaaac
    16261 agagatatct gactgaagtg gcatatttgc tttgattcgg ttaaagaaga cttgtgcaat
    16321 gtttttacga tcattttctt ttacgccttc tttttcaact agagaagcta gagttaaaac
    16381 ttcttgtaca gtcaaatttt tctgcttgat agctgaataa tagttttgca tgaccgtatt
    16441 cgtcttgttc accatttcaa tcactagatc cttaagcgaa gtactcgtgt aataatcata
    16501 tgtggctggg aataagtaac cttccaaacg gtattttgtg ttctcagctt tagatgcact
    16561 ttccaacagt tctgggaatt tttggtgcaa ctcgttaaag aaagtctcat cattcactaa
    16621 atctaaaaat tctttcttgt ctttaccagt taccttcgct acccgttcag caatctgatc
    16681 gatatcgtac ccttctggaa tggcaatttt cccatctgca acttttgttg gctcagaagt
    16741 accgccttct tgcaactgtt tgccaatctc gtcaagagtc atattaggcg ctaactgata
    16801 atagccagct tgaaagccag ttaaattttt aaattttgta taataattga aaacaatccc
    16861 gcttttaata atattgtcct tctccaaaat ctcaccaatc tgcttgttag aagagccact
    16921 cgggatctcg acttggacta attggtcatc tgatttatct aaaggcttca acccgctatc
    16981 tacataacga tacacggtaa atccaagaaa tccgccaata atcaataatg taagagcaat
    17041 cactaaaatg atcttcctca caatcttatc ttctttttta cgcagatttc tccgcttgtc
    17101 gattatatct gctgtagctg ccttactggc ttctttttta tctttacttg ggtgttgttt
    17161 gtttcctgac aaaacaactc ctccttacta tcaagttcgt aactttattc attatacatg
    17221 atatttgaat tggcgtgaat gattttattt aaatattcta tcaaaaatgt aagtagtaaa
    17281 tccattacta taagattaaa ataaatgtta tcaagataca agctatttta gctttattct
    17341 ttctgtaaaa aagaaaggag actaaataaa ctattatgaa aaaaggaaaa catgctaaaa
    17401 aaagaaaata ttcattttta atgatcgttg tcgtgttgtt tagccttagt gcgttctcta
    17461 caggaactat tcttggatgg caattttaca ataatgaaca ctctcagata gttgcccatg
    17521 ctcaatcagc tcaaaaaaag ttgatactga ctaccaatga acaacagctc gttgaaaaaa
    17581 tcgatcaact attaagaaaa aatgattata ttggctcaat ttacgtaaag aaaaataatc
    17641 gcatcatcat ggaaaaaggc tatggctatg cgaataaact cacgcaacaa ccaaatgatc
    17701 cttctcttta ttatcagatt ggttcggttc aaaaagcatt gactactctt ttgattttaa
    17761 aacaagtcga gcgtgggcac ctttctttag atactaagct ggctcaattc tatccacaaa
    17821 ttgctggcag ccagcaaatt actatccgtg acttgctgta tatgcgctca ggcttgcacc
    17881 gaactgcttc tccaaccata ccgatgactg atgaagaagt cgttcaattt gcgattcagc
    17941 acttaaaatt tacagattat cagacctctc attatgattc attgaacttt accatattga
    18001 ctggaatttt gatcaaatta acaaatcaac cgtatgaaaa attattgacc gatgatatca
    18061 tcgaaccttt aggattacag catacaaaat tttatgacca agtcaaaaat tcatccaatc
    18121 atgcactatc ctatcaaatg tctaaagagg atgattactt taaaccactg acagaatctg
    18181 aaacagagat ccataacgaa ttagggactg gtaatatcag tatgtccgtc tatgatttaa
    18241 atacgctgtt tacacaagtt ttatctggaa aactagtttc caaagactta ctcttctcat
    18301 tatggcgtga aaatgcgaat ggacatccat atagtggtgg ggtatatagc ggagataatt
    18361 atatcctgtc gcaaggaaat atcgactctt ttgatacatc tgctatattt agcaaagatt
    18421 cacaggatgc tatcatcatg gaaagcaata taaaagctga caaaactatc aaactgccta
    18481 ctgctgatct aagaaaccaa atctatgacc tgatggaagg aactactaag ttggcaaata
    18541 gctgatatga tgaaaagata gcaaatacta cttctttgaa tagcatttgc ttaaaaaaag
    18601 tactcgacta aacgtaggaa agagtgtggg acaaatctaa aaatcaattt ttgctacaga
    18661 ctttaattcc aaataaacag aacagaaaga ggtgttcttc tttctgttct gtccaaatta
    18721 ttctaagatt tctctttacc aacattattt gacaaagggc ctattaaatc taaaatagta
    18781 tctttagatt gctaaaatat gtgctagcaa atcattcatg gcttgttctt atttttaatt
    18841 ttatgcaata aaaaagaaga acaattagcg tgattataga aataaataat gagattttag
    18901 aaaaaaatgt gtatgaatac ctgattgtta ctttatttat tccactttca ctttttactg
    18961 taacaaaagg acctccttgt tttagtactg tagcattttt tgaattaaca gtaaccgtta
    19021 atcctttata ccggaataca ggtaaaacta ttgttttgcc ttttgaagat ttaaacgtaa
    19081 atgtaatttc atctttattt ggagaaaagc tactataaat aacttgacca tcttctgtaa
    19141 cttgatggtt tttcaaaagc aattgatctt ttttatagcc tataggtaca taatcaaaat
    19201 tctcaaattc ttcaagttta ttttccattt gttcattaga ataagttcct tgaaaattca
    19261 tttctttcac catgttaact actgatgaat aatttaaaat tgaaatagct aatattaata
    19321 atagtataaa atttcttctc cttgtataag aaaatttttc caactgtgga gaaataaata
    19381 gagatactga aaaagatatg aacaatgttg aaaatatcat aaaacgccaa ggagcttgaa
    19441 taatatttaa aggagtattt tgtaaggcaa accatggaaa tatttttgta caaagtagaa
    19501 tagatattat acccattata taagtgattt tagataagat tcctaatttt ttaaagttaa
    19561 atgttagtat aattaaacaa ataagcaaag ttaagccaat accaaatgat agtaattctg
    19621 aatctatact tccaatcaaa aactctttac ccagataaat tgctcttttt tgtaatatat
    19681 ccacataagg tctattcaga tcttgataca atgtctgctc aatcatagga cctataaacg
    19741 ctagtgaaag aagtaaagta actatagcag cttttattaa tttaataatt aagaagattt
    19801 ctattttctt tttccgataa aattgaataa ttaaagcaat tataatcatt accatagtca
    19861 tagctacaga aagaacatga gtgtaaatta ataaagtcat gctaatcgcc aacataaccc
    19921 aagattcttt ttttgagtcg taaagcttta taaacccgag taaaataaga ggaagtattg
    19981 acattgcgag taattccccc attgcccctc gaagaaatat ttctacactc ctaaaagaag
    20041 atgttgtata tataatagcg aaaaaggagc tagtaacatg ttttttggta atttcataca
    20101 ttacataatg acatataaat agagtaatga gcgtaacaat ataaagaaaa aggttatagc
    20161 ctattgctaa attagtaaac ttgatcaata aaaataaagg atatagagtt aaccatgggt
    20221 aaaaattatt tacttgcatg ccatatgatc taaacgtttc aaaattaata ggggagctaa
    20281 aaatagattc taaacttaac gcacgattaa tgtgaaatgc cgtatcataa aagccaaata
    20341 tttccatgtt tttaaatgct ggaaataaat ataataaact aacaaatgca taaactataa
    20401 atatttttac agaaaaaatt tttttcaaat tcatatacca cctcatgttt ttttatatta
    20461 catatagtat catttatttt tttaaaacac tagattagtc actatataaa atgtgattaa
    20521 ttttattatt aataaaatat tttttatatt acaaaagtag tgtttc
//
LOCUS       DACEDD010000050        20248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEDD010000050 DACEDD010000000
VERSION     DACEDD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.050"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            193..2235
                     /locus_tag="IWO45_002095"
     CDS             193..2235
                     /locus_tag="IWO45_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0333565.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2246..2866
                     /locus_tag="IWO45_002096"
     CDS             2246..2866
                     /locus_tag="IWO45_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0333566.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2878..3336
                     /locus_tag="IWO45_002097"
     CDS             2878..3336
                     /locus_tag="IWO45_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0333567.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3355..3633
                     /locus_tag="IWO45_002098"
     CDS             3355..3633
                     /locus_tag="IWO45_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0333568.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3658..5025
                     /locus_tag="IWO45_002099"
     CDS             3658..5025
                     /locus_tag="IWO45_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0333569.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5040..6185
                     /gene="dgoD"
                     /locus_tag="IWO45_002100"
     CDS             5040..6185
                     /gene="dgoD"
                     /locus_tag="IWO45_002100"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ0333570.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6212..6703
                     /locus_tag="IWO45_002101"
     CDS             6212..6703
                     /locus_tag="IWO45_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333571.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7146..8345
                     /locus_tag="IWO45_002102"
     CDS             7146..8345
                     /locus_tag="IWO45_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0333572.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGGIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8840..9598)
                     /locus_tag="IWO45_002103"
     CDS             complement(8840..9598)
                     /locus_tag="IWO45_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0333573.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9766..10701
                     /gene="pfkB"
                     /locus_tag="IWO45_002104"
     CDS             9766..10701
                     /gene="pfkB"
                     /locus_tag="IWO45_002104"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0333574.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10698..12143
                     /locus_tag="IWO45_002105"
     CDS             10698..12143
                     /locus_tag="IWO45_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0333575.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12171..12626
                     /locus_tag="IWO45_002106"
     CDS             12171..12626
                     /locus_tag="IWO45_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0333576.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12645..13619
                     /gene="lacD"
                     /locus_tag="IWO45_002107"
     CDS             12645..13619
                     /gene="lacD"
                     /locus_tag="IWO45_002107"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0333577.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13730..14291)
                     /locus_tag="IWO45_002108"
                     /pseudo
     CDS             complement(13730..14291)
                     /locus_tag="IWO45_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14435..15049
                     /locus_tag="IWO45_002109"
     CDS             14435..15049
                     /locus_tag="IWO45_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0333578.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15290..15673
                     /locus_tag="IWO45_002110"
     CDS             15290..15673
                     /locus_tag="IWO45_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0333579.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16036..16545
                     /locus_tag="IWO45_002111"
     CDS             16036..16545
                     /locus_tag="IWO45_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0333580.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16645..17295
                     /locus_tag="IWO45_002112"
     CDS             16645..17295
                     /locus_tag="IWO45_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333581.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17746..18684
                     /locus_tag="IWO45_002113"
     CDS             17746..18684
                     /locus_tag="IWO45_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0333582.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18697..19749
                     /locus_tag="IWO45_002114"
     CDS             18697..19749
                     /locus_tag="IWO45_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0333583.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19807..>20248
                     /locus_tag="IWO45_002115"
     CDS             19807..>20248
                     /locus_tag="IWO45_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0333584.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
BASE COUNT         6416 a         3216 c         4278 g         6338 t
ORIGIN      
        1 cgaatattta atattcacat tagccctaaa ataattgtca taccatgtta catttgatat
       61 aacagcgttc attttaaaaa tagctgttag tgtgacaaaa cattaaacca agtgtgacaa
      121 aaaacgtgat gaaagctttc cggaaacgta tgtgaacaag tatataatga ttttgtcagg
      181 aggatagtca aaatgaataa acgaattcgt gaaatactca aactgattat taaaaaccct
      241 gaaatgaaac tttccacttt aacaagtgaa cttgatttga cacgtcgtca aatcaattac
      301 gcgatcaatc aatttaatga agaattgaaa acaaagaaca ttccgttaat tcagagaagt
      361 cattctggaa atattacggt accaatcgag gttatccaaa tgatatcaca gcctgattac
      421 gacataggtg atgatcagga acttgtcgtt ctcactgata gtgaaagaca agcattgata
      481 gtgatgactc tgatcgctaa tatcgattac atcagtctgg atcatttggt agatgcggtt
      541 gatgttagta agacgactgt tatggaagat atcaaacgaa cggaaccgtt catcagaaat
      601 tatagtctga caattcgata taatcggatg aatggttacc aattggttgg ttcggaacac
      661 cgtatcttac agctgttatc ggatctagta aaacggtttc cgatttttcg ccgagaaatg
      721 attcgcagaa aattggcaca agatgtttct gaagaagaaa tcgtccattt gatccataac
      781 atggaacaaa tgttgcatct tagttattct gatgagtcgg tagactattt gcagacatca
      841 gcgagatttt tagtaagccg tgggatcaag atgtcagata agcacgcgtt ttttcccgat
      901 gatgtgacgg atactcctga attccgaatg ctgatgatct tggtacatga aacaggttgg
      961 agtctatcca aaagttatat ggagtggttc acgttattat ttttaacttc caatattttt
     1021 gagagcaaaa ctactcaaaa ttatgattcg gatcaagaat tgcgaaaatt gatcagccaa
     1081 atggttgaaa atttccaaaa tcaaacgttg attttgatcg atgaccgaga agactttgaa
     1141 cgtaggatac tgagtcatct gcgtccagcc tgttttcgaa tacgctataa cttgagttta
     1201 ggagtttatt caatagatag tctgatcaag gatagtaatc atgcgatttt gattgatttg
     1261 atgaaagaat tgattattcc tattgagaat tggctaaata aggcctttcc aaatgatgaa
     1321 ttagatcttt tatcctatta ctttggctat caattaagta atcataataa attgaataag
     1381 caacgacaaa gggctgtagt tgtttgtact aatggtgtca tggtctcaaa gttagtacga
     1441 gccaatatgg aaaaactttt tccagagatc cattttttag cttccctatc tgtgcgtgat
     1501 ttctacaaat ttgaagctga ttatgatctg gtctttacta cgacgccact aaaaagttct
     1561 atgctgcagt ttatgatcga gccaatcatg acatatcagc aacaaatcag tttacgatat
     1621 cgagttttga gtgatttagg aattactaag gttgatcgag caatggatga attgttaaca
     1681 atcattagga aatatgcaaa tatcaccaat ccagtttcgt tgcgagaaga actgcagtac
     1741 tttttgatca aggaagatgg aagcacgccg ctggacaact tcaatgtatt gccttcattg
     1801 acttattact tgcggccgag ttatgtacaa atcgttgaag aagagatgtc ttgggaacga
     1861 gcagtggagg tggcttgccg accgttattg gaacatcaaa tcattgactc gcgttttatc
     1921 gctgattgct tggatcaaat cagacaaccg ggatatgcag gttacttggg aatgaagact
     1981 tgcattcccc atacaacggt cgataaaggt gtaatcagtg acggtgtcag cattttagtt
     2041 tgtaaaaagc cgatacgatt tcctaatggt gaagatgtca gcttgatttt gccgctttca
     2101 ttttttgatt taacgaagca tctgcgagcc atcaatcaaa tcgctgatat cgccaaagat
     2161 gattcattga ttgagcagtt attgattgct gatgacgaaa agacagttta tcaactatta
     2221 agacaaagat cttaaggagg aaaacatgag actagcagat tatcctaaat tgacagtgat
     2281 catgcgtggt tatacttttg atcaggcaga tgctatttta caagcaatgc aggaatttga
     2341 ccatcaattt gccgtagaga tgacaatgaa cacaaaagga gctcttgaaa atatacatga
     2401 actaaatcaa cgttatggtg acacgactta tatcggcgct gggacggtaa ggacattaga
     2461 acaagcccaa gcctgctatg aggctggtgc aaaattttta ttaggaccac atatgttcac
     2521 aaaagaaatg ttgtcttacg ccaataagaa aggattattg gcggtacctg ccgcaatgac
     2581 tccttcagaa gtcgaccgta tgtttaaaga aggagctgat attgtaaaag tatttcctgc
     2641 agcggttgtt actccacgtt ttttcaagga tattcaagca cctttaggta agttaccact
     2701 aatgggggtt ggtggaatca gtaaggagaa tgcgaaagag ttttttgaac aaggtgctag
     2761 ctatttaggt cttggttccg gtatgttcaa caagcaagac atcgaagagc taaatgttaa
     2821 aaacttagca cgttcgatga aagaattaat tgaggctatt aaataggagt gaggactttg
     2881 gaactttatt tgaatgagga actcgttttt cgaaatgttc aagcaaaaac agatagtgaa
     2941 gtcttagcgt ttttagcaag cgagatgtac aaaaaaaact acgtgaagga ggaatatatt
     3001 caagcaatcc aagaacggga aaaagaatac cctactggat taccatcaac accaccagga
     3061 atcgcaattc cccatgcaaa ctatgaaatg gtcaataaaa caactttagc aattgcgact
     3121 cttaaagatc cggtcctatt tcataatatg gaagataaca atgagcagtt accaatccaa
     3181 attgtgatca tgatggcgat tggggaacca catgggcaag ttgaaatgtt acaaaaaatt
     3241 gttggaatca tacaagatga gccactgcgg caagaaatga tcagagcagg caatgatact
     3301 gaattattag aactattaaa gaaagcggtt ttttaatata aggaggaaat cataatgaaa
     3361 cgaattttag tagcatgcgg aaatggtatc gccacttcaa cagtagtagc aacaaaagtt
     3421 agagagtatt tgcaacaaca tggcattgat gtttcaacga ctcaaacaaa attgatggaa
     3481 gttccaggaa aagttcaaga ctatgatcta ttggtgacga ctggtcaatt tgatggtcaa
     3541 acaggtggtg taccagtaat caaagggatg ccgattttaa caggtatcgg tgccgatcag
     3601 acaatggaag agattttgaa cttactgaaa taatagctga tcaaaggagc tgaaaaaatg
     3661 aaaatcatta tgaacgtctt taacttcttt attgatgctg gaccaacagt tatgttgcca
     3721 gtcatcatca cgattattgg tttgattttt gggttaaaga tttctcgtgc ttttaaatca
     3781 ggtttgacat taggtatcgg ttttgccggg atcaagttga tattggactt tatgacgacc
     3841 aatgttggac cagctgccaa ggcgatggtt gatcgaactg gtgttaaact agatgctttg
     3901 gatgttggct ggggttcgat tgcggctgtt acttgggcat cgccaatcat tccaatcttg
     3961 atttttgcca ttctattggt caatatcgtt ttgctgattt tgaaacgtac acatacgctg
     4021 gatgttgata tctggaacta tcaccatatg gcaatcgttg gtgttatagt gtattttgtg
     4081 actaagaatg tcttcttagg tgtaggtgca tcagtagtga tggcaattgc gacttttaag
     4141 atttcagact ggtcgcaacc gatggtagaa agtttcttcg gaatccctgg tgtttcttta
     4201 ccaactgttt ctgcgctttc atcactggtt attgcttggc cgttaaattg gctgctggat
     4261 cgtatcccgt tattcagaaa atcaaaattc accatcaaag atgcacaaaa atatttggga
     4321 ttttttggtg attctatgat catggggtta gttattggga tggtgattgg tgttttagcc
     4381 ggctatgata tcaaagcggt tttacaatta ggtgtcagca tgtcagctgt tttggttttg
     4441 attcctaaga tgacttcatt atttatggaa ggattgatgc caatttcaga tgctgcacaa
     4501 aaatggtcac aaaaaaaatt caaaggtaca cgtttattta tcggattgga tgctgcagtt
     4561 gttgtaggta atccagatgt tatcacaaca gcattgatca ttattccact aacgattgca
     4621 ttagctttag tattgccagg aaaccgtgtg ttaccattcg cagatttagc ggttgttcct
     4681 ttccgcgtgg caatggtcgt tgccttgact cgtggtaatt tattaaaaaa tatcattatt
     4741 ggcttagttg tgacagcttc attgctatgg tgtgggtcag ctactgcgcc aatgctgaca
     4801 gcaattgcca aatcggttgg tattaactta ggaacttctt caatgttaat ctcatcattt
     4861 gctgcgactt ccatgatcca aagttatttg gtatttatcg catttgctta caaaccaatc
     4921 atcggtattc cagtgttgtt attagccttt gcgggcgcat ggtattactt cgatggtatc
     4981 aaaaaagtta atcatgaaat ggatgaacca ttgaaggcac aatagtagga gggtttaaat
     5041 tgaaaatttc agatgtcaca gtgtataaag taaaaccaag atggattttt gttaagatct
     5101 cgactgacga aggaatcgaa ggctggggag aaatgatctc aggaactaaa acagaaacgg
     5161 ttgttgctgg agcttatgag ataggaaacc ggctgattgg acgtaatccg tttgaaatcg
     5221 agcgcctctt ccaagaaatg catcgatctt tcttccgagg cggtcctatt aatggaacca
     5281 tcgtttcggg tttagaaatg gctctgtggg atatcaaagg aaaagcgttt aatgttccgg
     5341 tttatgaatt attgggcggt gcagcacgtg ataaaatcaa ggtttattct tggattggtg
     5401 gagatcgacc aagtgaagtt gcagaacaag cacaagatcg gtttgatcgg ggctttacgg
     5461 ccgtcaagat gaacgcgaca gaagagctgc attatatcga ttcctaccaa aaagttgatg
     5521 aagtggtgga acgagttgcg tctattcgcg aacgttttgg tgatgcaatg gatattggtg
     5581 ttgattttca tggacgggtc cataaaccga tggcaaaagt gctagcaaaa gctttggagc
     5641 cattccatcc aatgtttttg gaagaagtcg ttttacctga aaatgaagaa cactttaaag
     5701 aagtagctga ttctgtggct gtaccattag ctactggtga acgcttgtac actcgttggc
     5761 aattcaagaa cctctttaaa caaggtactg ttgatattat ccaaccagat gtggctcttt
     5821 gtgggggaat cttggaaacc cgtaaaatcg cagcgatggc ggaagcttac gacatggctg
     5881 ttgcgcctca tgcgccatat ggtcctgttg cactagcagc aactctgcaa gtagactcat
     5941 gtacgccaaa cgtctttatc caagaacaaa gcttagggat ccactataac aaaggttttg
     6001 atctgttaga ttttgtgaag aacaaagaag ttttccaata taaagacggc tacgtagatc
     6061 taccttcaaa accaggtctg ggcttggaaa tggatgaaga caaaatcaaa gaaattgcac
     6121 aagaaggttt gatttggacc aatccgcaat ggaaaaatta tgacggtacg atcgctgagt
     6181 ggtgataaat gaaaataaag gatgatgaaa gatggcagaa gcacttatga tcacagtttt
     6241 tttaggactg ctgattattt tgcaaataat cgatcaaaag aaaattatcg tgtttgctca
     6301 gagtagtcct gtaaggaaga gtttgagtat tttactgaca gtcgccatcc tgttgatttt
     6361 ctggccggaa catctgactg atcagattaa actggttgct tttgcttctg tcattttgat
     6421 caatggtttt ttgaaggagg gtctagcaaa agattctgtt gttagatttg gagtattgaa
     6481 cggtaacttt aatcgatatc ctcaagtcca agtagaaaaa ctaggtgacg ataaatcatt
     6541 tatttcattt tacaaaagca agaataattc tttttcaatg atttttgata aagatcaaac
     6601 tgaaatgatg gatgtctttc aagaacttga tatccattcc aaaattactt tagatcaaag
     6661 ttcggaagaa aaaagagcag ttgtaaaaaa agataatgaa tgattctact accgaaatta
     6721 tgatttcttt atcacgaata atgaaacttg ttttaatgaa tcatattgat tgaagtatac
     6781 aatgtatatt aagttttgga aagaaatata ttggtacaag atatagcagg aatcgattac
     6841 tcgatccctg ctttttttaa cttcctttgc agcaaaaaaa gctcgtaatc taagagatga
     6901 tggatagttt ttagaatata tatattttct tcaattccag aaaaaaatga ccaagctagt
     6961 atgctaaaaa agtacgatgc ttctatgatc ttttttccgt ctttattaac agatgtttta
     7021 attgatcaac gcatattgta atctacccta atttccatag ttcttcacca cttttaaaaa
     7081 tagtaaaacc cctacaagta tgctatagta aattgctaaa gaaaaaccaa taaggtggga
     7141 gatgtgtgcg cgatcaaaca agcggaaaaa tgattcgttt ttattataca agtttgcatt
     7201 ttctttattg gatgatgttt tgttctgtct atgggtttgc gacattgttt ttgatttatt
     7261 tcaagattga tccagctaaa atcggaggga tcttagcatt ggtcaatatt gtttcaacgg
     7321 tgcttcagcc ttttgtcagt caagtgatca tcaagaaaag aaagatccca ctgatcaccg
     7381 tgctgaagac gatgatagca ggattaggag tgattctctt agctggggta ttgattccga
     7441 atttgccgat cattacttat atcttgggtg ggattttagt agtttctatg caatcatttc
     7501 tcaatgcgct ggcatttgag tattcaaata ccggctacag gatcaacttt ggcttttcaa
     7561 gagctggtgg ttcattttct tatgctgtaa cttctttttt actgggacag ctgttagcga
     7621 tttattctgc cgcgattttg ccaatcttgg ttatagttga agcagtgata tttttcgtta
     7681 ttttgcatct gctgccggat gtaaaaaatc aagaagtttt tcctatgaca aaagaacagg
     7741 atcgctcagt ttctttgaga aaaaaatatc ctttcctgat tttgttattt ttaggtttca
     7801 gttttctttt tactttccac accatgatca acagcttttt ggcacagatc tttgaaaata
     7861 tccacggcgg cagtaaagaa gttggcatcg cattgatgat cgccgcttta tgcgaattac
     7921 cgggaatgat cttttttgaa cagcttgtca aaaagaaatc cagcaaattt tggctgtctg
     7981 ttgccagtat ttcatttgct gtcagaggat tcatgatgct gctggccagt tcgatcctga
     8041 tgatggaaat gacgcatctc ctacaagcct tcagtttcgc tttgtttatt ccagcttcgg
     8101 cttatttgtt caatcaattt ctggcggatg atgatcgtgt atttggacaa acgatgatca
     8161 tcatcgccac aactcttggt ggggggatag gaaatgtctt gggcggcagt ctgctgcagc
     8221 atttcggtgt atgggagatg ctgattttcg gtactggttt tgcggtgcta ggtgctttat
     8281 taactatttt gggcattcga aaaataccga agacaggaaa cgatagtgat tctcttgttg
     8341 agtagcataa aaatacttag atactagttt tatataaata acatatagtg attattggcg
     8401 taattacaaa aaacgagaat aacttaagta agcatttcta aataattgag aaacctcatc
     8461 taaaaagata agaaaatatc cctaataaaa cttctgtaac attttagata actgattgta
     8521 atctgaataa taaactataa ggagacggtt agacggtctc ctttttttgc gtattataat
     8581 tctagaattg ttggaaattg ataataatta acaagaaata ttagacagga acttcgaact
     8641 gcttatcaca ttcttattat tatatatttg cgtaagtagt cctggtagtc tcaatgcact
     8701 attctatctt gaaactaaga agttatagaa ggagcaatga tagaaagcac gagaatacaa
     8761 atagcgatta aatatttttc tagacaacct tagttaattc acacagatat attttaggta
     8821 cagtcttaca gatatagagt tagtgctgtg tgaatcctga tgttcgcagt gcataggagt
     8881 caagtaagat tctagccagt atagaaagtg gaagtctgga gcgtacctca taatctggga
     8941 agaaggagcc ttctgattta aagccaacaa aaggaatctc gatatgttcc atcaaacttg
     9001 aatgacgatt agcagtaagc aaaattgtcc cgatctcagc tttataacag ttttcagctg
     9061 cggtagtcaa ttcctttgtt tcaccgttta gtgaaacaaa gatcacgatg tcatctttgg
     9121 ataattttct actaatgttt tttatgatat tgggatcagt atgcatctca caatatttac
     9181 cggtaagttg aaatttgacc atcatttcct gagcaatcaa ttctgaaaaa cctcgggcaa
     9241 aaatcatgat tcttctagca gccttgattc gttgaatagc atcttcaata ttcccgcttt
     9301 ctaccatatt gatggtgcgg attacctctt gctcattttt aagaatagca gtctttattc
     9361 cttcatcaac tttatcaaga gcggcaaaat tgatatttgt attttctttt tcttttaacg
     9421 aatgtttaaa agacgtaaac ccctcatatc cttttttctt catagtacga acgacggttg
     9481 ctgttgaaac gttggcatat tcacttagtt ttacaatgga ataggagggg atttcgagta
     9541 tgtgactttg aataaaatcc cagagatatt tctccgattc acttaatttt gtatccatat
     9601 tttgcctccg attagttagg ataattctat tgtaaagcgg gagtttcctt tacttcaagt
     9661 ttatttaata tattttgtaa aagttttcaa tgaatttagt catttatgaa aacatttaca
     9721 ttcgatcttg atacataatg aatatgtaag aaaggaggag cgtaaatgtc agtttataca
     9781 tgtactttaa acttggcaat cgaccttttt atcgaaacag atgaaatgaa accatcccaa
     9841 gtcaatcgaa caaacgatga tgatatacaa gctaatggaa aaggtgtgaa tgtttcattg
     9901 atcatgaagc aattaggtat cgatagtaca gctttaggat ttaaagcagg ttttaccggg
     9961 aattatattg aggattattt gcatcaaaaa gatattagca ctgattttat tagcgtaccg
    10021 ggaatgacta gaatcaacgt ttttacacaa gttaataaaa ccaatgaaga attcaaattg
    10081 gttaataaag gacctgagat tcctcaggaa caaatcaaaa cttttctaaa tcaaatacaa
    10141 aagctgcaag ctgacgatta tttgtgtgtt tctggcagtt tgccgcgagg ggtctcaccc
    10201 gaaattctta ttgatatcag tcgtatttgt tttgaaaata aggtccatct gattattgat
    10261 tcaagctatc cggaaataat tgattgtctg ccgtatcagc cgttcttatt aaaaccaaac
    10321 gaggaggaat tgcaatcttg gtataatgtc gagcttagtg atcgtgaagg atatattcat
    10381 tatgctgaac gcttgattgc cgatggagcg aaaaatgttc tcctttctct aggaggagag
    10441 ggaggaatat ttttaaataa ggatatcgtt tattctggga attctcctaa aggtaaagta
    10501 gtcaatacag cgtgtgctgg tgacactttg ttgggaacat ttcttgcagg atatttaaaa
    10561 ggtgaacagg tagatattaa tttgaagaaa agtttagcag ctggaagttc aaccgctttt
    10621 cgtaaaggat taacagattt ttcggatgtt gcagagttgg aaaaacaaat cgaaatcaaa
    10681 gagggagcga gtaaaaaatg acaacttaca aaattgttgc cgcaacagga tgtccaacag
    10741 gaattgccca cacttatatg gctcaagaag cattggagca ggcagctaag cgcaaagggg
    10801 tatctatcaa agttgaaacc catggtcaag tagggataga gaatgaatta acaccagaag
    10861 aaataaaaaa cgctgacgct gtaatcattg cagctgacaa agatgttcaa gcagaacgtt
    10921 tcgcaggtaa gcgtgtaatc gatgtgcctg tcagcaaggg gattaaagat gctgaccaat
    10981 tgatcgaaga tgcgttggct ggtaaaggtt caatcactgc agaaaacaaa acagttgacg
    11041 ctgctggtga gaaagcggag atgggggttg cagcaggcat tggtcacagt atttataaaa
    11101 atctcatgaa cggtgtttct catatgctgc cgtttgtagt ggcaggtggg gtaattattg
    11161 caatttcgtt tgccatctgg ggtgtgtact cttttgatcc caatgatcct acttacaatc
    11221 cgactgccgc tatgttaaag ggtgttggtg atgcggcaat gggaatgatg gttcctattt
    11281 tagcagctta tatcgctgaa ggaatcgcta aacgtcctgg tttggttgtt gggtttgttg
    11341 gcggactgtt agccaattct ggcggagccg gtttcttagg cggaattttt gcaggtttct
    11401 tagcaggcta ctttatctta ctattgcaaa aggtcatgaa aggcttgcct aaacaattgg
    11461 atggtctgaa ggcgatcttc ttatatccgg tgatcggtgt cttgtttgtt ggtgtagtca
    11521 tgtctttatt agcaaatcca atggaagcag tcaaccaagg aatgatgaat ttcttggcag
    11581 gattccaaaa ttctaatcca ttaattttag ggattatcgt tggctgtatg tcagcatttg
    11641 atatgggagg tccagtcaat aaagcggctt atgttacagg gactgcgttg ttggcacaag
    11701 gtaaccatac atttatggca ggggtttcgg cagcttgtat cgcgccccca ttgattactg
    11761 gatttgccgc actgtttttt ggaaaatatt tcgacacgaa tgatcgcaac gctggtttag
    11821 tcaactttat cttgggctca acgcacatta ccgaaggagc aattccattt gcggctaaag
    11881 atccgctgcg tgttctgcca actatgatgc taggttcatc aattgctgct gtactgacat
    11941 atatgtttgg tgtccaagtt cccgcacctc atggaggttt tctagtatta cctgttgtta
    12001 ctggtaaggt tgcatgggta gggtcgattt tacttggttc aatcgttggc ggagtaattt
    12061 tagggttgat ccaaaaacgt agtgtagaaa aacgtgaagc agaagcagct gctgttgaaa
    12121 tgaaagaagc aaccaacaac taaaggttat tctagaaaga aggatttcaa atgactttga
    12181 tcaaaacaaa taaagttttt atgaataaaa attttacgac gcaagatgag gtaatgaact
    12241 tccttgcaga tcagtcaatt gtcttgagca ttagtgaaga ccgagatgca gtttacgaaa
    12301 aattgatgga aagagaagaa gaaagcacga ctggtatgat ggatggtttt gccatccctc
    12361 atgccaaaga tgctagtatc aaggatgccg atattgtgat tgttcggtta aacggtaagg
    12421 tcgattggaa aagtttagat ggagaaggaa cggattttgt aatcgctctg tttattccag
    12481 attcagaggc tggaacaacc catctaaaat ttttatctca ggtagccaga cttttgatgc
    12541 acaaagatgt gacagaaggt ctgaaacaag cccagtctgc tgaagaaata gctgaacttt
    12601 taaaccaaaa attagaagaa aactaaaaag gaagtagtga aatcatgcct ttaacagaaa
    12661 aaaagaaaac ttatattcgt caactaagta atgaaagtgg catcatcagt gcgttagcaa
    12721 tcgatcaacg tggtgccttg aaaaaaatga tcggtaaatt tcaaaataaa tcagcaaccg
    12781 atgaacaaat catcaatttc aaaaaaatca tttcttctga attgactcca tatgcttcag
    12841 caatcttatt agatccagaa tatgggttgc cggcagccga agttcgcgat aaaaatgctg
    12901 gtctgttgtt ggcctatgaa aaaactggtt acgatgcttc aactccagga cgtttaccgg
    12961 atattttatc tatttggtct gtaaaacgtc taaaggagca aggggccgat gcttgtaagt
    13021 tcttgttata ctacgatgta gatgaatctg atgacatcaa tgaccaaaag aaggcgttta
    13081 tggagcggat tggttctgaa tgtgttgctg aagatttgcc atttttctta gaattggttt
    13141 cttatgatgc caacgatcct gatacttctt cgaaggaata tgccttgaaa aaaccgcata
    13201 aagtaaatga aatgatgaag gaattttcta agcctcgcta tggagttgac gttttgaaag
    13261 tggaagttcc tgttaatatg aactatgtgg atggttacac taaaaatgaa gttgcttata
    13321 ccaaagaaga agctgctgct tatttcaaag aacaatccga gactaccaac ctgccattta
    13381 ttttcttgag cgcaggagta actgctgaat tgttccaaga aaccttgaaa tttgcaaaag
    13441 aggctggctc tacattcaat ggggtactct gtgggcgcgc aacttgggcg aatggtgtag
    13501 aaccattcgt tactaaaggt gaatatgctg ccaaagaatg gatgcagaca caaggacgca
    13561 agaacatcga agagctgaat aatgttttga aagaaacagc aacagctatt aaattgtaag
    13621 aaagctttaa ctataacctt aagtttgtaa cctaatgatt tttaataatc aatttgagct
    13681 tttaaataaa gaattggaag cgtcccgacg cttccttttt cgtttgtttt taaggcatga
    13741 taggttctat gttgtcgttc aataagcgca agaaggtcag catttttttc ttagagtatg
    13801 caaaattgct tttctaccca atgatttaaa acggagaggc acccttggat atggtagatg
    13861 gtgaagtaac gcatttcttc tgtgatcacg gtttttttgt cttcttgtag ccagattttt
    13921 aaataatttt cttcgatcag attatttaat ctcttttgaa attggatatc tccgtggttg
    13981 atgacgaata acttgtatac gaacgtattc gtatagacat agtcgatcaa ttgagcgtat
    14041 agatcctcgt agctatgtgt cgtatcgtag gaaatgattt catccaagtc ttggaaaaag
    14101 ctattttcca gtttttcata aagatcatag atatccgtat aatgtgtata aaatgtcgaa
    14161 cggtgaagat cagcagtttc ggtcagttca cggatggtga tgttttgaat ttttttatct
    14221 actaataatt ttgataatgc ttctgctaac gctttttctg tttttttgac gcggcgatcc
    14281 tcttttttca ttttgcagcc tcctaatcaa cataatcaag gaattgtgtc ggttatccga
    14341 caatttcacc gctatctgaa catagataac cgacaccttg tctattataa tataagtata
    14401 gcatatcaat atttaaagaa taggagaata agtgatggaa ttagccttta agcaactgga
    14461 aaagaaagac tacaaaaaag ccattcaatt cgcgattaaa ggtatgcact ttgattggta
    14521 taccaagaac aaactccttt tgaatcttta tggaagatac ttttggtatc tagaattgaa
    14581 tcgcgcgaca caaatcattg ctttatatgc ggatgatgag ttggcaggtg ttttattagc
    14641 agacatcaaa ggaaaatcaa aaaagcatca ttcatttagt caaaagttat atgttaaact
    14701 atttgatttc ttacaaaatg catttgtgaa agacagcaaa ggcgggtatg acgacaccaa
    14761 tgacgaattg ctttcagact atttaaaaaa acattcccca gatggagaaa ttgtcttttt
    14821 agctgccaac cctgatttaa agatcaaggg gatcggtagt aaattattaa aagaatttga
    14881 acgccgagag caaggaaaag aagtatttct gttcacggat agcggctgta cgtatcaatt
    14941 ttatgaacga cgcggtttcg aacggaaaaa agaaaaagat aacgtcatga agatccttaa
    15001 taaagaagta gatctccgct gtatgttata tagtaagatc attcagtaat gttttttact
    15061 atcagaaagc agaagtacca cgaaggtcaa tggaaaaagc gagtaattga tccatttaga
    15121 tgaagatagg tgtagtctca aagatattag ctggcaaaga tatccccagt taaaaataga
    15181 tttctgtatc caacaataaa aatatttttg tatttattag tatatatact ttacaaaaat
    15241 ttgtatatat actaattttt ttgtaggatc aatgaaatga ggggaaaata tgccaaaata
    15301 taaagaaata gccttggctc taaaagagag gatcgttgag ggaaaatatc aggaaggttc
    15361 taaattacct gagcaagaaa gtctggctag agagtttaaa acaagccgtg ttactatccg
    15421 caaagctata caactcctta ttgatgaagg tttgttatat acgagacgtg gatctggtac
    15481 ttttatacga agcaatatca gacaggacaa tgagaatgtc actcaagtaa ataatgtatt
    15541 tggcacttcc tcacaagaaa atacaaatgt gacaagtaaa ataatccgtt ttgatgtccg
    15601 gtttccgaca gattatgaaa ggcggctttc tgcaacctac ttccgatccc cttcctgcaa
    15661 atcatgggta taaaaaatag gcatattaag cgggctagga ggagaaatat tcacatatca
    15721 ttttttcatt ccgcaagctg cgtcaagtta tttggaattt gtagaacaga tgagagaggt
    15781 agaagcagca ctacaaacta cttttcaata agtaggaatt atagaaattt tactcaaaaa
    15841 atcttcatta aaaaatctga ctaaataagt tgagaaaaca gaatatactt caatatccag
    15901 tgatttataa atatcattca ctcatgatac ccagctccgt atcataagtg atgcggtttt
    15961 tatcattgat cctaaaaatc ttagcaagac aaaaagtacg taagaaagta aagactaaat
    16021 acttcggagg aacaaatgaa taacaatgat aggttattgc gattgcgtta tgcgattgat
    16081 ttgaaagata cggatttgat taaagctttt gagctaggcg gtgtaccact gactaaagaa
    16141 gaagctcagg cggtactgac caaagtacag gataagaaca aagataatgc agaaaataat
    16201 gtgtatgaaa aaaccatcaa caaccaagtt tttgacgcat tcctaaatgg cctgattctc
    16261 ttggcgcgtg gtccgcaaaa agataagccg atagtagaca cgccgcaaaa aagcaaggaa
    16321 atcaaatata tcaacaatgt gttgctaaaa aaattaaaaa tcgccttgtc tatgaccact
    16381 gatgatattt tagaagtttt tgctgaggca gaactctatc catcaaaagg cgaaatcggg
    16441 gcatttttga gaaaagaagg ccaacgaaat tttaagcctt gcggagataa atatatgcgt
    16501 aattttctca aggggttagg aatttataat cggagaaaag tataaagaaa tttcatgaga
    16561 aatcaaaaca atggttgttt tgatgagagc tagtttttgt atgagattaa aatattatct
    16621 taatcgagga gctggattgg ttttatgaat gatgaatata agaatgatga agataagatg
    16681 ctttttgaag agatagagaa tcgatgtagg ctcaattttg agcttcgggg aaagatgtct
    16741 ttaatacaac aaaaaaagta tttagcgaat aaatcagagt ttactctggg acatgttgaa
    16801 aaattaatct cggattggat tagtagtaga agcgaattta caaaaataaa acagccgatt
    16861 aaatttgaca tgaaaaaact tttgttaaat aagagtgaga taggtaatag agatcaatat
    16921 attagagcta aagggcaaga aataattgat tcattaggtg aagtgaggtc atacaattat
    16981 ttgtatgtaa cacatagagc tgatggaatg gtcattactg tggggaaatc ttcttctaat
    17041 gatatttttt tagatggaga tcttttttat caactcaata caaatcactt gtctggcact
    17101 gaaaatataa ttttaaggac ggaatatggc aatgaaatat ttgcaaaata tgatgagatt
    17161 ttaaaaaatt atttggactg ggcttggata attccggttg agtcaggaga tgctaagaaa
    17221 ctggagagat tattgggcga tgaactaatt aataaaaaag taccaatttt aaattattat
    17281 tcccatagac aataaaaggc tatttttcat gataatacta gttataactg taaaaatgga
    17341 agaaaatctg ggaaagaagt ttttaaataa gtgtattgtt cgattttaat aaataataag
    17401 caataccatc cgtctgtgtt gcgcaacatc gtactacaat gaatattttt tcacaaaaag
    17461 aaaatcatcc atcgaatgaa tggaaggttt tagtatacac gaggttaatg tcccctcttt
    17521 tataatcaga ggagtgaggt tttttctatg aatgtttcta ttttcttatt gctagtctag
    17581 tttctcttac gatgaaattg ggattctatt ccagttttta ttgatttgct gataaaataa
    17641 cttgaatggt ggatataaac atgataaata agagaagaat aacaaaacaa ggttgtgaaa
    17701 tgattctcaa ataagtataa tagaaagaaa aaggaggaag aatcaatgaa aatagcaatt
    17761 gcaggcgctg gcgcaatggg aagtcgcttt gggttaatgc ttcaccaaag tgggaacgaa
    17821 gtattattaa tcgatggctg ggcagaacat gtccaacaaa tcaaagaaca tggattacaa
    17881 gcaaatttta atggtaaaga ggtagaagca aaactaccaa tcgttcttca atccgaagta
    17941 gaaaaagaag atcaagttga tctgattatt ctatttacca aagcgatgca gctggaaaaa
    18001 atgctgcagg atatccaatc attaatcaaa aaagatacag aggtcttatg tctattaaat
    18061 ggtatcgggc atgaagatat tattgagaaa tttgtaccaa tggaaaatat ctatattgga
    18121 aataccatgt ggacggcagg tcttgaaggt cctggtcagg tcaaattatt tggaagtggt
    18181 tcagtagaat tacaaaattt aggtgatgga aaagaagcag ctgcaaaaaa attagcagac
    18241 aaattgtctg aatcaggatt aaatgctcat ttttctgaca atattcacta ttctatttat
    18301 cgcaaagcat gtgtgaacgg aacaatgaac ggactatgca caatcttaga cgttaatatg
    18361 gctgagctag gaaaaacatc gactgctcat aaaatggtgg cgacgattgt caatgagttt
    18421 gccaaagtag cagcagtaga gaagattgaa ctagatgtcc cagaagtcat tgcacattgt
    18481 gaatcttgtt ttgacccgga aacaattggg ttacattatc cttcaatgta tcaagacttg
    18541 attaagaacc atcgattaac agaaatcgat tatatcaatg gcgcaatttc tagaaaaggg
    18601 aagaaatatg gtgttgcgac gccttattgt gatttcttga ctgagcttgt ccatgcaaaa
    18661 gaagatagtt tgaacgtaaa ataaaggaga gattgaatgg aaacttcaca aaaaatatca
    18721 ccgaaaattt ttctgaataa agtcctcgct ggtacagcaa caggaatcat tattggattg
    18781 attcctaatg cagtattagg tgctatttta aaatatttta gtgcgtatcc ctttgcggta
    18841 acgatcgcac agttagcagt tatttttcaa ttagcaacac ctttgatcat tggtggtttg
    18901 attgccttac aatttggatt tgatccgatg agaatgatgg ttgtagccgg agcttctttt
    18961 gttggttctg gtgtagtcaa attcacacct gatttgggtg aaacgggtgt ttacgttggt
    19021 gctggtacag gcgatattat taatacgatg ctgacagcat cgattgctgt cgggctgatt
    19081 ttactgattg gaaatagttt tgggtcagtg tcgattgtgt taacaccgat tgttgtaggt
    19141 gtaggtgcag gtttacttgg tttttattta ttcccttttg taacagcaat tacttctgca
    19201 attggatcat tgattaataa cttcactacc ttacaaccgc tgttaatgtc tatcttgatt
    19261 ggttgttcct ttgccttttt gattatctct cctatttcaa cagtagcaat cggaatggca
    19321 atccaactag atggtgtttc tgctggtgcg gctgcgatgg gcgtagctgc aaccactttt
    19381 gttttagtag tgaactcttg gaaggttaat aagtcaggtg tgacaattgc gattgcttta
    19441 ggcgctatga agatgatgat gcctaatcta tttagaaaac ctgtgattct tattccttgt
    19501 ttagttagtg cagtgattac agctattcct gtcgctctat tttctatttc aggtacgcct
    19561 gcttctgctg gtttcggtgt ggtcggactt gttggtccgt tggcttcttt agatgcaggg
    19621 ctaaatggct ttcttgttgt tctttgttgg ttagtcatcc ctgtttttgc ggcactaatt
    19681 actcaatttt tatgcgagaa agtatttaaa ctgtatgatc gagaagaagt cttcaaattt
    19741 ttaggctagt agattttact aagaaatttt caatcaatgg atgcgtaagt gagatgtcaa
    19801 caacatctga cttacgtttt tttgccaaaa tatggttttt attttttatt attaggtgtg
    19861 attagtgatt tatcgacacc gtatatcctt ggcctttttt atcccaaact gaatcaaatg
    19921 actaccgtaa ttagtgtgtt tggtgacgta gatagtccag tcagacgtgc tttcttggtc
    19981 tggtcagttg tatctggact tttttttgtt ctttcgttgc ctgcgttgta tcacctattt
    20041 gttggaacat caaaaacact tgcaatactt gctgttgcaa cagttggttt atatgggata
    20101 ggtgattgta tttttactgg gttattcagt atcaatacga atgaaagctc ttggaatcta
    20161 tcgacttgga tccataatac aggttctggg ctaggctatg caggctttct acttttccca
    20221 ttgttacttg ttctgcttta tcgtcaaa
//
LOCUS       DACEDD010000051        19889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEDD010000051 DACEDD010000000
VERSION     DACEDD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.051"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            170..364
                     /locus_tag="IWO45_002116"
     CDS             170..364
                     /locus_tag="IWO45_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333585.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            633..932
                     /locus_tag="IWO45_002117"
     CDS             633..932
                     /locus_tag="IWO45_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333586.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1029..1463)
                     /locus_tag="IWO45_002118"
     CDS             complement(1029..1463)
                     /locus_tag="IWO45_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0333587.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1695..2405)
                     /locus_tag="IWO45_002119"
     CDS             complement(1695..2405)
                     /locus_tag="IWO45_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0333588.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2395..3255)
                     /locus_tag="IWO45_002120"
     CDS             complement(2395..3255)
                     /locus_tag="IWO45_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0333589.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3260..3898)
                     /locus_tag="IWO45_002121"
     CDS             complement(3260..3898)
                     /locus_tag="IWO45_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0333590.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3913..4212)
                     /locus_tag="IWO45_002122"
     CDS             complement(3913..4212)
                     /locus_tag="IWO45_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0333591.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4255..5739)
                     /locus_tag="IWO45_002123"
     CDS             complement(4255..5739)
                     /locus_tag="IWO45_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0333592.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5759..6220)
                     /locus_tag="IWO45_002124"
     CDS             complement(5759..6220)
                     /locus_tag="IWO45_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0333593.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="IWO45_002125"
     CDS             complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="IWO45_002125"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0333594.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7523..8293
                     /locus_tag="IWO45_002126"
     CDS             7523..8293
                     /locus_tag="IWO45_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0333595.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8566..9393)
                     /locus_tag="IWO45_002127"
     CDS             complement(8566..9393)
                     /locus_tag="IWO45_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333596.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9961..11196
                     /locus_tag="IWO45_002128"
     CDS             9961..11196
                     /locus_tag="IWO45_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0333597.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12195..12455)
                     /locus_tag="IWO45_002129"
     CDS             complement(12195..12455)
                     /locus_tag="IWO45_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0333598.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12482..13783)
                     /locus_tag="IWO45_002130"
     CDS             complement(12482..13783)
                     /locus_tag="IWO45_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0333599.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14075..14533)
                     /locus_tag="IWO45_002131"
     CDS             complement(14075..14533)
                     /locus_tag="IWO45_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0333600.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14546..15871)
                     /locus_tag="IWO45_002132"
     CDS             complement(14546..15871)
                     /locus_tag="IWO45_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0333601.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15888..17300)
                     /locus_tag="IWO45_002133"
     CDS             complement(15888..17300)
                     /locus_tag="IWO45_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0333602.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17317..18081)
                     /locus_tag="IWO45_002134"
     CDS             complement(17317..18081)
                     /locus_tag="IWO45_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0333603.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18185..19060)
                     /locus_tag="IWO45_002135"
     CDS             complement(18185..19060)
                     /locus_tag="IWO45_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0333604.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19315..19399)
                     /locus_tag="IWO45_002136"
     tRNA            complement(19315..19399)
                     /locus_tag="IWO45_002136"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19363..19365),aa:Leu,seq:caa)
     gene            complement(19417..19488)
                     /locus_tag="IWO45_002137"
     tRNA            complement(19417..19488)
                     /locus_tag="IWO45_002137"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19454..19456),aa:Gln,seq:ttg)
     gene            complement(19498..19570)
                     /locus_tag="IWO45_002138"
     tRNA            complement(19498..19570)
                     /locus_tag="IWO45_002138"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19535..19537),aa:His,seq:gtg)
     gene            complement(19632..19716)
                     /locus_tag="IWO45_002139"
     tRNA            complement(19632..19716)
                     /locus_tag="IWO45_002139"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19680..19682),aa:Leu,seq:caa)
     gene            complement(19737..19807)
                     /locus_tag="IWO45_002140"
     tRNA            complement(19737..19807)
                     /locus_tag="IWO45_002140"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19773..19775),aa:Cys,seq:gca)
BASE COUNT         6318 a         4104 c         3097 g         6370 t
ORIGIN      
        1 tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc gagtgaaaaa
       61 aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat cattttcact
      121 tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt
      181 agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata
      241 tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat
      301 aaagaacgag ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa
      361 gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga
      421 ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat
      481 accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga
      541 tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt
      601 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
      661 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
      721 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
      781 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
      841 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
      901 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
      961 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
     1021 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
     1081 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
     1141 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
     1201 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
     1261 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
     1321 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
     1381 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
     1441 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
     1501 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
     1561 tccttaaaat tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga
     1621 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     1681 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     1741 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     1801 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     1861 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     1921 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     1981 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     2041 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     2101 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     2161 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     2221 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     2281 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     2341 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     2401 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     2461 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     2521 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     2581 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     2641 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     2701 caaattgcct aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc
     2761 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     2821 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     2881 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     2941 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     3001 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     3061 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     3121 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     3181 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat
     3241 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     3301 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     3361 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     3421 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     3481 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     3541 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     3601 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     3661 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     3721 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     3781 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     3841 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     3901 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     3961 ttctttgact gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg
     4021 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     4081 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     4141 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     4201 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     4261 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt
     4321 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     4381 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     4441 cccgatataa ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     4501 tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt
     4561 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     4621 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     4681 aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     4741 aattcctgga acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag
     4801 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     4861 agaagtcgtc ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat
     4921 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     4981 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat
     5041 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     5101 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     5161 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc
     5221 taaaatcagt cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag
     5281 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     5341 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     5401 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     5461 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     5521 tccaaccaag attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     5581 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     5641 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     5701 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     5761 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     5821 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     5881 ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt
     5941 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     6001 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     6061 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     6121 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     6181 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     6241 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     6301 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     6361 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     6421 ctcttttgga tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     6481 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     6541 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     6601 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     6661 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     6721 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     6781 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     6841 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     6901 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     6961 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     7021 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     7081 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     7141 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     7201 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     7261 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     7321 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt
     7381 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     7441 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     7501 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     7561 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     7621 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     7681 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     7741 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     7801 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     7861 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     7921 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     7981 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     8041 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     8101 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     8161 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     8221 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     8281 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     8341 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     8401 tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     8461 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     8521 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     8581 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     8641 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     8701 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     8761 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     8821 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     8881 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     8941 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     9001 aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa
     9061 aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt
     9121 ataagattct tggtattttg atacagaatt caatgattga atctcattta tttttttcct
     9181 gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca
     9241 aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt
     9301 tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta
     9361 ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta
     9421 tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga
     9481 ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga
     9541 tttctttttg tgttatctga ataattatcg tttacagtta taacacatag tgaaaattgt
     9601 catggcgaga aagtctaaga atatttttaa acataactat ttctttctta cgtgtgtata
     9661 atgacgacag cacacctatc aattaatata attattttat ttttttatgt tctcatgttt
     9721 atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg atcaaaaaat
     9781 tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag
     9841 ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg
     9901 tgttttttag tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc
     9961 atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt
    10021 gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact
    10081 tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg
    10141 aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt
    10201 accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag
    10261 cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag
    10321 attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat
    10381 gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg
    10441 acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa
    10501 aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc
    10561 atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt
    10621 ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag
    10681 cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg
    10741 cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca
    10801 cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa
    10861 tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa
    10921 gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt
    10981 gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa
    11041 ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt
    11101 cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa
    11161 gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta
    11221 atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt
    11281 aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca
    11341 tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct gccatttatt
    11401 cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga
    11461 aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca
    11521 tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca aatgtaggat
    11581 ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc
    11641 cgatgcatac tcctatataa actgccagca atccccacat attgagtaga tcatgtcaga
    11701 atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac tgattgctat
    11761 tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca catcatacat
    11821 attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat
    11881 atttgacttg atcactttgg tagtagatct actaattcta aatccattgc tgataaaatt
    11941 tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa
    12001 aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag
    12061 cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt
    12121 taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgttacaac
    12181 cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa
    12241 ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac attcatatgc
    12301 ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga
    12361 ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa
    12421 aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa
    12481 cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc atctttttat
    12541 cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca
    12601 agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg
    12661 tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca aaataactga
    12721 taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca
    12781 taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata atagaaaaac
    12841 gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga
    12901 tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat
    12961 agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga
    13021 tcagccacat tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc
    13081 acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat
    13141 tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc gcgacgcttc
    13201 gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg
    13261 caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac
    13321 gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca
    13381 aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa acgtttacag
    13441 gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc
    13501 catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca
    13561 tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc
    13621 caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg
    13681 ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg
    13741 gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc
    13801 aataaaatat gaaccattca ttgcatgaca acgcaaagaa tgttcataac taattatata
    13861 attaagaaat tttatttcaa tatattctct taaataatca aatttatttt taagaatcct
    13921 gaaaataagt tttctaaatc ataggagcaa caaaaaaatt cgataattaa caaaaatcaa
    13981 aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa attcacccat
    14041 aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa
    14101 tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa
    14161 tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg
    14221 tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt
    14281 caactgtatc taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta
    14341 tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc
    14401 catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt
    14461 ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt
    14521 taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt
    14581 tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg
    14641 taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct
    14701 ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg
    14761 taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc
    14821 gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat
    14881 tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca
    14941 atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc
    15001 gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat
    15061 caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga
    15121 tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta aaatcggcga
    15181 cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt
    15241 ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc
    15301 aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat
    15361 cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg
    15421 actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga
    15481 caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg
    15541 tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca
    15601 atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca agctttggat
    15661 catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata aatttaacaa
    15721 tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt tcagataatg
    15781 gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac gtactcccgc
    15841 ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta ttcttctaat
    15901 aatttattga attctgccat aacataagtc acttctaagc cgatgataat ttgataattt
    15961 ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg ttcctcgacc
    16021 atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac atccaggata
    16081 ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata atttttactt
    16141 gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc cttagcttca
    16201 cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac gtaatacaag
    16261 gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat tcgaaaattg
    16321 atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa
    16381 cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat aggagaaaga
    16441 aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt tcctgcaccg
    16501 cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt atagatagct
    16561 agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa aaacagtcca
    16621 gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc cttgctagga
    16681 gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg cagtccaaag
    16741 gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc catatcaacg
    16801 agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa gcccaaaaaa
    16861 acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa
    16921 taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca tgccgctact
    16981 aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat aatttctaag
    17041 ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta
    17101 tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt tccaaaaacg
    17161 gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga taaacgcatg
    17221 atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc aagcttctgc
    17281 acatttgatt taacattcat ttttattcct cctctattaa atatcctcat atgttactaa
    17341 ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta aaaattcgac
    17401 ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat ccaaatacgc
    17461 tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta aacttgccaa
    17521 atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc ctacgacatc
    17581 gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa agttgctccg
    17641 aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt catccaaagt
    17701 atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat aaattttttg
    17761 aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg gatgctgata
    17821 ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt tgaggagtgg
    17881 tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt cctttcttag
    17941 tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg tcaaaattcc
    18001 tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa aatcatccga
    18061 attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat aggcgcaacc
    18121 aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata gttacgccct
    18181 tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt tcttgaatgg
    18241 caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa tacgctttgc
    18301 tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct tcctgataaa
    18361 tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc agtgattctt
    18421 tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc gtcatttcta
    18481 ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg atatgcatag
    18541 tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg attcgctcga
    18601 ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct ggcaacataa
    18661 ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata tgatctgtca
    18721 atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc agttcaatca
    18781 cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca ctgaatcgtt
    18841 tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca tcaaatggct
    18901 tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct attccatcgt
    18961 catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt aattcacctc
    19021 ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt gtctccaaac
    19081 aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt gcctaattga
    19141 atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag cgttatcaat
    19201 atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg taattacata
    19261 aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata attataccgg
    19321 cggccggggt cgaaccggca cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg
    19381 ccaattccgc cacgccggca aatttaaaaa taataactgg ggtagctgga ttcgaaccaa
    19441 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaagggc
    19501 gaatgatggg aatcgaaccc acgaatgccg gagccacaat ccggtgcgtt aaccacttcg
    19561 ccacattcgc catcataaaa gatgttattt aatttctcaa taaaaaacta agatgaagac
    19621 atcttagtca ataccggcgg ccggggtcga accggcacgc cctcaacggg cactggattt
    19681 tgagtccagc gcgtctgcca attccgccac gccggcaagt tgatttatta gtactaaggc
    19741 ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc tctgccttac cacttggcta
    19801 taccgccatt atactattat caatctataa aataaagaat aaattataga aactggggta
    19861 gctggattcg aaccaacgca tgagggagt
//
LOCUS       DACEDD010000052        19845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEDD010000052 DACEDD010000000
VERSION     DACEDD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.052"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..935
                     /locus_tag="IWO45_002141"
     CDS             156..935
                     /locus_tag="IWO45_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333605.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            981..1925
                     /locus_tag="IWO45_002142"
     CDS             981..1925
                     /locus_tag="IWO45_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0333606.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1941..2720
                     /locus_tag="IWO45_002143"
     CDS             1941..2720
                     /locus_tag="IWO45_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0333607.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2732..3430
                     /locus_tag="IWO45_002144"
     CDS             2732..3430
                     /locus_tag="IWO45_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0333608.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3458..4222
                     /locus_tag="IWO45_002145"
     CDS             3458..4222
                     /locus_tag="IWO45_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0333609.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            4235..6052
                     /locus_tag="IWO45_002146"
     CDS             4235..6052
                     /locus_tag="IWO45_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0333610.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAVFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6063..6695
                     /locus_tag="IWO45_002147"
     CDS             6063..6695
                     /locus_tag="IWO45_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0333611.1"
                     /translation="MKAIIVGAGGFGKEIAFLLQSVSRYELVGFVDDSLKMQNQELLE
                     KPILGTIDLLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSVFPSVNISGHVILGD
                     KNEIGVGTKIIQNLSIGNTNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            6697..7815
                     /locus_tag="IWO45_002148"
     CDS             6697..7815
                     /locus_tag="IWO45_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0333612.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNEKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHNFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            7808..8425
                     /locus_tag="IWO45_002149"
     CDS             7808..8425
                     /locus_tag="IWO45_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0333613.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKILRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            8433..9647
                     /locus_tag="IWO45_002150"
     CDS             8433..9647
                     /locus_tag="IWO45_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0333614.1"
                     /translation="MPLNILFLTLLDFQSLEESNIYTDLMSEFVKNNHNVSIISPTER
                     KKRIKTHLIEINDKCEILKLRIGNIQKTNLIEKGFSTIQIERKFISGINQYFSNKKFD
                     LVIYSTPPVTFAKVVKFIKKRDNCKSYLLLKDIFPQNAVDLNQLSKNTYIYKYFRKKE
                     VSLYEVSDYIGTMSEQNSQYIRKHNKIDEQILEVNPNSIRINENKIGSPLDKEEIRNL
                     YKIPKEKTIMLYGGNLGKPQGIDFLLNIISSLEKDNKILLLIIGSGTEYGRIRKHINT
                     NNIKNTILIDSLSKNEYKKVESIADIGLVFLDKKFTIPNIPSRILGYMESKKPILAAV
                     DKHTDLNEMIKTGKFGYCSEHGDLNAFLSNMERLKDEKIRNELGKNAFIYLKNNFDVK
                     RSYSIIMNHFEL"
     gene            9663..10685
                     /locus_tag="IWO45_002151"
     CDS             9663..10685
                     /locus_tag="IWO45_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0333615.1"
                     /translation="MFKDKILLITGGTGSFGNAVMKRFLDSDIKEIRIFSRDEKKQDD
                     MRKKYNNDKLKFYIGDVRDINSVKNAMYGVDYVFHAAALKQVPSCEFFPMEAVKTNIM
                     GTDNVVTAAIEMGIQKVICLSTDKAAYPINAMGISKAMMEKVFVAKARTVTDERTMIC
                     GTRYGNVMASRGSVIPLFIDQIKNGKPLTVTDPGMTRFLMSLEEAVELVVFAFQNAKA
                     GDIMVQKSPASTVGDLAQAIKELFHADNEIHIIGTRHGEKRYETLLTKEEYLVAQDMG
                     DFYRVPADTRDLNYDKYFVEGSKELTQEGEYNSDNTQRLSVEQIKERLLELDYVRKEL
                     KEWQNQ"
     gene            10682..11791
                     /locus_tag="IWO45_002152"
     CDS             10682..11791
                     /locus_tag="IWO45_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HAQ0333616.1"
                     /translation="MNILVTGSNGFIGKNLVAELKNEGFTDILSFDRDTPVHLLDEYC
                     EKADFVFHLAGVNRPKDDAEFMTGNFGFTSELLEKLKEHGNTCPVMISSSIQAELNNS
                     YGKSKKAGEDLLKKYQKETGAPVYIYRFSNLYGKWSQPNYNTVVATFCYKIARDEEVT
                     VNDPSAIIELCYIDDVVKELLHCLNGQPYTENGYCAVRETDEVTVGELKELITSFKNS
                     RTNLSVPNLARRVEKNLYSTYLSFLPEDEFSYFLKMNVDNRGSFTEFLRTKDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGEGVIRFRKVDSNEIIEYFVSGEKLEVVDIPVG
                     YTHNIENLGSTDMVTVMWVNEPFDPEHPDTYYVEV"
     gene            11795..12919
                     /gene="wecB"
                     /locus_tag="IWO45_002153"
     CDS             11795..12919
                     /gene="wecB"
                     /locus_tag="IWO45_002153"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAQ0333617.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSAVINRLDRSEAIDHILVHTGQNYD
                     YELNEVFFEDFKLKKPDYFLNAATGTAIETVGNILIKIDPILEEVKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNSRKDDIQHSDVLNRLGLKEGQYFVVSAHREENISSETNF
                     LHLVDSLNTIAETYQLPIIISTHPRTMKMIEAKEIKFHELIQTMKPMGFNDYNKLQIN
                     AKAVLSDSGTISEESSILNFKALNIRQAHERPEAMEEASVMMVGLEKERIMQGLEVLE
                     TQQKDTLRHVADYSMPNVSEKVLRIILSYTDYVNRVVWGR"
     gene            12916..13971
                     /locus_tag="IWO45_002154"
     CDS             12916..13971
                     /locus_tag="IWO45_002154"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0333618.1"
                     /translation="MRIVVNDIAASEGGAMSVLRDLHSDIISSGDENEWIFLLGEKFI
                     EESDNIKVLTFPKIKTSWIKRLDFDFISGKKIINKLQPDIYLSLQNTATIGVKAPQYV
                     FLHQPLPYQKEKNFSFIKKKEFKMATYQHVIGKIYSILFKKTKATIIVQTQWMKENVS
                     SILNNKILKVDTKINIDENIINKQINENKRNMKHFFYPASNIPYKNHDFLIESFSKCK
                     DKELRLFLTLSRDEIDIKDDRIILLGKVPREKVFDIYSKSNLIFPSSIETLGLPLLEA
                     QCFMSPILALETEFSKEALSGYPHKYLFKLDNLDNLSHLIEKISTSSIVFNVENVIYK
                     KENRVSILDILKGENIY"
     gene            13968..15182
                     /locus_tag="IWO45_002155"
     CDS             13968..15182
                     /locus_tag="IWO45_002155"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0333619.1"
                     /translation="MKKFVVAHPAQQHSYKIAEALIEKEMLFKYITSVYLKKGSILNK
                     LLNIIPGENKLRAKGRHSDKICDEEVLLINEFSGLLLLILQRVKFLKHLYKKYWDFHN
                     KKFNKKLIKYINSNIDEISGVIVFDQVSFDFFKKKINIPIILDMSAPSVVFMKSQFEK
                     YGISTLKYDALLKKNNFEINSADFFIGASSITERSLLNMGIEKKQIKIFPYGVNIVKE
                     NDKLMNLRTRKKRLKFLFVGRVTLEKGIGVLDSFFEKFGIEDAWTFAGDYSDSKKFYE
                     KYEDKVQFVGHVSKIKMFELYRDNDVLVFPSLADGFGLAVIEALSNGMYVICSDSTGA
                     KDIIMEGKNGDVFLTGDENDLNNKIQYYKKNINKIDRLKIKETVNDLTWERYNKNIIK
                     SLEYFTVGMNNE"
     gene            15694..16290
                     /locus_tag="IWO45_002156"
     CDS             15694..16290
                     /locus_tag="IWO45_002156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0333620.1"
                     /translation="MKRTALITLALIIFIELILNVLNKKRISLRRLAFLIPFSLFIAA
                     LLIKINKIISSLYDNNIFMRFYSGDTIGESRGDILKLLLEKICTNSNIYFILLGQGIN
                     STIYYTGGLTAHNDFLEVLFDFGIFGLLLYIMIYIIFIKKIRIITNVKDKRSLLYTMV
                     ILMLVSVSSHLIFIPSYVSLLIMNYNYLKNKNYFSKEN"
     gene            16290..17384
                     /locus_tag="IWO45_002157"
     CDS             16290..17384
                     /locus_tag="IWO45_002157"
                     /inference="COORDINATES: protein motif:HMM:NF016145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ0333621.1"
                     /translation="MKKIGILTFHRAENYGAVLQTYALQEYISKMSSNYEVEVIDYRS
                     PFIEKYYKLLSFKAFTNIKSLKRIVYVLLYNGTLKSKRKIFDNFIDKFIRVSLSEYDD
                     KTIRGLDDKYDLFICGSDQIWSPYSAGFDPIYFLDFVKKRNKKNAYAASFGVEIVPEE
                     TIKEYSRRLKNFNVISVREEIGKKIIKNLIEEDVPVSLDPTLLLSKTEWDLIIEKKRK
                     ISEKYLLIYLITETREIIELAKKVAREKKLKLVYINDNIYKTKGIYNYNNCSPSDWLN
                     LFYNADYIVTNSFHGIAFSINFEKEFSVQYLPEPAKVNSRINNILDNYSLKWRINYDG
                     NEKINYSKVNKILVEDRVASQQILKDIMEY"
     gene            17394..18377
                     /locus_tag="IWO45_002158"
     CDS             17394..18377
                     /locus_tag="IWO45_002158"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0333622.1"
                     /translation="MKPKVSIIIPAYNAEYTISRALESALKQTYSNKEIIVIDDGSKD
                     RTLAICKEYQLNNHEIFIFSQKNSGVSVARNLGIRKADGEFIQFLDADDAMEPNMTEK
                     LIEVQRNNDSDYVICGLTIIKDNNIVRTPHLKERNINFINFEDYKYIYPLLAAPCNKL
                     FKKELIVLFEKNFSNGEDLLFNTEYLTKIKKISIIEDCLYKVYLDNENSLNKKMSIST
                     VKSLLLINEYEKEIMNKLFDNDNVLEFNVFNKFKIIQTSLQKIVNNEEKDDVIELINN
                     LRSDSQFLTILEKTNMKNMKSNLIKFCFSYCDSQTIYQLFFLISKVRGYFQ"
     gene            18374..19810
                     /locus_tag="IWO45_002159"
     CDS             18374..19810
                     /locus_tag="IWO45_002159"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0333623.1"
                     /translation="MKDRVKNVIYNAVYQVFIIVIPILTMPYVSRKLGAENLGINTYT
                     QSLVLLLGLFINSSILQIGAREIARQDRISRTKTFITLWLLQVLCGLVIIIVYILIIM
                     GSKYYYYYLLQLPFLISYIFDISWFFVGLEKIKNVVFRNMFVKVCTIFLIFLLIKNKN
                     DLSIYIIIISLGSLVGNFIFFFSLKKYLDFDKIVGLDLSNLRPFINSIVILLVPQVAI
                     QLYQYIIKFSIENSTTTTELSYFDQSQKIARIIIQLVSSISIVLMPVIANLDKNKSRN
                     ALNKIIQLSVNYSLSIAVIFTILLVIISPTFVPIFFGPEFKEMIPHMIISSFIIIPIC
                     VGGVFSNQLILGMGLYKYYATPYLIGGSIGILICLIYKDKMSGLIGCIVLVIVESLVC
                     FFRIIFANKIIDIKENLQGTIKEFFLLFLLLAEVYIVNTIFTPSPSALLNLFYMMIII
                     IQYFVLSTIIKTETAKDLSKRLKSFLIK"
BASE COUNT         7402 a         2801 c         3608 g         6034 t
ORIGIN      
        1 aacagtttgt ggtatgatta tatagggaaa acttgtttac aaatatttct gtgaatatat
       61 tagtttaatt atgtttttta aaataaaaag ctgtcatttt tatgtaagtg aattatatct
      121 taatttatct atcatcaata cttaggagtg aatctatgga aaagccattc gtcaagtcat
      181 tgactgctgt ggcggtaggt gcaggtgcta ttgcgatctg tttgtttggt taccatatca
      241 ataatcagcg acaacatcag caacggatca attatgcaga atctgccatc accaatcaaa
      301 aagacacctt gacttcttta tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa
      361 tttttctaaa ccctgaaata acagaagaaa caatcacaag tttgtcaaat aagctatcct
      421 ctgtcaaact ttcagcagat gattacgaca tcaaagaatc agaattgcct aaagaagctg
      481 ttgcaatcca agatgagaag aaagcaatcg tgacacagtt agatagtgca gaatcaaaac
      541 tgaagatcca gacagctgtc aacgatttat ttactaaaaa tgtatcaaac tggcagcaag
      601 ctgtagacga tgtcatcatc aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg
      661 aaaatatgag cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa tatctaggat
      721 tcgctgatac tcagatcgcg caagtcacgc aactagatca gttattcgat acgatgttga
      781 aggatggtca agtgactgcc accgctactt acgatcaata tttgacggtg ctcagtcaaa
      841 tagagcagat ccggaatgaa aaaatacgcg cagcatacgc aacaaaagca caaactgttg
      901 ctcagcaaat gggctatagc aatataagct attgacagtt aaaacgttga ttcaagcaag
      961 gttagcaaac ggaggaaact atggcaagac gaagaagaaa atcaagaggg aaaaaaatat
     1021 ttctgattat tttagcagtt cttatcgtat tagtattagc aattgtagga gtaggagcaa
     1081 aattatatct ggatctctca cattctattg agaaaactta cgaatcagtg gaacgaaccc
     1141 aaggagaacg aacgaaacca gttgatctgg acaaacaaga atctttctct gttctattga
     1201 tggggattga tactggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg
     1261 ttgccacagt cagtcctgaa gacaaacaga caacgatcgt cagcattcct cgagatacgt
     1321 atgtcgacat cgtaggtcgt gacacaaaag acaagatcaa ccacgcttac gcattcggcg
     1381 gagctgccat ggcgatggat accgtacaaa aatatgtaga catcccaatc gatcactatg
     1441 tctctatcaa catgaaaggg ctaaaagaac ttgttgatgc agttggtggt atcgaagtca
     1501 ataatgacct cactttctca caagatggct atgactttac aatcggtaaa attactttga
     1561 acggggatca ggcattatct tattctcgta tgcgttatga agatccaaac ggtgactatg
     1621 gtagacaaga gcgtcaaaga aagatcgtag aaggcgtagc aaaaaaagta ttgagtctaa
     1681 acggtgtcag caactatcaa tccgttttga atgctctaga gtcgaatatg aaaacagaca
     1741 tgagctttga tgaaatgaaa aaaattgctt tagattatcg ggaggcattt ggtacgatca
     1801 agcaagatca gctgcaagga gaaggcttca tgcaagataa tatctcctat caaaaagtca
     1861 gtgatcaaga acttgatcgt gtaaaaaaag aattaaaaga tcaaatgaat ctagagaata
     1921 aataggaaag tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttgaaacat
     1981 tacgcaaaca cttggtaaca atcatcatct caatgtttgc tggcttggcc atctcaggaa
     2041 tcgtgacgtt tttcatcttg acaccaaaat acagttcaca agcacaattg atcgtgacac
     2101 tgccacaatc agacacagca aatgtcaatg atgtcaatac gaacctgcaa atgatcaata
     2161 cgtataaaga tatgatcgtc agtgatttag tactaaatga agtcaaagat cgtttagaaa
     2221 cagaagacaa tgtgaaaatg actgctggac aaatcaaaga tgcgatctcc gtcaatcagt
     2281 cacaaaattc acaaatgttc tcgatccagg caataagcac gaatgcagtg actgctcaac
     2341 agatcgctaa cacaacttca caagttttcc aagaaaacgc caaagacgtg atgaatgtcg
     2401 ataaaatctc gattatttca ggtgcagttg ccaatacgac acctgtttcg ccaaataata
     2461 aattgaatct agtgattggc ttagtgttag gaatgatggt aggcgtaggt ttggcattct
     2521 tattcgaatt acttgatcgg acagtgaaag atgaaaaata tgtgacggat acattaggat
     2581 tcccaatctt aggaacagtt ccagaaatgt cagcgaaaga attgaacgct ggtatccgta
     2641 aaaaaggaac agctcctgca cgaccaaaag ctacaaacga taaaacagcc ccatcacgca
     2701 gaagccgctc aagagtctag gaggaagaaa aatggcacga acacagaaac aaaaagcagc
     2761 acaaactcat gcagtcagtt tgatcacatt aacaaatcca tcttcaccaa tcgccgaaca
     2821 atatcgtacg atccggacga atatccagtt tgcctccgca gctggtcagc aaatcaaaac
     2881 gattgtcgtg acttcatcag gtcctggaga agggaaatcc accactgcag caaatattgc
     2941 agtcgttttt gctaaatcag gtcaacgagt tctactagtc gatgcagatt tacgtaaacc
     3001 cgtcgtctat aagacattcc agctgaacaa tgccagtgga ttaagtacag cacttagttc
     3061 atcaggaagt gtagcagatg tgatccaacg aacgccagtc gagaacttgt caattttgcc
     3121 tagtggacct aagccaccca acccatcaga attgttaagc tcgccaagaa tggatcaggt
     3181 tctagccgaa gcaagacagt tatttgacgt ggtgatattc gatatgccac cagtagtagc
     3241 cgtaacagat gcacagatca tgtcttcaaa aacggatggc actttattgg tcgtacgtga
     3301 aaatatgtca cgtaaagaat ccttgactaa agcaaaagaa ctgctggaga tggtacaagc
     3361 acgagtctta ggtgttgtgt acaacggagc agaacatagc aaagatgctg gttattacta
     3421 cggaaattaa ggaataagaa gagaggagac aagagaaatg atcgatctac attgccatat
     3481 cctaccagga atcgatgacg gggcagaaaa cttagaagca agtattgcaa tggctgaaaa
     3541 agcgattcag caaggcatca cgcacatttt atgcacacca caccacaata atggaaaata
     3601 cagcaatgaa aaatcacaag tcatttctct agtcgcttcg ttacaagccg aattagagaa
     3661 aagacagttg cctttgacgt tattggaagg acaagaagta cgtattaccg gaacgctcat
     3721 cgaagacatc caccgagatg aaattttatt tacagattta gatgatacat atcttctgat
     3781 cgaatttccg acactggaag tgccgttata tgcagaacgg cttttttttg agttgttaga
     3841 actaggaaag acaccagtga tcgtccatcc agaacgtaac gcgcattttc gcgaagaccc
     3901 aaatcatttg cttccgttcc tagatatggg atgtttggcc caactcactg cgccaagtta
     3961 tgttggggta tttggcaaag acatccagaa aacagccaaa acgatggtca aacataatct
     4021 tgtgcagatg gctgcttctg atgcccatgg ggtctctaag cgcacctttt acttaaaaga
     4081 gtgctatgaa cagattgcca aggactttgg taaggaaaaa gtagtacaga tgaaacaagt
     4141 cgcaaaggat ctgatcaacg gggatgaaat cagttatcca atctatgaag aggtaaaaaa
     4201 gaagaagttt ggtttatttt agggggaatg tagggtgttt gtattaacaa gaagaagaaa
     4261 aattgccaca ttggtatttg tggacagctt gttgttgatt gctgcgaata ttgcagcagc
     4321 taaattcatg aagccgtttg tagctatacc aatggacctt attttaatct ctatcggttt
     4381 gtcgattggt ttttatttat tctatggtag tttattcaaa gtattcactc ggatcaatcg
     4441 ctatacaaat ttaagagaaa tcattgcggt ttttggctca ctatctgcaa gtgctgtctc
     4501 aagtatcatc atattgctgt tcataaatcg gcgatacagt ttacgtttag tcatttttgc
     4561 ttacctgctt tcgctattat tgattattgg tagccgattg atctggcgta tctatgtcga
     4621 aacaaaaaat atgagatatc tatccacaga ttctgctaaa aatacattga ttgtcggtgc
     4681 tggcgaaggc gggcgtattt tatacaacag ctttcttgga tccaaaacgg ctcaagatat
     4741 ccacgttgtc gggtttgtag atgatgatcc aaataaaaga aatacgtatc tttcaggaaa
     4801 aaaagtatta ggtgcattaa aagatattcc ggaattgatt gaaaaatatg acattcaaat
     4861 ggtaacgatc gctatccctt ccttatcaag aaaaaaatta cgaagaatct tcgagttagt
     4921 cgaatccgct cgtgtgaaag tcaacacgat gccttctata gaagaacttg cttcaggaaa
     4981 aattagcgta tcaaaattaa aaacaattga cgtagtggat ttactgggaa gagatgaggt
     5041 aaagctggat attgaatcca tcaaagatca gatcacagac aaagtcattc ttgtcaccgg
     5101 cgcaggagga tcgatcggtt cagaaatctg tcggcaaatc attcaattca atcctgcaaa
     5161 gctattgctt ttaggacatg gggagaactc gatctacttg attgaccgtg agttacgcac
     5221 gcatcatcaa aattgtccta cagaaatcgt accgatcatc gcagatatcc aagatcgcga
     5281 gaaaatcaat gagatcatgg aacaatatca tccagatatc gtctaccatg cagctgctca
     5341 taaacatgta ccactgatgg aatataatcc aaaagaagca gtcaaaaaca atattttcgg
     5401 tacaaaaaat gtagcagaag cagccaaagc agcaagagtg aaaaacttcg tcatggtatc
     5461 gacagataaa gctaacaatc ctccaaacgt catgggttca acaaaacgaa tcgctgaaat
     5521 gatcgtgact ggattgaatg aagaaggctg tacgaaattc tcagctgtca gatttgggaa
     5581 tgtccttggt tctcgcggta gtgtgattcc agtattcaga gaacaaattg ctcaaggtgg
     5641 tccaatcact gtaacagact ttcgtatgac ccgatacttc atgacgattc ctgaagcgag
     5701 tcgtttagtt attcaatctg gcgcattagc aaaaggtggc gaaattttta ttctagatat
     5761 gagtgaacca gtgaagattg tagacctagc caaaaacatg atccgattga gcgggtatag
     5821 cgaagacgaa atcgaaatca ttgaaacagg tatccgtcca ggggaaaaac tatatgaaga
     5881 actattgttg gataaggaaa gaaatgatga agcagtttat gagaaaatct ttgtaggaaa
     5941 catcaaaggt tactcaatac aaaaagtgat ggattttgtc aagagcctgc cgcaagacga
     6001 tgaacaactg gctaaagaca ttgtcacttt tgcaaatgct tcaaataagt agggggagta
     6061 aagtgaaagc aattattgta ggagctggtg gatttggcaa agagattgct tttctattac
     6121 aaagtgtctc tagatatgaa ttggttggtt ttgtcgatga ttcgttaaaa atgcaaaatc
     6181 aagaattatt ggaaaaacct atattaggaa caatagactt gttaatagaa ttagaagaag
     6241 aaactgctat ttttctggga attgcttcgc cagatatcaa agagaaaata tatcagaaga
     6301 taaataaaaa taataaattg attttcccta atttagttgc accaagtgca ctagtgggca
     6361 taaatgttca gttaggaatt ggcaatattt tgatgcccta tacgacttac actgctgacg
     6421 tagttttagg aaatttcaat atgatcaata tcggctctac tatcggacat gatactcaaa
     6481 tcggtaatta taattcagtt tttccaagtg taaatatttc aggacatgtt atattaggag
     6541 ataaaaatga aattggagta ggtactaaaa taatacaaaa tctaagtatt ggaaatacga
     6601 atattatagg tgcaggaagt gtagtaataa aaaatattaa taacaatact aaaaatgttg
     6661 gcgtacctac caaagtaata gaaagatggg attaagttgg aaaataaacg aatattatta
     6721 gcatctcctc atatgagtga ggaaggatat gaacaagagt atgtaaaaga agcatttgat
     6781 acaaattggg tggctccttt aggtgcgaat gtgaatggat ttgagaaaga attagcagaa
     6841 tacgtgggag ctagtcatgc tgccgcatta tcttctggaa cagcagctat acatttagct
     6901 ttgaaagcgg ctggggtcgg taaagatgac atagtgattt gtcagtcctt gacgttctca
     6961 gctactgcta atccaattct ctatcaggga gctatacctg tatttgttga tagtgattac
     7021 gaaacttgga acatgaaacc tgaacatttg cgaacagcgc tagaaaaata tcctaacaca
     7081 aaggctgtat tagtagtaca tttgtacggt ttatcagcta acttagacga aattactaaa
     7141 atatgcaaag aatatgatgt tactctaatt gaagatgctg cagaatcatt aggaacaaca
     7201 tataaaggaa aatatactgg tacattcggc gactatggaa tttattcttt taatggaaat
     7261 aagataatca caacttcagg tggcgggatg cttgtgtcta atgaaaaaga aaaggtggaa
     7321 aaagtgagat tttgggctac acaatctcgt gagaaggcac gccattatca acataatgaa
     7381 attggctata attatcgaat gagcaatatc gttgcaggta tcggtcgagg acaattgaaa
     7441 gttttaaatg aaagaattga gaaaaaacga tatattcata atttttataa aaaagaattg
     7501 tctaaattgt caaatataca attcatgcca gaaaataatt ggaatgaacc gaactattgg
     7561 ttaagcgtca taacaataaa ggggataaat acgaatcatc ttattgatga gttagataaa
     7621 aataacatag aagcccgccc agtatggaaa cctatgcatt tacaacctgt tttcaaaaat
     7681 tatgattata ttggcgatga tgtagcagaa actttatttg acttaggagt atgtttacca
     7741 tcagatacaa agatgacaga tgaagattta aaatatgtag tatctacgat taaaagagtg
     7801 atcaatgatg cgtaaaaaaa ctttctatca gctttttggg aaaagaattt tagatatcct
     7861 attgtcaggc attgctctca tcgttttatc gccgataatc ttaattgtag gttttttagt
     7921 aagaattaaa cttggttctc caattatctt caaacaagaa cgaccaggaa aaagtgaaaa
     7981 aattttttcc atgtataaat ttcgtacaat gactgacgaa agagaccaca atggagaata
     8041 tttacctgat gaaattcgac taactaaatt tgggaaaata cttagagcaa caagtctaga
     8101 tgagctacca gaactttgga atattttgaa gggtgatatg tcgatcgtag gacctcgtcc
     8161 attattagta gagtatttgc cactatatag tgagaagcaa cggaaaagac acaacgtaag
     8221 accaggatta actggactag ctcaggtcaa tggaagaaat gcgattagtt gggaagaaaa
     8281 gtttgattta gatgtttatt atgttgataa aattagtttc tttaatgatc ttattattat
     8341 aattcagaca tgtaaaaaag taatcaagaa agagaatatt aatacaatca ataacaatat
     8401 accagaaaaa tttaaaggga gttaaatata gtatgccatt aaatatttta tttcttacgc
     8461 ttttagattt tcaaagttta gaagaaagca atatctatac tgatttgatg tctgaatttg
     8521 taaaaaataa tcacaatgtg tccattatat ctccaacaga aagaaaaaaa agaataaaaa
     8581 ctcatttgat cgagataaat gataaatgtg aaatcttaaa attaagaata ggaaatattc
     8641 aaaagactaa tttaattgaa aaaggttttt ctactataca aatagagaga aaatttatat
     8701 caggcattaa tcaatatttt tcaaacaaaa aatttgatct ggttatttat tctactccgc
     8761 cagtaacttt tgctaaagtg gtgaagttta taaaaaaaag ggataattgt aaaagttatc
     8821 ttttactaaa agacatattt ccacaaaatg cagttgattt aaatcaactc tctaaaaata
     8881 cttatatata taagtatttt agaaaaaaag aagtttctct atatgaagta tctgattata
     8941 taggaacaat gtcagaacaa aattctcaat atataagaaa acataataaa attgatgagc
     9001 aaattttaga agtaaatcct aattcaatta gaataaacga aaataaaatt ggatcccctc
     9061 tagacaaaga ggaaataaga aatttatata aaattcctaa agaaaaaact ataatgttat
     9121 atggaggaaa cctagggaaa cctcaaggta tcgactttct tttaaatatt ataagtagcc
     9181 tagaaaaaga taacaaaatt ttattgctga ttattgggtc aggaacagaa tatgggcgta
     9241 taagaaaaca tataaatact aataatataa aaaatacaat tctaattgat agtttatcta
     9301 aaaacgaata taaaaaagtt gaatccattg cggacatagg attggtattt ctagataaaa
     9361 aatttactat ccccaatata ccttctagaa tattaggata tatggagtca aaaaaaccta
     9421 ttttagctgc tgtagacaag catactgatt taaatgaaat gataaagact ggaaaatttg
     9481 ggtattgttc tgaacatgga gatttaaacg catttctaag taatatggag cgattaaagg
     9541 atgaaaaaat aagaaacgaa ttaggtaaaa atgcttttat ttatttgaaa aataattttg
     9601 atgtaaaaag aagctattct ataattatga atcattttga attataaaaa ggagcaattt
     9661 aaatgttcaa agataaaatt ttattaatca caggcggcac gggttctttt gggaacgcag
     9721 tgatgaaacg ctttttagat tcagatatca aagaaatacg tatcttttct cgtgacgaga
     9781 aaaaacaaga cgatatgcgg aaaaaataca ataatgacaa attgaagttt tacattggag
     9841 atgttcggga catcaacagt gtcaaaaatg cgatgtacgg cgttgattat gtcttccatg
     9901 cagcagcact taaacaagta ccttcttgtg agtttttccc tatggaagct gtgaaaacaa
     9961 atatcatggg aacagataat gtcgtaactg cagcaatcga aatggggatt caaaaagtta
    10021 tttgcttgtc cactgataaa gctgcttatc caatcaatgc tatggggatt tctaaggcta
    10081 tgatggaaaa agtatttgtt gctaaagcaa gaaccgttac tgacgaacgc acgatgattt
    10141 gtggcacacg ttatggaaat gtaatggctt ctagaggatc agttatacca ttattcattg
    10201 accaaataaa aaatggtaaa ccattaacag tgacagatcc tggcatgaca agatttttga
    10261 tgagtttgga agaagctgta gagttagttg tttttgcctt ccaaaacgca aaagcagggg
    10321 atatcatggt tcaaaaatca ccagcttcta ctgttggcga cctagcgcaa gccatcaaag
    10381 aattattcca tgcagacaat gagattcata tcatcggaac gcgacatggc gaaaagcgtt
    10441 atgaaacact actgacaaaa gaagaatact tagttgctca agatatgggt gatttctatc
    10501 gtgttcctgc agatacacgt gatttgaatt atgacaagta tttcgttgaa ggaagcaaag
    10561 agttgacaca agaaggagaa tacaattcag ataatacaca acgtttatct gtagaacaaa
    10621 tcaaagaaag acttcttgaa ctggactatg taagaaaaga attgaaagag tggcaaaatc
    10681 aatgaatatt ttagtaactg ggtcaaatgg ttttattgga aaaaatttag ttgctgaatt
    10741 aaaaaatgaa ggattcacag acattcttag ttttgataga gacacgcctg tacatttatt
    10801 ggatgaatat tgcgaaaaag cagactttgt ttttcattta gctggagtga acagaccaaa
    10861 agacgatgct gaattcatga caggaaattt tggttttact tcagaattgc tagaaaaact
    10921 aaaagagcat ggcaatacat gtcctgttat gatttcctct tctatccaag cagaacttaa
    10981 taattcttat ggcaagagca aaaaagccgg agaagatcta ttaaagaaat accaaaaaga
    11041 aacaggagca cctgtatata tttatcgctt ctcaaactta tacggtaagt ggtcgcaacc
    11101 aaattataat acagttgtag ccacattttg ttacaaaatc gcaagggacg aagaagttac
    11161 ggtaaatgat ccgtctgcta tcatagagct ttgttatatc gatgatgtcg tgaaggaact
    11221 actccattgt ctgaacggac aaccttatac agaaaatggg tattgtgctg ttcgtgaaac
    11281 ggatgaagta acggtcggcg aattaaaaga actaattaca tctttcaaaa atagtcgaac
    11341 gaatttgagt gttcctaatc ttgcgagaag agtagaaaaa aatctgtaca gtacgtactt
    11401 aagcttctta ccagaagacg aattttctta ttttctaaaa atgaacgtag acaatcgagg
    11461 atcgtttact gaatttttaa gaacaaaaga ccgcggtcaa gtatctgtca atatttccaa
    11521 accgggaatc actaaaggaa accattggca tcatacaaaa aatgaaaaat tcttagtagt
    11581 aagcggagaa ggtgtaatcc gttttagaaa agtagactca aatgaaataa tcgagtattt
    11641 tgtttcagga gaaaaattag aagtagttga tattccagta ggttatacgc acaatatcga
    11701 gaatctggga tcaacggata tggtgacagt gatgtgggta aatgagccat ttgatccaga
    11761 acatcctgat acttattatg tggaggtcta atcaatgaaa aaattaaaag tgatgacagt
    11821 agtaggaacc cgaccagaaa tcattcgttt gtctgctgtt atcaaccgtt tggatcgatc
    11881 agaagcaatc gatcatattt tagtacacac tggccaaaac tatgactatg agctaaatga
    11941 agtattcttc gaagacttca agctaaagaa acccgattat tttctaaatg cagcaactgg
    12001 tacagcgatt gaaacagttg gaaatatttt gatcaagatc gatccaattt tggaagaagt
    12061 aaaaccagat gcattcttag tattagggga tacaaatagc tgtttatgtg caattgcagc
    12121 caaacgtcgc catattccta tttttcatat ggaggcagga aatcgctgtt tcgaccaacg
    12181 agtaccggaa gaaacaaatc gtaaaattgt tgatcataca gctgatatta atctaaccta
    12241 tagcgatatc gcgagagaat atctcttacg tgaaggatta ccagctgatc gtgtaatcaa
    12301 aacaggaagt ccgatgtttg aagttctcaa ttcaagaaaa gatgacatcc aacattccga
    12361 tgtccttaat cgcttagggt tgaaagaagg acaatatttt gttgtttctg ctcaccgcga
    12421 agaaaatatc agctcagaaa caaatttctt acacttggta gatagcttga atacaatcgc
    12481 tgaaacttac caattgccaa tcatcatcag tacgcatccg cgaacaatga agatgatcga
    12541 agcaaaagaa atcaaattcc atgagctgat ccaaacaatg aaaccaatgg ggttcaatga
    12601 ttacaataaa ttgcaaatca atgcaaaagc tgtactaagc gacagcggaa cgattagcga
    12661 agaatcttcc attttgaatt tcaaagcatt aaacatccgt caagcacatg aacgaccaga
    12721 agcaatggaa gaagcaagtg tcatgatggt aggattagaa aaagaaagaa tcatgcaagg
    12781 actggaagta ttggaaacgc agcaaaaaga tactttgcga cacgtcgcag attattcaat
    12841 gccaaatgtt tcggaaaaag tattgcgtat catcctttcg tatacagact atgtgaatag
    12901 agtggtgtgg ggaagatgag aattgttgta aatgatatcg ctgcttcaga aggtggggca
    12961 atgtcagtat tgagagactt acattctgat ataataagta gtggtgatga aaatgaatgg
    13021 atttttttac taggtgaaaa attcatagaa gaaagtgaca atataaaggt actaactttt
    13081 cctaaaataa aaactagctg gattaaaaga ttggattttg attttattag tggaaaaaaa
    13141 attatcaata aattacagcc tgatatctat ttatcattac aaaatacagc tactattggt
    13201 gtaaaagctc ctcaatatgt ttttttacat caaccactac catatcaaaa ggaaaaaaat
    13261 ttttcgttta taaaaaaaaa agagtttaaa atggctacct atcaacatgt tatcggaaag
    13321 atatattcta tattgtttaa aaaaactaaa gctactatta tagtgcaaac tcaatggatg
    13381 aaggaaaatg tttccagtat attaaacaat aaaatactta aggtagatac aaaaataaat
    13441 attgatgaaa atatcatcaa taaacaaata aatgaaaata aaagaaatat gaaacatttc
    13501 ttttatccag catcaaatat tccgtataaa aaccatgatt ttcttataga gagtttttca
    13561 aagtgtaaag ataaagaatt aagacttttt ttaactttat ctagagatga aattgatatt
    13621 aaagatgata gaattatatt attaggtaag gtacctagag agaaagtttt tgacatatat
    13681 agtaaatcaa atttaatatt tccctcatca attgaaactt taggcttgcc attattagaa
    13741 gcacaatgtt ttatgagtcc aattctagct ttagaaacag aattctctaa agaagcattg
    13801 tccggttacc cccataagta tctatttaaa ctagataatt tggacaattt aagtcattta
    13861 atagagaaaa taagtacaag tagtatagta tttaacgttg agaacgtcat ttataagaaa
    13921 gaaaatagag tttctatctt agatatatta aaaggagaga atatttattg aagaaatttg
    13981 tagtagcaca tccagctcaa caacactctt ataaaatcgc tgaagcatta attgaaaaag
    14041 aaatgttatt caaatatatt acttcagttt atttaaaaaa aggaagtata ttgaataaac
    14101 tattaaatat tattccagga gaaaataagt tacgagcgaa aggtcgtcat agtgataaaa
    14161 tatgtgatga ggaggtatta ttaattaatg aattttcagg gttgctactt cttattttac
    14221 agagggtgaa atttttaaaa catttataca aaaaatattg ggatttccat aataaaaagt
    14281 ttaataaaaa attgattaaa tatataaatt cgaatattga tgagatatcg ggagtaattg
    14341 tttttgatca ggtatccttt gatttcttta aaaaaaagat taacattcca ataatactag
    14401 acatgtctgc acctagtgtt gtatttatga aaagtcaatt tgaaaaatat ggaatatcaa
    14461 cattgaaata tgacgcactc ttaaaaaaga ataatttcga aatcaatagt gcagattttt
    14521 tcattggggc ttcttcaata acagaaagat ccttattaaa tatgggaatt gaaaaaaaac
    14581 aaataaaaat tttcccttat ggagtgaata tagttaagga aaatgataaa ttaatgaatc
    14641 taagaacaag aaaaaaaaga ttaaaatttt tatttgtggg aagagtaacc ttagaaaaag
    14701 gaattggggt tttagatagt ttttttgaaa aatttggaat tgaggatgca tggacatttg
    14761 ctggcgatta ttcagattct aaaaaatttt atgaaaaata tgaagataag gtacaatttg
    14821 tgggacatgt atcaaaaata aaaatgtttg aactgtacag ggataatgat gttttagtat
    14881 ttccttcttt agcggatggt tttggtttag ctgttattga agcattaagc aatggaatgt
    14941 atgttatatg ttcagattct acaggtgcta aagatattat tatggaaggc aaaaatggag
    15001 atgtgtttct tacaggagat gaaaatgatt taaataataa aatacaatat tataagaaaa
    15061 atataaataa gattgatcgt ttaaaaatta aagaaactgt aaatgattta acatgggaac
    15121 gttataacaa aaacattata aaatcattag aatattttac cgtaggtatg aacaatgaat
    15181 aaattattaa tgatgttaaa ctttttttat ctctacgtat acatgattga cgcatacggt
    15241 ataataaata atgatttata tctaagaatg gttttgatta tcttatttat aatgataggt
    15301 ttaataaatt gtataaaaga catcatgaat aaaatataca gtaaagataa tctttttttt
    15361 acgttgtatt taatctattt tctaagtacg caatatatgc tttggaatat tgatatatct
    15421 cagctaataa tgattatcta tactccaatg atgtttatag ctttttctag gtttcatatc
    15481 gacagatact tttatgagaa atttaccttt agttatattt tgcttatgac ttttacttac
    15541 ttttatactc gtttttttct taatataaat aatggtttag tgataaattc ttgttattat
    15601 ttgttattaa ctgttcctat agtttatttg tttgttaatc cttatagaaa aaatattagc
    15661 tttttattaa ttagtatcat aattttattt tcaatgaaaa gaacagcatt gataacatta
    15721 gcactaatta tttttattga gttaatttta aatgttttga ataaaaaaag aattagttta
    15781 agaagattag catttcttat accgtttagc ctttttattg cagcattgct aattaaaata
    15841 aacaaaataa tatctagttt gtatgataat aatatattta tgcgatttta ttctggagat
    15901 acaataggcg aatctagagg tgacatttta aaattattat tagaaaaaat atgtacgaac
    15961 tcaaatatat attttatttt attaggacaa gggatcaact caactatata ttatacaggt
    16021 ggtttaacag cccataacga ctttctggag gttttatttg attttggcat ttttggtcta
    16081 ttattatata ttatgattta tataattttc ataaaaaaaa taaggattat tacaaacgta
    16141 aaagacaagc gatcattgtt atatactatg gtcatcttaa tgctagtttc cgtaagtagt
    16201 catctaatat ttattcctag ttatgtaagt ttattaataa tgaattataa ttatctgaaa
    16261 aataaaaatt acttttcaaa ggagaactaa tgaagaaaat tggtatttta acatttcata
    16321 gagctgaaaa ttatggagct gtattgcaga catatgcttt acaggaatat attagtaaaa
    16381 tgagtagtaa ttacgaagta gaggtaattg attataggtc accatttata gaaaaatact
    16441 ataaattact aagttttaaa gcatttacta atattaaaag tcttaaaaga atagtatacg
    16501 ttttattata taatggtacg ttaaaatcaa aaagaaaaat ttttgataat tttattgata
    16561 aatttattcg tgttagctta tcagagtatg atgataaaac aataagaggc ttggatgata
    16621 aatatgatct ttttatctgt ggaagtgatc aaatttggag tccatactcg gctggtttcg
    16681 atcccatata ttttttagat tttgtaaaaa aaaggaataa aaaaaatgct tatgctgcta
    16741 gttttggagt agaaattgtc ccggaagaaa ctataaaaga gtatagtcga cgtttaaaaa
    16801 actttaatgt aatatcagtc cgtgaagaga ttggaaaaaa aattattaaa aacttgattg
    16861 aagaagatgt acctgtatca cttgatccaa cactattact tagtaaaact gagtgggatt
    16921 taatcattga gaaaaaaaga aaaatttcag agaaatattt attgatatac ttaattacag
    16981 aaacaagaga aattattgag ttagcaaaaa aagtagcaag agagaaaaaa ttaaagttag
    17041 tatatataaa cgataatatc tacaaaacaa agggaatata taattataat aattgttcac
    17101 ctagtgattg gctcaattta ttttataatg cagattatat tgtaacaaat tcatttcatg
    17161 gaattgcttt ttctataaat tttgaaaaag aattttctgt acagtattta cctgaaccag
    17221 cgaaggttaa ttccagaatc aataatattt tagataatta tagtttgaaa tggagaatta
    17281 actatgatgg taatgagaaa attaattata gtaaagtaaa caaaatactt gtagaagata
    17341 gagttgcctc ccaacaaata ctaaaagata ttatggaata ttgaggtggt attatgaagc
    17401 caaaagtatc tataattatt cctgcatata atgctgagta tactatttcg agagctctcg
    17461 aaagtgcact taaacaaact tattctaata aagaaataat agtcattgat gatgggtcta
    17521 aagatagaac attagcaatc tgtaaagagt atcaattaaa taatcatgaa atatttatat
    17581 tctcacaaaa aaattcaggt gtatcagtag ctagaaattt gggaataaga aaagctgatg
    17641 gagaatttat acaattttta gatgctgacg atgctatgga acctaatatg actgagaaac
    17701 ttattgaagt tcagcgcaat aatgattctg actatgtaat ttgtgggtta actattataa
    17761 aagataataa tatagtgaga accccacatc taaaggaaag aaatatcaat tttattaatt
    17821 ttgaagatta taaatatatc tatcctttgc ttgctgcacc atgcaataaa ctttttaaaa
    17881 aggagcttat tgtactattt gaaaaaaatt ttagcaatgg agaagacttg ttgttcaata
    17941 cggaatattt aacaaaaata aaaaaaattt caatcattga agactgttta tataaggtat
    18001 acttagataa tgaaaattct ttaaacaaaa aaatgagtat atctactgta aaatcgttat
    18061 tattaatcaa tgaatatgaa aaagaaataa tgaataaatt atttgataat gataatgttc
    18121 ttgaatttaa tgtttttaat aaatttaaaa tcatacagac gtctttacaa aaaatagtta
    18181 acaatgaaga aaaagatgat gttatagaat tgataaataa tttaaggtca gatagtcaat
    18241 ttctaacgat attagaaaaa acaaacatga aaaacatgaa aagcaattta atcaaatttt
    18301 gttttagtta ttgtgatagc caaacaattt atcagttgtt ttttttaatt agtaaagtac
    18361 gaggttactt tcaatgaaag atagagtaaa aaatgtaatc tataatgcag tatatcaagt
    18421 atttataata gttattccta tacttactat gccatatgta tcaagaaagt taggggcaga
    18481 aaacttaggt ataaatacat atactcaatc cctagtattg cttttaggat tatttatcaa
    18541 ttctagcatt ctacaaattg gcgcaagaga aatcgctaga caggatagaa ttagtagaac
    18601 aaaaacattt attacacttt ggttattaca agtactatgt gggttggtta ttatcatagt
    18661 gtatatatta atcattatgg gtagcaaata ctattattat tatttactac aattaccttt
    18721 tttaattagt tatatatttg atatatcttg gttctttgtc ggattagaaa aaataaaaaa
    18781 tgttgtattt agaaatatgt tcgtaaaggt atgtacaatt tttttgattt ttctattgat
    18841 aaaaaataaa aatgatcttt ctatatacat cattattatt tctttgggat cgttagttgg
    18901 taattttata tttttcttct ccttaaaaaa atatctcgat ttcgacaaga ttgtcggttt
    18961 agatttatct aacttaaggc catttattaa ttcaattgta atattgcttg ttcctcaagt
    19021 ggctattcag ctatatcagt atataataaa atttagtatt gaaaattcta ctacgactac
    19081 tgagttatct tattttgatc aatctcaaaa aattgctaga ataatcattc aattagttag
    19141 ttcaattagt attgtattaa tgccagtcat tgcaaatcta gataaaaata aatccagaaa
    19201 tgctctgaat aaaattattc agttgtcggt aaattattca ttaagtatag cggtgatatt
    19261 tacaattttg cttgtaataa tttcacccac tttcgttcca attttttttg gtcctgaatt
    19321 taaagaaatg atacctcata tgataatctc atcttttatt ataattccaa tttgtgtagg
    19381 tggagtcttt tctaaccagc tcattttagg gatggggttg tataaatatt atgctacacc
    19441 atatcttata ggtggaagta taggaatttt aatttgctta atttataaag acaaaatgag
    19501 tggattaatt ggatgtatag tactagttat agtagaatca cttgtttgct tttttagaat
    19561 aatatttgca aataaaataa tagatataaa agaaaattta caagggacaa taaaggaatt
    19621 cttcttatta ttcttattat tagctgaagt atacattgta aataccatat ttacgccaag
    19681 cccatcagcg ttattaaatt tattttatat gatgatcata attatacaat actttgtttt
    19741 aagtactata ataaaaacag agactgcgaa agatttatca aaacggttaa aatcattttt
    19801 aattaaataa cactttacca aactaaatct ggaatagaag tttat
//
LOCUS       DACEDD010000053        19694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEDD010000053 DACEDD010000000
VERSION     DACEDD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.053"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(216..446)
                     /locus_tag="IWO45_002160"
     CDS             complement(216..446)
                     /locus_tag="IWO45_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333624.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="IWO45_002161"
     CDS             1063..1725
                     /locus_tag="IWO45_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333625.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="IWO45_002162"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="IWO45_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0333626.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="IWO45_002163"
     CDS             5145..5459
                     /locus_tag="IWO45_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0333627.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="IWO45_002164"
     CDS             5472..5846
                     /locus_tag="IWO45_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0333628.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="IWO45_002165"
     CDS             5856..6200
                     /locus_tag="IWO45_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0333629.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="IWO45_002166"
     CDS             6271..7623
                     /locus_tag="IWO45_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0333630.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="IWO45_002167"
     CDS             7683..8825
                     /locus_tag="IWO45_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ0333631.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="IWO45_002168"
     CDS             8850..9104
                     /locus_tag="IWO45_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333632.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="IWO45_002169"
     CDS             9360..10544
                     /locus_tag="IWO45_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0333633.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10559..10678
                     /locus_tag="IWO45_002170"
     CDS             10559..10678
                     /locus_tag="IWO45_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0333634.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            10683..10904
                     /locus_tag="IWO45_002171"
     CDS             10683..10904
                     /locus_tag="IWO45_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333635.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="IWO45_002172"
     CDS             10917..11417
                     /locus_tag="IWO45_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0333636.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="IWO45_002173"
     CDS             11514..13361
                     /locus_tag="IWO45_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ0333637.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="IWO45_002174"
     CDS             13364..14260
                     /locus_tag="IWO45_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0333638.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="IWO45_002175"
     CDS             14310..14699
                     /locus_tag="IWO45_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0333639.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16188
                     /locus_tag="IWO45_002176"
                     /pseudo
     CDS             14686..16188
                     /locus_tag="IWO45_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="IWO45_002177"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="IWO45_002177"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0333640.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18893..>19694
                     /locus_tag="IWO45_002178"
     CDS             18893..>19694
                     /locus_tag="IWO45_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0333641.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DACEDD010000054        19473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEDD010000054 DACEDD010000000
VERSION     DACEDD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.054"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            126..3653
                     /locus_tag="IWO45_002179"
     CDS             126..3653
                     /locus_tag="IWO45_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0333642.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IWO45_002180"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IWO45_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0333643.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IWO45_002181"
     CDS             7441..7782
                     /locus_tag="IWO45_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333644.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IWO45_002182"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IWO45_002182"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0333645.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IWO45_002183"
     CDS             9139..11559
                     /locus_tag="IWO45_002183"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0333646.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IWO45_002184"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IWO45_002184"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0333647.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="IWO45_002185"
     CDS             12993..13730
                     /locus_tag="IWO45_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333648.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="IWO45_002186"
     CDS             13744..14559
                     /locus_tag="IWO45_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0333649.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="IWO45_002187"
     CDS             14572..15219
                     /locus_tag="IWO45_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0333650.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="IWO45_002188"
     CDS             15235..15891
                     /locus_tag="IWO45_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0333651.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="IWO45_002189"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="IWO45_002189"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0333652.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="IWO45_002190"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="IWO45_002190"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0333653.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="IWO45_002191"
     CDS             18233..18751
                     /locus_tag="IWO45_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0333654.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="IWO45_002192"
     CDS             18858..19358
                     /locus_tag="IWO45_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0333655.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6551 a         3343 c         4181 g         5398 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgtgcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc gtttaactcc aagaaataag aag
//
LOCUS       DACEDD010000055        18956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEDD010000055 DACEDD010000000
VERSION     DACEDD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.055"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..563)
                     /locus_tag="IWO45_002193"
     CDS             complement(<1..563)
                     /locus_tag="IWO45_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0333656.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IWO45_002194"
     CDS             complement(750..1400)
                     /locus_tag="IWO45_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333657.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IWO45_002195"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IWO45_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0333658.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IWO45_002196"
     CDS             complement(2912..3910)
                     /locus_tag="IWO45_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0333659.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IWO45_002197"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IWO45_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0333660.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IWO45_002198"
     CDS             complement(4926..5657)
                     /locus_tag="IWO45_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0333661.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IWO45_002199"
     CDS             complement(5661..6926)
                     /locus_tag="IWO45_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0333662.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IWO45_002200"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IWO45_002200"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0333663.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IWO45_002201"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IWO45_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0333664.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IWO45_002202"
     CDS             12268..13770
                     /locus_tag="IWO45_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0333665.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IWO45_002203"
     CDS             13772..14509
                     /locus_tag="IWO45_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333666.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            14515..14673
                     /locus_tag="IWO45_002204"
                     /pseudo
     CDS             14515..14673
                     /locus_tag="IWO45_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(14680..15873)
                     /locus_tag="IWO45_002205"
     CDS             complement(14680..15873)
                     /locus_tag="IWO45_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0333667.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="IWO45_002206"
     CDS             complement(15952..16158)
                     /locus_tag="IWO45_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0333668.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="IWO45_002207"
     CDS             complement(16151..17293)
                     /locus_tag="IWO45_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0333669.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="IWO45_002208"
     CDS             17805..18440
                     /locus_tag="IWO45_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333670.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="IWO45_002209"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="IWO45_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
BASE COUNT         5484 a         3984 c         3312 g         6176 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtat
//
LOCUS       DACEDD010000056        18896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEDD010000056 DACEDD010000000
VERSION     DACEDD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.056"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IWO45_002210"
     CDS             complement(<1..2232)
                     /locus_tag="IWO45_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333671.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IWO45_002211"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IWO45_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            complement(4140..4901)
                     /locus_tag="IWO45_002212"
     CDS             complement(4140..4901)
                     /locus_tag="IWO45_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0333672.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            5001..6722
                     /locus_tag="IWO45_002213"
     CDS             5001..6722
                     /locus_tag="IWO45_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333673.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            6737..8521
                     /locus_tag="IWO45_002214"
     CDS             6737..8521
                     /locus_tag="IWO45_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333674.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8902..10455)
                     /locus_tag="IWO45_002215"
     CDS             complement(8902..10455)
                     /locus_tag="IWO45_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0333675.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10803..13217)
                     /locus_tag="IWO45_002216"
     CDS             complement(10803..13217)
                     /locus_tag="IWO45_002216"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0333676.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            complement(13644..15662)
                     /locus_tag="IWO45_002217"
     CDS             complement(13644..15662)
                     /locus_tag="IWO45_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0333677.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            16032..16688
                     /locus_tag="IWO45_002218"
     CDS             16032..16688
                     /locus_tag="IWO45_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0333678.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            16688..17644
                     /locus_tag="IWO45_002219"
     CDS             16688..17644
                     /locus_tag="IWO45_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0333679.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            17644..18210
                     /locus_tag="IWO45_002220"
     CDS             17644..18210
                     /locus_tag="IWO45_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0333680.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            18646..18876
                     /locus_tag="IWO45_002221"
     CDS             18646..18876
                     /locus_tag="IWO45_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333681.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         5552 a         3786 c         3353 g         6205 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaaacaatc attcttaaaa agaatatata aaaataacaa taacctttgt gatttttctt
     3601 gtttgaagaa tgttagaaga aaaatctcta aatacttata attattttat aagcaaaaaa
     3661 atgctatcgg tagtatcacc gatagctgag gaaagtcaca gtagggacta tttgattaaa
     3721 aaatatgttt attcaatcca tctatttatt tttgccttat taacatattg taataatgta
     3781 agcaacaatg tattttaagt gtaatgcttt tatgcaccta tttaatttat cattctcatc
     3841 tgatacaatc tcactcataa caatatgaat ttttcttctc attagtctca gttcaccatg
     3901 aattatacat aacacccaat acaattattt ttccatactt ttttaagagg acaattacat
     3961 cgtatatatc gctaagaatt gcaaccattc catttttaaa gacatatttc ctctaaaaaa
     4021 ctaaaattct atctatccac gactaataat cccccacatt aaacccgtaa tcgaaccaga
     4081 aaatttttct ttataactaa aacaaccatc attttcgagt tatcttaaac acatatttcc
     4141 tattttttca tttctatcca tgcatgataa tattcagttc tgatattgtt ttcatccgta
     4201 ttcgtcaata gatactggcc gatcacttct cctgttttcc atcctttttc atacattctt
     4261 tctctgattt ctaaaatatt attacgttcc gcatggttca tctcggattt caataagaat
     4321 cgacgataaa cagccgtatc ttttttctcc caaatcacgt cttgagtaaa taaatgtact
     4381 tcactagggt cgataaaaaa tccatacatc gtctgtgaac tggaagctgg catactcatc
     4441 atcccaaagc ttgaatagtt aggtagaaat tttttgattt ccacaatatc ttcaaactcc
     4501 gttgagatca tacagtcaaa agggatctct tctacaggaa attcttcctc gtttgtatcg
     4561 atcatcaatt tcaactgttt tttttgctcc cttatctctt tttgtttttg tttcatcacc
     4621 accagttctt tatggattct tctttctgtt tcatcaagtg tctcatataa ctccgtcaat
     4681 gtttttccat ataaattctt catctgtttg atcggtatat ctaattttct ataaaaaacg
     4741 atatcataaa gcataaaaat atcatcgata tcaaacaatc gataatcatt ctcttcttgt
     4801 ctacttgaac ggattaatcc ttgttcatcc caatagcgga tcgttgattt tggcaaatca
     4861 aatagttccg atacttggcc aatcgtatac atttttttca ttttttctca cctctttttc
     4921 agtttattct tttttctatt gaccttcaag ttacttgaag gtatagaatc gaaaagtatc
     4981 aggaaaaaag gaggaataat atgtctatct taaaaacatt cttacagaaa aacaaaggac
     5041 tcgctctcct tacctttgtg atgatctgtt tagaaatcgc cggtacatta ggtgtaccaa
     5101 aattagtagc aaagctgatc gacacaggta tagcaagtgg cgaagcctca gttataaaaa
     5161 aaattggagt tgaaatgctg cttgtagcgt ttctaggaac aatcgctgca gttgcctcta
     5221 gttacttttc agcattggct tccgctaggt ttggattcca aacgcgggag aaatatttcc
     5281 gtaaattcca acagctgtca atgaaggata tcagtcgttt tagttctgga tctcttttga
     5341 caagaatgac gaatgatgtc gataatgtcc agcaaatgat tgttttattt tgccaaatga
     5401 tcttaccagc accggttatt tgcgttttca ccattctcat gatgtttcgt tattctctat
     5461 tactaacatg ggttacattg atctctgttg ttttttatgt atggattgtt tatcgactga
     5521 tgaaacgagg ggcaccttta tctttgagta tccagccaaa aatggatcgg atcaccatca
     5581 cattgagaga attctttact gggattaata tgatccgtgc ctttgataat caatcatatg
     5641 aagaaaagcg aacgaataag agctttgctg actatgctaa tcaaatgatc aaagtgaacc
     5701 gaatatttgc ttgggtcact ccaattgctt ttttgctcat gggaatcgtt tatgcttcta
     5761 ttttgtggtt tggcggaaat cttgtagcag aaggagcatt acaaatcggt gtcgtgactg
     5821 ctgtagtcga atactctatg ctgacacttg cttacttgat gatcgcagct atggtattgg
     5881 tcattatccc taaatcgttt gcctcattac gacggataga agaaatactg caagcagaga
     5941 tcgaaattga ggaagagacg atcaaaacag tagctgaacc aacctttgat cagacaaatg
     6001 cagctgcttt tgaacatgtc acatttaaat atactgaaat ctctgatcca gttctagaag
     6061 atattgattt cacaatacct aaaggaaaaa caacagcgat cgttggcggg accggatctg
     6121 gaaaaagtag tttggcgaaa cttctcttga gacttagtga ccctacgata ggaaaagtaa
     6181 cccttggtgg tattcctctt actcaaatga ctcaagaaca gatccgctcc cacatcagtt
     6241 atgtccctca gaaggctttc ttattcagcg ggacaatttt aagcaacttg cagatgggaa
     6301 atgaatatgc ctcaacagaa gagttgtcta aagcgatcaa gatcgctcaa ttagaagaac
     6361 tagtggacac ccttcctgac ggattggaca gctttgttgc tcaaggcggt tctaattatt
     6421 cagggggcca gaaacagcgg atatgtattg ctcgagcgtt gatcaaacca gcagagatct
     6481 atgtattcga tgatagtttc tccgctttag actataaaac agatgcagca ttaagagcag
     6541 cccttcataa acacatgtcg gagaaaactc tcgtgattgt tgctcaacgc ctcagtacaa
     6601 tccaacaagc agataacatc attgttttag atgaaggaaa aatcgttgga caaggtactc
     6661 atgaagaact actgtctaat tgccaaactt atcaggagtt tgcaaaatca caaggaatat
     6721 agaaagaagg gatacgatgg aaaagaaaaa aacgttgaac acgcgtgctt tgatacgttt
     6781 ttggaaaatg atcaaacctg aacataaata tttttacgga ttgtttctat gcagtttatt
     6841 cgggaaccta ttagtcgtag ccatgccctt gatcatggga atcgggatcg atgatctctt
     6901 atcagaaata agaaaaacag gtattggaca gctttctttc agtgatatca aacaagcatt
     6961 gttgttccct gtcttattgc tgatcggctg ttcagtctta agcagcatca cttcatttat
     7021 tcaggaacgg acaatggcag cgttaagcga aaagatcaca ttgagggtgc gaaaagaaat
     7081 cacaaaaaaa ttcaaaacat taccgatgtc gttttttgac aatcatcaag taggtgacat
     7141 cattagtcgg acaactaccg gtctcaatca actttctcaa gtgttattga caggtatcaa
     7201 tcaatttttt acttctgtcg taacaatagc tgctgctggt ctgatgctgt tttatatcga
     7261 aagcaagctt actttgctcg tcttattgct gatcgttggc agttcaattg ttacaactaa
     7321 gattgcgaac aaaaacaaac agatttctga acagaatcag tatgaactgg gtgttttaaa
     7381 taatcgaatg gaagaaatct tagctggtaa tctagtggtc aaaacattca atcagcaagc
     7441 tcatgcagaa gctgctatac acgaagtaaa taaaaagcaa tacaacgctt ttaaacgtgc
     7501 tcaatttatg aactgtgcta tctatccggc cattcgtttc attaatcaac tcgccttcat
     7561 cataagtgcg gtattcggtg ctatgcttgt tctatcaggt ggtgtgacga tcggcttctt
     7621 acaagcttat cttcagtaca tcaatcagat ttctgaacca atttctaccg cttcttatgt
     7681 gatcaactcg atccaatccg caatggcagc aatcgatagg atatttgaga tattagatga
     7741 aacagatgaa attccagacc ttccagatgc aagactttta gaacaaccga aaggaaaaat
     7801 agctttcaaa aatgtacagt ttggttacac atcagataaa atattgatga atcaagtcga
     7861 tttttcagta aagccaaaac aaacggtagc gatcgttggt ccaactggtg caggaaaaac
     7921 gactttagtc aatctgctga tgcgttttta tgaactgaat aaaggaaaga tcgaattcga
     7981 cggtcttgat attaccaagc tttcacgtgg agaactacgt cgttcttttg ggatggtttt
     8041 acaaaatacc tggctgtttg aaggaacggt agctgataat atcgcctacg ggaaaatgga
     8101 tgccacaaga gaacaaatta tcgaagcggc tgaaatcgca caatgtgatc actttattcg
     8161 aacactgcca gacggatatg atacgatcat ttctagcgaa aacggcacgt tatctcaagg
     8221 acagcaacaa ttattgacaa ttgctcgagt cattctagcg aatcctcctg tcgttatttt
     8281 agatgaagca acttctagcg tagatacacg aacagaagca ttgattcagc aagcaatgga
     8341 agctgtgaca aaagatagga caagttttgt gattgcacac cgtttgtcaa cgattgaaaa
     8401 tgcagacatg atccttgtaa tgaagaatgg cgatatcgtc gaaaaaggaa cacattctga
     8461 actactccaa tcaccaacgc tttatgccag tctttataat agtcaatttc agatagcgta
     8521 acaaacatta tctaaaaagc tatcgatcat gtatgattag tccatgatcg atagcttttt
     8581 taaatgtact agtggtgcca ctagaaacac aaagaataat actaatgata ctttttggat
     8641 agctccttca gtttcgttcg gtaagtagtg acaaaagact tgtcacaatc cctttttacg
     8701 aataaacggt gttcaagaga caacctatcg gcaataagac acgatcttgt aaaatacgag
     8761 aagctatact tcaaaatcgc ttcttgttgc tcttgtcaac tccgaatcac gagttacact
     8821 attcaacaaa tatgagaagc tgttttttga atttgtttct tgtgactcgg tgctgaacgt
     8881 caggctcaca atctctcttc tttaatctcc ttgttctact tgcgatagtt gctgaacgaa
     8941 ttgccatgtt tccccgttat cagttgaacg gaagaggcag gcaaacattt tccctgcatc
     9001 gattggtcct tttttcaaca aataaaattt tgtctctaac gtattcgcgc ttatttgagt
     9061 aacttcattt ggtgattgaa aaagatctat cccccctgtc tgatagtttt ccggaatcga
     9121 cagaactgat tctttgaatg agcgtcctcc gttgtttgta taaaacaaac tttcacgtgt
     9181 cgaaacaaaa cctaaagaag ttgtaacgaa gctgacattt tgtactggct gattgatcat
     9241 tgtacttcca ctttttatcc aagtgattcc gccatccata gtagtgtaaa tgtacaaagt
     9301 ttctgcagac atcccactgg ctgatgtaca gataaataga ccatcgttgc cataaaatac
     9361 cgcttttcga taacgtatcg gctgaactga ttgggtgata atagacgttt gccatgtttt
     9421 accattatct cttgaagtca gcagacgtac atcatcccca gaagaataga taaaccatga
     9481 gaaatcggcg gaaatatcat atgtgttgtc catccaataa ccttcaggaa tcagccccgt
     9541 tgtcagggtg tattcgccaa aacgcaacca ttgcgaatca atcggcacaa atacccaatt
     9601 agcaccacta tctgttgtca tatagagaga gtgcgtctct gtatcaagtt tgagatcatt
     9661 tatcttttgt gttgtcaaat ttaccttttg cttaggatca gctaacaacc cttcatttgt
     9721 cttttgtata tgcgtatcgt tagttgagtt cattgtcatg tgagaagcag catttatatg
     9781 agaagacatt tttggctgag tgtatctccc ataatagatt gttccagcta aaagaatgag
     9841 gaaaaaacca gtgactaatg tactcatctt agttgtcagg tatccaccag gcgaagactg
     9901 gcttttttcc catctgggtt cgttttctag taaaaaaata tggaatgtgt tcattccgaa
     9961 ttttgatttg ctttcgccta agtagatagg taaatcatca agtgatctca gcaataactg
    10021 gcaatcactt ttttgtatcc gaaaagcttg actcgtcgaa gtcttctcaa aataaagata
    10081 gtcatcatct tgagaagttg gtttattatg taaaaactgt tgtgctcttt gactaagcca
    10141 ttgatcgaaa tgacgcgaca attgatggta acggagccaa ataagtagaa gaaaaacaat
    10201 aagcacaaca attgttattc cgccaactcc ccacgaaaag tgatacaaat tatttaattt
    10261 ttgcatccct tcagctgaac ggactactgc actcatatgg cgatatagga acgatacagc
    10321 tccgaaaaaa agcaaaccag agaaaaatac taagcttaaa agcttgaaca ttattctttt
    10381 cgctcttttt ttcgctttct cgataatttt ttgtttagct gataatactt cgataataaa
    10441 tagttcttga tccatttgtc tctccttttc tcatacttca ttcattatgc gtcaaaatcc
    10501 atacaatcac tagtcccact aataaaaaat ttatgaattt ggtatgtact ctcaatttca
    10561 ggtagtttat acatcaaaaa atactaagat taatagacaa aaatttgatg attacagaga
    10621 tctcttacaa gaccattcag aagaaaaaga cattgaacgt gtttgagaac atgaaaatca
    10681 aaaccttttt gatcaatcaa ttaatcaatt tgaaataaca aaaaatctga gacagaatga
    10741 agttcattct ggcccagatt tgattttgca caactcatca gtccttattg acaaagagcc
    10801 aattaattag caacgatatt gactaacttg tttggtaccg caataacttt tctgactgtt
    10861 ttgccttcaa tatattcttg cactatttca ttggctaatg ctgctttttc tagctcatct
    10921 tttcctaagt ctcggggaac attgcttttc gcacgaactt taccgtttac ttggaaaaca
    10981 acttctactt cgtcttcaac aagtgctgct tcatcgtatg ttggccatgg tgcgtaagaa
    11041 ataccaccat catttccaag gattgcccat agttcttcac ccatgtgagg agcaatcggt
    11101 gcgagtagtt gaacaaatcc ttcgatatat tcataaggca gagcgtccac tttataggct
    11161 tcattgataa agaccatcaa ttgggagatt gctgtattga aatgaagatt ttcatagtct
    11221 tctgtcactt ttttcactgt ttgatgatac actttatcaa gcttgccatc attcaatgta
    11281 gtgatacggt cacgcatttt attgttctca tcaacgatca aacgccatac acgatccaag
    11341 aatttacggc ttccttctaa gccattttcg ctccaagcaa tcgaagcatc taatggcccc
    11401 atgaacattt cgtacatgcg cagtgtatct gcaccgtaag tttcgaccac atcatcagga
    11461 ttgactacat taccacgaga ttttgacatt ttttcgttgt tttcgcctaa gatcattcct
    11521 tgattgtata gtttttggaa tggttctttt gtaggaacaa caccgatatc gtataagaat
    11581 ttatgccaga atcgtgcata tagtaaatga agtaccgcat gttctgctcc accaatgtag
    11641 atgtcaactg gtagccaacg cttcaacttt tcataatctg ctagttcttt tttgttatga
    11701 gggtcgatat aacgcaggta ataccatgag cttcctgccc attgtggcat tgtattcgtt
    11761 tcgcgacggc cttttttacc tgttacagga tcaacgacat tgacccactc ctcgatattt
    11821 gctaatggag attctccagt accgcttggc ttgatgtttt ttgtcttagg caaccgtaat
    11881 ggtagctctt cttctgggac agctgtgact gttccgtcct cccaatggat gattgggatt
    11941 ggttcacccc agtaacgttg acgggagaat agccagtcac gtaagcgata gctgacttct
    12001 tttttcccta cgccattttc ttctagccat tcattcattt tatcgattgc ttcttgtttg
    12061 ttcatgccgt ctagaaattc agagttgatg tgcgtaccat cttctgtgta agcagctttt
    12121 tcaacatctc caccttcgat aactggaaga atttctagtc cgaaagtttg agcgaattca
    12181 aagtcacgtt catcatgtgc tggaacagcc atgatggctc ctgtgccgta tgaagataat
    12241 acataatcgg cgatccagat tgggatttcc ttgccattaa ctggattgat tgcataagca
    12301 ccagtaaata ctcctgtttt ttcttttgca agatccgttc tctttagatc tgatttttta
    12361 gctgtctctt cgatataagc ttctacagca gctttttgtt caggggtcgt aatttctcta
    12421 acaagatcta gttcaggtgc aagaaccgag taagttgcac cgaatagagt atctggtcgt
    12481 gtcgtaaata cggtatagga tttatccgta ccagccactt taaattcaat atttgctcct
    12541 actgaacgac cgatccaatt tctttgcatt tctttaatgc tttctggcca atcaactaat
    12601 tctagatcat ccaataagcg atcagcataa gcagtgattt tcagcatcca ttggcgcatt
    12661 ggttttctga ctacatcata gccaccacgt tcacttttcc catcgatcac ttcttcattt
    12721 gaaataactg ttcctaattc aggtacccag tttaccgcta cttctgcttc ataagctaga
    12781 cctttttcat aaagcttcgt aaagatccat tgtgtccatt tataatactc cggatcagtt
    12841 gtattcactt cgcgattcca gtcataacta aatcctaaag aattgatttg gcgtcggaat
    12901 gtttcgatat ttttttgggt aaactcagct ggatcgtttc ctgtatctag cgcatattgt
    12961 tcagcaggca gaccgaatgc atcccacccc attggatgca gcacgttgta tccttgactt
    13021 cttttcacac gtgaaaggat atccgttgcc gtataacctt caggatgccc tacatgtagc
    13081 ccttgaccgg atggataggg aaacatatcc agtgcgtaaa attttggctt ttcaggatca
    13141 tcatgggtgt tgaaagtatt cgtttttgcc cagtattttt gccatttttt ctcaatttct
    13201 ttatgattgt aactcatttg ctgtcctcct gttctattga aaacacttgt ttctattata
    13261 aatataacga aaaaaccggc agaatctgtt tatttttatt tccttttgct ttgttttctc
    13321 aaacttcaca aaagaaataa aaaaatccta taaaaagatt gctcttttta taggacgtaa
    13381 tctacgcggt accacctaat tttaccttag tgaaaaggcc tcgaacgttg taacggccgt
    13441 caccgccgct tgcttactcg ggtattcagc aagggcaact caaaggcgag ttcaaaagca
    13501 tccgtgcaca ttctcaccag ccatgcgctc tctgaaactt cagtcttttt actacttcct
    13561 tttcaagtgt tgtcatattt aagtagcctt atagtaacaa atatttttgt ggaattcaat
    13621 cgttatttct tcgaacagct attttattta gctacaatca acgtttgacc tggataaata
    13681 aagttgttct taatattgtt ccattcgatt agttgagcca ttgtaattcc atgactgtca
    13741 gcaacactcc aaacagattc tccagatttc acagtatagg ttttcgaacc agtaactgat
    13801 tgttggtttg ttgattgaga tgaacctttc ttcacaatca cttgctgtcc tggatagatg
    13861 aaattatttt tgatattatt ccattcgatt aactgatcca tcgtgatatg atgtttgttc
    13921 gctacgctcc aaactgactc gcccgctttc acggtataca tcgtattacc agtggatgat
    13981 gtacttgtcg tagcagacgt attcggtgta tttggtttcg ctgcagaagt agttgaacct
    14041 gtatttgtat ttgccgaagt tccaccttta acgatcaatt gctgtcctgg ataaatgaag
    14101 ttgtttttga tattattcca ttcgattaac tgatccatcg tgatgccgtt tttatttgcc
    14161 acgctccaaa cagattcacc tgctttcacg gtataagtgc cttgagacga actgctgcca
    14221 gtattaactg aactgctgtt atttgtattg gttgacgaac ctgtattacc tgacgtgctg
    14281 tttgttgatc cactgtttga aacagtcagt tgttgtcctg ggtagataaa gttattttgg
    14341 atattgttcc attggattag ctgagccatc gtaattccgt atttatttga gacgctccaa
    14401 acagagtctc cggctttaac tgtatatttc gttcctgaag ctgtattaga agaacttgag
    14461 ctggacgaac tagagccact tgtattccca gtatttgagc cagtatttgc tccagcatta
    14521 gaagaaccgc catttgatac gattagctgt tgtcctggat aaacgaaatt gttcttgata
    14581 ttgttccatt caatcaattg agccatcgta attccgtgat catttgagat cttccaaaca
    14641 gaatcaccag attgaacagt gtatttcgca ccagttgttt gtcctccgtt tgatgtacca
    14701 gcatttcctg aagagttggt attgttaccg gaattattcg tcgaagagcc attttcctgg
    14761 ctacctttga ttgtcagttt ttgtcctgga taaatgaaat tgttttggat gttgttccat
    14821 tggatcaatt gatccattgt gataccgaat ttatttgaga ttccccaaac agaatcacca
    14881 gattggacag tataagtggt cgaagttgct gaacctgagt ttgtattacc agaattgctt
    14941 gaaccgccag tactacctgc acttccagta ttattgccac ctgtagtcgc tccaccgcct
    15001 gcgtttccgc tagctggtgt atcatattgg gtcaatccgt aagttgcgat gatattattt
    15061 aatttgcctg cgtaaccagg atcagtcgca taacgccctg tcaaacaagc tgtcgcatct
    15121 gtataggatt tcgtgttgct cttccaagta ccggcatagt aataaccgtt gccaaatgaa
    15181 gtatttctaa gtacataggc attgtcattg aaagattcag caaaagaagg atattgtcgg
    15241 aaaggctcct ttttatttac ccattgatta tttaaaaatt caagcgtatc catgtagacg
    15301 gtttgtcctt gatagctgcc ttttatccca aaaagattat aataaggtgc ttgagataac
    15361 gcactactgc cccaaccgct ttctacaact gcttgtgcga tcatcactga tgcatataga
    15421 tcgttggctt gagcgattgg ttgagcataa gtagcaattt ctgtgataaa tgccgctgta
    15481 ttgattcttg cgccgaactg aacaggtgtt tcatccgctt gaacaggaac cggctgtact
    15541 aatggggcag cgacagaaca gcctaccatc gcagttccca cgattgctgc ccctcgtttt
    15601 gcttttcgaa aacgtttggt ctgttctaaa cgtctccgtt cttttcttgc aatgttttcc
    15661 atcttctatc ctccaacttc aatttatcta ttattaataa ttataacttc tcttaatatt
    15721 tattaaaagt tatttaagag ctttgttgtt ttttgtaatc atattgtaat ttaccgtaat
    15781 gaacaacttc tgttcatttt caagttcttc ctacgtctac gttcttgttt aaaaggattt
    15841 cgaaaaacac tgattttatt tccctaacat ttcatttgca ttaccaaaaa atgtctttcc
    15901 gattacagtt atttttttta taagaatatt ggttattcaa agaggaatca tacattggtt
    15961 ttatctcttt accgtttttt tcgctggacc aaaaatgcta taataagtca ttgcttcaaa
    16021 ggaggaattg tatgaaatat aaaacatcat ttcaggtggc tggtagcttc tttcttgtcc
    16081 tgtttttcat tttgggctgg atcgtaaagt tccatctgtc gtggctttct ggtttcgatc
    16141 agtggtttac aggcattgtc agagcttctt atcctgatct gaacagctac caactcttta
    16201 tgactaaatt cggtaatcct cttaccgtca tcattttatt tgcttgtgcg gctttatgga
    16261 tgtggtataa gaaaaaaagg gcagaaacat tttggttcgt ttctggattt attgtaattg
    16321 ctggaatcat caacccggtg atcaaactat ttttcatgcg agagcgtcct tcattggcgc
    16381 acttagtagt tgaaacaagt tacagtttcc ctagtggaca tgcagcaggc agtatgattc
    16441 tttacggttc tttactattt ctgcttccag cttttgttca aaaaagaacc ttacgcacat
    16501 tgcttcaatt gctgatgatg tttattatag taagtgtcgg gataagccgc atttatctgg
    16561 gcgttcattt tcctactgat atcatcggcg gctacttact cagtctttct tggctgtgcc
    16621 tgacttatcc agtgtataga gaaaaaagaa aacaaataga taactcttac agaaagaagg
    16681 acttttaatg acttttcctt attcagaagg aaataaaaga tatcattctt ggaattacgc
    16741 tttacgaaat gagtttggcg ggaaaatttt caaggtgccg atcgatggag gatttgactg
    16801 tccgaatcga gatgggactg ttgcccatgg cggttgtact ttttgcagtg tatctggttc
    16861 gggagacatg atcgttgcac ctgaagaccc tcttccaatc caattccaga aagaaatcga
    16921 catgatgcat caaaaatggc ctcatgtcca acaatacatc gtttatttcc aaaattttac
    16981 aaacacccat gcgccagttg aaataatcaa agaacgattt gagcaagtag tcaatttacc
    17041 aggtgtcgta ggattatcag tcggtacacg accggattgt ctgccagatg aagtagttga
    17101 ttaccttgct gaactgaacg agcggatgta tttatgggta gaacttggat tgcaaacaac
    17161 ttttgaagaa acttcaaaac tgatcaaccg agcacatgat tatcaaactt atttagatgg
    17221 cgtggctaaa ttacgcaaac ataatatccg tgtttgtacg catttgatca acggattacc
    17281 aggcgaaagt ttagaaatga tgaaagaaaa tgtcagacgc accattttag attcagatat
    17341 ccaaggaatc aagctccatt tgatgcatct gatgcgcaaa acgagaatgt tgcgggacta
    17401 tcatgaaggt cgccttcaat taatgagccg tcctgactat gtcaatgtca tttgcgacca
    17461 gctggaaatg ataccaaaag agatcatcat ccatcgtttg acaggggatg cgccgtggga
    17521 ttccttgatt ggaccaatgt ggagcttgaa aaaatgggaa gtcttgaatg caattgatga
    17581 agaacttctt cgcagagata gcttccaagg aaaatatgat gtacggaaaa aggtgagcgt
    17641 ctgatgttgc aaactgcttt gcatttcagc catacattac tgcaagaaat cttgcaacca
    17701 ggggatcacg tcgtagatgc aacgatgggc aatggccacg atacggtttt tctagcagaa
    17761 cacatcggaa aaactggtca tgtctatagt tttgatatcc agcaacaagc aatcgatgct
    17821 actcgtgaaa ggctagaaca gcgtcagtta gaagaacgtg tctcattgtt tctacaaggc
    17881 cacgaaacgt taggagaagt gatagctgaa caacaaaatc tcaaagcagg gattttcaat
    17941 ctagggtatc tcccaaaaag tgataaagca atcatcacta tgccagagac tacacgcaca
    18001 gctatggaag aaatattgaa acgacttgtg cctcgcggac gattgatcct tgtcgtctac
    18061 tatggtcatg aaggtggaga aaaagaactg gatatggttg attctttttg ttccaaactg
    18121 ccacaggaaa catataatgt gctgaattat cggtttatca accaaaagaa ccagccgcct
    18181 attttatatt gtattgagaa aaaaagataa ataaaaaacg caaaacataa atgatatgtt
    18241 ttgcgttttt ttctctaata ctcaggttag agcgttgatt tcataacctc tttattcacg
    18301 gtgctcaaaa gcttatatct gcggtaataa gtccaaccaa atcaaaaaca tgagaagctg
    18361 tttttgattg gctgttctta ttacttgatg caggacagct ttttcacaac ctctttattc
    18421 aaatacaaat tgaaattgaa cacatttttt tgacatataa cagatatata aaagaatggc
    18481 aaaaacattg atatataagc atttttaaaa gattttatat ttttgtatgt cattcatata
    18541 tcaatagcta acttaatatc agtcaaatta tgcccctttt ttgcccctca aatatcgctt
    18601 tacaacaaga acgtaagttc gtataatcgt tttgaggtga tttttatgat ggaagaattt
    18661 attagaaaaa atatcagtga tgaatatgca gacttttatg atcaaagcag taaaaaagat
    18721 aaattccaga tggatgtttc aatcttagct atattagctt tttccgaaaa taaacaacct
    18781 gtaactgcaa aaaaagaaac agtattctct gaaggcaaaa taaaaactcg atatatatta
    18841 gaggtagaaa ctaattttaa aaacagatcg gagtaatggt tatgcttttt aatgag
//
LOCUS       DACEDD010000057        18504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEDD010000057 DACEDD010000000
VERSION     DACEDD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.057"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IWO45_002222"
     CDS             99..746
                     /locus_tag="IWO45_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0333682.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IWO45_002223"
     CDS             1139..3784
                     /locus_tag="IWO45_002223"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0333683.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FYRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IWO45_002224"
     CDS             4094..5407
                     /locus_tag="IWO45_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0333684.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IWO45_002225"
     CDS             5397..6050
                     /locus_tag="IWO45_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0333685.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IWO45_002226"
     CDS             6105..6797
                     /locus_tag="IWO45_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ0333686.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IWO45_002227"
     CDS             6947..7147
                     /locus_tag="IWO45_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0333687.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="IWO45_002228"
     CDS             complement(7401..9080)
                     /locus_tag="IWO45_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0333688.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="IWO45_002229"
     CDS             complement(9082..9294)
                     /locus_tag="IWO45_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0333689.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="IWO45_002230"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="IWO45_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0333690.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="IWO45_002231"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="IWO45_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0333691.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="IWO45_002232"
     CDS             13269..13466
                     /locus_tag="IWO45_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333692.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="IWO45_002233"
     CDS             13619..14104
                     /locus_tag="IWO45_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0333693.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IWO45_002234"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IWO45_002234"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0333694.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="IWO45_002235"
     CDS             14981..15508
                     /locus_tag="IWO45_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0333695.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="IWO45_002236"
     CDS             15599..16336
                     /locus_tag="IWO45_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0333696.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="IWO45_002237"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="IWO45_002237"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0333697.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="IWO45_002238"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="IWO45_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0333698.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4021 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta ttttatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DACEDD010000058        18123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEDD010000058 DACEDD010000000
VERSION     DACEDD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.058"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            283..882
                     /locus_tag="IWO45_002239"
     CDS             283..882
                     /locus_tag="IWO45_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333699.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            994..1218
                     /locus_tag="IWO45_002240"
     CDS             994..1218
                     /locus_tag="IWO45_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333700.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            1570..1806
                     /locus_tag="IWO45_002241"
     CDS             1570..1806
                     /locus_tag="IWO45_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333701.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            1886..2740
                     /locus_tag="IWO45_002242"
     CDS             1886..2740
                     /locus_tag="IWO45_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0333702.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            2753..3190
                     /locus_tag="IWO45_002243"
     CDS             2753..3190
                     /locus_tag="IWO45_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333703.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            3208..3507
                     /locus_tag="IWO45_002244"
     CDS             3208..3507
                     /locus_tag="IWO45_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333704.1"
                     /translation="MDHKSNYSYKENYIWKYVVAVFLLVGGIIALVKWAIIPNALVFI
                     NWFVPNFWSFFGYILIIWFMFKVLSFFWSPVYSLLGKWSYLILIATAIYIIMQIN"
     gene            3510..3953
                     /locus_tag="IWO45_002245"
     CDS             3510..3953
                     /locus_tag="IWO45_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0333705.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            4098..4421
                     /locus_tag="IWO45_002246"
     CDS             4098..4421
                     /locus_tag="IWO45_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333706.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            4839..8468
                     /locus_tag="IWO45_002247"
     CDS             4839..8468
                     /locus_tag="IWO45_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333707.1"
                     /translation="MKKLKLISIALFSLITMSTARQSIVSAEGTSTKAVVEESGDKKV
                     AIDDKVKNEAEKATVVQGADGLMYVVVDENDPEKLLEAESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFESE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRHALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVVFTIYNSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEISQDTPFTKPAKVKVT
                     NIYEVSISEKVSDMNESQVLKNNWSADPNDYLQYDITTGNLKKSGERNVQSFIIEGFY
                     DNKNVDVSKSEVYVGSTNVTNSFDINNDTNNGKVIAKAKTSVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKNLTIVKNSKVTVGSKISKSASSNDVETVLYSRKVTINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDVIHKGKIDGKDIVLNTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTKIELSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMSLDLPTEYLTKNKKINYSVDILLVDNPDKNKFETETKDLRTIGYTSS
                     EKVLTNKDMTNNQVNYSAVVRTVKERENNNPVKEFNESINYKFTDSTKTKTGYGISVD
                     LEPTYRNEIGKQIDMKVYSYPMNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGI
                     STQNLSFQFPHMNIERKTGNIYADSQRNDESIKYELIDGGRKLYVPIWCDLGNYSIAI
                     KSSNFGGNYISLDMNQNIDVYAYMYATIDSETKKDDELLLEPVYPESTLPDGWSKNEI
                     DWLNK"
     gene            8528..8890
                     /locus_tag="IWO45_002248"
     CDS             8528..8890
                     /locus_tag="IWO45_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333708.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            9124..9546
                     /locus_tag="IWO45_002249"
     CDS             9124..9546
                     /locus_tag="IWO45_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333709.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            9558..10973
                     /gene="cpaF"
                     /locus_tag="IWO45_002250"
     CDS             9558..10973
                     /gene="cpaF"
                     /locus_tag="IWO45_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0333710.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            10990..11805
                     /locus_tag="IWO45_002251"
     CDS             10990..11805
                     /locus_tag="IWO45_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333711.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            11805..12602
                     /locus_tag="IWO45_002252"
     CDS             11805..12602
                     /locus_tag="IWO45_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333712.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            12620..12982
                     /locus_tag="IWO45_002253"
     CDS             12620..12982
                     /locus_tag="IWO45_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ0333713.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            13154..13375
                     /locus_tag="IWO45_002254"
     CDS             13154..13375
                     /locus_tag="IWO45_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333714.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            13394..13669
                     /locus_tag="IWO45_002255"
     CDS             13394..13669
                     /locus_tag="IWO45_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAQ0333715.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            13778..15604
                     /locus_tag="IWO45_002256"
     CDS             13778..15604
                     /locus_tag="IWO45_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333716.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            15617..16333
                     /locus_tag="IWO45_002257"
     CDS             15617..16333
                     /locus_tag="IWO45_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333717.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKR
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            16346..>18123
                     /locus_tag="IWO45_002258"
     CDS             16346..>18123
                     /locus_tag="IWO45_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0333718.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHF"
BASE COUNT         6896 a         2249 c         3457 g         5521 t
ORIGIN      
        1 tttttattca aaagtgccca aaatataggt atactgaatt aaggataaat acaaaactag
       61 ttagaggaca gttgaaaaaa tacaaacaaa ttagattgct attttgttaa attggaagta
      121 gaataatacc gttttgatat gtatagtgtg ttttaccaat aaaattacat aaacgtgaat
      181 atatttttat gaattaaatg taaaatgaga gcaattagtt ttaaaaaaga ttataggctg
      241 ttaatcttag catatataat ccattggaaa gggggagatc caatgaatct aaagaataca
      301 aactcaaaca aatctacact atatgtaatt tcagatgatt atataaataa gctgaaaaag
      361 gtggactatc gtgtccaaaa caactatcga gaacaacgat tatattttaa atcaaacttg
      421 aaaatttcta acaaaaatga agaaataaat tattatgtac cattggcttc agcaaaagaa
      481 aatcaacgtt ctttgactaa taaatcagtg tttaagattt atggtgatcg agaagaaaag
      541 gactttttag gagtacttca tataaataat atgattcctg tgccaaaaga ttgtgcaaaa
      601 gtatggacac ctaatagccc aaatgttgat gagaagtata agatgttagt aatcaaacag
      661 gcaaaatatt tgaaattaca tgagtctgaa ataaatgaat catgcgaatt actttataat
      721 tctaagttag gtaaacttga tcctagttac tttaagaaaa atcgtaaaga agtaattact
      781 tatcaaaaaa taatgacgaa tgtagaatca ttggaagctg ttagtatagc agaatcccaa
      841 agaaatattc atgtacaaac tcaaagtata gatgtagagt aagataggct aaacctatct
      901 tattttatta ttctattgga tattatgata cattgaattt taatcgaata tgtatttaaa
      961 tgttattata ctgtagaaga taaaacttaa aggatgattt ctatggcgag accgagaaaa
     1021 tacaatacag aagaagaaag aaaagaagca atgaaaaagg ctaggaaaaa atgggttgaa
     1081 aaaaatcagg aaactattgc ctatataaga gccaaatcat ttgctaaaaa attcattgaa
     1141 atgtctgaag atgaagatat tgaattgttg gaaaaatggt tagaagatcg gaaaaataac
     1201 ggtcaaaatg atgaataata accattgact acaagtagtc aatggtgtag aatggtttta
     1261 gggatagaaa tatccctttt ctaaaagtct aatgactaca agtagtcgat agagggatcg
     1321 ggaataaaaa cagacgatta tggcctaaag aaaggaggaa tatgaatatt ttgaggaacg
     1381 atttattaaa tgagcgttga taaatttcac taaatatttt cttaatatct atgtattgtt
     1441 caagcgtaga aaaaaatatt gaaattatta caaaaccgct cataatggaa aataacgaat
     1501 tatattaatg aatcgttcac ttttgacctt ttttactaga tttttttgta ggacttttag
     1561 agaatttttt tgttatctga ttataaggag gagctaaagt tgatagagaa tagagtactg
     1621 gggttcgtag agtgtaaaga taatttagac gatactttag aagtccttga aagtattata
     1681 tttaacacca aagttctgga tttgaaagca attaatggtg atatgacaac acatatcata
     1741 ttagatgaaa ttagcgcaaa agagattggc gaaactttaa tagcatgggc taataaagag
     1801 ctgtaaaaga taattttaaa ataaagtggc caattgacta caagtagtct gtatagtata
     1861 ataaattatc aaaagtgagg tatacatgat gaaatcaaat tttttgggat atgtaattgc
     1921 aatcaacatg tttaaagggg gagttggtaa gtcaactctt actcagctaa tggctgatat
     1981 tctatcggct agaggcttta aagttttagt tattgatgtt gatccacaag gaacagtaac
     2041 taaaaagttt caaagacaat atgatttaaa agttgttggg aaaatgacat tgtttgaagc
     2101 agtaattaat atgtctttct tagactgcat ttctcctgtt aaagaaaact tagatattgt
     2161 gcttggttca gaacaaatgt caaggtttac caaagtatta agagatatgt ggagaagtac
     2221 tggttttact gatgaaggtg aagaagtctt ttggaatttg tttaattata tgatcggtaa
     2281 tgataattta agagatcaat atgattttat tttgtttgac actattccta cagtttcaga
     2341 atttacagat aacgtttacg tggcgagtga ttttttagta attcccacgc aaactgagca
     2401 agattctata gacaacttgc atagtacggt tactaattat caaactgctg taaaagaagt
     2461 aaacggagat ttagaaattt taggtattgt tccatatcta gtggatacta aattaaaaac
     2521 aggattagaa cttttatcag aacttcaaga agaatatgga gacttagttt attctcatat
     2581 tgtttcagaa cgaggcgtaa ttaaacgttg gggtagagaa ggactaaata aatctatccc
     2641 acattctaaa actgcttata gcatgtatgt agacattgta gaagaaacgt tagtacgttt
     2701 agaaaaagct acaggagaaa atattttaga acgtgtttag gaaaggtgtt gcatgatgtc
     2761 gaaaactaaa aaagtaacta gtgccggcag taatggttcg ggtaatccgt tcggaataaa
     2821 aagcggtagt ggtggattta atataggaca acaaatgtta aaaggtaaag ctggcattaa
     2881 gtctactggt gataaacgga aatctgttag ggtaccagtt gaattatacg atgaagttgt
     2941 taaagattta tcatacgggg ctaaatattc attaatgaca gaaattttag ctttattttt
     3001 aaaagaattt gaaacacatg gaaatagtat agttactgat tttggcaagt tgaaaacgag
     3061 accagtaaaa atttctgaag aagcctttaa agttttgaaa gttataaaat ttgaaacaga
     3121 tcaatctaat actaatatta ttgcttcagc tttgacatgg tatcttgata aaagaaaagg
     3181 aaaagcataa tgaaatgagg taagactatg gatcataaat caaattattc atacaaagaa
     3241 aattatattt ggaagtatgt agtagccgtt tttctactgg taggtggaat tattgcattg
     3301 gttaaatggg ctataattcc taatgcttta gtatttatca attggtttgt ccctaatttt
     3361 tggagttttt ttggctatat attgataatc tggtttatgt ttaaagtact ctcttttttc
     3421 tggtcgcctg tatattcttt gcttggaaag tggtcgtacc taattttaat agcgacagct
     3481 atttatatca ttatgcagat taactaatta tgcagatttt agaagatatt aaaatttatt
     3541 ggaaaaggat aataggctat acattagttc ttcttgtagt aaatttattc ctttcgatga
     3601 tagcgttggt ctttgttaat caaagaaaag acaatgtaac tgaattagta acgtttattt
     3661 cgtttttcat aagtgggatt tttgctcctt tctttgaaga aaccttaatg agaggaatta
     3721 tccaaaacca attggaatta aaaacaaatt tgtctacaag aagcatatat ataattgttg
     3781 caattttgtt tagtctatcc cacttaaatt tatattttat tccttatttt atgacgagtt
     3841 taattttgtc ttttgtctat gataattcag ataaaaggct gattgttccg gttactattc
     3901 attgttttta caatttaatt gtaatactgt taagtgtagt tttttatact tgaaaaaaat
     3961 agacgaaaac ctaaaaatac aaggttttta tctatttttt ttattttgtt tttatctatc
     4021 aggttgatag aaattgtcat aaatgatata attaagtatg aactgattta gtaaaaaaat
     4081 taaaggagtg atagctgttg gcaagatcaa gaattgctaa aattgaacag actaaaaagg
     4141 ataaaaaaat ttctaaaaat aaaacaaaat tctttaaagc tgaagatgtt aagaggatag
     4201 catggtctgt gaaaacattt gatgaagatc gttatgttaa agtttcaatg aatggtctat
     4261 ttgtacagct tggtaacttc agtaatttta aaacgattga tgaactttat ccggaaataa
     4321 gaaatattgt ggatagtaaa ggtttaatcg tggctaaacg gaaatcaaga aactctggtc
     4381 ttgcttactg taaaaataaa gggatcatga ttccggctta aatcagtgaa attgttcttt
     4441 gaaaacttaa tactactcgt actgtagcaa tgttgctatg ttacagataa aagcgtaagg
     4501 ctaataagca aaatagcaac caagcatact gttatttcct ttgccaagag gtatgtaaat
     4561 acgttagcaa tagaagtgat aaggttagta tttcacgcca aaaaatacaa aagtcgatcc
     4621 attgaaacta ctaattatga ggtggcgtaa atggtaatta ttcagaattt ctatctagga
     4681 agaacctatg aaaaccgtcc tagagatcag aatattgaca ctaaaaaatt cagcacagta
     4741 ataaatgtgc tagatacagg gtaactcttt actacaagaa atgtagtaaa gagttgttat
     4801 gattctttat tacatttctt tctaaaggaa gtgtgataat gaaaaaacta aaactgatta
     4861 gcattgctct attcagccta atcactatgt caacggctcg ccaatcaatt gtttcggctg
     4921 aaggaacgtc tacgaaagca gtagtagaag agtctggcga taaaaaagtt gctatagacg
     4981 acaaagtgaa aaacgaagcg gaaaaagcga ctgtggttca aggggcagac ggcttgatgt
     5041 atgtggtcgt tgatgaaaat gatcctgaaa aattgctaga agctgaatct cctttgaaaa
     5101 atcaacaaga aaataccggc gctgttgaag tcggtgttga tgaaaagaga caagaaaagg
     5161 ctgataaagc taacgaaaaa gtgaaaaact ttgacgatat tgattacaac gatattagtt
     5221 cgttgcagtc agcgttgaat gatccgaaat tccctttttt atcggaagat gaattaaatg
     5281 aaaaattgca aaagacaaag aatacatttg agtctgaagc gctaatgaaa aagacaaggg
     5341 gatatttcag tcaaaggatt ttagagttaa gcaataattt aaatgacgta ccgaaaagag
     5401 cgaaagtgag tcggcatgct ttagttagta accgtgcttc acaagtaagt gaaacacgaa
     5461 taaaccctcc aaatggttat ccgtgggtga tttatttaat taggagcaat ggttatagtt
     5521 atacagaaaa ttatgctatt agaagaacta ttgatgggaa agaagctttt tgtttacagg
     5581 ccggtgtaca agcgacgaat acatacactg gttcaacaag tggttatctt tctaattcgc
     5641 aaagagaaat gattagttcg attttatcag catataaaga attaggcggt tgggctaata
     5701 atgatgccgg aaaacaaaga tggatagcaa ctcaaatgct tgtttgggaa aacagtaatg
     5761 aaaagcctaa aagtataact gttacgccaa attggaaaaa cggagctact aagtggaaaa
     5821 atgatttagt ggctcgtgca aatgctatta gagtaagaac aaaatgggat tctacgtcta
     5881 agacggttga tgagggagat aaagcaacct ttaaagtaac taatacttat ggagatcata
     5941 atttgcatgt gaaatctgta tctggtggcg gcaaagctac cataagcaat ggtactttaa
     6001 ctgttgatac tacaggagcc actggaaaca aaatcactgt agatatgatt aaaggttctc
     6061 acccttcaga cgcgcaccct acaacggtat ggagtaatgg ttcttatcaa aagcttgtta
     6121 ccggttcttt ttacattagt acttctgtta cggtaaatgt taagagaaaa ggaactgttg
     6181 aagttttaaa agttgattca gaaacaaaaa aaccattatc aggagcaaca tttagattta
     6241 gctactcagg taaaacaaaa gatgttacta ctgattcgaa tggtaaagca aaattaactg
     6301 aaaggttgaa agctggaact gtagtaaaag ttaaggagat aaaagctcct aatggctacc
     6361 aactagattc aagcgaattt tcaattacag ttaaagaaaa tcaaaatgtt actaccacaa
     6421 gaaccaataa gaaatccaca ggttctgtag aaattgaaaa gataggcgat ttagatggat
     6481 taggactacc gaatgtagta ttcacgattt ataattccga taacaaagtt gtaaaagata
     6541 acttaaaaac agatagtaat gggaaaataa aagtcgatga actgcaattt ggtaaatatt
     6601 atgcagtaga aaaacaaggt gttactggtt atgatcccga tggtaaaaaa tacaattttg
     6661 aaataagtca agatactcct tttacaaaac cggctaaagt gaaagtaaca aatatttatg
     6721 aagtatccat aagcgaaaaa gtatcggaca tgaatgaaag tcaggtatta aaaaataatt
     6781 ggtctgctga tccaaacgat tatcttcaat atgacattac aactggaaat ctaaaaaaat
     6841 cgggagaaag aaacgtccaa agtttcatta tcgaagggtt ttacgacaat aagaatgtag
     6901 atgtgtcaaa aagtgaagta tatgttggat caacaaatgt aacaaactct tttgacatta
     6961 ataacgatac taacaatgga aaagtaattg ctaaggcgaa gacaagcgta ttaacgaata
     7021 atgattttta taataagtcc tataatttac ggatcactat gaaaattaaa aaatctactc
     7081 ttgaaaacac tgaaaagcaa aaaaatttaa cgattgttaa aaatagtaaa gttactgtcg
     7141 gttcaaaaat ctctaaatct gctagctcta atgatgtaga aaccgtttta tattcaagaa
     7201 aagttactat caatcatatt gatgaaaaag acaaacacct tttgaaacaa gatattgatt
     7261 ataaatacga tggggaaacg tatgaatata aacctagaac cgatttattt gataaagatg
     7321 gaaacaatta taaatctgat gtaatccata aagggaaaat tgatggaaaa gatatagttt
     7381 taaacactcc ctatcatatt ccggtacttt ctgtgaatgt ggatcggatt caaattgata
     7441 cggctagagc tgttaaaaat ggctatttac ctacaaaaat agaactaagt aaaaaagaag
     7501 aatatgaaaa agaattagaa aaaattgtat tcaaagttaa aattacagat atagataaca
     7561 ataaagttgt ttatgatgaa aactttaaat acaaagaatt taatagtcat atgtcgcttg
     7621 atttacctac agaatattta acaaagaata aaaaaatcaa ttatagtgtt gatattcttt
     7681 tagtggataa tccagataaa aacaagttcg aaactgaaac taaagattta agaacgatcg
     7741 gttatacttc ttctgaaaaa gttttaacaa acaaagatat gacaaataac caagtcaact
     7801 attcagcagt agttagaaca gtgaaagaaa gagagaataa taatccggtc aaagagttca
     7861 atgagagtat caattataaa tttactgata gcactaaaac aaaaacagga tatggtatat
     7921 cagttgattt agagccaaca tatagaaatg aaattgggaa acaaatagat atgaaagtct
     7981 atagttatcc gatgaatgaa ttgattgaca gcaaaattga cgaaatctat caaagtgggg
     8041 gaaaagaaac agaagttaag atggatcgct taaagaatga tggtaaagtt gagtcgggta
     8101 ttagtactca aaatctttca ttccaatttc cacatatgaa tatcgaaaga aaaactggaa
     8161 atatatatgc tgacagtcaa agaaatgatg aatctattaa gtatgaattg atcgatggcg
     8221 gtagaaaatt atatgtgcct atttggtgtg atttgggaaa ttattctatt gctatcaaaa
     8281 gtagtaactt tggtggtaac tatatctcgt tggatatgaa ccagaacata gatgtttatg
     8341 cttatatgta tgcaacgata gatagtgaaa caaaaaaaga tgatgaatta ctgcttgaac
     8401 ctgtttatcc agagtctact ctaccagatg gttggagcaa aaatgagatt gattggctga
     8461 ataaataagt ttgaaaagtt tttatctatc tgatagaatt aaataatgat atagggatgg
     8521 tgatagaatg agggttcaaa gctatggtaa cagtgtcttt gatgttaaaa gagaatttca
     8581 agattctaga agaaaaacca aaaagctgat agcttacttc attctatcag ctatcgtttt
     8641 ctatattgtc aagtttgttt tggaattaaa ctcaatagta ttcacagata tggttcataa
     8701 aatgttaaat ggagaaccta ttaatttcaa ctttgattac atgcttgaaa aagcacaaag
     8761 caatcaaatt gtggctttta tcgtgagtat ctattatttt attgaaaaat ctattttttc
     8821 tttaaaagat tttttctttg aaatgcttac caaactaaca acgttttaca ataaaatttc
     8881 aaaaaattaa ggaggaagtt tgaatgttag atcaaaatac agacagaagt tggtggatga
     8941 ttggtgccgt gatcgtaggt gccatcttaa tcgggacagc taaagtagct tttcccgaag
     9001 tgtttaatat cgtcattaca tttttcaaaa acatgatccc tacaaactta ggatagttcc
     9061 actaaaaaat tcacgtagtt tgatgcgtag aaaaataata aagaatgaaa aaggctacat
     9121 aaagtgattt atgtagcctt ttttttggat ggaagtgatg taatgctaag agatatgccc
     9181 aaaatgattc ttttaatgtt tgttgggttc tttttaattt tagtgattgc gtactattca
     9241 ttaaatggct atacacaaga ttcgtctgtg aaaggtatgc aagaaacgtt aagaactaca
     9301 gcaatagcaa acagggatga tagtattcga gtagaaaaag gacgatttgc tttaattaag
     9361 tctgattttg aaaaagattt taaagaaaaa tttgggaaaa gtaaaaatct aaataatgaa
     9421 accgctgatt ataaatttga ttatcttaca gatgggaagg acggtattaa agcaattaaa
     9481 gtgaaggttg aaagtggatc aaaagaatat caagcaactt gtattttaaa tgttgcaaat
     9541 gattagaggt gtgaaatttg cgtgttgtag aggaaaagaa gatttctcca aaaatcgagg
     9601 aaactcctaa aagtaaaatt aatatcgcaa gtattattaa aaaaaatgga attaaagaaa
     9661 actctgaagc gatagaagga aaccttgatt ttttaactaa gcttttagaa gaagatagtg
     9721 gaaattatga accaccaatt gaagatgcaa aattaaatat gattcgtgat gatttaacca
     9781 gaaactattc ccaagaagtt ttacaagctt taactgatga tgaaattagg cgaaaacttg
     9841 tacaagttgt tttgaaagaa catggtactt tctttaaaaa agataaagag gttgcagaat
     9901 atgtagtacg tgaatgtatt ggaacaggga ttattgagcg aattatgacc aacgatgaaa
     9961 ttacagatgt tggttggaat ggctctcaac ttagcattga aactaactct aacaaatggt
    10021 tgtttgacgg tgataaatta aatatcacgc aagagtatat tgaacgtgtt atttctaaat
    10081 atgctgttgt aaatgatcgg gcttttaata gtggtaatcc tatcatggat ggaatgttta
    10141 aaaatgttcg tatatcagct acacacatag ataattcacc agatggctct acgttttcta
    10201 tgcgtgtggc aaggcctaga ttagcattga ataaagaaaa ctttgaagat tttgctcctg
    10261 attatatttt ggagctattt gagaaaatga tgaaaacaaa agcaaatatc tgtatatcag
    10321 gggagacagg tacaggtaaa acggaacttc aaaaattact gctaagtttt attgataatc
    10381 atgatcgtat gattatgatt gaggacgttc aagaaactca cgctaaagaa ttatttccgg
    10441 ataaagatat tttctcttgg atcactactc ccaaaaaagc aatttcagat ttagtaaaag
    10501 cttctcttag aaataatcca cgatggatca ttgtttcaga aacacgtggt tctgaagctt
    10561 atgaaatgct tcaagcggtt ttgtcaggtc actatgttgt aacgacactt catgcggtta
    10621 atgcacgtgc gatacctaga cgttttgtaa atatgtgtgc aagtggtttt aaaataaacg
    10681 aaaatgcggt tgaggaagac ataaatcgtt atttcgattt tggtgtccat atcaaaaaaa
    10741 cagtggttga tggtaaagtg atccgttatt tgaatgaaat tgttgaattt tctgctagtg
    10801 aaccgactac agtttttaag caaaaattta tcaatggaaa gtttgtcatt gaaaatggta
    10861 gtctttccga agattttaaa gaaaaaatgg ctgaaaaatt tattacgttt gaatttcctg
    10921 aagatcaaac taatacagaa gaatcacttc ctgaagaaga agaggtgatt taagttgaaa
    10981 gtacaggcta tgacaaagcg aaaaggtctc atggatgttt cggaactaaa cgcctatcaa
    11041 gtagcgtatg gtaaaccgtt agattataga gattatttga actatgttgg attaccttgt
    11101 ttgtttatgg gtggcttttc ttttgcttta tgttactact ggtggattgc tttgatttgt
    11161 ggagtaattg gaggcgtata tggctttaaa gttataatgc ctaaaagtat taaacgctct
    11221 tatgaattaa agagtttaca ggaaagaaat aaatttatta acaacatgac tcaaattttg
    11281 accgatgata gtaaaacagt tactagagcg ttatctatag caaaacagcg aacaaaaggt
    11341 gaattaagag aagatattca aatcttagaa tcacgtttac aaggtgctga taaatttcag
    11401 attgctgaag cttttcgtga aatcaacaat aaatattata gtgatgttgt ttttacacaa
    11461 tactttgagc aattggaaac agctatttac gaaggtagaa ataattcaga tactatgaag
    11521 caaataaaga gctatcataa tgatgtgaaa cgtaaaacag aaggttttat gaaaatcaaa
    11581 gacggctatc ttcaagatat gaagcaaatg atgtttattg tagctgtttt tattggagct
    11641 ataacttttg cttttggttt ttctactttc tatgaaggtt ttgctcgatc acctatcgga
    11701 tgggtatttg gatctattta ctttttgatt atggttagct tcatgaagtc atttttcaaa
    11761 cattattttg atgatgaaat aatgagtttg ggggtaagta attaatgaaa gttaaggctt
    11821 taaaaaagaa aagaaaatca agtattagcc ccttgaaaat tacgaaaaca gaacataaaa
    11881 gcttttggat taaattacta ggtgatgttt attatcaaaa attacgtgaa atgaacaatt
    11941 ccaatattgc gattattaag tggcaaaaac aacggttaaa aaagtcattg atttttatcc
    12001 tagtcggatt cttaatgtat ctatttttag ggaaagaatg gtattactta gccggtggaa
    12061 ttgctacttc agtaggtttt tatgtttggc aagcaaaatc aatcaatggt atctataatc
    12121 aatttcggtt tgaacgacac ttgcaattta gtaaatttac acggctattg atcccctatc
    12181 tgaaacaaaa aaaagatggc ggaaatttat atggtgtttt tgcaaaaatc gtaaaaagac
    12241 tagattatga taccgataaa aagctattaa tgaagcttat gcaagaaatg acagatcagc
    12301 ctaacgatat tagacctttt acggaatatg cagaacagac ttctggaaca gacatgtccg
    12361 tattatttat gtctacagtt tatgatattc gacaaggttc ggcagatttg gatgttattg
    12421 aagagttgga tcgtttagct tctgaagagt taatggccgg aatcgattca atcattgact
    12481 ataagtcgag gcgttttgtc ttctttccta caaaaattgc aatgacttca tttattttag
    12541 ttgtcggttt tgcagtaggc gttcttttca tgaacctaaa agatattaac tttgggcaat
    12601 aaaggaaagg agaaagtcaa tgcaagaaca atccgttatg gaaatcatta aatggtttat
    12661 aggtttaatg atgattatgg caatggtttc tataggactt ttttgtgttc aattacaaga
    12721 tattaatact ttcaagcaac aagtaaatta tcaaattgaa cgaaaaggag gtctgacgac
    12781 agaagctgtt gaagcaatcg acaaatattc ggctactaat tatgatagac gttttacaat
    12841 tgaaagcgat aagttaaatc agaaagtatc ttatggagaa gaaattgatt atacgatcaa
    12901 agcaactatt ccaattcaaa ttttaccgct tcctgatgta actttatcgt ttaacggaaa
    12961 tagtgtttct caaataagat gagaggaagg ataaaatgca agagaataat gtttccagaa
    13021 taatttattt gattggcgga ttgataattt tatccttagc tattgccggt cttgtagagc
    13081 attggccgga aataaagtta ttaataagaa acttttacga caataaaatg attaaaatgt
    13141 aggaggtata gatttggaag caattcaaga aaatcgtaag gataatcaag aaacaagtga
    13201 aaaaaacaaa aaaaaaagaa aaaagaaagt agataaattt acggaaatga ccgataaaat
    13261 cattgaaaag aaaggactta aacctaaaga ctttgaccgt caagcaatca aagaagcacg
    13321 acaaaaactg tatgatgatt ttgttaaaga aaacatatca cagcttttag actaacattt
    13381 agggggaaat aaaatgaaaa atttagttat cagcaagttt gcaatgttac caagaatgga
    13441 ttttttcgat aatgtaagta agtttatgtc aaatatgact tcaaaactac aaggaatttc
    13501 actatctgta attatctttt gtattgtggt tactggtttg atgtttcttt tcggagaagg
    13561 gccaagtcgt actgctaaaa aatggttaac ttatatcgtg attggggcaa taattatctt
    13621 cggagcttca actatcggac aaacgatcaa gtccgttggt ggtttctaag gggaagcttc
    13681 ttagaactaa aaaattcgca agtattgcat gaaagagcat tgagtgatta atgctctttt
    13741 ttattttgaa aaactataaa ctaaggagat ttaaactatg aaccaacgat tcgaatataa
    13801 ttttgaagat gtagaaccta agttactgaa aaaaggaaaa ggattaaaaa agaaaaataa
    13861 atatactctc aaacaagcag aagcattagt aggatggatt caacacaatt ggtcagacaa
    13921 gacaacaatt accttgatct gttcagacac agaagaagga acaaagtatg cttttgatgg
    13981 aattgaaatt actccatttt cgattggcgg acaagaaaaa ttattgtttc ctgatattga
    14041 aggacaaatt ttacaagcgc cggacagcat gatttccgat cctaataaac tgattttgga
    14101 tattaacgat caatacaaac acgaaccaga cgtagcagaa ccaacaaaag aatcaaagtc
    14161 aaaattaaat ttgaagctcc cttcagtagt attaccattt gctaagaaga aaaataaact
    14221 tatgaacgaa ttgattgaac cagaagaaac ggatatttat gaagaagaat tagaagaaca
    14281 agaagagatc gaagaattag ttgtgtcttc tgaagaaaca gaagaagtag aagaaattga
    14341 aactgtttct gaaaatgaag aagaaacaga agaaatcttt gactttgaag atgaagaaga
    14401 agtggaaaat gtacagaaag gcgatctatc tacaagtagt caaaatgggt tggaagctgt
    14461 aaatgatcca caagttccac catatatatc ttcacaaact cccaattact ctccacagta
    14521 tcaagcgcct aatgtcttac aaccattaag aaaacacgaa acagtgcagt tagttacgct
    14581 taaagagtac tgtgatttgg gagaagaaat tgaaacttca atttctcaaa tggtcgaaaa
    14641 gttgaaacca gaatctttat tcaaattcat agggattcct tctacaggtc ttacaaatac
    14701 tcgtattgat gaatatagaa gtaaccatgc tatgttgcga ttagctgaag taaaatttga
    14761 aaatttaaga gagcattatt cacggcaaat tatttctttt aaagcagaag ccctagatcg
    14821 tttaagaact gcggtagata ttgcttggaa gaagccttac gatttagatg tgaagaaaaa
    14881 caaggccgaa gagttatcaa aaatggaagc agaagcagaa gctaaattaa atgagttcgt
    14941 gaaacgtcag gaaaacaatg taaatgataa aatcaaaaag tttgagatcg aacaagaaat
    15001 agatttaaaa aactttattg caagacaaga ggcagataaa gcggtattta ttgctacaga
    15061 gaaagatcgg actcaaaatt taattgattc taaagcggaa tcaatcaatg cagatttact
    15121 tgaaacaaaa gataaattta ttgatgaaga aatgtacaaa atgaaaattg aaatgaacaa
    15181 aaatttattt gatggcaagc aaaaaattaa atcagaattg gctactgaac tcgctaaggc
    15241 aaatgataat gtttgggaga atgcgctgaa atttattcaa gaaattcaag atgaaattga
    15301 agctaaaaca ccaatttggg ctgctgaaat caaggagttt aatagtttag aagaacaaga
    15361 acatgaacgt atgaagcaaa ctaaagaact tcaattaaaa gaggaaagta ttgaaatcaa
    15421 gcgtttggaa gctgaaatga atttgaagcg tatggaagaa ttagatcatg aaaataaaat
    15481 gcttaaaatt aaacttgaaa ctgccctttc aaaaaatgag ttattagcta tggaacaaga
    15541 tacaaagcaa gtggtgaatg gatcaaacag agttggtgca cttaatttat ttagtaaacg
    15601 ttaaaggaga acggatatga gtgtttttaa taataaagtt atgttaggga actgttatgt
    15661 gaaccacatt ggtttttacg atggaggaac atacagagtt ttattcttac gtttatctgt
    15721 ttttagttac tcaaaagatg gaaagaggca ttatgaaaat attaatgcac aaatttcttg
    15781 tcctttagat agaaatgggg aaaccacgac tacaagtagt caccctaaag ccttgatgaa
    15841 taaatttatc tttgaaaagt tggatcgcaa tttggttgag gggatgaaga taagtattca
    15901 tggaaatatt cgtggaacag aacaagtgct tcttaacagt ggttggaaaa actttggtca
    15961 attatcgcct ttagaaaaag aagaatatca aaaaattcct aaaaatgaat tagtaacaag
    16021 aaatcaaaca tatatctata tagaagaggt aagtatgcca gaaaaagaag aatttagaaa
    16081 gtctcaatct aaggtaacaa aagaagatgt ggaagttgca gaaccgaatc aagcagatga
    16141 aattttatcc gatgatactg atccagtgaa tgatgaagga gaggttattg aagtttttga
    16201 agctgaaatt atcgaagagg aagaagaaat taaaagtttt gataaaagaa tgtatcgaga
    16261 gaaaccaaaa ggttatgtga attttgaaga tatgtattat gacgaatatt ctaatgaacc
    16321 accgatcaat tagtgggggt caaatatgaa gctagtacaa aacaatgaag tgaaagaaat
    16381 aaaaaacggc tttgttaaaa actcgaacaa aattttggga aagctgaaaa aagtaggtag
    16441 tttcaaaaaa aataaatatc atactggaaa aattgaggga gaagatttac caaaaagttt
    16501 acaagataaa ttgaatgttc cgcttgtatt tataggtgtt tttttaggtt caatagtttt
    16561 tctagcaata acgtacttat tgaacttttt aaaagctttg ttagttgcaa tcggacagtc
    16621 agaaagtggg ctacttggtg tagagttcga tcctaagttt agtttggcat atctaaaacc
    16681 acttggggat acgaaacaat actttatgtg tgctgtggtt gcggtaatag gagtcgctta
    16741 tttaatatat gggaaattaa atcatgaaag tgagaaaaat cttgtttacg gtcaaaaagg
    16801 agattcaaga ctttcaacta tcaaagaaat taagagagat taccatgaga taccagaaaa
    16861 aacagaagtg tatgatggta ttggtggtgt gccaattagt cactatcaag ataaatatta
    16921 tattgataga gacacagtaa ataccgttgt tttgggagct tcacgatccg gtaaagggga
    16981 aacatttgtt gttcctttat tagataatct tagccgagca aaaatacaaa gtaatatggt
    17041 cgtgaatgat ccgaaaggag aattgtttag cgcttcaaag gaaacacttg aaaaacgagg
    17101 ttatcgtgta ttagtgctaa atattgatga tccacttgaa tcaatgagtt ttaatccttt
    17161 acaactagta attgatagtt gggctaacaa ggactatcac gaagctagta agcgagcgaa
    17221 tactttaact tcaatgctct ttgcttcagg aatgggtaca gataatgagt ttttctataa
    17281 atcagctaaa tcagcggtca atgcgattat tttaacgatt gtagaacact gttttaacaa
    17341 tgattgcatt gaaaaaatta caatgtacaa cgtggctcaa atgctgaacg aattaggaag
    17401 cttattttac actgatccaa agactagaaa agaaaaaaat gctttagatg aatatttcaa
    17461 tactttacca caagggaatc aggccaagat tcagtatggt tctacttcat ttgccggtga
    17521 taaagccaaa ggttcgattt tatctactgc ttcacaaggt attgaaatgt ttacaagtga
    17581 tttgttcggg aagttaacaa gtaaaatgtc tattgatttg aaagagatag gctttcctaa
    17641 gtccatacaa tttaaagtca atacgaattt agttggaaag aggattagta tttcattttt
    17701 gagaaaagaa aatgatgtaa ttagattaat taagaagtat cgagtgaaag taaaagcttt
    17761 agggctatgt gttttaaatt ttaatgaata tcttcaggat ggagatatgt tggatatacg
    17821 atatgaagaa gagaaatcga gagcatggta tcgtattgaa tttcccaaaa aacaagcaaa
    17881 tagtcatgat acttctttag tcactaaaaa attcaagtca ggtaaaaatt attctgactt
    17941 agaaatcagc accttacgat taaaatacac ggatcagcca actgctgtgt tcatggttat
    18001 tccagattat gatccgtcaa atcacgtact tgcaagtatt tttatcagtc aactttatac
    18061 agaattagca agtaattgta agcaaacacc ggataaaaaa tgttttaggc gagttcattt
    18121 ctt
//
LOCUS       DACEDD010000059        18063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEDD010000059 DACEDD010000000
VERSION     DACEDD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.059"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..675
                     /locus_tag="IWO45_002259"
                     /pseudo
     CDS             316..675
                     /locus_tag="IWO45_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(970..2103)
                     /locus_tag="IWO45_002260"
     CDS             complement(970..2103)
                     /locus_tag="IWO45_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0333719.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="IWO45_002261"
     CDS             complement(2094..2762)
                     /locus_tag="IWO45_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0333720.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWO45_002262"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWO45_002262"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0333721.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWO45_002263"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWO45_002263"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0333722.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWO45_002264"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWO45_002264"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0333723.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWO45_002265"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWO45_002265"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0333724.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="IWO45_002266"
     CDS             complement(6138..6911)
                     /locus_tag="IWO45_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0333725.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="IWO45_002267"
     CDS             7076..7555
                     /locus_tag="IWO45_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0333726.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="IWO45_002268"
     CDS             7612..7914
                     /locus_tag="IWO45_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0333727.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="IWO45_002269"
     CDS             7936..9393
                     /locus_tag="IWO45_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAQ0333728.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9986..10738)
                     /locus_tag="IWO45_002270"
     CDS             complement(9986..10738)
                     /locus_tag="IWO45_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0333729.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10953..11189)
                     /locus_tag="IWO45_002271"
                     /pseudo
     CDS             complement(10953..11189)
                     /locus_tag="IWO45_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11199..12163)
                     /locus_tag="IWO45_002272"
                     /pseudo
     CDS             complement(11199..12163)
                     /locus_tag="IWO45_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12369..14051)
                     /locus_tag="IWO45_002273"
     CDS             complement(12369..14051)
                     /locus_tag="IWO45_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0333730.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(14062..14385)
                     /locus_tag="IWO45_002274"
     CDS             complement(14062..14385)
                     /locus_tag="IWO45_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0333731.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(14408..15814)
                     /gene="lacG"
                     /locus_tag="IWO45_002275"
     CDS             complement(14408..15814)
                     /gene="lacG"
                     /locus_tag="IWO45_002275"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0333732.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            16281..17288
                     /locus_tag="IWO45_002276"
     CDS             16281..17288
                     /locus_tag="IWO45_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0333733.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17682..>18063)
                     /locus_tag="IWO45_002277"
     CDS             complement(17682..>18063)
                     /locus_tag="IWO45_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAQ0333734.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5431 a         3813 c         2981 g         5838 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc ttttcctcct
     9781 taataaagcg gtgattgata ctgctatttt ttccttcaca taaacataac atcccaaaaa
     9841 aatcgactat cttttttcaa atggtgtatt caagtattcg gaggaattag taatgatgca
     9901 ccctaaaaat aacataattc gcttaaattc aaggctacca aaaaactacc aagttctaat
     9961 tatctagagt ttggcagttt ctcatttaca caacatattt ttcttctttt tcgatttttt
    10021 catagtgctt ttgttcatca tcatccgtta taactactgt tacatcattc agattataat
    10081 atactgaaaa atctcttttc ccaattttcg aatgatcaat caataaaaag cgttcaacag
    10141 aattattcag agcgatctgt tgagtttggc cctcttcaaa agtcgaagtc ataattttct
    10201 gttcatttaa agcattacag ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt
    10261 tattcgtgat ttcaccaaca aagcattgag tcgtttcccg catctcgcca cctaatagat
    10321 agatttttcg attccctttt cgcttattta attcttcaaa aacaggcaaa cagtttgtaa
    10381 caatccgcag tgaatcccct tgaatgattt ttgacatgag ctcgattgta gttcctggtc
    10441 caagaaatat cgtatcacca tcattgatca tttcattcgc cttttccaca atccgctgct
    10501 tttcttcttg cagcataatc tgtttttctc tatgagaaag ctccacatgc cgataaaaat
    10561 ccttcaattt cgctccgcca tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca
    10621 aatcacgtcg gactgtcata tccgaaaccg aaaacttctc cataatatcg ctgactcgta
    10681 ctgtaccgtt aacctctaat aacgacatga tcgcttggaa tcgatccgtt tttaacattt
    10741 catcgcctct tttcttttta agactatcaa attcttaaga aaagtgctag aacaagtttt
    10801 cttaagaagc cttctgcagg aaggaaagca aaatttttca gaatttgcaa gctgctattc
    10861 taaaaaataa aaccgaacga gcatgactgt catagtgaca gccttgttca ttcgactttt
    10921 atctttaata aatcatttta tttaatctga agttattgtg caagttcaaa aacaaagtag
    10981 ttaatgtctc ctcgatattt cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa
    11041 ccctgtgtat ggaaatagaa cattggatct ccttcttcca catctaacaa atcgctgatc
    11101 gtatcatccg ctttgattac ttctagtttt ctggtgaccg ttttaattgg ataacccatt
    11161 tcttcaaaaa cttgatacat tttttctttt ctgatttgtc aaattaagct agacaatatg
    11221 tctttatcta tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt
    11281 ggaatatggt ttcttttcga ggcaacggat tgaataaagg tttcatccat gtccctaaag
    11341 tccattgttt taggtaagcc atctcgtcta agcaagccgt ttgagtgctc gtgctcattt
    11401 agccctcttt gcgaaggtgt tcctggatct gcgaaaaaga tggcaatatc gttcgagtta
    11461 cttatagatc gccagttgga aaattctttt ccacaatcaa atgtcatgga tttaaacaag
    11521 tgtttaggca tggatttgaa ccaatcattt aacctgtttt cgatatcttc ggctcgtctg
    11581 cctgtcggtt tcaaagtaat aataaccttg gataaacact caaccaatgt aataacagcg
    11641 ctcttgtgtt taactcccac aatggtgtcg ccttcgagat gaccaaattc tttagtcagg
    11701 tgtggataga gactgttccg ttcatgaatc gttctgcgaa aggcctgttt tccacgcttt
    11761 tcttgatgat tattcggttt acgtttcccc ttcataggta acgactgtgt gtcaaaaagg
    11821 ccttttttaa acatccgata gagtgtacga acagaacaat caattggaaa ctcgccacgg
    11881 ccgataatca catcgggtga ccacccttga gctacctttt cttgaacata tgttgattgt
    11941 tcatcagata aaacggttga acgtcgtcca caacgagatt tattttgttt gtattgacgg
    12001 taataatcta gcactttttt ccttgtttga gaaacgaata aacattataa atggtttgtc
    12061 tagagcgatt taaactttgt gcgacaagag cgacaggttt ctcgatattg aaataagact
    12121 ctatgattac tagttcgtct gttgtaagat gggtgtaggt catttgtact cactccttat
    12181 taattctttg gtcggaacta ttttgagtgt agcacaaatg atctttttag ttgtctagct
    12241 taattttaca atcgacgcct caaaaagaat acccgaaatt taaacagcaa aaaaagatag
    12301 cgcttaccat tgtgtttaag ataaaacgct agctaagttg gctagcgctt tattaatcta
    12361 agcaagctct aaaattgctt caatacaaaa tcaactgctc ctttaggatc acgtgttaaa
    12421 ttgatatatt ctgcaccttt agttgcaatt acttttatcc ctaatttgtc tgcatcttta
    12481 cggagatcat ccaaatagga attcacttga ggtgctagaa cgattaaatc aaaatttttc
    12541 atcatatcat aatgagaacc ataagctcct gctttagcag aaagattaac acctgtctct
    12601 tttgcgccct cttctaatgc attagccaac attgcactcg ttccggcacc agcacaaagt
    12661 actaaaacgc gctttccttc accaaccgat gcttttgctt catcaactat aactgccgca
    12721 gttgcaccaa cttcttggat ttcttcgacc aattcttctt ttacttcttc tgcagcaatg
    12781 ttttttaact cttgttctac taattcagaa tcataggcct taacaaaagg taaataaata
    12841 atcgcatcga caacaatcaa tactaccgct aatacaatcg ccaacacact aaacccggtt
    12901 cctaaaacca agccaatcgg tgcgggcgtc gcccaaggca atacggatat aaagctattc
    12961 atccccaaga catcgacaaa aagtttgaat atccatacgt tagcgattgg tgctaataaa
    13021 aatggaatta gaaagtacgg attcaaaata ataggagtag caaatagtaa cggttcattt
    13081 accgcaaacg aaactggaat aaatgaagct ttaccaactg ctttcaattg ttttgattta
    13141 gcgaacaaca tgaacagaaa aggaacaact agtgtcgctc cagtaccacc catcgttcca
    13201 acaaagtttc ctagaccaac tgttaatact tttgaggctt gtgcaccgtt tttaaataat
    13261 tcaaagttag tttctacgtt cgcataaata attgcagcaa tcgctggttc aacaatcgat
    13321 ggtccatgaa caccaacaaa ccagaacatt gccatggctc cccaaatgat tgcaataccg
    13381 acatatccat cagcagcact aaataatggc tgcaataatt gaattacacc tgctgcaaat
    13441 gatgttccaa aaaattgacg aactcccgta tcaatcaaaa cggcaataaa tactgcaatt
    13501 gaaaagggga taatatctct aaacatctgt gtgattgttc cgggaacttc ttttggcata
    13561 cggatcgtca aatctctttt aacacagaat ttatacacat ttacagtaac aaacgcagaa
    13621 acgaaagctg ctaataatcc ctttgtccct aaataatcag tagctatccc accatcaatt
    13681 tgatcagctg ccatcattac aaaaccacaa attgacgcta acataacaga aacttcatta
    13741 atctgcttac ctgaatcaga taattgacgg ttcattgaat tagctaaaca tcgagctgtt
    13801 gtagcaacaa ccaaaagacc aactaatccc attgaataac cataaacttt ccaaagccac
    13861 gtcgagaatg cctctggtaa cgtcacgtta aatacttctg gaacagccgc aatcataata
    13921 aaaatacttg aaaatagaat agctggcata gcagttaaaa agccatcgcg aattgcccct
    13981 aaataaatat tacgtgagat tttttcaaaa aacggttttc ccttctcaat ttgcttaata
    14041 atcgtattca tttttcttct cctatgcttt agcaaatttt tctttgtaca attcaacaaa
    14101 atcattgata agttctctta atagaatcgt tgtcattaaa tggtcttgcc catgaataaa
    14161 aattacgcct acttccattt cgttccctga cgcttcttca gctaaacatt tcgtttgtga
    14221 gttatgcgct aaggttaaat tttcatcagc atccgctaat aatcgttcaa cattatcaaa
    14281 attaccttct ctcacttcct tcaacactgt taataatgaa cttctggcat ctcctgcata
    14341 agcaacaatt tcaaatccta ataattgcaa ttctttccta tccatttcct tgccctccta
    14401 actattttta tattatcgtt tttgtttcgc taacaaattt ataccaatat gcagactctt
    14461 tcggatatct tttttgtgta tcgaaatcta catagaatag tccataacgc ttagtgtatc
    14521 cattaaccca agagaaaaca tccattaatg accaaatgaa ataccccttt acattaactc
    14581 ccattttgat agcatcggaa atcgcattaa aatgctggcg taaataatcg attcgatcat
    14641 catctagaat aatcccatct tcaaaattat ccttataccc tagcccgttt tcagtaatgt
    14701 atattttatt ataatttgga tattcgtctt ggattcgtac aagtaaatca tataaccctt
    14761 ttggataaat gatccaatcc caatcggttt tcggaagatc ctcacgcacg attctctcgc
    14821 caatcccttt aatgcaatag acttggctac ctttttctcc ggtagtgttg tagtgaagtg
    14881 tgctttctcc tttgtactct ttaacccaat ggctaccata gttattgata ccaagaaaat
    14941 catttcgatt tgctgctttt ttcatctctc taaaatcatt agaatcaata tctgcagatt
    15001 gcccatttgc tgctaaaatc tcattaatat gtgccattgt ttcatctgag taatacccta
    15061 aataagtagc atctaataag aacttaattg ataacgcatc atctaatttc gcagcatgct
    15121 tatcttcttc tttgtcactt gctggatatt ttgtctctag cgagtgaaca acaccaattt
    15181 cccctttata tccaccatct ttaaaaatgt ttactgtctt tgcgtgcgca tacatcatgt
    15241 tgtgtaaaca agttacagtc ttctctatat caaattgaac ttctggagga aacgatccga
    15301 tcaaatattg atttgaagca actggataaa tctcattaaa tgtactccaa taatgaacct
    15361 cagaaaattc atcgaaacag aactgtgcat agtttacgaa atgttcaaca ttttctcgat
    15421 ttaaaaaatc tccattatca aatagttctt taggggtatc aaaatgatga agagttacaa
    15481 aaggaataat accatgctta ttcaattcag caaatacctt atgataatat tccactcctg
    15541 tttggttcac ttccccatag ccttttggaa aaatacggct ccatgcaata gacatcctaa
    15601 tactatcaat tttaaatttt ttgcataatt cagcatcaac tggatattga ttataaaaat
    15661 cactagctgg atcagcagta tagcgtccct tttctacaag gaaagtatcc cacgctactt
    15721 gcccttttcc accttctttt gtagagcctt ctgcttgata agctgccgta gctcctccca
    15781 taataaagtc ttctggtaaa gttttataat tcattttaat ctctccttga aaattagttt
    15841 gatcaatatg ccaataatcc ataaatcacc tcagcataat tttaacatgt ataagtaaat
    15901 ttttttacac aacgaaaaac cgacctcgaa tatctatttt attgcgatat acaagcactt
    15961 atatcccttg atgtaaaata aatataccat tcaaaaaccg ttttgtaaac gtttttttgt
    16021 aaaataaatt tatcttttta ctaatttaat ttacacgaaa caagaaaacg gactgattcc
    16081 cactaaaatc aatcccacct actaatcatt ctccaagtgg ctatccatta tcgattcaat
    16141 cttctctgtt aatggattag gctctctgct gatatcagag tttcgtttcc tgctagaaaa
    16201 agtaatttaa gttactaaaa gagcagtttc gatttacttt aacaaagaaa aggattatta
    16261 tatccctagg aggatgaaca atggttacta ttagagatat tgcaaaaaaa tctggttact
    16321 caacggctac tatatcaaga ctttttaaag gtgatgagtc actctctatt acatctgata
    16381 ctaaaaacaa aataatcgtt acagccaaat ctttaggata tgatctgaca aaaattaaga
    16441 ctactctgta caagattgcc attctttttt ggatctcaga ggaacagtta aaaaaggatg
    16501 tctactatca acatgtcaaa gatgatttga aaaattatag taaaaaagct aatctagatt
    16561 tgattttcat ctctaaaaac gatggtgtaa aaagcattcc tgacgatatt actggattta
    16621 tcgcaatagg acaattgaca aaaaaggaat tgtactactt aaatcaaaaa gggtataagg
    16681 gggtaatccg aggaataaac ccattacctg aacacttcga tactgtagaa cctgatttcc
    16741 acgaaatggc cagacaagca attaactact ttattaatga aggtttcaca aaaattggat
    16801 ttattggagg aaatttcttt aacctagaaa cgggtaaaga agagattgat agccgtgaat
    16861 atgccttccg atatcattta gaaaagagcg gactactcaa tgaaaagtat attttctccc
    16921 aaggatcatt tactgttgac gaagggtatc aattaactct taaaatgaaa gctgagttaa
    16981 atgaagatat gcccgaggca tgtttcatac catctgacac tatagctgcc ggcgttcttc
    17041 agggctgcca tgaattgggt attaacatcc ctaaagatat ggcagtaata agtattaatg
    17101 atgacgacat cgcaaaattc gttgcaccac cacttacaac ttttaagata gatatcgaag
    17161 agatagctaa aacctgtgta gatttattaa gtgatcaact ggtatattcg cgggacatat
    17221 caaagaaaac attaatcggc gcaaaattga ccgttaggaa aagttttatc cctaaagaag
    17281 aggtgtaaaa taagtaattg aaacttatct ttaaaactat aattgtgtcc acatagtagc
    17341 cgagcaatcg ggtttatgcc tattatgttt tccataaata atttgatggt gatattttga
    17401 cgtaaataca atatgatttt ttcatcgcca aattgtgcgc gctaattttt gtttatcatt
    17461 cagaacattt cctccttttt atctaagtat gaggttggat aaacgcttca tcttagtatc
    17521 ttgggggatt ttttatctcg ttgggaatat atcaagcatc agagcgcgca aaaagaactg
    17581 gtcgaaaaat caatctacca gttcaatctc attcactgtg atactatgtt ttatacagat
    17641 aacgccatat tataaaacaa gttgtattcc gcaaatgggt tctattcaac tcttaaacta
    17701 tctataaaag attcagccaa ctccattagc ctctcgtaat ttagttggtg aacagcactt
    17761 ttttcaaaaa attttgaacc aattcctaca taagaggctc cattgttcaa atagttttgt
    17821 gcattagtct gatctacccc tcctacagcc atcaaaggta aacttcctaa aggggcttgc
    17881 acttgattga aatattccgg tccaagacag ttggcgggga acaccttaac aatatcagca
    17941 cccatctcaa acattttgaa tatctctgtg ggcgacatgg ccccaggaat tgttagaagg
    18001 ttctgttgcg aagtttgaaa atcaaacgcg ccctctctga actccaaata tcttaggctg
    18061 tta
//
LOCUS       DACEDD010000060        17868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEDD010000060 DACEDD010000000
VERSION     DACEDD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.060"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..325
                     /gene="sgrA"
                     /locus_tag="IWO45_002278"
     CDS             <1..325
                     /gene="sgrA"
                     /locus_tag="IWO45_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0333735.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            623..979
                     /locus_tag="IWO45_002279"
     CDS             623..979
                     /locus_tag="IWO45_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333736.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            982..2139
                     /locus_tag="IWO45_002280"
     CDS             982..2139
                     /locus_tag="IWO45_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333737.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2165..2605)
                     /locus_tag="IWO45_002281"
     CDS             complement(2165..2605)
                     /locus_tag="IWO45_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0333738.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2765..3271
                     /locus_tag="IWO45_002282"
     CDS             2765..3271
                     /locus_tag="IWO45_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0333739.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3439..5352
                     /locus_tag="IWO45_002283"
     CDS             3439..5352
                     /locus_tag="IWO45_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0333740.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5357..6358
                     /locus_tag="IWO45_002284"
     CDS             5357..6358
                     /locus_tag="IWO45_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0333741.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHFDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="IWO45_002285"
     CDS             complement(6560..6766)
                     /locus_tag="IWO45_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0333742.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(7412..8260)
                     /locus_tag="IWO45_002286"
     CDS             complement(7412..8260)
                     /locus_tag="IWO45_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0333743.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            8424..9326
                     /gene="gnd"
                     /locus_tag="IWO45_002287"
     CDS             8424..9326
                     /gene="gnd"
                     /locus_tag="IWO45_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0333744.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9328..10329
                     /locus_tag="IWO45_002288"
     CDS             9328..10329
                     /locus_tag="IWO45_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0333745.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10326..10610
                     /locus_tag="IWO45_002289"
     CDS             10326..10610
                     /locus_tag="IWO45_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0333746.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            10643..11440
                     /locus_tag="IWO45_002290"
     CDS             10643..11440
                     /locus_tag="IWO45_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0333747.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            11454..12287
                     /locus_tag="IWO45_002291"
     CDS             11454..12287
                     /locus_tag="IWO45_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0333748.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12305..13162
                     /locus_tag="IWO45_002292"
     CDS             12305..13162
                     /locus_tag="IWO45_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0333749.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13184..13693
                     /locus_tag="IWO45_002293"
     CDS             13184..13693
                     /locus_tag="IWO45_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0333750.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            13730..14131
                     /locus_tag="IWO45_002294"
     CDS             13730..14131
                     /locus_tag="IWO45_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333751.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14243..14596
                     /locus_tag="IWO45_002295"
     CDS             14243..14596
                     /locus_tag="IWO45_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333752.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            14630..15073
                     /locus_tag="IWO45_002296"
     CDS             14630..15073
                     /locus_tag="IWO45_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0333753.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15090..16175
                     /gene="uxuA"
                     /locus_tag="IWO45_002297"
     CDS             15090..16175
                     /gene="uxuA"
                     /locus_tag="IWO45_002297"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0333754.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16168..16881
                     /locus_tag="IWO45_002298"
     CDS             16168..16881
                     /locus_tag="IWO45_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0333755.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            17633..17833
                     /locus_tag="IWO45_002299"
     CDS             17633..17833
                     /locus_tag="IWO45_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0333756.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         6241 a         2767 c         3728 g         5132 t
ORIGIN      
        1 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
       61 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
      121 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
      181 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
      241 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
      301 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
      361 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
      421 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
      481 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
      541 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
      601 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
      661 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
      721 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
      781 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
      841 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
      901 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
      961 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     1021 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     1081 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     1141 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     1201 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     1261 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     1321 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     1381 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     1441 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     1501 agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt
     1561 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     1621 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     1681 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     1741 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     1801 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     1861 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     1921 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     1981 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     2041 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     2101 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     2161 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     2221 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     2281 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     2341 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     2401 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     2461 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     2521 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     2581 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     2641 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     2701 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     2761 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     2821 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     2881 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     2941 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     3001 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     3061 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     3121 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     3181 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     3241 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     3301 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     3361 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     3421 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     3481 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     3541 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     3601 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     3661 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     3721 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     3781 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     3841 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     3901 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     3961 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     4021 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     4081 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     4141 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     4201 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     4261 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     4321 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     4381 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     4441 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     4501 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     4561 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     4621 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     4681 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     4741 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     4801 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     4861 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     4921 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     4981 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     5041 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     5101 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     5161 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     5221 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 ttttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
     6841 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
     6901 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
     6961 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
     7021 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
     7081 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
     7141 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
     7201 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     7261 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     7321 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     7381 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     7441 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     7501 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     7561 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     7621 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     7681 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     7741 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     7801 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     7861 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     7921 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     7981 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     8041 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     8101 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     8161 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     8221 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     8281 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     8341 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     8401 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     8461 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     8521 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     8581 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     8641 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     8701 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     8761 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     8821 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     8881 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     8941 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     9001 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     9061 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     9121 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     9181 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     9241 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     9301 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     9361 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     9421 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     9481 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     9541 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     9601 gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta
     9661 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     9721 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     9781 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     9841 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     9901 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     9961 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    10021 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    10081 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    10141 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    10201 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    10261 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    10321 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    10381 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    10441 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    10501 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    10561 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    10621 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    10681 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    10741 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    10801 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    10861 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    10921 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    10981 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    11041 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    11101 aaatgtggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    11161 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    11221 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    11281 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    11341 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    11401 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    11461 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    11521 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    11581 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    11641 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    11701 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    11761 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    11821 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    11881 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    11941 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    12001 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    12061 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    12121 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    12181 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    12241 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    12301 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    12361 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    12421 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    12481 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    12541 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    12601 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    12661 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    12721 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    12781 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    12841 cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga
    12901 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    12961 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    13021 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    13081 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    13141 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    13201 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    13261 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    13321 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    13381 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    13441 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    13501 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    13561 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    13621 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    13681 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    13741 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    13801 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    13861 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    13921 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    13981 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    14041 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    14101 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    14161 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    14221 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    14281 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    14341 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    14401 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    14461 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    14521 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    14581 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    14641 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    14701 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    14761 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    14821 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    14881 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    14941 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    15001 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    15061 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    15121 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    15181 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    15241 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    15301 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    15361 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    15421 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    15481 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    15541 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    15601 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    15661 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    15721 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    15781 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    15841 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    15901 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    15961 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    16021 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    16081 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    16141 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    16201 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    16261 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    16321 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    16381 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    16441 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    16501 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    16561 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    16621 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    16681 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    16741 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    16801 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    16861 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    16921 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    16981 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    17041 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
    17101 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
    17161 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
    17221 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
    17281 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
    17341 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
    17401 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
    17461 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
    17521 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
    17581 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
    17641 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
    17701 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
    17761 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
    17821 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaa
//
LOCUS       DACEDD010000061        17530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEDD010000061 DACEDD010000000
VERSION     DACEDD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.061"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            282..521
                     /locus_tag="IWO45_002300"
     CDS             282..521
                     /locus_tag="IWO45_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333757.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IWO45_002301"
     CDS             complement(883..1143)
                     /locus_tag="IWO45_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333758.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IWO45_002302"
     CDS             complement(1328..1825)
                     /locus_tag="IWO45_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0333759.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IWO45_002303"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IWO45_002303"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0333760.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IWO45_002304"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IWO45_002304"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0333761.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="IWO45_002305"
     CDS             complement(4547..5209)
                     /locus_tag="IWO45_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0333762.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="IWO45_002306"
     CDS             complement(5219..6034)
                     /locus_tag="IWO45_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0333763.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="IWO45_002307"
     CDS             complement(6296..6739)
                     /locus_tag="IWO45_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0333764.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6770..6883)
                     /locus_tag="IWO45_002308"
     CDS             complement(6770..6883)
                     /locus_tag="IWO45_002308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0333765.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(6873..7211)
                     /locus_tag="IWO45_002309"
     CDS             complement(6873..7211)
                     /locus_tag="IWO45_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0333766.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="IWO45_002310"
     CDS             complement(7199..7576)
                     /locus_tag="IWO45_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0333767.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="IWO45_002311"
     CDS             7800..8993
                     /locus_tag="IWO45_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0333768.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="IWO45_002312"
     CDS             complement(9159..9581)
                     /locus_tag="IWO45_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0333769.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="IWO45_002313"
     CDS             9976..10170
                     /locus_tag="IWO45_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333770.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10661
                     /locus_tag="IWO45_002314"
     CDS             10167..10661
                     /locus_tag="IWO45_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333771.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10823..11995)
                     /locus_tag="IWO45_002315"
     CDS             complement(10823..11995)
                     /locus_tag="IWO45_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0333772.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12201..13016)
                     /locus_tag="IWO45_002316"
     CDS             complement(12201..13016)
                     /locus_tag="IWO45_002316"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0333773.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13166..13612)
                     /locus_tag="IWO45_002317"
     CDS             complement(13166..13612)
                     /locus_tag="IWO45_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0333774.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13609..14613)
                     /locus_tag="IWO45_002318"
     CDS             complement(13609..14613)
                     /locus_tag="IWO45_002318"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0333775.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14653..14982)
                     /locus_tag="IWO45_002319"
     CDS             complement(14653..14982)
                     /locus_tag="IWO45_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0333776.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15108..17435)
                     /locus_tag="IWO45_002320"
     CDS             complement(15108..17435)
                     /locus_tag="IWO45_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0333777.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         5066 a         3479 c         3052 g         5933 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgcga cagcaatcag cagctgaaca ggcctctcag caggcacaag
    10501 ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat
    10561 accatacaca taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag
    10621 ctcgtggttt aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa
    10681 taataaaatt cttctgaaac ataaaaaaat agaagatggc gttccagata gtaaaccaaa
    10741 aataacagtt tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct
    10801 ctgctttttt ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt
    10861 ttcgcagttt atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt
    10921 ttacatggat tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat
    10981 cagaaaatct cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag
    11041 taatatagtg tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc
    11101 cgtttgtgtt ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat
    11161 ctaaataagc gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc
    11221 aaataaaaaa tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt
    11281 ttgcttgata aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag
    11341 gagtacaggt aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt
    11401 catcaggtaa gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct
    11461 ggatgaaaaa ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg
    11521 ggagtccacg atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac
    11581 tttcttccaa taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag
    11641 gtaagctgac attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg
    11701 cattactagg taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc
    11761 ctggtgatac gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt
    11821 tttttatttc ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct
    11881 gggcaagaaa atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa
    11941 cgactaaaat gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt
    12001 ccacctattt tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat
    12061 tagcgaagca aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag
    12121 agaccgacca cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta
    12181 tgatttttgt ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc
    12241 actgctcctc caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca
    12301 gttgaaggca agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga
    12361 tcatcatcta ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct
    12421 ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc
    12481 ccttctgaat cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac
    12541 caagcatatg ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt
    12601 ggatcttttg ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca
    12661 tcttgtttga tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta
    12721 tccgtagttg tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct
    12781 gcgccatgga tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt
    12841 tctttttgtg gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa
    12901 ttgccgtcta ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac
    12961 gctgactcag caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat
    13021 tgattctctg aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag
    13081 ttcccagctt tctggatttt gatattgctt tctttgatga catttttagc atataggtga
    13141 atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt
    13201 cgacaccggg ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac
    13261 ctggaagagt caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag
    13321 cgtctgctgg aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt
    13381 tgaattcaac gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta
    13441 tttgattttc tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg
    13501 gtgtaggtga tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca
    13561 gcatgatagc agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc
    13621 cctcctattg tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac
    13681 tagcaataac cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct
    13741 tcctccattt ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt
    13801 attcgtcgga tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc
    13861 ataactgcct ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg
    13921 cggtttaatc tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc
    13981 atctttactg gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat
    14041 cccgttttct tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag
    14101 tttttgtttc gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag
    14161 atgtgtttct actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt
    14221 ttcactgttt tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga
    14281 actaagatct tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa
    14341 tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg
    14401 aggatcagct gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt
    14461 tacggtgatg agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac
    14521 tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat
    14581 ggtcgtcaat ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga
    14641 tttttctttt acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc
    14701 ttgctcatct ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt
    14761 tgtcacattt tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc
    14821 gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat
    14881 agcgaaagta acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc
    14941 gtcgttttga ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa
    15001 tgtaataggt gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat
    15061 aaaaaagaga acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca
    15121 cctgcttttt tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct
    15181 aaaaagacaa agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg
    15241 atcgtcaact tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg
    15301 cctaaactaa tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta
    15361 taatccaatg attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag
    15421 tgactttggg catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta
    15481 tagatggtat aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct
    15541 gctttgactt tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc
    15601 atattttttg ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact
    15661 ggagtaagcg aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct
    15721 tttccatcat atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta
    15781 tagacatcag atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct
    15841 agtccgcttt ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact
    15901 tcaactccct tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata
    15961 ctgagatcat tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg
    16021 atcggtacaa cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt
    16081 gcatttgtct tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg
    16141 attacatttc catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt
    16201 tctatcgctg aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg
    16261 tcttgtgcag cacttccgta agtaagattc attattttag aaggagcaat catctgtcca
    16321 aaatagtctt gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg
    16381 acgttacttt ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact
    16441 gctgttggag tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac
    16501 gttccgattt ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca
    16561 ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca
    16621 acgatctgcc catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata
    16681 ccgattgaaa aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta
    16741 aaagaataac ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc
    16801 acccccatat agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata
    16861 aacagattat ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt
    16921 aacgcagttt tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca
    16981 tatatcactg tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca
    17041 gtactaaatg tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct
    17101 gctgtgattt ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc
    17161 tcgttgttgc tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta
    17221 tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa
    17281 atacttgata agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt
    17341 aagtattttc ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt
    17401 tggattttat tttgagtttt cttaatcatt ttcatccctc ctctcaataa aaaatcatca
    17461 aataatcctt tacacgttat tctaaattta atttttatta ttttattgat gtatagaaag
    17521 tttggggctt
//
LOCUS       DACEDD010000062        17469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEDD010000062 DACEDD010000000
VERSION     DACEDD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.062"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            363..731
                     /locus_tag="IWO45_002321"
     CDS             363..731
                     /locus_tag="IWO45_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0333778.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IWO45_002322"
     CDS             911..1852
                     /locus_tag="IWO45_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0333779.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IWO45_002323"
     CDS             1921..2652
                     /locus_tag="IWO45_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0333780.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IWO45_002324"
     CDS             2796..4976
                     /locus_tag="IWO45_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0333781.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IWO45_002325"
     CDS             4996..5478
                     /locus_tag="IWO45_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0333782.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IWO45_002326"
     CDS             5494..6255
                     /locus_tag="IWO45_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0333783.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IWO45_002327"
     CDS             6386..7720
                     /locus_tag="IWO45_002327"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0333784.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IWO45_002328"
     CDS             8027..8512
                     /locus_tag="IWO45_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0333785.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IWO45_002329"
     CDS             8505..9188
                     /locus_tag="IWO45_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0333786.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IWO45_002330"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IWO45_002330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0333787.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IWO45_002331"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IWO45_002331"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0333788.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IWO45_002332"
     CDS             12804..13433
                     /locus_tag="IWO45_002332"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0333789.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IWO45_002333"
     CDS             13485..13817
                     /locus_tag="IWO45_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0333790.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IWO45_002334"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IWO45_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0333791.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IWO45_002335"
     CDS             14643..16016
                     /locus_tag="IWO45_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0333792.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IWO45_002336"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IWO45_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0333793.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..>17469
                     /locus_tag="IWO45_002337"
     CDS             17071..>17469
                     /locus_tag="IWO45_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333794.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQ"
BASE COUNT         5948 a         3058 c         3845 g         4618 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaa
//
LOCUS       DACEDD010000063        16687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEDD010000063 DACEDD010000000
VERSION     DACEDD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.063"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..571
                     /locus_tag="IWO45_002338"
     CDS             <1..571
                     /locus_tag="IWO45_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0333795.1"
                     /translation="NGIDADFVSPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALG
                     NTLRPDDVDANDYAAVYYAGGHGTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGL
                     LPVTDESGKSILAGRTVTGFSNEEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEV
                     VQIDGRLLTGQNPQSAHQLGEAVVEALKN"
     gene            903..2198
                     /locus_tag="IWO45_002339"
     CDS             903..2198
                     /locus_tag="IWO45_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0333796.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            2188..3648
                     /locus_tag="IWO45_002340"
     CDS             2188..3648
                     /locus_tag="IWO45_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0333797.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            3784..5280
                     /gene="glpK"
                     /locus_tag="IWO45_002341"
     CDS             3784..5280
                     /gene="glpK"
                     /locus_tag="IWO45_002341"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0333798.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            5294..7117
                     /gene="glpO"
                     /locus_tag="IWO45_002342"
     CDS             5294..7117
                     /gene="glpO"
                     /locus_tag="IWO45_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0333799.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            7118..7834
                     /locus_tag="IWO45_002343"
     CDS             7118..7834
                     /locus_tag="IWO45_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0333800.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            8140..10068
                     /locus_tag="IWO45_002344"
     CDS             8140..10068
                     /locus_tag="IWO45_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0333801.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            10194..10748
                     /locus_tag="IWO45_002345"
     CDS             10194..10748
                     /locus_tag="IWO45_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0333802.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11233..11547)
                     /locus_tag="IWO45_002346"
     CDS             complement(11233..11547)
                     /locus_tag="IWO45_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0333803.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11561..11821)
                     /locus_tag="IWO45_002347"
     CDS             complement(11561..11821)
                     /locus_tag="IWO45_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0333804.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11991..12305
                     /locus_tag="IWO45_002348"
     CDS             11991..12305
                     /locus_tag="IWO45_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0333805.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            12307..13962
                     /locus_tag="IWO45_002349"
     CDS             12307..13962
                     /locus_tag="IWO45_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0333806.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            14045..16180
                     /locus_tag="IWO45_002350"
     CDS             14045..16180
                     /locus_tag="IWO45_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0333807.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            16355..>16687
                     /locus_tag="IWO45_002351"
     CDS             16355..>16687
                     /locus_tag="IWO45_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333808.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         5587 a         2824 c         3657 g         4619 t
ORIGIN      
        1 aaatgggatc gatgcagatt ttgtcagtcc taaaggcgga tatgtacctt tagaaccgca
       61 aagtgttttg aatatggatg aaatcgattg ggcctattat gaagatgaag atttcagaaa
      121 tcgggccttg ggaaatacct tacgtcctga tgatgtggat gcaaatgatt atgcagcggt
      181 ttattatgct ggtggccacg gaacaatgtg ggattttcca gaagcaatag atcttgggaa
      241 agtcgcttca caaatctatc gaaatgaagg gctggtctca gctgtttgtc acggtgttgt
      301 aggattgttg ccagttacag atgagtctgg aaaatctatt ttagctggac gtacagtgac
      361 tgggttcagt aatgaagaag aggaagcaaa tggtacaacg aatgaagtac cattcttagc
      421 agaagatgct ttgaaagaaa aaggagcaaa ctatcagtct ggagcagctt tttcagaagt
      481 ggttcaaatt gatgggcggc ttcttacagg gcaaaatcct caatctgctc atcaattagg
      541 cgaagcagtt gttgaagcat taaagaatta aaaataaatg atgtgtctat tctaactatt
      601 acattacaca cgctaaaact cgtcttttcg ttcttttctg tttcctattt ggacagaggt
      661 acgtaaaagg cgagtttttt tcttttataa gagtacattt tttgaaacat ctcttcagaa
      721 atgatactct tcaattaggt ccactagtac attattgaga agcagaccta gaacacggta
      781 tgatggagag ggttgattct gctgcatcgc tatgtatcat aaggttgaaa gcgagttatg
      841 aatactgtgt tttctgattg agaagttagc agtagaacac cttataagaa agaggaatgc
      901 agatgaacat tgtaattgtc ggcgggtcgt ttgcagggat tcatgcggct atttatcttc
      961 gccaattgat gccaacaagt gaaatatatt tgattgaaaa gcaatcgaag ttaggatgga
     1021 ttcccagcgg gttcaatttg atattaaaag gaaaggttac cagtgaaaaa caactttctt
     1081 ggatcacgaa agaagaactg actgaccgct atcgaatcca tgtttacaca gaaaagacag
     1141 taataggatt gaaagaaaaa aaagtcgttt tagcaggagg aaaaaaattg gattttgatc
     1201 gtttgattct agcgactggt tcaaatcaaa agtttcgaaa tatttctgca gaatcaacgc
     1261 ttattcaccc agttaaaaag atacaaaacg taacggctct tcagcaaaag atcatgaatg
     1321 caaagaaaat tgcaattatt ggtgcaggac aagcaggaat cgaaatagct gaagggttgg
     1381 catcaagaaa aaaacaaatc catctttacg aaagccgaaa gtcaattctt tttcgttatc
     1441 tagatcctga aatgacagaa ccgttagtaa aggaaatgaa aaatcaaggg ctttcacttt
     1501 ttttagaaga acaagttgtg tctttgaccg aaaatgaaac cgctattgta gagacggaaa
     1561 aaagaaagga gaactatgat cttgttttgc tggctaatca ttctcgcccc gacaatcaga
     1621 tgtgggaaga acaattaaca ttgaatgatg atggaacgat ctgggtagat gactacttgc
     1681 aaacgtctca aaaagacgtt tatgcgattg gcgatgcgat ccaagtaact tttcgtccaa
     1741 caaatgaaaa aatgtatgtt tctctagtaa ataacgctgt ccgtacagca cgaaatgtca
     1801 gcaagacaat tagtggctca cagacaaagg atcagggaac ctatcgtcca gtcggcaatc
     1861 agtggtttgg ctatttctta ggaagcgtag gtttgacaga agaagagagc attttttatc
     1921 cccaaaaaat tgaaacagga tacgtagact acaagcttac agcaataaat caaagcaaag
     1981 ttaaactcaa atttctattt gattctgaaa aaaagattct tggagcacag ctccaaagca
     2041 aaaaagagat gttccacctt cttgaccaac tgacaattgc agtaaaagaa ggatggacgt
     2101 ttgaacagtt agaagaacat gagctatttt tccagccaga ataccgtgtg ccagatatgg
     2161 tgtggtcaaa ggtggcgaat actgcctatg aagattgaag cattattaga aagaaaagag
     2221 cagttacaga tcctgatttt gcgaaacctt gttctgcaag ggggaacagc ctctatcaat
     2281 ggtttgagag agcatgtcca actgtcaaaa gcctcgtttg atcaatattt ggaagatatt
     2341 gaattgattg gccgaatgat ggaaaaaaaa gtagaagtac agcgaaatga gtatcaggcg
     2401 ctgcttgtgt tggacgaaga tgtaagtttg gaaaaaattc tattgttttt attgcaggaa
     2461 agcttgaagt ttaagatgct cgtctattta ttagaacatc agcaagtatc catcgttcga
     2521 ttagcgacag cgtttaatat cagtgagtct tctgtttttc gaaagatcaa agaattgaac
     2581 cagctcttag aggaatttgg attacaaatc aaaaacggtc aattatacgg agaagagcta
     2641 cagatacgat atttttatta tgaactattc caatatatcc cggaagatca gcgaccgctt
     2701 tttttgcaaa atacaccaga aaaaagaccg ttcattctag gattggaccg tgtattagaa
     2761 acaacgttta cggcatcagc agaagcacag attgcttgct ggctcgggat tacgaaaaaa
     2821 agactattga atgaaaaaag tacgtacgca actttgaaag aaaagaaatt actctatcag
     2881 tcggaccggc tttatcaagc aatcgatcca atcatcgtga tgcatcttag tcgaacagca
     2941 gcagaactga atgtatacga aacaatgatg ttttacagct tttttgtttc tttttcgata
     3001 gtagacgaag aaatatttta tcagtatgat ttgacacgga gcaaaaagtt accaaccgct
     3061 gttttagata cttacattcg ggaaacgatg ttatggcatt atcgtcctcg tcgtttgaaa
     3121 atcaaagaag aaaaggcagt aggctaccaa atttcccaga tcaacaatga atggtttttc
     3181 tttgttggaa agatcgaagt ctatgagcgt gatcggcttt tggaacagca gcagaaaatg
     3241 ctcgggcatt ccttaacaca gttactggcg aaattacagg aaacagcggt gcagcaattg
     3301 ccgaggaaaa gagctgaaga tagtgagctt agttacctga tgatccagta tgccaatgtt
     3361 cttttgatga tcgattttta tattgcaaaa ccagtagtga ttggaatcga tttagaaagt
     3421 ctgccgattt atcgtattgc ttttcaacaa tatctgatca gagaactgag gggaatcggc
     3481 ggtattgaga ttggaagtta tgaagaaggt aaagaatatg atttggtgat cacattttgc
     3541 cagagaaaca aaaaacagtg tgaatattat ctgtcagaat ttgcgtcacc ttacgacatt
     3601 attcgattga aaagaagaat agagacgctg aaaaaagaaa agaattgatt aaaaaatagc
     3661 ttcataataa aaaactgtca aaaaaagaaa aaaagaataa aattttgaca gtttcttttt
     3721 ttaaaacagt agtatgataa gagtagttaa agaaagcgtt tgcatataat ggagggaatc
     3781 acaatgacag aatcaacgta tatcatggcg attgaccaag gtacaactag ctcacgagca
     3841 attatctttg acaagaaagg aagacacatc ggcagttctc aaaaagagtt cacgcaatat
     3901 ttcccgcaag aaagttgggt ggaacatgat gcaaatgaaa tctggaattc tgtacagtca
     3961 gttattgccg gagcgttcat cgaatcaggg atcaaaccaa atcagattgc tgggattggc
     4021 ataacgaacc agcgtgaaac aacagtaata tgggaaaaag atacaggacg tccaatctat
     4081 catgcagttg tttggcaatc cagacagtca gctggaattg cagataactt aaaaaatgaa
     4141 ggctatcaag aatttttcca cgaaaaaaca gggttagtga tcgatgccta tttctcagca
     4201 acgaagatac gctggattct tgatcatgta gaaggcgcgc aagaacgagc tgaaaaaggc
     4261 gaattgatgt ttggaacgat cgattcttgg ttagtatgga aactgaccga tggacaagca
     4321 catgttactg attactcgaa tgccagtcgg acgatgttgt tcaatatcca tcagctagat
     4381 tgggatcagg agattttaga tttactaaat attcctcgag tgatgttacc aaaagtcact
     4441 tctaactcag aagtttatgg ttatacgcaa ggttatcatt tttatggaag cgaagcacca
     4501 atttcaggga tggcaggaga ccagcaagcc gcattattcg gacaaatggc attcgaacca
     4561 ggcatggtca agaatactta tggtacaggt tcatttattg tcatgaatac cggtgaaaaa
     4621 ccgcaattat ccaaaaataa tttattgacg acaattggtt acggcataaa tggaaaggtt
     4681 tactatgctt tagaaggaag tatttttgtg gctggttctt cgattcaatg gctgagagat
     4741 ggacttcaaa tgttgcaaaa agctagcgat tcagaagacg ccgcgaaacg ctctacaagc
     4801 gaagatgagg tttatgttgt tcctgcattc gttgggctag gtgctcctta ttgggatcag
     4861 gcagcacgag gggctatgtt tggcttgaca cgtggtacga caaaagaaga tatcattaaa
     4921 gctactttac agtcgattgc ttatcaagtg agagatatca tcgatacgat gcaagatgac
     4981 actggaatca agattcctgt attgaaagta gatggcggtg cagcaaacaa tgaatatctg
     5041 atgcaattcc aaacagatat cttgaatgta ccgatccaac gagcagaaaa cttggaaaca
     5101 acggcgctag gggcagcatt tttagcagga ttagctgtcg gttattggaa agatacagac
     5161 gagatccgcg aattttatga agctggcaag ctatttgaag ttcaaatgga ggaagagcgc
     5221 cgagaaaaac tttacaacgg ttggaaaaaa gcagtaaaag ctacccaagc atttgaataa
     5281 ggaggaaatc catatgtttt caaacaagac aagacaagat agcattcaaa aaatgcagca
     5341 agaagaattg gatctgttga tcatcggtgg cggaatcact ggtgccggtg tggcagtcca
     5401 gtcagcagca tcaggaatca aaacaggatt gatcgaaatg caggattttg cagaagggac
     5461 ttcctctcgc tcgaccaaac ttgttcatgg cggtattcgt tatctgaaga catttgatgt
     5521 ggaagtagta gctgacacag ttggtgaacg tgcagtcgta caaagtattg ccccacacat
     5581 cccaaaacca gatccaatgc ttttgccaat ctatgagggc gaaggagcaa caaccttcaa
     5641 tatgttctcg gtcaaagtag caatggacct ttatgacaaa ctggcaaatg tgacaggaac
     5701 caaatatgag aattatacgc tgacaccaga agaagtattg gaaagagaac cgtttttgaa
     5761 aaaagaaggg ctaaaaggcg cgggtgtcta tctagatttc cgcaacaatg atgcccgttt
     5821 agtgattgat aatatcaaaa aggctgccga agatggggct tatctagtaa gtaaaatgaa
     5881 agcggttggc tttttatatg atggcgatca aatcgttggc gtcaaagctc gtgacctgct
     5941 gacagatgaa gtgatcgaaa tcaaagcaaa attagtgatc aatacgagtg gtccttgggt
     6001 agataaagta cgaaacttga attttacgcg tccagtctct cctaaaatgc gtccaactaa
     6061 agggatccat ttagttgtag atgcgaaaaa actgcctgta ccgcaaccca catacttcga
     6121 tacaggaaag caagatgggc ggatggtttt tgctattcca agagaaaaca agacttactt
     6181 tggtacgaca gatacggatt accaaggaga ttttacggat ccgaaagtca cacaagaaga
     6241 tgtagattat ctattggatg tgatcaatca tcgctatcca gaagcaaata tcacattgaa
     6301 tgatatcgaa gcaagctggg caggacttcg tccactgttg attggtaatt ctggttctga
     6361 ttacaatggt ggagataatg gatcgatttc agacaagagc ttcaataaag ttgtcgatac
     6421 agtaagtgaa tataaggaaa acaaagtttc tcgtgctgaa gtagaagacg tgttgaacca
     6481 tttggaaaac agccgtgatg aaaaagcacc ttctacgatt tccagaggta gttctttaga
     6541 aagagaacca gatggtttgc tgactttatc tggtgggaaa atcaccgatt accgtaagat
     6601 ggcagaagga gctttgcgat tgatacgcca attattaaaa gaagaatacg gaatagagac
     6661 gaaagaaatc gactctaaaa aatatcagat ttcaggtgga aacttcgatc caactaaatt
     6721 agaagaaaca gtgacagaat tagcaaaaga aggagtagca gccggtttag aggaagaaga
     6781 tgctacttat atcgctgatt tttacgggac taatgcccga cgtatctttg aattagcaaa
     6841 agaaatggca ccttatcctg gtttgagtct cgctgagtca gctcggttac gttatggttt
     6901 agaagaagaa atggttttgg ccccaggcga ttatctcatt cgtcgtacaa atcatctgtt
     6961 gtttgaacga gatcagcttg atgagatcaa gcaacctgtg atcgatgcaa ttgctggata
     7021 ttttgaatgg acagaagagg agaaggcgca acagactaaa cgtttagaag cattgattgc
     7081 agaatcagat ctgcgggaac taaaggggga gaaataaatg aacagtgata tgactcaaat
     7141 catgggggag tttattggga cactgatcct agttttgctt ggagatggtg tgtgtgcggc
     7201 tgtgaattta aacaaaagta aagcacaggc ttctggctgg attgtcatcg catttggttg
     7261 gggactcgct gtaacaatgg ctgtctatat ttctggattc atgggcccag ctcatttgaa
     7321 tcctgcagtc tctctagcta tggcgatgac aggggcaatt agttggaatc ttgtcgttcc
     7381 tttcatcatt gcgcaagttt taggagcttt tgcaggggcc attcttgttt ggctttctta
     7441 tttgccacat tggaatgcaa cgaaagatga aagcgcgatt ttaggaacgt ttgcaacagg
     7501 tcctgctata cggaattatc cagcaaatgt gatcacagaa ttaattggta catttgtatt
     7561 agttcttggg ttgctagctt ttggacaaaa cgaatttgca cctggaacga acgtattcgc
     7621 tgtaggtgga ttgatcttag ccatcggtct ttcattagga gggccaacag gatatgcaat
     7681 caaccctgct cgggactttg gtcctcgatt agcacatgcg gtattgccaa tagctaataa
     7741 aggaacttca gattgggcat attcatgggt accaatcgca ggtccaatga tcggagcaat
     7801 tatcgcggta ggtgtctatt cattaatggt gtaaatctat aaaacatact aaagtttgac
     7861 aaccaaaatg tgtcttgcta tagttaagtg gaaaataaat aagacctaga agaatggggt
     7921 atgttttgtg agtgtagctg gtatctaatc agtaagtcga atagccaata aacaaagttt
     7981 atttggtgtg cttatgaaac ggtacctgtc aggaaaacaa tatctttgta ttctaataag
     8041 gataggacat gactttttta agctgtgttc tatcctttta tttatctcta ggcccttatt
     8101 tattttggga caggtatctc ttcaagaaaa ggagagaata tggacaacga aatgattagt
     8161 caattacaat ttgatcggat caaaaaggaa atccaagcaa gagcaattgg aaactatagc
     8221 aaaaaacgaa tcagcgatat gacggtctcg acaaacctac aaactgtctt ggatcgtcaa
     8281 gaagaaacaa gagaagcacg cttgatctta gaaagcagtc agcatgttcc atttatgggg
     8341 ttgccgagga tcgatgcact gacagaacaa gtcaaaaaag ggttgattct gcagccaacc
     8401 gatttgatcg aatatgcgga ttttcttaga agcagtcgga tgatcacaaa gttttttgat
     8461 aagaatcaat accagacacc cttgttgttt gcttatagca aacatcttcc tgatttgata
     8521 aatgtagaag agttgattga ccagaaaata aaaaataata aagtcagtga cgacgcgtca
     8581 cgtaatttgc gtaaagtacg caaacaatta caactaatcg aaaaagaaat ccagtcaaaa
     8641 ttactgaaat tcttgcgtca tccgaaaaac aaagaaatga tccaagaagc aatgattgtt
     8701 caaaaaggcg aatactatac gattcctatc aaagccagct ataaaaataa aatagatgga
     8761 acgattatcg atgaatcaaa taaaagaacg acggttttta ttgaaccaac tgtgatttct
     8821 aaattgaatg aacactatca gttgttaaaa gctgaagaaa tcagtgaaga atatcaagtc
     8881 ctggcggcat tgactggtgc tatcgcagaa aacgaagaag tgatcgattt attgatcgaa
     8941 acgatcacgg tgttagatat catctttgct cgggcaaaat ttagccgaga aatcaatgga
     9001 agcacaccta gaatcaataa atcggaacat atcattatca agcaaggcag acatccattt
     9061 ttacctgagc atgcggtacc attggatgtt gaaatcggta aagactatcg aggcttgatt
     9121 atcaccggag caaatgccgg aggcaaaaca gtcgtgttga agacagtcgg tttattgact
     9181 ttgatggcga tgttcggtat gcaagttccg gcaaaagaag ggacagagct tgctgtcttt
     9241 gatgagattt tcgtcgatgt cggtgaccac caaaatttag aaaatgcgtt gagtacattt
     9301 tctggtcata tgcaaaacat cgctgctatt ttgaaaaaga taaaaaggaa tacgttggta
     9361 cttttagatg agattggcag tggaacagag ccaaatgaag gggctgcact agcgatcgca
     9421 atcatggaat ctatgtatga gcaaggggca ttgatcattg ccacgaccca ttatggagaa
     9481 atcaagaaat tcgccggaga tcatgaagat tttgtgccgg ctgctatggc tttcgatcgg
     9541 gaagcacttc gtcctaaata tcagttgcgg gttggagaaa caggagaaag ccaagctttg
     9601 tggattgctc ataaaatgga tatgtcgatg aaattgatcc atcaagcaga gcgttacttg
     9661 gcggaaaaag cctatcggac acagaaaaaa gaatttcttc ctaagacagc agttacaaat
     9721 aggaaggaga ataaaaaaga acgccagctc tttgcaaaag gtgatcggat atttgtcaat
     9781 gaataccaaa aagaagcgtt agtttatgaa gatataggtg aagatacgat tgatgtatat
     9841 ttagacaaaa aaataatcca cgtgcctcgt cagcgtgttc gtctggtgcg ttcagcagaa
     9901 gatctgtacc cgacaggtta cgatttagac agtctgttta tcgattataa aacacgtaaa
     9961 cggcagcgtg acctagagcg aggatcaaaa aaagctcata aagtactagt aaaagaaatg
    10021 cgcaaacgac aggaagaaag aagagtaaac gatgaaaaca gcaagtgatt atctcaaatt
    10081 agctcataat atcagtggct gtaaagaaaa ataaaaacag tgaaatcatt ggttttattc
    10141 cccgtgtaag ctaaaataaa aagaaaagag tgctgaggag gtagggcgat aaaatgaaat
    10201 taagcacaga ctggcgtaaa gaaatacaaa cgattccgaa tcttttatcg atattccgta
    10261 tttttttgct gccgatttat ctttattttg tgctgagaca gtcattttat atagcaggag
    10321 cggtcattgt tgtctcaggt ttgtctgatt atctagacgg ggtcattgct cggagatata
    10381 atcaggtaac agacttaggg aaggtgcttg atccgtttgc agataaattg acacaacttt
    10441 ttctgatttt gtctatggca tggtatcgac cgtggctttg gctgttgttt ggtctattct
    10501 tgataaaaga aggctttatg tttgtagctg gattaatcgg gctatctaaa aatatcaaat
    10561 taagtggagc gaagtggtat ggcaaagtag cgactgctgt catctatgtt ggcatgatcc
    10621 ttttgctgct tttcccagaa ttaccaacgc tgtgggtgag agtcattttt gccgtcatca
    10681 cttacggtct tttacaatct tttgtcttgt atgctgtcga gtaccgaaag atgtttcaaa
    10741 gaaaataaaa aaggggtgtg acctatgtcg cattcctttt tttgataaat agactcccag
    10801 tagctacttc tcgataataa gccaaccaaa ccaaaaacag gagaagttgt ttttgaaatt
    10861 atttcttgtg attcggagtt aaatggtagg ctcacaacct cttggtaaac ggtgttcaat
    10921 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat tttcccgttt
    10981 ttttctctaa tacttaggtc aaaccgctga tttcacaacc tcttccttaa acggtgttca
    11041 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    11101 ttggctgttc ttattacttg atgcaggaca gcgttttcac aacctttgat tcacggtttt
    11161 ccagaagtag cttcttcgaa aaataagccg aaatttcttg aagctgatca cttctgtcgc
    11221 aacctctact gactaaggct tgatataagc ataaccttgg gattgcagtt caactaaatc
    11281 aaggacacct gctggtacaa cgattacttg ttcaggtaaa gaactttctg aaatatgatt
    11341 agcacgcata gcattggcgc aagcgtgaaa aacgactcct tgcaaatcta agaattctgc
    11401 attggcagag tctagataac cggtaatcgc gatgccattt acagatacga caatcgtcgt
    11461 ttctggagaa gcttttatca ggttttttac gtttttccct gtttctgacc atttttctaa
    11521 ttcatcaata tggaaaacga ctttcattga aatcattcct ttacttttta ataatcatct
    11581 tgatcgcttg ttctagttta gccgcttgtt cttctggatt actatcaggt ttctcaaagc
    11641 aatttttcag attatctgca acgatcatgc ccattacgcg atcaatacta gaacgaacag
    11701 cgcttaattg agtgatgaca tccatacatt ctttttcgtc ttcgatcatt ttttggatac
    11761 cacgaatttg tccctctgta cgtttcaaac ggttcaggat atttttatta tcaatctcca
    11821 taacacattc tcctctattt ttataattag ataatacccc ttatcggtat tttatgcaaa
    11881 tataaaaagt agtgaaaaaa atgaagagtc attctggttg acatattaat aaagctcctg
    11941 tttaatatac cccatgaggt atttataaaa atgtaaatga ggtgcagaaa atgaatttgc
    12001 tatttcaatc aataaaatcg ataacagtcc cagaattaaa ggaaaagctt ttggaaaatc
    12061 cagcgttact tgatgttaga acacctgcag aataccgggg cgggcatata aaaggagcta
    12121 aaaatgttcc attacaaagt ataaacagat atgatggaga caaagaaaag accgtctatg
    12181 tgatttgtca atcagggatg aggagtaaac aagcagctaa agaattaaag aaatctggtt
    12241 atgatgtagt gaatgtacgt ggtggcatga atcaatggtt tgatcgaaca gtaggaggga
    12301 aataagatga aaatcgttat tgtcggaggt gtagcaggtg gaatgtctgc tgctacacgg
    12361 cttcgccgat taatggaaga tgcagaaatc gttgtttttg aaaaaggacc gtatgtttct
    12421 tttgcaaatt gcggcttgcc ttattatctt tcgggagaaa tcagtgaacg ggaaaatctt
    12481 cttgtccaaa cgccagaatc attatccgct cgtttttgtt tagatgtgcg tccaaatcat
    12541 gaagtgacag ccatctttcc cgaaaacaaa acggtagaag tcgtacatga gggtcaaaaa
    12601 cacattgaac agtacgatgc attggtttta tctcctggtg caaaaccagt tgttccatcg
    12661 attccaggga taacagaagc cgacaatgtt ttttctatta gaaatgtacc agatatcgat
    12721 aaagtgatgc atgcattaga aaaacagcca aagcgtgccg tgatcgttgg tgcaggattc
    12781 atcggattgg aaatggcaga aaacctaaaa agaagaggtt tagaagtcat ggtgatcgaa
    12841 caagcaccac atattcttcc aacactggat gaagaaatgg cagcttttat agaaaaagaa
    12901 ttgtctcatc aaggagtaga agtgattact tctcatgctg tcgctggatt tgaagaccac
    12961 gggaaacgat tgcgactgga tgatgggcgt accatccctg ctgatttagt tattttatcc
    13021 attggtgttc gtcctgataa ccagctagca gtgaccgctg gaatcgaatt aggcatacgc
    13081 gggggtatcc tagtagacga acgttatcaa acgaatattc ctgatattta tgcggtgggg
    13141 gatgctatcg ttgtaaaaca gcaaatcact ggaaaagatg cacttatttc tcttgcttca
    13201 ccagccaatc gtcaaggtag acaagttgcg gacacgattt ccggaatttc tcgaagaaat
    13261 caaggcggta ttggaacagc aattatacga acgtttggaa tgactgccgc atccaccggt
    13321 ttaagtgaaa gaacagccaa agaaaacgaa ctgtcttttg aagtcattca tgtatcagga
    13381 aaagatcatg caagctatta tccagaagca acagatattt tactgaagtt gatcttccat
    13441 ccagagactg gcgagattta tggtgcacaa ggtgttgggg caaaaggtgt ggataaacgg
    13501 atcgatattt tagcaacagc aatcaaaggg catttgacga tcttcgattt accggaatta
    13561 gaattgacgt atgcaccgcc atttggctca gccaaagatc cagtaaacat gctaggatat
    13621 gcagcaatga acattgcaga agggcttagt gaaaccgtac aatggcatga attgccgaca
    13681 gaactagcaa aaggaaaaat tttattagat gtgcgaacag cagaagaatt ggaaaagggc
    13741 aaattcaagg aagccaaaca tatccctttg aatgaacttc gggaccgatt aaatgaatta
    13801 gacagccaac aagaatatat cgtcagctgt catagcggat tacgtagcta tctagcggaa
    13861 agaatcttga agcagtctgg ctaccacgta aaaaaccttg atggtgcatt ttctttatat
    13921 caaactgtcc gacctgaaga actgatatat cctaacaaat gatactcgtt aaaaagcttg
    13981 cattcgactc ttaaaagagg taaactaatc ggttccacca ctttaaaaaa agaggagaat
    14041 gtgtatggtc aacacacaaa aacagttaga agaagcatat ctgaagaacg tgtatcaaaa
    14101 gctaattgat aaaaaaaacg aactgcggga atcgatggat acggcaaaaa tagaaggaaa
    14161 ccagtcgttg aaagaaatgg ctggcgagct tcgattgaat tttgacagtt acttagataa
    14221 cctggatact ttttcgatga ttgaaatgaa aaatagagaa atcgatcaga tgaatatcaa
    14281 acagaaaaac gcagaaaatc agctagcaaa gatcgaacgt ttattgaaaa gtccctactt
    14341 tggaaaagtc gttgtagact ttctggataa tgagccaaaa gaatcctttt atatcggtac
    14401 agcaaatttc acagatgatg cagatgaaaa tcttgtttat gattggcgtt caccaattgc
    14461 cgaacttttt tacaataaca tgattggtcc atcttcttat acggttcgtc aaaataaaat
    14521 cgatacggtt attaaagaaa gacgacaatt tcttttggaa agagatcgtt tggtcaagtt
    14581 ttttgatacg tctgtagcta ttcaagatga cgtattatta gaagtcttag gccaagatga
    14641 gaccaatgaa atgaaggcta tcactgctac gatccaatcg gaacaaaaca caatcattcg
    14701 ggatgtgaaa agtcaaaata tcttggtaaa tggcgtagca ggaagtggta aaacctctgc
    14761 tatcatgcag cgtattgcgt atctgctcta tttatatcga gatacgatga catccgatga
    14821 cttgctgatt ctatcaccta atcatcggtt tatcgactat atttcgaatg tcctaccatc
    14881 attaggtgaa agaaatccac tgaatctaac catcatacag ctggtttcac agctttcagc
    14941 agaggaaata gaaggtgaag aggcttattt caaacgtatc acgggagaaa atgtatcgga
    15001 gcagacagaa cgtttgcgta gcaaaaagtt tatcgataat ctaaaacaat cagatccttt
    15061 gtttttagat catccgaatt ttatccgtgg acttactaaa aatggaaaaa cagtcctttc
    15121 taaaaaaacg atcgaaaaaa tatatgagaa agttcctgct catcctaaat tgatcgatag
    15181 gcttcaagca acgaaaaagg cactgatgag cgaatggaag aatcatcttt tgaaacaagc
    15241 aaaaagtcct gctgtgcaga atcaggtact ctcgttgaca gaagataggc agttggaatt
    15301 attcggtaaa ttgatttctg atgattcgga acaaagtatt gtagcttatg caaggaaact
    15361 tttacaaaaa aaatatcgaa aaataaccag acaaatcgaa gaaatggcat gggtggatga
    15421 gcatcagttg tttgaaagaa tttatgaaaa acgatatggc tcagcttatg cttggcaacc
    15481 cacacgtact gttgatgaag cagtcattat attagctatt cgtcatttac ttgttgaaaa
    15541 ggtaaatgta ccagcatttc gctatctatt gattgatgaa gtacaagatt ataccctggc
    15601 acaattaggt ctattgatcg aattatttcc aaaaactcac tttactttag taggtgacga
    15661 gaatcaggca atctttaatt cttccactac ttttgctgat attatgcgtt gtttcgatga
    15721 ctaccatctg ccgatccatc ggtatgatct tagaaatagt taccgttcta gcggagcaat
    15781 cacagagctt ttcaaaacct atgctgtgga tcaagagaag atcgacatcg tatcgatccg
    15841 accgcaagga aaagaacccg aataccgctc ttttcgatcc tctgaagaat tgttgaaaat
    15901 actagctgag attttggttt ctctaaaagg agaaaaagcg gcagttatca cccaaacaga
    15961 agaggaaacc caaacgctac aagaatcgat taaaaaaaat tcatatggat tagaaaactg
    16021 ccaaatcatt cctttgagtc tagcaaaagg actggaattt gatcatgtaa tcttatatcc
    16081 atttgaaaac gatggagatg agcaaagaag acgcagacaa atgtacacgg cgatatctag
    16141 aggaatgaaa agcattgtcg tattggaaag agcaacataa ttaagagata aaaaataaaa
    16201 agaacaactg ataaaaagaa caaaatattt ttcatttgct gatgagaaat gttttgttct
    16261 ttttcttagg aaaaatttag gaacacttta tgtgaaaaga aatgaaatgt atggtaaact
    16321 cctacgtcgt aacaaaaaaa cgtgaggtga ggatatgaaa aaagtaacaa gagaagaagt
    16381 tgcatcaatt ttggtgaaag ataagtattt ttcaaaaggt aattactggt taaagatttg
    16441 gcaaacgtta gtggctcttt taggatggat gatcgttgtt cttcctttta tatgggtctt
    16501 tttcccatta acgagaccag aaagagccaa ggatttttcc ttgtacgctt ttttatcaga
    16561 aaagaaaatg ctgtatttct taatcggctt ttttgtattg atttttttca gtttcctgat
    16621 tacctcattt gtattgacta ggtggaacaa tcgaaatcta tacaaaaaag taaataaatc
    16681 ttttgaa
//
LOCUS       DACEDD010000064        16625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEDD010000064 DACEDD010000000
VERSION     DACEDD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.064"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(207..821)
                     /locus_tag="IWO45_002352"
     CDS             complement(207..821)
                     /locus_tag="IWO45_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333809.1"
                     /translation="MNMADRIQYLRKSKGISQEELADKVGVSRQAVSKWESEQSTPDL
                     EKIIIMSDFFGVTTDYILKGIEPVADKEQKNKELTSKVLYISSTAFVAIGLFCAFGNW
                     YAEQTMEAVWGSMIIQAIGIVGYFIARILSEEKSPFYVNWLNIIGVAFMPISMITGYI
                     SGLVFKLEGWIAPYPIGIFHTLVFVLVFFVVVIASYIILKKQTK"
     gene            complement(902..1090)
                     /locus_tag="IWO45_002353"
     CDS             complement(902..1090)
                     /locus_tag="IWO45_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333810.1"
                     /translation="MVGNQEEERKSVYFSRQKNVKGGVLIFVVADIWNCYNMDMFKLR
                     YKQEYSADLSKNIIATAS"
     gene            complement(1191..1862)
                     /locus_tag="IWO45_002354"
     CDS             complement(1191..1862)
                     /locus_tag="IWO45_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333811.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNYPTFDTLEKIMDVLGVTPNELLSGEWKYIDHTEPYIMDIIKREQDFNVSLDYLS
                     ENEFFNDEKECRFYMETKLIQYIHNYITNEVTELEELMEIKQLIQRQKIERMMKVHKE
                     MRGLDRYREQPKEYKYHDPYDDWIFRQLADIDSRNNIPDTSPQLDFDETDYEDYLKEK
                     WNRGL"
     gene            2258..3418
                     /locus_tag="IWO45_002355"
     CDS             2258..3418
                     /locus_tag="IWO45_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0333812.1"
                     /translation="MINKGKNTIFTRKKRGIRKEQCTITTHKNLSVKIDYISIVFETA
                     TAEDIIMHILDLPTDIFNVYPAMIKFKTYQARWQIGDIYVSGDARKTEDNPQGLGCYL
                     VMTGRGCDDIFRILDSRNYTFGDMFRRCERRYGLDNFHFTRLDIAIDDKNEKPFFTIE
                     QIKKKCEKEEFISNSEGYHFDESKFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSKH
                     NKTLDEVGSWKRTEMQLRDDKAHVFAMTFKDRPLELGELAFGLLANNLRFVVQNRNES
                     NKSRWKTCRFWERFLGAVEVLKLQVPKLHNSLEETQQWLTEGGVISAVKSFYFLEEHD
                     ALGGLEKVGTMLDRARYSSSLSSKLTAHLQRIDRTDLIPYIQYDTKHGKGGI"
     gene            3418..3621
                     /locus_tag="IWO45_002356"
     CDS             3418..3621
                     /locus_tag="IWO45_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0333813.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEKVIDKLDAI"
     gene            3700..4893
                     /locus_tag="IWO45_002357"
     CDS             3700..4893
                     /locus_tag="IWO45_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0333814.1"
                     /translation="MKEKRRDSKERILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQRKQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFNFKLSEIIENDTKEKVVLTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVVIDHQLLKSKEQGYYIETPKTKSGTRQVPLSR
                     ETIQAFQRVIRKRTKGKLIEIDGYKDFLFVNPKGNPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(4901..5200)
                     /locus_tag="IWO45_002358"
                     /pseudo
     CDS             complement(4901..5200)
                     /locus_tag="IWO45_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5358..6254)
                     /locus_tag="IWO45_002359"
     CDS             complement(5358..6254)
                     /locus_tag="IWO45_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0333815.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(6247..6927)
                     /locus_tag="IWO45_002360"
     CDS             complement(6247..6927)
                     /locus_tag="IWO45_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0333816.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(6928..7710)
                     /locus_tag="IWO45_002361"
     CDS             complement(6928..7710)
                     /locus_tag="IWO45_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0333817.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(7703..8620)
                     /locus_tag="IWO45_002362"
     CDS             complement(7703..8620)
                     /locus_tag="IWO45_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0333818.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(8784..9107)
                     /locus_tag="IWO45_002363"
     CDS             complement(8784..9107)
                     /locus_tag="IWO45_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0333819.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            9895..13122
                     /gene="fss3"
                     /locus_tag="IWO45_002364"
     CDS             9895..13122
                     /gene="fss3"
                     /locus_tag="IWO45_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0333820.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            13235..13549
                     /locus_tag="IWO45_002365"
     CDS             13235..13549
                     /locus_tag="IWO45_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0333821.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            13562..13936
                     /locus_tag="IWO45_002366"
     CDS             13562..13936
                     /locus_tag="IWO45_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0333822.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            13946..14281
                     /locus_tag="IWO45_002367"
     CDS             13946..14281
                     /locus_tag="IWO45_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0333823.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            14364..15704
                     /locus_tag="IWO45_002368"
     CDS             14364..15704
                     /locus_tag="IWO45_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0333824.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            15782..16456
                     /locus_tag="IWO45_002369"
     CDS             15782..16456
                     /locus_tag="IWO45_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0333825.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
BASE COUNT         5397 a         3110 c         3280 g         4838 t
ORIGIN      
        1 ttgattatcc ctataaatat tacactttca agcatttcga agtatagaaa aacactcatt
       61 ttaccacgaa tataccacga ttgaacgagc cttgatatga cgataaaata gcactaaaaa
      121 gacaccattg acacattgaa gtggtgtctt ttattaataa gcagtcaatc ctaagactaa
      181 ctgcttttgc aaaataaagg taatcactat tttgtttgtt tctttagaat aatatagctt
      241 gcaatcacaa ctacaaagaa tactaacacg aaaactaaag tatgaaatat tcctatcgga
      301 taaggtgcaa tccacccttc tagtttgaaa accaatcctg aaatatatcc tgtaatcata
      361 gaaataggca taaaagcaac tccgattata ttaagccaat ttacataaaa gggggatttt
      421 tcttccgata gtattctcgc tataaaatat ccaacaatcc ctatagcttg aataatcatt
      481 gaaccccaaa ctgcttccat tgtttgttct gcataccaat taccaaatgc acaaaacagc
      541 ccaatagcaa caaaagctgt tgatgatata taaagtacct tactcgtaag ctctttattt
      601 ttttgttctt tgtctgctac tggttcaatt ccctttagga tatagtcagt tgttactcca
      661 aaaaaatcac tcattataat tattttttct aagtcaggcg tactttgttc actttcccat
      721 ttggaaacag cctgccttga aactccaact ttatccgcaa gttcctcttg tgatattcct
      781 tttgatttcc ttaaatattg tattctgtct gccatattca ttagctaaca ctcctttctt
      841 tcatggtgta attttagcaa tattatcagt tgcatagcca ccatttagat atggaaatat
      901 gtcaactggc agttgcaatg atgttttttg acaaatcagc agaatattct tgcttataac
      961 gtaatttaaa catatccata ttataacagt tccatatatc cgccacaaca aaaatcagca
     1021 cacccccctt gacatttttc tgtcttgaaa aatacacact ttttctttct tcctcttgat
     1081 taccaaccac agaaaagaaa aagccctgtc aagagccttg ccaccattgg tggtgctttg
     1141 cactctttac tgggcttttt gtttgctgta tctttcattc aagaggaaaa ttaaagacct
     1201 ctgttccatt tctctttcag ataatcttca tagtccgttt catcaaaatc taactgtggc
     1261 gaagtgtcgg gaatgttatt tctgctgtct atgtccgcaa gttgacggaa tatccagtca
     1321 tcataagggt catggtattt atactctttg ggctgttctc tgtatcggtc taaccctcgc
     1381 atttccttat gtactttcat catgcgttct attttttgac gttgtatcaa ctgcttgatt
     1441 tccataagtt cttctaactc cgtaacctca ttggtaatgt agttatggat atactgtatg
     1501 agtttggttt ccatgtaaaa tctgcattct ttttcatcat tgaaaaattc attttcagac
     1561 agataatcta aggaaacatt gaagtcctgt tcccgtttga taatatccat gatatatggt
     1621 tcggtgtggt caatatactt ccattctcct gataataatt cattgggcgt tactcccagc
     1681 acgtccatta tcttttctaa tgtgtcaaag gtaggataat tcacaccccg ttcaatcttg
     1741 gaaaggctct gcatattgat accgatttta tccgcaagtt cctgttgttt cattcctctg
     1801 tgttttctta tggtctgtat attctctcct aagaaactga ttttcttttc gtaatgttcc
     1861 ataacttagt ataaccgctc ctttcgttgc ttttctcggt gtttataagc atatcatact
     1921 taatatttat taaaaagtca atcaaaactt cttgacaagt aattctaatg gcgttattat
     1981 cgtattagat agggtgatta agcacgctag ggtcaatcac acgcttgagt aagcatagaa
     2041 aagatgtatt ttcattttga tagcatgaat aagcatggac tatcagaccg aaaataaggt
     2101 ttctgactgg ggctgttccg tttagccacg agtcttacaa ggctcgtgag cgttaagaac
     2161 ggatcagaca caggaagaaa gggaacgcct ttaggcgttc ataaagggcg tatatgtaac
     2221 accgccctgc gtatacatgt catagtacct agaaactgtg ataaataaag gaaaaaacac
     2281 gatttttacc cgcaaaaaac ggggcatacg aaaggagcaa tgcactataa ctacacacaa
     2341 aaatttatcc gttaagattg attacatcag cattgtattt gaaacggcaa ccgctgaaga
     2401 tataatcatg cacattttag atttaccgac tgacattttc aatgtttatc cagcaatgat
     2461 aaaattcaag acttatcaag cacgctggca gattggagat atttatgtat cgggggacgc
     2521 aagaaagaca gaggataacc cacaggggct aggctgttat cttgttatga ctggcagagg
     2581 ttgtgatgat attttccgta ttctcgacag taggaattat acctttgggg atatgtttcg
     2641 acgctgtgag cgaagatacg gactggataa ctttcatttc acaagacttg atattgccat
     2701 tgatgataag aacgaaaagc cattctttac catagagcag ataaagaaga aatgcgaaaa
     2761 agaggaattt atttctaata gtgagggcta ccactttgac gaaagcaagt ttgatgattt
     2821 cgacaccgca aagactgttt atatcggtgc tggtaaatcg ggattatcct accgctttta
     2881 tgataaggat aaggaagtct gttcaaaaca taataagaca cttgatgaag tcggcagttg
     2941 gaaacggaca gaaatgcaac tgcgtgatga taaggctcat gtttttgcca tgacattcaa
     3001 agacagaccg ctggaacttg gggaactggc tttcgggcta ttagcaaaca acctacgctt
     3061 tgtcgtgcaa aacagaaatg aaagtaataa gagcagatgg aaaacgtgtc ggttttggga
     3121 acgattttta ggggctgtgg aagtcttgaa actgcaagta ccgaaactac ataattccct
     3181 tgaggaaaca cagcaatggc tcacagaggg tggcgtgatt tctgctgtca aaagttttta
     3241 tttcttagaa gaacatgatg cattaggtgg actggaaaaa gtgggaacta tgcttgacag
     3301 ggcaagatac agcagttccc tttccagtaa attaaccgcc cacttacaga ggatagaccg
     3361 caccgacctt atcccctata tccagtatga cacgaaacat gggaaagggg gtatctgatg
     3421 aataacaacg atattcccgt atgggaaaaa tacaccctta ccattgaaga agcgtcaaag
     3481 tatttccgta tcggagaaaa caagctaaga cgattggcag aggaaaacaa ggacgctggc
     3541 tggctcatta tgaatggcaa ccgcatacag attaaacgcc gacagtttga aaaggtcatt
     3601 gataaattgg acgcaatcta atgcaaatga gccttgtatg tgttatgatg aatacaagtc
     3661 atatcaaggc tctttccaac aaggaaagga gcagacacca tgaaagaaaa aagacgtgat
     3721 agtaaagaac gtatcctgca tactggagag agccaacgaa cagacggaaa atacttatat
     3781 aaatatgtag acgcatttgg aaacacaaaa tatgtgtatg cttggagatt gacacccaca
     3841 gacccgacac caaagggaaa acgggaaaaa ccctcacttc gtgaactgga acagcagata
     3901 agacgggata ttgaggacgg tatcgacagc acaggcaaga aaatgacgct ttgccaactc
     3961 tacgccaaac agaacgcaca gagggcaaat gtgaagaaaa gcacacagaa acaacggaaa
     4021 caactcatgc ggttattgaa agaggacaag ttaggtgcta ggagcattga tacgataaaa
     4081 ccctctgacg ctaaggaatg ggcgttacgc atgaaagaca aaggcttttc ctataacact
     4141 attaacaacc ataaacgctc gttaaaagcg tcattctata tcgccataca agacgattgt
     4201 gtaaggaaaa acccttttaa tttcaagtta agtgaaatca tagaaaatga taccaaagag
     4261 aaagtagtat tgacagagga acaggaacaa gccttactgt cattcatcaa gacggataac
     4321 gtgtatcaca agtattatga tgatgtgctg atactgttaa agacaggact tcgtatctcg
     4381 gaactgtgcg gactgacagt agccgatatt gatttcaaga atgaggttgt ggttatcgac
     4441 caccagttat taaagagcaa ggaacagggc tattatattg aaacgcctaa gacaaagagt
     4501 ggaacaaggc aagtgccatt aagcagagaa acgatacagg catttcaacg ggtcataagg
     4561 aaacgcacaa aagggaaact gatagagata gacggataca aagattttct gttcgtcaat
     4621 ccgaaaggca atcctaaagt tgcgattgat tacaacgcct tatttgtccg tatggtaaag
     4681 aaatataaca aacaccacaa ggataacccc ttgccacata tcacaccaca tacgctacgc
     4741 catacgttct gcacaagact ggcaagcaag aacatgaacc cgaaagattt acagtatatc
     4801 atgggacatt caaacatcag tatcacaatg aactggtatg ctcatgcgtc catagatacc
     4861 gcaaaatcag aggttcagcg tctaatcgca taagaagtat ttaccacgat tttaaccacg
     4921 cttgatagcg aaaatataag aagatagacc tagatatgtg aggtttacca caaaagcaaa
     4981 atgcccgtag agcctataaa ataaggattt gcggacattt aagaagatat aaaaagatag
     5041 tcaaaaagac atatatgatt tctgataatt ctttgtaagt tgtgtgctca ttttcaatca
     5101 aaaagtatag tattttcatt tgccgttcgc taaattcgat gcccattatc ttcacctcct
     5161 gaatacagct taagtgtaca attattatct ctattttcat acagaagaaa gttcttgatt
     5221 aatgtagaag tttctaatat tcttgattat ttcaaatcaa taaaaaagcc tgcaatattt
     5281 atatcacagg cttttttctc tattaaatat atataagata atacattttt ctactagttc
     5341 tcactaatct tgtataatta aaccctccgc aacttgacgg agatggagaa gatgcccccg
     5401 tctaatctag cggtcatctc tcctcccatt ccttccacca atttttttga caggtaaagc
     5461 ccgattccgc tagattccgt atttgtgcgg ctggtatttt cggtataaaa acgctggaag
     5521 actttttcaa cagaggtttg cgttcctggt ttgataggat tggccactgt taatcgaatg
     5581 aatggatcct cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg caataaattc
     5641 tgtatgatat tttgcagcac tcgcagcaac atgagcggat ctgctaatac agttgtattt
     5701 atcgagtgtt cagcgaacgc tacctcgata tttttctcag aaaattgttc ataaaaagag
     5761 aaaaatactt cttccacctc tttagaaaaa gaaagcgtat ggagatccat ttctttttgc
     5821 ttcgtctcta ttcgagcaag atcataaaac tgatccgtca tactgagtaa tcttgcaaca
     5881 gattgtttga tgatcgttaa atacttctcc cgtttctccg tatcctctac ctgttccagt
     5941 aattgtacat aaccgttaat agcagtcaaa ggagttctga gatcatgaga aaggccgacg
     6001 atcgatagtc tgatgttttc ttccatttct tgaaatgctt gtcctttttg ttcattttca
     6061 tccaaccatg tattcagtgc atctactagg tccattagat tcttttctcg aaattcgact
     6121 gaaagacgac caccatattc ttgtcgttca gaaagagtgg cgattttctt agttaatctc
     6181 ttgatttccc tttgcaggaa gaaaataaaa cttcccataa agataacaac gagaagcaac
     6241 agccatttaa tcaaatctaa accccactcc ccacaccgta tgaatataca cagcatgtgc
     6301 ctttgctagc ttccctcgaa gattggaaat atgcatattc actgttttct cactatcaaa
     6361 atatggttct tgccaaactg tttcataaag atttgctcgc gaaaatactt ttttcggatg
     6421 ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc aaatggacag ccacaccatt
     6481 cacatttaca tccctcgact ctttgtccag taagatttcc ttgtaataga tgcgttgcga
     6541 cagatctttt acttggacag aagtctgatg acgaagttgg atgccgattc gtaacaaaag
     6601 ttcttcgata tcaaatggtt tcgtcacata gtcatccgct cctgctgcca acaaggaaac
     6661 tttatctctt tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg
     6721 gatttctgct aacaatgctt cacctgaaag ccctggaagc atgagatcta gaaggatcaa
     6781 atcgacagga tatgtgctta acaataattt tccttccgta cctgaataag catcaagaag
     6841 tttgtatctc tgttcaagaa cctcctccag taaggaatga acagcttcat cgtcttcgat
     6901 aatcaaaata gtttccagca ggaccaccta cttaatgtca gatttttgga aaataaacat
     6961 tcctagagct gatgatacca cgattgttac tagactgacc agaatcattt tcggtgcttg
     7021 cgaataagtt gcttgttgcg caaagtccgt atacatgagc cacgtttccg taaaccactc
     7081 cgttagaaat tccaagcctt tgatttttgc gaaaacaccg ggaatgaagg ccgttcccat
     7141 gatataaaga aaagaaagaa gcaaactgac actggtactt ctagtgatga atgtcaaagc
     7201 cactggaaca gataatgtcc ctaatgtcag cagaagcaat acaggaaata cttttagaac
     7261 ataatttcca tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc
     7321 aagcatggct agtcccgtga tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag
     7381 aagtttcgac acataataag tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt
     7441 accgttttga aattcttccc caatataaaa gcacgcaaat gctgcgaaag ccagaagaat
     7501 ccatgcagcc attttttcta ccattaaaat ccctgttgca ccgttgatga ctgtatccgt
     7561 tgcttggttt aattcattgc cgtattttgt atgcatatac cattcgttcc aagaataaaa
     7621 aagcggaagt acgaccatca gtcctagtga gacccacatt ttacggctct tccatacttt
     7681 tattttttca gcagttaata gattaaacat gtggattgcc tccagttaat tgtaagaaat
     7741 aatcttccag tttttgccca actaggtgaa taccatccac aggaactttt gcttctacca
     7801 atgcttgatt gatggctgct acttggtcaa ttccttcaaa aagctggata acacggctag
     7861 attgtacaaa ataatcccta taaccaagag agtcgagaac cgttactgct tgttccgtcg
     7921 ccgaagtgtc aatttttata aattgacgag attcttgtgc caattcttct ttcgataatt
     7981 ctttgatcaa gtgtccttca tgcaaaatac catagtgagt ggcgatctgt gacaattcat
     8041 ctagcaagtg gctagaaagc aagacggtga tgccccgttc ttttaccaaa cgttggatga
     8101 tttctcgcat ttctgccatc cctgctggat ccatcccatt cgtcggttca tccaaaatca
     8161 aaaattctgg attcgtgatc agtgcatttg caatagctaa acgctggcgc atacctaaag
     8221 agaaattctt tgctttcttt ttccctgtat tttccaatcg catcattttc agcaggtctt
     8281 cgatttctct atccgacaca ccgccgttcg ctgcttggat acgtaggtta tctcttgcag
     8341 ttaattctgg ataaagagca ggggtttcaa tcgattgacc catacgtcgt cttgctgcct
     8401 gcaaatccgt cgttccaaat aattcgatcg aaccttcatc aatcgagatc aaacccataa
     8461 tagttctcat aaaggtagac ttacccgctc cattttcccc aatcaatcca tagatcattc
     8521 cacgtttgat ttcgattgat actttatcta atgctttggc agcaccgtat tttttggtta
     8581 taccagttgc tttcaagacc gtttcatttc caaaatccat acttatcctt ctttcttatt
     8641 gatgagtgaa gtatagttga cgggtataaa gaaagcagcc agaaatgtat aaagaaagtg
     8701 taaattttcc aattgaatgt caaaaaaaac aatcggacaa tacgtgtctt tcctattatc
     8761 aggcagtttg ttatacgctt gtatcagttt cctgattctt tcttttcgac caaagtcgtt
     8821 tctctgtatt cctcttcact gaaaattttt gcttctttca gatcaagctt gagtaatttt
     8881 accagaccgg ttataaggat aactatagca agactccctc ccagaaaaat tagactgtct
     8941 cgcagacgtg gttgcgttgt cttccaatca aagagcgcac caaacagtgc taatccaagt
     9001 ggactggcca ccatggtaat cgttgttatc gttgacataa gtcgtccttg tatatgcgtt
     9061 ggaacttctc gctgaagcca gaccatataa aaagcaaaag aaaacattaa tccttaaaaa
     9121 cggacttatg aaaaataata catgccgttt gggatttaca attagctacc aaataagagg
     9181 ttatgacaaa agacttttca caatcccttc ttccgaataa acggtgttct cctgccaact
     9241 cctcggtcac aagtcacaat atttatcaaa catgagaagc aatttttgta aattccttct
     9301 tatttcttga agctgaccac ttctgtcaca acctcagaag cggataaata atattgtttg
     9361 atgatacttt atctttttcc cgtttgttta tagatcgaat gatattatag accaagaatc
     9421 ctacctatta acatagctaa aaacacaaaa aaggcacttt ccaaattgtg gaaagtgcca
     9481 gaaaagttag gattaaggta actgcgctca gcattgcttc gcccagtacg attctcgtaa
     9541 ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga actgtgaagc tttacgggct
     9601 ttcttaagac ctggtttttt cttcgtatca ttgaataagt ttgaaaaacc ttgatttata
     9661 aaggttttcc cgtaaatata ttttattata tttgattaaa tttgaaatgt ttgtgttcta
     9721 aaatgtgttc taatttaaag gcagtatatt tcgtatttta gattaaattt ttaggctcat
     9781 tcattctcaa atccattcta ttcagtcaat aaaaatttta aaaagacttt ataaacaaag
     9841 atgttcaagc gagcatctta tttttttgtg cttaatttga aaggagaaca tttcatgtat
     9901 aaaaaacaaa aaaagaaatt atgtgtatta actattttga gtttggctgt tcaagcaatt
     9961 gttggagcaa ttgctcctac gattactttt gctgatgaaa ttacccatcc acaaacggta
    10021 accgtagaac tagatttagc acatcaatat gctgttgaag gaacttttag tgatggtcgt
    10081 ccaatgtcag aagtcactgt tccgcattat gcagtgtaca acggctttaa acaagatatt
    10141 ttctgtattg aaccaggagt cccgatttac aatgagttca ctcctggcta tgagaaaaac
    10201 ccgttaccag atatgtccga taaagccaaa ttagtttccg ttttatggaa aaaagtaggt
    10261 acagatgcag atactcatat tgtggcacag aagatgatct ggcaagaagt taatgggtat
    10321 acccttcact caataaaacg atcagatggt agtgccgtaa atatcgcagc cattgaggca
    10381 aaaataaatc aagccattga cgattatcag aagaaaccaa ccttccataa tagtacggct
    10441 aaaacagtat tagggcaatc gactactgtg acggatacga ataatctgaa tttgtcagag
    10501 ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc gtgtgaatgg taatcagttg
    10561 ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa gaaatctgct
    10621 ggtaccggaa cacctgtcgc ctataaaaag gctggccaac agactttaat ggccggggcg
    10681 attgataaac ctaataccta cacaatcaaa attgatgtgg aaacggaggg ttctttaaag
    10741 atcaaaaaag tcgataaaga atcgggtgat gttgtaccag gaacggtttt ccatttagac
    10801 tttggaaaaa ctttacctgc aaaagacgtg actactgaca aagaaggcat tgctacattg
    10861 gatgagattc ctcatggtac aaaagtaacc attaccgaaa aatcagtacc ggcgccttat
    10921 acgattgata ccacccccat gactaccacc attaaagcag gcgaaaccat ttacgtgact
    10981 tcaaaaaacg cacgggaaaa agggcaaatc attctggata aaagcggagt tgaaacaggg
    11041 agtgatcttt ggaatgacaa ttattcctta gctggtaata cgtttgccat tcgcaaagac
    11101 agtccaaccg gtgaaatcgt tcaagaaatg accacggatg aaaacggtca tgcggaaaca
    11161 ccaaaagaga ttgctaatgc gttggaattg ggaacctatt atgtgaccga aactaaagct
    11221 agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg aattgaagta tgccaatcaa
    11281 accgtagccc ctgttaccag taacgtaaaa gggcaaaatc aagaagtcac tggcgaaacc
    11341 actttgacga aagaagacaa agatactggg gataaagcac aaggtaaggc agtctttgag
    11401 ggtgctgaat acaccttgtt tactgctaag gatggaaaag ctgttaaatg gagtgaggct
    11461 tttaaaccag agttagcgaa gggaacgaaa ggttctgatg aaacggtcac tttgacttta
    11521 gacgaaaaga accaagttgc cgttaaacat ttagctatca acgagtatta ttgccaagaa
    11581 acaaaagcgc ccgaaggata tagccttgat gaaacaaagt atcctgtatc catcaaaaag
    11641 gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg ttacggcaaa agaacaagtg
    11701 attcgctttg gctttgattt ctttaaattt gctggatcag ctgcgggcac taccgaaact
    11761 ggcttcaatg atctgacctt taaagtgtcg ccattggaag ggaccaatga aatcacaggt
    11821 gccgaagatg aagcgaccac agcttataac gagcaattag ggtttgatgg ttatggcaag
    11881 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaagcccc agaaggtttt
    11941 caaaagatta cgccattaga aatccgttct acttttaagg aaaacaaaga agactttgtg
    12001 aagagtgagt atgtctttac cattaccgaa caagaccaaa aacaaccaat taaaacggtg
    12061 accgttcctt acgagaagct gacgaacaaa gcattttctg ttagtttgaa ccgtttaatg
    12121 ctctatgatt tgcctgagga agaagatagt ttaacttctc ttgcgacttg gaaagacgga
    12181 aacaaagaat taacttctct ggattctacc gagctagttg ataaattgag ttacaatcta
    12241 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga tgcgacaaaa
    12301 gctgcccaag aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac aagcgcaacg
    12361 ttggcgaaca aagagaaaac aggaacttgg aaaattcagc ataaattaac cgccgaacaa
    12421 gttttaaata aaactattgt cttattcaac tatgtgtatg aaaataagga agcttttgaa
    12481 gcaggcgata agccagtagc gaaggatgtt agcttgaata accaagccca aactgtcagt
    12541 tgtacagtgg aacaccatgt ttctatccaa acaaaggccc acctagaaaa cggttctcag
    12601 acctttactc atggtgatgt ggtggatatg tttgatgatg tgtcgattac tcatgatgta
    12661 ctggatggct caaaagaagc tttcgaaaca attctgtatg ctttattacc ggatggtaca
    12721 aacaaagaaa tttggaaatc tggcaagatt gattatgaag taaatgataa agaattcacc
    12781 aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac caagtttact
    12841 tttgcggaaa tcaattacga taaagatggg aatatcaatg gaaaacacaa tgaagatttg
    12901 aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa ccatactaag tacgccgaaa
    12961 caaccggaaa caccaactgt tccaagtggc tctcaagaat ctagtcccac agtgaagacc
    13021 ttccctcaaa ccggggagaa aaattccaat gttttactgt tcattggttt tactttgatt
    13081 tttgcgacgg cgggctatta tttctggaat cgccggaact aaggtgatga gatgatacaa
    13141 gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc gctatttttg
    13201 tatccaaaac taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg tcgtgccaga
    13261 tatggctgaa acctttggaa agttaagtta tgccggtgaa ggagaaatta taacagaagg
    13321 atatggacgg aatactacgg tgattggtcg cagttatcat ttgtattcca gtaagcaacg
    13381 ggccgatgat attgaggtgg tagtagctgc ggaagccggt gaaaaggatt ttgatcaaga
    13441 ccaaccgcta aaagccgtga atccccattt ggttgccaaa ggctatgaga ttgagaatcg
    13501 tgggtttact gattacgtgt tgtatgttga tgatttagtg aaagcatagg aggaaaagaa
    13561 gatgagatta acagaaggta ttgtcgtaga ttcaggttta acatttggga agttgcgttt
    13621 ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacaattg gtaatgaggt
    13681 aaaagaacga acctataatt taaaatcctc tgcacaaggg cgaatgattc aagttagtat
    13741 cccagctaat gtacccttac gcgagtttgc ttatgatgca gaggtagagt tggtcaatcc
    13801 aatcgtggat accgttgcca attatgtttt tcgagaagga actaccgtta attggttcat
    13861 taaggcggat gatttagtct tgaagcggca gccgaatcaa ggaaatccta cgaaccaaaa
    13921 tgaaagaaag aaataggtga agattatgga aattaatgtt gaacatattt tagattgtct
    13981 cgatcaatat ggaaaaggcg agctgactga ggaacaactc gttaaagcat taacctctga
    14041 tgaaaaaatg tttctgatta tacatcaagg tttacttgga gaaaatagcg acagtgaaga
    14101 agactctgat gtactagact ggttaggaga agaagagtct ttctttatag ttgtcgaagt
    14161 aaatgaaaac ttatgtcggc aggcagaatc cattttggaa gaaattggtg tggagatgcc
    14221 ggatgctatt gaaggctttt taaatcaatt ggttgaaacg aaacaactcc cctcctcgta
    14281 gcagtaaatg actagcataa ctttttgaaa ttgcctaatg aaatcctatt gggctttttc
    14341 gattggaggt ggaaaccatc aaaatgtaca aaggacatcg catacgcgca ggagatcagc
    14401 acttggttta tcactttgtt cttggctggc tactcgctct gttcatcggt tggatgagcg
    14461 tcttttattt tcaagagttt agacaatttg atatttctaa gctatcactt tctactattg
    14521 aaatcgtttg gtctataaaa gatttagtat gtctgcttgg aagtctcgct ttttcaggag
    14581 ctatgatatt gctttatatt cacttttttc ttgatcattg gcgaagcttg tggcatcgac
    14641 aaaagctagc tcggatgatt ctagagaacc attggtatga agtgaaacaa actcaaagtg
    14701 aaggcttttt caaagatttg aatagtagcc gaaccagaga gactatcagt tacttcccca
    14761 aaatctgtta tcgaatgaaa gatggattac tgtctattcg cgttcaaatt tcacttggga
    14821 aataccaaga tcagctttta aaattggaaa agaagttaga aagcggactg tattgtgagt
    14881 tagtagaaaa ggaactcaaa gactcctatg tggagtatac tttactgtat gatatgatcg
    14941 ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg gactctgcga ttgatgaaaa
    15001 atcaagtatg ggcttatgat tctttacccc atatgttaat cgctggtggc acgggtggag
    15061 ggaagaccta tttcctcctc accattatcg aagcactatt gaagtcagat gcagaattgt
    15121 ttatcctcga tcccaaaaat gcagatttag ctgatttagg tacggtgatg cctcatgttt
    15181 attcacaaaa ggaagaaatt tctgcttgtg tggaagattt ctatgaacgt atgatggctc
    15241 gtagcaaggc aatgaaagaa atgtctaact acaaaacagg agagaattat gcctatcttg
    15301 gacttccacc caacttttta atctttgatg aatacgtggc ttatatggag atgttaacga
    15361 cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat cgtgatgttg ggtcgtcaat
    15421 ctggtttctt tctgattttg gcttgccaaa ggccggatgc gaaatattta ggagacggaa
    15481 tccgcgatca attcaacttt cgcgtggctt tgggtcgtat gagtgaactc ggatattcca
    15541 tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca catcaaaggt cgaggttatg
    15601 tggatacagg tggcagtgtg attagtgagt tttatacgcc acttgtacca aaagggtatg
    15661 atttcttaga ttcaattagt aaattagaaa agatgaatga ttgagtgatc ttcaatactg
    15721 actttatact tcaagtaacg tataatactt ttatatagta ggaagaaagg ggataattaa
    15781 tatggaaagt aatgattctt atttagagta tgcaaaagca aataaggata agtttatttc
    15841 ttatattatc aaagggaaat cccccgaagt tggacgagac gcagttttta tggcagggag
    15901 tcctggggct ggaaaatcag aggttgccat tggattagct gagaattatg ataaccatgt
    15961 gattattgat gcggattact ttcgcaccca gtttcctgat tataatggaa aaaactctag
    16021 tctctttcaa aaagcatctt cttggttggt agagcaggca tttagatatg tcttagatca
    16081 tggctattct tttattctgg atgcaacttt tgcaatatta agtgctgaaa aaaatatctc
    16141 gcgggcgttg aaaaaacatt ttcgagtgac gattttctat gtttatcaag accctaaaat
    16201 cgcttggaaa tttacacaag aacgtgaaat tgcagaggga cgtcatgtgc cgaaaaaggc
    16261 atttattaac gcttttttca aatcaagaga gaacattgca aaagtaaaga gtaaacatcc
    16321 agatgtattg cttcatatca taataaaaga ttatacgaat aatatttccg aagttcatta
    16381 tgctacggat aatctagaat tatttttacc actagggtat agtaggaaag atttggaggt
    16441 tcaattacat gactgagaaa gaacaagtaa cgaaaattgt aaagaaatac aataagagca
    16501 ttgcggattt atcggaaaat gctactgcaa aagagttcaa aacggtgatt aaatatgtag
    16561 ccgatcaggc caatgaaaaa caacggaaat tagttggatt aaataaaaaa tagtaggaca
    16621 aatta
//
LOCUS       DACEDD010000065        16614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEDD010000065 DACEDD010000000
VERSION     DACEDD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.065"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWO45_002370"
     CDS             414..947
                     /locus_tag="IWO45_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0333826.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="IWO45_002371"
     CDS             complement(1437..1961)
                     /locus_tag="IWO45_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0333827.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="IWO45_002372"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="IWO45_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0333828.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="IWO45_002373"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="IWO45_002373"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0333829.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="IWO45_002374"
     CDS             complement(3683..4363)
                     /locus_tag="IWO45_002374"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0333830.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="IWO45_002375"
     CDS             4489..5358
                     /locus_tag="IWO45_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0333831.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="IWO45_002376"
     CDS             5384..5899
                     /locus_tag="IWO45_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333832.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="IWO45_002377"
     CDS             complement(5946..6509)
                     /locus_tag="IWO45_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0333833.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="IWO45_002378"
     CDS             complement(6564..6743)
                     /locus_tag="IWO45_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333834.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="IWO45_002379"
     CDS             complement(6820..7329)
                     /locus_tag="IWO45_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333835.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="IWO45_002380"
     CDS             complement(7425..7805)
                     /locus_tag="IWO45_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0333836.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /locus_tag="IWO45_002381"
     CDS             8135..8884
                     /locus_tag="IWO45_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0333837.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..9235)
                     /locus_tag="IWO45_002382"
     CDS             complement(9068..9235)
                     /locus_tag="IWO45_002382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333838.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(9251..10345)
                     /locus_tag="IWO45_002383"
     CDS             complement(9251..10345)
                     /locus_tag="IWO45_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333839.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="IWO45_002384"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="IWO45_002384"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0333840.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="IWO45_002385"
     CDS             complement(11267..12211)
                     /locus_tag="IWO45_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0333841.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="IWO45_002386"
     CDS             complement(12213..12899)
                     /locus_tag="IWO45_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0333842.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13372..13692
                     /locus_tag="IWO45_002387"
     CDS             13372..13692
                     /locus_tag="IWO45_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0333843.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            13853..14758
                     /locus_tag="IWO45_002388"
     CDS             13853..14758
                     /locus_tag="IWO45_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0333844.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14755..15528
                     /locus_tag="IWO45_002389"
     CDS             14755..15528
                     /locus_tag="IWO45_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0333845.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(15705..>16614)
                     /locus_tag="IWO45_002390"
     CDS             complement(15705..>16614)
                     /locus_tag="IWO45_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0333846.1"
                     /translation="AISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVF
                     VGIEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPA
                     MGYVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNK
                     NGAPTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQR
                     KEWVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHL
                     DKILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         4895 a         3402 c         2968 g         5349 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcgtggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaatta actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttattttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata
    12961 tagttgtttt ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttattat
    13021 ataattcacc ttacttattg taagataaag ctttttttaa tttattgcct gctaatatat
    13081 aaaaataagg tctagttact gtttttttaa atgattaata aataacaact cctttctatg
    13141 aaattgcaaa gcattcatct tcttttctgc taacgatata tccgtcaatg cttgttacac
    13201 tttactaaaa agtatgagat tgccgatatc tggataggtg tgctatacta cagaaaaaga
    13261 tatacgatct gccaccgggt agtaaaaaag aggatgatca ttccattagg tctttgaagg
    13321 gatgattctc ctctttttgt gtatttttta tattgaagga ggaacgtatg tatggcatgg
    13381 atggaattaa tcatagcagg aattttggaa gtattctggt caacgatgat gaaatggagt
    13441 gatggttttt ctaaaatcaa ttattctttt tacacagtga ttggaatgat tgctagtttt
    13501 tactttttgt caaaggcaat caaaagtttg cctatgagtc tctcttatcc tatttggaca
    13561 gggattggcg cagttggttc ggtcattatt ggtgtggtct tgtttcatga taagctgaat
    13621 ctttccactt ggttctttgt cgggttgctg ctcatcagca tcattggcat caaaatcact
    13681 agcggtcact agatacttag acaagcagac aaggactgat cagaaaaagc ggggcttttt
    13741 atttgatatt cctctttcta tgaaaaaaca agaaaataag actaatgacc tagcgacttc
    13801 tctactcatc aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag
    13861 tctttcggtc caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag
    13921 ttttagctta tctcctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac
    13981 aacgatcatg aaagctattt taggactgat ccattactca gaaggaacga tccgtattgg
    14041 tgaaaatgag atttctccct ctagtcataa aggattagaa caagtaggag cattgatcga
    14101 atatccgggt atctatccat tcttaacagg ttatgatcat ctgaagttat tctctgaaac
    14161 aaacgatgta tctgctatcg acactatcgt gaaacagctc aagatggaaa aatacattca
    14221 caaaaaagcg aaatcctact cgctgggaat gaaacaaaaa ttgggaattg ctcttgcctt
    14281 actgaaccat ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc
    14341 aacaagagat gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat
    14401 ctccagtcac ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca
    14461 aggaaaaatc atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat
    14521 cgttatctct acgacaaatg atcaagctgc tcgaacgatc ttgacagacg ctggatatcc
    14581 cttggaagat cctgataaaa tcaagcttct aaaaaaaaac gaagagttat taggaactgt
    14641 tatccagtta ttgtctgcag aaaatgtcca tgtgacagat gtccagcatc tgacgaatga
    14701 tttggaatct tccctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgacg
    14761 ttgcttataa aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct
    14821 gttctattag ttgctttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc
    14881 attgatccag ttgaaatgac tgctcagctt ttcagtgcca catcttggat tgtctttatc
    14941 atgattgctg ctgcaagtac gatcatttca atggaatcac aatatggtac attgaaaaat
    15001 ctcctttatc gaaagtattc tcgaggagaa atattagtaa gtaaatggat cacattagtt
    15061 atttattcta tttatttata ccttttagct attattgtca ccgtgctgat gaaactgatc
    15121 cttttccctt cgatttcctt tacggagaag atttctactg gtcagacact gatccaaagc
    15181 ttattcactt atactttggg tagttatatc ggtttatggc taattttaag tttagtgttg
    15241 atgattgctt gttttatcaa tagttccggt gcctccatct cagcagggat cgtcttttat
    15301 tttgcttcat caatcatatc tggtatcctc atcgcactca ttcaaaaatg ggagtggatc
    15361 aaatggaatc caatcagtat gctgaatctg caaaaccaaa ttggcaacga agagattatg
    15421 aagcaattga ctcatctttc tactaatcaa atgctgtttg gcaatttagc ttacattgct
    15481 ttgttcttag cattgggata cctcgtattc aagcgaaaaa atgtatgatg gttcattaac
    15541 aaagaagttg tgacagaagt ggccagtttc aagaaatttc ggcttatttt tagaagaagc
    15601 tacttcttga acaccattta ttcaaaaaaa gaagcgatga cacgaatgtt gtcaccgctt
    15661 cttttttgaa taaaccacag gcttcacagc ctctctttat acttctatac gtgaagcatc
    15721 ccagtggcaa ataaaaccat tgcgattact ccgaccatcg tcacaccgat aagtaaaatc
    15781 ttatctaagt ggatatgttg tacaggttcg ttttgttctt tacgtgcttt gaggtaaaaa
    15841 taaatacctg gtaagaaaag aacaagaaca agcatcactt cattaaaggc tacaactagc
    15901 atggcccata cttgaaaagc acttgctaaa aaccctacta aaaattggac ccactctttc
    15961 cgctgataag aaagcttcat ttggtacagc cctaagaaaa gccacgtgaa gaaaatcgct
    16021 gctgtaccaa gtgtataagc aaagttatat gcactgtcag tgaataataa tgaaagtaaa
    16081 aagacattcg tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg
    16141 agttttcccc atgctttagg caaaagtccg tcctcagcca tattccccat tgtttcaatc
    16201 ggcaagattg tccatgagag ccaaacccca actaaagaaa tgatcaaacc gccgttgatc
    16261 acgaccgccc cccatttacc aaccatcatt tcaaatacat atcccatagc aggttgcgct
    16321 ttgatcgttt gcagctgatt catcgtcatt gtgccatatg gaagcatgga aaccaagacg
    16381 taaataatga tcaaaccgaa tattcctaga atcgttgctt tcccaacatc cgattgtttt
    16441 tttgctcgtg aacctacgac agaagctcct tcaataccaa cgaataccca gagcatcacc
    16501 atgatgcaac tcaatacttg actagaaaac gggatcggct gcccattcgc attcaatatg
    16561 atatcgctag aaaagttatt aatgaattgt tggatcgaaa aggtgctgat agct
//
LOCUS       DACEDD010000066        16587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEDD010000066 DACEDD010000000
VERSION     DACEDD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.066"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..786)
                     /locus_tag="IWO45_002391"
     CDS             complement(79..786)
                     /locus_tag="IWO45_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0333847.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(865..1203)
                     /locus_tag="IWO45_002392"
     CDS             complement(865..1203)
                     /locus_tag="IWO45_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0333848.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1314..2276)
                     /locus_tag="IWO45_002393"
     CDS             complement(1314..2276)
                     /locus_tag="IWO45_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0333849.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2291..3622)
                     /locus_tag="IWO45_002394"
     CDS             complement(2291..3622)
                     /locus_tag="IWO45_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0333850.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3630..4310)
                     /locus_tag="IWO45_002395"
     CDS             complement(3630..4310)
                     /locus_tag="IWO45_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0333851.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4529..5008)
                     /locus_tag="IWO45_002396"
     CDS             complement(4529..5008)
                     /locus_tag="IWO45_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0333852.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5135..6814)
                     /locus_tag="IWO45_002397"
     CDS             complement(5135..6814)
                     /locus_tag="IWO45_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0333853.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7362..8864
                     /locus_tag="IWO45_002398"
     CDS             7362..8864
                     /locus_tag="IWO45_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0333854.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8877..9539
                     /locus_tag="IWO45_002399"
     CDS             8877..9539
                     /locus_tag="IWO45_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333855.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9640..9843)
                     /locus_tag="IWO45_002400"
     CDS             complement(9640..9843)
                     /locus_tag="IWO45_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0333856.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWO45_002401"
     CDS             complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWO45_002401"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0333857.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWO45_002402"
     CDS             complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWO45_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0333858.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13807..14421)
                     /locus_tag="IWO45_002403"
     CDS             complement(13807..14421)
                     /locus_tag="IWO45_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0333859.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15027..15404)
                     /locus_tag="IWO45_002404"
     CDS             complement(15027..15404)
                     /locus_tag="IWO45_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333860.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWO45_002405"
     CDS             complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWO45_002405"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0333861.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4808 a         3573 c         2831 g         5375 t
ORIGIN      
        1 ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc aaagcgctga
       61 tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga tcaagcgcaa
      121 cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg caaataatac
      181 gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca aaatttggat
      241 tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa gtaatgctaa
      301 agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca atacacctac
      361 cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa gtgctgcacg
      421 aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc
      481 tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac tcaacatctt
      541 attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac cgacattatt
      601 tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat tgtttttgct
      661 tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa ttccggaaat
      721 aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca ttccataacc
      781 atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag taggaaaaga
      841 atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt tcagtttcat
      901 cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta aacggatgtt
      961 cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca agtctttaat
     1021 cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata ccactaatcc
     1081 gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt ctgggcaaat
     1141 caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat ttggtaatgt
     1201 catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa agaagatctt
     1261 gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa gtatcattct
     1321 gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat ttcctctact
     1381 tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc atgatggcgt
     1441 ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta ataccctgaa
     1501 ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg aagtgaaacg
     1561 atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg cggaaggatt
     1621 attcttcctg caccattttc atccacctgg atattttgat aaagataacc ggaaagttta
     1681 gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc aaatggataa
     1741 tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata caaaaagcgc
     1801 cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt catctttaaa
     1861 gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact tgcttttgta
     1921 tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat tttgatcagt
     1981 ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt atcagtcacg
     2041 atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc taaaaagcga
     2101 agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact ttcccgtaat
     2161 agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg
     2221 ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac tgtcatttca
     2281 ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg gaaacgatgg
     2341 tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt tccaatacac
     2401 gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt aggttcactt
     2461 gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg acatcagaaa
     2521 tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta
     2581 ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta tcaatcgtat
     2641 ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa
     2701 gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg
     2761 aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca gaggcaatac
     2821 tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg atctggtttg
     2881 gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga tggcggttga
     2941 cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat
     3001 ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg acaagcatga
     3061 tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg actgtaaatg
     3121 tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt gcaatctcag
     3181 cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat gctaattttt
     3241 ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag cgttccatgg
     3301 catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat cctgccgccc
     3361 cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat
     3421 gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt gaaactagtc
     3481 cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca ttcaattctt
     3541 ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata tattctaaaa
     3601 tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga gacgctcatt
     3661 atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg ttcaatgaca
     3721 gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg cgcagccttt
     3781 gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc gacatcaata
     3841 tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc tgcatgataa
     3901 atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata ctgaacgata
     3961 ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac gccgaatgga
     4021 aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct gttcgcataa
     4081 tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat aggaatcata
     4141 tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt tacttcttct
     4201 gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac gcgttggcca
     4261 ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat attttacacc
     4321 tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac atcaatgaaa
     4381 aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac aaagcaattg
     4441 taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt ttgaggtctc
     4501 tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa ataggatgga
     4561 cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca ccgcttgaca
     4621 caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg
     4681 cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc attaatacaa
     4741 aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt tttttgtaat
     4801 ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt cctttcacta
     4861 cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct tctgtgaaag
     4921 attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt tcttctaaat
     4981 aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat cgtttattat
     5041 aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga gcaagacgag
     5101 cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga cgttgagtag
     5161 cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat aagtaacggc
     5221 ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg ctttctaaat
     5281 gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca cggctagctt
     5341 ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc ttttctcgcc
     5401 gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt cgatcacgta
     5461 aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt tctgcacttg
     5521 tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg cgtccagtaa
     5581 tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga gcagctaatt
     5641 cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg atcattaatt
     5701 gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc aacttcactg
     5761 ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc atcgcgatgc
     5821 ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta cggtgtgtac
     5881 cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt tctaaaatga
     5941 tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga tcttcacttg
     6001 gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag
     6061 tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc actcgttgca
     6121 aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc gtatcaaata
     6181 cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca tttgccgatt
     6241 cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca taatctgttt
     6301 gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg atactacctt
     6361 cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta cggaaaaagc
     6421 tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg aaatccttaa
     6481 atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc gtcaactctg
     6541 tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg atcgcatctg
     6601 catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct aaatacgtaa
     6661 aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt agcccacaat
     6721 ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca ttttcacgaa
     6781 cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct tatgacatcc
     6841 aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc aatgaagcat
     6901 atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc agcacatccg
     6961 cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa aatggatctt
     7021 ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc attattatac
     7081 aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca cgtaaaaatc
     7141 cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt cgtcggtttg
     7201 aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact ttttatatat
     7261 acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct ttaagatagg
     7321 ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg
     7381 catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc gtgttcacag
     7441 ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa gaagcagcga
     7501 atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga gaagcttcat
     7561 ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt ccagatccag
     7621 gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct gaattagata
     7681 atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat ggaataactg
     7741 ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca ggcggtgaac
     7801 ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa atcagtggtg
     7861 tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc acggatggtg
     7921 aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct actctagcag
     7981 aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa gatgaagata
     8041 ccacttatga aatgacgatc aaaggaattt atgaatccag cgaaacaagc ggcagtatgg
     8101 gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac acttttgcaa
     8161 atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac actctatctg
     8221 atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac acagatacat
     8281 ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg
     8341 caagtttttc taaaaatgtt gttttactag tgactgtagc cggtattatc atacttactc
     8401 tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag
     8461 gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt tgtatgattc
     8521 ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc
     8581 tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc ggtggacaaa
     8641 tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga accacgaata
     8701 atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa cctgcacaat
     8761 tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta
     8821 tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt
     8881 tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt tataaagatg
     8941 tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt ggttctggaa
     9001 aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga aaggtatact
     9061 atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga
     9121 ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac gtcatagcag
     9181 caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta gatcgactgg
     9241 ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt tcgggaggtc
     9301 agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta atcgtagcgg
     9361 atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag
     9421 aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa
     9481 aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa
     9541 aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga tatggttcgg
     9601 attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt cgtcttttcg
     9661 agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga tgtcagcacc
     9721 tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc
     9781 ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt ttaaatctgc
     9841 cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac atcaacaaca
     9901 gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt tgattaaaaa
     9961 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
    10021 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
    10081 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
    10141 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
    10201 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
    10261 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
    10321 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
    10381 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
    10441 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
    10501 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
    10561 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
    10621 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
    10681 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
    10741 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
    10801 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
    10861 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
    10921 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
    10981 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
    11041 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
    11101 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
    11161 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
    11221 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
    11281 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
    11341 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
    11401 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
    11461 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
    11521 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
    11581 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
    11641 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
    11701 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
    11761 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
    11821 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
    11881 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
    11941 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
    12001 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
    12061 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
    12121 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
    12181 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
    12241 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
    12301 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
    12361 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
    12421 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
    12481 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
    12541 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
    12601 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
    12661 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
    12721 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
    12781 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
    12841 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
    12901 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
    12961 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
    13021 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
    13081 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
    13141 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
    13201 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
    13261 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
    13321 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
    13381 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
    13441 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
    13501 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
    13561 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
    13621 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
    13681 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
    13741 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
    13801 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
    13861 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
    13921 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
    13981 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
    14041 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
    14101 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
    14161 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
    14221 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
    14281 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
    14341 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
    14401 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
    14461 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
    14521 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
    14581 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
    14641 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
    14701 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
    14761 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
    14821 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
    14881 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
    14941 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
    15001 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
    15061 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
    15121 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
    15181 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
    15241 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
    15301 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
    15361 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
    15421 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
    15481 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
    15541 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
    15601 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
    15661 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
    15721 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
    15781 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
    15841 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
    15901 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
    15961 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
    16021 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
    16081 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
    16141 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
    16201 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
    16261 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
    16321 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
    16381 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
    16441 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
    16501 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
    16561 gaatgactgc ttttttgacc ttcactt
//
LOCUS       DACEDD010000067        16365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEDD010000067 DACEDD010000000
VERSION     DACEDD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.067"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..723)
                     /locus_tag="IWO45_002406"
     CDS             complement(85..723)
                     /locus_tag="IWO45_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0333862.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(846..1478)
                     /locus_tag="IWO45_002407"
     CDS             complement(846..1478)
                     /locus_tag="IWO45_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0333863.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1829..2128
                     /locus_tag="IWO45_002408"
     CDS             1829..2128
                     /locus_tag="IWO45_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0333864.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            2623..4788
                     /gene="acm"
                     /locus_tag="IWO45_002409"
     CDS             2623..4788
                     /gene="acm"
                     /locus_tag="IWO45_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0333865.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(5060..5545)
                     /locus_tag="IWO45_002410"
     CDS             complement(5060..5545)
                     /locus_tag="IWO45_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0333866.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            6025..6666
                     /locus_tag="IWO45_002411"
     CDS             6025..6666
                     /locus_tag="IWO45_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0333867.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            6685..7320
                     /locus_tag="IWO45_002412"
     CDS             6685..7320
                     /locus_tag="IWO45_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0333868.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            7338..8153
                     /locus_tag="IWO45_002413"
     CDS             7338..8153
                     /locus_tag="IWO45_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0333869.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            8472..9908
                     /locus_tag="IWO45_002414"
     CDS             8472..9908
                     /locus_tag="IWO45_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0333870.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            10097..12544
                     /locus_tag="IWO45_002415"
     CDS             10097..12544
                     /locus_tag="IWO45_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0333871.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            12774..13841
                     /gene="rlmN"
                     /locus_tag="IWO45_002416"
     CDS             12774..13841
                     /gene="rlmN"
                     /locus_tag="IWO45_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0333872.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(14034..15137)
                     /locus_tag="IWO45_002417"
     CDS             complement(14034..15137)
                     /locus_tag="IWO45_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0333873.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            15690..16238
                     /gene="aac(6')"
                     /locus_tag="IWO45_002418"
     CDS             15690..16238
                     /gene="aac(6')"
                     /locus_tag="IWO45_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0333874.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         5673 a         2647 c         3328 g         4717 t
ORIGIN      
        1 atccattgat acatctgtct ttttgtttaa aggaaataaa gtatgctttc ttagcaatag
       61 agcatgtatt atttctataa aaatttagga gaataaaacg actagcaata tagctgtgaa
      121 atcaaatatc acatgaactg cccaacttgt aatgatcgag ttgcttttaa tggcagcttg
      181 gttgaataac aatcttgcca ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat
      241 atttccatta tcataagtac tgtaatggat aaatccaaag atgattgctg acaagaaact
      301 agcgacagga atgctgaact tactagaaaa aatagcgaga aaatacactg agaacaattc
      361 ttctccaagt atcatgatag gcaaaataat aaataataac gggctgaaag cctcatttat
      421 tggattgcct gttaaacccc atttcaaaat ttgttgcaaa aataccgaga ctgcggcact
      481 gattactaca ttgataaaaa aatatttggc tatatttttg acaggagcga caggtttcga
      541 gaacattttt ttcgtatctt ttttaccaaa aacaagtgta aatgctataa atccagtgat
      601 agttaaagaa aaaatgctaa taatggatgg cgcaaatagc aaccctaaca aaccgagtac
      661 aacgacaata gtgcccaata tccctttttt aagttctatt ttttttccta aaatgttatt
      721 catgataata actcctacta ttcagttttt gaagatagag tcagtataca tgaagagtta
      781 aaataggcaa tgttttttta tggatatttg tattgctaaa aatttatctt atatattcat
      841 tttgattata ctacttttat ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga
      901 ttaagcgtac aattttttag atgttccgag cgtacttcaa tcatttcaaa ggagttgcta
      961 cttaaatcga tattttttaa atcagtatca aaaaagctag cattttctaa atcattagta
     1021 gaaaacatta ttttagtgag ggaacagtct acaaaatctg cgcttgataa gacagagtgc
     1081 tcaaaggtac tttcttttat gtttgaacca gataaattta catagtccat tttacaattc
     1141 tcgaataacg tgttcccaat ataactttcc atcaatttag tacctgttaa tttacaattg
     1201 acaaatttca cgcgatgcaa catgcttttt gtcaatatac agcctgaaaa atcacagttt
     1261 tcgaatatac agtcggtaat ctccattctt tgaaaatcat tggaagcaaa aaatgcatac
     1321 ttgaaaatag atttgtaaat aattggatgg cagattattt ggttttcgat gacctctttt
     1381 tgtgaaagga tattatacag ttcaaaatct tcgttttcat agatatgaaa aaaggatcct
     1441 ttattcaagc ttgcttcatt aactttagga aaaatcatat ttaattacct ccattttcat
     1501 aaagtcatat taacaaaagc taaaccaact tacattagca gaaaatgaga taggttttcg
     1561 cctttttcga taacgatagt gtcgctatct cttgaaacgt tcttagaatt gccctgagag
     1621 acacaaatta ggcgtaattc taataaaaat cttagaataa aaatttttac tgatttaatt
     1681 tagaatgttt ttaaaataaa aatcgttgcc ataacagtgt attgaaaggt tttaacaatc
     1741 cagtaatgat ttaaaaaaat ctagatgtag cgtatacttt gtttatagaa tgtagtggga
     1801 agaaatatat ttttgggagg aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt
     1861 actaaaatta tatgatctcg ttttaaatcc agagatcaaa gaaaaggaac gtatactttt
     1921 gatagacgca aagacaggct tagaaaaagg tcagtattat cctaaagtca taaataattt
     1981 ggaacgtagt ttacgtccgt tagcaatcag gggagaattg tctaaaccag tatcaccgtt
     2041 ttatatggag atatcaacaa taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc
     2101 ggcaccgatt acttttgggc atttataaaa acgagttatt catctcaatg aattaaaaag
     2161 tatagtcaat ctcataatgc aggctaggtt catttatttg aacagagtct gtattttttt
     2221 attctaaata aagaaacaga tgtttcttta tattgtattt tatcctttaa ttaacacaaa
     2281 aacagctagt ataaactaat aatgttaatt aaaagtgtat tttatgaaac tatcgttatt
     2341 tattaatgtt gcatttttgt aagttaaaaa ttggaaatat agattgaaaa aatgaaagca
     2401 ataagtaaaa taaaatgaat atatgcgaaa aaataattta cattaggaaa aaccttgtaa
     2461 aaacagtttt ttggcatatt tattttttat aaatatttgt aaaataacta tcaatttttt
     2521 agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg agagatgata
     2581 tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat
     2641 attattttat caatgttatt tattattact aatataacat cactgattcc tgttcatgtc
     2701 tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg
     2761 aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat
     2821 atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa
     2881 ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc
     2941 agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa
     3001 ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg
     3061 ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct
     3121 tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact
     3181 aagcacattc gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt
     3241 aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat
     3301 caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta
     3361 caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat tgaaataact
     3421 ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa
     3481 aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa
     3541 cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga
     3601 gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt
     3661 ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag
     3721 tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta
     3781 ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt
     3841 tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt
     3901 tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact
     3961 ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt
     4021 cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc
     4081 caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac
     4141 ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat
     4201 gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa
     4261 gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa
     4321 cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac
     4381 agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg
     4441 acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata
     4501 ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt
     4561 agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa
     4621 agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa atcaaaaagc
     4681 attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg tttattgata
     4741 gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt agaatataat
     4801 attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa
     4861 gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat ttggctaact
     4921 atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt gtttgattgg
     4981 ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg aattgggaaa
     5041 taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct aagtaaacaa
     5101 gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata tttggatgaa
     5161 agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt tttttctctt
     5221 tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg aggtcaggaa
     5281 gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct agttgtttta
     5341 aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct tttcttgatt
     5401 tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt ttgactgacg
     5461 taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt agttcataag
     5521 cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt agtgtatcat
     5581 aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt aaaacaaatg
     5641 acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg gtggaaagag
     5701 gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat agtaggaaaa
     5761 tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc tggcttttcc
     5821 gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat cttcggatca
     5881 aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa
     5941 aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca agaggatttt
     6001 tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc gcaattatta
     6061 gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc attaccatta
     6121 gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta cttgcttcaa
     6181 acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt tattttttat
     6241 gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat tgcctttatc
     6301 ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc gattcctcaa
     6361 ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc
     6421 gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt tatcaacttg
     6481 gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa
     6541 atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat catctatctt
     6601 acattgactg gtattctaac aatcataatg aaacaagttg aaggaaaatt aaattattac
     6661 cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa agcatttggt
     6721 gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac attagctatc
     6781 gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg cttagaaagt
     6841 tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga gcttaaaatg
     6901 aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca tctgaatatt
     6961 tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga agaatacacc
     7021 aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa tatctatcct
     7081 tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt ggctatgaat
     7141 ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt acgccagcaa
     7201 gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat tgtcacccat
     7261 gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc aattcattaa
     7321 gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc gcaggtcttc
     7381 ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg actatcaacg
     7441 aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga tttagagata
     7501 aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt gaacaatatg
     7561 ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg
     7621 gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc
     7681 aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa
     7741 tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg tcatctggtt
     7801 atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac caagatgctg
     7861 ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg gtcgatggtc
     7921 tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa ctagaagact
     7981 tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca cgcaagtcag
     8041 atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa acaggtgaat
     8101 tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat taacaaatag
     8161 gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg ttctcctgcc
     8221 aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa
     8281 aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc attttcacaa
     8341 aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac ataattttat
     8401 tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg ttataggaaa
     8461 ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt ctgtggggct
     8521 ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa ggagaatcag
     8581 tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac ggtgatttgg
     8641 cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa caaggtctga
     8701 aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga ggagaagtca
     8761 atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa aacgatattg
     8821 aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat gaatataatg
     8881 gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca ttatttgagg
     8941 cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc ttcacttcat
     9001 taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga atgtatgaag
     9061 ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa
     9121 tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt gacagtaatc
     9181 ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat tggatggatg
     9241 tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa aaaggatggg
     9301 caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca gattttcttg
     9361 ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga gttggtgctg
     9421 gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt cctggaatgt
     9481 ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag attgacccag
     9541 aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg gtactgatta
     9601 cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc catgacgact
     9661 atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt tcggatggag
     9721 cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg ctgaacggtt
     9781 atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa ggatctttgg
     9841 aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa aatggagaga
     9901 acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt gccttttctt
     9961 gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg
    10021 tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg aaacttgatg
    10081 aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga aaaagacgaa
    10141 aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg ttgggtttgc
    10201 cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa tattgattgc
    10261 aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt tattagccgt
    10321 attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa accgacgctg
    10381 gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc atttatttgg
    10441 caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt tagccagtga
    10501 tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag caatccttta
    10561 tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag tgctattact
    10621 ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa atggtatcca
    10681 atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc aagctcgaaa
    10741 gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt
    10801 agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa aagcggtgag
    10861 ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag agccggagca
    10921 gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga atctagagaa
    10981 accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag atgagttgcc
    11041 agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa atcaagacta
    11101 tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa gtgatgagta
    11161 taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga
    11221 tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag tccagccggc
    11281 tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc ttgctttagc
    11341 ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg gtattgaagt
    11401 accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac aaccaaacca
    11461 cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg tacaaacggc
    11521 tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg ggaagtcagt
    11581 agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg aagtaaaact
    11641 gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac ctcatctgtt
    11701 gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga
    11761 aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt ctggttacaa
    11821 tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct taccattcat
    11881 cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg aagttgaaga
    11941 tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga tcctagctac
    12001 tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac cttccagaat
    12061 ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta atggggcaga
    12121 aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata aaccgatccg
    12181 tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat ttgttacaga
    12241 tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg aaacgacagg
    12301 tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg tcgttgaaat
    12361 gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca accgagcagc
    12421 acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag gaagcaagcc
    12481 tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg ataatgacat
    12541 gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac aaggcatgat
    12601 gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga tgtcttgtct
    12661 ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt gcattaagga
    12721 aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat actttggaaa
    12781 aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata gaaaacggcg
    12841 agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt gtaagtaatt
    12901 tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag cattttatca
    12961 tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta aaatatttgt
    13021 ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa tatggcttgt
    13081 cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt gcaagcgggc
    13141 tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc atgatggtcc
    13201 aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga
    13261 tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc atcaatgatg
    13321 ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga ttagcacaca
    13381 aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc
    13441 cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca attgagaaat
    13501 tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg tttgaatata
    13561 tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca gatctattaa
    13621 aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc agtgaacatg
    13681 atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta ttgaagaaaa
    13741 acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca gcttgcggac
    13801 aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct actcgttttt
    13861 tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt aaataagaga
    13921 aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct catcaagcaa
    13981 agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat tatttaactg
    14041 attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac gtcaagaatg
    14101 gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc atttgttgcc
    14161 atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat gtcggttaac
    14221 gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa atcgaactgc
    14281 tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg aaggtatccc
    14341 tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc agaattatag
    14401 ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga aacgacttgt
    14461 tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt accataagtc
    14521 aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt gtagcctaga
    14581 tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc gccaatacct
    14641 gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca
    14701 ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata gatgacacat
    14761 accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac tggtttagct
    14821 agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac tgcaaacagg
    14881 acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc ataagttgaa
    14941 ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc tacttttatt
    15001 ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt tgtcccatat
    15061 tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata atttggtaag
    15121 aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt aaaattttaa
    15181 agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag acaggataag
    15241 tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa aaacgaacaa
    15301 gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat ttctaccgat
    15361 tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt atatgaaaac
    15421 aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg
    15481 atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat
    15541 aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga
    15601 tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt
    15661 agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc
    15721 cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag
    15781 acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg
    15841 tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt
    15901 gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat
    15961 tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta
    16021 cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg
    16081 ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag
    16141 gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga
    16201 tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag
    16261 taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa
    16321 ttccaactta tttccgaaga agctactttt ggaacaccgt gtacc
//
LOCUS       DACEDD010000068        16100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEDD010000068 DACEDD010000000
VERSION     DACEDD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.068"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..357
                     /locus_tag="IWO45_002419"
     CDS             <1..357
                     /locus_tag="IWO45_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0333875.1"
                     /translation="DSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTT
                     SDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILL
                     VIGLRITFMSYKKSKR"
     gene            complement(512..1498)
                     /locus_tag="IWO45_002420"
     CDS             complement(512..1498)
                     /locus_tag="IWO45_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0333876.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1521..2087)
                     /locus_tag="IWO45_002421"
     CDS             complement(1521..2087)
                     /locus_tag="IWO45_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0333877.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2101..2517)
                     /locus_tag="IWO45_002422"
     CDS             complement(2101..2517)
                     /locus_tag="IWO45_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0333878.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2974..4908)
                     /locus_tag="IWO45_002423"
     CDS             complement(2974..4908)
                     /locus_tag="IWO45_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0333879.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5210..6145
                     /locus_tag="IWO45_002424"
     CDS             5210..6145
                     /locus_tag="IWO45_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0333880.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6235..7338
                     /locus_tag="IWO45_002425"
     CDS             6235..7338
                     /locus_tag="IWO45_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0333881.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7351..8304
                     /gene="menA"
                     /locus_tag="IWO45_002426"
     CDS             7351..8304
                     /gene="menA"
                     /locus_tag="IWO45_002426"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0333882.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9118..10464)
                     /gene="gor"
                     /locus_tag="IWO45_002427"
     CDS             complement(9118..10464)
                     /gene="gor"
                     /locus_tag="IWO45_002427"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0333883.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10598..11839)
                     /locus_tag="IWO45_002428"
     CDS             complement(10598..11839)
                     /locus_tag="IWO45_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0333884.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11857..12780)
                     /locus_tag="IWO45_002429"
     CDS             complement(11857..12780)
                     /locus_tag="IWO45_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0333885.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13001..15493)
                     /locus_tag="IWO45_002430"
     CDS             complement(13001..15493)
                     /locus_tag="IWO45_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0333886.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15500..15967)
                     /locus_tag="IWO45_002431"
     CDS             complement(15500..15967)
                     /locus_tag="IWO45_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0333887.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4725 a         3358 c         2864 g         5153 t
ORIGIN      
        1 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
       61 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      121 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      181 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      241 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      301 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      361 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
      421 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
      481 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
      541 taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct
      601 tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat
      661 gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta
      721 aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga
      781 ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat
      841 ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt
      901 tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga
      961 acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat
     1021 tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat
     1081 tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa
     1141 ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct
     1201 gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga
     1261 gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt
     1321 gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt
     1381 tgatggcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg
     1441 tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa
     1501 aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt
     1561 ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc
     1621 gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac
     1681 aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc
     1741 atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg
     1801 accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa
     1861 atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct
     1921 tgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca
     1981 ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct
     2041 tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt
     2101 ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt
     2161 atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat
     2221 ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt
     2281 ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg
     2341 gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt
     2401 gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat
     2461 aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt
     2521 tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca
     2581 cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta
     2641 attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt
     2701 gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt
     2761 ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga
     2821 ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt
     2881 attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg
     2941 aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg
     3001 tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa
     3061 cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt
     3121 tacccagtaa aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact
     3181 gcatagccat gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac
     3241 catgaatttt tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt
     3301 tgtgaaccag tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact
     3361 caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc
     3421 agctgtggtt gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc
     3481 ccatgtccaa gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact
     3541 tggtttgaac acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa
     3601 accatagaaa atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc
     3661 tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat
     3721 ataataacca gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat
     3781 agctaaaaat ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc
     3841 aatagaaacc ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat
     3901 cgaagccacg atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg
     3961 cggtgtcggt gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt
     4021 tttatcttcg tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc
     4081 tgattctaat ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag
     4141 tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac
     4201 gattggtgca ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc
     4261 agcatcattt cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt
     4321 gatttcgttt ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc
     4381 cccaatcatt tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt
     4441 acgcagttca tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg
     4501 aatccttaca gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt
     4561 accaaagtca ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc
     4621 taatttggtc ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag
     4681 ttgtacattc ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc
     4741 tacacgtccg ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc
     4801 gatcagagta attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga
     4861 aacaccggca tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct
     4921 ccttatagac tatctatata atgaatatat gataattctt ttagtttgat acttattaca
     4981 caatctccat atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt
     5041 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
     5101 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
     5161 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
     5221 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
     5281 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
     5341 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
     5401 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
     5461 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
     5521 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
     5581 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
     5641 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
     5701 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
     5761 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
     5821 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
     5881 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
     5941 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
     6001 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
     6061 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
     6121 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
     6181 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
     6241 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
     6301 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
     6361 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
     6421 ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
     6481 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
     6541 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
     6601 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
     6661 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
     6721 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
     6781 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
     6841 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
     6901 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
     6961 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
     7021 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
     7081 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
     7141 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
     7201 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
     7261 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
     7321 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
     7381 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
     7441 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
     7501 tttgatttag cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa
     7561 gtatataagt atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga
     7621 aacatgattt tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat
     7681 acgggttggc ttttactttt gatgggattt atctgttgtt ttattggagt attctataca
     7741 ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg
     7801 ggccttggaa tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat
     7861 ctgacagtca acttttcatc tggacaattt gatttggtag gaaatctttg ggctatagca
     7921 gcaatcgtat tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat
     7981 aatttgcggg atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc
     8041 ggacgtcctg cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt
     8101 ttaatggggt tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg
     8161 ccaattgtga agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc
     8221 agctattcca tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta
     8281 acaatcatta tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc
     8341 aagtaataag aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat
     8401 taccgcagat gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt
     8461 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     8521 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg
     8581 tcctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
     8641 taataccgaa ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca
     8701 caattttttt cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag
     8761 aactttcttg gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa
     8821 tttatttata caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt
     8881 ttaaacatcg ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc
     8941 ttcgtcagtt acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat
     9001 tatcgaagca atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga
     9061 ttttctggtt ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta
     9121 tcgcatcgtt acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc
     9181 ttctttagtt gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc
     9241 aacaccaata gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat
     9301 ttcgcatttt tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata
     9361 aactttcaat tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt
     9421 tgcaattggt ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc
     9481 tttctttcca ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt
     9541 cagatctaat ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg
     9601 gtatttatct actttgataa accctttttt gtccaattcc acatttgtat tttccagacc
     9661 taatttttcg gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt
     9721 ttcaccattt tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat
     9781 atgatgtgcg taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag
     9841 cattttatca aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc
     9901 taaaccacga aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc
     9961 tctcttaggt aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc
    10021 aggaatggtc attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata
    10081 ggtttcaccg tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc
    10141 ctcgacatga ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt
    10201 ttctcgtctt tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta
    10261 acttgaggca tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt
    10321 aggtacacaa ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac
    10381 ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat
    10441 tacgatgtaa tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct
    10501 ttatttaacg atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg
    10561 cgaagatgct taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat
    10621 ctccaggttt caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc
    10681 ggtcaatgga ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga
    10741 atcctggacc ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg
    10801 tagcaatctg tgttgcctca tcataagata atacttcacc gacaaattta tatactggga
    10861 ttttacgacg ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg
    10921 tagctaattc tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat
    10981 agttttcagg atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag
    11041 atacataatg gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa
    11101 tcaattctgg aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt
    11161 ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc
    11221 catacttcca gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat
    11281 ggttggacaa tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt
    11341 cgatttcggt atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag
    11401 ctaaaacgac acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg
    11461 ctgaagcttg agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga
    11521 tcaaagctgg atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa
    11581 attcccctgc tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct
    11641 tcgcatcatg ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac
    11701 ctgcacggct tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat
    11761 ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca
    11821 attttctttt tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat
    11881 ataaaagaat cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt
    11941 cattttttcc gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc
    12001 gaatagtcga gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca
    12061 tgtacagtaa tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct
    12121 ggcaaaaata tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt
    12181 aggttccgag ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt
    12241 atagtaattc tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag
    12301 ggtttctaca ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc
    12361 acgagcaagt actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct
    12421 cgttactaat gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat
    12481 cgtataaata atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa
    12541 agccaaatat tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat
    12601 attcgctttt ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt
    12661 aaatgaagca ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc
    12721 tgcagcaagc aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat
    12781 aattttctcc tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc
    12841 ttctatttaa agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata
    12901 tattctgtcc aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa
    12961 gtcttactat tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt
    13021 cctttttagg tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt
    13081 ggtcacctaa acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt
    13141 tttgaagtgc gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga
    13201 tcacatcgat tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga
    13261 taatcgcttt gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga
    13321 agacgacagt ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt
    13381 ctaagatacg tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga
    13441 catttttttc ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat
    13501 ttctaaagtc cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca
    13561 gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat
    13621 atggtttttg acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg
    13681 gaggagaccc gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac
    13741 ggatcagggc atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt
    13801 ttccgacacc agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca
    13861 atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa
    13921 cgacacgttc atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca
    13981 actgctgtag agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta
    14041 cacgatcaga ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt
    14101 tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga
    14161 tcgctgcttc cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg
    14221 ttaagtgatc ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg
    14281 ctttatcggg tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta
    14341 cagcttcatc tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa
    14401 gaatcacttc tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct
    14461 ctaatgcaga atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg
    14521 tttgtaattc tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt
    14581 ctgctccacc tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat
    14641 cattatagat ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg
    14701 taccagcaac caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg
    14761 gcacttcacc taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac
    14821 caggttcacc caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc
    14881 gtttcacttc tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg
    14941 tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt
    15001 gtacatttac atttctacga cgattcattc cttttccagc agaatcacca gctagcccca
    15061 ttttttgttt cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc
    15121 gagtagccat gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt
    15181 tttgagaacc agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg
    15241 gtgaatatgg aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat
    15301 gttcaatttc ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag
    15361 caattccatt ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat
    15421 gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa
    15481 aaagttcttc catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc
    15541 ggagaatccg agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa
    15601 ttgcactcaa catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt
    15661 gaataatagc aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct
    15721 caagtaattg ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac
    15781 cacgtttgct ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt
    15841 tgatttgtga aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca
    15901 tttcactatt ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat
    15961 gtgccatctt attcattccc tttgactaat attgacctta attataacga atttgataat
    16021 agaaagaaag aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac
    16081 ggaatatact attccgtatc
//
LOCUS       DACEDD010000069        15313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEDD010000069 DACEDD010000000
VERSION     DACEDD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.069"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWO45_002432"
     CDS             278..787
                     /locus_tag="IWO45_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0333888.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWO45_002433"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWO45_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0333889.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWO45_002434"
     CDS             2322..3302
                     /locus_tag="IWO45_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0333890.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWO45_002435"
     CDS             3424..4842
                     /locus_tag="IWO45_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0333891.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWO45_002436"
     CDS             4913..6382
                     /locus_tag="IWO45_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0333892.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWO45_002437"
     CDS             6392..6859
                     /locus_tag="IWO45_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0333893.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWO45_002438"
     CDS             6895..7767
                     /locus_tag="IWO45_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0333894.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="IWO45_002439"
     CDS             7849..10383
                     /locus_tag="IWO45_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0333895.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="IWO45_002440"
     CDS             10539..11312
                     /locus_tag="IWO45_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0333896.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="IWO45_002441"
     CDS             11305..12096
                     /locus_tag="IWO45_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0333897.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="IWO45_002442"
     CDS             12106..12597
                     /locus_tag="IWO45_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0333898.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="IWO45_002443"
     CDS             12615..13025
                     /locus_tag="IWO45_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0333899.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="IWO45_002444"
     CDS             13029..14429
                     /locus_tag="IWO45_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0333900.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="IWO45_002445"
     CDS             14452..14826
                     /locus_tag="IWO45_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0333901.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4782 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttc
//
LOCUS       DACEDD010000070        15286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEDD010000070 DACEDD010000000
VERSION     DACEDD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.070"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IWO45_002446"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IWO45_002446"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="IWO45_002447"
     CDS             complement(580..1353)
                     /locus_tag="IWO45_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0333902.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="IWO45_002448"
     CDS             1497..1847
                     /locus_tag="IWO45_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0333903.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="IWO45_002449"
     CDS             1828..2544
                     /locus_tag="IWO45_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0333904.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="IWO45_002450"
     CDS             2544..3992
                     /locus_tag="IWO45_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0333905.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="IWO45_002451"
     CDS             complement(4062..4571)
                     /locus_tag="IWO45_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333906.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="IWO45_002452"
     CDS             complement(4561..5286)
                     /locus_tag="IWO45_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333907.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5509..7629)
                     /locus_tag="IWO45_002453"
     CDS             complement(5509..7629)
                     /locus_tag="IWO45_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0333908.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7859..9037
                     /locus_tag="IWO45_002454"
     CDS             7859..9037
                     /locus_tag="IWO45_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0333909.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9053..9811
                     /gene="aroD"
                     /locus_tag="IWO45_002455"
     CDS             9053..9811
                     /gene="aroD"
                     /locus_tag="IWO45_002455"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0333910.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9834..10319
                     /gene="ybaK"
                     /locus_tag="IWO45_002456"
     CDS             9834..10319
                     /gene="ybaK"
                     /locus_tag="IWO45_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0333911.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10390..11643)
                     /locus_tag="IWO45_002457"
     CDS             complement(10390..11643)
                     /locus_tag="IWO45_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0333912.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11760..13109
                     /gene="gdhA"
                     /locus_tag="IWO45_002458"
     CDS             11760..13109
                     /gene="gdhA"
                     /locus_tag="IWO45_002458"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0333913.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13221..14567
                     /locus_tag="IWO45_002459"
     CDS             13221..14567
                     /locus_tag="IWO45_002459"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0333914.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14875..15261)
                     /locus_tag="IWO45_002460"
     CDS             complement(14875..15261)
                     /locus_tag="IWO45_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0333915.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4990 a         2737 c         3104 g         4455 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
     5521 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
     5581 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
     5641 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
     5701 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
     5761 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
     5821 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
     5881 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
     5941 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
     6001 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
     6061 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
     6121 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
     6181 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
     6241 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
     6301 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
     6361 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     6421 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     6481 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     6541 gaatgtacca gcgttcccat ggaataccgc actaatgtaa ccttgtgttt gacttaaaat
     6601 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     6661 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     6721 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     6781 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     6841 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     6901 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     6961 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     7021 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     7081 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     7141 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     7201 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     7261 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     7321 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     7381 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     7441 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     7501 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     7561 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     7621 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     7681 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     7741 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     7801 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     7861 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     7921 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     7981 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     8041 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     8101 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     8161 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     8221 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     8281 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     8341 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     8401 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     8461 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     8521 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     8581 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     8641 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     8701 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     8761 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     8821 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     8881 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     8941 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     9001 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     9061 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     9121 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     9181 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     9241 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     9301 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     9361 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     9421 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     9481 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     9541 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     9601 ggagttgact agacgggcaa atgctttttt ctccatgcct attattacga tgtccatggg
     9661 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     9721 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     9781 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     9841 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     9901 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     9961 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
    10021 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
    10081 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
    10141 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
    10201 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
    10261 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
    10321 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
    10381 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
    10441 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
    10501 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
    10561 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
    10621 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
    10681 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
    10741 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
    10801 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
    10861 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
    10921 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
    10981 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
    11041 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
    11101 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
    11161 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
    11221 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
    11281 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
    11341 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
    11401 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
    11461 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
    11521 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
    11581 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
    11641 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
    11701 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
    11761 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
    11821 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
    11881 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
    11941 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
    12001 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
    12061 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
    12121 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
    12181 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
    12241 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
    12301 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
    12361 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
    12421 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
    12481 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
    12541 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
    12601 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
    12661 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
    12721 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
    12781 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
    12841 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
    12901 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
    12961 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
    13021 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
    13081 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
    13141 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
    13201 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
    13261 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
    13321 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
    13381 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
    13441 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
    13501 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
    13561 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
    13621 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
    13681 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
    13741 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
    13801 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
    13861 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
    13921 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
    13981 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
    14041 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
    14101 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
    14161 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
    14221 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
    14281 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
    14341 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
    14401 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
    14461 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
    14521 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
    14581 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
    14641 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
    14701 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
    14761 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
    14821 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
    14881 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
    14941 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
    15001 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
    15061 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
    15121 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
    15181 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
    15241 caaccctaaa atttttttca tgaaagatct ccttttttat ttttat
//
LOCUS       DACEDD010000071        15217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEDD010000071 DACEDD010000000
VERSION     DACEDD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.071"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(24..722)
                     /locus_tag="IWO45_002461"
     CDS             complement(24..722)
                     /locus_tag="IWO45_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0333916.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IWO45_002462"
     CDS             complement(715..2067)
                     /locus_tag="IWO45_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0333917.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IWO45_002463"
     CDS             complement(2290..3690)
                     /locus_tag="IWO45_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0333918.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IWO45_002464"
     CDS             3851..4588
                     /locus_tag="IWO45_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0333919.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IWO45_002465"
     CDS             4713..5192
                     /locus_tag="IWO45_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0333920.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IWO45_002466"
     CDS             5189..5956
                     /locus_tag="IWO45_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333921.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IWO45_002467"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IWO45_002467"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0333922.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IWO45_002468"
     CDS             complement(6859..7461)
                     /locus_tag="IWO45_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0333923.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IWO45_002469"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IWO45_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IWO45_002470"
     CDS             8142..8726
                     /locus_tag="IWO45_002470"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0333924.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IWO45_002471"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IWO45_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0333925.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IWO45_002472"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IWO45_002472"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0333926.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IWO45_002473"
     CDS             10627..10806
                     /locus_tag="IWO45_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333927.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IWO45_002474"
     CDS             10828..11853
                     /locus_tag="IWO45_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0333928.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IWO45_002475"
     CDS             12028..13272
                     /locus_tag="IWO45_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0333929.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IWO45_002476"
     CDS             13425..14372
                     /locus_tag="IWO45_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0333930.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IWO45_002477"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IWO45_002477"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0333931.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         5188 a         2720 c         3111 g         4198 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gcttttt
//
LOCUS       DACEDD010000072        14822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEDD010000072 DACEDD010000000
VERSION     DACEDD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.072"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..245
                     /locus_tag="IWO45_002478"
     CDS             6..245
                     /locus_tag="IWO45_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333932.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="IWO45_002479"
     CDS             312..521
                     /locus_tag="IWO45_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333933.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="IWO45_002480"
     CDS             581..1252
                     /locus_tag="IWO45_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0333934.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="IWO45_002481"
     CDS             1305..3281
                     /locus_tag="IWO45_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0333935.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="IWO45_002482"
     CDS             3294..4049
                     /locus_tag="IWO45_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0333936.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="IWO45_002483"
     CDS             4046..4870
                     /locus_tag="IWO45_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333937.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5159..7270
                     /locus_tag="IWO45_002484"
     CDS             5159..7270
                     /locus_tag="IWO45_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0333938.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="IWO45_002485"
     CDS             complement(7308..9365)
                     /locus_tag="IWO45_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0333939.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="IWO45_002486"
     CDS             complement(9479..9691)
                     /locus_tag="IWO45_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333940.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="IWO45_002487"
     CDS             9878..10345
                     /locus_tag="IWO45_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333941.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="IWO45_002488"
     CDS             10342..10584
                     /locus_tag="IWO45_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333942.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="IWO45_002489"
     CDS             10602..10904
                     /locus_tag="IWO45_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333943.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="IWO45_002490"
     CDS             10976..13030
                     /locus_tag="IWO45_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333944.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..>14822
                     /locus_tag="IWO45_002491"
     CDS             13030..>14822
                     /locus_tag="IWO45_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0333945.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHF"
BASE COUNT         5312 a         2295 c         2954 g         4261 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatattttt ccaaggagta gagaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactttcac cgtgagtgac gttgattatc tgttctttca aaatatcaac tcgcccctca
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcaccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtca cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatggtaa ccatatattc ttttggcgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattcggtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatatttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tggacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 at
//
LOCUS       DACEDD010000073        14737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEDD010000073 DACEDD010000000
VERSION     DACEDD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.073"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(547..909)
                     /locus_tag="IWO45_002492"
     CDS             complement(547..909)
                     /locus_tag="IWO45_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333946.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(983..1363)
                     /locus_tag="IWO45_002493"
     CDS             complement(983..1363)
                     /locus_tag="IWO45_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0333947.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1445..2620
                     /locus_tag="IWO45_002494"
     CDS             1445..2620
                     /locus_tag="IWO45_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0333948.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2696..3280)
                     /locus_tag="IWO45_002495"
     CDS             complement(2696..3280)
                     /locus_tag="IWO45_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0333949.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3481..4479
                     /locus_tag="IWO45_002496"
     CDS             3481..4479
                     /locus_tag="IWO45_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0333950.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4591..5100
                     /locus_tag="IWO45_002497"
     CDS             4591..5100
                     /locus_tag="IWO45_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0333951.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5130..5789)
                     /locus_tag="IWO45_002498"
     CDS             complement(5130..5789)
                     /locus_tag="IWO45_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0333952.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5791..6555)
                     /locus_tag="IWO45_002499"
     CDS             complement(5791..6555)
                     /locus_tag="IWO45_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0333953.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6563..7240)
                     /locus_tag="IWO45_002500"
     CDS             complement(6563..7240)
                     /locus_tag="IWO45_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0333954.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7277..8662)
                     /locus_tag="IWO45_002501"
     CDS             complement(7277..8662)
                     /locus_tag="IWO45_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0333955.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8631..8885)
                     /locus_tag="IWO45_002502"
     CDS             complement(8631..8885)
                     /locus_tag="IWO45_002502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333956.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8872..8956)
                     /gene="ffs"
                     /locus_tag="IWO45_002503"
     ncRNA           complement(8872..8956)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO45_002503"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8979..9494)
                     /gene="tadA"
                     /locus_tag="IWO45_002504"
     CDS             complement(8979..9494)
                     /gene="tadA"
                     /locus_tag="IWO45_002504"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0333957.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9601..10458
                     /locus_tag="IWO45_002505"
     CDS             9601..10458
                     /locus_tag="IWO45_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0333958.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10539..10874)
                     /locus_tag="IWO45_002506"
     CDS             complement(10539..10874)
                     /locus_tag="IWO45_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333959.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10941..12311)
                     /locus_tag="IWO45_002507"
     CDS             complement(10941..12311)
                     /locus_tag="IWO45_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0333960.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12389..12925)
                     /locus_tag="IWO45_002508"
     CDS             complement(12389..12925)
                     /locus_tag="IWO45_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0333961.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13234..14715
                     /locus_tag="IWO45_002509"
     CDS             13234..14715
                     /locus_tag="IWO45_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0333962.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4526 a         2925 c         2394 g         4892 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      301 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      361 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      421 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      481 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      541 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      601 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      661 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      721 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      781 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      841 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      901 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      961 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
     1021 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
     1081 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
     1141 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1201 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1261 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1321 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1381 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1441 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1501 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1561 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1621 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1681 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1741 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1801 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1861 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1921 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1981 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     2041 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     2101 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     2161 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2221 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2281 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2341 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2401 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2461 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2521 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2581 acttaatcga atcaagtatg ctttagagac aatcgattaa atatttattc aaaataaaag
     2641 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2701 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2761 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2821 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2881 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2941 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     3001 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     3061 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     3121 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     3181 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     3241 gtcctttttc actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt
     3301 gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt
     3361 ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa
     3421 acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt
     3481 atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt
     3541 tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt
     3601 caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata
     3661 aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc
     3721 catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca
     3781 aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag
     3841 ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc
     3901 gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct
     3961 gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt
     4021 cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac
     4081 ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc
     4141 cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc
     4201 aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga
     4261 aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt
     4321 gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct
     4381 actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac
     4441 agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt
     4501 tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag
     4561 agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa
     4621 aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc
     4681 atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc
     4741 aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat
     4801 atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa
     4861 ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc
     4921 ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac
     4981 agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct
     5041 gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa
     5101 ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc
     5161 aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat
     5221 aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg
     5281 ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga
     5341 tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac
     5401 ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc
     5461 gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa
     5521 aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag
     5581 ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa
     5641 gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg
     5701 atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat
     5761 tccatccact gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt
     5821 cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa
     5881 aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc
     5941 tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat
     6001 gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga
     6061 gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt
     6121 cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata
     6181 ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa
     6241 tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc
     6301 ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg
     6361 agctacatga atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt
     6421 cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg
     6481 tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata
     6541 tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg
     6601 ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact
     6661 ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct
     6721 catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt
     6781 ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa
     6841 gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga
     6901 aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg
     6961 ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg
     7021 acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta
     7081 gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca
     7141 gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt
     7201 tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt
     7261 ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac
     7321 ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa
     7381 atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc agatcgattg
     7441 gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca
     7501 tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt
     7561 tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat
     7621 ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt
     7681 ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt
     7741 ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca
     7801 gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat
     7861 aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt
     7921 tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat
     7981 actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga
     8041 ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt
     8101 ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta
     8161 aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat
     8221 tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg
     8281 ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata
     8341 agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg
     8401 aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca
     8461 gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat
     8521 cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt
     8581 gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat
     8641 gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag
     8701 gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa
     8761 ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt
     8821 acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca
     8881 gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc
     8941 caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag
     9001 tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc
     9061 ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc
     9121 tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa
     9181 acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta
     9241 acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt
     9301 gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt
     9361 ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct
     9421 gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga
     9481 tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg
     9541 ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag
     9601 atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca
     9661 tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat
     9721 tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa
     9781 aaaaaacacc catggtatcg ctatcttttt aaagatgctt cttgtgttca agaaaaaagc
     9841 tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa
     9901 gatactattt tggaattatt tcgctattct gctgatttct ttttttatac agacacagat
     9961 gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg gtactgaaat caacggtatt
    10021 tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat
    10081 tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag
    10141 caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac
    10201 tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa
    10261 caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct
    10321 gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa
    10381 gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg
    10441 cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc
    10501 cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa
    10561 tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag
    10621 attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta
    10681 tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg
    10741 aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat
    10801 acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt
    10861 gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa
    10921 aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact
    10981 gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat
    11041 ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga
    11101 gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta
    11161 ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa
    11221 ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt
    11281 cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct
    11341 tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt
    11401 ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata
    11461 cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat
    11521 tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc
    11581 tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt
    11641 agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat
    11701 tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa
    11761 ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt
    11821 gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc
    11881 ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca
    11941 gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt
    12001 tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca
    12061 gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga
    12121 gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt
    12181 ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat
    12241 gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct
    12301 tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg
    12361 tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca
    12421 agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt
    12481 gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact
    12541 tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg
    12601 aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa
    12661 aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt
    12721 tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga
    12781 aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa
    12841 ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac
    12901 tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata
    12961 aataacataa ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc
    13021 cattaaaaat caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa
    13081 ataaacaata gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg
    13141 cgacactctg tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac
    13201 tatatacaaa aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag
    13261 ttacttgttc tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat
    13321 gacttacata ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc
    13381 aagcaatttt tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc
    13441 aaagaaaccg atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta
    13501 aaagaatcca atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta
    13561 ccaaaaaaat atttacttga acaactttat atatcgccct cctatctcga aaaactgaca
    13621 aaacagctca atcattcctt aaaaaaattc catttacgga tcctatctgt aaaaaattgc
    13681 tgtactcttg aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat
    13741 acatttggag gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac
    13801 agtacagatg agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta
    13861 actttattca tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc
    13921 actgaattga tggacttatt gcagcataca catgattatt cagaatactt tacttctttt
    13981 tcagtgagtc gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc
    14041 tattgttttt ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt
    14101 tccacaagca aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa
    14161 cagtttcctc tcaaccattt agaaaataaa ataccaattt atttttattt tcttgtgttg
    14221 gcagttgttt ttttcgaact gttgggtgaa tccagcagta gtttttctac actttgcttt
    14281 ccagaagcca cgtccaatca ttctagttct tttatacagg aaaagaaatt aagagaaaaa
    14341 atcgatacaa ttttttcaaa aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa
    14401 ttgatccaac acttactaca aacagaaacg aaagagcagt tactcatcca tttgcagata
    14461 accaaacagt ttacaggaaa ttatcatctt caaagccatt tgcagcagtt atttcgcaaa
    14521 gatgctgtta aaattaccgc atattcagaa catgctgatt tgatcattac agatcagcat
    14581 gagaaaattt cagatgaaac ggagactttc tatctggata caacgcacaa tacagtgtta
    14641 tttggcaagt taataaaagc ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa
    14701 acaaagagga tgtgacaaaa ttcttgtcac atcctct
//
LOCUS       DACEDD010000074        14203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEDD010000074 DACEDD010000000
VERSION     DACEDD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.074"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(11..3664)
                     /gene="rpoC"
                     /locus_tag="IWO45_002510"
     CDS             complement(11..3664)
                     /gene="rpoC"
                     /locus_tag="IWO45_002510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0333963.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3743..7360)
                     /gene="rpoB"
                     /locus_tag="IWO45_002511"
     CDS             complement(3743..7360)
                     /gene="rpoB"
                     /locus_tag="IWO45_002511"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0333964.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8088..9056
                     /locus_tag="IWO45_002512"
     CDS             8088..9056
                     /locus_tag="IWO45_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0333965.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9074..9979
                     /locus_tag="IWO45_002513"
     CDS             9074..9979
                     /locus_tag="IWO45_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0333966.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9976..10782
                     /locus_tag="IWO45_002514"
     CDS             9976..10782
                     /locus_tag="IWO45_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0333967.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10939..11907
                     /locus_tag="IWO45_002515"
     CDS             10939..11907
                     /locus_tag="IWO45_002515"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0333968.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12224..12748)
                     /locus_tag="IWO45_002516"
     CDS             complement(12224..12748)
                     /locus_tag="IWO45_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0333969.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13035..>14203
                     /gene="scm"
                     /locus_tag="IWO45_002517"
     CDS             13035..>14203
                     /gene="scm"
                     /locus_tag="IWO45_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0333970.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDST"
BASE COUNT         4026 a         3072 c         2803 g         4302 t
ORIGIN      
        1 tttaaaatat ctattttgtt tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa
       61 tatctgaaat actgtagaca ttttcgcttg ctacaccgac ttcttttggt tccatgtcac
      121 gataacgttg cataccagta ccagctggga tgatcttacc tataataaca ttttctttca
      181 aaccaagaag aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt
      241 cttggaatga tgcagcagat aagaaactat ttgtttctaa tgatgctttt gtgataccta
      301 gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg tagttttgat
      361 ctttaaagtc tgcgatgtct aataatgtac ctggtaagat atctgtatca cctggatcca
      421 ttacacgaac tttacgcagc atttgacgaa ccattacttc gatatgcttg tcgccgattt
      481 ctaccccttg catacggtaa acacgttgta cttcacgaag caagtagttt tcaacagata
      541 atacatcgcg aacttgcaac aattgttttg gatcgattga tccttctgtc aatggtgcac
      601 cacggtggat catatcgcct tctgctactt tcatacgtgc tgtataagga acagtatacg
      661 tacgtgtatc tgtttttcct ttgattgtta cttctttggt acgtgttgca ggatcttctg
      721 agatatcgat gacatcccct gttacttctg tgatcactgc ttgccctttc ggattacgtg
      781 cttcaaagat ttcttggaca cgaggaagac cttgagtgat atcgtctccg gcaaccccac
      841 ctgtatggaa tgtacgcatc gtcaactgag taccaggttc accgattgat tgagcggcga
      901 tcgtaccaac tgcttctccg acttcaacgt cagagccagt tgcaaggttg cgtccgtaac
      961 aatgtttgca gacaccatgt tttgtattac atgtgaagac agaacggatt gtcactgttt
     1021 cgacacctgc atcaacgatt tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa
     1081 tgatcaattc atttgtttca ggatgacgta cttctttacg ggtgtaacgt cccactaaac
     1141 gatcttctaa tgattcgatg atttcatttc cctcgcggat ggcttggatt tcaagacctc
     1201 ggtcagtacc gcaatcttct tcacggatga ttacatcttg ggccacgtca accagacgac
     1261 gagtcaagta acctgaatcg gcagtcttca aggctgtatc ggtcatccct ttacgagccc
     1321 cgtgagttga gataaacatt tccaagacgg atagtccttc acggaagttc gaaatgattg
     1381 gcaattccat gattcgacca ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg
     1441 taaagttgga aatgttacca cgagctccag aatcagacat catgaagatt ggatttttcg
     1501 cttccaaacc ttccatcagt ttttgttgga tgctgtcttt tgcagcattc caaacagcaa
     1561 tgacacgttc ataacgttcg tcatctgtga tcagaccacg acggaattgt tttgtaatag
     1621 tttcaacttg tttatgggca ttttcaatga tttcttgttt ttcattcagt acactgatgt
     1681 cagcgatccc tactgtgata ccggcatgtg tcgaatgttt gtaccctaag tctttcatac
     1741 ggtcaagcat cttagaagtt tctgttactt tgaatcgttt gaagacttct gcgatgatgt
     1801 ttcctaagtt tttcttcttg aacggtaata ctaattcttg ctctttgata tgtgcaggaa
     1861 tatcagtacc agcttcaaca aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg
     1921 ttggttcatt cagataaggg aattcaggag gcatgatctc gttgaagatg atcttaccaa
     1981 ctgttgtgat catcgtacgt tctttttgcc attcagtaaa tggtttttca cctaatgagt
     2041 tagggttcac accgatacgt gaatgtaaat gaacatatcc gttgcgccat gcgataacgg
     2101 cttcgttttg atctctgaag accattcctt cgccttcgcg tccttcttct tccattgtca
     2161 agtagtagtt acctagaacc atatcttgag aaggagtaac aactggttta ccatctttag
     2221 ggttcaagat gttttgagca gctaacatca acatacgtgc ttctgcttgt gcttcttcat
     2281 tcaatggaac gtggacagcc atttggtctc catcgaaatc ggcattataa gcttcacata
     2341 caagtggatg caaacggatt gcgcgtcctt gtactaaaac tggttcgaat gcttggatac
     2401 caagtcggtg aagcgtaggt gcccggttca gcaatactgg gtgttccttg attacgtctt
     2461 ctaaaacatc ccatacttcg tcttcttgac gttcgatttt acgtttcgca tttttgatgt
     2521 ttgaagctag ttcgcgttga acaagctcac gcatcacgaa tggtttgaat aattcaatgg
     2581 ccatttcttt tggaagtcca cattggtaca tcttcaagaa aggtccaaca acgataacgg
     2641 aacgacctga gtagtcgaca cgtttaccta gcaagttttg acggaaacga ccttgtttcc
     2701 ctttcaacat atgagaaaga gatttcaatg ggcggttacc tggtccagta actggacgtc
     2761 cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg cagcatacgt ttttcatttt
     2821 gaacgatgat tccaggtgca ttcaagtcta acaaacgttt caaacggtta ttacggttga
     2881 ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa
     2941 ccattgggcg taaatctggt ggaataacag ggataacgtc cataaccatc cagcttggtt
     3001 gatttcctga agcgcggaat gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt
     3061 tttgtccttg tgcagatttc aattcttctt tcaattcggc tgcttctgca tctagatcaa
     3121 cattatcaag taattgttta atcgcttctg cacccatagc agcttggaat tcttgtccat
     3181 attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg
     3241 tatttcctgg ttcgatgact acatatgaag caaaataaat gatttcttcc aaagcgcgtg
     3301 ggctcatgtc taatacaaga cccatacgtg aaggaatccc tttaaagtac cagatatgtg
     3361 aaacaggagc tgctaattca atatgtccca tgcgttcacg gcgaactttc gaacgagtaa
     3421 cttccactcc gcatcgatca caaacgattc ctttataacg gatacgtttg tattttccgc
     3481 aggcacattc ccagtctttt gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt
     3541 caggttttaa ggtacgatag ttaatcgtct caggtttttt tacttcaccg taagaccagc
     3601 ttctgatttt ttcgggagaa gccaaaccta tttgcatact ttcgaattta tttacatcga
     3661 tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc aagacggctg atcttcgacc
     3721 gccttgcagc tcatagaccg gattagtcta aattatcttc tgcggtttca aagttttgga
     3781 tgacatcgtc tttttcatct tccacttgtt cagcagcttt cttttctaat tctttggcag
     3841 tttgctgttc ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt
     3901 ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa gacacgcatg tccagaccta
     3961 gtgattgcaa ttctttcacc aatacgcgga atgattccgg tacacctggt tttggaattg
     4021 gttcaccctt gacgattgct tcgtatgttt tcacacgtcc gactacgtca tctgatttgt
     4081 atgtcaagat ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc catacttcca
     4141 tttcaccaaa acgttgtcca ccaaattgag ctttacctcc caatggttgt tgtgtaacca
     4201 atgagtaagg tccgattgaa cgtgcatgca atttgtcatc gaccatgtga gcaagtttga
     4261 tcatgtacat cacaccgaca gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt
     4321 acagaacagt tttcgcatcg ctggccatac cggcttcacg aactgtttcc catacgtctt
     4381 catcacttgc accatcaaat actggcgttg ctacgtggat acctaactga cgtgcagcca
     4441 tacccaagtg taattccaat acttgcccga tgttcatacg tgaaggcacc cctaaaggat
     4501 tcagcatgat atcgattggt gttccatcag gtaggaatgg catatcttct tccggcataa
     4561 tacgggatac aaccccttta tttccgtgac gaccggccat tttatcccct tcatggattt
     4621 tacgtttttg aacgatataa acacgaacta acatgtttac gcctggtgac aattcatctc
     4681 cagcttcacg agtaaagatt ttcacatcgt gaacgatacc gccgccgcca tgtggtacac
     4741 gtaatgatgt atcgcggact tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt
     4801 cttctgcaga taattctgtt accccttttg gcgtgacttt ccctacaagc aaatcgccat
     4861 cttggacttc tgcaccaata cggatgatac ccatttcgtc taaatctttt aatgcgtctt
     4921 ccccgacatt agggatttcg cgtgtgattt cttcaggtcc taattttgta tcgcgggctt
     4981 ctgattcata ttcttcaata tggatagaag tgtatacgtc atcttttacc aaacgacggc
     5041 tcatgatgat cgcatcttca tagttgtacc cttcccaagt catgaaacca actaaaacgt
     5101 tttggcctag agccatttcg ccttgttcca tagatggtcc gtctgccaga gtatcccctt
     5161 tttcgacttt ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt ccggagtttg
     5221 aacgacggaa ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga
     5281 tttcagaagc atcaacgtat tcaacgacac cgtcatgttt acataacaaa gcagctcctg
     5341 agtcatgcgc tgatttgtat tccataccag taccaaccca tggtgcttga ggattgatca
     5401 acggcactgc ttgacgctgc atgttggcac ccatcaaggc acggttggag tcatcgtttt
     5461 ccaagaaagg aatacatgct gtcgcaacgg caactacttg tttaggtgat acgtccatgt
     5521 agtctacttt atcaatagat acttctaagt tttcactttg agcacgagcc ataacaacgt
     5581 cttgagcaaa tgttccatct tcattcaatg ggctgtttgc ttgggctacg atgtaatggt
     5641 cttcgatatc agctgtcaag tagtcgattt ggtcagtaac acgacctgtt tcacggtcta
     5701 cacgacgata aggtgtttcg ataaatccga atttatttac tttcgcataa ctggacaaac
     5761 tattgatcaa cccgatgttt gggccttcag gagtttcgat cggacacata cggccatagt
     5821 gggagtagtg aacgtcacga acttcataac cggcgcggtc acgagtcaat ccgccaggtc
     5881 ctaaggctga aagacgacgt ttgtgtgtca attcgccaag agggtttgtt tgatccatga
     5941 actgtgacaa ctgagaagaa ccaaagaatt ctttgatact tgccacaact ggacggatat
     6001 tgatcaattg ttgaggtgtc aatgtttcag tatcttggat cgacattctt tcacgaacca
     6061 cacgttccat acgtgctagc ccgatacgga attggttttg taaaagttca ccgactgaac
     6121 ggatacgacg atttcctaag tggtcgatat catcgacatt gccgatacct tccatcaagt
     6181 tcaagaaata gctcattgaa gcaatgatgt cggctggacg aaccgttttt acagctgctt
     6241 ctggatagcc gtttccgatc acgtttactt cacgttcagg gtctcttggt gaaaatactt
     6301 tgatgacttg gacagtcatt ggatctgtga ccacaccgtc ttctgatgga tagtaagtca
     6361 ctgaatttag gccattatca atgaaaggag ctagtgtttc catgacttgg tgagtcagaa
     6421 ctgttccttt ttcaacgatg atctcgcctg tttctggatc aactaatgtt tcagctaatg
     6481 tcagattcaa tagacgtgtt ttcaaatcta attttttgtt tactttgtaa cgaccaacgt
     6541 ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt
     6601 ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg
     6661 ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc
     6721 tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa tcctaacgca cgaactaata
     6781 cggttaaagg aatcttacgt gtacggtcga tacgtacata agagatgtct tttgcatcag
     6841 tttccatttc tagccaagct ccacggttag ggataacagt agaaccgaat ccttcttttc
     6901 cgtttttgtc gacttttcca tggaaataga cacctggtga acggactaac tgagaaacga
     6961 ttactcgctc tgccccgttg atgatgaatg ttccttgttc tgtcattaat gggaaatcac
     7021 cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta
     7081 catgtaatgg tgcagagtag tttgcatcat gcgcacgagc ttctgctact gtatactttg
     7141 gttctttcaa ttcataatct acaaattcca aagatagatt gccattgaag tcatcgattg
     7201 gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa gaaccattgg taagagtctg
     7261 tttgaatttc gatcaaattt ggtaattcca acacttcact gattcttgcg aaacttctac
     7321 gttcgcgatg ttttccgtat tttactacgt gtccagccaa gctcttcacc cctctaaaat
     7381 gtcattcaaa aagactaccc gatcaattgt tacaataata tttcgaatat tatttttagg
     7441 taacaacaga cattttttgc ggcttttagg caaaaaaaaa ccaaaaatag aacattctct
     7501 tgaaacctta cttcgtgagc ttctactttt gtttccttat attaaagccg aaacggacaa
     7561 tatttcgttt ctgccttttc ttcatcagat agtttttgca acttcatata ctacacttat
     7621 tttctataaa agtcaacact tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat
     7681 gttttcaatt ttatgttttc aattatctga caattcttaa ttgacagacc tttttatctc
     7741 ggttatactt tagaaaattc attttgtgga ggaacttcta tgcgaaccaa catcaaccaa
     7801 atcgcaccaa cacaactgaa taacttttac tttagctact ttagataggt ttgtattcat
     7861 catgatggat acaaacgtta aacgacagtt tgtatctatg atggctaaag aactttgtat
     7921 tgcagcaaaa agccacgtag acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa
     7981 aagtatcaag cattctactt tttcaattta ttttccgact tttcaatcca tttggacatt
     8041 ttgtctgaat ggattttttg tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata
     8101 taatcatcgg attaggcgct gccctgctac tagctttagg aggttgcgca caaaaagaca
     8161 attccgcaga ggcatcagat ggacaaaaaa caattggcgt attgcaactt gtacaacata
     8221 actcgttaga tgctgcctat aaaggattca aagaaggatt agcagaagaa gggtataaag
     8281 aaggcgataa tctaacaatc gattatcaaa atgcccaaaa caatcaagac aacctgaaaa
     8341 gtatgagtga gaaactcgtc aaagaagatc ctgatttgct acttggaatc gccactccag
     8401 cagcacaatc tttattaaat gaaacaacca ctatcccgat gactgtcaca gcggtcacag
     8461 acttggaaca agcccattta gtcacatcca atgaaaaacc tggcggcaat gtgactggca
     8521 cgacagatat ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc gtgccagacg
     8581 ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa ctctaagatc caagctgatt
     8641 tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca
     8701 caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga atctatattc
     8761 ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa
     8821 aaacacctgt cgttgctggc gcagttgatc aagtcaaaga cggcggtcta gcaacagtag
     8881 gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg
     8941 gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga tttggaactg tatgtcaata
     9001 aagacatggc caaagcatta ggcatcaatc cagacagcat caaagaagct gaataaaaca
     9061 caggaggatt ctcatgacaa atttgctctc tcttttctta tcatctgcat cgcaaggggt
     9121 cttatgggcc ctacttgcga ttggtgtata cgtcactttc cgtatcttgg acattgccga
     9181 cttgactgct gaaggaagct ttcctttagg tgcagggatc gcggcagtaa gtattacaaa
     9241 tggctatcat ccattgacag cttgtcttct aggcttttta gggggtgcgg cagcagggct
     9301 tgtttccgga ctgcttcaca caaaattgaa aattcccgcc cttcttgcag ggatcatcac
     9361 catgactggt ttatattctg tcacatcccg tgtgatgggt gcgccgaaca tctctttatt
     9421 gggtcagcca acgatctttc attgggtcga gtcattcggc ataacaaaag acaacgctgt
     9481 actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt tgttcttacg
     9541 tacagaaaca ggacttgcta ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa
     9601 cgggatcaac acagataata tgaagatcat cggttatatg atttctaatg gatgcattgc
     9661 tctttctggc tccttacttg cgcaaaacaa cggctttgct gatttaaatt ctggtattgg
     9721 aacgatcgta attggacttg cttctattat catcgcagaa gttttgttac gtaaccagcc
     9781 actatttctc cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc
     9841 gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct
     9901 agtgatctgt ctttctttcc cgcagatcca gcaaaaattc catttacctc aaaaaaagaa
     9961 aggcggtagt gtcaaatgaa tgacgtctta acattggaaa atctccatca tacctttgaa
    10021 aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt agatcccggc
    10081 gacttcgtga cgatcatcgg tggaaatggg gctggtaaat ctactttgct gaacagcatc
    10141 gccggtacat tttctgtcga tcagggtcaa attttattga atggaaaaaa tatcacgaaa
    10201 aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc taaactaggg
    10261 acagccgttc gtttaacggt agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa
    10321 cgcggcttct tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga ccagctggtt
    10381 cggctaaatt tggggttgga aaatcgttta tcctcagaaa tcggtttgct gtctggagga
    10441 caacgtcaag ctattacgtt attgatggcc acattacagc gtccagaact gatcttatta
    10501 gatgaacaca ctgcagcgct tgaccctgct acttccaaaa cggtgatgga attgacagaa
    10561 aagctgatca ctgagcagaa tctcactgct tttatggtca ctcatgacat ggaagacgcg
    10621 atccgctatg ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt agatatccag
    10681 caagaggaaa aacagcagct cacagtgccc caattattag cgttgttcaa acaaaacagc
    10741 ggcacggcat taaaagacga tcaagtactt ttatcttcat aatcagaaag aggatgggac
    10801 aaaaaatgaa cgcccaccct cttctttttt ctctttcctt ctttcgtttt tgctttatgt
    10861 atcaaccgtt gtttcttcga aaactatttt gttctctcct tcttcctgat agaatggatg
    10921 ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca
    10981 ccagattttc tttccggaga aaagttggca cagcaactgg aagtttcccg gacaagtatt
    11041 tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg
    11101 tatcgttacc tgccctctga cgtactagat gcaaaagaaa tccagcaaga gctacggtcg
    11161 tttattcctg atttgaaagt acaaacgatg ttgacttctg aatctacgat gaaagatgcc
    11221 aaactggcag cagctcaagg agattctgtt tcgaagttat ttgttgctga tacacaagaa
    11281 caagctcgtg gacgttttgg acggcctttt ttcgctgaac ctggacgagg aatctacatg
    11341 agtctcttgc tccatccaga caaacggttt gatgaattgc ctcaatatac gatcatcgca
    11401 gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc gatcaaatgg
    11461 gtcaatgata tctacatgga tggaaaaaaa gtctgtggga ttttatctga agcagtcagt
    11521 gatatggaaa gcggcaggat cagtcatatc atcattggga tcgggcttaa tttttctgtt
    11581 cctcaaaatc attttccagc tgatctccaa gaaaaagcca tctctgtctt tcccgacgga
    11641 caagctagtg tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt gttcacttat
    11701 ctttctcacc cagaaaaagc gttggacttc tataaagaaa aatcattcgt attgggaaaa
    11761 accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat
    11821 catggcgaac tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc
    11881 agtctcacat ctatccgtac gcaataatta tcagatttca caaaaggaca cctgatctca
    11941 tacagtgtcc ttttttctga attctcttca aaagactctc cttattctat cagctgacag
    12001 tttctctatc ctctagaaaa aggtattcta aaaatatcta taaagcatgg tacactttct
    12061 ttaaatgaat caatgcaaaa aacaagagag tcatcagttt acacgatcaa aagagatagc
    12121 aggccgcttc ctttttcaat cttatagata gaccatgggt aacgataaaa acaaaaaaga
    12181 acttgcgact tattttgccg taagttcttt tttagctagt gccttaatct cttaatccca
    12241 acaaacgtct cgcatagatc acctgaccag agtgcatagc agcatcatca ataatagaaa
    12301 cgagacgcat tcctcgtgtg actggtggtg tccagttctc gtcaattact tcatctagct
    12361 cggaaggttc cagccttctt aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc
    12421 ctagcagatc ttctatttga ttagcttgga tctgctttgc ttgttccaga ctatgcttcc
    12481 aatcttgttc gttatctgct atcgtcatcg gaaaccggct ttcccagtct tgactatgcc
    12541 aaattggaga agttcctgct aaatcagcaa tctggaaatc taattctcga gcggtatgcc
    12601 aagccagcca agtcattgat ttgatctgcg gggctctatc ttccactgga aaacgattgg
    12661 cctcctcaat agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac
    12721 tctcgcatag catatctaaa taatccattt attttcctcc tttttattta tcctacttac
    12781 tttttcctaa ttctaggata cctacttata tgttactaca cacccttatt ttttacactt
    12841 taacagtcac catcatcaat agtttggaga ttgaaagaaa aagatagtgt cgaactatca
    12901 attgaacaag aagcaacaat atgaacgttt cattaatatt ctttaaataa acaatgattt
    12961 ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa taggaaaatg tttgaaagga
    13021 aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt cctagttgct ttgttgttca
    13081 atatcttttc gtatggtgtg gccgtttcag cggaaacata cattcctaaa gaccctggga
    13141 atatttataa tgatctaact ggtaactatg gcttgttagg aattgctagt caattccacg
    13201 tatttgctaa aaataagaca actgtgaatg cacatacaga cggaaatata gcaacacatg
    13261 aacttgatgc aaaaaataat tttggtacag agcttcatga aggtttattg aaccaagaaa
    13321 taaattatat acaaaaaact gattctttcg ctaattcttc aggcatacct acaggtagca
    13381 caagaatgaa taaatttgtc gttggcagta acgtggatgt agcacttcaa gatggtcatc
    13441 ctacagtcaa tggatccagg cttgatcact taactccaga tgatttttat aaagatcgtg
    13501 ctgggcatga atatattgat ttcgagcagg aatttgctaa attgaatgta gcagcaaatg
    13561 atcttgcaac gatcactcca gctaaaacat atactgctgc agattttcca gacatgaaca
    13621 atcggacaat cgatttaact gatttatcag ataccgggtt tttattggta aatatcgatg
    13681 cagaagtgtt aacgatgaat acgccacttc aaatcattaa tccaaatgat caagtagttg
    13741 tatttaatgt catcaacagt tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg
    13801 atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa tatctcttgg aattttggaa
    13861 atgagatgac tgatttgaca atcagtgctc cttttcaagg aacaatcctt gcgcctaatg
    13921 ctactatccg cattactcaa aaccaagacg gaacgattat aggaacaaat gtgattttag
    13981 atgcagctac caatcgttgg gatcctaacg aaatatttat cagcgacaat ggtacagata
    14041 cgacagacac aagcgacagt acagatacat ctaccacaag tgatagctca gattcatcaa
    14101 cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggat acatcagctt
    14161 caagcgacag tacagatact acctctacca caagtgatag cac
//
LOCUS       DACEDD010000075        13944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEDD010000075 DACEDD010000000
VERSION     DACEDD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.075"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWO45_002518"
     CDS             949..1422
                     /locus_tag="IWO45_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0333971.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWO45_002519"
     CDS             1431..1658
                     /locus_tag="IWO45_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333972.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWO45_002520"
     CDS             2294..2665
                     /locus_tag="IWO45_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333973.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IWO45_002521"
     CDS             complement(2921..3163)
                     /locus_tag="IWO45_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0333974.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IWO45_002522"
     CDS             complement(3195..4097)
                     /locus_tag="IWO45_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0333975.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IWO45_002523"
     CDS             complement(4110..4298)
                     /locus_tag="IWO45_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0333976.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWO45_002524"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWO45_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0333977.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IWO45_002525"
     CDS             complement(4913..5806)
                     /locus_tag="IWO45_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0333978.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IWO45_002526"
     CDS             complement(5884..6822)
                     /locus_tag="IWO45_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0333979.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IWO45_002527"
     CDS             complement(6856..7206)
                     /locus_tag="IWO45_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0333980.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IWO45_002528"
     CDS             complement(7239..8141)
                     /locus_tag="IWO45_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0333981.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IWO45_002529"
     CDS             complement(8134..8991)
                     /locus_tag="IWO45_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0333982.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IWO45_002530"
     CDS             9325..10134
                     /locus_tag="IWO45_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0333983.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IWO45_002531"
     CDS             10174..10671
                     /locus_tag="IWO45_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0333984.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="IWO45_002532"
     tRNA            complement(11056..11129)
                     /locus_tag="IWO45_002532"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="IWO45_002533"
     CDS             11316..11639
                     /locus_tag="IWO45_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333985.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="IWO45_002534"
     CDS             11803..12057
                     /locus_tag="IWO45_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333986.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="IWO45_002535"
     CDS             12127..12372
                     /locus_tag="IWO45_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0333987.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="IWO45_002536"
     CDS             12448..12813
                     /locus_tag="IWO45_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333988.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="IWO45_002537"
     CDS             complement(12874..13437)
                     /locus_tag="IWO45_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333989.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4089 a         2577 c         2304 g         4974 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atga
//
LOCUS       DACEDD010000076        13917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEDD010000076 DACEDD010000000
VERSION     DACEDD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.076"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWO45_002538"
     CDS             131..505
                     /locus_tag="IWO45_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0333990.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWO45_002539"
     CDS             complement(619..1248)
                     /locus_tag="IWO45_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0333991.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWO45_002540"
     tRNA            complement(1382..1454)
                     /locus_tag="IWO45_002540"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWO45_002541"
     tRNA            1668..1741
                     /locus_tag="IWO45_002541"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWO45_002542"
     CDS             1696..1845
                     /locus_tag="IWO45_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0333992.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWO45_002543"
     CDS             2338..3666
                     /locus_tag="IWO45_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0333993.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWO45_002544"
     CDS             3868..4701
                     /locus_tag="IWO45_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0333994.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWO45_002545"
     CDS             4717..5454
                     /locus_tag="IWO45_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0333995.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IWO45_002546"
     CDS             complement(5609..6577)
                     /locus_tag="IWO45_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0333996.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IWO45_002547"
     CDS             6809..7642
                     /locus_tag="IWO45_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0333997.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IWO45_002548"
     CDS             7676..8515
                     /locus_tag="IWO45_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0333998.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IWO45_002549"
     CDS             8541..9101
                     /locus_tag="IWO45_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0333999.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IWO45_002550"
     CDS             9300..11021
                     /locus_tag="IWO45_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0334000.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IWO45_002551"
     CDS             11186..12328
                     /locus_tag="IWO45_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0334001.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IWO45_002552"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IWO45_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0334002.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACEDD010000077        13808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEDD010000077 DACEDD010000000
VERSION     DACEDD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.077"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWO45_002553"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWO45_002553"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0334003.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IWO45_002554"
     CDS             complement(2261..2512)
                     /locus_tag="IWO45_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0334004.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IWO45_002555"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IWO45_002555"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0334005.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IWO45_002556"
     CDS             3462..3680
                     /locus_tag="IWO45_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334006.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="IWO45_002557"
     CDS             complement(3969..4481)
                     /locus_tag="IWO45_002557"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0334007.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IWO45_002558"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IWO45_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0334008.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7368)
                     /locus_tag="IWO45_002559"
     CDS             complement(6934..7368)
                     /locus_tag="IWO45_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0334009.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="IWO45_002560"
     CDS             complement(7378..8166)
                     /locus_tag="IWO45_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0334010.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="IWO45_002561"
     CDS             complement(8163..9104)
                     /locus_tag="IWO45_002561"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0334011.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="IWO45_002562"
     CDS             complement(9281..9868)
                     /locus_tag="IWO45_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0334012.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IWO45_002563"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IWO45_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0334013.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="IWO45_002564"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="IWO45_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0334014.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="IWO45_002565"
     CDS             complement(12785..13720)
                     /locus_tag="IWO45_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0334015.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DACEDD010000078        13737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEDD010000078 DACEDD010000000
VERSION     DACEDD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.078"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(63..1268)
                     /locus_tag="IWO45_002566"
     CDS             complement(63..1268)
                     /locus_tag="IWO45_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0334016.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IWO45_002567"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IWO45_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IWO45_002568"
     CDS             complement(1447..2832)
                     /locus_tag="IWO45_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0334017.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IWO45_002569"
     CDS             complement(2861..3247)
                     /locus_tag="IWO45_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0334018.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IWO45_002570"
     CDS             complement(3263..3577)
                     /locus_tag="IWO45_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0334019.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IWO45_002571"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IWO45_002571"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0334020.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IWO45_002572"
     CDS             4837..6126
                     /locus_tag="IWO45_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0334021.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="IWO45_002573"
     CDS             complement(6165..6770)
                     /locus_tag="IWO45_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0334022.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="IWO45_002574"
     CDS             complement(6966..7430)
                     /locus_tag="IWO45_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0334023.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="IWO45_002575"
     CDS             complement(7452..7772)
                     /locus_tag="IWO45_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0334024.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IWO45_002576"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IWO45_002576"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0334025.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="IWO45_002577"
     CDS             9194..9544
                     /locus_tag="IWO45_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0334026.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="IWO45_002578"
     CDS             9729..10682
                     /locus_tag="IWO45_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0334027.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="IWO45_002579"
     CDS             10695..12482
                     /locus_tag="IWO45_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334028.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="IWO45_002580"
     CDS             12469..13671
                     /locus_tag="IWO45_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334029.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3938 a         2689 c         2520 g         4590 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gagtttt
//
LOCUS       DACEDD010000079        13419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEDD010000079 DACEDD010000000
VERSION     DACEDD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.079"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWO45_002581"
     CDS             complement(<1..914)
                     /locus_tag="IWO45_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0334030.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWO45_002582"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWO45_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0334031.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWO45_002583"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWO45_002583"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0334032.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWO45_002584"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWO45_002584"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0334033.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWO45_002585"
     CDS             4440..5321
                     /locus_tag="IWO45_002585"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0334034.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWO45_002586"
     CDS             5318..6034
                     /locus_tag="IWO45_002586"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0334035.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWO45_002587"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWO45_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0334036.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWO45_002588"
     CDS             6751..7752
                     /locus_tag="IWO45_002588"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0334037.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWO45_002589"
     CDS             7749..9110
                     /locus_tag="IWO45_002589"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0334038.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWO45_002590"
     CDS             9070..10221
                     /locus_tag="IWO45_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0334039.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWO45_002591"
     CDS             10196..11365
                     /locus_tag="IWO45_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0334040.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWO45_002592"
     CDS             11540..12919
                     /locus_tag="IWO45_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0334041.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13419
                     /locus_tag="IWO45_002593"
                     /pseudo
     CDS             13200..13419
                     /locus_tag="IWO45_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4481 a         2340 c         2918 g         3680 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatggttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatatgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caattcttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa accagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcaaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatc
//
LOCUS       DACEDD010000080        13404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEDD010000080 DACEDD010000000
VERSION     DACEDD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.080"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            59..1105
                     /locus_tag="IWO45_002594"
     CDS             59..1105
                     /locus_tag="IWO45_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0334042.1"
                     /translation="MTRPNHIESSKKEAPKTEGQTKNFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1454..2086
                     /locus_tag="IWO45_002595"
     CDS             1454..2086
                     /locus_tag="IWO45_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0334043.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2674..4113
                     /locus_tag="IWO45_002596"
     CDS             2674..4113
                     /locus_tag="IWO45_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0334044.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4408..4626
                     /locus_tag="IWO45_002597"
                     /pseudo
     CDS             4408..4626
                     /locus_tag="IWO45_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4875..5090
                     /locus_tag="IWO45_002598"
     CDS             4875..5090
                     /locus_tag="IWO45_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334045.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5307..5563)
                     /locus_tag="IWO45_002599"
                     /pseudo
     CDS             complement(5307..5563)
                     /locus_tag="IWO45_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5750..6661)
                     /locus_tag="IWO45_002600"
     CDS             complement(5750..6661)
                     /locus_tag="IWO45_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0334046.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6680..7684)
                     /locus_tag="IWO45_002601"
     CDS             complement(6680..7684)
                     /locus_tag="IWO45_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0334047.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7681..9804)
                     /locus_tag="IWO45_002602"
     CDS             complement(7681..9804)
                     /locus_tag="IWO45_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0334048.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9809..12256)
                     /locus_tag="IWO45_002603"
     CDS             complement(9809..12256)
                     /locus_tag="IWO45_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0334049.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12243..12632)
                     /locus_tag="IWO45_002604"
     CDS             complement(12243..12632)
                     /locus_tag="IWO45_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0334050.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12692..13195)
                     /locus_tag="IWO45_002605"
     CDS             complement(12692..13195)
                     /locus_tag="IWO45_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0334051.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13208..13369)
                     /locus_tag="IWO45_002606"
     CDS             complement(13208..13369)
                     /locus_tag="IWO45_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334052.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4100 a         2639 c         2452 g         4213 t
ORIGIN      
        1 ttcaagtgcc gaatcttcaa caacttcaag tagtacaagt gaaataactt cttcgaaagt
       61 gacaagacca aaccatatag agtcttcaaa aaaggaagcg ccaaaaacgg aaggacaaac
      121 aaaaaatttt tcgacaccac agcaaataga atcaacagaa tctttccaat cacaaccaat
      181 actatctggt aatgaagaaa ttcattttga caaaaatgaa tctgtagaaa attttattat
      241 gaaaattggt gagtctgcga gaaaaattgg tcagaaatat gatttatatg cttctgttat
      301 gattgcacaa gcgatcttag aatctgccag tggtcaaagc cagttagctc aggcaccgaa
      361 ttacaattta tttgggataa aaggaactta taatggaaac tttgtcatta tggtaacaaa
      421 cgaagatttg ggtaatggaa cgctttatac gacccaatca aaatttagag tgtatgaaaa
      481 ctatgaggaa agttttgaag attatgccaa attattaact aagggaatta gtggaaataa
      541 agatttttat gcaggagcat taaaagctaa tagtaaaaca tatcgagaag caactaaatt
      601 ccttacagga cggtatgcaa cagatacaca atactatttg aagctaaatg aattaattaa
      661 aacctatgat ttaaccaact atgataaaga agttaaaggt ccacaaatga gtaagactgg
      721 ataccaagtg cctattcata attattcaat aaccagtttg tttggaaatc gtggaaatga
      781 atttcaccga ggacttgata tggcagctac acagggtgag ccgattcatg cagttaaagc
      841 tggtaaggtt gttaaagcag agtttcacta ctcttgggga aattacgtgg tgatacaaca
      901 cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa tacctagtaa aaactggaga
      961 taatgttttg caaggacaaa taattggcta tgtaggaagt actgggaata gtactggccc
     1021 acaccttcat ttggaaattt gcaaagatag tagtctttct cagagcatgt tagttgatcc
     1081 gaaaacctta ttattcggag actaaaaaag gtaatcaatc tgacaaagta acaagaaaat
     1141 ggttggattc ttagaccttt gatcaggata ttgagactgg aattgatatc tcgattacga
     1201 tgagtttgac aaataaaaca agtccattat cgaatatcga gtgtattatt gccgtcttta
     1261 tgctcatatt ttgagcaggt tttactagat gaaaacaatt tatctatctt gaggatttcg
     1321 tgtacgtatc gttcagtctt gtattataaa ttagtcataa aatcagacta agaaaaattg
     1381 aaaatatttt tttctgattt attaaaagtt aattgaaatt atatggtact tctttcttgg
     1441 gagggtaggt gcagtgaagg aaagatatgg tcaccttact gttgttgagg agattactca
     1501 taatggatat aaaaaatatc gttacctatg tgactgtggg aatgaaacga ttgtatatcg
     1561 atcaaactta ctatcaggta gaaccaaaag ttgtggatgt ggaaattata aaaataggca
     1621 taaatataaa aatttgacaa ataaaacttt tggtaagtta gttgcaatca agccaactaa
     1681 actgcgaagt gaaggaactg ttgtttggga atgtcaatgt ctgtgtggga gaaaaaagtt
     1741 agtatcggca aagcgacttc taaaaggtga tgtaaaaagc tgtggatgtt tgaaatcaaa
     1801 aaagcatcac attgaaaata aaaaattcgg attacttact gcaaagtatc ctttggacaa
     1861 taactgttca tggaaaacaa aatgggtttg tcattgtgag tgtggcagta tgtgtgagat
     1921 aagttatagt aacttatttt ttggacatac tcaaagttgt ggatgtttaa aaaggcaaga
     1981 aacaaggaca tgtgttgaag ggacagtaat agagtgtcta acgtcaaaat taccgaaaaa
     2041 taatacaagt ggtgttaagg gagtttatcg ttctaaagag aaatagattg cgtatataac
     2101 tttaaaaaaa aagaggtact atttaggatc gtatgattct attaagagtg cagctcaagc
     2161 tagaaaagat gctgaaaata aattgtttca acctttgatt gacaaatatc gaggtaatca
     2221 ttaattattg tggataataa ttaacagtga tatataaaca tctataatat attattcata
     2281 ctattgatat ttagtataat cctatggaat tgaatatcat agcaatgtat ttgaaaagca
     2341 aattggaaaa caaataggtg caacaccata aatatgaaaa aattgggaaa aagtagaatg
     2401 gaaatttgct tacgtctcac gagatagctc acactattaa tctgaagaac aatgaagtcg
     2461 acttttagaa attgctagaa gaaagtaaag cgaattatca ctagttacta tagagtaagt
     2521 tcaaataaag catctccaac ttacatggtt acatgaggat atcaaatcga aattactcaa
     2581 gcttcctatg gctagagtcg cgagaagttg ttgggcagta gacataggtt atattgttct
     2641 tagctacacg atcattagaa aggaaatcct attatgtata gttatcaaga agatgcaaaa
     2701 gtccaaaaaa atcgctggtg gattttgatt gctatatcta tgtttacctt catgtccaca
     2761 ttggatggca gtattgtcaa tattgcacta cctaccatct cagatgatat gggcatacca
     2821 atgaatcaag cggaatgggt agtgtccgtt tatctgatga ttgtctgttc ttgtttgttg
     2881 ctgtttggta aaattggcga tagccgtggg aaaatcaaag tatttcggat tggtacgctg
     2941 atctttgtct tgggatcgct gttgtgtggg ttcaatcatt cgctaggtat tttattgttc
     3001 gcacgggtgg tacaagccat tggtgctagt atgactatgg ctacaaatgc cggaatcatc
     3061 acggaagtct ttcccataca tgaacgtggt cgggcattgg gagcaatagg tgcgtttgtt
     3121 tcattaggct cgattgctgg accaggtctt ggcggcttga ttttgtccca attttcttgg
     3181 gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa cgatgttgat tgctgaaaaa
     3241 ttcttaccaa aagatatcac catgagcggc catacaatcg atggaggggg ctttgctgcc
     3301 attgcggcta cgattatcag cttttttgga gcaatctttt tggggcaaga aagtggcttt
     3361 ttccatccag tcccccttgt actgttcgta atttcagtga ttgcctttgt cagcttttta
     3421 aaagttgaaa agaaagcagc ctctccattg atttctttcg gtatttttaa gaacaagatt
     3481 tttaccatga gcttgattac agccttgttg attttttctt ccaacttctt tgtcaatgta
     3541 gtgattcctt tttatctgca aaatgctcgc gggttgagtc ctagctatgc tgggctgttg
     3601 atgatggtct ttcctttgat gatggtcgtc ggggcgcctt taagcggcta cctgaccgac
     3661 cgggttggcc cggagatttt ggtattagta ggcttgggct tattgtcggt aacccaggtc
     3721 atgtatatgt ttttgtcagt ggactctcct ttgtggctat acattgtcgc tactgccatc
     3781 atgggtgccg gcaatgcctt gttccagtcc cccaataata cgatggttat gagctcggtc
     3841 actatcgaaa acttgggact ggcaggctcg atgaattcct ttgcccgaaa tttggggatg
     3901 gtcagcggga tttcgctttc tacgacggtc ttatatcaag cgatgtctgc taagatgggc
     3961 cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct ttttatatgg aatgaagatt
     4021 acctttttag gcagctttgt tttgtgtttt gttgcctttt tcctgactat ctggcgtatc
     4081 aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc actgttgaag aatttttgga
     4141 ggcctgcgta tgggtgagtg ggatttccat ggatgatgga agatgaagtg aacttctaaa
     4201 agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct caatgagatg cttgatttga
     4261 tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta ggtatcccac cgatggacta
     4321 cgttttagag gctactggtt atgaatacgg agccaacgct ctcatctgca taagaataca
     4381 tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa aaatcattaa gatagataaa
     4441 tgatttccgt ctagtcaaat tggttcagaa tgtagacaca cagatggtaa gaagtcactc
     4501 acaattcgag aatggacttg tcctatttgc cgtatacatc ataatcgaaa cgtcaacgat
     4561 agtatcaata ttttgaccaa gggctatgaa tacaagcatt gccttagaaa acatcaagaa
     4621 ccttaggaac aacggggata gcttggttaa taagagatac cgctgtttag gcttgcaagt
     4681 atactctttt atcaagaatt ccgtggcttt agtcatgaga ggcttaatac tatgaaaatt
     4741 ttctttagca tttttgattt aggttccttt tctaattata ataagatgac cactacagta
     4801 acctactgaa atatataaac agccactgta gtaccatttt caaagtagtc tatgagagga
     4861 gaatcttcat tcttatgaac gctggaattg cggatatgaa tttaataaaa aaaaccctta
     4921 atgattttac ttccaattca atatcaaaag gtactggtat caatttaagc acaataaaaa
     4981 agctgaaaag tggtgaaaga tcagtggaaa agctgaatct tttagatgct ataaaaataa
     5041 cggaatttgc tatgaaaaat ggaaaagctg agattgaaat atggagataa ttatcgtcta
     5101 gtgaattact tgccacttag ctgaatttaa cgatccttgg tgcttgaagg gggagattct
     5161 tgggaataga gcatacttgc aagcctatgt ctttactaac aacgatatct cttattaacc
     5221 aagctatccc cgtagttcta aggcttttac ttttatctaa gcaataatcg tattcaaagg
     5281 aaatttttga cccttagaaa gtctcatttt atcagacgtg agcttaggca ttgctgaatt
     5341 ttgttaattc ggttttaggt tgtgctttga gcataatatg aaagtattct ttatcacgat
     5401 ttcattcatc taaggtaatg tgatgtgatt ctgaaattct ttcaaaggca gtttttgcat
     5461 aagctgacat tacatcatca atgacatgtt tacaatgttt cactactaaa ataagggggt
     5521 aaccaagaag aaaaactgaa tgattatttg tatctaattc catggtatat caaccatttc
     5581 ctatatagac tgattatgac atggattaaa ataaaaaggc actatgtctt aattgtcggt
     5641 tttcgagtta ccgaatggca ttcatcaccc acctatagag gatgggcgac ttctgcctga
     5701 aataagttac actacttata acttctaata aaattagatt gagtcattat tatttaacat
     5761 caacgatttt ccagtttcca tttttttgta atactaattt gtactgaaag tagctagtgg
     5821 tttttgatac attatcaagg tattttatct ttgtactcac tagaaaattt tttccgtttt
     5881 gtagataaac tggattctct aactccgaaa actttaagtc aatattgatt ggtcgcatcg
     5941 caccattttc aacataatac tccaattctt tttcagaagc tgatggataa agtttaaaaa
     6001 atgtttctaa aaaatcggtg atttcttccg ttgttttggc atcaatttct gaatcgtttt
     6061 ctacttgttt tgcttcatat tttgccttgt ctggctttcc aatcattgtt ggcaaactag
     6121 ttactaaatg attcctgttt tcatcttcaa agattgtcac tcgatatgca gaagaaattg
     6181 attgcgtctt ttttccctct gtaatatgtt gctccactaa aaatgaaacg acaaattctt
     6241 ccgagtgttt ttcaacatct agaattttta cagattgaac ttcggaactc gtgggaatat
     6301 ctgctcgaac catatcttgg attaaagcga gaccgttttc tgtaaggtac tgttcgagat
     6361 tactcattct cttttcaatt gcttctttgt tatttttcca tgaaaaatat tctttcgcaa
     6421 aatcctctgt aaaactttca acgccagaag tattcactac cttctctttt ataaccactt
     6481 tttcatgagt tgtatgttga tcaatggcgg tgaaattttt ataaacgcca aaagcaagac
     6541 tacaagacaa taaaagccaa agggcaagca ccatttttcg atgcttaccc acggatacaa
     6601 ctcgttcttt tttctttttt gggctctttt ccttttgatt ccgttcaatt tttatcttca
     6661 ttctttttcc tcttttctct tatttgtatc ggccagctcc gataagatgc tgccgccaat
     6721 agttttccgt tagattcgtc caccccactg gatctcctgc atgaaacatg cgattctccc
     6781 ctgcgtaaat gcccacatgg gtcacataag taccggcatc ataggttgaa tgaaagaaaa
     6841 ctaaatcacc tggtttggca tcttttagat caatatgagt catggcatca tattgatcct
     6901 gagccacccg aggaagtttc aggtcaactt tggcataaca ccattgggtc aagccactac
     6961 aatcaaaccc cgtttctgga cttgagccac caaacacata ggccgttcct tcatacttaa
     7021 atgcctcatc aaaaatttct tggactattt tgtcatcaaa ctgagttgta gttaggtatt
     7081 gcttcactaa taaacagtaa aacatattgc cataattata ccgccaccca ccattttcct
     7141 taatcgcgat tgggttgggg taatcgactt tttgaccacc tgatttttcc tttgaaaaag
     7201 attcagctaa agcaaaacta tgcttcttcc cgtttttccc gacatgatct agatacccgc
     7261 caccatagtt ataggcctga ataatactat cttgatcaca ccctagttct ttggaacgct
     7321 taaccaaaga cgcaaaatag agacagcctt gtttgatgga tgcctcggta cttaatgaat
     7381 taggtggtaa tccggctgac tcagaacttt gcataacgtc ttccattgtg ccaccagatt
     7441 ccacttgaat aatcgccaaa agaattggaa tatactctgt tatatccgat tctttggcat
     7501 acttttccac cattgcacgg tgtttcaata cttcttctga gacactagtt ccgccaggag
     7561 aagtgccaga actaccattt ccctcactgt cttcaccaaa caacaaaccc acacacatca
     7621 aaatacccat aaaagagatg gtcagaaaag ttaatgaaat aacacccact ttcttcaagt
     7681 tcattttttc tcacctgacc ttgattttgg acgatggttc attctttttt gtactttttg
     7741 attggtctgc tttggcagag tggacctctg tttcacttgt tggaaagaat ttttcttact
     7801 tgctgattgg cttttccctt ttgaaatgat ttcttgacta gttagttttc gtttgatttc
     7861 gggattcaga tttgctctca caatccgctt tggtgcttct tttttcagca tcatatcttt
     7921 ctgaggttga gctgctttat ctattggaat attcgccctt ctttttccct gacgatttgt
     7981 tgcttcactc agtatcttgc gtttttcgtc cattttcgca tttcttcgtt gtcgttgttt
     8041 ttctcgcaaa tccactcgat ccgctttttc ttggaacagt ccgcgcttga attcttctgg
     8101 tgcttttttc gacttttcga ttcctttatg gagattgtat ttcaaattcg ttggcgtatt
     8161 ccttacctga tcctttactt gtttagtctt atctttcacc aaagcttggg tatccaaaac
     8221 tttgccgatt tttctcccag ccaaattcat tcgggattgt ttcttattat ttgaaactgg
     8281 attctcctta gttaagtcag atagtatttc gtgatcgttc ggtaatcgtg aattctttcg
     8341 tagagaagaa ccggatggct taaatttgga ttttgattgt tcctttgcca acttatttcc
     8401 gactgctgca ccaccaccaa aagccaaagc tcgtcccaca tttcgattga gttgccgcaa
     8461 ttttcgattc attaacattt gcggttttcg catgacgcgt cttcccattg actggctatc
     8521 attactctgg aggttgaaca tactcataat gtcgcccaat ttgaaataga taccggcaaa
     8581 ggtcacaatt tgcagaaaag caaccatgaa aaatggatag gttgccgaaa tattgaaaaa
     8641 catcgtggag atgctaaatg cagtcgtaat caccaaagta acacccgctc gcatcataat
     8701 cgtattgaat aaacgaataa tcgccttctt tcctaaactt tcataagtcg gcaacatgga
     8761 caataagaaa ctaattggca gaaacatcgc aaagataatg aagagaattt gcgagaacaa
     8821 cataatccct gttagaagaa aaacaaagaa ggaaatgcca atattgaaca ataccaaaaa
     8881 gaccacggtc cctaaacggt tcatggtctt ggtaatactg agattcgcat tgtcattgtc
     8941 ttcaatctct gccttgaccg cttcttcccg atctttgcct ttgttttcat ctggactcac
     9001 agatagaatc ttgttgaccc ggtcttcacc aatttcttct atgttggaat catcaaattg
     9061 cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa ctatcccgaa tcaaatccac
     9121 actatctttt ccttggcttt ctgaattggg tacaacgatc tcggttccaa gggtaagggc
     9181 cgcttcacta acatctgagc taaagtcatt gatttttgtg atataggtag gcgcataagc
     9241 aatgaacgaa cccgatagta gaaaaatcat cacaaaatta atcacggctc gaactgcttt
     9301 cgtggtttct cgttttaata atccggtata cgccacatac acccccagag ctaaaatcat
     9361 caataataga aacccaaaat aaaatccgga ggtctggagt ccattttcag taataccagc
     9421 caatgtttgg atattctttc caatactttc agccgtatcc gaaatgaaat ctaatttgta
     9481 agcctcttga accacataac ccgtggcgtt cgacaaatac aaactcaccg tccaaatgaa
     9541 attagtaatg gcataaagac catacatcac gcttttccct aagccatcac cccaattcca
     9601 aggcaaccaa tcccaagagc tatccacaaa gaaatctagt tgatagttgt tcagtgaata
     9661 tttggaatat agatttcctg cttcaactgt atcatcaact agccctgtcg cctcggctac
     9721 cgtaccaact aaactcaata gaactatcag agtgagtatg ctaataaaaa cgattcccac
     9781 aatccgaaaa agtttctttt tcatcccctc actccattct ctcttgaaca ggtggacggg
     9841 tatcaaaggc atggaagagt tcttcaaaga ctggatgtac ctgaaccacg cctacccgac
     9901 catacaaatc ctgcatcaag cattggccat tttccaaatc tcgtaggcgt ttttgattgc
     9961 tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt cttgatttca tgaatatcgg
    10021 tactgcgaaa cgcaaacttc aacccgatat tattcttcaa gcgttcatcc agtaaatcat
    10081 cactattttg cgtgacaaag taaaccccgg cattcatagc ccgcccagca cgaacgagct
    10141 tattcgataa tgttttccct tgcgctactt gaagaaaact ccacgcctca tctaagtcta
    10201 caattttgaa gatttctcga ttactatgga taaagtccaa ggcaaaagtg gaaatgacga
    10261 ttagcatgga aacactaagc aattccatgg tggtatattc ttcaaaagtg gtttctgcat
    10321 ctggtaaaac caaatccgct acttgaatga tatttaactg cctatctaaa ctaatcgact
    10381 gggtaatcga accatctgag aaaagcaaat gggcaaaatc ataatcggta aaggactcga
    10441 tgtgatccgc gatattttcc gcaactggtg agccttcttt tcgaagctca ttaattactt
    10501 gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat ggccttacgc aacactggaa
    10561 acttctcccc atctcgacta gaaattcctg ttaaaaaggt cagaatatca attgctagac
    10621 tttcagaatc cttctttttc tccataatca cataaggatc aagtaatcct tgattttttg
    10681 gcttactagt taagttgaga atattgattt catccgcaat ttctggcaag gtttctttcc
    10741 agccaccccg ttcccctttt ggatcgacaa tcacagcttg tccaccaaaa aggacggcgt
    10801 aataaactaa gagattgtta ctaaaggact ttccgccacc gagtgaaccg aggaatgccg
    10861 ctgctagcgc attggtaact gaatccttga ccccttgtga ggccaatgcc ggtttcaggt
    10921 aaacattgcg ccccgtatct acgttataac caaagtaaat gccttccaac tcccctaaca
    10981 tctgagtggc gccaaaacca agtccggcga gaaaatcaga cgtaacatac tggatataat
    11041 cattcatgta tcgcttacta gaaggtaaga attcttcatg tagccccatc atatcgccaa
    11101 aaggccggac tagttttaca ttggtatcat cataaaaatc tagcacttca tcacaccggc
    11161 gctttagttc atctactgat tcggcactta ctcgaaccac atagctcagt ttgtacatgg
    11221 attctttgga ctgatccaag gtagtttcca attcatctac ggaatccaat gcatctaaga
    11281 cattggaatt tgtttcattg tcagattgat aggcatggtt gtccaagtct tttaattcct
    11341 ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt cacaatctcc acattcatac
    11401 tagtgtcaat tgggaatgta aattgttgtt gctggtaata gaaaagttct gaggagggaa
    11461 actccatttc ccccacaatt gtattaattg ttaagtacgc tacatatgat tcatgattct
    11521 catgttccat gcataagtac cggggctttt cttcaatcaa acaacgactg ggacgtaata
    11581 agtcgtaacg tttcaccaaa gtttccgact tcagcttttt ctttggtagc tgaaattcat
    11641 attcttcaaa tggtgtaccc ttttcgccat agatatgttc aatcaaatac atcagatcac
    11701 tcggtgtgac tcgtctgact ttaaatcttc gagccaattt actttccatt agtttttcaa
    11761 gcttggtata tcggcgaatt tcttcattgg ataaggaaac aaaatcgccc atcaaatgat
    11821 gattcactcc atagacaaat tcttgaaagc cggagaagaa agatttcttg agattcttta
    11881 gattgacctc ttcatctgtc gcaatcagtt tgaagccaat aaaaaagcga taatcaatct
    11941 gactatcgcc aatcatggat actaatgcct ctgtctgtaa atcaattcgt tgtttcgcta
    12001 cttctgttaa tcgcccggta atttcctttt ttgatcgttc ttgcgtggca cgaatactac
    12061 tttctgtggc aatttgtaag gcatgaatct tgccttcacg attttgcgca atcaattgac
    12121 gaaagttatc gtggacttga tatttctgtt caggtgacaa aaaggagtaa ttgtaaggta
    12181 ttaactcata gtaggcaaaa cattctccct cttgattaaa gacaagatta ttttcaatat
    12241 acttaatcgg gtacatagtt cacactcctt accagagtaa tgggttcgtc ccatcgtttc
    12301 ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa atcgtaagtg atagctaata
    12361 aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg ttttttgcga catgaaccaa
    12421 gtaactgcca ccggtatacc taagtatttt aaaaaggccc cctctatcat agatagtgga
    12481 ggcacatggg caaatacgat gacaaacagc aaggataaaa caaaccaagc catctggtta
    12541 aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat aaatgacctt ttcaactgcc
    12601 caaatactgg tgtagctttt tattttcttc atcaaatcat tcctttaaaa agaaggatag
    12661 tttagaaatc aactaccctt caataatttt gttaatatgg atactcaaaa acacctcgac
    12721 tcgttaccaa ataattgcct ccaatctcca agtctctacc acacgcatca aaatcaatat
    12781 acgtcttcaa gtgttctggt acttctccaa aatcaccagc ttctacaatc aagaaatatg
    12841 ctacatcact catatcatcg caatctggat aatagataat atcatccaca tgctctacca
    12901 tctcctcaaa agaattgaag aaagcatgtt gaatctcaga agcaacttca ccaactggcg
    12961 ttccttcaag ttcctccgct aagccacaca accgattaat ttcctcaatt ggcgtatact
    13021 catcaatctc aaagggaagt tcataatcat gaattgcata ttcctcgtat tcgtcattta
    13081 acccaattcg ttctttcact tcatcaaaat cgacaggtgg ggtaaaccac gcccccacca
    13141 attcaccttc gttatatttc ccgaggttcg caatataaac acgcatctgt tccatcgtgc
    13201 ttccccctta cgcaccgaca attcggttaa acaaactgag taagacatcc ttcaccccag
    13261 aagcattaaa gaccaatccg accgcaatca aagcaatcac caaaaagcca atcagtttgg
    13321 aaaactcccg cttaaatcct aaaaacaaac caatgaccac aattgccagt aagactaagg
    13381 attgggcatt ggttaaaaac catt
//
LOCUS       DACEDD010000081        13380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEDD010000081 DACEDD010000000
VERSION     DACEDD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.081"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1364)
                     /locus_tag="IWO45_002607"
     CDS             complement(45..1364)
                     /locus_tag="IWO45_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0334053.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IWO45_002608"
     CDS             complement(1361..2014)
                     /locus_tag="IWO45_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0334054.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IWO45_002609"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IWO45_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IWO45_002610"
     CDS             complement(2724..3440)
                     /locus_tag="IWO45_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0334055.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="IWO45_002611"
     CDS             complement(3564..3749)
                     /locus_tag="IWO45_002611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334056.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="IWO45_002612"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IWO45_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IWO45_002613"
     CDS             complement(5356..6387)
                     /locus_tag="IWO45_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0334057.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IWO45_002614"
     CDS             complement(6394..7224)
                     /locus_tag="IWO45_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0334058.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IWO45_002615"
     CDS             complement(7221..8027)
                     /locus_tag="IWO45_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0334059.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IWO45_002616"
     CDS             complement(8033..8857)
                     /locus_tag="IWO45_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0334060.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IWO45_002617"
     CDS             complement(8847..9947)
                     /locus_tag="IWO45_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0334061.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IWO45_002618"
     CDS             10125..10910
                     /locus_tag="IWO45_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0334062.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IWO45_002619"
     CDS             10943..11326
                     /locus_tag="IWO45_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0334063.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IWO45_002620"
     CDS             11402..11533
                     /locus_tag="IWO45_002620"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0334064.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IWO45_002621"
     CDS             11502..11738
                     /locus_tag="IWO45_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0334065.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IWO45_002622"
     CDS             complement(11816..12787)
                     /locus_tag="IWO45_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0334066.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4066 a         2918 c         2313 g         4083 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
//
LOCUS       DACEDD010000082        13335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEDD010000082 DACEDD010000000
VERSION     DACEDD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.082"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IWO45_002623"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IWO45_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0334067.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IWO45_002624"
     CDS             complement(738..1460)
                     /locus_tag="IWO45_002624"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0334068.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IWO45_002625"
     CDS             complement(1596..2477)
                     /locus_tag="IWO45_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0334069.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IWO45_002626"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IWO45_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0334070.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IWO45_002627"
     CDS             3717..4196
                     /locus_tag="IWO45_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0334071.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IWO45_002628"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IWO45_002628"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0334072.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IWO45_002629"
     CDS             5497..5625
                     /locus_tag="IWO45_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0334073.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IWO45_002630"
     CDS             5629..6174
                     /locus_tag="IWO45_002630"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0334074.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IWO45_002631"
     CDS             complement(6629..8320)
                     /locus_tag="IWO45_002631"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0334075.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IWO45_002632"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IWO45_002632"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0334076.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IWO45_002633"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IWO45_002633"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0334077.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IWO45_002634"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IWO45_002634"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0334078.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12602..13303
                     /locus_tag="IWO45_002635"
     CDS             12602..13303
                     /locus_tag="IWO45_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0334079.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3753 a         2710 c         2368 g         4504 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact acggtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    12301 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    12361 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    12421 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    12481 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    12541 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    12601 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    12661 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    12721 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    12781 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    12841 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    12901 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    12961 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    13021 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    13081 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    13141 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    13201 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    13261 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    13321 agtgaccctg ataaa
//
LOCUS       DACEDD010000083        13054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEDD010000083 DACEDD010000000
VERSION     DACEDD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.083"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(260..979)
                     /locus_tag="IWO45_002636"
     CDS             complement(260..979)
                     /locus_tag="IWO45_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0334080.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IWO45_002637"
     CDS             complement(1028..2674)
                     /locus_tag="IWO45_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0334081.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IWO45_002638"
     CDS             2853..3443
                     /locus_tag="IWO45_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0334082.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IWO45_002639"
     CDS             3535..5040
                     /locus_tag="IWO45_002639"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0334083.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IWO45_002640"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IWO45_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0334084.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IWO45_002641"
     CDS             complement(6476..6994)
                     /locus_tag="IWO45_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334085.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IWO45_002642"
     CDS             complement(7010..7336)
                     /locus_tag="IWO45_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0334086.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IWO45_002643"
     CDS             complement(7349..9328)
                     /locus_tag="IWO45_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0334087.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IWO45_002644"
     CDS             complement(9349..9663)
                     /locus_tag="IWO45_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0334088.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IWO45_002645"
     CDS             9831..10124
                     /locus_tag="IWO45_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0334089.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IWO45_002646"
     CDS             complement(10202..11314)
                     /locus_tag="IWO45_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0334090.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11515..11691
                     /locus_tag="IWO45_002647"
     CDS             11515..11691
                     /locus_tag="IWO45_002647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334091.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            11707..11880
                     /locus_tag="IWO45_002648"
     CDS             11707..11880
                     /locus_tag="IWO45_002648"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334092.1"
                     /translation="MVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITSLL
                     TEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IWO45_002649"
     CDS             12074..12244
                     /locus_tag="IWO45_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0334093.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>13054)
                     /locus_tag="IWO45_002650"
     CDS             complement(12668..>13054)
                     /locus_tag="IWO45_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0334094.1"
                     /translation="QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQA
                     PTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETI
                     ISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4107 a         2633 c         2221 g         4093 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catccaattc ataccccgtt ggcgcttgag tttctaccag ctgatatgct cctggtgcta
    12961 agccatctat cgccagtttt ccatctgctc ctgtggttag acctgactgc agaacggttc
    13021 cattggcatc ttgtaactca aaaacagctc cttg
//
LOCUS       DACEDD010000084        12738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEDD010000084 DACEDD010000000
VERSION     DACEDD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.084"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO45_002651"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO45_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0334095.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWO45_002652"
     CDS             complement(322..891)
                     /locus_tag="IWO45_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0334096.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWO45_002653"
     CDS             1077..1979
                     /locus_tag="IWO45_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0334097.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO45_002654"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO45_002654"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0334098.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO45_002655"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO45_002655"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0334099.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO45_002656"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO45_002656"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0334100.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWO45_002657"
     CDS             8547..8732
                     /locus_tag="IWO45_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0334101.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWO45_002658"
     CDS             complement(8794..9990)
                     /locus_tag="IWO45_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0334102.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWO45_002659"
     CDS             complement(10160..11596)
                     /locus_tag="IWO45_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0334103.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWO45_002660"
     CDS             11889..12698
                     /locus_tag="IWO45_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0334104.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattt
//
LOCUS       DACEDD010000085        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEDD010000085 DACEDD010000000
VERSION     DACEDD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.085"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO45_002661"
     CDS             6..305
                     /locus_tag="IWO45_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0334105.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO45_002662"
     CDS             378..1739
                     /locus_tag="IWO45_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0334106.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO45_002663"
     CDS             1761..2021
                     /locus_tag="IWO45_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334107.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO45_002664"
     CDS             2035..2787
                     /locus_tag="IWO45_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0334108.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO45_002665"
     CDS             complement(2881..3486)
                     /locus_tag="IWO45_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0334109.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO45_002666"
     CDS             complement(3539..4513)
                     /locus_tag="IWO45_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0334110.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO45_002667"
     CDS             complement(4891..5670)
                     /locus_tag="IWO45_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0334111.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO45_002668"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO45_002668"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0334112.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO45_002669"
     CDS             7161..8558
                     /locus_tag="IWO45_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0334113.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO45_002670"
     CDS             8639..9079
                     /locus_tag="IWO45_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0334114.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO45_002671"
     CDS             9227..9553
                     /locus_tag="IWO45_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0334115.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO45_002672"
     CDS             9554..10303
                     /locus_tag="IWO45_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334116.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWO45_002673"
     CDS             complement(10383..10838)
                     /locus_tag="IWO45_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0334117.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWO45_002674"
     CDS             complement(11265..11540)
                     /locus_tag="IWO45_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334118.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWO45_002675"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWO45_002675"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0334119.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACEDD010000086        12590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEDD010000086 DACEDD010000000
VERSION     DACEDD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.086"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..899
                     /locus_tag="IWO45_002676"
                     /pseudo
     CDS             47..899
                     /locus_tag="IWO45_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            996..2882
                     /locus_tag="IWO45_002677"
     CDS             996..2882
                     /locus_tag="IWO45_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0334120.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFETPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2923..3825)
                     /locus_tag="IWO45_002678"
     CDS             complement(2923..3825)
                     /locus_tag="IWO45_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0334121.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            4055..5458
                     /locus_tag="IWO45_002679"
     CDS             4055..5458
                     /locus_tag="IWO45_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0334122.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            5458..6891
                     /gene="ascB"
                     /locus_tag="IWO45_002680"
     CDS             5458..6891
                     /gene="ascB"
                     /locus_tag="IWO45_002680"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0334123.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7036..7962
                     /gene="trxB"
                     /locus_tag="IWO45_002681"
     CDS             7036..7962
                     /gene="trxB"
                     /locus_tag="IWO45_002681"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0334124.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8176..9903
                     /locus_tag="IWO45_002682"
     CDS             8176..9903
                     /locus_tag="IWO45_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0334125.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            9976..10269
                     /locus_tag="IWO45_002683"
     CDS             9976..10269
                     /locus_tag="IWO45_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0334126.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(10364..11224)
                     /locus_tag="IWO45_002684"
     CDS             complement(10364..11224)
                     /locus_tag="IWO45_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0334127.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            11316..11744
                     /locus_tag="IWO45_002685"
     CDS             11316..11744
                     /locus_tag="IWO45_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0334128.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11906..12082
                     /locus_tag="IWO45_002686"
     CDS             11906..12082
                     /locus_tag="IWO45_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0334129.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12109..12555
                     /locus_tag="IWO45_002687"
     CDS             12109..12555
                     /locus_tag="IWO45_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0334130.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4119 a         2170 c         2775 g         3526 t
ORIGIN      
        1 tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc tgattgagaa
       61 agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat
      121 gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga
      181 caaaaaattt cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc
      241 gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct
      301 acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat
      361 cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact
      421 atttcctaaa gagtatgcca tcgggaaaaa aaacagtgaa gaaaatctcg gataaatacc
      481 acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc aatgcacaag
      541 gcattcaaag ccaacaagac aatacctacc tgttcactat aacaatacag gatattctaa
      601 atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat ttttttcgat
      661 tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg
      721 gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat acgtttggtt
      781 gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg tccacggacg
      841 aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt tgtaaataaa
      901 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
      961 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
     1021 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
     1081 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
     1141 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc atcgggaatc
     1201 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
     1261 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
     1321 aaccattcct tgctatccta gctgctgacg ggatgattcg tggactgaca gcattccttg
     1381 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
     1441 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
     1501 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
     1561 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
     1621 aaacacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
     1681 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
     1741 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
     1801 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
     1861 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
     1921 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
     1981 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
     2041 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
     2101 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
     2161 cattgccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
     2221 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
     2281 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
     2341 ccattgcgat ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcagatg
     2401 aaacagatga tagacagaaa agatcaggta aagaaattgt aaaaacaccg atacaaggac
     2461 aaatcgctcc actgaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
     2521 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatgt
     2581 ccctgtttcc gacgaaacat gcaatcggga tggtttctga agcaggtctg gaattgctga
     2641 ttcatgtcgg attggatact gttcagttgg aagggaaata ttttgaaagc ttggtgcagc
     2701 aaggagaaag agtgaaacaa ggcgatctgt tgcttcgctt cgacaaaaag aaaatcgaag
     2761 aggaaggata cagtttagaa acacctgtga ttgtcatcaa ttacatggat tatttggata
     2821 ttttggaaaa tcaaggggaa acggtcgggc cagacgatgc acttatcacc gcactgacct
     2881 gaacggttag gtgaaagaat ggactaggct taactattct ttttagtcca cttctttatt
     2941 ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat caaatttttg
     3001 tcctacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
     3061 taacacgtct agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata
     3121 cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc
     3181 accgatactg gtcaaagcta aaattggcac atgattttgt ttcaatattt ctgcggtacg
     3241 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccacacatat cacttgtgca
     3301 attgtacgct tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat
     3361 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat
     3421 cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat
     3481 gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt
     3541 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
     3601 aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat
     3661 tttttttgct acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg
     3721 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc
     3781 tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc
     3841 ttgttttttt acgtaaatta taactttttt gaaaatgatt tgtaaacgga tttatgttat
     3901 atagcttaat aaagacattt tttgacaaga tatttacaaa aatccagaag aaaacactaa
     3961 cttttgtaaa tgttactgga aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag
     4021 ataataaaag tgtaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
     4081 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
     4141 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
     4201 gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta
     4261 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
     4321 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
     4381 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtggt
     4441 tttggcaatc gttgaagaca tgaagcgttt atgtccaaat gcttggctga ttaatttcgc
     4501 gaacccaagc gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
     4561 cggattatgc aatgtgccag taatggcgat gatgacagaa ccaacaatgc ttggcaagaa
     4621 aaaagaagag ctgatctaca agtttgccgg tctaaatcac ttccattggc acaaagtagc
     4681 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagacaa
     4741 tggattgccg aaaaatatct ttgacatccc gtttttcaaa gaacaattaa aacaaatgaa
     4801 catgatccct tgtggctacc accgctatta ctaccgtgaa gaagaaatgc tggaacatgc
     4861 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
     4921 gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg
     4981 aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa
     5041 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga
     5101 ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg
     5161 ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
     5221 gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc
     5281 gcttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca
     5341 gcagtattta ccgcaattca aggaaacaat cagccggtta aaagcagaag gaatcgaagt
     5401 ccaagatgaa ctggcaagag aattatcaag caatacacat gtaatggagg aagtatgatg
     5461 ggattcagaa aagatttttt atggggcgga gctacagcag ccaatcaatg cgagggcggt
     5521 tatgatgaag gcggacgtgg gttagcgaac gtagacgttg tgccaatcgg agaagaccgt
     5581 tttcctgtca ttactggaaa aaagaaaatg tttgaatttg atgaaaatca tttttatcca
     5641 gcaaaaattg cgatcgatat gtatcatcat tataaggaag atattgcttt atttgcagaa
     5701 atggggttca agacttatcg gatgtccatt gcttggagtc gtatttttcc aatgggcgat
     5761 gaaaaagagc caaatgaatc tgggttgaaa ttttatgagg atgtctttaa agaatgtcaa
     5821 aagtacggga tcgagccgct tgtgacgatt acacattttg attgtccgat gcatttgatc
     5881 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga
     5941 gtcatcttca atcgctataa aggacttgtc aaatattggt tgacattcaa tgagatcaat
     6001 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaagaagg agaaaatgaa
     6061 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
     6121 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
     6181 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
     6241 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
     6301 gaacgaaatg gcattcagat cgaaatggaa cctggggatg ccgcattgct gaaagaacat
     6361 actgttgatt ttatctcctt ttcttattat tcttctcgag tttcgactac tgacaacgaa
     6421 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctggaagca
     6481 agtgaatggg gttggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
     6541 gatcgttatc aaaaaccact atttattgta gaaaacggct taggtgcagt cgatacgccc
     6601 gatgagaatg gttatgtgga agatgattat cgaatcgact atctggcagc acatatccaa
     6661 gcaatgaaag atgcagtaga attagatgga gtcgatctat taggctatac gacttgggga
     6721 tgtattgatt tagtatcagc tggtacaggt gagatgaaga aaaggtacgg attcatttat
     6781 gtcgatcgtg acaacgaggg aaatggcaca ttagcccgtt cgaagaagaa atcattcaac
     6841 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaatgattg atcaatgatt
     6901 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
     6961 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
     7021 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
     7081 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggcgcccct
     7141 ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc ctggtttcga ttcaatcatg
     7201 ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct
     7261 tatggaatcg tgcaagatat caaggatcac ggcagccaca aagaagttat ttgtgaagat
     7321 aaaacttacg aaggaaaaac agtcattatc gcaacaggct gtatccaccg taaattaggc
     7381 gtacctggcg aggaagaatt tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt
     7441 gcgtttttcc gtaacaaacg tctagtagtg atcggaggag gagactcagc agtagaagaa
     7501 gccatctatt tgacacaatt tgcttctgaa gtagtcatcg tccaccgtag agatgaatta
     7561 cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg aaaaaatttc ctttatgtgg
     7621 gatacagtcg ttgacgaaat cgttgggaat gaaatggtag tgacaggtgt gaaagcacgt
     7681 aatgtcaaaa ccggcaatga gtttgatttg gcagcagacg gagtattcat ctatgttgga
     7741 ctggatccat taactgcacc atttagagaa gcagggttga cgaacgaagc tggctggatt
     7801 ccaacagaca acgaaatgaa gacgaatata ccaggcgttt ttgccatcgg agatgttcgt
     7861 gaaaaagatt tgcgacaaat cactactgct gttggtgatg gtgggatcgc tggacaacaa
     7921 gtgtataaat acttggaatc tttagaagaa acaactgcat aagtttaaaa agagtggaag
     7981 aacggtttca attgttcttt tactcttttt tttcgcgtga aatctactga atccgattta
     8041 tgcgaatact tgcaaccgtg cttgataaaa gaatcctttc ttgatttaag cgttttttgg
     8101 tcctcatact tttatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
     8161 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
     8221 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
     8281 gatgcttttt atgcaccgtt ggaattcggt actgcaggca tgcggggaat cttagggcct
     8341 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact tgcacgtttc
     8401 atggatacac aaggcgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
     8461 catcaatcac cagaatttgc catggaagca gccaaaacat tagcacatca caatatccca
     8521 tcttatgtat tcgaaagtct acgtccaaca ccagagcttt cttttgcagt ccgtcatttg
     8581 aagacgtttg cgggaatcat ggtaactgca tcccacaatc cagcagctta caacggatat
     8641 aaagtctacg gcgaagatgg gggacaaatg cctcctgcag acgcagatgc attgacgaaa
     8701 tatgtccgtg aagtcagcaa tccgttaaaa gttgaagtat tatctgacga agaagctgaa
     8761 catagcggat tgatcacgat catcggagaa gaagtggatg ccgcttatct agaagaaatc
     8821 aaagcagtaa cgattgatca tgaattggtg gaaacaatgg ggaaagattt gaaacttgtg
     8881 tacacaccac ttcacggaac aggaaaaatg ttgggcgaac gtgcgttgaa acaagctgga
     8941 ttcgaacaat tcgtactagt tccagaacaa gctgtcgcag acccagattt cactacagtc
     9001 aaatcaccaa atccagaaga acactctgct tttgagtatg cgatacgact tggcgagaaa
     9061 gaaggcgctg atttgctgat tgcaacagat cctgatgcag accgtttagg tgcagccgtt
     9121 cgtttgccaa atggctccta tgaagtattg accggaaacc aaatcggagc actgtttgct
     9181 caatatattt tggaagcaca ccaatcgcga ggcactttac ctgaaaacgc agcggtattg
     9241 aaatcgatcg tttctagcga attacctaca gcaattgcgg aacactatgg ggctaccatg
     9301 ttcaatgtat tgacaggatt caaattcatt gctgaaaaaa ttcaacaatt tgaagaagat
     9361 cattcccata cttttatgtt cggattcgaa gaaagttacg gttatttagt taaacctttc
     9421 gttcgcgaca aagacgcgat ccaagcattg ctcttattag cagaagtagc agcttactat
     9481 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctac
     9541 tacgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
     9601 gctttgatgg aaaaattccg tgaagaagca ccaacagaat ttgccggagc aaaagtcatc
     9661 caaacagaag acttcaaaga gttgacgaga acagaccgtg aaggaaacgt agaaaaacta
     9721 acgacaccgc catctgatgt attgaagtat acgctggaag aaaatggctg gatcgctatc
     9781 cgtccttcag gaactgagcc gaaaatcaaa ttttacatcg gtgtaaaagg aaactcttca
     9841 gaagacgcag ctgaaaaagt agccgcttat gaagcgatta tccaaaagat gacagaagaa
     9901 taaaaaaact tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag
     9961 ttggaggtga atggattgcc agacacgcaa aaaacaaaag tagagaaagt cagccaacta
    10021 ttcaaaatgt tgagtgaccc cactcgtctc aaaattctgc tgtatctgaa agacggggaa
    10081 caaaatgtga cagcaatcac ccaagcagtg gaaatggaac aatctgctgt atcgcatcaa
    10141 ctgcgtttat taagagaaaa ccatgtcgtc aagtcccatc gtgagggaaa agcgattttg
    10201 tatagtcttg atgattctca cgtactggat attttgaacc agacactcaa acatgtggaa
    10261 catcaataac gataggtgca aatagagaca aactggactg tgtcaacgaa tcttcttcgc
    10321 tgacacagtc cagttttttt taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg
    10381 tggtttcagt tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagcgaag
    10441 ccgtttcttc aatagataaa gatgccacat tgatcactac acatcctagt ttttcataaa
    10501 gatcattcgc aaaatcgagt tcagctctaa ttttttcgat atcggaataa gcagtatcag
    10561 ggtttaatcc gtaagagcgc atccgttctt tacggatatt atttaaaata tctgggttat
    10621 tcgttagtcc cacgattttt ttaggatcga tttcccacag ctgtttggga atatgtgctt
    10681 ggggaatcaa aggtaaatta gcaactttta aatttttatt tgctaaaaac aagctcaaag
    10741 gcgttttgga agttcgggat acgcctagca aaacgacatc tgcttctaaa aaaccacgag
    10801 gatctttccc atcatcgtat tttacagcaa attccattgc tttgatccgt ttgaaatagt
    10861 tctcattcaa taaatggagg gcaccaggtt gccgaattgg cttgatacca gttaaacgtt
    10921 caatttctga tatcggtggc gtcatcacat cgaaactaaa taattgatgt tcttcacaaa
    10981 aagatcgtgt tatctcgatc agttcttgat tgattaatgt atgcaggaca agtgcttttt
    11041 cttctaatgc atcttgcaat gcctgcctta atgttttttc atctgtcacg aacgttctgc
    11101 ggaataggtt gaactcaacg gtaggatact gagccatagt agcttgcgct agttttgtcg
    11161 cagtttctcc agcagagtcg gagatgacaa aaaaagtaac gatttctgta gaatcggaag
    11221 acatggataa aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgcg
    11281 tacttcgtaa tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgatcgaac
    11341 aatcattaga aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt
    11401 taacttattt agtgaagcgt aaccgctacg tatctgctaa agaagttttt gactatatga
    11461 atcaggaatt caaaggggta agttatgata cgatttatcg gaatctccat gattttgcag
    11521 agatcgattt actggaagaa accgatttaa atggagaaat gaagtttcgt tttcattgct
    11581 gtcaggggga aatcggccac caccatcatt ttatctgtac agtgtgcgga acaacgaaag
    11641 aaatccatat gtgtccgatg aattatttcg aagaacagct aggtggatgc acgatcgaag
    11701 ggcatagatt cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata
    11761 acatttgata atttaaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat
    11821 aatatttaca ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt
    11881 ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact
    11941 tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc
    12001 tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc
    12061 tagaaaacgc aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta
    12121 agtagattaa acgatgacat gaaagcagcg atgaaagcga aagataagga atctttacaa
    12181 gtcatccgaa tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg
    12241 acagaagaag aagaactaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg
    12301 catgagtttg aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgta
    12361 atagtcgaaa aatatatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa
    12421 gaagcaatca ctcaaactgg tgcttcatca atgaaagaat ttggcaaagt tatgggtgca
    12481 attatgccta aagtcaaagg aaaagcagat ggaaaccaag tgaatgcgat tgtgaaagaa
    12541 ctattacaat cttaatttta tcatttaaac tgcggacagt gattgtccgc
//
LOCUS       DACEDD010000087        11757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEDD010000087 DACEDD010000000
VERSION     DACEDD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.087"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(258..2333)
                     /locus_tag="IWO45_002688"
     CDS             complement(258..2333)
                     /locus_tag="IWO45_002688"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0334131.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2335..3252)
                     /gene="glyQ"
                     /locus_tag="IWO45_002689"
     CDS             complement(2335..3252)
                     /gene="glyQ"
                     /locus_tag="IWO45_002689"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0334132.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3636..4448)
                     /gene="recO"
                     /locus_tag="IWO45_002690"
     CDS             complement(3636..4448)
                     /gene="recO"
                     /locus_tag="IWO45_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0334133.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4591..5490)
                     /locus_tag="IWO45_002691"
     CDS             complement(4591..5490)
                     /locus_tag="IWO45_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0334134.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5505..5906)
                     /locus_tag="IWO45_002692"
     CDS             complement(5505..5906)
                     /locus_tag="IWO45_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0334135.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5884..6360)
                     /gene="ybeY"
                     /locus_tag="IWO45_002693"
     CDS             complement(5884..6360)
                     /gene="ybeY"
                     /locus_tag="IWO45_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0334136.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6377..8569)
                     /locus_tag="IWO45_002694"
     CDS             complement(6377..8569)
                     /locus_tag="IWO45_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0334137.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8593..9564)
                     /locus_tag="IWO45_002695"
     CDS             complement(8593..9564)
                     /locus_tag="IWO45_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0334138.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10269..11648
                     /gene="brnQ"
                     /locus_tag="IWO45_002696"
     CDS             10269..11648
                     /gene="brnQ"
                     /locus_tag="IWO45_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0334139.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3196 a         2451 c         2138 g         3972 t
ORIGIN      
        1 aattcttgac gcaaccactg ttttcttgac tagggcaatt tacgattaaa aagtaaataa
       61 aacattaata agataacaat taatcgattt tatttttatt tccttaattt tcataggtat
      121 tttcaaaagt tacacaaaaa ataaaataac aaaaaaaatt aacattgaat taataatagt
      181 tatttatatt tataacaaat aaaccagaga aacagcctat tttgtgcctg ttctctggtt
      241 tatttacata ttagaaatta ttttgtgatg atttgatcta aacttgctag tgacaaagcc
      301 atggcagcaa tttgacttaa ttgcgttaat cggttcttac gtactgctgg ttcttctgcc
      361 atgaccatcg tctgatcaaa ataattttcg atcagtgggc gaaggcttac aagctgatca
      421 taattttcgt tgatcgtttg gtcagcaaag cttgttttca ttgcctctac tgctttgtgc
      481 agttctttct ccgcttcgtt ttcgaacagt tcagggtcta cttctgattg tttttcaagc
      541 tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccatcgatgg tttgaagtct
      601 tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa atacttttgt cagatctttt
      661 tgttctgcgt tcaaaacagc ttcgataaca tcatgacgga tattcttcgt tgtcaataat
      721 tggcgcagac ggcctttgat aaagtcgatc acctgttgtt gtcccgctga caagccaatg
      781 ccaaaacgat ccgtatcttt actgataact tcactaatcg tttcttgcag aacagacaaa
      841 ggaaaggccc attttttcga ttcgatgatt cgaacgacac catatgcttg acggcgcaaa
      901 gcatatggat cgtttgagcc acttgggatc atcccaactg caaagaaaga aaagatacta
      961 tctaatttat cagcaatcgc taaaacagaa cctactgtag tctcaggtag atctccttca
     1021 ctggatatag gcatgtaatg ttcacggatt gctgttgcta cggctggttt ctcgccttta
     1081 agcagagcat atttttctcc cataatccct tgtagttcag ggaattctcc taccatgttg
     1141 gttactagat caaatttata gatttcagca gcgcgttgca gatccatcaa ttcttcctca
     1201 gatagtccta cttgttcgcc tatgatgcga gcgatgactt gtacccgttg cattttctca
     1261 tagatgctac cgattttttc atggaaagta acagttttta atttttcaac gtattggtcg
     1321 attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg agcagtcaat
     1381 actttttgat tgcctcgtac cacattttcc aattttaccc gatcgccatt acgagcagaa
     1441 ataaaatgag gaagcaacat accttgatcg ttccgtactt cgaaataacg ttgatgttct
     1501 ttcatagaag tgactagtac ttcttcagga acagaaagat atttttcctc gaagtctcca
     1561 gaaaagacag ttggatattc gactagattg ttgacttctt ctagtaaatc ttcatctaga
     1621 tcaatgatcc agtgattttt ttctgctaat tcgtttgctt gtgtaacgat catttcttgt
     1681 cttttttgcg gtgaagcaat aacaaattgt tcatttaatt tttcttcgta ttcttttgca
     1741 tgcgcaaatg ttacatcttc gcctaagaaa cgatgacctc ttgaagctct gtctgttttg
     1801 acatccaaga tctcaaacgg gattatttcg tcatctaaca atgccacgat ccagtgaata
     1861 gggcggatat attcaaaatc gtattctgcc caatgcatcg ttactgggaa agtgaggctt
     1921 ttaatgacat ctgctaaacc tgtcaatact tcttttgctt ctttgccgtg agtgtatttc
     1981 gttacataga catattccac gccttttaat tctctaaaag tgatagcatc tgtcgtcaga
     2041 ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt ttccatctgc gtccaaagca
     2101 atttttttgg ctggtccttt gacttcttct tcgctgtccg cttgtcgttc tggaatattt
     2161 gttactttga ttgctaaacg gcgcggtgtg gaaaatgttt cgatcgtatc ataagtcaat
     2221 tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct tggtgtgact
     2281 acatgagcag gcatttcttc cagcccgatt tcaagtaata agtcttttgc catcttattc
     2341 agcctccttc acgttttctg gatttgctgt gtttttattc aataaaggga atcctaattt
     2401 ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga tacgggaaag
     2461 ataacctgct cgttctgtta cagatactgc accgcgagca tctaataaat tgaatgtatg
     2521 gctgcatttc aaaatataat catacgctgg atgaacaaga ttttcatcga tacagcgttt
     2581 agcttctttt tcaaaacggt caaaattgct tagtagtaag tcttggtcgc ttacttcaaa
     2641 agaatatttg gaatgttcat attctggttg tttgaagatc tcgccatatt tcacgccatc
     2701 agcccattct aaatcataga cactttcaac ttcttggata taagaagcaa gacgttctaa
     2761 tccataagtg atctcagaag taactggttt acaaggtagt cctccgactt gctggaagta
     2821 cgtgaattgt gtgatctcca tcccatcaag ccatacttcc cagcccacac cggcacaacc
     2881 catcgatggg ttctcccagt tgtcttcaac aaaacgaata tcgtgttcta atggatcaat
     2941 tcctaaaagg cgcaagcttt ctaaataaag ctcttggata ttttcaggtg aaggtttcat
     3001 tacgacttga aattgatgat gctgatacaa acggtttggg ttttctccat aacgcccgtc
     3061 agctggtcgg cgggatggct ctacataagc agcattccat ggttccggtc cgattgcccg
     3121 taagaatgta tatgggctca tcgtacctgc tcctttttct gtatcataag cttgcatcag
     3181 catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt ggacggtcaa
     3241 ttttttactc atctgttttg cccctttcaa tttttaagcg ttgctgtcaa taagaataca
     3301 ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga catagggacg aataatttcg
     3361 cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac tcgcaagtgc
     3421 ctcttcaaaa atcgtttggg tccggcttcc actctcccga actcgctaaa ccacgtgttt
     3481 tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttcttgtttg tataatagat
     3541 cctctcagtg aacgatctat ttaatctacg gttaataata gacggaagtc cctgtatttg
     3601 tcaaatagtt ttgatttttt tgttgtatct ttcatttatt cttctttttc aggtatctct
     3661 ttttcaggca tatttttctc tggcgtcttt aatacgtgtg cccaagactt catttgatca
     3721 atgaactttt tgctttttag atgaatacct acatattcat catacaactg gtcaatgagg
     3781 tcacggattg cctgcttcgt ttcttctttc aagtggattc cactgatctt atcataagaa
     3841 atagccgaaa acattcgaac gaaataaaca gcgcgaggat ctgcatgata acggtgttcg
     3901 tccattggcc agtgtttctc gcatatcaca ccgccatatt tggaagaata gtcaaatttt
     3961 ccttttgttt ccccgcatac gacacagtgc gtccaaattg gcgcgattcc gaagcgttgc
     4021 agtatttgaa cttcaaagat attcgtgatg atttctgcat catctccttg gtccatccgc
     4081 tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat attgatgatc ttcgattgct
     4141 gcatctacta gatttagcaa atacgtacca taaccgctca aaaagatatc ttcttggatg
     4201 ttccgaaacg catggacgtc tttgacactg cttaaaaacg acaaaccttc acttcgaaaa
     4261 ttaccgatat agactgcttc ggtaaagggg agcaaagccg gagctaatgg ctgattttgc
     4321 cgatggattc ctttgatata aaacatcatt ttgccagaag attccgtaaa gattttgact
     4381 aatttatctt tttctttgta attacggcta gataaaatca agcctttcga ttctgcaagc
     4441 tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc cgatccagat
     4501 tatttatgtt acgatcttcc gctgtttttc tcaacgtacg gatttttatc cagtttattt
     4561 ctatacctaa cctataagaa gtccagaata tcagtactcg tcttttcggt agccgaagtc
     4621 ttgcagatag acttttttgt ctcgccagtc tttttggact ttgacccaaa gttctagaaa
     4681 tactttgtct cctaaaagat tttcgatatc ctttcttgct ttcgttccta cttgcttcag
     4741 catttttccg cctttgccga taatgattcc tttttggcta tctctttcga cgatgatcgt
     4801 ggcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa cgactgcgac
     4861 agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca attcagaaac
     4921 gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg ggccctctgg
     4981 catttcactg actaatacat ccattaagcg ttcgacattg tttccttctg tagcagagat
     5041 cggaatcact tgtgcaaaag gcatctgcgt cgaataatct tcgatgattc ccagcaactc
     5101 gtctggatgg atcttatcga ttttattgat aatcagataa actggagaat gcacgttttt
     5161 tagacgttca ataataaaat cgtctccccg gccgcgtttt tgatcagcac tgatcataaa
     5221 taagactgca tccacttctt tcaacgcact gtaagctgct tctaccataa aatctcctag
     5281 acgatgcttt ggtttatgga ttcctggtgt atcgataaaa atcagctgag cttctggtgt
     5341 agtatatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca taatggcaat
     5401 tttttgaccc acgattcggt tcaataatgt ggattttcct acattcgggc ggccgacgat
     5461 tgcgacaaac cctgatttat gttctggcat atacgttctc ctttttaatt aaacaacaaa
     5521 tgatatattt tagggacaaa taaaatcaac ccaatgatga cagcaaaaca agcggtcact
     5581 aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa atgaaaatcc
     5641 gtaaacatgt ccactacatt ttcaaaaaca gtattgatga tctctactat ccaaacgaga
     5701 aaaacagcta aaagcagcca taaccactct atccgatcaa gctggaaaat cacgcccata
     5761 aataaagcaa ggataccgaa cgcaacatgt ttcctcatat tgcgttcttc ctcaaaaacg
     5821 gttttgactc cttggacagc aaattccacg gacatcagga aatgtttgtt tttctctacg
     5881 tttttatctt ttgagtccat aggcatccaa gatctctttc tgtaaaccaa acatttcttt
     5941 ctcatcttct ggtgtcatat ggtcataccc gttgatatgc aagaatccat gtaccgctaa
     6001 aaagccaagt tcccgatcat atgaatggcc gtaagcttct gcttgttcag cagcacgttc
     6061 aatagagatc atgatgtctc ctagtacacg aggcaatgga ttctctccat cttcaaaaat
     6121 cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa ctgatgacgt ctgtcggtgc
     6181 atcttttcca cggtaatcac gattgattac ttggattgcc gcattgtcca tgaaagtaac
     6241 agacatttct gtttcctctg gcagctttaa ataagatgct gcaaattgca gcaagtcatc
     6301 gatttctttc attttctctt ctgagacttg gtttgtttca tccataaagg taatatccat
     6361 tatacatcgc ttctttctat tcttcttgtt cttcctttat cttctcaact tctgttttca
     6421 ttttaggata tttgatccga gaatggaagg tgctacttaa accgctgatc aatgattttt
     6481 ccacttttcg gatttctttc aaggtcagtc cactatcgtc taattgacca tcggaaatac
     6541 gctcttcgat aagattatgg acaaaacttt cgattttttc gctcgtcgga tgatccattg
     6601 cccgtactgc agcctcacaa ctgtcagcaa tattgacgat ccctgcttca cgactttgcg
     6661 gttttggtcc aggataacgg aattgctctt ctgttacttc cggattgcgt tctttcgctt
     6721 tgatgtagaa ataacgcatt agcgtggttc catgatgctg tttgcaaata tcgatcacca
     6781 tttgcggcat attataatct tccaatattt tggctccatc tgtcacatgt ccaaagataa
     6841 tatgtttact gtcttctggc aataagaaat tgtgcgggtt ttccgctcct gaaggcaagt
     6901 tctcaacgaa aaagctagca tgtttgatct tgccgatatc atgataatag caagctactc
     6961 gagttagcaa agaacgtccg ccgatttctg ctacagcgtt agcacttaga ttagctacca
     7021 tcatactatg atgataagta cctggtgctt cttccagcaa ctgtttcaat aaaggatgat
     7081 ttggattgct gagttcattt aatacaatta cactgtcatc attgaccatc aattcaatat
     7141 atgggtgtag accgatcgtc agcaagaatg agaagagtgc accagtgaac ccgcagacga
     7201 gcattaacca agtcgttgca tcaccaaagc tcattccttg atagacgatc aaaatgacat
     7261 ccatcatcac aggaaaaata atgacccaca tggcagcaga taatccttgt tctgagatcc
     7321 gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac ataagacatc aagataatcg
     7381 ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact gcttggaaaa
     7441 taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt ggtgcaaaag
     7501 cagcgggata aaacagcggg atataagtag cttgttccgt ttggaataat tggaaaaatt
     7561 tcatcagcac tatgctgact gacatagctg tcacatagaa aagcacgtat tttgttcttt
     7621 ctcctgcatt ttggaattga agtgaattat aaatcagtac gccgatttga agcaaaacag
     7681 ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg attagtcagt cctagtaatt
     7741 ccaatttttt catagctgaa gcatcgattt gattaccttc acgaacaatg atttctcctt
     7801 ggaagatcat cactggttgt acagcttctc gtgcggattg tttcaattct tctgtctttt
     7861 tctcattcaa tacgtcattc actacgatac cttgatccac taagtagcga atcatttgtt
     7921 gttgtgctgg tgtgacattc aaatattgga tttgctcttc tgcttcttgt ttaaaagctt
     7981 ccaattcact ttcacgaact tgtttgctca tctgttcgtc aatgagcttc agactctcat
     8041 ccctcacagt atggatctca gtttcagtca tttcaaatat cgtcgaataa aaattgttcg
     8101 gtagtttttg ataaaacgca acattttctg cattcacatt ttcaaaattt ttcttcagcg
     8161 cggcgatccg ctcttctgta gtaggctttg ggatattttc atcttccttt gccttatcaa
     8221 tcttttcgtc ataagatttg ttgatggcat cattcgtctt atcgatcaat tcaaatagtt
     8281 gcttgatccg attgttctgg tcatctgcaa gatctttttg gtaagtgtat tctggagtga
     8341 ctgcttctgc tgccagtttt cttttttgtt cggtttcttc cgtgttctcg atcgttttgt
     8401 tcgcacggat actttcttct gctacttggc cttctctgaa atcattggcc ttttgtttga
     8461 cactactaaa tgtaacgccg cagataatga taaaaaacaa gaacagcagg aaaggcaatg
     8521 ctgcctttcc aagtttttct tgtagtttct gcagtgtttt attcaacatg tgcttatcct
     8581 cctaagcaaa gcttattctt gatggagatc tgccttttca taagctttga tgatttcggc
     8641 aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga ttttcttgat
     8701 ttgctttaat gttcgttctg catgaacaag accacttgtt gtccctcgag gcagatcaat
     8761 ctgactcgta tctccattga caatcatttt tgattgataa ccaagacgtg tcaggaacat
     8821 tttcatctga gcaactgtcg tgttctgtgc ttcatccaaa ataacgaatg catcatccaa
     8881 tgtccgcccc cgcatgtaag ctaaaggagc aatttcgatc acgcctcgtt ccatcaatcg
     8941 gttcgtatga tctaatccaa aaatttggta taaagcatca taaacaggac gcagataagg
     9001 atctactttt tcttttagat ccccgggaag aaagcctaga ttttcccctg cttccacagc
     9061 aggccgcgtc aaaatgattt tttgcacttc accttttttc aaggcagcga ttgctaatac
     9121 tactgcaaga aaagtttttc ctgtcccggc tggtcctacg ccaaatacga tatcatgttt
     9181 ggctaccgat tcaacatatt tcttttggcc gctgtttttt acccgtatag gttttccatt
     9241 acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg tgcctttttg
     9301 ccccattttc agtgccgtca cgacatctgg tgtacttaca tggattcctc gtttgatcaa
     9361 ttcctgcaat gtccgaatga ctgaggcagc caaggaaatc tgggattgtt caccaatgat
     9421 ctgaatgatt tctccacgtg tatggatggt tgtttctgtt gtttcttcaa tcagttttat
     9481 atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagccgtca atctaatctc
     9541 taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt cattctttcc
     9601 tattttacca tactcagaag aaactatata gcaaataaga aaaggttcta aaagagttgc
     9661 ttaaccataa gtaccaagaa acgttaaaaa atagctgctt ccattctcta aaggtctcgt
     9721 ttgaataaaa aaagctgatc atgtaagcta tgcataagat agaggacaag tattccgtct
     9781 aacttagcaa gtattctctg ggtatttttc ttgtttcaaa aaaagaaaac aaaatatttg
     9841 aaatattctt gcatttcaaa aaaaacaggt gtattataac gtcaacaaat taatatacag
     9901 caaagcgtcg aggaaaacag taggctattc agcgatttat tcccagtgag tcatgtaagt
     9961 gagaaatgac aataaatcga atagacgaac atcatttccg agtcaatgtc gaaaacgtag
    10021 atcattgtgg ctgcctccat tatcaggcta gcggataact ttatcgaaaa gcgtccgtga
    10081 atgaagaagc ttttttaagc gaaaaaaggt ggtaccgttc tagaatctag agcccttttc
    10141 ccaagtggaa aagggctttt tttatatttt aaggtggtgg ttttcatgga atctgatatt
    10201 agtggtattt ctagtttctg caacgtttat tttgttactc aaaatgataa ttattggagg
    10261 aaaaatttat ggaagaaaca aaaacttatt catggaagca gttattgatg gtcagttcac
    10321 ttatcttcgg gatgttcttc gggtcaggta atctgatctt tcctgtccac ttaggtcaaa
    10381 tggctggatc aaactggatc agcgctggtg taggatttgc catctcaggc gctcttttcc
    10441 cattattagc tatcttagct gtagtagtga caaaaagtga tggtctgtat gacttagcta
    10501 aacctgtcgg aaaatggtat gctgccttgt tcttgatcct tgttcattta acgatcggac
    10561 cattctttgg tacaccgcgg acagcagcaa ctgcttttga aatggctgct aaaccctttt
    10621 tgcctgaaaa atatgattca ttaggtatgt ttttattctc tgctcttttc tttttacttg
    10681 cttatttcct tactgtacat ccaacacgtt tggtcaaata tgtcggaaag tatttaaact
    10741 ccttgtttct tgcattatta gctatcattt ttgtcgtttc tttattccaa cccatgggga
    10801 atctaaacca atctgctgca gattcttatc agacgaacgc tttgacaaat ggcgttttag
    10861 aaggatacaa cacggttgat gcagtagctt tacttgcttt aagtgtcact tttgtccatg
    10921 cagttcgtga tcttggtttt aaagataaaa aagtcacaga attaacagca aaagctggta
    10981 gcctgagcgt tgcgttagaa gtcatgattt attttggtct ggtccttctc ggtgcgatga
    11041 gtttgaataa gatgtcttta tcagaaaatg gcggaactgc actttctcag atcactaatt
    11101 tttatttagg taacttcggt gctagcttct taggggtaat ggtcacatta ggtgtcttca
    11161 ctactgctat gggactcgtt gtttcttttg cacaagattt ccataaactg ttccctaaag
    11221 tcagttatat gacatggctg cggttaacga cttttgtctc ttttgtcgtt gcgaatgccg
    11281 gattggataa tatcatccaa tggtctttac cagtattgat gcttttgtat ccattgtcac
    11341 ttgcgttaat cttactttcc ttaacagcaa aattcttcca aaagagtcca tttgtctatc
    11401 aagtaacgat gttcttttct gccgttccag ccgtattgga tatgttggca aattcgcctg
    11461 cattagtgag tcagcaaact gtcgtagcaa gcttactagg attttatcat cattatgttc
    11521 cttttgctgc attaggtctt ggctgggtcg tcccaacgtt gcttggttac gctggcagct
    11581 tgctctttta ttatgtatat cgcgtatcag gatataaaca agaagcaagt gagttaccag
    11641 aagaataacc aaatcaaaca caatttaaca taagagatat taaacaggaa aaaaaacagc
    11701 tagaagaaag aataagattc tttcttctag ctgtttttta gttctttttt tacgaac
//
LOCUS       DACEDD010000088        11731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEDD010000088 DACEDD010000000
VERSION     DACEDD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.088"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(284..2068)
                     /locus_tag="IWO45_002697"
     CDS             complement(284..2068)
                     /locus_tag="IWO45_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0334140.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(2068..3819)
                     /locus_tag="IWO45_002698"
     CDS             complement(2068..3819)
                     /locus_tag="IWO45_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0334141.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(3961..4185)
                     /locus_tag="IWO45_002699"
     CDS             complement(3961..4185)
                     /locus_tag="IWO45_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0334142.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4436..6022
                     /locus_tag="IWO45_002700"
     CDS             4436..6022
                     /locus_tag="IWO45_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0334143.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6088..7863)
                     /locus_tag="IWO45_002701"
     CDS             complement(6088..7863)
                     /locus_tag="IWO45_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0334144.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8074..8976)
                     /locus_tag="IWO45_002702"
     CDS             complement(8074..8976)
                     /locus_tag="IWO45_002702"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0334145.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            9255..11600
                     /locus_tag="IWO45_002703"
     CDS             9255..11600
                     /locus_tag="IWO45_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0334146.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         3557 a         2403 c         2186 g         3585 t
ORIGIN      
        1 acccaaaaag ccgtccatgg aaccatggga gactcatcag gtttagcttg catcaggcaa
       61 tcactatcca agttgtatcg ttttctttat attatcgaaa gcaaacgctt ttgtcaacgc
      121 tttcttagtg gttattagcc gcttttgact agctgtcgtc gtctttttta aatcgggtga
      181 ctacgccact aacgctcaca gatgaagaat ctgcgaagct ctgttttatt ttcactctag
      241 cgcttagggc aaaatgctac tatgctactg tcacaacctc tttttacaca ccactgcttt
      301 gcagtttgta catatctgca tacaaaccat tttgcgctag aagtgtttca tgattcccac
      361 gctctactat atgacctttg tctaaaacta aaatcaattc tgcatcccga atcgtggaca
      421 gccgatgggc aatcgctatc gttgtccgtc cttgtcgcat ttttgccaat ccttcttgga
      481 tcaatccttc ggtttcagta tcgatatttg ctgttgcttc atccaatact agtattttag
      541 gatctgtcac gattgttcgc gcaaaagtga tcagctgtcg ttgtccacta gaatagctgg
      601 caccgtgttc gatcactttt gagtgatatc cttttggtaa ttcatcgatg aacttgtttg
      661 cttgaacaaa ctcagctgca tggcgaatct gttcgtctgt gatagctgga ttcatcatcc
      721 ggatatttcc tgcaatatct ccataaaaca agaaggcatc ttgcaagacc aatcccattt
      781 tcttacgtaa ctcttccatt ggatattctc gaatgtcatg atcatcaatc aagatttggc
      841 cttcataaaa ttcgtaaaac cgcatcaata cgttgatgat cgagcttttt ccgcttccgg
      901 tgtgccccac taatgcaacg gtctcacccg gattagcgac aaaagagata ttgttcaata
      961 cattgtgctc tccatcataa gaaaagctta cattgcgaaa ctcgatctta cctgaagtaa
     1021 tcgcttgatc tgctccagga ttttgttgcg gtgcatattc ttctgtatcc atgattttca
     1081 aaatacgact accggcaacc atcccatctg taaaggtgct caaaaagtcc atcatttgag
     1141 acattggatt aaaaaattcc tggacatagg tcgtaaatgc atagatcagt ccaacttcta
     1201 cgggagaatg caacgcatcg atcccaaaca aagtcaacgc caacgcaatc gccaatgcat
     1261 agagaaaatt gatgattgga cttaacagaa tcgaattcgt tttgatcatc gcatagcgcg
     1321 tgtctaaata ctcgttgttc acttgttcaa attctttagc taaacgagct tcttggcgaa
     1381 actgctggat aatacgcatg ccggaaatcg attcattcaa tttcgtgttc agctgactta
     1441 atttttcccg catatttcgg taaatacgag aactgaattt ctggtaatac cagatgacga
     1501 ttcctaaaat cggcaagaac accaaagtcc acaaggcaat ttctccattg atttgaaaca
     1561 tggcaacgaa agaagaaacg actgcaaaga tcccagtcaa taccatcaaa aagacatacc
     1621 aaaattcaaa tagcgtttct gtgtcattcg tcactcgaga aacggtcgat cctgccggtg
     1681 tctggtcaaa ataacgcata cctagtgtat gcagcttttc aaataatctt acccgaacat
     1741 actgataggt cttcaatgaa gccatggaat acaaatacca ttggaaaaac caaatgatac
     1801 ttttgactat gactccaaac aagtacacac cagcaaaaaa caaaataact tgtgtcgtag
     1861 ctgactgttt agccagatga tcatccataa aagtttggat gatccttggc aataggacat
     1921 tgatcacaga cagggcaaac gcaaataaga tagcaacgat gaacgttctt ctaaaaggtt
     1981 tggcgaactt catcagtcgt ttgatgatct tcttctgttc tttaagtgga atcgattttg
     2041 tccattccga ttgataattt tcatccatca ggcctcgcct ccttctatct tcgcttctaa
     2101 ttgctgtttt tcccacattc gttcatacca gccacctaat gcaagcagct cgtggtgggt
     2161 tcctcgttca atgattttcc cttgatcgac aacaatgatt tcttttgcat gcatcacgct
     2221 gcttaaacgg tgagctgcaa tgatggttgt tttctctttt cttatttctt ttaaattatg
     2281 caagatagct tcttctgttt tggcatctac tgcagaaagt gcatcatcta agatcagcaa
     2341 ttcaggattt ttgatcaatg ctcgagcaat cgaaatcctc tgtttctgtc cgccagataa
     2401 agagactcca cgttctccaa ctaaggtatc ataacctttt ggcaattcct tgatttcatt
     2461 gttgataaat gctaaagaag ccgcatgctc tacttcttct tctgaaagtt ttggattaga
     2521 aaaacggata ttatctcgaa tggtcatcga aaacaggaag tgatcctgag gcacataacc
     2581 gattgatcct aataaagcat tcaatgaata ttctttgatc gaacgtccgc caaattggat
     2641 tttcccttga taatcatcgt attcacgcat cagtaatttg atgattgtcg tttttcccgc
     2701 accagttttc ccaacaatac ctaatgtttc tccttcttgg atcgcaaatt tgatttgttc
     2761 taatgctggt gattcttctt tcggatagtt aaaagaggaa atagctacat tcagctcgcc
     2821 ttttgccggg gtctgcactg cacccagttg ttccacgata tgcgtctttt catgcaaaag
     2881 ttcattgacg cgatcataag aagcatttcc tctttccaat acgttgaaca atcgaccaat
     2941 cgcaaacatc ggccaaacaa gcataccgat atagctaata aatgagacca tctgcccgat
     3001 tgagatcgtg ccttccaaga catactttcc acctaaaata attgttgcaa cataagacag
     3061 accgataatc aaggtaatga atggatcaaa taaggagtct aaaaaattga ctcgtttgtt
     3121 tttaataatt gcatcttcaa tcttttcttc aaaatccgcg agatcttctt tttcttgtcc
     3181 aaatgtttta atgactttga tcccagtgat actttcttgt gtcttatcat tgatactaga
     3241 aaaagcttct tgcgaatcac gaaaagcatc gtggagtttt gaccccagca ctcgtgaagt
     3301 aacggctagc aaaggcagag gaatcagcgc aatcagtgtt aagcgccaat caataaacaa
     3361 aatcatcgca atgatcgttg ttccgcccgt aatcactgaa tctgcaaagg tcaagattcc
     3421 agctcctgct acattctgga ttgcattcaa atcattcgtt gcatgagcca tcaaatctcc
     3481 agtccgatat ttttggtaaa agagattgtc catttctgta aaatgtttga atagctgctg
     3541 ccgaagttct ctctccaacc gagctgcact tccccaaata ttcgtccgcc agatataacg
     3601 aaaaagatat tgtaagaaag ctgcaaggat caataataag atccaccagg aaatgatctt
     3661 tattgataaa ttcttatcgg cgatctcatc aatgatgatc ccaatcactt ttggaggaac
     3721 taactgtacc aaggccactg cgaataggga gaaaatccca attaaataat gtttcttttc
     3781 ctgacgaaaa aaccaaccta attttctaaa aatcgacatt ttactcctcc tttgtcttta
     3841 tcaagagcat aggattgtct gtgcttgtct tttttgctga atagtctgaa tagtctaaca
     3901 aattacgaaa taaaaaacgg actgaaaaaa tcagcccgca aaaagtcttt gggtcagaac
     3961 ctattttttc ccttggttct tcatttgctg catcatctgg cgaattttct tctcagatgg
     4021 tttttgcccc attgacatca tcattgatcg caacatgtcc tcattaacgg gaggattttt
     4081 ttctaaatag tctttcatat atttacgcgc taggaaaaag ccacctactg cgccgattag
     4141 caaagcaata atagcaataa gtacgacaat acctgttgat accatataaa tctctccttt
     4201 ctatttccga attgctctca atgatttatt ttactaaagt ttattgaaaa aagaaaggcg
     4261 tattctttag tttcatgcaa aaaatagtgg tttttttaga gaagaacaaa atttctttca
     4321 ttaaccagca agacacacca cgaaaaaaaa ttaggcatac cgaatctttt tctttaattt
     4381 tttctttctg aggtgtagaa tgtaatcgta gataaatagg aaaggtggtt ttttcttgaa
     4441 taatcaatcc aacaaacaat ttcataagca tcgacttatt gaacatacaa atggtttatc
     4501 tttagctgaa gtaaatggaa cagtggatgt accaaaggga aaaagttttt ttaaaacact
     4561 tcttgcttac tctggaccag gtgcattagt agcagttggt tatatggatc caggtaactg
     4621 gtccacttcg atcactggtg gacaaaattt ccaatattta ttgatgtcag ttattttgat
     4681 gtctagtcta attgctatgc ttttacagta tatggcagca aaattaggaa tcgtgaccca
     4741 aatggatttg gcccaagcaa tccgagcacg gacaagtaag acattgggtg tcattttatg
     4801 gatcttgact gaattagcca ttatggcaac agacatcgca gaagtcattg gtgcagcaat
     4861 cgccctctac ttactttttg atattccatt ggtgattgcc gtctttatta ctgtattaga
     4921 tgttttcgtt ctcttactac tgacaaaagt cgggtttcgt aagatcgaag cactggttgt
     4981 ctgcttaatt ttcgtcattt tattcgtttt tgtctatcaa gttggcttgt ctaacccaga
     5041 ttggcacagt ttatttgctg gctttatccc taacagtcat acattctcag actcaccggt
     5101 catcggcggc gaaactccgc tgactggagc tttagggatt attggtgcaa cagtcatgcc
     5161 gcataactta tatcttcatt cagcagtttc acaaactcgg aagatcgatc accaaaacga
     5221 agaagatgta gcacaagctg tacgtttttc tacttgggat tctaatatcc agttgtcaat
     5281 ggcttttcta gtcaatgcac tgctattgat catgggtgtc gctgtcttca aaaccggttc
     5341 tgtccaagat ccttcctttt ttggtctgta tgaagcttta tctgatccat cagctatgag
     5401 caatggtatt ttgattgctg tagcaaaatc tggtatcttg tccactttat ttgctgtagc
     5461 acttcttgct tctggccaga actctactat tactggtacg ttgaccggtc aagtcatcat
     5521 ggaaggattc atccatatgc gtatcccgat ttggctaaga agactggtca cacgattgct
     5581 ttctgtgatt cctgtattga tttgtgtttt gtttacaagt acaaaaggaa cgatcgaaga
     5641 acatgccgct ttgaataact taatgaatga atcccaggtg ttcttggctt ttgcgttgcc
     5701 attttcgatg attcctttac tgatgatgac caacagccaa gcggagatgg gtagccgatt
     5761 taaaaattca ttcatcgtaa aacttcttgg ctggttctct gtgattagtc taacttactt
     5821 gaacttacga ggattaccag gacagatcga aagtttcttc ggcgagcaag ccacttccgc
     5881 tcaactagca ttggctgatc acatcgctta tgtaatcatt gttcttgtcc ttctcctgtt
     5941 ggcatggacg attggtgatt tatataaagg aaacaaaaaa tatgaacagc ttttgcagga
     6001 aacggtagaa gaaatcgaat aaaaactgat ttttccacac aaagaccctt gatagttagg
     6061 ttaaaaatct agctaccaag ggtcttttta tttagaagat aaagcttttt tcagatttaa
     6121 tttactgaaa actagcatgc ttgttactac gatgaattgc gttaatggca cgcctgcacc
     6181 agtgtagaca cttaaaaaat agtccttttt ttctggcgca taagaacaaa accatgagac
     6241 accgaaaaga aaaaccaatt gtgtcagtat taatccggtg accacaatca aagaggaact
     6301 ataataggca cgatcagctt ttgcaaatac ggttcctagt atcataagta atatccctgc
     6361 tattgaagca atcagcacgg gaatagccag aacttggtaa atttttatga tgacattcgc
     6421 gacttttgct gattttgtta attggaaatt aggtgttctc ggttcattac tatcgatcca
     6481 attctccatt agcggaaggt ttaataattc ttgagcctgt ttgacttgtt cttgcgttcc
     6541 gaaactataa tttcctcctt gatggaaatc tttatatcgc catgcataag cgtatcctga
     6601 ttgaaggaaa tcgaatacga ttggcagatc actcatcgtc tttccatctc ctgatcccat
     6661 cagatagaac tcttttttct ttttgatggt tcccttgtga taggcttctt ctaattcttg
     6721 gttgactttt ttccaaaaat cattggtttc ttttgcattg ttttggtaat aaccagattc
     6781 gcttaccaca tctcgcaatg cccaaaagat gatatctccg ggtaattctt ttacttttcc
     6841 accttgagtc cacgcgtgag tcgtatataa ttcgtcaata cgggtagaaa cagtatggaa
     6901 tgtaggagat gcggctaatg ctttatttag tgcagcatgt gagacccata tctcactgtt
     6961 ttctttattc aagttcgttt catcatctat tcggatcagt tgtttcatca aacgtccaaa
     7021 ttctccactg gaacggtcat tgatggttgc aataccgtat gttgtctcat ttttatattc
     7081 gatccactga tagacgatgg aaaagccaac tataggaagt aaaaacaatg aaagtttcag
     7141 ccattcccgt ttcgagagaa gaaagagtcg tttttttaac ttctttactt ctttgaaaga
     7201 aaagtcttgc cctagcaaga tctggacgaa actgataccc aaagctacta cagtaaaagg
     7261 aagaagccag atagaatctt cgcgaatgaa ccaaaagaaa ggaaaagcga cagaaagccc
     7321 taaaaaccat ggcagcaatt ttctgctaga accgtatttg cggtaataaa tagctaataa
     7381 acaagcgata atgatctcaa cagccggaat cacgagggca ttacgatatg ttcgagtgga
     7441 atattcatgt gtaaaactaa tcggagaaaa caaaaaataa aaatagatac ttaaaagaag
     7501 atacttgttc ttcacaatcg gtttaaacgc gagtgcaacc aatatactgg caaaaatact
     7561 gaacatgccg aacaaaactg cataagaaag atgcagttta tgtgcaagta ctaaaaataa
     7621 gctgtaggag attcctttgg aaagtgttgt agtcgtatac tcccctagcc atttaccatc
     7681 caaaatattt tgagcatagt gaatcagcag ctgatcatca taggtcgccg acatatctgt
     7741 tccatagatt gctttactat ataaatacaa ccgaataatt gtaaataata cggctatgcc
     7801 aagaataaag tatgtatgtt tcttttttag ttggtcgatc aaggggttta aaatgttcag
     7861 catcttttcg tcctttcaat cacgttttgc tacttgtttg tgaaactttt cgttttttac
     7921 ttttctatca gtataccctg tttgtctgaa tttaaaaaga gggggcaaaa ggagcatggc
     7981 gaaaacgaat gcaaaaagca ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc
     8041 ccaatccttt tttgttgctg tcaggtagaa aacttaaagt gtgacttctt ctgctaataa
     8101 ctcagggaac acttcgggat cgaatgcttg cgctttgaac atggaaattt cttttccata
     8161 tggtgcgtaa gctttctttt tatctttacc gacatatggg gtttctaata ttttcgaaat
     8221 gttgtcaaac attggatcat ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa
     8281 accgatattg gcatgccgat ctttatgaga accttgtgga tttttagaat cattgatatg
     8341 cagtacttta agccgatcta atccgatgac cttatcaaac tcttccatta cacctgaaaa
     8401 atcttctttt atattatatc cggcatcatt cgtatgacat gtatcaaaag taacggataa
     8461 tttttcattc aattttactc catcgatgat ttgcgctaat tcctcaaatg ttcgaccgat
     8521 ttccgtacct ttccctgcca tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa
     8581 aacttcattc aacccctcaa taattctagc aatcccagct tctgcccctg ctcctacatg
     8641 agcgcctggg tgaagggtga tttgagtagc acctaaagct tccgcacgga caatctcttc
     8701 tctcagaaat tgtacagcaa atggaaacat ttctggtttg atcgtatttc ccaaattaat
     8761 gatataaggc gcatgaacga cgatatttgt tagtccgttt tctgccataa atgcttttcc
     8821 ggcatcaata ttcatctctt cgatagcttt tcttctagta ttttgaggag cgcctgtata
     8881 gatcataaag gtcgaagatc cgtaactggc tgcttcttct gccgcgccta acagcatttt
     8941 ctttccgctc atgctgacat gtgagcctaa taacatggat ggattcctcc ttgaaaggca
     9001 attgtttcga acaattcctg tatattgata agatgatgta caaagaaaat ggtttgtttt
     9061 tctttgccct gttctccata gtaactgaaa ctttccctaa agaaaagctt aagaactctt
     9121 tcataaaaaa gatcatttcc tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa
     9181 acagccttgt ctgttttttt gtcctattat ttgctataat tagcgaaata aaaccattag
     9241 ttgaggtgac ccttttgcca cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag
     9301 aaaaatggtt tgttcctaag attattttta gagtgtttca atcattgact gtttttatta
     9361 ctgttcttct tttgttgttc gcagctctag gtatcggaat aggtgctggc tactttgcct
     9421 atcttgtcga agacacacaa ttgccgacta aaaaagaact gcagacagaa ctaggaaaca
     9481 ttacggaaac gtcaaagatt gtttatgcag ataatacaga gatatcaaaa atccagacag
     9541 atctgatgcg gacgactatt tcatctgata agatttcgcc tttattgaaa accgcgatta
     9601 tcagtacaga agatgaatac ttcgataaac atcaaggtta tgtccctaaa gctgttttac
     9661 gagcactagt ttcagaagcg actggcatcg gctcgtctgg aggatccact ttgacacagc
     9721 aattagtcaa acaacagatc ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga
     9781 tcgttctttc tgcacaagta gaaaaacact tttcaaaaaa tgagatcatt actacgtatt
     9841 tgaatgtctc accatttggt cggaataata aggggcaaaa tattgctgga gtccaggaag
     9901 ctgctagagg aatctttggt gtagatgcaa aggatgtgac tctgccacaa gctgcttata
     9961 tcgctggttt gcctcaaagc cctatcacgt atagtccata cacgaataca ggcgcgctaa
    10021 aagaagatct ttctgccggt cttgccagaa aagattttgt tttattcagt atgtaccgtg
    10081 aaggacaaat tacgaaagaa caatacgaag aagcgaaagc ctatgatctt acaaaagact
    10141 ttctcccaca acaaatcgcg gaacagactg acagagaatt cttgtattat accgtgatga
    10201 acgaagcaac aaggattatt gcacaacaat tggcagaaaa agataatgct gatatgtcag
    10261 attcatcggt cagtgaagct tattatcaaa aagcacagca aacgatccaa aacaaaggat
    10321 atacgatcca ttcgacgatc gataaagata tctatgctgc attacaaacc ggcgtagaaa
    10381 attatggcta catgttagat gatggtgctg gtagccaagt ggagactgga aatgtattga
    10441 tggataacag aaccggacgt atctatggct tcgtgggtgg tcggaattac agccagaatc
    10501 aaaataacca cgcctttgat acagaacgtc aagctggttc atccatcaaa ccggttcttg
    10561 tttacggtcc tgcaatcgat atgggacttg tcggatcaga atcccgtgta tcagattatg
    10621 caacgacttg gcaagaagga aaaaatgctg gtgagaaaat cgtcaatgcg accaatgaag
    10681 ggtcgaacac cttccaaacc gtccgtgagt cactggaatg gtccaacaat atcccagctt
    10741 atcacttata tcaagatgtg ttgaattccg gtggatcgaa acaatacgct tacgagcact
    10801 acttggcgaa gatgaactac ccagcaaatg ataattgggg cgttgaatcg gctcctttag
    10861 ggacagtaga tgtaacgacg cttcaacaga caaatggatt ccaggcctta gcaaatggtg
    10921 gagtctacga agaaggctat atcattgatt ccatcacaga taatgcaggt aatgtgattt
    10981 ataaacatga aagtaattct gtccgtattt attccgaagc aacggcatcg atcatgaatg
    11041 acatgatgcg tagtgtgatc aatgaaaaaa tcactacacc attcaaagat gtcatctcaa
    11101 gcttaaacgg aaatctagga aatgcagatt gggtcggaaa aactggttct accaatgaat
    11161 accgagattc atggctgatc gtctcaactc catctatcac tatcagtagt tgggcaggac
    11221 acgatgacaa tactggaatg gacagccaag caagaatacg ttcggctaat tatcttgcca
    11281 atctaatcaa tcaagcttac caagcaaacc ctgagatttt cggcactgat gagaaatttg
    11341 acttatcatc cgatgtgatg aagaaaaaag tatctgggtt cacaggacaa acagctggaa
    11401 aagttactgt caataaaaaa tccatccaaa cgccaggaaa aacagtggag tcactttggg
    11461 caaaaaatgg tccgaagaaa agcgccttta aattcggcgt aggcggtact gatgaaaact
    11521 atacagatta ctggaacacc gcttcagctt atgctagagc gaatccagag accaaagaat
    11581 cagacgataa agatgattga tctaatagat taatcagaat taaagggagc tgcgacaaaa
    11641 atcttgtcac agctcccttt ttccgaataa atggtgttca aaagcttaca tctacgatat
    11701 taagacaaat ttatgtaaaa atgtgaaaaa c
//
LOCUS       DACEDD010000089        11524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEDD010000089 DACEDD010000000
VERSION     DACEDD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.089"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(115..450)
                     /locus_tag="IWO45_002704"
     CDS             complement(115..450)
                     /locus_tag="IWO45_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0334147.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            736..2616
                     /locus_tag="IWO45_002705"
     CDS             736..2616
                     /locus_tag="IWO45_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0334148.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2695..2922
                     /locus_tag="IWO45_002706"
     CDS             2695..2922
                     /locus_tag="IWO45_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ0334149.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2927..3469
                     /locus_tag="IWO45_002707"
     CDS             2927..3469
                     /locus_tag="IWO45_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0334150.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3720..4397
                     /locus_tag="IWO45_002708"
     CDS             3720..4397
                     /locus_tag="IWO45_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0334151.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4718..5959
                     /gene="sstT"
                     /locus_tag="IWO45_002709"
     CDS             4718..5959
                     /gene="sstT"
                     /locus_tag="IWO45_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0334152.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            6106..6741
                     /locus_tag="IWO45_002710"
     CDS             6106..6741
                     /locus_tag="IWO45_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0334153.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6932..7675
                     /gene="truA"
                     /locus_tag="IWO45_002711"
     CDS             6932..7675
                     /gene="truA"
                     /locus_tag="IWO45_002711"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0334154.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7909..8742
                     /gene="msrA"
                     /locus_tag="IWO45_002712"
     CDS             7909..8742
                     /gene="msrA"
                     /locus_tag="IWO45_002712"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0334155.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8823..9816
                     /locus_tag="IWO45_002713"
                     /pseudo
     CDS             8823..9816
                     /locus_tag="IWO45_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            10105..10803
                     /locus_tag="IWO45_002714"
     CDS             10105..10803
                     /locus_tag="IWO45_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0334156.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10953..11408
                     /locus_tag="IWO45_002715"
                     /pseudo
     CDS             10953..11408
                     /locus_tag="IWO45_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT         3807 a         1864 c         2398 g         3455 t
ORIGIN      
        1 gtaaccgata cattagtttc aatgtttact gagtaactaa ttattttgca ggaggacaat
       61 ttatttacac aaaattattg acgctcccaa aatactcgtt tacataaaaa aagattattt
      121 agatgcgata atatcataga taaagaaaca ttcatcataa gaaacagtga tcccaaattc
      181 tgtttcaata tttttgaagg ccttttttac atgaaaaatt gtttcatgag aaggtcttgt
      241 gtcatcttct gatataattt taagttcatg accgtttatc aatgtacgct caattagaca
      301 cgctgtatga atcaaaagtt ttaaaagaat ggaatgagtg aattttttgt ggaaagtaca
      361 ttccaattct tggataaaat ccattaagct tttttggatc ttattcccag ctaataagta
      421 gagatatttg tccactgctt caactcccat atcaaaaaac gatatttctt gtgaatgagt
      481 ggctgctgcc cttttttgat tgttgttctt cagtcgtaag atgacaaagc atcttatctt
      541 tgaataaaaa aatagaacta cctaaggaag cgtttcggcg cttccttttt ctatatgatc
      601 gtttaatgac tgtaggaatt cagtatacca aaagaaaacc gcctttttga ataaacttga
      661 tagtggtcaa ttttttatga tggataagta tctatactta gtctcgtaag aatgaaaagg
      721 aaaagaggga gaaagatgaa attacaaaag tggatccaag agcatcgaaa tcatatcacc
      781 gccttatcag ctgtgttgat tatttttggt tttatcggga aatttactgg atttgtcttg
      841 ttctataaca ttgctctagc catagcttct attattgcgg tcatacctat tataatccat
      901 gcgtatcagg cagtaaaagt caaagttatc tcaattgatt tactcgttac tattgctgtg
      961 cttggtgcct ttatgatcgg agaatacaag gagtcagcta tcgttacatt cttgtttttg
     1021 tttggtagtt atctggaaca aaaaacattg accaaaacgc gggaatcaat caaggctttg
     1081 accgagatgg ccccatcgac tgccagcgtt gtccaagaag atggaacgac cgaagaagtg
     1141 gaaatcgatg acgtggaaga aggcgatatc gtcttagtaa aaactggtgg agctattcct
     1201 gtggacggac gagtcattga cgggaaaggg tatgcggatg aagccgcagt gacgggtgaa
     1261 tcacgacaag tcgtcaaaat cagtggagat aacgtatttt ctggaacgat tttagcagat
     1321 ggctacgtga cgatcgaagc aactaaggtc ggagatgata cgacctttgc caaaattata
     1381 gaattagtcg aagaagcaca agataccaaa tcacatgcgg aaaaattcat tgatcaattt
     1441 tctcagtatt atactccaac cgttttactt attgcgttgc ttgttggtct gatcagtcgt
     1501 gattttcgtt tagccatcac tgttttggtt cttggttgtc ctggcgcttt agtcattggt
     1561 gcgcctgttt cgaatgtagc aggaatcggt aatggagcga aacggggcat cttagtcaaa
     1621 ggcggtgaag caatggatac tttttccaaa atcgatactt ttgtctttga taaaaccggt
     1681 acattgacca aaggaactac tgctgtatcc actatcaaga actatacaga ggatgaaggg
     1741 gacagcttag ctttagtggc tcgattagaa acgttatccg atcatcctct ggggcgcgcc
     1801 gtgatccatt atgctcagga gaaagacatt gattttcaac aattagcagt caaagataat
     1861 cagacgatca agggacaagg aatcacagct gagattgatg gtcatacagt ttgtgcgggc
     1921 aacaccaaat tgattgccgc tcacaaactt tctctcacac caaagcaagt acaagattta
     1981 catcaattac aacaaagagg cagctcagtc attattgtag caatcgacaa aaaaatcact
     2041 caaatcattg gtgtgtcaga tgtgattcga ccagaagtcg ctgaacaatt agctatgttg
     2101 aaacaagcag gagcaaaaca tttagtgatg ctgacaggtg ataatcagat gactgctgat
     2161 tatgtagctg acatgttagg cattgatgaa gtccatgctg aattattgcc agatgaaaag
     2221 gttcaatttg tgaaaaaata ccaagaacaa ggattgcacg ttgctttcat tggagatgga
     2281 atcaatgatt ctccctcttt ggctgcggct gatatcggga ttgctatggg atcaggaacg
     2341 gatgtcgcta tcgaaacgag tgacgttgtc ttgatgcaat ctagttttgg atcgttggtt
     2401 catgcttacc gtttggctaa gaaaactgtt ttgaataccc gtgaaaatgt agtgattgcc
     2461 attggtgtcg tcttattttt actcatcggc ttatttgccg ggtttattta catggctagt
     2521 gggatgttcg ttcacgaagc tagtatcttg atcgttatct ttaacgcgat gcgtttattg
     2581 tattttggac acgaaagcaa agaagaaaaa tcgtaaaact tgataagaat caattttttt
     2641 atggaagctg taatctagaa taagactatc aagaaaaagg aaggaagaac tacgatgaaa
     2701 aaaatgatta tgcaattaga tacactagct tgtccaagct gtatgcaaaa aatagaacat
     2761 gcggtttctc aacagcctgg ggtaagcaat acgaaagttt tgtttaatgc gagcaaaatc
     2821 aaagcaaact ttgatccaga tgaagtccac cctgaacaat tagctgcagt tgtggaaaaa
     2881 ttaggttatg aagtccaatc aacaaaagta aaagaggcgt aaatcagtga atatagtgga
     2941 agaaaaattt gctcaagaaa caaaacaagc agatatcgat catcataaac caactgctgg
     3001 cgccatggtg gggcatgtat tggcaaattt taaattagaa gaaatgaaat tgatccaagc
     3061 acaattgtat gttttgggta catcttcgca agaattaagt accctttttt cagaatatgc
     3121 tgatcaagaa gcgagatggt tcaataaaat ctctcatgag ttagtgattg aaaatgaaat
     3181 cgttccgtct actttagaag aagtcatgcg ttatggaaaa atttacgaac atggtcaaaa
     3241 caaatattta ccagcctcaa caatgttgga aaactttgca aaagattttg atttccaaaa
     3301 tgtctttatc acacgggcaa tcaaattagc tgaaaaagaa gaaaaatatg gcttgaaaca
     3361 attgcttgtt gaacttttaa gtttcaataa agaaatcatc taccaagtac aaagactcca
     3421 aggaaaaact gtccgacaag atttggatga ggaagacgat gatgactaaa agtctatgtt
     3481 tcttcaaata caaatataaa gcgtgaggta aattggaaaa tcgcgaagat taaaataaaa
     3541 gaagcacccg ctgatgtatt acagacaaca tcagcgggtg cttcttagtt tttaaaaggt
     3601 ttaaaagatg gatgagaaaa tacaaaaaat caataaaatc acattgctta tttgatagaa
     3661 taaagaagtg ggagtaatcg aaaggagttt tttaatttaa aaaaaggatt ttagtaacaa
     3721 ttggtctagc ttttttgtta gctggttgta ccaacgggaa tgaatcgaaa aaagaatcaa
     3781 caaaagaaac agcgaaagca acaattgcaa ctactacaac aactagcaca gagagcaagc
     3841 caactgcagc aagtacggaa acagcagata cctctacttc gtcagagtct tccaaaacga
     3901 ctgaaacaac tcaaacatct acttcaagtt cttctgtaga ggcttcatct tcagaaagca
     3961 gcgaacaagt cgaacaaaca ctgtggaatg cagacaaatc cgcacaatta gaggcattta
     4021 tggctacatg gggccaaaca atgggacaac aatacaaaag ctatacgaat caaatgagtg
     4081 tggacttata tggtcttaaa gtccctcaag ttattttgaa cggggagtgg aagatggcta
     4141 ttggtggagt gcctgtttct gcagaatggt cagaaagtgg gacaggtcaa gcagactatc
     4201 aaatcacagc cgtctattcc gatgcagaga cagaaccata tctgaaaaag cacgtctatc
     4261 tattcgggtt tcagcaaaat cagccaaaag tattggtgac acaacaaaat caggggaatc
     4321 ccgataatta tctttacttt aacgagacag cgaataatga attgaaaaat ggctttaatc
     4381 aaatcgttta tgggtaagta aaccagtgag agtgagagaa agaagagact cagtggaaaa
     4441 tattaattga ttgctatgtc taagaattaa gatgaaggat gaaatttcta gtttcgtcct
     4501 tcatcttagt tacgttttac ttggataact ataataataa cgataaatag ttttctctcg
     4561 acttctttag agattgaata aaccctccaa tactaaaatt gaatgttgca agagcatgaa
     4621 ttgccaattg tattattaga atttttagaa aattgcacct ttagtttctt tagataaaat
     4681 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     4741 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     4801 ccccacgtgg acatttattt caatattggg gacgttgttt gtcagttgct tgaaggcaat
     4861 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     4921 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     4981 tgtagcagtc attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     5041 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     5101 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gttttattct gggctgtatt
     5161 gttgggacta gcttttcgag cacgttcaga accgacaaaa gagttggtgg atcagatatc
     5221 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     5281 attggtttat caatcaattg ctacgactgg aatcgctgga ttggcagagt atctgcagct
     5341 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     5401 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     5461 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     5521 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     5581 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     5641 agggattgaa gcaccatttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     5701 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     5761 gtttggtatc tctaatgatg tcgcgatgca aatcgttggt attggattta ttatcggtgt
     5821 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     5881 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     5941 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     6001 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgttt
     6061 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     6121 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     6181 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     6241 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     6301 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     6361 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     6421 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     6481 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     6541 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     6601 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     6661 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     6721 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     6781 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     6841 ctgtcttaga gaagcaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     6901 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     6961 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     7021 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     7081 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     7141 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     7201 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     7261 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     7321 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     7381 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     7441 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     7501 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     7561 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     7621 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgct cataaaaaag
     7681 aatcatcttt actcgacttc tgagaattgg agaacatccc ttttttcaga ggtcgttttt
     7741 tagttaaacc aaaccataga gtatgctttt taagataaag atcatcttat atcatctgaa
     7801 gctgcttatc ttctttgaat aaatacgatt caatcgatta tttcctcact ttatgataaa
     7861 ttacagctaa agtcactagc ttgtcttctt cggaaaggag gagaatctat ggatgaggaa
     7921 caaatattca ttcaaacact ttataatctg attttgaatc cgaatacacg agattgggaa
     7981 cgaaaagtat tgattcagac aaagaatgac acaagagaaa atatatccgt taaagaacag
     8041 ttatcaaaac tagaagtaac actacgtcca ttagctatcc gaatgaatct gacgcctgat
     8101 gtaatggact tttatttatt gattacggaa ggatttgata aggagcaaaa atatgacttt
     8161 tccaagcatg ccatgcaaga tgctgactat caagaacgtg cagtatttgc agggggatgt
     8221 ttctggtgta tggtcgagcc gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga
     8281 tacacgggcg gacatgttga aaaaccgaat tatgatcaag taagtggtgg ttacacaggt
     8341 catgtggaag ctgtggagat catttatgac acaagagaaa taagttatag cgagttgctg
     8401 acaatctatt ggcaaattac tgatcccaca gatacatttg gacaatttca agatagaggg
     8461 aagcaatatc gtccagtgat tttttatcaa gacgaacgac aaaaagaatt agcggaacaa
     8521 tcaaaacaga aactagacag ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa
     8581 ccagccggta cgttttggcc agcagaaaac tatcatcagc aattttacaa aaaacaaccg
     8641 aaacgctata aaaaaatcca gcaagcacgt aatcaatttc tgatctatca acgagtaaaa
     8701 aacaaatggc aaaaaaatat acgaaaaaat cattttgatt aactcctttt ctttaaaatg
     8761 tagtaaacta aaaatagaaa acaagaaagc gttttaaaaa gatttgatga ggaggagaaa
     8821 aattgtatat tgcagatagt gcacgttatg aaaaaatgga atatcgtaaa tccggtcatt
     8881 caggtttaaa attaccgatt ttatctctcc tctcggatta tggcaaaatt ttggtgatta
     8941 tgatcctatc cataatcaaa gagagatttt aagaggcgca ttcgacatgg gaattaccca
     9001 tttcgacctt gcgaataatt atggaggccc tgctggagct gcagagaaaa actttgggcg
     9061 tatttttaga gaagattttc aagcctaccg tgatgaactg attatttcta gcaaagcagg
     9121 ttatcatatg tgggaagggc catatgggga atggggctct cgtaaatcca tcatatcaag
     9181 ttgtgaccaa agtttccaaa gaatggggct agattatgtt gacatcttct atcatcatcg
     9241 cccagaccct gatacacctt tggaagaaac agcagaagcg ttgatgcaac ttgtaagaca
     9301 aggaaaagca ttatatattg gtatatccaa ctataatgga gaagatacaa aaaaatgact
     9361 gaaattttaa aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt
     9421 tctcgtgcac tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca
     9481 atcacgttca gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct
     9541 gaagattcta gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca
     9601 actctggaaa aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg
     9661 caaatagccc ttgcatggaa tttacgacaa aaatctgtta caagtgtact gatcggagca
     9721 agtcggttaa gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca
     9781 gaagaagaat tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat
     9841 ccatatatta aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg
     9901 aacatcatat tttttgtcga tcaaaataaa aatgatcgaa ggctaggagt gaattttatc
     9961 ctagtctttt ttatggtaaa tatcttcttc gtttgtatgt ctatgaacgg attaatcgat
    10021 tttttataca aaaaatcatc agatgattca aacgtgttat aataattacg aaaaaataca
    10081 tcagatgaaa aggaggtatg tggtttggaa gaaaataaaa ccctagtaga agtaactact
    10141 gacaagatcc ttagatattt aaaggaaatg cagttgcaag taggagataa actgccgaat
    10201 gaatatgaat tgtccgatta tttagaggtg gggcgaagca cggtgcgaga agctgtgcgg
    10261 gctctagctt ccaggaatat ccttgaagtt cggcaaggag cggggacatt tgtcagtgat
    10321 aagcgaggga ttgccaatga cccactcggt ttttctttga ttgaagatca ggatcggatg
    10381 atacgagact tatttgaact gagatattta ttggagccta agatggcagg tttggcagcg
    10441 gaatatgcaa cgaagaatca aatcgtccaa atgaaaaaat taacgcaaaa gattgaaact
    10501 tcttttcgag aaggcacaca agaacatgtt caattagaca tcgattttca tacgatgatt
    10561 gcagaagcta gcggaaacat agccttgctg catattatcc caatcattaa tgaatcgatt
    10621 gctttgttta ataaaaatta tgatatccag catctgaaaa tcgagacgat cgatattcat
    10681 acagagatcg ttcaggccat cgaaaggcgg aacgtacttg ctgcgtttga cgcaatgact
    10741 gtccatatgg caataaatcg gagagaatta cagaaaataa taggagaaaa agaaactaat
    10801 tgacagttca tctgatgtat tttatgataa atacatcaga tgattttttg ttttatattt
    10861 ggtaaaaaag aggagtaaat agtttctgtt caccaaaatg taagcgctga ctaatagaca
    10921 aaaagagatg acgtcaagga gaagaagcag gaatgagggg atctggaatg agttttatgg
    10981 ataaattagc caaagtttta gggaacgtgg caacgagaat caataattta aggtatatca
    11041 tggtgataaa aaacgcattt gctgcattga ttccggtaat tattacgggt gcttttggca
    11101 cattgttttc agcaatggtc tttgactcgg aaaatggctt agcacagata agttacttgc
    11161 gttttttaga agaattaaag cctatcgcat ctgccatcag ctatgtcaca ctaagtttct
    11221 tgaccatcta tgctgttttt ctaataggga tcgaattagc caagctgaat aaagtggatg
    11281 gtgtattccc tggtatcatc gcagtgatgt cttatttatc tgttaatcca actgtttatg
    11341 aattagtgaa tgaaaatcag actgttatag tagaaaacgt attggctaaa caatatacag
    11401 acacgaaagt ggtgttagta tcaaatctta gacagctttt cgtagagaca agaataaatt
    11461 aaacttatct acgagaggat gaatttttat gacccgatat gaagaaaatt ttaaacaaat
    11521 gatt
//
LOCUS       DACEDD010000090        11357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEDD010000090 DACEDD010000000
VERSION     DACEDD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.090"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(54..2756)
                     /locus_tag="IWO45_002716"
     CDS             complement(54..2756)
                     /locus_tag="IWO45_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0334157.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2769..4058)
                     /locus_tag="IWO45_002717"
     CDS             complement(2769..4058)
                     /locus_tag="IWO45_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0334158.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4208..5254
                     /locus_tag="IWO45_002718"
     CDS             4208..5254
                     /locus_tag="IWO45_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0334159.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5256..7409
                     /locus_tag="IWO45_002719"
     CDS             5256..7409
                     /locus_tag="IWO45_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0334160.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(7882..9333)
                     /locus_tag="IWO45_002720"
     CDS             complement(7882..9333)
                     /locus_tag="IWO45_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0334161.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(9330..11054)
                     /locus_tag="IWO45_002721"
     CDS             complement(9330..11054)
                     /locus_tag="IWO45_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0334162.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(11047..>11357)
                     /locus_tag="IWO45_002722"
     CDS             complement(11047..>11357)
                     /locus_tag="IWO45_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0334163.1"
                     /translation="LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVF
                     SSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE
                     "
BASE COUNT         3184 a         2290 c         1997 g         3886 t
ORIGIN      
        1 taaaatttct ccaccatctg aaaccgcaca cacaaatttt gttccgcctg cttctattcc
       61 gccatacatt cgttttcctc ctcgaatccg attgtccgaa tctcataagg ttggattatt
      121 gggacaaccg cttgcttcgt tggttcttca agcagattca atatgacagt ttttccattg
      181 tctttagtta tttccaattt ttctaaatga ctagacatat tgaaacctct tactactact
      241 ttatcactaa acttgctgcg tttcaaagct gtaattgcaa acgtttcact ttttatcgtt
      301 aaatattgtt gttttgaaat caatgttcct tcatgatgtt tgatttgttg agtggaaaaa
      361 ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt tttcttctgg tccatgtagt
      421 tcaatgctgt aattgaaccg atgctcacct agacactgtg cttctggtgt tggaaaatat
      481 ccccaatcgc ctagttctcc cacactgcga agtagcgtca ctgcgatttg tccgtcttct
      541 gttacttcat attcattcaa accaaagttt ccgactgtta caccgtactc ttcatcatga
      601 atattgacaa atgcttgctg atgttgcgga ttcgtggggt tttcccaact tttagacaca
      661 tggtttgggc gcttcaccac ttcaaaaata ctatctgctt cgtgatgttc cacatgtaac
      721 tttgtaggaa acaagacacg taagcgatga tctttcatct gattgtcgag agtcgtttca
      781 aagtcgattt ttttactatc ttttctgatt gtcatgattg tttttatttg aaggattcgt
      841 ttttctttgg atcgttctgc ttttcgataa cgaaattcca caaccatctg ctgttctttt
      901 tctaaacgct cgtctgcaga aacagggatt tccatttcct gtagaatact tatttttgct
      961 atttccggtg tatctactaa tacttttact tcagagtttt ctttgttact ggataggatt
     1021 ggctgatcac agaaaggttg tttaaagata tactcatttc ctatatcgcc gatatcttca
     1081 aaaatatgca gattattcaa ctttcgtttg tttttgcgat cttccattgt aatggtacca
     1141 tttttttcaa tcgtcaaatg tagagggccg ttttcgatga ttcgtccact ctgatgaatc
     1201 attgtttcct tttcttctgc tttcgcttcc ccttctacaa gcgcgaatgt ttcccaagaa
     1261 aatgctggca tgttttctaa tggtaaagtc actttgatca ttcttgccat atatggcaca
     1321 cggaaacggt ctttcggcaa atcataacca aattggacag tttcttcaga taaaatagct
     1381 ggtatagtgg ccccactttt tgtttctaca tgatacttac gtttaggcaa attttctaac
     1441 tcttgatata gttgttcagg tattccttca gcgaatcttt ttctttctaa ttcgatggtt
     1501 atttctgctt caccagtttt atccattcct gcagtattta caatgacaaa tggaaagctg
     1561 tcttttggga atccggtcgt atcgattgct gcggtcagct gttctaagga atcttgtgca
     1621 agatactttc caacttcatc tgctttttca aaacgaggta tcatttcccg atggacagaa
     1681 tcgactgaac aaccgcaaat actgtcgtga ggatgatttt gcattaatgt cttccaagca
     1741 taatctaatt gatcatgggg atagtttcca ctaacttcat aagccatcgt cgcaagtggt
     1801 tctgtaatat tttctagctg tcgctggacc ttggtattcc attgttttaa gtaaacccga
     1861 gcagatgctg tattggctaa agtataccag ccatccgttt cttgacttgt caattctcct
     1921 tctactgagc ctaagtcttc tggcacatct ttttgaactg cttccagata gtcagtaaaa
     1981 ttagaatgaa taaactcgta atctggaaac agttcatttg cgagatggat tgcttttgat
     2041 atatcttttt gtaccggttg atgatcaaca ccattcatca tcaataagtg gttcgtagag
     2101 gcatattgtt ctgcatccgc taatttttgc ttccaaaagg caagagctgc ttctttttct
     2161 actggaatct cattcccgtt gctataccaa ttggcaaata gcagaccaaa gattgctgtt
     2221 tgatcaggtc ctttccacca catctctgag tattgggaag aatagttttc tgcttccaaa
     2281 acttgattat caaagccaat tggttttaca ccacggccaa acgctgctgc tgaaatacca
     2341 gcttgtttca ttaattgagg cgtctgtccc atatttccaa atgtatcggg aaaatagcct
     2401 aacatgacag gggttcccca tttacggctt tcttccatgc caatcagcat atttctcacg
     2461 ttcgattccg agctgataag aaaatcatct tgcaagatat aaaatggtcc aattctcaat
     2521 tttccttcat tgattgcttg ttggattgcc tgtctttttt ctggccttac ttgaagatag
     2581 tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt tgaaggacgg atcaacttca
     2641 aatagctcta gtagatcgtc tatcaattca atcagtcgca tgtgatgctg ttcgtaagcc
     2701 atgtaccatt cacgatccca gtggctatga gaaatgatat atactttctt tttcatttac
     2761 gatacctcct acttttctac tctaatatca aagtaatcca tgactaattc gcaaaacatc
     2821 atatttgccc aagaaaacca ttctctcgta taattttcag gattgttcac atcgaatcct
     2881 tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta aattcaatat ccgctctttt
     2941 tcagatttgt ctgacgttgt cattccttcc atagctaatg caatcggcca aacgtatttt
     3001 tctggcgtat gggaggatcc aattcctttt gcgtactttc cttcatagaa atatggattt
     3061 tctttactca acaatgttct tcttgtttgc agataatctt cctcttgttc agaacaatat
     3121 cccaagtaag gtgcggcgat cagattgggc acattactgt catccatgac tgtggcgttt
     3181 cccattccat ctacttcata tgcataaatc gtttgccctt tttgattaac cgttcgtccg
     3241 tgattctgaa tcccctcttg gatctctttc ttcaatttct gtgtttgctg tacaatttct
     3301 ttatcatcga gtatcgtttg aaaaatttct tccacataac ctaacacgac caccgcaaac
     3361 atattggatg gtaccagata tccataacgg caagtatcat cgctaggacg gaaaccagac
     3421 catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt cattgatcag cgtatcttct
     3481 tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt catgatcttg ctctgttttg
     3541 aacacgtcca agattttttt gataccttca ataaaagatt gattgaattg atcagttttt
     3601 cctgtatttt tgtaaagaag ataggccaac tgtattgggt agcataaaga atcaatttca
     3661 tattttcttt cccagatcca atcgttcatt tttgtatgat ctgtttggtg gccagcaccg
     3721 ttagcttctt cgttgaaggc attcgcatat ggatcgatat tgatataaaa aaattgcttt
     3781 ttgactaacc cgctgatcat gtcagctaat tcctcatctt cttttgcaat cactaaataa
     3841 ggacgaactt gtgcagtaga gtctcttaac cacatcgctg gaatgtctcc cgtcaataaa
     3901 aaggtggttc cgtcattttg ctttttgact gttgtcaaca gagtattggc aaatgctgct
     3961 gtaaaattct ttgcccagtc cgcatggata tccccacatt tttgtgtgat atcttccata
     4021 aatttctgca ccgattttgg tatatcttta taagccacta ataaaacctt ctttctattt
     4081 gatatatctt taaagattat acttcataca gccaattatg tctacgcttt caaaaaatat
     4141 ctttttattt actttttcat ccattatggt attatttgaa tatgatatat cattaagagg
     4201 tgtttatatg aatcaaccat tgtaccagac aatatttaat gatttgcaaa cggctatttt
     4261 aactggtgaa ttacctgtag acagccaact accaactgaa aaagaacttt cacgtaccta
     4321 taaagtaagc agaatcactt ccaaacgtgc attgactgaa ttagagcaat taggacttat
     4381 ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct tctccggaac agattcttgc
     4441 ttctcctaca aaaacacgac ggattctgtt tcttcttcca tatgtcgctg atttgtctgt
     4501 tggtgatttt agcaagggat tgaaccctgt gatgcaagat aaacagattg atgtcatgat
     4561 gacgacgctt gactttctac aaaataaaac agcaaaagag ctaatcaatg aatttgacgg
     4621 attgatttat tatgctcttg atacagaaca acatcttgac ttactttttg agctttcatt
     4681 gaaagaattc cctgtcgtca tattagataa aaaaatctat gagttacctt ttcctaccgt
     4741 tctttccgat aatttccaag gcggtgcgct tgctgcctct tatttgatcg aacagggaca
     4801 ttcaaaaatc gcttatttgt ttggtacgca gactcatcca caatccgttc gacagcgtta
     4861 ccttggttac cttgaagcat tggacaaagc tggtttgact ttccacactt cactggatga
     4921 caaaagcgcg acattagatg aattgctttc ttatatcact aagtatgaag taactgcttt
     4981 tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat ctcttgagac agcatggtta
     5041 tcatatccct gaagattttt caattatcgg atttgatgat atccaagcag cagctcttgt
     5101 tgacccgcca ctgacaacca tcgcacaaga ttttgaacag ttaggtaagc ttgcgggaga
     5161 tgctttggtc acttggttag agacaaaaga tcgaccatcc gatagtaaat ttccagtttc
     5221 attcatcaaa agacaatcaa caaaggagaa ataaaatgaa tatcggtcaa atagatacta
     5281 gacatgcaac agcgaatcag ccaaatttct ctaatgggaa ctgccagcct tatactggcg
     5341 ttccttttgg gatgaattat tttgcaccgc aaactacgga tcaaaacggc agctggtggt
     5401 ttcatcctga tgaccgcgtt tttcaaggct accgtcttac ccatcaacca agtccttgga
     5461 tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg aaaactacag gagaacaccc
     5521 ttttccatgc tcaaagttct taccgcccag aagaaagtat attttgcccg actcatctgt
     5581 cgatctgtca attacgttat ggcatctgtt ctacattgat acctagcatg tatggcggaa
     5641 tcttatctat tgattacagc aaaaatgaca gcggtttact tttatccttt ccgggacgcc
     5701 atcagctctt tgttatagat ccttacacag tggaaggaaa aatcacacat tttgctggtt
     5761 cagaagatcc tgatttttct ttttatttta tattgagatt tgaatatcct ttaacaattg
     5821 atgagttagc tgtttccaac aaaaatagcg actcgatccc gttgtatttt gaacagacaa
     5881 aaaaacaaac tatccgattt gggacatctt ttatcagctc ggaacaagca gcttataacc
     5941 tggaactaga acaaaaccaa agcaaagaga cttaccttgc tcacagtcaa gaacaatggc
     6001 agtcctattt tgatcgtata cagctcacac accatgatca acaacaattg cggacgtttt
     6061 atcataattt atatcgggtg tttttatttc cacaaacatt ttatgaaatt gatcctagtg
     6121 ggcaaaaaat ccactatgat acgactagtc gatctataaa acctggtgtt ctctatacaa
     6181 ataatgggtt ttgggatacg tataaaacgg tttatcctct ttattctttg attgctgtag
     6241 aaaaatacga agaaatgctg gaaggttttt tgaacagcta tagagaaagt ggctatttgc
     6301 caaaatggct gtctcctgat gaacgtggac ttatgcctgg gacattgatc gatgcggtca
     6361 ttgcagatgc tgctgtaaaa gatatccgac gagacttgat gcctgaattt ctaacggcaa
     6421 tggaaaaagg tgctacggtt caaagcaaac atccaaatta tggacgccaa ggaacagaag
     6481 attacttgac atatggctat gttcctaatc actaccacga atcagtcaat cacacattag
     6541 actatgctta tagcgatttt tgtattagcc aagttgccaa ggtactagaa gaagaggaaa
     6601 tgacgaagaa atatcagcag caatcattga actatcaaaa tatctttgac tatgagtcag
     6661 gatttatgcg ttcaaaagat aaagatggca agttcagaga accattttca gctaatcgtt
     6721 ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag ctttgctgtt tatcacgatt
     6781 tttcaggact gatcggaaaa tatggtggca gtgagagata taaagaaaaa ctcattgagc
     6841 tgtgcaatca gcccccaacg tttgacccag gaggatatgg ttttgagatc cacgaaatga
     6901 gtgagatggc cgcgatagaa tttggacaat tagctatttc aaaccagcca agttttcact
     6961 atccttactt gttcagttat ataggacaac ctgaaatggc acagccactg cttaaacagt
     7021 tgatgactca gaccttcgac gattctccaa tgggttaccc tggagatgag gacaatggaa
     7081 gtatggccgc ttggtatatc ttcaatagtc tgggatttta tcctgtcacg cctggaaccg
     7141 gtgaatatgt tatcggtatg ccattaattg acaaggctgt tttgcttttg tcaaatggaa
     7201 aggaattgac gattgaagca acgcctaatc ggccgcagca acaatttatc cataaaatca
     7261 aacggatgga caaagaatat acgaaattat attttaccca tcaagatttg ctgaaaggcg
     7321 gcacgatctc ttaccaatta gggatcatcc ctcaccctaa gaaatatcaa gcaaaagatt
     7381 tgcctttttc cttatcaaac tataattaac aagaggttgt gaaaaagctg tcctgcatca
     7441 agtaataaga acagccaagc aaaaatagat cctcatattt ttcgtttggt tggtcttatt
     7501 accgcagatg caagcttttg aacaccgttt attgagaggt tgtgaaatca gcgttctgac
     7561 ctgagtatta gagagacaaa tggcaaaata gttcctcata ttttgccatt ttttgagcta
     7621 ataccgaggg tcagctgatt gaacaccgtt tagtggagag gtcgtgaaaa agctgtttag
     7681 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc
     7741 ttaataccga gaaactggct tttgaactcc gtttatcgag aggtcatgaa aaagctgcct
     7801 tgcctcaagt aataagaaca gcccaaaatt agatttctag acaatttttt ataagttatt
     7861 tcattacatt ggctttttct tttatgattc tccactgttt tctcggtact ctttagggga
     7921 tctgccgacg attttcttga ataatttaga gaaatacgtg gtgttgttgt agccaatctc
     7981 acttgctatc tcattgatgt tttgattact gtgcaaaagc agttgtttcg ctttttccat
     8041 acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt tcttttttga acagctgtcc
     8101 tagatacatg acatttaaat gcaggttttc tgacacttct ttgagtgtaa gtggttcttg
     8161 ataatgctgg tgcaggatta cgattacttt ttccacattg tcactgtact ttttctgtct
     8221 ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt tcttgcaccg tttttgcctg
     8281 atggattttt tcgattcctt gtaaatattc ctcatcttcc agatcaatca attcccgttg
     8341 aatctccata aacagtaaaa aactggaata tcgtatatct tccggcatca tggctgctaa
     8401 ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg acttccaatt gtttattttg
     8461 cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct tttgtttctg cttgtactgc
     8521 gtaaaaaaca tgctcgtctt tatctccata aaattgatgc aactgtagat tgtctttcgc
     8581 ttgttggaat gtttcaggga ttttgtccaa agaatctgtc tcttccccga tactgatcag
     8641 ccattcctga tcaggaaagt tttcttgaac aaaacaacag aagccatcaa cttcatctgc
     8701 ctccaacaac aacgtgatca atacacggtc tccgtaattt cgttgatagt aaaagatttg
     8761 ttgccgttct tgcaaccatc tattgataag agtatcatga gttcttgcca gttgaatcaa
     8821 tactatccta tgctgcttct cacttatttg tcctattatt tcttcttcac ttatttcatc
     8881 caattcttca ttcaaccatt gactgaataa cagttcttga tagatttctt gttgattttt
     8941 ttgttcattt tgctgatcta accgtgtttt gattttcttg aggctttcaa ccaattcgaa
     9001 cttattcacg ggtttcatca aataattcac ggcgcctaat tgcatacctc ctttcaaata
     9061 atcgaattct tggtatcctg ataaaatcat aaattcaaaa ttatgacgtt ctttttgagc
     9121 cgcttcaata aattccagtc catttatttc tggcattgtc acatccgtta ctactaaatc
     9181 aatctgtcgc tgttcaagta cagctagtcc ttctttggca ttctctgctg ttgctactat
     9241 ttgaaagcct aaactttgcc aatcaattat cttttttaac ccattcaaaa tcatgtattc
     9301 atcatctacc aataacactt gatacacgct catccctcct tgaatgtaat agaaacggtc
     9361 agtccacctg tctcattatt ctcaagcgac atataataac tgctgccgaa gctggcacgt
     9421 aaacgttcat tcacgttttg cagaccgata gaaccgtgaa gttccacttt aggatgagac
     9481 agtttttctt caatctgttt tagccgtttt tcagtcatcc cttttccatt atctttgatg
     9541 atgatcctta cccgtttgcc ttcctgcaag actttgacac ttagcgcatt atcgaatcga
     9601 gtaaaatcaa taccgtgttt aaaataattt tcaactaatg gctggatcac aaatttaggt
     9661 acttcgatct tttccaaatc aggatcgatc ataaaatgat aggctactcg attaggataa
     9721 cgcatttgat acaaatagac atatttttcg caaaagtcca gttcttcttt taaagtgatc
     9781 gttttctctt gattcgtatt gtttcgaagc aaagcggaaa acgcatacac aacatctgct
     9841 aattcttcac ttccttcgct gattgcatac attcggatat attctaatgt attatataaa
     9901 aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg cgtcttgctg tttgatttct
     9961 aatttgtaga tgtcttctac aaactgattg atgctatcca acatttcgtt gataccgatt
    10021 gaaagttcct tcagttcata ttccgtgttt gttatgtcga tccgcgtctc taaattccct
    10081 gctgctgttt gatccatggc ctgcatgacg atagatactt gctgtgaata tcgtttgaat
    10141 gttttataaa gaaagataag cagtatattg tctaaaataa tgataccaat gatcagcggg
    10201 gcaagatttt ttcccgtttc gatttttaca ctttttttat ctagtacagc taatatacga
    10261 taatcatcag acagttcttg gtgttgaaca aaattgtttt tttccagatc gtcaatcggc
    10321 aattgtgaat ttttcttcag actctctgtg atgatcattt cttgtctttc aaacgtttct
    10381 ttagctattt tattatcatg gaatgtgtat agtcgatttg tcgttccaga aatcatgtat
    10441 aaagagagtc cattgaatga cgttagattt tctaaaatca tatctagttc tttcttttga
    10501 aaaccaataa aaatacttcc tgactcagct cctccttgtg tgataggcaa cttgatataa
    10561 aaggcgtcta gtaattttgg tgtccccttt tttatttccc cacctttgtt ttctctagaa
    10621 gattcataat actccggtag ttgattcaac acgatatata tcgcacttag ttcttcataa
    10681 tttgcgtaga gatctttgat ttgatttggg aaagaaacta attcatcggt actgttttgc
    10741 tgttcgtata catacatcag atactgatct atgggttttg ttaagtaggt cgtcacattt
    10801 tcgatctttt cggaattccc tacaagctgg tttttgatga tcctactcaa ggttgtttta
    10861 tcattcagca ttctgcttgt gctttgaacg gcatcacttg tcatgttttc tgcctgtttt
    10921 tgattttgtt cccaagtatg gatactaaaa ataacggtag cagtcatcgt tacaccgatc
    10981 atgataaccg cgtatttccg caacagacga tttagtaagg ttccttttga aaatagaatc
    11041 cgaaacttat tcataacttt ccaactgttt atctaattct tcacgccaat gtgttgtcat
    11101 cgtaccattc caaaccgtca aaagaccaat gacaccaaac aggatcaatc ctttgaattg
    11161 ccaagtcacg cctaaaatta taccgctacc aataattatt tttagaaaag acgaaaagct
    11221 ggaaaaaacc gaaataaaac ttagctttag cagattagga acagagatcg tatattcact
    11281 atctaaaatc atactgtatt gataagtagc aaataggcag agcaaggcaa aaagtaaaat
    11341 aaaatcaata gctaaaa
//
LOCUS       DACEDD010000091        10761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEDD010000091 DACEDD010000000
VERSION     DACEDD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.091"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..1042)
                     /locus_tag="IWO45_002723"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="IWO45_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="IWO45_002724"
     CDS             complement(1437..2279)
                     /locus_tag="IWO45_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0334164.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="IWO45_002725"
     CDS             2604..3020
                     /locus_tag="IWO45_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0334165.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="IWO45_002726"
     CDS             3017..3487
                     /locus_tag="IWO45_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0334166.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="IWO45_002727"
     CDS             3501..4274
                     /locus_tag="IWO45_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0334167.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="IWO45_002728"
     CDS             4288..5100
                     /locus_tag="IWO45_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0334168.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="IWO45_002729"
     CDS             5154..6119
                     /locus_tag="IWO45_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0334169.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="IWO45_002730"
     CDS             6256..8979
                     /locus_tag="IWO45_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0334170.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="IWO45_002731"
     CDS             8992..9624
                     /locus_tag="IWO45_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0334171.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="IWO45_002732"
     CDS             9829..10056
                     /locus_tag="IWO45_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ0334172.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="IWO45_002733"
     CDS             10125..10391
                     /locus_tag="IWO45_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0334173.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="IWO45_002734"
     CDS             10381..10650
                     /locus_tag="IWO45_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0334174.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3567 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt a
//
LOCUS       DACEDD010000092        10661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEDD010000092 DACEDD010000000
VERSION     DACEDD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.092"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            333..2279
                     /locus_tag="IWO45_002735"
     CDS             333..2279
                     /locus_tag="IWO45_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0334175.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="IWO45_002736"
     CDS             2282..2737
                     /locus_tag="IWO45_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0334176.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="IWO45_002737"
     CDS             2751..4079
                     /locus_tag="IWO45_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0334177.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="IWO45_002738"
     CDS             4113..4397
                     /locus_tag="IWO45_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0334178.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="IWO45_002739"
     CDS             4399..4914
                     /locus_tag="IWO45_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0334179.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="IWO45_002740"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="IWO45_002740"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0334180.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="IWO45_002741"
     CDS             5599..6171
                     /locus_tag="IWO45_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0334181.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="IWO45_002742"
     CDS             complement(6358..7878)
                     /locus_tag="IWO45_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0334182.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8121..8306)
                     /locus_tag="IWO45_002743"
     CDS             complement(8121..8306)
                     /locus_tag="IWO45_002743"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0334183.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="IWO45_002744"
     CDS             complement(8290..8472)
                     /locus_tag="IWO45_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334184.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="IWO45_002745"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="IWO45_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="IWO45_002746"
     CDS             9426..10025
                     /locus_tag="IWO45_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0334185.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..>10661
                     /locus_tag="IWO45_002747"
     CDS             10089..>10661
                     /locus_tag="IWO45_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334186.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1674 c         1990 g         3472 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tgagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga t
//
LOCUS       DACEDD010000093        10493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEDD010000093 DACEDD010000000
VERSION     DACEDD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.093"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..357)
                     /locus_tag="IWO45_002748"
     CDS             complement(<1..357)
                     /locus_tag="IWO45_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0334187.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IWO45_002749"
     CDS             complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IWO45_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0334188.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IWO45_002750"
     CDS             complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IWO45_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0334189.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IWO45_002751"
     CDS             complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IWO45_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0334190.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2237..2869
                     /locus_tag="IWO45_002752"
     CDS             2237..2869
                     /locus_tag="IWO45_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0334191.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2871..3776)
                     /locus_tag="IWO45_002753"
     CDS             complement(2871..3776)
                     /locus_tag="IWO45_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0334192.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3861..4514)
                     /locus_tag="IWO45_002754"
     CDS             complement(3861..4514)
                     /locus_tag="IWO45_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0334193.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4836..5780
                     /gene="mvk"
                     /locus_tag="IWO45_002755"
     CDS             4836..5780
                     /gene="mvk"
                     /locus_tag="IWO45_002755"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0334194.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5773..6750
                     /gene="mvaD"
                     /locus_tag="IWO45_002756"
     CDS             5773..6750
                     /gene="mvaD"
                     /locus_tag="IWO45_002756"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0334195.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6763..7848
                     /locus_tag="IWO45_002757"
     CDS             6763..7848
                     /locus_tag="IWO45_002757"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0334196.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7845..8909
                     /locus_tag="IWO45_002758"
     CDS             7845..8909
                     /locus_tag="IWO45_002758"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0334197.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9158..9961)
                     /locus_tag="IWO45_002759"
     CDS             complement(9158..9961)
                     /locus_tag="IWO45_002759"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0334198.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="IWO45_002760"
                     /pseudo
     CDS             complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="IWO45_002760"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10268..>10493)
                     /locus_tag="IWO45_002761"
     CDS             complement(10268..>10493)
                     /locus_tag="IWO45_002761"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0334199.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3104 a         2220 c         2008 g         3161 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga
      661 aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa
      721 agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc
      781 agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga
      841 gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa
      901 gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc
      961 ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac
     1021 tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc
     1081 ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt
     1141 gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc
     1201 gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc
     1261 cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca
     1321 tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt
     1381 tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc
     1441 gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt
     1501 caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc
     1561 tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc
     1621 attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt
     1681 acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg
     1741 cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc
     1801 aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa
     1861 tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca
     1921 cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt
     1981 tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag
     2041 tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca accattatag
     2101 tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa
     2161 aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat actaaagaaa
     2221 aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac
     2281 aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggagg
     2341 taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact
     2401 tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg
     2461 tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg
     2521 taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg
     2581 tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg
     2641 tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa
     2701 tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa
     2761 tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaatcaaga
     2821 gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc
     2881 cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt
     2941 tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc
     3001 acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc
     3061 aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct
     3121 aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa ctcttctttt
     3181 tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa aagtactaca
     3241 ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc
     3301 agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc
     3361 acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa
     3421 ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg aatcacgtac
     3481 tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga
     3541 ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac
     3601 gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc
     3661 agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt
     3721 tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc
     3781 tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc
     3841 tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc tgctaatttt
     3901 tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg
     3961 tctccatcga tttggatttt tggactttct ttgtattctt cataccattg atcataacgt
     4021 ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg ttcatacgaa
     4081 cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat atgaactaat
     4141 aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt
     4201 tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa
     4261 agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt
     4321 ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca
     4381 gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga
     4441 ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct
     4501 gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac
     4561 tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt ttttctagtc
     4621 aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta
     4681 ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc
     4741 cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc
     4801 ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat
     4861 caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg
     4921 cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg
     4981 atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa
     5041 atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac
     5101 tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca
     5161 cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat
     5221 tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga atcgatgcag
     5281 ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt gactactttt
     5341 ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag
     5401 aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga
     5461 tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag aattcaccag
     5521 aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg acaatcagca
     5581 atgattttct taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat
     5641 taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc
     5701 aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag
     5761 gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa
     5821 tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca
     5881 ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa
     5941 ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag tcagtttctt
     6001 gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc
     6061 gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggagct
     6121 tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt tgctcgacgc
     6181 gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga aaaaggccat
     6241 gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca
     6301 atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt
     6361 cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta
     6421 tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa
     6481 agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg
     6541 tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa aggcattcca
     6601 tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta
     6661 tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcccat
     6721 gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca
     6781 ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca tccagcagtt
     6841 atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa agtcggaagt
     6901 atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta
     6961 gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg tttgactgaa
     7021 aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag
     7081 ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca
     7141 acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga gattttcaag
     7201 attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct
     7261 agctgttatg gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa
     7321 gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct atccattgag
     7381 ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct
     7441 acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt ggcttatcag
     7501 cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat
     7561 aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct
     7621 gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa
     7681 cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg aatcgtaatt
     7741 gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc agaaatcact
     7801 ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg
     7861 aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac
     7921 ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg
     7981 cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa
     8041 aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg
     8101 ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact tatacgatta
     8161 tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg
     8221 aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg
     8281 cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta
     8341 ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca
     8401 tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg
     8461 gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga
     8521 gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag
     8581 acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag
     8641 ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg catctgatga
     8701 atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc
     8761 tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca ctgatctttt
     8821 ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac
     8881 gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct ctaaacacgt
     8941 ataaacggcg gaagcaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga
     9001 aaagcaattt tcgtgaattc cttcttattt cttggagtta aacgcttctg tcccagcctc
     9061 ttttttcgaa taaatgatgt ttaatcagct gtccttcggt attcgttcaa gccgctgatt
     9121 tcacaacctc tccactaaac ggtgtactaa agctctttta aggttgtttt ccgatatagg
     9181 caagtatgcc tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag
     9241 ccaagaagac agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac
     9301 gtccaacgat aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg
     9361 gagcagtttg cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt
     9421 gaccgtattc agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat
     9481 aagcactgac cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac
     9541 cgccgccttt ttcgatcata tcccgaataa ctgcttttga aacgatgaat ggggcattta
     9601 aatccacgtc gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt
     9661 tgatgatccc tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt
     9721 gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga
     9781 tgcctgcttc tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa
     9841 atacgatttt tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga
     9901 cagcaccggt gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca
     9961 tcattttctc tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta
    10021 taggtgatat tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct
    10081 gaatggatcg accagcttgg cgaaataatg gcttgttcat tgtctacgac taaatgtttt
    10141 gtttcatctg gtttgcccat catatggaac acacgtgtat cttcattggc ataatcaaaa
    10201 tagacataag cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcactt
    10261 cctggttcta aaatcgtata acccatttat aaatgacagc tatcgctgaa gcttctactg
    10321 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    10381 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    10441 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac aaa
//
LOCUS       DACEDD010000094         9484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEDD010000094 DACEDD010000000
VERSION     DACEDD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.094"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            125..1429
                     /locus_tag="IWO45_002762"
     CDS             125..1429
                     /locus_tag="IWO45_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0334200.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1521..1886)
                     /locus_tag="IWO45_002763"
     CDS             complement(1521..1886)
                     /locus_tag="IWO45_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0334201.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1883..2248)
                     /locus_tag="IWO45_002764"
     CDS             complement(1883..2248)
                     /locus_tag="IWO45_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0334202.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2440..2688)
                     /locus_tag="IWO45_002765"
     CDS             complement(2440..2688)
                     /locus_tag="IWO45_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334203.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2687..2815
                     /locus_tag="IWO45_002766"
     CDS             2687..2815
                     /locus_tag="IWO45_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0334204.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2872..4566
                     /locus_tag="IWO45_002767"
     CDS             2872..4566
                     /locus_tag="IWO45_002767"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0334205.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4648..4839
                     /locus_tag="IWO45_002768"
     CDS             4648..4839
                     /locus_tag="IWO45_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334206.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            5217..6350
                     /locus_tag="IWO45_002769"
     CDS             5217..6350
                     /locus_tag="IWO45_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0334207.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6381..6701
                     /locus_tag="IWO45_002770"
     CDS             6381..6701
                     /locus_tag="IWO45_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334208.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6730..7194)
                     /locus_tag="IWO45_002771"
     CDS             complement(6730..7194)
                     /locus_tag="IWO45_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0334209.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7323..8572)
                     /locus_tag="IWO45_002772"
                     /pseudo
     CDS             complement(7323..8572)
                     /locus_tag="IWO45_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8601..8918)
                     /locus_tag="IWO45_002773"
     CDS             complement(8601..8918)
                     /locus_tag="IWO45_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0334210.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8944..9264)
                     /locus_tag="IWO45_002774"
     CDS             complement(8944..9264)
                     /locus_tag="IWO45_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0334211.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3055 a         1870 c         1691 g         2868 t
ORIGIN      
        1 ccccttttaa ttagatttct agtctaactt ttggggtgca catcatttat gtgggcaatt
       61 ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg tttgcaaaac
      121 cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga tcgcttatac
      181 acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc gatgcaagat
      241 catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt atttggaaga
      301 agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca gcaaagctta
      361 ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa tcgaccgcta
      421 ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt
      481 aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg atcgtttcat
      541 tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag aaatcttatt
      601 gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa
      661 atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag catgggaaaa
      721 cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga cactattcaa
      781 tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc aacagctaca
      841 ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct ttgaatctca
      901 cttcaatcaa gaattattcg gaaaccaacc attcgaacgt gcgctgattc atctgatgcg
      961 ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttc taaatgagga
     1021 acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca gccagttacc
     1081 ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat tgagcggaat
     1141 tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg atgttcatta
     1201 tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag ttgcaccaac
     1261 aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa accgggtatt
     1321 atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta ttcctatttc
     1381 gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag ggactgtgac
     1441 aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac aatctcttga
     1501 tacacatcag gctcacaacc tcacttattt tggacatcta cgggtccgat ctctttattt
     1561 aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa aatatcctgc
     1621 aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc caaacttaga
     1681 agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg gtcgctttgc
     1741 acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca aacaccccaa
     1801 accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcaaa atatggaaga
     1861 agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt tctttgtctt
     1921 ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata agtgcaagct
     1981 tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca acgactagta
     2041 cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca ttacgagtat
     2101 tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat agaacaagaa
     2161 cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg atccattcta
     2221 gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta atttgttcct
     2281 attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt tctaatgata
     2341 acggttcgct taaatttttc tttttcactc tccccttttt ctccctccat ccataaatcg
     2401 tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca tcagcttttc
     2461 aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag aaccatacac
     2521 ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat cagacacttc
     2581 ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt ctaataaaaa
     2641 cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt
     2701 aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc taggaaaaaa
     2761 gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa tctagcatcc
     2821 tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac aatgagaaaa
     2881 agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt ttatttgatt
     2941 caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga tcacggttca
     3001 aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa ccatccatta
     3061 gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta
     3121 gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat gactgctgaa
     3181 gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg tttgatggaa
     3241 ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga ccgtattctt
     3301 ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa tatcactatc
     3361 gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt tatgtgggaa
     3421 acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa
     3481 atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac acgttacaat
     3541 caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg atgcaaaatc
     3601 attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca agatgaaatc
     3661 actgtgactg gtttgaaaat gaccaataca ctgactcaag aagaagaaca cgttgtggtc
     3721 gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc tactttagga
     3781 gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag tctctggaaa
     3841 aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc tgatcgagat
     3901 gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt gaatgaaatc
     3961 acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc aaccacaccg
     4021 atcacgccgt taggccaaaa agatgtcaat atgtctatcg tcgtgcatca tcaaccgcat
     4081 tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta tccaagacga
     4141 cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat gctagaagaa
     4201 ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga aaaagaaaca
     4261 gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc ttccgcgttg
     4321 ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc gaccaatctg
     4381 gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga acaaagtgct
     4441 gtacgttctg gagaaatcgc tgctttccat tttgcgggta tccctatgtc acgtcttgtc
     4501 aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa aaaaatgttt
     4561 gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac aaccaggcta
     4621 aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca agcttttgaa
     4681 caccgtttac caagaggttg tgaaaaagct gccctgcatc aagtaataag agcagccaag
     4741 ctaaaacagc ttcttatgtt tttgacttgg ttggacttat taccgcagat gcaagctttt
     4801 gaacaccgtt taccaagagg ttatgaaaaa gtcgtttagt ttcgagtatg aagaaaaatt
     4861 ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc
     4921 cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac cattttttca
     4981 acgtttactc tcttgccaat ccaacatcat ctaacagata caactatttc aatgattacc
     5041 attacactaa aaaatcggaa actcacctct ccgaacacaa atacataaga ctatagaatg
     5101 tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa
     5161 ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca tctccaatga
     5221 aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa
     5281 tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc
     5341 tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac
     5401 caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg
     5461 tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa
     5521 gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt
     5581 tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta
     5641 cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt ctatcagata
     5701 ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg
     5761 tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag
     5821 cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca
     5881 atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac
     5941 tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg
     6001 tcaaagaaaa agcgaaaaat ttagtaagct tacaaaacgg cacaatctct ccattacaga
     6061 tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc
     6121 cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac
     6181 aggcaccaat tatccatcta atgccaaaaa tttacgaaat gattgcagaa ggactattcg
     6241 atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaga
     6301 tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa
     6361 aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag
     6421 gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt
     6481 acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc
     6541 ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta
     6601 ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa
     6661 aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agatttgaaa aaatctcatg
     6721 cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc cactttcttc
     6781 cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat gatctgtaat
     6841 taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg ctaatatccg
     6901 cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa gatttggtgt
     6961 caaatcaggg cggttatgga agtcgttttc aataacgccc gctcctccga cgatggtttc
     7021 tttttcatct aagaccacat accattgagg aatactttcc ttattagcga ttccagtacg
     7081 aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc acttcgcact
     7141 ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa ccaatgttta
     7201 aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga aaattagcaa
     7261 tgacaacaat agtgatgtaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt
     7321 aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt
     7381 agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt
     7441 tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct
     7501 gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc
     7561 aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt
     7621 tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg
     7681 tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct
     7741 aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgtccagct
     7801 gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg
     7861 accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt
     7921 aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa
     7981 gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca
     8041 gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca
     8101 ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt
     8161 gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg
     8221 cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag
     8281 ccgttagcta aatgataagc aagaaagcaa tcaagacaga aagaatggca aatgtaccgt
     8341 tccaagcagc agccccgaat cctttccatg attcaccacc aaaaattgag ttcatcaatt
     8401 cttggaaccc gggaatcggt aagttattga tcattactgc taatgctcct aagatcatta
     8461 acggcatact caccataaat gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt
     8521 ttgatgctac aggtataaaa tgtttctcca taaatgcaac aaatttatcc ataaatcttc
     8581 cctcttttct ttactgtaga ttacgcgttc aatcgacgat acagatcaat gatctctttt
     8641 gctaaatctg taaaagctat ggatgtcatc agatgatctt gactgtggac tgtcaaaagc
     8701 gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt
     8761 gcttggacta aagattcctc tgcatctttg attttttcat ctgctaaagt gaaatcacct
     8821 tttttcgcag cagcaatcgc ttccattgca tcgctttttg catttccgcc aaacatgatc
     8881 agacccatga tggcttctag atttttttga tcttccattt attgtatcct ccgataattt
     8941 ttattatttg tttattagtt tttctgcttg gtcgagtact ttttcaccat tcatcatgcc
     9001 atagtcagcc atattgatta tatctaaagg aatgccttta ggtgctagct tttcagcaaa
     9061 ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa acgtctacat ttttagattc
     9121 tagttgatta tccgcatctg aagcagatac agcaaagatg tctgcttcca ttccttttgc
     9181 ttctgctgcc ttttgcattt ttgtgactag gagacttgta ctcattccag ctgaacatac
     9241 aagcatgatt gttttctttt ccattttaaa cacgttcctt tctatactta cacttgtata
     9301 taagcaagta gcgtgccaac tttgaaagcg ctttaaaaag ttgatatcat cacatttaag
     9361 gtctatacca agttgcatac acactaaaaa agaaagtgca cactaaacac acaaattaat
     9421 ttttattacc ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca
     9481 actt
//
LOCUS       DACEDD010000095         8942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEDD010000095 DACEDD010000000
VERSION     DACEDD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.095"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..260
                     /locus_tag="IWO45_002775"
     CDS             51..260
                     /locus_tag="IWO45_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334212.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IWO45_002776"
                     /pseudo
     CDS             467..667
                     /locus_tag="IWO45_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1154..1333
                     /locus_tag="IWO45_002777"
                     /pseudo
     CDS             1154..1333
                     /locus_tag="IWO45_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IWO45_002778"
     CDS             complement(1379..1984)
                     /locus_tag="IWO45_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0334213.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IWO45_002779"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IWO45_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IWO45_002780"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IWO45_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IWO45_002781"
     CDS             3019..3990
                     /locus_tag="IWO45_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0334214.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IWO45_002782"
     CDS             4130..5443
                     /locus_tag="IWO45_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0334215.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IWO45_002783"
     CDS             5486..6352
                     /locus_tag="IWO45_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0334216.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IWO45_002784"
     CDS             6349..7197
                     /locus_tag="IWO45_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0334217.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IWO45_002785"
     CDS             7211..8683
                     /locus_tag="IWO45_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0334218.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="IWO45_002786"
     CDS             8700..>8942
                     /locus_tag="IWO45_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0334219.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DACEDD010000096         8905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEDD010000096 DACEDD010000000
VERSION     DACEDD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.096"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            84..157
                     /locus_tag="IWO45_002787"
     tRNA            84..157
                     /locus_tag="IWO45_002787"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:118..120,aa:Met,seq:cat)
     gene            176..248
                     /locus_tag="IWO45_002788"
     tRNA            176..248
                     /locus_tag="IWO45_002788"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:209..211,aa:Asp,seq:gtc)
     gene            252..324
                     /locus_tag="IWO45_002789"
     tRNA            252..324
                     /locus_tag="IWO45_002789"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:285..287,aa:Phe,seq:gaa)
     gene            329..399
                     /locus_tag="IWO45_002790"
     tRNA            329..399
                     /locus_tag="IWO45_002790"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:361..363,aa:Gly,seq:tcc)
     gene            412..485
                     /locus_tag="IWO45_002791"
     tRNA            412..485
                     /locus_tag="IWO45_002791"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Ile,seq:gat)
     gene            497..568
                     /locus_tag="IWO45_002792"
     tRNA            497..568
                     /locus_tag="IWO45_002792"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Glu,seq:ttc)
     gene            834..2027
                     /locus_tag="IWO45_002793"
     CDS             834..2027
                     /locus_tag="IWO45_002793"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0334220.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2157..3629
                     /locus_tag="IWO45_002794"
     CDS             2157..3629
                     /locus_tag="IWO45_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0334221.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3712..4500
                     /locus_tag="IWO45_002795"
     CDS             3712..4500
                     /locus_tag="IWO45_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0334222.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4567..5235)
                     /locus_tag="IWO45_002796"
     CDS             complement(4567..5235)
                     /locus_tag="IWO45_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0334223.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5350..7062
                     /locus_tag="IWO45_002797"
     CDS             5350..7062
                     /locus_tag="IWO45_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0334224.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            7065..8837
                     /locus_tag="IWO45_002798"
     CDS             7065..8837
                     /locus_tag="IWO45_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0334225.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2669 a         1529 c         1987 g         2720 t
ORIGIN      
        1 gctgaaggga acggtcttga aaaccgtcag gcgggtaaaa ccgtgcaagg gttcgaatcc
       61 cttatcctcc tttttcatat tatcgcggga tggagcagtc tggtagctcg tcgggctcat
      121 aacccgaagg tcgtaggttc aaatcctgct cccgcaatta aaaactaaaa acattggttc
      181 cgtggtgtag gggttaacat gcctgcctgt cacgcaggag atcgcgggtt cgattcccgt
      241 cggaaccgtt tggctcggta gctcagttgg tagagcaatg gattgaagct ccatgtgtcg
      301 gcagttcgat tctgtcccgc gccattatgc gggtgtagtt tagtggtaaa accacagcct
      361 tccaagctgt tgtcgcgagt tcgattctcg tcacccgctt ttagttttta tgggcctata
      421 gctcagctgg ttagagcgca cgcctgataa gcgtgaggtc gatggttcga gtccatttag
      481 gcccatttat tttattggcc cgttggtcaa gcggttaaga caccgccctt tcacggcggt
      541 aacacgggtt cgaatcccgt acgggtcatg gaagatactt tttgagtatc tttttctttt
      601 tatctgaaag aatgatttta aaaagcttag cagagttatc tttatggatg attgtgctaa
      661 gcttttttgg acagttctaa tgtaaaatat ttttatattt tttaagcaaa aaaatgaaaa
      721 aatacgagca gaaaactgtt ttatctgcta tacttggttc gttgcaagtt cccgaaagga
      781 tccgcaggac ggagatgcct tgtaaccgaa tttaactagg gggaatcatt ataatggtag
      841 aaagacactt atttacatca gaatctgttt ctgaaggaca tccagataaa attgctgacc
      901 aaatcagtga tgcaatcttg gatgcaattt taaaacaaga tccaacagca cgagtagcgt
      961 gtgaaacgtc tgtaacaact ggtcttgttt tagtatttgg agaaatatct acgacggcat
     1021 atgtagatat tcaaaaagtc gtacgggaaa caataaaaga aattggttat acacgtgcaa
     1081 aattcggatt tgatggagat acagcagctg tattggttgc gattgatgaa caatctcctg
     1141 atattgctca aggagttgat gaagcacttg agatccggga tgaagataaa aaagacgtat
     1201 tagatgagat tggtgctggt gaccaaggtt taatgtttgg gtttgctgta gatgaaacac
     1261 cagaattgat gcctttacca atcgctttga gtcatcgtct ggtacgacgc ttggcagatt
     1321 tgcgtaaatc aaatgaatta acgtatttac gtccagatgc aaaatctcaa gtaacggttg
     1381 aatatgatga tcaaggacaa ccggaacgcg tagatacaat cgttatttca acacagcatg
     1441 atgatgcagt agataatgaa acaattcgtc atgatgtcat cgaaaaagta gtgaaggaag
     1501 ttattccagc tgaattatta gatgatcaaa cgaaatatta tatcaatccg actggccgat
     1561 ttgtcattgg tggtcctcaa ggggatgccg gattaacagg aagaaaaatc attgttgata
     1621 cgtatggcgg ttatgctcgt catggtggcg gtgctttttc tggtaaagat gcgacaaaag
     1681 ttgaccgttc tgccagctat gctgcacgtt atattgctaa aaacattgtt gcagcagggc
     1741 ttgctcgcaa agcagaagta cagctagctt atgcgattgg tgttgctcaa cctgtttcga
     1801 tctcaatcaa tactttcggt actggaacgg ttccggaaga agaactgatt gctgcagtga
     1861 gagaaaactt cgatcttcgg ccagcaggaa tcatcgagat gcttgatttg cgtcgtccga
     1921 tttacaaaca aacagctgct tacggtcatt ttggtcgtac agatgtagat ttgccttggg
     1981 aacatacgga caaagtagat gcgttgaaag caagtttagc aaaataagtt atattcagct
     2041 ggaacgatcg ccaagtggtg gttgttccag ctttttttat ggcagttaac ctcttgaaaa
     2101 agatagtaat tatttatcta tttttagatc ctgtcataat gaaagggagg aataagatga
     2161 aaaaagaaac aaatgttact ttggtcacag tgagtatttt cattgcaacg ttcatgacgg
     2221 ctattgaagg tacaatcgta acaaccgcta tgccaacgat tgtcggttca ttacatggaa
     2281 tggaaatcat gaactgggta ttttcgattt atttgctgac aaacgctatg cttactccga
     2341 tttacggaaa acttgcggat aaagtgggac ggaaaccaat ctttattttt gggacattat
     2401 tgtttattat cggttcgtta ttttgcggtt tatctcaaag catgattggg ttgattgctg
     2461 ctcgtgcgat tcaagggatg ggtgctgggg caatgatgcc agtagctttg acgatcatcg
     2521 gcgatcttta ttctattgaa aagcgagcaa aagttttagg tttgaatagt tcagcctggg
     2581 ggattgccag tgtttttgga ccgcttgcgg gaggattcat cgttgaaacg atcagctggc
     2641 actggatctt ttttatcaat gtgccgatcg gaattctatt aattattttg atttggctat
     2701 atttaaatga accaaaaatc atacatgagt ctaaaccaat ggatattttt ggtagtttta
     2761 gtttgatggc catgcttgtc tcattgctgt tagggttcca attattagga gacgaaggaa
     2821 tgagtccgct tgtttctgct ttgctgctag gtagtatcgt aatgttaggt atttttgttt
     2881 ttgccgaaaa aagagctaaa gatcctgtca tcatgatgca tttattcaaa aatccactat
     2941 ttgttgtggt caacttggct gcagcattgg ctagtggttt tttgatggga atcgatgttt
     3001 atatccctat gtggatgcaa ggtgtattag ggctgaatgc agcaattggt gggctggtat
     3061 tagcaccatt atcactagtc tggatggtcg gttctttcct tgcaagcaag tggatgactc
     3121 aaatgaaaac tcattgggtc ttgagaattg gactactaat cgcagctatt gggggcgctt
     3181 ggttgtatct tatgcctttc agaagtaact atttatggtt ttttgctata tcaacggtca
     3241 tcggtatagg cttaggtgtg accatgacta cctcgactgt cagtgcacaa aatagtgtat
     3301 ctcgtgacca gcttggtgta gcaacttctt ttaatacgtt agttcgaaca attggtcaaa
     3361 cagtcatggt ttctattttt ggtttattga taaatggggt aactaaacga gaacttgcag
     3421 cagcaaattt aacaaatgat gcagatatca tgaataaact ggtgaatcca caaacggcta
     3481 aatatattga tgcggatatt ttgttaccat taagaaaaat tctttatgat gggttacatt
     3541 ccgtttatct agttggcttg ttactcgtga tcattgcttt ggtcttaacg cttttcgttc
     3601 atgaaaaaag tggtaaaata aaacagtagt ttcggttaag atatgtacgt agccgaatca
     3661 ttcaaattta aagttattca aaaataactt cttactaagg aaggcgaaaa tatggcattt
     3721 attcaagcaa atatttattc gaatgtgtta gaaatggaag tcaatctaaa tgtgatacta
     3781 ccgcaaacaa caaaaaaggt gattggcacc agttcagagc aagagacaac ggatatacct
     3841 gttttatatt tacttcatgg aatgggtggc aatcatagtg tatgggaaag acgtacgtcg
     3901 attgaacgtt atgtagcaaa ctacggatta gctgtcatca tgccatctac tgatcttggc
     3961 tggtacacag atacagttta tgacatgaag tattggacat tcattgcaga agagctacct
     4021 gagatttgtc atgaattatt cccgcaatta acaagaaaaa gagaaaaaac atatgccgct
     4081 ggattatcga tgggcggata tggcgcgtta aaactaggcc ttgcaaaatc agaaaatttc
     4141 gcagcagtaa tctctctttc aggcgctgtt tctgtaggaa atcgaatgga tgatcttttg
     4201 atggttcgta aagcatcttt ttgggaaggg atctttggac cattggatca aatcgaaggt
     4261 tcaaaaaatg atccgcaata cttattgaaa gaactagtaa aaagcggaaa accagttcca
     4321 agattttata tggcttgtgg ggaatctgat tttctttatc aggcaaatca ggaaatggta
     4381 tctgctatgc aggaacaagg cttggcggtc acctttgaac aaggacctgg cgatcataac
     4441 tggatcttct gggaccaatg gattcaacgt gcattggcgt ggctaatgga agaaaaataa
     4501 aaaaagagtc ttaaaaagat agctcctata gagatcacaa acatagtgat ctctatagga
     4561 gcttttttac tgctcgtttt ctcttctttt attttgttgg cataagcctt caatataggt
     4621 ttgtgcactc gttagaaaga gtgctttttc attcaattct gcatgggact gcatactttc
     4681 tttaaatccc tctaattgat tttcaactac ataattacgc ataaatagaa ctgtttcttt
     4741 caaaaattca tttccgtcca taattttttg tcgaagctgc tgttcatgct gctgatccat
     4801 taactgtcca atcaataaat tgtggagaga ggcaatggcc tgatgacttt gggtgactga
     4861 aaatccttca cgaacaagta attggatcat ttgctccaaa tgttctaatc gactcggaaa
     4921 agcgggcaca gtattgattt ctaattctgc cccacaaggg aaacgtgtat aaaggtcata
     4981 gtaattttcc atatactgca gcaactgttc attccaaggc aaagttggat ctggaagcgc
     5041 taattcttct tccattgttt cagccatttt ttgcaagagc tgttgtttgt ttttgaaata
     5101 ccaatataaa gcaggtgctt gaacatgaat tctgtttgcg atgtttcgca tggaaagctg
     5161 cgaaagagct tgtgtttcag ccaggatatc aaaggcggcc tgaatgatga ttgcttgaga
     5221 aagctttatt tccattttat caacctcgtg tattgcatta tctttttaaa catggtaaat
     5281 tgttagtcgg tctaatttta cagtgttaaa ttagtcctgt caatttaaac aaaggggtgt
     5341 aatcaacgaa tgaaattaat cttacgacat gcgaaaaaat ataaagtagc tgtttttatt
     5401 tccttgctgt ccgtagcagt catggttgca gcagcattat ggcaacctaa gttactacag
     5461 caagtattgg aagctattat tacagaagat aatgatgaaa tgaaaacaat tggtatttac
     5521 ctgatcagtc tggctttgct aggattagcc gcaggtgtaa cgaacacgat tttctctgca
     5581 aaagtagctc aaggcgtaag tgcagatatc cgagaagaag catttcgaaa gatccagaca
     5641 ttttcatttg gaaatatcga acagttctct gctggaaatc ttgttgttcg tctaacaaat
     5701 gatatcacgc aaatccaaaa tttagtgatg atttctttgc aatcactttt tcgaattcca
     5761 ttcttattta ttggtgcctt cattttagct atgatcacga tgccgcaatt atggtggatc
     5821 atcgtagcgc ttatcgttac ggtatttctg attacggcac tttcatttac aaggatgggc
     5881 aagcacttca tgatcattca aaaactgatt gatcgtgtga atagcattgc aaaagaaaac
     5941 ttactaggga ttcgtgtagt gaaatctttt gtacaagaag aaaaccaatt gaagtcattc
     6001 agcaaagtaa gtgaggactt aacaaaacac aacatcatag tcggtacatt attttctgtg
     6061 atgatcccgt ccttcatgct ggctgccaat ttggctgtgg ttggggcgat cttttttgtg
     6121 agcgatcttg ttaaagatga tcctacgtta attggtggaa ttgcttcatt catgaactac
     6181 ttgatgcaga ttatgatggc aatcattatt ggcggaatga tgatgatgat gacttcaaga
     6241 gcagccgtat cgctgaagcg gattgcggaa attttagatg ctgaaccaga tcttacttat
     6301 ttagacgttc cagaacaaga gttgacgggt tctgtcactt ttgaccatgt ttcttttcgt
     6361 tatcctggtg aggatacaga tacgcttaaa gatatcagct tttctatcaa acctggagaa
     6421 atggttggta tcgtcggggc gactggtgcc gggaagtcaa cactagcaca attgatccct
     6481 cgtctatttg atccgacaga aggcaaagtt gaagtaggtg gtgtagatct tcgtcaagta
     6541 aatgaaaaga gtttaagaca aacggtttcc tttgttttgc aaaaagcaat tttattctca
     6601 ggaacgattg ctcaaaactt gcgccaaggg aaaaagaatg caacagaaaa agagatggaa
     6661 catgcttctt ctattgctca agcaaaagaa ttcatcgaaa agttatctga tcgttacgaa
     6721 gcaccaatag aagaacgtag cagtaacttt tctggtggtc aaaagcaacg tttatccatt
     6781 actcgtggcg ttatcggtaa tccaaagatc ttgatcctag atgacagtac gagtgcactg
     6841 gatgctcgct ctgaaaagct ggtccgagaa gcactcgatc gagatttgaa gggaacaacg
     6901 acgattgtca tcgcacaaaa aattgcttct gttgtcaaag cagaccgcat cttagtattg
     6961 gatgaaggca gattagtcgg cgaaggaacc catgaggaat tggtagctgg aaaccgtgtc
     7021 tatcaggaaa tctttgaaac tcaaaaagga acggaggagt aagtatgact gatctaataa
     7081 aagcaagtaa atttttctat cactatttga agcggtacaa actctccttt ttctttatct
     7141 ttatcacagt gatcattgct acctatctac aagtaaaggc acctcaatac atcggggaag
     7201 cttttcaaga attagctaat tacgtaggag gcttgatgca aggcgtagac gataaaagca
     7261 aatttgtttc tgtaatatgg aaactcttgc tgttttatgt actagctagt gcagccaact
     7321 ttatctatag tattttgttc acacaagtag tagggaagtc aacgaaccga atgcgtatcg
     7381 gtctattcaa caaattggaa aaattgacga tccgtttctt tgattctcat caagacggtg
     7441 aaattttaag tcgttttacc agtgacttag ataatattca aaatagtttg aaccaagctt
     7501 tgcttcaagt catcacgaat gctgttttac ttgtcgggat cttgatcatg atgttccgcc
     7561 aaaatgtaga gttagcttgg gctacaattg cttccactcc agtagccgtt ttgatagctg
     7621 tcttgattat ccgcaaagca cggaaatatg tggatcttca gcaagatgaa gtggggaaac
     7681 taaatggtta tatggatgaa aaaatcagcg gacaacgtgt catcatcaca aatggcttac
     7741 aagaagaaac gattgaagga ttcttagagc ataatcaaag tgttcgcgaa gcaaccttca
     7801 aaggacaagc ttattctggt ctactatttc cgatgatgca aggaatgtca ctggtcaata
     7861 cagcgatagt tatctttttc ggcggctggt tagcattaaa tggtgatttg gaacgttcgg
     7921 tggcattagg cttagttgtg acattcgtcc agtattcaca acaatattac caaccactga
     7981 tgcagatttc atctggatac agcatgatcc aattagcgat tacaggggca agacgattga
     8041 atgaaatatt tgatgaaaaa gatgaagtca aaccagaaaa cggtaaagca tttgatggtg
     8101 ttgaaaaagg tcttgcgttg cagcacgtag attttggtta ttctccagaa aaactagtct
     8161 tgaaagatgt ctcgatcaat gtggacaaag gagaaatggt tgctcttgtt ggtccaacag
     8221 gatcggggaa aacgacgatc atgaatctgc taaaccgttt ttacgatgtc aatgatggtt
     8281 cagtagtcat cgatggaata gatatccgag attatgatct tgatgcatta cgtagacatg
     8341 taggaatcgt tttgcaagaa tctgtattgt tctccggaac gatcaaagac aatatcacat
     8401 tcggcaaacc ggacgcttca gatgaagaag tcatcaatgc agcaaaacaa gcgaatattc
     8461 atgacttcat catgacgtta gaaaaaggtt atgatacaga gatatccgaa gaaaataact
     8521 tattcagtac aggtcaaaag cagttgatca gtattgctcg aacgatcatc acaaatccat
     8581 ctttattgat tttggatgaa gcaacaagta atgtagacac ggtaacagaa gcaaggatcc
     8641 aaaaagcaat ggatgaagca atcaaaggaa gaaccagctt tgtgattgct caccggctga
     8701 aaacaatctt gaatgcagat agaatcgttg tcttgcgtga tggggaagtc attgaagaag
     8761 gcagtcatca tgagttatta gcagaaaatg gattttatgc ggaactttat cataatcaat
     8821 ttgtatttga aagttaaatt aaaagaacct cgttataacg gggttctttt tatatatttt
     8881 atgttgtttg tttttataag gggca
//
LOCUS       DACEDD010000097         8490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEDD010000097 DACEDD010000000
VERSION     DACEDD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.097"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWO45_002799"
     CDS             complement(<1..173)
                     /locus_tag="IWO45_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0334226.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWO45_002800"
     CDS             complement(593..1714)
                     /locus_tag="IWO45_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ0334227.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IWO45_002801"
     CDS             2042..3451
                     /locus_tag="IWO45_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0334228.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWO45_002802"
     CDS             3448..4176
                     /locus_tag="IWO45_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0334229.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWO45_002803"
     CDS             complement(4304..5215)
                     /locus_tag="IWO45_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0334230.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWO45_002804"
     CDS             complement(5232..6239)
                     /locus_tag="IWO45_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0334231.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWO45_002805"
     CDS             complement(6236..8365)
                     /locus_tag="IWO45_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0334232.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWO45_002806"
     CDS             complement(8370..8477)
                     /locus_tag="IWO45_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334233.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DACEDD010000098         8288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEDD010000098 DACEDD010000000
VERSION     DACEDD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.098"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            221..655
                     /locus_tag="IWO45_002807"
     CDS             221..655
                     /locus_tag="IWO45_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0334234.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="IWO45_002808"
     CDS             678..998
                     /locus_tag="IWO45_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334235.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="IWO45_002809"
     CDS             1139..1330
                     /locus_tag="IWO45_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334236.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="IWO45_002810"
     CDS             complement(1401..1658)
                     /locus_tag="IWO45_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0334237.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="IWO45_002811"
     CDS             1816..2079
                     /locus_tag="IWO45_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334238.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="IWO45_002812"
     CDS             2103..2291
                     /locus_tag="IWO45_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334239.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="IWO45_002813"
     CDS             complement(2374..2559)
                     /locus_tag="IWO45_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334240.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="IWO45_002814"
     CDS             2774..3145
                     /locus_tag="IWO45_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0334241.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="IWO45_002815"
     tRNA            complement(3295..3367)
                     /locus_tag="IWO45_002815"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="IWO45_002816"
     CDS             complement(3413..3841)
                     /locus_tag="IWO45_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334242.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="IWO45_002817"
     CDS             complement(3901..4659)
                     /locus_tag="IWO45_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0334243.1"
                     /translation="MKKKTFKKLLFLGGTTALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4954..5151
                     /locus_tag="IWO45_002818"
     CDS             4954..5151
                     /locus_tag="IWO45_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334244.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5257..5739
                     /locus_tag="IWO45_002819"
     CDS             5257..5739
                     /locus_tag="IWO45_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334245.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(5797..6393)
                     /locus_tag="IWO45_002820"
     CDS             complement(5797..6393)
                     /locus_tag="IWO45_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0334246.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            6779..8245
                     /locus_tag="IWO45_002821"
     CDS             6779..8245
                     /locus_tag="IWO45_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0334247.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT         2713 a         1313 c         1635 g         2627 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagttgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat attttaatat
     4741 gtaggaaata attagttttt aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa
     4801 tacaacagct gttttaattg tgaaaacgaa aacagtacgc tataatgcct gtaaggttga
     4861 tttttaactg taaataaaaa tcaatgagtt aaatctccaa agaaatagca gtggtaatgt
     4921 ggagaaaagt tccagcagtt taggaggatt ttgatgacgg aagaaatgat taatttagga
     4981 gaacaatatg catgcaaacc aattggtttt acaaaaacgg tcattggtga agttgtatca
     5041 aaaatgacaa actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta
     5101 ttagatgaaa aagcaagcat ggttgttgca aaatatgata catttgaata aagaaaacaa
     5161 ttggacgtgc ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag tattcttttc
     5221 ctattttctg gagaagaaaa agcgaggcga gttgtcatgg taacattctt tacggttcta
     5281 ttgtttttat tcgttgttgt gatgttttta tttctttggc tggttcgaaa agaaattatt
     5341 tatcggactg tgcgaaatcg atgggtctac ttggtgatac ccttcttagt gtcactagtc
     5401 atttggtata cactcatcag tcatcctaca gcagatgaat tggcaaaagg gatcttgtca
     5461 gcaatgattt ttattagttt tttgttggac agtagaggaa tcacagaaga aggacttgtt
     5521 ttgaatagct ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat tgtattatat
     5581 cagccaaaag gttctaaaat cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg
     5641 atgctgaaat tttctgcatc attagaagaa ttagttccat ttttgtccca acgcttaaac
     5701 gatgaggcag agattgatat tatgattgat ccagaataaa aacagaggtt gcgacaagat
     5761 gtttgtcaca acctctgttt tttatttcaa ataaaattat ccaaatacgc ggaagcgatc
     5821 ggcatcgttc atgtattctt tttccccagc cggtgtttct ggtgaaccga atactaattg
     5881 cgaacgtagt ttccagtttg aagggatatt ccattctttt gcaactgctt cgtcaataac
     5941 tgggttgtaa tgttgcaagt ttgcgcccaa tcctttatca actaatgcta cccaagtatt
     6001 agctgttgcg attccgtttg attgttctga ccaatctggg aagttgtctg catataattc
     6061 aaattgttct tgtagacctt ttaccacgtc agtatctttg aagaataata ctgtaccata
     6121 accatttttg aaaccagcta atttgttttg tgtatttggg aaagcttctg ctggagtcaa
     6181 tgggcgaagt gcttcttcag tgatttccca aagtttttca tgtgcatctc cgaaaaggat
     6241 aactgcgcgt gttgattgag cattgaaagc agttgggctt tctttaattg cttctttgat
     6301 caaagttgtt aattcttcat tagaaagtga aacattacgt cctagatgat agattgaacg
     6361 gcggtttttc aaagtgtcag taaaagttgt cataataaaa tttctccttt gatttgcgtt
     6421 ttgcattttt ttaattgatt acgaaatgaa gtatagcacc ttacaaaaag taagtaaaca
     6481 gatatgctca caaaaaataa gcgaagttca aaaaacaatt ttaataagaa aaatcgttat
     6541 gtaaggattt tcttagttat cgctttcatt gttgtgtttt aggaattttt gcttatttga
     6601 aaaaaacagc tgatatataa ttattaaatc tctatcttct ttttttatga tattgccatt
     6661 aaaaattact tgcttttata gaaaattagt ttatatttct taaataagtt ttttagttaa
     6721 atttttcggt aaagatgtat aatattttct gaaaatttaa agaattgggg tatagattat
     6781 ggaaaaacag caagacaagg gcttcttcgg gcagccgaag ggattgtcaa ctttgttctt
     6841 cactgagatg tgggaacgat ttagttatta cggtatgcgt gcattactta tctattatat
     6901 gtacgataca gttgccaacg ggggacttgg attaccacga gcaacagcat tagctatcat
     6961 gtcgatctac ggatctttga tctacatgtc tagtatcgtc ggcggatggg tatctgaccg
     7021 agtgttagga gcaaaacgca ctgtattctt cggtggagtt ttcattatgc ttggccacat
     7081 cgtcttggct ttaccatttg gtgtaagtac attgtttgtt tcgatggcat tgattattat
     7141 cggaaccgcc tttttgaaac caaatgtttc tggcatggtc ggacatcttt attcaaagac
     7201 tgacttgcga agagatgcag gattttcgat tttttacatg ggtgtcaact taggggcatt
     7261 attagcacca ctaatcgttg gtacaatcgg tcaagaatat aactatcatt tagggttctc
     7321 tttagctgct attgggatgt tctttggatt attacaatac atcatccaag gacgcaagac
     7381 attagacgga gtgggtacaa caccgccgaa tcctttgaca actgaagaac gtaaaggatt
     7441 catccgtaac ttgatcatcg cacttgttgt tatcgctgca gtattcggcg gggcacaact
     7501 aactggtcat ctgacaattg acttttttgt caatacaatc agtgtattgg ggattatctt
     7561 accgttgtat tactttatca aaatgctgac ttcaaaagat gtaactgctg aagaaaaacc
     7621 aagagtatgg gcttatatcc cattattcct tgcagcaatc attttctggt cattacaaga
     7681 acaaggttca tctatcttag ctttatttgc tagtgaacgt acacaatcta cgctatttgg
     7741 tgtaacgatc caagcaagct ggtatcaatc attgaatcca attttcatct ttattctgac
     7801 accagtattc gtaacgatgt ggacgaaact tggtgaacgt caaccatcta cagtagtgaa
     7861 attctctctt ggacttttat ttgcaggatt atcgtacttg ctattgatgt tgccgggtat
     7921 gctttatggt acaagtggaa aagtaagtcc attgtggttg ttgggaagtt tcttcatcgt
     7981 ggttattgct gaattgtgtc tttcaccagt tggtttgtct gtgacaacaa aattagcacc
     8041 acgagcattt gaatcacaaa caatcgctat ttggttctta gctgatgctt cttcacaagc
     8101 aatcaatgca caaattgcac gcttctacac accgggaaca gaatctgctt acttcggtat
     8161 tatcggtgca gtagcaatcg tcggtggtat cttgctattt actgtgaaag aaccaatcaa
     8221 aaaactaatg ggctctattc attaatcaat gaacataaaa acaggaattg cttatttgca
     8281 gcagttcc
//
LOCUS       DACEDD010000099         8251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEDD010000099 DACEDD010000000
VERSION     DACEDD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.099"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IWO45_002822"
     CDS             <1..197
                     /locus_tag="IWO45_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334248.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IWO45_002823"
     CDS             314..811
                     /locus_tag="IWO45_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0334249.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IWO45_002824"
     CDS             898..1290
                     /locus_tag="IWO45_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0334250.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="IWO45_002825"
     CDS             1274..3721
                     /locus_tag="IWO45_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0334251.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="IWO45_002826"
     CDS             3724..5907
                     /locus_tag="IWO45_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0334252.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="IWO45_002827"
     CDS             5904..6905
                     /locus_tag="IWO45_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0334253.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="IWO45_002828"
     CDS             6920..7834
                     /locus_tag="IWO45_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0334254.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="IWO45_002829"
     CDS             8079..8195
                     /locus_tag="IWO45_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0334255.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DACEDD010000100         8066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEDD010000100 DACEDD010000000
VERSION     DACEDD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.100"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..884
                     /locus_tag="IWO45_002830"
     CDS             <1..884
                     /locus_tag="IWO45_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0334256.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="IWO45_002831"
     CDS             932..3061
                     /locus_tag="IWO45_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0334257.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIESVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="IWO45_002832"
     CDS             3036..3725
                     /locus_tag="IWO45_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334258.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="IWO45_002833"
     CDS             complement(4133..4819)
                     /locus_tag="IWO45_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0334259.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="IWO45_002834"
     CDS             complement(4955..5149)
                     /locus_tag="IWO45_002834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334260.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="IWO45_002835"
     CDS             complement(5199..5639)
                     /locus_tag="IWO45_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0334261.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="IWO45_002836"
     CDS             complement(5643..6488)
                     /locus_tag="IWO45_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0334262.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="IWO45_002837"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="IWO45_002837"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0334263.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2479 a         1514 c         1714 g         2359 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga atcggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaa
//
LOCUS       DACEDD010000101         7976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEDD010000101 DACEDD010000000
VERSION     DACEDD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.101"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(3..1847)
                     /locus_tag="IWO45_002838"
     CDS             complement(3..1847)
                     /locus_tag="IWO45_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334264.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2059..2577
                     /locus_tag="IWO45_002839"
     CDS             2059..2577
                     /locus_tag="IWO45_002839"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0334265.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2705..3388
                     /locus_tag="IWO45_002840"
     CDS             2705..3388
                     /locus_tag="IWO45_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0334266.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3697..3770
                     /locus_tag="IWO45_002841"
     tRNA            3697..3770
                     /locus_tag="IWO45_002841"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3731..3733,aa:Arg,seq:tct)
     gene            3929..4288
                     /locus_tag="IWO45_002842"
     CDS             3929..4288
                     /locus_tag="IWO45_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0334267.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4411..6042
                     /locus_tag="IWO45_002843"
     CDS             4411..6042
                     /locus_tag="IWO45_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0334268.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6104..6745)
                     /locus_tag="IWO45_002844"
     CDS             complement(6104..6745)
                     /locus_tag="IWO45_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0334269.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7027..7335
                     /gene="rplU"
                     /locus_tag="IWO45_002845"
     CDS             7027..7335
                     /gene="rplU"
                     /locus_tag="IWO45_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0334270.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7362..7706
                     /locus_tag="IWO45_002846"
     CDS             7362..7706
                     /locus_tag="IWO45_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0334271.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7719..>7976
                     /gene="rpmA"
                     /locus_tag="IWO45_002847"
     CDS             7719..>7976
                     /gene="rpmA"
                     /locus_tag="IWO45_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0334272.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKK"
BASE COUNT         2529 a         1320 c         1638 g         2489 t
ORIGIN      
        1 ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc aatgaaagta
       61 tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat catcattaag
      121 tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag gtttgtatcg
      181 taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa
      241 taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga atgctctaaa
      301 gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc gggaagatct
      361 gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg cgctaagata
      421 agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt tgcttcttct
      481 aatatctttt ccacatagtt tttagctttc gtttggtccg tatgcattaa atcttgcaga
      541 ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat atgctgtcga
      601 agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc tacttgtctc
      661 cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat aacgattaga
      721 atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc taatgccgtt
      781 tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt acaagctaca
      841 taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt
      901 ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg agtaaaagtt
      961 gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat
     1021 tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga cgttaagata
     1081 ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc acttaaaata
     1141 tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag aataagtgag
     1201 tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt ccctatagcg
     1261 ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata aataaaacta
     1321 atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata gttctgataa
     1381 ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa atctatcaca
     1441 gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta taaaatcttt
     1501 tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc
     1561 aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac ggtaggaatc
     1621 gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata gaaaacccac
     1681 gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa ataaaagaaa
     1741 taagcaattc ctgttaaaaa taacaatgat aatataatga ttatgctcca ctttatacgt
     1801 aaataaaaag attctttttt attatatatg ttattaaaaa aagacataaa ggtattcctt
     1861 tctaaaactt gtaatgttag ggtaaacgtt ttttaaacta acgtcaatta tttataataa
     1921 aaaatgagat aaagatgagg aaatagaatt agaaacatat aattattaaa gggaaacatt
     1981 tttacagcta taatttgaaa aaaatttatc gttattatat gattaagaag gattgaataa
     2041 agaaagaagt tgaaaacgat acctgattac aaaaaaataa cttttataag ttctataacg
     2101 ttgattatat ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg
     2161 actccttata ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata
     2221 tatgtacgac cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa
     2281 aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat
     2341 ttttatacaa aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat
     2401 attatcggta gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca
     2461 tttgcatgga ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca
     2521 tggaatacct ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa
     2581 tgttaaaaac ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga
     2641 aaacactcat gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag
     2701 tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat
     2761 gcgtacccag caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt
     2821 agcagaagta ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca
     2881 gcttcagcct gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat
     2941 ggccacgaca atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat
     3001 cgagaaagct aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga
     3061 aggtcaagca gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga
     3121 tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg
     3181 tggcgttttg atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga
     3241 gattccccgt ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat
     3301 ggatcatccc gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac
     3361 aaaggaatca gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat
     3421 tgaatcgagg gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa
     3481 ggatacagga aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa
     3541 ttgataacct tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt
     3601 gggccaaaat aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt
     3661 taattatcta taattattga tgttccgctg attcttgtca tagtagctca gctggataga
     3721 gcatccgcct tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc
     3781 gttagaaact gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt
     3841 caaaggcaaa taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat
     3901 aaaactcatc agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct
     3961 agttgcaatt gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac
     4021 gacaagtagg gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt
     4081 gtttaagaac caaggagtgt acaatggact attaggagtg ggactacttt atggagctta
     4141 tctttcttcc gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc
     4201 attatatggc agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct
     4261 gttggcattg ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga
     4321 aactttgtga aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt
     4381 agaaaatttc atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa
     4441 aaagaatttt caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca
     4501 gtagctgctg tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg
     4561 gccgcaggtt tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt
     4621 ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca
     4681 acaaagggaa tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca
     4741 gcaggacttt tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg
     4801 tttacttcag gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt
     4861 cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg
     4921 ataaacacag cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca
     4981 aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt
     5041 atgctattgt tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt
     5101 agtaatcgtt tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca
     5161 gctattagca ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc
     5221 cgaatggcaa ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat
     5281 ctgttgcttc ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg
     5341 gccatcaaat ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg
     5401 ctctccatgc ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt
     5461 gttttgattg tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact
     5521 aataaatatt ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt
     5581 gatttaagta ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa
     5641 agcgctgcta tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa
     5701 acggaaaagc tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct
     5761 tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc
     5821 atgcgaggcg taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa
     5881 aaagcagagc aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa
     5941 caactttctc acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg
     6001 ttgacagctt tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag
     6061 acaagactgt aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa
     6121 gtgaagctag tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa
     6181 tagctgctgg atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat
     6241 ccttttgttc tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa
     6301 ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt
     6361 ttggctggac cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac
     6421 gagtaggtgt ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat
     6481 gagtcacgcg atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta
     6541 agccagttga agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat
     6601 gcgtatcaat ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag
     6661 gtccttgccc agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt
     6721 gtatcagttc ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa
     6781 aagaaacaca atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat
     6841 tgcttgaatt tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct
     6901 ggtaagctag tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat
     6961 aagcacagca tatgctgtca cttcggatgg cgagtctaag tatatgagaa ggaggtgctt
     7021 tatagcatgt acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa
     7081 gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt
     7141 atcttagtag gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt
     7201 gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct
     7261 aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac
     7321 gcgatcaacg cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta
     7381 agcgaaatga cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc
     7441 cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg
     7501 ggattgcctc attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt
     7561 atctttatac cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc
     7621 tattagaaaa tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc
     7681 aaattaaaac aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac
     7741 aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa
     7801 aacgtctagg tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc
     7861 gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt
     7921 tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaa
//
LOCUS       DACEDD010000102         7928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEDD010000102 DACEDD010000000
VERSION     DACEDD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.102"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(144..524)
                     /gene="yajC"
                     /locus_tag="IWO45_002848"
     CDS             complement(144..524)
                     /gene="yajC"
                     /locus_tag="IWO45_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0334273.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(621..1766)
                     /gene="tgt"
                     /locus_tag="IWO45_002849"
     CDS             complement(621..1766)
                     /gene="tgt"
                     /locus_tag="IWO45_002849"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0334274.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1984..2682
                     /locus_tag="IWO45_002850"
     CDS             1984..2682
                     /locus_tag="IWO45_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0334275.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2850..5489)
                     /locus_tag="IWO45_002851"
     CDS             complement(2850..5489)
                     /locus_tag="IWO45_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0334276.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6108..7139)
                     /gene="queA"
                     /locus_tag="IWO45_002852"
     CDS             complement(6108..7139)
                     /gene="queA"
                     /locus_tag="IWO45_002852"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0334277.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7332..>7928
                     /locus_tag="IWO45_002853"
     CDS             7332..>7928
                     /locus_tag="IWO45_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0334278.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         2242 a         1717 c         1458 g         2511 t
ORIGIN      
        1 agttatttat tattatttat atgacacacc cgattattca atattgagca atatatccca
       61 tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc tgccctacct
      121 cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc ttccagcgtt
      181 tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt ttcaacagta
      241 gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc tgcacgatcg
      301 tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt gtcgatttct
      361 gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt catgctatct
      421 aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa aaaccacatt
      481 gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt cacctcacta
      541 attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag ttttttactt
      601 aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa cccgtattct
      661 tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg acgtacttgt
      721 ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat cccaaatgtt
      781 tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca tgtataacaa
      841 tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt cactactaga
      901 cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa gacacagtca
      961 aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc gacacccatc
     1021 aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac acgattcatt
     1081 tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa atccatcccg
     1141 actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg gatgattcca
     1201 aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc ccatcgagac
     1261 gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg acattcgtca
     1321 aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt ttctggtgat
     1381 aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc ttcgatattc
     1441 cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat cggttgatcc
     1501 cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc agggcgtagc
     1561 cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa ctcttctggg
     1621 gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt ggggaaagtt
     1681 ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt cttgattaaa
     1741 cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa atatcttgac
     1801 aactcccacc atcataatcg tttcaagtgc tttttgcaag ttccctcggc ttccttctac
     1861 tttatttaat attctttttt ttatcctttt tttatgaata acaaaaaaaa gaaaaacatt
     1921 atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa ggagggaaaa
     1981 atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa aggacgttgg
     2041 ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac tattcttctt
     2101 attatgattc ctattatctt gtttgccacc accatatatg atggatcagc gagcgtctca
     2161 gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat ttccagcatc
     2221 attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt acgtggacaa
     2281 aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt gtttatcggc
     2341 ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg tttgctatta
     2401 gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat ttactacgat
     2461 atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc aagtcgaaaa
     2521 ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat cggctggcat
     2581 atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata catttctgca
     2641 acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt gtgacttgtg
     2701 accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa cattcagccc
     2761 attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta tagttccagt
     2821 aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc cggtaatcga
     2881 tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc taccaagaac
     2941 cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg cagcaaagca
     3001 acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt attggtcgct
     3061 aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag aatcgatgaa
     3121 taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc tacccatggg
     3181 ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat ggtgaagacc
     3241 cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc atttttcgga
     3301 atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc aaatcctgga
     3361 agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg tgctccccat
     3421 cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct tagcaaaaag
     3481 ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc gtctacgatc
     3541 gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac atctgttcct
     3601 gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc attcacacca
     3661 tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat ccggatcttg
     3721 tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc taattcttcg
     3781 tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt caagatacct
     3841 aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat gactgttttg
     3901 atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg tggatcgatc
     3961 attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga aatatcttgc
     4021 ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg tttaccaaag
     4081 cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg agcaagaaca
     4141 tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac tgttgtcatc
     4201 atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc ttctaattct
     4261 gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt tggattacct
     4321 ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag tgcagccatt
     4381 ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac ttcatcttct
     4441 ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt tttgtctgag
     4501 cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat gatcgcatgt
     4561 ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac agtcaaggtt
     4621 tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag gagtggttct
     4681 ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat catactgatt
     4741 cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt attttcgttc
     4801 aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac gatcgctttc
     4861 ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc acctaatgac
     4921 tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc tccagtcaac
     4981 gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc agaaatagca
     5041 tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc aacagtggtc
     5101 actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa ggattccagt
     5161 gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac aacgatagcg
     5221 atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt ataaaaggaa
     5281 atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg aaagaatttt
     5341 ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt ttttcgccgt
     5401 tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac cttctttact
     5461 tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct tcgtacaaaa
     5521 aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa gggcttatcg
     5581 attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc taccaaagga
     5641 tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga atttctcgat
     5701 agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac atgcgtgtag
     5761 agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt atccgaaaca
     5821 agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg aataggaaca
     5881 agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa catgaagaaa
     5941 ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt tatttgaatc
     6001 cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt gtcataaaat
     6061 ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta tttcacgaac
     6121 atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg gtaagcagat
     6181 aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga ttttggcaaa
     6241 tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt aatgaaaata
     6301 tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat cgtttctaat
     6361 gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg tacttggttc
     6421 aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg gtgttcttca
     6481 attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt cagatagact
     6541 aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg caatccagct
     6601 gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg atctgggtct
     6661 tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc aagaatctct
     6721 agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa ttcttctttc
     6781 actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt ggcacgtttc
     6841 gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag aaggacttct
     6901 aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac tcgtgtatta
     6961 ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa atgtttatct
     7021 tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg gtcttttaat
     7081 ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt tgttaacata
     7141 tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt tctgttggca
     7201 aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact accctatttg
     7261 tattttttca ttgttttagg catactagta atagatagtt cttcattgaa aatagaaaaa
     7321 gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt ggaaaagtcg
     7381 ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt atcggtacga
     7441 gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat ccaacacaag
     7501 ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa ttgcgacgtc
     7561 agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc cgtgaaaata
     7621 tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa acagctgaaa
     7681 aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca catgaattat
     7741 ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat caagatatca
     7801 tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc ttgcaagacc
     7861 ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc catgacatgg
     7921 atgaagcg
//
LOCUS       DACEDD010000103         7598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEDD010000103 DACEDD010000000
VERSION     DACEDD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.103"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(136..426)
                     /locus_tag="IWO45_002854"
     CDS             complement(136..426)
                     /locus_tag="IWO45_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334279.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IWO45_002855"
     CDS             complement(544..804)
                     /locus_tag="IWO45_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334280.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IWO45_002856"
     CDS             complement(941..1354)
                     /locus_tag="IWO45_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0334281.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IWO45_002857"
     CDS             complement(1810..2295)
                     /locus_tag="IWO45_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334282.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="IWO45_002858"
     CDS             complement(2434..2649)
                     /locus_tag="IWO45_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0334283.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IWO45_002859"
     CDS             2873..3673
                     /locus_tag="IWO45_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0334284.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IWO45_002860"
     CDS             3692..5560
                     /locus_tag="IWO45_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0334285.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IWO45_002861"
     CDS             5553..6044
                     /locus_tag="IWO45_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0334286.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IWO45_002862"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IWO45_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0334287.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="IWO45_002863"
     CDS             6604..>7598
                     /locus_tag="IWO45_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0334288.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACEDD010000104         7505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEDD010000104 DACEDD010000000
VERSION     DACEDD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.104"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            75..1216
                     /locus_tag="IWO45_002864"
     CDS             join(75..303,303..1216)
                     /locus_tag="IWO45_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0334289.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1451..1903
                     /locus_tag="IWO45_002865"
     CDS             1451..1903
                     /locus_tag="IWO45_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334290.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1917..2606
                     /locus_tag="IWO45_002866"
     CDS             1917..2606
                     /locus_tag="IWO45_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334291.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2731..5082
                     /locus_tag="IWO45_002867"
     CDS             2731..5082
                     /locus_tag="IWO45_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0334292.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5162..5557
                     /locus_tag="IWO45_002868"
     CDS             5162..5557
                     /locus_tag="IWO45_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334293.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5882..6499
                     /locus_tag="IWO45_002869"
     CDS             5882..6499
                     /locus_tag="IWO45_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0334294.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6540..6839
                     /locus_tag="IWO45_002870"
     CDS             6540..6839
                     /locus_tag="IWO45_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334295.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6873..7040
                     /locus_tag="IWO45_002871"
     CDS             6873..7040
                     /locus_tag="IWO45_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0334296.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7085..>7505)
                     /locus_tag="IWO45_002872"
     CDS             complement(7085..>7505)
                     /locus_tag="IWO45_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0334297.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2589 a         1130 c         1501 g         2285 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgt agaatctaac tactaagaaa
       61 aggacgtgtc cacgatggca aaagccaaga aaaaatttga tgaagacttt aagaagatga
      121 tcttagattt aaaccagtct ggccaatcgg tcgaagaact ggcagcgcaa tatggtatcg
      181 ctacacaaac gatttatcgt tggaagaagc ttcatacaaa gaatgtagca accggcatga
      241 ctgaggcaga aatcctagcg atgaaaaagg aaatggcccg catgcaggag gaaaacacta
      301 tcctaaaaaa ggctttaacc atattcgctc aaaagtaaaa atgagtgatg tcttcgaatt
      361 tattcaatca aatgttcatg atcatgacgt aaaacaaatg tgtaccgttt tggaagcacc
      421 gaaatccagc tactatgact ggaagaaaaa gaaaccttcc aagcgccaat cagaaaacaa
      481 acgcttaaac cgcttgattt caggaatcta ctttgaaaac aagggtatct atggtgcgcc
      541 taaaattcat aagattcttg ttggccgtgg cgagaccttg tccttaaaga aagtacaaat
      601 actgatgaga aaattaggct tgcgttcagt caccctcaag aagtacaagc ctggcaaaca
      661 agcgaaagtt aaaagtgaag gacgtaaaaa cctgttaaac caagatttta ccactacgtc
      721 gatcaatcaa aaatgggtga cagatattac ctatattcat accctaaaag atggctggac
      781 ctacctctcc actattcaag acctccacac aaagaaagtc atcggttgga agtttggcaa
      841 gcagatgacg aaagaattgg ttatcgaaac ccttgacaat gcgctcttaa accaaacacc
      901 ctccgagaac ttgatcatcc actcagattt gggttctcag tacactagcg aagcctatga
      961 ggcaaagctg aatgaattga acattcggca ctccttcagt cgtaagggct gtccctacga
     1021 taatgccggt atcgaatcgt ttcacgcctc actcaaaaaa gaagaagtct atccatcaaa
     1081 agcgtatgaa aactttgaag ctgcttattt agcgttattt caatacattg aaggatttta
     1141 caatcgcaag cgaatccata gtagtatcaa ctatttaaca ccaaatcaga tggaggagtt
     1201 ggcattgcaa gcttagttcc tagctaccac ttacattttt ttggcgagat ttccacaggg
     1261 tttcaacttc atcgcttcga tcagcggtca aacggttttg gtttgaccgc tggtcgaaac
     1321 gatgagatac tcacccgctg gaatctcgaa aaaattctca caaatcgtgt ccgaggtatt
     1381 gactcaaatc caccaattat cttatcgtag atatatatta taaatttaat aagaaagcga
     1441 ggtgagaatc gtgaaaaaaa cgatactaag tattttttca gtatttacaa tattaattgg
     1501 gggctatttt cttttgaatt tagattctgt taattcaaac gctcaaataa aagatcaaag
     1561 agtagctgaa cataataata aaaaaatgag tttgaccaaa aatatctatt ctatgtcaga
     1621 tgaagaattg tataaagaat ttggtgattt atccggggaa cagttagaag aaagtattcc
     1681 aggaattagt gactcagata aaattgttgt cttgtctgct ggtgggcaat taatgggaga
     1741 tggcaaatcc gaattagtta gttataaaca aaatcacaga cagtttattg atgttaatac
     1801 tgattccaac gctatttcga ttaaggatct aaaagagctt ttaaatagta aaagaaatga
     1861 attaaagcag ataaaagaaa ctaattcagt ggaattagag taaaggaaga ttcattatga
     1921 aaaagatttt tggtgtttct gttgggctac ttatgatgtt aacatttgga aatgcagtat
     1981 atgctgctga gaaagaagtt atttcagatc cagagcagta cgcctcacga tactttgctg
     2041 attttgaaga agaagataaa gtacttgcat tgccagaagg tggtttttta aaaggggaag
     2101 ctaaaattgt atctatggat aatccagata taattcaagg ttcatataat tcagaaactg
     2161 atcctgcttc agtttcagtg acagaagcaa aaaaaattgt tgctgaaaaa ttcaaaaatg
     2221 atattgaaac tactccaatt atgcctcgag gagcatttcc acccgatgtt ggtactagcg
     2281 gacaccttct tgtactagca ggaggaggaa gctataaatc acagccattt agtggtgcgg
     2341 gatggcgttt tggaggtatg ggtgtaaaac cagctgatgg tacaggcaac tttttacgtt
     2401 ggaaatccta cattgacgat ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat
     2461 ctggttctat tcaaggaatg gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa
     2521 acgggcctgg ttggacaatg aatataggta tttattacac ttataatcct gttgcaggta
     2581 cgtattatgt tgttcaaaat atttaataaa acagcacaaa gagatttctt tgtgctgttt
     2641 tattattttt agttgttgac aaacactaaa atataaaata taatcaacac ataaacgaac
     2701 gtatgttcgg caagaaagca ggggattttt atggatgaga tggttttaag tactcagaaa
     2761 tggctaaaca agaaatacag taatgttaca gggtttgata aggttccaga aaatgggcga
     2821 acagggtggc ctacaatcta tggacttatt gaaggactac aagtagaact tggtattact
     2881 aatctagtag ctaattttgg acctaccact gaaaaaatgt acgataatca ggttactcct
     2941 aaatggggca aaaatctacc taaaaatatt gtatttctta ttcaaggtgc cttttggtgt
     3001 aaggggatta atccaggtgg cttcgatggg gtatatactc catatttaga tactgctgta
     3061 aaagagttgc agactgatgc agggtttaat ggaagcgctg taactggagt tttggatgcg
     3121 aaatgggcca aagcgttatt tgatatgagt gcttttgttc ttgttcccgg cggagatagt
     3181 aaaattagaa aaatgcagca atggttaaat gtaaattatt tagaatacac gggaataatg
     3241 ccttgtgatg gtatttatca aagaaattca aatcaagcat taatttttgc attacaagct
     3301 gaactaggat attctcccag tgaagcaaca gggtcttatg gtaatgggac aactagtaaa
     3361 acttatcctg tgtctgaagg taactcggga aactatgtaa gaataattca atatggatta
     3421 tatgttaatg gttattttga agacggtaca ttcgatggta ttttcactaa atatatgggt
     3481 ttagaagtac tggcattccg aaaatttatg gtattgcctg aatatactga aatagcagat
     3541 gttgttgtaa taaaaggact attgtctagc gctggagata ttagaagatc tgcagatggc
     3601 gcagatactt ctacgcaatt aactagatct caaatccaaa cattggttga taatgatata
     3661 aaaatagttg gtagatatct tactggtact gttggaattg gaaaagatga acgaaataag
     3721 tatctaacta cagaggaatt gaataatatt ttttctaaaa atttatctgt gtttcctatc
     3781 tatcaagatg gaggtgcagc attagcttat tttacttatg atcgtggctt atcagatggt
     3841 aagaaggcta tagatgcagc aaaaaatcta aatataccat taacaacagt tatttatttt
     3901 gctgttgatc tagacatgtt aggggaagaa attcttgcat atgctggaga atattttaag
     3961 ggggtatctg ctgtaatgtc atatagcggg tatcaaacgg gtgtttacgg aacaagaaat
     4021 gtttgttctc aaataatcaa taacgggtat gcttcgtttg catttgtttc agatatgtcg
     4081 actggttata gtgggaattt aggttttcct atgcctagac aatggtcttt tgatcaattt
     4141 gttgaattta ccataggaag tggcaacgga gctgttggta tcgatttaat cgctacatcg
     4201 ggtagagata gcggatttaa tgagttatca aatgatacta ataatgataa ttacattgcc
     4261 aaatacaatc agaaggttat aaatcagatg gtacgtgcgt atcaatatct atcacaagct
     4321 ggtcttgata atccttggaa ccctcatttg acattttata gatatgtaaa ttattctggt
     4381 ttgtcatggg acatcatttc ttctcctgta acagagcatg atagaaaaat ttatgatgaa
     4441 tataggaata aattaactaa tagcgagggc ctgtataatt attttataga tcctaatact
     4501 ggttcaataa taggtttgcc tcacttaatt gtgacgttac aatcacagat gtttctagcc
     4561 gacacaatca atgatagtgt ttctgacttt gcaggttggt taggagattt aatgacttgc
     4621 tggggtgaag ttaagaaatt aggaatacaa atggagcaag gtgtatttgc attagtaggt
     4681 acggctgcag gtggaacatt tagtctagaa gatttatacc aagatgttga cgcggtaaat
     4741 gtttatacct tagcaaaaag aaattctgat tcaacagctt tatcggcttt aaaaacgtac
     4801 tattgtgata agggtgctat tgatagattt aatcaatttg ttaagaacag atttggttct
     4861 tcagataaaa tcgcttcagt aacaaatagt ttattagaaa atgacgaaac agttgatatc
     4921 ttaggtgtag ggataatttc atttttcatt gataaaatag ttaataattc acttgggaca
     4981 gattttactt atcttgattt tattggagat gaggggaaag ttgcaaatgg attttctgaa
     5041 aaaattattc aatttcaaga aaatagaagt aaacttaatt aagaatcgtt tactatttaa
     5101 acagtgtcct tttccttttt aaatatatac gataaaataa gtatatgaat tgtaaaagga
     5161 catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata aaaataattc
     5221 tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac ttttaataac
     5281 tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc
     5341 tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag taaaaaaatt
     5401 aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg acttcaaaga
     5461 aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa
     5521 ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa attgtaaaag
     5581 ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa
     5641 actggataac tagtttattt aatggcagac cgttaaggcc tgccattttt atgtatttta
     5701 gcggaataaa tataatcatg cgggagtgta aacttactgt ttcaaaaagt atactttttg
     5761 acatgccttg attttctttg tttatgtatg gcataataac cacataatac aatttgaaac
     5821 gtttctgatt taatctgatt gttttgaaac aattttagtg aaaaaagggt agataaaaac
     5881 gatgagaaaa tttttttata tccgggtcag tagttatgat caatcgatcg aacgacaact
     5941 gatttccgcg aaggaattag gaatccctga agaatatata tttgtagaaa aagccagcgg
     6001 caaagatttt aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa aaggagattt
     6061 actctatcta caatcattag atagactagg cagaaataaa caaatgattt tagaagaatg
     6121 gcgagaatta actaaggaaa agcagattga tatttctgtg ttagacatgc ctctgttaga
     6181 tactgctagt tacaagcaac tagatgggtt agagacgctt atctctgact taattctgca
     6241 attactcagc tatatggccg aagacgagcg gaaacgaatt aatagccggc aaagagaagg
     6301 cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg ctttacctcc
     6361 tgaatttcct gcgatctatt ctgactggaa agcaggaaag ataactgcag tctatgctat
     6421 ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat atgaacatac
     6481 cttgcaccac ttttcttgat agatataaca gctattagac gggaagggag agtttgaaaa
     6541 tggacttaac gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg acacgaaccc
     6601 aagccatcgg tttggttcac tctttaaacg gccattgtca atcaagtgtt acttcaaaaa
     6661 caaatttcct ggtttgtggt cagtattcta agtcgttgtt tgatgattct ctctatacaa
     6721 aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca tgagattacc attttatcag
     6781 aagtcgaatt ttatgcgtta attagtcaac agttgaaaga atggcaacgg tatttataat
     6841 cgttagtaaa atttacaagg aggaaaattt ttatgaagcg aaaatcttat gatcgttggg
     6901 gcattgaaat aactagtttt aatcgaaata ttgcttttca cgtggagtgt gactgtgggg
     6961 aattagcaaa aagtgtttat caaaaaaatt acttttactg tcctaaatgc catcgccgat
     7021 acggggtaca tggaacatag aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat
     7081 atttctacaa atcattcgct ttctttattt tataaatagt tggtcgagag atcccgtttt
     7141 cttttgcaat ctctgcaact ggaatgcctt gctttaacat gtccaccacg gttttaaaaa
     7201 ctaagcgttt ttgcggatct gttgagttaa tagaatactc gattggtcgt cccttatatc
     7261 ggccggcttt ttgagccaat aatactcctt gccgctgacg ttcacgaatt ttctcacgct
     7321 cgttttgtgc aatataactt agcagtgata aaaacatatc gaacatagcg gtatccagga
     7381 gttcatttcc agtattaaaa tctaaaaact gtgcatctaa tatttttaac gctacttttt
     7441 tctgtttcat aaaagcaact gtatttttta tatcttcata atctcttcct aaacgatcta
     7501 gtgat
//
LOCUS       DACEDD010000105         7472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEDD010000105 DACEDD010000000
VERSION     DACEDD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.105"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..1751
                     /locus_tag="IWO45_002873"
     CDS             273..1751
                     /locus_tag="IWO45_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0334298.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2028..4088
                     /locus_tag="IWO45_002874"
     CDS             2028..4088
                     /locus_tag="IWO45_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334299.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4171..4740
                     /locus_tag="IWO45_002875"
     CDS             4171..4740
                     /locus_tag="IWO45_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0334300.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4915..5721)
                     /locus_tag="IWO45_002876"
     CDS             complement(4915..5721)
                     /locus_tag="IWO45_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334301.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5859..7373
                     /locus_tag="IWO45_002877"
     CDS             5859..7373
                     /locus_tag="IWO45_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0334302.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2682 a         1227 c         1459 g         2104 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1861 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
     1921 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
     1981 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
     2041 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
     2101 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
     2161 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
     2221 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
     2281 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
     2341 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
     2401 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
     2461 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
     2521 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
     2581 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
     2641 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
     2701 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
     2761 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
     2821 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     2881 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     2941 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     3001 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     3061 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     3121 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     3181 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     3241 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     3301 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     3361 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     3421 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     3481 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     3541 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     3601 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     3661 cccatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     3721 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     3781 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     3841 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     3901 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     3961 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     4021 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     4081 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     4141 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     4201 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     4261 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     4321 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     4381 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     4441 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     4501 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     4561 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     4621 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     4681 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     4741 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     4801 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     4861 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     4921 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     4981 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     5041 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     5101 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     5161 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     5221 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     5281 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     5341 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     5401 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     5461 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     5521 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     5581 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     5641 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     5701 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     5761 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     5821 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     5881 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     5941 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     6001 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     6061 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     6121 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     6181 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     6241 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     6301 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     6361 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     6421 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     6481 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     6541 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     6601 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     6661 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     6721 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     6781 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     6841 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     6901 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     6961 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     7021 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     7081 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     7141 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     7201 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     7261 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     7321 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     7381 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     7441 gaacactgtt tagtgaagag gttgtgaaaa ag
//
LOCUS       DACEDD010000106         7336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEDD010000106 DACEDD010000000
VERSION     DACEDD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.106"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IWO45_002878"
     CDS             complement(<1..142)
                     /locus_tag="IWO45_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0334303.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IWO45_002879"
     CDS             complement(183..437)
                     /locus_tag="IWO45_002879"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0334304.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IWO45_002880"
     CDS             complement(548..802)
                     /locus_tag="IWO45_002880"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0334305.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IWO45_002881"
     CDS             complement(995..1270)
                     /locus_tag="IWO45_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0334306.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IWO45_002882"
     CDS             complement(1242..2195)
                     /locus_tag="IWO45_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0334307.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IWO45_002883"
     CDS             2807..4300
                     /locus_tag="IWO45_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0334308.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IWO45_002884"
     CDS             4435..4731
                     /locus_tag="IWO45_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0334309.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IWO45_002885"
     CDS             complement(4755..5714)
                     /locus_tag="IWO45_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0334310.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="IWO45_002886"
     CDS             5897..6748
                     /locus_tag="IWO45_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334311.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..6981
                     /locus_tag="IWO45_002887"
     CDS             6769..6981
                     /locus_tag="IWO45_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334312.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2415 a         1193 c         1320 g         2408 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacg
//
LOCUS       DACEDD010000107         6889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEDD010000107 DACEDD010000000
VERSION     DACEDD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.107"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1056
                     /locus_tag="IWO45_002888"
     CDS             <1..1056
                     /locus_tag="IWO45_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ0334313.1"
                     /translation="AIDNMDGIMTVCLGRNMLFDLVIQSYSQLETRYDKAFKTIKENC
                     QNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVERAS
                     QLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDI
                     ACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEE
                     ELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKEAIELVKVINGRLADENSADMV
                     IKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMILEQQLANAYELAVFKTKNSILN
                     NKIIKLKNAMTKLAKAI"
     gene            1107..3758
                     /locus_tag="IWO45_002889"
     CDS             1107..3758
                     /locus_tag="IWO45_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334314.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTLIGSTEK
                     LSGIMFAKTLYNDATSINNQDGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNAL
                     KEKNFFSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFS
                     DSFKGGYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIE
                     TGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVF
                     VLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSDS
                     GEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMR
                     DMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAKV
                     ENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELIN
                     QKRQEAKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGLEKLVKE
                     NLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIGVRENLQS
                     SSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQG
                     FKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKP
                     RRRFTYEDNELFRDTLNDPNPLFDNLN"
     gene            3782..4117
                     /locus_tag="IWO45_002890"
     CDS             3782..4117
                     /locus_tag="IWO45_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334315.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            4114..4731
                     /locus_tag="IWO45_002891"
     CDS             4114..4731
                     /locus_tag="IWO45_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334316.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            4748..5215
                     /locus_tag="IWO45_002892"
     CDS             4748..5215
                     /locus_tag="IWO45_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334317.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            5231..6808
                     /locus_tag="IWO45_002893"
                     /pseudo
     CDS             5231..6808
                     /locus_tag="IWO45_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
BASE COUNT         2565 a          899 c         1393 g         2032 t
ORIGIN      
        1 gctatagaca atatggatgg gattatgacg gtatgtctag gaagaaatat gctttttgac
       61 ttagtgattc aatcctatag tcagctagaa acaagatatg ataaagcttt taaaacaatt
      121 aaagaaaatt gtcagaatca tattttaatc atgtcgaacg atgaggaaac gatagaagag
      181 atttcaaaaa aatgtggtca taagacaaca ataaatagaa gtgctagttc taaacattta
      241 gatactgata gctcggtcac tagttcggca gagcaagagc gagtaattac agttgagcgt
      301 gctagtcaat tgattgaagg ggaacaaatt attttaagaa accttcatag acaagataca
      361 aaaagaaata agattagacc atttcctatt tttaatacta aagaaacaaa tatgccgtat
      421 agataccaat ttttagcaga agactttgat actcaaagag atattaatga gattgatatt
      481 gcatgtgaac atatcaatct atctttacaa gataaccaaa ttccatatgc aaggtttatc
      541 aaagatttaa aaacacgttt ggagtattca attattaata atattcctat tagtgaagaa
      601 gactatcagg attatctaaa tttatcagga tcacaacaaa caattaatga agaagagctg
      661 ttaaaactgg taaatacgga agtaaaaagt caagatatgg aaggtcaagt tttatcagaa
      721 caagaaatga aaattaatat ttatctaaag gaaagtcaaa aggaagcaat agaattagta
      781 aaagtaatta atggaaggtt agctgatgaa aattcagcag atatggttat aaaaaccatt
      841 aattcacaaa tccttgatac gcctttcatt caatctttaa tcaatgattc tatagctatt
      901 cataatgttc aagagagtaa ggatcaaatt atgatcctag aacaacaatt agctaatgct
      961 tatgagttag cagtgtttaa aacgaaaaat tcaatactga ataataaaat tatcaagttg
     1021 aaaaatgcta tgactaagct agcaaaagca atttagagag aagggaagaa atattgactg
     1081 aatctattaa ttggagaaaa ctaaatgtgc aatggttaaa gtataaatgg ccaatcatag
     1141 tttccctaat aatcattcta tttatggcct atccgcaacg agtttttgcg ttgccgtgga
     1201 ttacagacaa taaagaggta ttaaaatttt taacaaaata tcaagattat cttcagttta
     1261 ctaaaggtgg ttttggaagc attgtcgttc aaagtttatc gtgggggctg attagaggtg
     1321 catattttgt ttgtaaatca gcagaaaacc ttgttccaca atcgctaagt ttgtttagct
     1381 ttgttgaaag cagtagttta ggtgctgttt atcaatctgt attaaacacg gtggttgtag
     1441 gattaatgat cctttcattg atgtttgttg gttacaagat gattactgga aaaggaacag
     1501 tggatttaaa atcggttgga atgaacgtag tgatgtctat agcattaata cttttaatgc
     1561 cgactctgat tggatcaaca gaaaaacttt caggaataat gtttgcaaag actctttata
     1621 atgacgcaac tagtatcaat aatcaagatg gcggtgtagc atggtcattg attgaggacg
     1681 gtgtaactga tttagtatat atcaataaaa acgatgctta tgatcggttg ggtaaagatg
     1741 gcgtacaaaa aaatgcgtta aaagaaaaga atttcttttc ctacgatttc agtgaagtaa
     1801 tcacagttga tacagctaaa gatttagaag atgataataa aaaagctgaa tatcttcaat
     1861 ctaggttgac accagatggc gaaggaaata tgcaagccgt taagattgat gatggttgga
     1921 tgtcgatgtt ctctgatagt ttcaaaggag ggtactacag gtttactaaa aatacttttg
     1981 cattgacttt aggattactt tcgttagctg tagcctatgt ttttagtatt tttatcatag
     2041 tcaccgctat tttagaactg gcatttaagc gtgtgttagg ggttcttgtt tttgcaacag
     2101 atattgagac aggacagcgt tcaaaagttg ttctatcaga tattctacag tgctatttga
     2161 ctgtaggctt tcaaggattt ggattatcca tgtttgcaat gtttgtttcc tatttaggaa
     2221 caggcggagg gtcttccgtt aatccgttta ttaaggctgt agcttatgta tgtgccgttt
     2281 ttgttttaat caaaggcagt ggaacagtca tgagatactt tggtgttgat attggactca
     2341 aagaaggcta tgggcaactt gcttctgctt ttggaatggg tgcaatgctg tttagaaaag
     2401 gatcaaatgg atttaatcgt gccaaaggaa gcggaaacgg aaatggatca gacagcggag
     2461 aaggagaaga tcgaaaacca gaaaagaatt tcggtgaaac tctatcaaaa aaagctggca
     2521 aaactggaag agctttggga tatgcccatg aacgtggatt atcaggttta gcttcagatg
     2581 gagctacaat ggctagtgaa agagcaacaa aaccatttaa gagtatgcgt gatatggcta
     2641 atgatactaa gaataagttc aaagaaggtt tagatgatgg tacagtgtcc gctattaata
     2701 aaaatagcaa acctatgtta gctaaaaata aagaagatga aactggaaaa tatgctgatt
     2761 ccatgccaac acgtctttcg gatagaaaag atggtgctaa ggttgaaaat gccgatagaa
     2821 ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa tcgaataata
     2881 atcctgtaag tgcaattcag caaaaagttc aacaagatat tgaagaacgt aaaaatgcta
     2941 tgcacggtca agctaagtct gctgaagaat taattaatca gaagcgacaa gaagctaaat
     3001 acacgcctga agcaatgaat cgtgaggaaa tgttaaggaa acgtgtagaa gggcgtacag
     3061 gaactaatac ggacgaaaaa gaagcgttga ccaaagaaag aatccaagaa gctaaaaact
     3121 caaataccgg attagaaaaa ttggtcaaag aaaatctaca aaattctaca agtagtcaag
     3181 gtggtcgatc agtagatgtc cgagaaaata ttcaaggttc attgaatggt catgtcggac
     3241 ggaatgttga tgtaagagag aatttacaaa gttcttcaaa tggtcaaggt actaagacaa
     3301 ttggtgttcg agaaaatctg caaagtagca gtacaagtca accaaaaaca gtagatgttc
     3361 gagaaaatgt acaaaattct acaagtagtc aagggtttaa atcagtggat gttcgagaga
     3421 atgtgcaagg tagcagtgca agtcaaccaa aaacagtaga tgtccgagaa aatgtacaaa
     3481 gttctacaag tagtcaagga ttcaaaacag tagatgttcg ggagaacgtt caaaaagata
     3541 gtggagaaat gaaagaaaaa acacaaaaaa tcaatatcgt tgaagacaga aagtcagcga
     3601 aaaaatttga ttcaaatcat gaaacagtga ttattgatag tgaaatccgt gaaaatgata
     3661 agggatcaaa accaagacga agattcactt atgaagacaa cgaattattc agagatacac
     3721 ttaatgatcc taatccactt ttcgataatt taaattaaat taaaagccgg catgagtgat
     3781 tatgccggct ttttatgagg tgaaaatgat gtatagaaat cccaaaaata taaaacaaga
     3841 gataaagcta tttttcttct atttagtcga tttaggaata gtagccggaa gcgtgatggc
     3901 aagtgtttac tttgcaaaat taatgccaat ttcaccaatg atgaaatttg cactgtactt
     3961 attagcattt gtagtgggtg tttggatgtg cgttagaacg cctaatcacc ctaaagagcg
     4021 aaatatttta atgctgtatt atctctttaa aatggataga aacagatacc acgaagtaga
     4081 gtttaaaaga aatagtaggg ggaaaattga attatgattg ttgctgaaag taatgaaaat
     4141 aaattaagtg gtgtttttga caaattctta gaactagtta ttccaaaaga taaagatatg
     4201 tgtgaacttt taccatataa aaaaatattg aaagacggaa cgttagtgac taatgacaat
     4261 aagtatcaag ccttttttaa agttaaaaca actgacttaa cttctatttc agaagatgga
     4321 tttatgcgta tggtaaacaa actcacttat ttaaatcgag tatatttaga accatataaa
     4381 atttattcat taacttatcc tactgaaaca gtaaatcaag cgatttattg gaagcataga
     4441 atacaggagg caaagagaag tttattgagt aataatgatc ccaatttaga aacgtatctg
     4501 aagtggaatc taaagctagc ttacgataat tatgaaagag ttgatctagt atcgacttta
     4561 ccagagttga tgttcgttat ttgtgtttat ggaaaaacac aaaaagaagt gaaaaatcat
     4621 gccgatgtaa tgaaacaggc cggtggtaga gaattagatt tacaaagaat tactgaaggg
     4681 gatttaatca atgtgataac taaattgatg aatcccaata cttctttata aggcggtgct
     4741 tattattatg ttaaagcact gtagaaaagg attttgtaaa gtaattaaag gtaaaaatgc
     4801 tgctgattta atctttgaat ggaaagaatc taatttaatt aatgtacgtg ggaaaaatgg
     4861 aagcggaaag acagaaagag tgatggctag tattgataat tcaaggccta gatcaagagc
     4921 attagtaata actgatgaaa aaaatcacta taaaggcata cgaacgatag aaaaaaaata
     4981 tttaactaac aatgatttaa aagtagtaga tgatatttta aatatttatc ctgattttaa
     5041 tttagttgtt tttgatgaaa caattcctgt gacagaaata aaaggattct atcaaagctt
     5101 ttccaatcta gcaaataaat atgaggaagt tctgtttgtt tggattacag aagaagatag
     5161 taatagcaag tcagcgttct ttagtgaaaa gaacgctttt ttacattcta tttaaaaagg
     5221 agaattgaac atgattgtaa gtaaaaaact agcaagaaat ctagtaagtg aagggtatga
     5281 cttaagatta gtagaaaagt atcaaccgag cggtggaatt aaatttgatg agcgttatat
     5341 ttctactggt gatggatatg taacttcgat tgctgttatt gagtttgcag aagatgtaaa
     5401 tagtcgatgg ttaacggata taatggcaat tccaaatact attgcaactt tcgatgtatg
     5461 tacggcggat aaagaagact tattacacaa aatcaatacg tctattggag aattagatta
     5521 ccgatcaaca aatgagaata gaacaactga caaaacagac gctttactag ataaatacaa
     5581 tttagaagat tttgcacaag aaattacgaa acgtggtgaa gttgtcaaaa ctgttcatgc
     5641 tcgtattttt gtttatagcg attctttaga agaattagag aaaagaatta gtgagattcg
     5701 taaagatttg gctagtgtca atcacaaggc aattgttccg caatttagaa tgaaggatga
     5761 atggatgtct ttatttcaag atggcatgga acaagaatca aaattatttg ccaagaaagg
     5821 cttaactgtt ccggcaaaaa atattggtgg cggtattcca tttcaccaca agagtttgaa
     5881 agatccacgt gggttttatt taggaactac gtcaactggt ggtgctttta tatttgatcc
     5941 attttattct acgaagcaaa gacggtcgtt taacggcttt attttaggaa agatgggttt
     6001 tggtaagtca actttaatta aacagctaca ggaaggccta caagcgaaaa attgttttat
     6061 tcgaggattt gacaagtcag gtgaatttga aggcatggtg aagaaagcag gcggtgtata
     6121 cgtcgattta ggcggtggtg atatggtagt aaatccattg caaatttatg caacagtcat
     6181 gaaaaaagat caatttggaa atgatgttgt agatgaatca aagagttttc aaaatcatat
     6241 cactaagtgt agtaatcaaa ttcgcttttt agaacctgaa attgatacgg caactgtacg
     6301 tgaatatact ttgatgttac aagactttta tattcattat ggaattttgc caaaagaatg
     6361 gagagaagcg agagacgaca acgaacgaaa tcaattaaat acaaatattt gtaacttgcc
     6421 taatgattca tatccaatct atggagagtt tcttaaattt attgaaaatc aacaattcaa
     6481 aattaaaaat gctactcctg aacgtgtccg ttctattgag ctaattaagc ttatcgttac
     6541 ggatatgtgt acaacttatc cgcaaatttt caacggagta acaacaatgc caaactttga
     6601 tacaacaaaa attgtttact ataacattga ttctattagt aaaatgagtg ccccagtatt
     6661 ccattgccaa ttatttacag cgttaacgac tatttggaac agtgccttgc aaaacggaaa
     6721 gattcaaaaa gaaaacttta aaaaatatgg cagtgattat tataagtatg cacaagattt
     6781 catggttttg atcgatgagt gtcacaatgg tgcgacctgt tctgcagtga aaagctgtgg
     6841 gttgcaagta gtctaaaaac tattgtaata actcttataa cgagaagtc
//
LOCUS       DACEDD010000108         6193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEDD010000108 DACEDD010000000
VERSION     DACEDD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.108"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            164..2761
                     /gene="adhE"
                     /locus_tag="IWO45_002894"
                     /gene_synonym="adhC"
     CDS             164..2761
                     /gene="adhE"
                     /locus_tag="IWO45_002894"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0334318.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2990..4096
                     /locus_tag="IWO45_002895"
     CDS             2990..4096
                     /locus_tag="IWO45_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0334319.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4113..5137
                     /locus_tag="IWO45_002896"
                     /pseudo
     CDS             4113..5137
                     /locus_tag="IWO45_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5143..5622
                     /locus_tag="IWO45_002897"
     CDS             5143..5622
                     /locus_tag="IWO45_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0334320.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5638..>6193
                     /locus_tag="IWO45_002898"
     CDS             5638..>6193
                     /locus_tag="IWO45_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0334321.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         2009 a         1087 c         1379 g         1718 t
ORIGIN      
        1 ctttatatat ttttctatac aaacacctgt aaataaagca attttatatt taaaaataac
       61 tgtgaaaaaa atcacaaaaa agtgtttaca tagaaaaata aatattgtat gatatacatg
      121 tgaattattt aacaaataac catgacccat taggaggata atgatggcta aagtaataga
      181 aaaagaaaaa acgaagacaa ttgacgtaca agcaatgatt gatgatttgg cagaaaaagc
      241 aaatgcagca ttgaaagaaa tggaaagctt tgatcaagaa aaagtagacc atatcgtgca
      301 tgaaatggca atggcagctt tggatcaaca tatgccatta gcgaagatgg ctgtagagga
      361 aacaggccgc ggtgtttacg aagataaagc aatcaaaaat atgtatgcgt ccgaatatat
      421 ttggcataat atcaaacatg ataaaacagt tggtgtgatc aatgaagatg tccaaaaagg
      481 gttgattgaa attgctgaac cagtcggtgt ggtttgcgga gtaactccaa caacgaatcc
      541 gacttccaca acgattttca aatcaatgat cgcgttgaaa acaagaaatc caatcgtttt
      601 tgctttccat ccaagtgccc aaaaatcttc tgcagaagca gcacgagtgg tacgtgatgc
      661 tgctatcgct gccggagctc ctgaaaattg tatccaatgg atcgaacatc cttcgatcga
      721 agcgacttct atgctaatga accatccagg tatcgcgatc gtcttagcga caggtggagc
      781 tggaatggtc aagtctgctt actctacagg aaaaccagct ttaggtgtag gtccaggtaa
      841 cgtaccagct tatattgaaa aaacagcaaa aattaaacgt gcagtgaacg atttgatcgt
      901 ttcgaaaaca tttgataatg gaatgatttg tgcttctgaa caagctgtga tcgtggataa
      961 agaaatctat gcagcagtaa aagcagaatt ccaagcacac caagtttata tcgtaaaacc
     1021 agatgaattg caaaaattag aagatgcagt gatgaacgaa ggaaaatatg cggtcaatcc
     1081 atcaatcgtt ggacattctg cgatggaaat cgcaaaattg gcaggaatca gtgtaccaaa
     1141 aggaacgaaa atgctgattg ctgaattgga aggcgtaggc ccagattatc cgctatcaag
     1201 agaaaaactt tctccagttc ttgccatgat caaagctaac aacacggacc atgcgtttga
     1261 tttgtgtgaa ggtatgttga atcttggagg attaggacat acagcagtta tccattcaga
     1321 aaacgaagag ttgcatgtca aattcggtct tcgaatgaaa gcttgtcgta ttttagtgaa
     1381 tacaccatct gcagaaggcg gcatcggtga tatctataac gaaatgattc cgtcattgac
     1441 tttgggatgc ggttcttatg gtaagaactc tgtttctcgt aacgtctctg ctgtaaactt
     1501 aatcaatgtc aaaactgtag cgaaacggag aaataacatg caatggttca aattaccgcc
     1561 aaaaatcttc tttgaaaaga actctctatt gtacttagag aaaatggaaa acgttgaacg
     1621 tgtcatgatc gtttgtgacc ctggtatggt ccaattcggt tactgcgata cagttcgtga
     1681 agtattggca cgacgtaaaa atgatgtgaa gatcgaagtc ttctcagatg ttgaaccgaa
     1741 cccatcgaca aataccgtat acgcaggaac gaaactgatg gcagacttca agccagatac
     1801 agtaatcgct cttggaggcg gttctgcaat ggacgcagct aagggaatgt ggatgttcta
     1861 tgaacaccca gacacatctt tctttggtgc aaaacaaaaa ttcttggata tccgtaaacg
     1921 gacatataag atcgacaaac cagaaaaaac acaattcgta tgtatcccga caacatcagg
     1981 gactggttct gaagtaacgc catttgcggt catcactgac agtgaaacac atgtgaaata
     2041 tccgttagca gattatgcat tgacgccaga tgtcgcaatc gtcgatcctc aattcgtcat
     2101 gtcagttcca gcatctgtca cagcagatac cgggatggat gtgttaacac atgcaatcga
     2161 atcctatgtt tcagtcatgg cttcagatta tacacgtgga ttgagcctac aagcaatcaa
     2221 gttagtcttt gactatttag aaaaatcagt aaaaacacct gatatggaat cacgtgaaaa
     2281 aatgcacaac gcttctacaa tggctggtat ggcgtttgct aacgcattcc taggtatttg
     2341 tcactcagtc gcacataaaa tcggcgggga atacgggatc cctcatggac ggacaaatgc
     2401 gattctttta ccgcatatta tccgctataa tgcaaaagat ccacaaaaac atgcgatgtt
     2461 ccctaaatat gactatttcc gtgcagatac agattacgca gatatcgcaa aattcttagg
     2521 tctgaaaggc gaaacaacag aggaattagt cgaagcttta gcaacggctg tttatgaatt
     2581 aggaaaatct gtcggcatca acatgagtct aaaagctcaa ggagtcacac aagaaacact
     2641 agatacaacc gtagaccgta tggctgaatt agcttacgaa gaccaatgta cgacagctaa
     2701 tccgaaagaa ccattgatct ctgaattgaa acagatcatc attgatgctt acaacggata
     2761 agcaaaagaa agcgtagaaa aaagataaga aaatagatga aaaagaactt ggaaaacgtt
     2821 gtttctccaa gttctttttt taatttccaa gtaactgaaa gttacttttg ttatttaaca
     2881 tttaggtcaa ttttagacag tttttgttca aaaagcgata tttatagtgg acggtattac
     2941 tgctatatta aggatataaa atgaaagcgt ttaaaaaggt ggcgtaaaga tgattattaa
     3001 agaagagatt tttgtaccta aagaattagt gaaagaaaaa attgacctgc tcgttgaaaa
     3061 cctgacaacg atcaaagacg ataatggtga atttttgttg gactttgatg gactgaaagt
     3121 agatgacaaa agctggacag tgtggaattg gccacaaggt gtcggtctat atggtattta
     3181 taaaaattac cagatgacaa aaaatcctcg cgcttatcaa gtggtcaatg aatggtttga
     3241 ggatcgtatg gaagaaggcg ctccgcccaa aaacgtcaat acaatggcac cgctattgac
     3301 tatggcttac ttgtatgaag acacgaaaga ttcacgctat ttgccttatt tagaacaatg
     3361 ggcagaatgg gtcatgaatg acatgcctcg gacaaatgaa gacgggttac agcatgccac
     3421 atatggaccg gaaaataaga atcagttgtg ggacgataca ttgatgatga cagtgcttcc
     3481 tttggcaaaa atcgggaaac tgttgaatcg cccagaatat ttagaagaag ctcgttacca
     3541 gtttatgatc catatcaaat acttgatgga taaacgaaca ggattgtggt atcacggctg
     3601 gacgtttgaa ggaaatcata actatgccga agcattttgg gctcgtggaa actgctggtt
     3661 gacgattgct attccagaga tcattgaaat cttagaatta tcgaaagaag atgcattgcg
     3721 caaattattg atcgaaacac ttgaagcaca agtgcgtgca ttaaaaactt atcagtctga
     3781 aagcggttta tggcatacgt tgttggatga tccttcttct tacgtagaat cttctgcaac
     3841 tgcaggattt gcttacggta ttttgaaagc ggtccataaa cgttatttac cacaagaata
     3901 taaagaagtc gcttatcgtg ctatccaagg attgcttgaa caaatcgatg aaaaaggaga
     3961 agttcaaaac gtttcaatcg ggacaggaat gggggacagt ttagattttt atcggaatat
     4021 cggaatcaca gcaatgcctt atggacagtc gttaacggtg ctttgtttga cagaattgct
     4081 cgtctcttat tgttaaaaga aaggaaaccc atatgaaaaa aacatcactg ctgtttgcag
     4141 caagttcgct tagcctaggt ctgctaggtc taagcggctg cggaaataat tctagtgtgg
     4201 caactgtcgc agatgcagac gtaaatgaga tagttacctt gcgctttgct tatgcaagca
     4261 acagccagta attgatgcaa tgaatgaatt tgggcgttta gtaaaagaaa aaacagatgg
     4321 ttccgtacag atccagtatt tcccagatgg acaactgggt ggagaacgtg aactgatcga
     4381 attgacacag tcaggagcag tagatttcac aaaagtaagt gcctcagcgt tggaaagttt
     4441 ctctaaagat tattcgattt tttccgttcc ttatctattc aatgatgaag aacatttctt
     4501 taaggtgatg gataatgaag aaataatgga gccaatctac caatcgacca aaaatttagg
     4561 atttaccgga ttgacctatt atgattctgg tcagcgcagc ttttatatgg tagacggtcc
     4621 gatccatacg ccggaagatt tgaagggaaa gaagatccgt gtgatgcaaa gcgaaaccgc
     4681 tatcaaaatg gtagagcttt taggcggttc gcccgtacct atgggaagtg atgaagtgta
     4741 cacgtctctt caatccaatt tgatcaacgg atcagaaaac aatgaatttg tcttgtatac
     4801 agctggacat ggcggggtag ctaaatacta ttcctatgat gagcatacac gtgtgccaga
     4861 tatcatcatc atgaatgatg ccatcaaaga acagctaact gatgaacagg aaaaagccat
     4921 tgaagaagca gcgaaagaat caacggtttt tgaaattgaa gcttttgcaa aagcaattga
     4981 agaagaaaaa gctttagcac aagaagaaca tggagtccaa ttcaatgatg tagataatac
     5041 accgtttcga gaggcagttg ccccacttca tgatatattc aaaaacgatc ctacctacaa
     5101 agacctttat caaaagatcc aagagcaagg agcgtagctg taatgaaaat gattttaaac
     5161 aaggtattag agatcctggg agctgttttg cttgttggga tggtcgctgt tgttttatgg
     5221 caagtatttt ccagaacggt tttaggaaat ccaaatacgg tcacggaaga gcttgttcga
     5281 ttcggtcttg tctggtttgc catgttgtct tccgcgtatg ttgtgggaca aaaagcacgt
     5341 ttagccgtaa cggttttaag tgaaaaatta actggcaaaa aaagtaaaat tttagaagtt
     5401 gttgttcaaa gtttatttct ggtatttgct gcgatgatca tggtgtatgg cggatggaat
     5461 gctgtcacat tgacaatggg gcagatctcg ccttctttaa gtattcctat gggatatgtt
     5521 tatctgtcag tacctgtatc gggggtactc atcattatct atagcctgat caatctaatc
     5581 gatacattaa gcaacaagcc agcaattcaa gaaaaaatat aaaaatggag gaataaaatg
     5641 gcaatagaag ccggattagt gttatttttt gtatttgttg tgttgcttgt attagggatg
     5701 ccgattgcta taagtattgc attctcatcg atgatgactt tattgttagt tataccgttt
     5761 gatgtttctg cttttagttc agctcaaaaa atggtgggaa gtatcaatag tttttcgctg
     5821 atcgctattc cgtttttcgt tctctcgggc attatcatga ataatggagg gatagctaag
     5881 aaactagtgg attttgcgat gttgttcgtc gggcgcgtgc cgggggcttt ggctcatacg
     5941 aatgtgttag ggaatgcttt atttggttcc atctctagct cggccattgc cgcttctacc
     6001 gcgattggtg gagtgttgat ccctcagcaa gtggaagaag gatatgaccg gaagtttgcg
     6061 acagcggtca atatcgcgtc agctccgaca ggtatggtca ttcctccaag tactgctttt
     6121 atcatgtatt cacttgttgc tggtggtgct tcgatctctg ctttgtttat gggaggttat
     6181 ctcgtaggcg ctt
//
LOCUS       DACEDD010000109         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEDD010000109 DACEDD010000000
VERSION     DACEDD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.109"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWO45_002899"
     CDS             725..811
                     /locus_tag="IWO45_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0334322.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWO45_002900"
     CDS             complement(932..1621)
                     /locus_tag="IWO45_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334323.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWO45_002901"
     CDS             complement(1628..1906)
                     /locus_tag="IWO45_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334324.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWO45_002902"
     CDS             complement(1866..2780)
                     /locus_tag="IWO45_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0334325.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWO45_002903"
     CDS             complement(2762..3142)
                     /locus_tag="IWO45_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0334326.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWO45_002904"
     CDS             complement(3592..3879)
                     /locus_tag="IWO45_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0334327.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWO45_002905"
     CDS             complement(3899..4123)
                     /locus_tag="IWO45_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0334328.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWO45_002906"
     CDS             complement(4763..5296)
                     /locus_tag="IWO45_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0334329.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWO45_002907"
     CDS             complement(5317..6159)
                     /locus_tag="IWO45_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0334330.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACEDD010000110         6131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEDD010000110 DACEDD010000000
VERSION     DACEDD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.110"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1668)
                     /locus_tag="IWO45_002908"
     CDS             complement(<1..1668)
                     /locus_tag="IWO45_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0334331.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAV"
     gene            complement(1831..2424)
                     /locus_tag="IWO45_002909"
     CDS             complement(1831..2424)
                     /locus_tag="IWO45_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334332.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2425..2757)
                     /locus_tag="IWO45_002910"
     CDS             complement(2425..2757)
                     /locus_tag="IWO45_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334333.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2809..5340)
                     /locus_tag="IWO45_002911"
     CDS             complement(2809..5340)
                     /locus_tag="IWO45_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334334.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            complement(5337..>6131)
                     /locus_tag="IWO45_002912"
     CDS             complement(5337..>6131)
                     /locus_tag="IWO45_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ0334335.1"
                     /translation="PMDNMEGIMTVTAGRNMLWDLFIQSYQQLYAKYGEQNAAIIKEN
                     CQTHIYIMSTDDNTIEEFSKKVGNKTVEQENSTINSLGMNTHINRNVDSDRILTPERI
                     STFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQYIEDFNPSWDINDIDI
                     HCTHADLDLKALQIDYDYFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSLENETAK
                     KLLIQFYEEQAADRLQEYVEKLIKNKEISEMIGKRLLLESEKVVKE"
BASE COUNT         1662 a         1272 c          985 g         2212 t
ORIGIN      
        1 cactgcagaa caggtcgcac cattatggca ctcatcaatc atcgccaaga agcgtttgaa
       61 gtcttgttct tccatttgtt tttctttaat taaacgtcgt tgtcttcttc cttgaatcaa
      121 tgcctgattc caaatcaacg tcatagccat gtacatttga cattgttgta cccctcgatc
      181 taagcctgta atagagtcaa tgtcaaaaaa gaccaactgt tgttggtcaa ggtgtcgaat
      241 cgtactatga ccatcaaaaa tcatgccata cgtctcacat aaggattcta cgatggattc
      301 catttcttct tttcgattct ttttctcttg ggaagcacct ggtaattcca gttgtcctaa
      361 aaagtctaaa aattctttga acgtcggata agcttctgga tctttccctg tgacttgcgt
      421 atcagtattt gaacgcgaat ttgaggaagt aaagccgcgt ggtactgttc catactcttc
      481 ataaaaacga tttaagtagg tcttaaattc tgctttcatc gttcctttta gctgaggact
      541 cactaactgt acctgataga ccactttgtc taagtgtgtt tgataggacg ctgcttcatt
      601 gatcttcaaa tctttgactg atccatcgtc atataaactg gatgcttcat catacgtccg
      661 tgtggcaaat atctctaaag gattgatcat tccgtcagat ccgtctaaac gcaccataat
      721 accgttatat tcctttaaga aaggaataaa gtcttttccc ttttcaaagc cccaaataaa
      781 gtctcctgca gctaaatggg cttccgcgat cattttaaga acggtcgatt taccagctcc
      841 catcattcct agaagcaagg tattagtaga caaccgtaca ctatttttat acgtcgcatt
      901 ccagacaaat gggccgcctg tcatggtttc cccatattgt gtccctcctg gatcttttag
      961 cgtttggtgg tggaacggga aacctccgcc aatgtttaac gatggtagtg attgcccttt
     1021 gatatttccg aaccattcag cttgttcatc gaacatggta aacatcgatt gccaatctcg
     1081 tttggtttta tgcagataag tcaccgcacg ataatccatc cctttgagat tttcgatcag
     1141 ttcctgcgct cgttcatcta cttcttgaat cgtgtttccg taaacatgta ctctcacttt
     1201 gacttgctta gaaatttccc cattagaagt caattttcta gcatattccg ctagttccag
     1261 gtattcttcc gcgcttttat ttcggtcagt cgcttttcgt tcattatctg atcgatcatt
     1321 taattcatct aaggcacgat tgatttgatc aatcactttt tgcttttcat ctgtgctaac
     1381 atcgagtgtg gccactgtat gaggattgtt catgagattg gccaaccaaa aaagcgtagg
     1441 attttttgtg tagtgataaa ctgtcacaca tcgtgaataa ccattaccat acgtcacata
     1501 ttcttcatca aaatttaacc caccttgtgg catcacttgt tttaaaaaat ctaaatcata
     1561 ccctttggat tttacaattt ctatttcccg atttcctagt tgctttaatg gatcaagtac
     1621 tttttgatca ttttgtggtg aaaaagctgt tttaattgct tctaacatgt acagtcacct
     1681 cttcttgttt gattctttac ttggttaaaa gattgccctt ttcccaactt acccaggctt
     1741 aaagtgtgag gaaattttct agtggtctct tccttcggaa catctttttc taaccatatc
     1801 aacggatatt tcattctctc ttctccattc ctactcatca ttcatattgt tcattcgata
     1861 cagaatcttg atcaattctt ttttgtagag tggttttaac cctaaaatgc cactagatcg
     1921 cattaactga cggtttctgg tttgtaaatc cttgattgtc tttccttcaa tgacgaaaaa
     1981 gaagcttaag tcaggacgtt cttgttctgc tcgtttcaat tcctggattt cttcgactaa
     2041 tttcttttca tattttttta atcgttgtag atttcttcct aaggactgtt ccatacgatt
     2101 tttatctaat aattgccgct tttgcacgat ttgttccgtt agatccgtct ctgtggtcaa
     2161 actaactaac ttaattgttg gcacatacgt acgcatgacg gtggtcaact gattcattcg
     2221 ttctagtttc tcttgttcat ttaatccttc cacgtctgta gaaactacac gataataacg
     2281 ttgaaaatga tcttctctag taattaagac tccctcttct gtacaggatt tatatggaaa
     2341 taagtccaag atagagcgct cttctggttt tccaaactgc aaaatggatt tcctttcaac
     2401 tgaattttgt cctttgactc gcatttattc atcctcctta tacgtaccat gataatttcg
     2461 gttaggatct aacgcatgat aatggttctt gtccgcaaaa aaaatagact cgataatcgc
     2521 aaaaatttgt cgttgacctg ggttattata gggtcgccaa acaatcacta acgctatcgc
     2581 aatccataag ccataattca agagtgcaaa aaagagtggt aagtggacaa gattattgac
     2641 cattttcgcc caaaaaaagc agccaccaag cgttaataga tctaaaaaga aaaaagcgaa
     2701 aaacttccat tctccactaa atcttggagg gtttttcatg ttactatatt tatccataaa
     2761 tgatttctcc ttttcataca tagaaaaaag gcctaacttg taggcctttt atggtttgat
     2821 atcctgtttg atatccctca atagttgttc tttcaatgca ttgttcctgt tttttgtttc
     2881 tcttgtcaca tttatttttg ttggtgcagt aaatgccaca tttgctggct tgtaacctgt
     2941 atgaacgttt tgaataggtt ttggtgcagc ttctcttgtc acaacatttt gaacggacgc
     3001 attccctgtc tcaccacttt cagataaatt gtaagaaaca gttgtcagat ttccattttc
     3061 aggcaaacga cttggtttca tttgaacagt acctgcaggt gattgatgaa caaaactttg
     3121 atcattatcc ttgaattgat tcaaacgact atctttcatt ggtaaatttt gtgccttagg
     3181 taaaccaatt ttcgttgatt tttcagttcc aacttcgatt ccattatcat ttggcagaac
     3241 gatggaatcc tcatccgttt gtacttcttg atttcgtttt ggtaaatctg aaatgcgttt
     3301 ttcattttcg gagttaacag atctataccg atattgttct ggatcagttt ttggtaactg
     3361 tccctcattt tctgctttgt cttcttgatt tacttttgat aaatctgaaa ttcgcttctc
     3421 attttcaagg tcactgttag gtttatagcg atattgttct ggatcagttt ttggtaactg
     3481 tccatttttt ccttcttctg aatttctttc ttctatggaa tttttctctt caatcgaatt
     3541 ttcattagag tgcaacgctt cttggtgatc tttgactgaa ggaatcacta taccttcttg
     3601 attttctttt tcggttgttg tattagcttt cactgctgca ttgacttgtc cttcaccata
     3661 cgcatctttt agtccttctt tgacttctga agctttttct ttttgttttt ctagtgcttc
     3721 ggatggattc ttcacagctt cagccaattc tttagctctc gtgccgtcta atttatcttc
     3781 taaggtgtct ttggcttttt ctgtaagatc ttcagctgtt ccttttaacc cacgttcatt
     3841 gacgtaacca agaccttctg cggctttaga aacaccattt ttcgctttcc cgaacatttc
     3901 ggaaactgtt ttcttactat taggtacagt tgtagcttta tcgtctgatt gttcttcccc
     3961 gttatcaggg gtaacatctt ttcgatggcc aaatccattt ttacgaatat catttaaatg
     4021 gcttaaacca ctcttaccag cacttgcaag gtttttagct ccgctaccca catttttcgc
     4081 aatatttcct aaggcaaaca tgctaagaag agaattactg ccgtttttca ggcttgtatc
     4141 aacgccgaaa attttcgtaa ctgattgaga gcctttgaat agcgcaatgg ttagtacaat
     4201 caaagcaacg acatacaaga atccattgat ctttcctgcc gcatgcaaat caccgatacc
     4261 tgaaagcatt aaggtataga ttcgtaattc taaaatcgta aacgccaata acaagaagga
     4321 ttgagaaata tcttctatcg cacttttcat ccgttgacct gtctcaaagt ccgttgacgc
     4381 ggcaatcact aaatagaatt ttttaaatac cagctcaata atgcaagtaa cgatcgtaaa
     4441 taggataaat aaataggcta ctgccaaaga agattcacca gctaaaataa tacctggttt
     4501 actactgtat cgacgatacc ctgaagtata gacatatttt gctaagcctt cgtcactaat
     4561 ttgaacagga atttctttcc cattatcatc tagttccaat cggtattgta atgcctttaa
     4621 ccgatccttt tgtatatctg ataacccact tttatcatta cctgccatac ctttagcttg
     4681 ttcaggagta ataatcccat tgatattcgc tgttttaaaa tttttcgctg ttaaagcatt
     4741 tcttttatct gttggtaggg attctacttt attcgggtca gttgctagta acgtactgac
     4801 atcgatcaca ttattttgaa taatttgaaa agcaggtgat cccgttttat ctgctttatt
     4861 tgctgcagtg acgtctccgt aaaagctttt actgatattt aacccttcat cgattaaaga
     4921 actaccacca aaaatcaaga tcatggccat aaataaatta accaagatat ttttgacttt
     4981 tggtgggtgt ttactcataa aaaatttcaa gcctaaataa attaatgtga cgacacaaaa
     5041 aagagccaat accttactaa tcaaactttg aaatagtgca tttaccccag cggcgttcaa
     5101 taagtctttt aaatcaaagc tttgataaat ggttttttct agtaaagtat tgagattata
     5161 taatccttta atcaatcccc acagaaaagt gttccaaatc caactaaata catcttgata
     5221 ctgcagataa ttcccataat cttgtaagac tttcgttacg gttttggggt ttgcatcccc
     5281 ttctccgaat aagcctgacc accagtcggc tatgccagaa aaaattcctc caatgatcat
     5341 tctttcacca ccttttcaga ttccaataac aaccgtttcc caatcatttc agaaatttct
     5401 ttatttttga tcaacttttc aacatattct tgaagtcgat ctgctgcttg ttcttcgtaa
     5461 aattgaatta acaatttttt agccgtctca ttctcaagag attgaacgat tttataaaac
     5521 atattttctt cgtttttttc ttctaattga tcatgcagtc catgatagag atttctagca
     5581 tattcatctg gtaggaaata atcataatca atttgtaaag ccttaagatc caagtccgca
     5641 tgtgtacaat gtatgtctat atcattgata tcccacgatg gattgaaatc ttcaatatat
     5701 tgataagcat aaggaagatt cgtttcccca tgattaaaaa taggatacgg ccgtatttta
     5761 tgacctttca aatcttgtcg ttttaaagga gcgagtacaa tcgtttctcc ttctaaaaaa
     5821 gtagaaattc tttcaggagt taagatacga tctgaatcaa cattgcgatt gatatgagta
     5881 ttcataccta atgaattgat ggtactgttc tcttgttcaa ccgtcttatt ccccactttt
     5941 ttagaaaatt cttcgatggt attatcatct gtcgacataa tataaatatg tgtctgacaa
     6001 ttttctttga taatcgctgc gttttgttct ccatacttcg cataaagttg ctgataagat
     6061 tgaataaaca gatcccacaa catgtttcgg ccagctgtga ccgtcatgat tccttccata
     6121 ttatccattg g
//
LOCUS       DACEDD010000111         6117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEDD010000111 DACEDD010000000
VERSION     DACEDD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.111"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWO45_002913"
     CDS             <1..177
                     /locus_tag="IWO45_002913"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334336.1"
                     /translation="YEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTT
                     FIADLYKENGVELK"
     gene            174..1478
                     /locus_tag="IWO45_002914"
     CDS             174..1478
                     /locus_tag="IWO45_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0334337.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1574..2443
                     /locus_tag="IWO45_002915"
     CDS             1574..2443
                     /locus_tag="IWO45_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0334338.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2555..2875)
                     /locus_tag="IWO45_002916"
     CDS             complement(2555..2875)
                     /locus_tag="IWO45_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0334339.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3045..4004
                     /locus_tag="IWO45_002917"
     CDS             3045..4004
                     /locus_tag="IWO45_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0334340.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            4094..4489
                     /gene="dhaM"
                     /locus_tag="IWO45_002918"
     CDS             4094..4489
                     /gene="dhaM"
                     /locus_tag="IWO45_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0334341.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            4491..5474
                     /gene="dhaK"
                     /locus_tag="IWO45_002919"
     CDS             4491..5474
                     /gene="dhaK"
                     /locus_tag="IWO45_002919"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0334342.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            5502..6107
                     /gene="dhaL"
                     /locus_tag="IWO45_002920"
     CDS             5502..6107
                     /gene="dhaL"
                     /locus_tag="IWO45_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0334343.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         2055 a         1103 c         1325 g         1634 t
ORIGIN      
        1 tatgaagatg aaaaattgaa aaaaagacaa gaattacttg aagaagtgta tacggaacgt
       61 tttggaacaa aagaagagag agaaacggtc cgtttttatt ctgtctccga agagaaaaat
      121 ctagatacga cattcattgc agatttgtat aaagaaaatg gggtggaact gaaatgatct
      181 caatcaccca tacaggtaat ttattcttag atacttgttt gtctattatg tatttttttc
      241 tcgtttctta tccgatttta ggcggatttg tatggtttat tggtgtctgg tgttatgtgt
      301 ttttatacaa acataagcaa aaagaatggg tagatgttcc cttgagtgtc gaaccgttca
      361 tcacgatcat ggtgccagcc cataatgaag aaattgtgat cgaagataca atcgagtatt
      421 tgatgacgaa attgaattat cacaactatg aggtattagt gacagacgat ggttcaacgg
      481 accaaacacc agaaatatta gctagactga tgaaaaaata cgctaatctt cgtgtggttc
      541 ggatcgaaaa aaataaagga aaagcccacg cgtttaatat cggacttgcc tttgcaaaag
      601 ggaaattgat tttaagcaat gatgcagaca cggtaccgga gccagatgca ctgattaggt
      661 atgtgaatta tttcattcgc cctggtgcaa gacatattgc tgcggtcact gcaaatatgg
      721 atgtccaaaa tcgtaccaaa ttgattgcaa aatctcaaac ggttgaattc tcaagtatcg
      781 tgggcatcat taaacgaaca cagtcagctg ttttcggtgg tctttatgcg tacagtggag
      841 caaatacgat gtatcgaaaa gaagccttga tcgatgtggg cggattcaga caagatcgag
      901 cgacagaaga catcagtatt gcttgggatc atcagttaaa tgactggtta tctgtttttg
      961 ctcctgagat tatctttttc atggaagtac ctgtgacgtt gaagatgcta tatcgacaca
     1021 gaaaacgttg ggcaaaaggt ggaacagaag tctggctgac gaatttcaaa aaagtgatgc
     1081 tccatccttt taaacatatt ggaagaacga tcatcttcat tgatcaaaca ttgagtattg
     1141 tctggtcaat attcttttgt atttccgtag ttctttttgc tggattaatc gggcattatg
     1201 tatatcaagg aaactacgaa cagatttata ttacttttac attcagtttt gtgttcatct
     1261 gctttgaaat ggtcgcagga tttttccagt tgctagcttc gctgattgta gatgatcgac
     1321 gaagaaaatt gaaatacctt cttttcgcac cactatatat gcttttgttc tggatagtga
     1381 acgcgataac gatcgtgacg actttcattc cagcagtcaa aacgatttta ggttatggaa
     1441 gcgggacatg gaaaagcccg gaacgtacaa aaaaataaaa gatgcaccgt tttttagaca
     1501 aacagcctgt tgtgtttatg gttcttccaa acatttttgc ttataataag aaattagtta
     1561 aaaagtagta aggatgggaa aagtgatgaa aaaaagaatg aagccgttaa tctggccaat
     1621 tgctgtttcc agtattttct tattgggaca gacagctttt gcggaaactt ctgaacctgc
     1681 tcaaacagaa ccttcaacaa ccataagctc ttctggtgct gaaaacggtg cctcggaaac
     1741 agaaatgact tccaatgtac cgacagataa ttcaggagaa acgatttcat cttcagaaga
     1801 tacatcaata aatagttcta cctcttctga ccaaatcgat catttgcaag acttggaaaa
     1861 taaaagaaaa gaactcgaag aagcattaga agctgcaaaa aacaaattga aagaagacca
     1921 agcagcaatc gatcaagatt tagctgatct taaaaataaa gtgggaaatg accgtgcaca
     1981 acttgagaaa aagcttgccg aagcgaaagc aaaagcggaa gctgtccaaa cagatcttga
     2041 cgggaagatc caacaagcaa aagaattcaa tcagcaatta aatcaggttg ctcaaacagc
     2101 ttctggaaag ctaagtcaaa ctcaagcgga tatcacagcg gccagtcaag caatccacgg
     2161 aaagatcagc gaagcacaaa cagttgctaa tgattttgaa gcaaaaaaag ctgacttagc
     2221 agctagtttc caacgaataa aagatatttt gcattcggca ggagaaaaat cagtgacctc
     2281 tggaagcacg acagaaacag ttcagcaaac gacagagcca tccaaatcaa caagtcagac
     2341 tttgccaaaa acaaatgaaa aaacagcttc tgtcagttat tccttagcag gggcgggtgt
     2401 gttagtcgca gcagcttacg gaatcatccg caaaaagaaa taaaaaagag aggatgggaa
     2461 ggtagagtac ctcgaagtta gactgaaaaa tctaattctt gagtgccact acatgtccca
     2521 tctctcttta gttattaaaa cagatgtccc ctacttatct cgtggagaca gctgttccac
     2581 tacatgtgac catcagcatg ccattgtcgc tgcctaatac ttcatagtcc atttttacgc
     2641 caatgatcgc atcagctcct aatgcttttg cacgttcggc catttcggca agtgcttctt
     2701 ctcttgcacc cattagttcg ttttcatagc tttttgagcg gccgccaaag atatttcgca
     2761 accccgcctc caagtcttta aatgcatgga tcccagtaat gacctcacca aaaacgatcc
     2821 ctttataagc tactacggct tttccctcta cactgttcgt tgttgttgta atcattcgat
     2881 cactcctctc ttttaattaa agtataacaa acttcaagaa gcactacaca tattttccca
     2941 aaattagaaa tcgccgattg taaaattaag ctagacaact aaaaaagtca tttgtgctac
     3001 actcaaaata gttccgtcga aagaattata aggagtgagt acaaatgacc tatacccatc
     3061 ttacaccaac tgaactcgta atgatagaag cttatttcaa tcaatcacac tctgtttcaa
     3121 aagtagccca tctggtacaa cgttctcgac aaaccattta taatgtctat cgttttctta
     3181 aatctggtgg aagcgcaata agctactatg aacaatataa gaaaaacaaa cgtaactgcg
     3241 gaagacgtcc gattgtgtta ccagatgggc aacaagaata cattcaaaaa aaggtggctc
     3301 aagggtggac tccagatgtc cttattggtc gagcagagtt tcctattgct tgttcggtac
     3361 gtacactata tcggatgttt aaagataaaa cctttgaccc tcacgattta ccaatgaaag
     3421 ggaaaagaaa accaaacgga cataaagaaa aaagaggaaa acaagctttt cgccgttcga
     3481 ttcaggatag aacaactgat tatatacagt tcaatcatga gtttggtcac ctagaaggtg
     3541 acaccattgt cggtggaaaa cataaaagtg cggtgatcac gttagttgag cgattatcta
     3601 aagtgatcat caccttgaaa cccgctggta ggcaagctat agatattgag aatagactga
     3661 atcaatggtt tcaatctgta ccgaaacact tgtttaaatc catcacattt gattgtggaa
     3721 aagagttctc gaattggaaa aagattagta ataccaatga tatttcgatt tatttcgctg
     3781 atccaggaac accttctcaa cgaggactaa atgaacattc gaatggctta ttacgtaaag
     3841 atggcttata taaagctatg gattttaata aggtatccga aacctttatc caatcggtgg
     3901 catcaaaacg aaatcatatt cctagaaaat cattacacta tcgcacacca ttggaagtat
     3961 ttttgagtta cgtaggtaaa gatgatttgt ctagcttaat ttgacaaacg aaaaatttat
     4021 tatatacttg aattgttcat taatgaaagc gaaaccaatc gttagcaact atgtaaaaga
     4081 aaggaagatt cttgtgaaaa aaagtatttt gcttatttct catagtcaaa aagtaacaga
     4141 tggtattaaa gaaatgattg agcagatgca aaaatccgaa gaggtgcaaa ttttttctct
     4201 cggcggaata aatggcgaga tcggatcaga cccgatgaaa atcgtagaag cagtcaatga
     4261 atcaagcgat gcagctagtt attttgtttt tgcggatatc ggtagcgcgg tattgaactc
     4321 ggaactggca aaagacatgt tggaagaaga gcagcagcaa cgttatcatc tggtggatgc
     4381 gcctttagtg gaaggagctt ttgcagcagc catcactgcc ggtataacag atgatactca
     4441 gcaaatctta acagaagcac aaaatgccgg aaagaaagga tgggaataaa ttgaaaaaaa
     4501 tcatgaatga tccaagcaat attgtcgaag aaatgctgga aggattagta aaaagctatc
     4561 cagaacttgt tcatcgtgta gaatcttccc gtgttgtagc caagaatcag aaagcggaac
     4621 aagttggttt ggtttctggt ggaggaagcg gacatgaacc atcccatgcc gggtttgtag
     4681 gggaagggat gttaagtgca gcggtgcttg gtgatgtgtt tacttctcca actccagatc
     4741 agattcagac ggctattaag gaagccgata gtggaaaagg agttttgctg atcgttaaga
     4801 attatacagg ggatgcattg aacttcgata tggcaaagga attagctgct atggacgata
     4861 ttgaagtaga aagtgtgatc gtagatgatg atatcgccgt tgaaaatagt acgtatactg
     4921 ctggaaaacg cggagttgca ggtactgtcc ttgtacacaa aatcgtgggc gatgcagcaa
     4981 gaaacggtgc ttccctagct gagttgaaag aactgggaga aaaggtcgta caggcaacta
     5041 agacgatcgg tctagcgctt cgagcagcaa ctgtaccaga agtagggaaa ccaggattcg
     5101 aactaggaga agatgagatc gagtatggtg ttggtatcca cggtgagcca ggctatcgtc
     5161 gagaaaagat gcagccttct aaagtactgg caaaggaact tgttactaag attttagatg
     5221 attactcgaa tttaccaaaa gaagcaggtg tactagtcaa cggaatgggc gggacgcctt
     5281 tgatggaaca gttcgtgttc atgaatgatg ttttagctct gttggaagaa agaggcgtca
     5341 atgtagtttt ccgaaaagtc ggtaatttta tgacctcact agatatgcaa ggtctttctc
     5401 taacactgat cgacttgacg gagacacaat ggaaagattc tttagaaagc aatgtacaga
     5461 cgatttcatg gtaaaaaatc tcaagataaa agggtgataa tatgaaacta accgttcaag
     5521 aaatccaaga atggctcacg caatttgcag aggcaatcaa tcagaacaaa caatatctca
     5581 gtgatctaga taccccgatt ggcgatggag accatggaaa taacatggga agaggggttt
     5641 cagcttatga agaagctttt caagccgatc atcctgaaac aatcagcgat acattcaaag
     5701 tattctctat ggcgatgata tcgaaagtcg gtggggcttc tggtccgtta tacggatctg
     5761 cattcatgaa catgatgaaa gcgacaaaag aggtcgattc tattgattct caagaaaaat
     5821 taggcgaagt gatcgagcag ggaacagcgg gtattcaaag ccgaggaaag gcagaagctg
     5881 acgataaaac gatgttagat gtttggctgc cagtcacaga ggcattgaag gcagggaatt
     5941 taacaaaaga aatcattgac caggcaaaag aacacaccaa agtcttggta gctaaaaaag
     6001 gacgagcttc ctatctagga gagcgtgcaa tcggacatat cgacccagga gctgcttcta
     6061 gtgccatttt attttcaacg cttttagatg tgatcaatca accttaagtt caataaa
//
LOCUS       DACEDD010000112         6069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEDD010000112 DACEDD010000000
VERSION     DACEDD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.112"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..134
                     /locus_tag="IWO45_002921"
     CDS             <1..134
                     /locus_tag="IWO45_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0334344.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="IWO45_002922"
     CDS             complement(220..1314)
                     /locus_tag="IWO45_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0334345.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1503..2264
                     /locus_tag="IWO45_002923"
     CDS             1503..2264
                     /locus_tag="IWO45_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0334346.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IWO45_002924"
     CDS             complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IWO45_002924"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0334347.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3039..3794
                     /gene="fabI"
                     /locus_tag="IWO45_002925"
     CDS             3039..3794
                     /gene="fabI"
                     /locus_tag="IWO45_002925"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0334348.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3904..4917)
                     /locus_tag="IWO45_002926"
     CDS             complement(3904..4917)
                     /locus_tag="IWO45_002926"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0334349.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5082..6002
                     /locus_tag="IWO45_002927"
     CDS             5082..6002
                     /locus_tag="IWO45_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0334350.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1863 a         1205 c         1081 g         1920 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa
     1381 tggtctacac cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg
     1441 tattttccct ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag
     1501 ttatgaagaa aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg
     1561 ctggttgttc tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta
     1621 catctactgc ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac
     1681 aattgacgaa agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca
     1741 caatcaaaat caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac
     1801 atgcaaaaga cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga
     1861 tccaaggtgg cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat
     1921 ttgaaacaga gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct
     1981 cacaagatcc taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc
     2041 atgatggtct tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg
     2101 gttatccttc actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg
     2161 tcgtggatgc tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa
     2221 aaatcgataa aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa
     2281 tcctgcatag atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa
     2341 gtaagcttcc ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg
     2401 aacgtgaact cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta
     2461 ccgactgatc ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca
     2521 actttttgac ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac
     2581 tcttctgatt ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca
     2641 cccggcatca ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca
     2701 ttttttgtcg caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa
     2761 ataggataac gattaggaat caattccatt acttcagtag cagataatac gtaagacata
     2821 attaaactcc ttctttcagg caattgccat tttcattact ttgactatca aagtatcata
     2881 ttcctaaata tttagcaatc tttttttaat ttaagaaaaa atagtttgac aatcaaaaca
     2941 ttttgactaa taattaagaa atcaagaagt tattttagct attattctct atttttttac
     3001 gactatcaaa tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa
     3061 aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa
     3121 tgttgaaaca aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat
     3181 tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa
     3241 gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc
     3301 atgcagttgc ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag
     3361 acggctatca attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg
     3421 cacaaccgtt tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac
     3481 gcgcaatccc gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc
     3541 gttatttggc agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg
     3601 caatcaaaac attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg
     3661 aatctcgtac gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt
     3721 tcttaatcag cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg
     3781 tccatctttc ataaactttt gttgcattct ttaaagtaaa acatataaaa atcaggaaaa
     3841 aactttttaa agtctcttcc tgattttttc tttttacgtt tttttcaact cattactttg
     3901 aaattattcc acgacttcaa tccagccttc tggtgcttct atgtctccaa attgaatacc
     3961 tgtcaattca tcatacaacc gttttgttac tggtcctact tccgtttcac tataaaatac
     4021 atgaagattg ttattgtgat aaatcccacc gattggagaa ataacagccg ctgtaccaca
     4081 tgcaccagct tctgcaaatt gatctagatc atcaatgaaa atatctcctt caattgcttg
     4141 cagtcctagt cgttcttcag ctaactgcaa caaggaatat ttcgtgatac ttggtaagat
     4201 agagggcgat tgcggagtga tgaattttcc gtcttttgta attccaaaga aatttgctgc
     4261 acctacttct tcgattttcg tatgtgtggc tggatcaaga taaacacaat ctgagtaatc
     4321 cagttctttt gcttctgttc ctggaagtaa actcgctgcg taatttccgc cgacttttgc
     4381 tgcaccagtc cccttaggag cagcacgatc gtaatcagat acaataaaat tcgttggttt
     4441 tagtccacct ttgaaatacg ctcctactgg cgtacagaag acgacaaata aatattctgg
     4501 agctggatgg acaccaatgt tttcaccgac cccaatcagt aaagggcgca agtataatgt
     4561 agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct tttaccactt gcttgacagc
     4621 atcaacgaac atcttttcag ggaccattgg catcagcaat cgtctagcac tttgattcat
     4681 ccgtcgacta ttttgttcag gtcgaaagag attgaccttg ccgtcttttc gacgataagc
     4741 ttttagacct tcaaaacatt gctgaccata atgaagcgct ggagagcctt catgaatcgt
     4801 caggtaatta tcttctgtca attctccttc ttcccattgt ccgtctttcc atttggcaat
     4861 aaaacgccaa ggagttttga tataagaaaa acctagattg ttccaatcga tttgcatata
     4921 cttaccacct tcgttatttt ctccacattt tatcattatc catattattt gtcattttat
     4981 tttcagcttt tttccttcat tatagttatt tttgctattt ccttttttga aaacttcttg
     5041 gtacaataca gaaagaatca aattttagaa aagaggattt tatgttttca ttattagcaa
     5101 ctgccggtac gacagactct tctggcgaga cattgacaga tcaagctgtc agcaaagtaa
     5161 atgcgtttca gcggttttgg gacgggattg attgggacgc cattttggct gcctttatcc
     5221 aaaaaagttt atatctgatt tttttgatcg tccttttttg gattcttcac cgtgtgggaa
     5281 aatatctgat tgataagtct tttaatcagt attctaaaaa agccattcta aacgagagcc
     5341 gcttaaagac tttacatagt cttttaaata ctatttttca ttataccctt ggtttctttt
     5401 ttatctatgc aattttatct gctatcggtg tgccgatcgg atcacttttg gctggtgccg
     5461 gaattgcagg tgtggcaatc ggtcttggtg cccaagggtt catgagtgat gttattaccg
     5521 gattttttat tatcatggaa caacagatgg atgttgggga ctacatcaag ctagcaaact
     5581 taacgattga aggaaccgtc gtatctgttg gtatccgtac ccttcagctg aaagcaacgg
     5641 atggtactgt ccatttcatc ccgaatcgaa acatcaccac gatcagcaat ctctctcgtg
     5701 caaatatgca agtactttta gatattcgga ttgttcctga agaaggatat gacaaaatct
     5761 atgagatcat tgatcgagta aatcgtacat tagctgaaaa ataccaagaa gagctccaaa
     5821 ctgaaccttc tatctttgga ttagtggatc ttggaaacag caattttgcg attcgtacgg
     5881 tctgctacgc attgaacgga aaacaatatg ccttaaaaga agagttcttg agctcctatg
     5941 tcaaagaatt gacagcttct ggtttcacga ttccgaacag tccaattgct gtcggaaaat
     6001 aatttggaca aacgatgcaa cgaatctcac taaaaagagc gtgggacaaa agtaaagaac
     6061 acttttgtc
//
LOCUS       DACEDD010000113         5862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEDD010000113 DACEDD010000000
VERSION     DACEDD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.113"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO45_002928"
     CDS             complement(99..1229)
                     /locus_tag="IWO45_002928"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0334351.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWO45_002929"
     CDS             complement(1878..3080)
                     /locus_tag="IWO45_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0334352.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWO45_002930"
     CDS             complement(3140..3343)
                     /locus_tag="IWO45_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0334353.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWO45_002931"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWO45_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWO45_002932"
     CDS             4880..5692
                     /locus_tag="IWO45_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334354.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1209 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa tt
//
LOCUS       DACEDD010000114         5801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEDD010000114 DACEDD010000000
VERSION     DACEDD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.114"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..777)
                     /locus_tag="IWO45_002933"
     CDS             complement(25..777)
                     /locus_tag="IWO45_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0334355.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1141..3807)
                     /locus_tag="IWO45_002934"
     CDS             complement(1141..3807)
                     /locus_tag="IWO45_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0334356.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4148..5599
                     /gene="cls"
                     /locus_tag="IWO45_002935"
     CDS             4148..5599
                     /gene="cls"
                     /locus_tag="IWO45_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0334357.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1677 a         1227 c         1016 g         1881 t
ORIGIN      
        1 taataaaatc ataaaaagaa cccttcacga gtcgtgaaag gttctttttt tatctgcgga
       61 ccaaaggagc agtctatagg tcagcttagt cgtactaagg atttctcgtt tttcactgaa
      121 tagtttgcga atgtacctgg tcacaacagg atcttttgcg tacgcgtcat agtatagctt
      181 catcaattct tcagtaattg attcctgtgt aaaataagta aaatcttttt tcttcacttt
      241 gaatccttca tcaattaagt atgtttcaaa ttgatccatt acaactggcg atgtattagg
      301 atcttcttct atcattccta gttgcttttc gataggataa aaaacatcat cgattgattc
      361 cactactctg attatcacgc accatttgct tgactttttc atcaattttt ctaattgaat
      421 aagatgtacc aatgctggat ttgctgctgt aaaaaccatt tcatatgtct catttgattg
      481 gataaattct tctaacgact gtttgataaa ggaaacattt ggcaactcat ttttttctgc
      541 ttcttttttt gcattttcta gcattcctga agaaaaatcg atcaaatcat agcgattaat
      601 ttcttttgct aaggaaagaa atctgccaga tcctccagca acgtctgctg ctgtttcagc
      661 aggcaggact ttctccgttt tcagataatt ttttacatct tcagcaatag ttacttgcga
      721 ttcttgctgg atttcataat attcttcagc aaattcatcc cattcttttt cattcatttg
      781 atttcctcta attttttgtt atatgttttc acttttcgga ataaatcatt ctcctctttt
      841 atctattcta tctcttttga tgaataataa aatgattggc tttccgtcta tcataaataa
      901 aaaaagggac tgtgaccaaa gtcgtgtcaa agtccctctt tccgaataaa cagtgtccaa
      961 tcagatgacc ctcagtatta gctcaaaatg ctgattttac aacttcttcc ttaaacggtg
     1021 ttctcctgcc aactcttcgg tcacaagtca caatattcaa caaacatgag aagctgtttg
     1081 ttgaaattgc accttgtgac tcagaattaa acggcaggct cacaacctcg ttgattcgtt
     1141 ttatttattt ccttgtactc gtacaatgat caatttagtg atttccatca aaacaattgt
     1201 cacaagtgcg attcccatac atgtaaagac ttctgtcaat ccaaatgctg atgggataga
     1261 gaagacttct ttcagtcctg ggatcattgt taagcagtaa agcgcgctac atacaactac
     1321 agctagtaag acacttctgt tagagaagaa ccctgcttgg atagctgttt gtgtgtttga
     1381 acgtgctggg aatgtttgca atgtccgaca ccaaatcaat gttgaaaatg acatggctac
     1441 accgaattca ggtgaatgct gcatgccaag ccattgagca aagataacag caatcccaat
     1501 cagcacacca cgatacgtta cagaaatcaa tgttttgtcc gtaaagattc ctgaatttgg
     1561 atctcttggc gggcggttca tcgtagcagg ttcagctttt tctgtaccta atgcaattgc
     1621 tggcagtgaa tcattgacta aattgatgaa caacagttgt aatgctgtaa atggattgga
     1681 ccagtcagca actaaggcaa agatgatcgc aatgatcgca cctaaatttc ctgcaaatag
     1741 ataagaaata gctttcttga tattatcaaa cacattacgt cctacttcta ctgcgctgac
     1801 aatagaagca aagttatcat ccgtcaatac catcgctgct gcatcttttg ccacatctgt
     1861 acccgttccc atagcaattc cgatgtctgc ttgtttcaat gctggtgcgt catttacacc
     1921 atcacctgtc atagctgaga tatgaccttt attttgccaa gcacgaacga tacgaatttt
     1981 attctcaggt gaaacacgtg cataaacagt gactttttct aaaacttcgt ttagttgatc
     2041 ttctgtcaat tcatctagtt ccgtcccggt caaagccagg tttccttctt caaaaatccc
     2101 gatttgtttt gcgatagcaa cagctgttgt tttgtggtca ccagtaatca tgattgtttt
     2161 gattcctgca cttcttgctt ctgttaccgc ctgagtcact tcagcacgtg gaggatcgat
     2221 catagcaagc aaaccaacaa ggatcaaatc gtcttcatct tcaaatgata aatcaaaatt
     2281 atcaagtgga cggtaagcaa aagctagtac acgtaatgcg cgtgttgaaa aagcttcatt
     2341 ttgctcttgg aattttttca tcaattcatc cgtcatcgga acgacttcac catttagcaa
     2401 tactttttta ctgcgttgga agacgatatc tggtccgcct ttaacaaaca agtaattttt
     2461 gttatcaatt gtgtgtccag tagacattaa ttttcgatct gagtcaaaag gaagttctgc
     2521 ttgtcttggg tagttgtttc ggatttcctg ataaggctgg tttaattttt cactaaagtc
     2581 gataaacgct acttccgtag ggtcgcccaa tttagtaccg tcttcactga tcgatgcatc
     2641 attcgctaaa acactgattt cgatcaagcg acgttcgtca gctgtccatt ttgaagggtc
     2701 atttgtgaaa tccccagtat cgccattacc taaaaagtag tcaacaactg tcattttgtt
     2761 ttgtgtcaat gtaccagtct tatctgtaca gatgatactt gtcgaaccaa gtgtttcaac
     2821 tgctggaagc ttccgaataa tggcatgacg ttttgccatt ttattcgttc ccaaagataa
     2881 cacgattgtt acgatagact gtaatgcttc tggaatagca gctacggcta cggctacggc
     2941 aaacatcact gcgctgacga tatctgcagt catatctcct gtaccttctc caagataaat
     3001 acgaaacagt tggataccta aaatcaataa agacaatgcc aaaatagcca aacttagttt
     3061 tttactgaat ttatccagac ctcgttgtaa cggagtgact tgctcagttg ttgattccaa
     3121 aaggccagca acttgtccga tctccgtatt attcccagtt gaagtcacta agaatttacc
     3181 gcgtccgtaa gttacgatcg ttccactgaa taccatattt gtacgatctc cgattggtac
     3241 cgtacttgtg atatccgtta cttctttttc tgcagaagtc gactctcctg tcagcatacc
     3301 ttcatctact tttaatgatc ccgcatctag taaacgtcca tcagcaggta cataatcgcc
     3361 tgcgtctaac aacacgatat ctcctaccac aagttcgttt gccggaatcg ttttttcctt
     3421 accatcacga atcactcgag catcgggcgc tgaaaggttt ttcaacgctt ctaaagaacc
     3481 ttctgctttt tttgtttgga caacactgat tatagaattc aacatcaata ctgcaaaaat
     3541 aactaagaat tcgatgtagt cacccatgac catctgaatc agtgcgacaa tcagcaatac
     3601 gataaccatt gcatctttga aagtttctaa gaacaatttc caagttggct tcttcggctt
     3661 ttcttttagc tggttcaagc catctctttc taatttttcc ttcgcttgcg tggtgctcaa
     3721 tccatcggtt gagctctgcg tttgctgcat caacgagtcg atcgtttctt tatagaccgc
     3781 ttgttgtgct tgttccttgt tttccaaatt tcatccccca ttttttgatc ttggccctag
     3841 tcctgtctga aaaaaaagag acctaagaca aatacgcatt tgtcttaggt ctcaccattt
     3901 catacaatac cagaaaacca tttggttaac ttactgttga tattatcgca acaaacgttg
     3961 ccggttactc ccctaaggag atattcaact gtactaatat cctaccttat taacctacaa
     4021 aaagcaacta ctttagcccg atttacctac attttcatga aaggagtgaa aagtatattc
     4081 accttggtag gcttctttct tctatcatgc tatactaatg ttattgtatg taaggaggaa
     4141 catttttgtg gtatctagta ttataaccgc cctttatcta ttaaatgcac ttattgcttt
     4201 ggtggctatt ttgatcaaac cccgagatgt agcagccatt tgggcatggc tcttagtatt
     4261 gtttgccctt cctggggtgg gctttgtatt atatttattt ttcggacgtg gattaacgga
     4321 taagaaaaaa ttctatctcc gacaaagtga cttgaaagaa ttagaaaact ttcagtcttt
     4381 tagaggagat accattgaac attacgatcc tgacatgggc gataaagaca aacagcaatt
     4441 tgttgacttc ttctcttcat taaatcgtat gccgctgaca agaatgaatt ctgtcactct
     4501 tctcacagac ggacaagaga aattggattc actgcttcaa gatctaaaaa aagccaaaca
     4561 ttcgatccat atcgaatatt acgcatttgt gacagataat atcggccagc aagtcttaca
     4621 tgttttagaa gaaaaagccg cagaaggcgt ggaagttcga atattatatg atgcatttgg
     4681 ctctcatggc acaaaagcaa aagatttcaa tcgtctaatc aaaaatggtg gacatgtcca
     4741 tacatttgtt acctcacaaa gggcattact tcgtttccga ttgaattacc atgatcaccg
     4801 aaaaatcgtt gtgatcgatg gaaagattag ttataccggt ggtttcaata ttgccaatca
     4861 atatgtaaat acaacaaaaa aattcggcta ttggcgcgat acgcatatac ggattttcgg
     4921 tgccgcttct tctttgctcc agcttcgctt cttaacagac tggaacgtct cggtacctga
     4981 agaaaaaaag gtcggctatc atttgaatta tttctttaaa aaagcagatc gagatgaatc
     5041 taagcttgct gatacatcca tccagcttgt ttcaagcgga ccgaataacg aaagggaaca
     5101 aatcaagctt tcatttatca aattgattac ttctgctaaa aaacgtgttt ggatacagac
     5161 accttacctt gttcctgatg aaagtgtcat tgctgcttta aaaatcgcaa ctgcctctgg
     5221 tgtagatgtg aaaatcatga ttcccaacaa accggatcat ccttttattt atcgagcaac
     5281 acaatattat gctcggcagc tgatcaagga aaatgtacaa atccttgtct atgagaacgg
     5341 cttcctccat gcaaaaacat tgataatgga tgatgaaatc tgcatggtag gttcagcaaa
     5401 tcaagatatt cgtagctacc gattgaattt tgaaacaagt gctgtcattt acgatcctga
     5461 gtttttagaa gaacttgcta ctcagttcaa agaagatgag acacattgtt catccatgac
     5521 aactgaaaca gtcaaggaaa tgtctaactg gctattattc aagcaacaaa tttctcgatt
     5581 attttctcca atcctataat ttttttattc aagtagagaa aaatatacta agattaaagg
     5641 gactgtgaca aaaatcttgt cacagtccct tcttccgaac aaacggtgtt ctcctgccaa
     5701 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgtttttt gaacttgttc
     5761 cttgtgactc ggagctggac ggcaggctca cgacctcttt c
//
LOCUS       DACEDD010000115         5391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEDD010000115 DACEDD010000000
VERSION     DACEDD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.115"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1421)
                     /locus_tag="IWO45_002936"
     CDS             complement(<1..1421)
                     /locus_tag="IWO45_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334358.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFG"
     gene            complement(1408..1626)
                     /locus_tag="IWO45_002937"
     CDS             complement(1408..1626)
                     /locus_tag="IWO45_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334359.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENN"
     gene            complement(1619..2584)
                     /locus_tag="IWO45_002938"
     CDS             complement(1619..2584)
                     /locus_tag="IWO45_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ0334360.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            complement(2774..3058)
                     /locus_tag="IWO45_002939"
     CDS             complement(2774..3058)
                     /locus_tag="IWO45_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334361.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(3071..3364)
                     /locus_tag="IWO45_002940"
     CDS             complement(3071..3364)
                     /locus_tag="IWO45_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0334362.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            3433..4197
                     /locus_tag="IWO45_002941"
     CDS             3433..4197
                     /locus_tag="IWO45_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0334363.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            4248..>5391
                     /locus_tag="IWO45_002942"
     CDS             4248..>5391
                     /locus_tag="IWO45_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0334364.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
BASE COUNT         1804 a         1029 c          766 g         1792 t
ORIGIN      
        1 ccgaaattct tttctggttt tcgatcttct ccttctccgc tgtctgatcc atttccattt
       61 ccgcttcctt tagcacgatt aaatccattt gatccttttc taaacagcat tgcgcccata
      121 ccaaaagcag aagcaagttg cccataacct tctttgagtc caatatcaac accaaagtat
      181 ctcatgactg ttccactgcc tttgattaaa acaaaaacgg cacagatata agcaattgtt
      241 tttataaaaa tatttgttga gataccttgt cctgagttca gaaaattgat aaacatagca
      301 aacatggata gtccaaatcc ttgaaagcct acagtcaaat agcactgtag aatatctgat
      361 agaacaactt ttgaacgctg tcctgtttct aagtcagtag caaatactaa aactccgagt
      421 acacgtttaa atgctaactc gagtattgca gtaataatca caaaggcgct aaaaacatag
      481 gctattgcta aagctatcag cccaattgaa atgccccata gattcgcttg atagcgataa
      541 tatcctgatt tgataccatc aacaaaggtt gacaaaaaat tatctgaaaa tttagtagca
      601 acaaattcat tatcagaatt ttcaactaat tcataacgca ggttatcagc tgttgagttc
      661 tctttttcca ttttatcaat tacattatct gtcaatactt gcgtaagatc cgttttccaa
      721 aaattttctt ttgttagatt gttccgatca ttcgttttat cgatcatgga atattgatcc
      781 gttttattga tataagctaa atcagtaacc ccttgttcaa tcagcgacca tgccacaccg
      841 ccatcttgat tgttaatact agttgcgtca ttataaagag tctttgcaaa cattattcct
      901 gaaagttttt ctgttgatcc aatcaaagtc ggcattaaaa gaattaatac tatcgacatt
      961 aaaacattga ttccaacaga ttttagatca accgttcctt ttccagtcac cattttataa
     1021 ccaataatca ctagtgaaag tatcattaag ccaacaacaa tagtgcttaa aactgactga
     1081 tagactttgt tcaatccagc actttccaca aagctaaaca aatctaacac ttcaggaatc
     1141 atattttgaa taccatctac aattgcaaac atcgatttta taacagacca ccctatccac
     1201 aatccaaatt gactgtaaaa atttccgtta tattttttta aatacggttg atagacagga
     1261 ttattcaaaa attcagtttg actttccgta tcatcgaacc aattaaaaat gcttgcatga
     1321 gcaacattac cggtgaaaat aacgataaac attaaagcaa gtaagctcag agctaaccag
     1381 tttattttcc taccagcaat ctttttttta attattttca ttgttacctc cctcagccca
     1441 tttttttagg ctctcagaaa gcttctgagc catttcttga ttaattatga tatgagttac
     1501 acaattgtca ttttcagctc ttaaatcgag aaccacggtg ttaaagcaga tactctcgat
     1561 taattcgaga gtatctacat tttgatcgag acagcagatt tggtctatta gacgattttc
     1621 aatcatataa cacctctctt gtttgagtaa caaagttatt atatgctctg gaacttacag
     1681 tatttttgca gttatataaa gttgtaatcc agtaaagatt gagattgtgt atctcatgat
     1741 caattgcgtt aaatgaatca atagcagatt caatatcaaa ttgattgagt tgttctactt
     1801 tttctatcac ttcatttgca gatttttctt ctctatttat cctgtatgtt ttgctctttt
     1861 cagagtatat tgattgaatt atattaacaa tatcttcaac aattcttttg taacaaggat
     1921 tggattttag cgcattatat ccaatttgat tctttatttt ttgtctcata atttcgactt
     1981 ccatgtcatc cgatagattg atagattgat cttccctaat ctcacctaaa ctaaccttac
     2041 ctgacctact ctgatctgaa ctaacctgac ctaatctaac ctgggcatgc ccatggtcgt
     2101 cctttggtag accaacggga taccattttt caatcaaatc atttcgaagc atttccgttt
     2161 gacctgcttt taaaattttt tcaatactaa tttgttttaa tcttgtaaaa aagtctggat
     2221 tactatttaa taatttaaat cgatatatag agggtgtata tcttgtagtt cttaacgttt
     2281 gattttgcag ccaatgggga agaaatacta ataaatcttc attcagcata tagagaaatt
     2341 ctttagctaa aagaattttc acgtcatcat caggggttcc aagcatccgt acatatggat
     2401 atgcttcgac aatcccatca tcgtctgcgt ttacattcag atatacatat aataattttg
     2461 ctgaatccga catcttcata aaactcgcat tatttaaaaa agtttttgac aacatacgtt
     2521 tttctgccat aaattccaca cttcctcctg tcaatgttaa aatgtagtca aataaagttt
     2581 tcacagaact ttattattga gtgttcttcc ggtctgcaaa ctgtaaggag cactcttttt
     2641 ttgtatattc accttttatt aaatcaaata gaaaataaat cttgatttct tttaaactca
     2701 actttgcttc ttcatcatcg ttcaaacaaa cacttccttt ttttcatatc aaaagcacac
     2761 cacgcaactt tttttatatt tcatattctt tgataaatct tgctaactct tcttttctga
     2821 taaattgaca gtctccgatg atactcacct ttaaaccacg ctgattaatg aattttttta
     2881 gtgtacttct actacaactc aaatattcac acgcttctcc aagccggaaa atttctttgc
     2941 ttaaagatgt ctcaatacga acttgatcaa cagcttcctt agcaacttga taaaaatatt
     3001 tacgtaatga ttgatcaaga tctggcggta gaataacttg tacactcgat acatccatga
     3061 tgcccactcc tcattcgtct tttttcgtct tcaaatcact aaaaaaaata tccacaatat
     3121 ctaaatgaat tccatcttta tgaaatagct ttacgattct ttctatttca taatctttaa
     3181 acggcaccct tcctttctct ttgttacgat acgttagttc cgatatttct aataactccg
     3241 caagttcttt ttgtctatat ccacagaacc tgcggtattt tctaactgga gagtccaacc
     3301 ttaccacctc gtcttttttc gtctttacca acgatagaat agcatttatt tcattgtccg
     3361 tcaatactta cccgtcgttt ttcgtagatt ccattccgtt tttgtgttac attactgtcg
     3421 gagggatata atatgaacct taataagaca tcagtaggaa accgtattcg tttagttcgt
     3481 aaagaactcg ggctttccat gaaagagttc ggaaaaaaga taactcctac cgccgcagat
     3541 agtattgttt cgcgttggga gcgagggatt agcttaccta ataacgacag gctaactcaa
     3601 attgctaaaa ttgctcataa agatattaac tggttattat ggggaacttt taaagaattt
     3661 atttcatcat tgttagataa agaaggatac acaaattttt tgaatgattt tcctgaaaca
     3721 gtagatacta tttataatga tttaatgcct atttcggaaa atttacagtt tcctgattac
     3781 gaaaattcta ttttacgact attcaataag atatacttac ctatattcaa tgattactgt
     3841 ctagacatta ttgaactgga tttggaggac tttataaata agagacattc catctcctct
     3901 gacaagttta aaaataggtt tttatcctcc tttcatgaag gagtatctaa cagcggatat
     3961 aaatacggcg atacggattt aatttataaa tatggaattg cattacttga gaaatttgac
     4021 gattcttata aattaaataa tccatatgag tcaatagatg cgtatttttc tgccatgact
     4081 gtaaacactg aagataccga aaaacttata tataccattt gtaaatcgct tggcattcca
     4141 ttccaaaaag gaagcaatac cgcacagatt ctttcaaaaa accacaataa tttttaattc
     4201 attttttcta tcaaaagcac gccacgcaac tgataggagg aaaaacaatg gcaacaatta
     4261 agcaatatga aaagaaagac ggaaccaaag cttggctatt tcagacctac cttggaatca
     4321 gtccattaac tggtaaagaa gtccgtacta cacgtagaaa ttttaaaaca aaaaaagaag
     4381 ctcaaatcga gcttaataga ctacttgtag actttgaaga gaacggactt aaaaaggaaa
     4441 caaacgaaac tttcaaagaa gtgtatgatc tctggtatga gagctataaa actactgtaa
     4501 aggaagtcac tcaattaaaa attgagatta agtttaataa atggattctt ccaaaatatg
     4561 ggcagtttag aattaaagaa attactacaa aacatgttca gaagatattg aatcagtggg
     4621 ctaaaacaac agatcaatat aaagtcctac attctactgc aagccgtatt ttcaaatatg
     4681 caatgacttt agggataatt actaaaaacc cctgtgaact tgtaatcatg cccaatagaa
     4741 acgttgaaaa aaagaaagat actgtcaaaa tttattcacg ggatcaacta gaaagtctct
     4801 tccaatactt ggaatcaaaa agaagcacct atagagccga ctacgataaa acgctacttc
     4861 gctttctatt ttttagtggt tgtcgaatta gcgaagcttt agctttagat tggtcagata
     4921 ttgactttga ttcaaacacc gtcactataa acaaaacctt aagccaaaca aaatatgggt
     4981 acaaaatttc aagtccaaaa acgagaaata gctatggttc tctatcttta gacaataaaa
     5041 ccattaattg gctaaagaaa tggcaaatca atcaaaaaag atatatgtta acccttggta
     5101 ttactgatcc aacaatgatt ttttgtggaa tctacaagga aatcattact aaacacgcta
     5161 tttattctcg tatgataaca atctgcaaga attctggtat tccatttcta ggtaaccatg
     5221 taaccagaca cactcatgct agtatgctgt tagagtcagg agtctccatg aaggaagttc
     5281 aagaacgcct tagacactca tcaatcaaat taacaatgga tacctatgct catctaacta
     5341 aagaaaccaa agaaaaaacc gttgaaaagc ttgcagagca cctaaatttc t
//
LOCUS       DACEDD010000116         5341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEDD010000116 DACEDD010000000
VERSION     DACEDD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.117"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWO45_002943"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWO45_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWO45_002944"
     CDS             complement(546..740)
                     /locus_tag="IWO45_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334365.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IWO45_002945"
     CDS             2092..3132
                     /locus_tag="IWO45_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0334366.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IWO45_002946"
     CDS             3956..4288
                     /locus_tag="IWO45_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0334367.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="IWO45_002947"
     CDS             4907..5248
                     /locus_tag="IWO45_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0334368.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DACEDD010000117         5191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEDD010000117 DACEDD010000000
VERSION     DACEDD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.118"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            137..748
                     /locus_tag="IWO45_002948"
     CDS             137..748
                     /locus_tag="IWO45_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0334369.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IWO45_002949"
     CDS             821..1750
                     /locus_tag="IWO45_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0334370.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IWO45_002950"
     CDS             1908..2645
                     /locus_tag="IWO45_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ0334371.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..3871)
                     /locus_tag="IWO45_002951"
     CDS             complement(2693..3871)
                     /locus_tag="IWO45_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0334372.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..3977
                     /locus_tag="IWO45_002952"
                     /pseudo
     CDS             3894..3977
                     /locus_tag="IWO45_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4098..4499
                     /locus_tag="IWO45_002953"
     CDS             4098..4499
                     /locus_tag="IWO45_002953"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334373.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5191)
                     /locus_tag="IWO45_002954"
     CDS             complement(4706..>5191)
                     /locus_tag="IWO45_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0334374.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1641 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta a
//
LOCUS       DACEDD010000118         5177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEDD010000118 DACEDD010000000
VERSION     DACEDD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.119"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(718..3867)
                     /locus_tag="IWO45_002955"
     CDS             complement(718..3867)
                     /locus_tag="IWO45_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0334375.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="IWO45_002956"
     CDS             complement(3880..5079)
                     /locus_tag="IWO45_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0334376.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
BASE COUNT         1443 a         1063 c          836 g         1835 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgc
//
LOCUS       DACEDD010000119         5157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEDD010000119 DACEDD010000000
VERSION     DACEDD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.120"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(117..1079)
                     /locus_tag="IWO45_002957"
     CDS             complement(117..1079)
                     /locus_tag="IWO45_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0334377.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="IWO45_002958"
     CDS             complement(1081..2520)
                     /locus_tag="IWO45_002958"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0334378.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="IWO45_002959"
     CDS             2705..4651
                     /locus_tag="IWO45_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0334379.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4915..>5157
                     /locus_tag="IWO45_002960"
     CDS             4915..>5157
                     /locus_tag="IWO45_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0334380.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1660 a          872 c          853 g         1772 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     4741 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa
     4801 aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat
     4861 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
     4921 tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg
     4981 acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc
     5041 attgacttct tcaaacaata ccaagcagat ttagcaggga ttgggattgg ctcatttggg
     5101 cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DACEDD010000120         4864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEDD010000120 DACEDD010000000
VERSION     DACEDD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.123"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1221)
                     /locus_tag="IWO45_002961"
     CDS             complement(<1..1221)
                     /locus_tag="IWO45_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0334381.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMY"
     gene            1282..1857
                     /locus_tag="IWO45_002962"
                     /pseudo
     CDS             1282..1857
                     /locus_tag="IWO45_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2038..3231)
                     /locus_tag="IWO45_002963"
     CDS             complement(2038..3231)
                     /locus_tag="IWO45_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0334382.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3315..3518)
                     /locus_tag="IWO45_002964"
     CDS             complement(3315..3518)
                     /locus_tag="IWO45_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0334383.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            3775..4017
                     /locus_tag="IWO45_002965"
     CDS             3775..4017
                     /locus_tag="IWO45_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334384.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(3939..4172)
                     /locus_tag="IWO45_002966"
     CDS             complement(3939..4172)
                     /locus_tag="IWO45_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334385.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4179..4610)
                     /locus_tag="IWO45_002967"
     CDS             complement(4179..4610)
                     /locus_tag="IWO45_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0334386.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1326 a         1110 c         1004 g         1424 t
ORIGIN      
        1 atacatttct acaaaaaaat agtgattctg ttttgaaaat tcgattttta gttctgcatt
       61 ttcgtaaaca tccaacattt ttttcgcact gtatagacct aacccgccat tttttttaga
      121 ggtttttcct cttttgacca tctccgatag cgtcaaattc gttggatttg ccttgttcgc
      181 tatactaaaa cgtgatccct ctttttcttc cagcagattg atcgtaatcg gactctgatc
      241 ggctacacga tgatcgaatg cgttagaaat aaaaatagat aaacagcgaa taaaaacaac
      301 cagatcaatc gggattgttt tcacttcgac aggaataact acttggatcg ggattttctc
      361 cgattctgct ttttctagaa attttaaaaa gacacccctg acagctgggg atacaatatt
      421 tgttagctgt tgtatttgcg gtttttcaaa aatagctgca gaatgatctt ctaatatttc
      481 taaaaattcc aacgtttctt ttttattttc ctgaagagta atttttaatg aaaacagtag
      541 acttttataa tcatgtttga attcctctag atgatggtaa tattcttcat ttatttgata
      601 cagttgaagc gattcctgat gttgtctttc ttttacttgg aaactgatca acataatgga
      661 aaaaaaaaaa gaaaaaaaac tgatgactat cgacaaaaga gatagaagca gataattaga
      721 tgagcggaag tgaacacttc ccaataccaa gtaaatcacg ccccagctta ataaaataca
      781 aatacctcca aaatagtgtt cagttggata agtaaaaagc aagttgagaa atctcgtctg
      841 tttgtctata ctttttacta atagatagaa aataaatgcg ataagtagtg caaaaaaact
      901 tgctgttcca agatgtctct ttcctaaata atccatcaga tcaaaaacca gaaaaaagct
      961 gagtccagtg atagcaaaaa aaagattact gatcaaacag ctgataaaca ggtttttcgt
     1021 agtaaaataa aaaagaaaca tcaataataa gccaactagg taaggaaagc tcactttagg
     1081 gataggaagt aaaagtgtca ccaccatcaa agctatacaa ccaaagctgt tttttactag
     1141 cagttgtttc gaaaagaata aatagatacc taaatctaaa atcaaagtaa aggtcaaata
     1201 aagtgtcact gttgcgacca tcttattcct ccattgtttt gtttcaaaat gtacacatat
     1261 ttttttaata ggagcctgca tatgaaaaag tattttatca tagaagataa ccagtttcac
     1321 agcgaatgga tcatcagaga attggtcaat catcatatcg agaatatcgt gcagctcact
     1381 tctatctctc aaatcaatca gttgatccag ttgggcacta ttgataatca cgatgttttc
     1441 tttatcgata tcgacttgaa gcaatcactc acaggtattg atgtcgccga acaaatacgt
     1501 aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt atacgaattt tgcagtagag
     1561 atcatcaatc ggaaaatcct tccgattgga tatatcagaa aagacaggaa tttatctgaa
     1621 caaattcgtc aagtgctaga acagatcaat tggaaagaac agcagatcgt aaatgtcgat
     1681 aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt atcctcggga aattttatat
     1741 attgaaagta tcaaaggttt gaaaaaccaa gttatggtca aaacaactac agaagataaa
     1801 ttagtgaata cttctttaaa acaattgaag acagagttag aaaagtatgc ttactttttc
     1861 ttaaaaatta tatgggattt tagattatct ttttagaagt tcttgaatgt cggtatagcc
     1921 cgtgtttccg ggcttttccg gcatttttga tattgttaga tacttacaaa tgttcttatt
     1981 acctctcacc atttccccct caaaagtagt aaatggagta gtaaaaccgg ccttgtgtca
     2041 ggctgccagc cgttccattt ctgcccttgc ggaatcgaat gtggcgtgtg cataatagtt
     2101 cagcgtcatg gtgatattgg aatgtcccat aatgtactgc aaggctttgg ggttcattcc
     2161 cgcattggct aaccgggtgc agaatgtatg acgcatagca tggggcgtcg tcgttttcgg
     2221 caacgcttct tcatggctct tgttgtactt cttcacaagc ctgccaaaca tcccgccata
     2281 atctaccgcc gtcatgggat agccgttctg tttgagaaac aggaaattgg catacccgtc
     2341 aataatgaac ggctttgcac ctttgcggtt ttttatcact cgctggaacg cctgatacac
     2401 ttcctcattc atggggattt ttctgacgcc gctttgggtc ttgggttcgt caatatagta
     2461 accgacgccg gtatttctca aaagctggtg gctcacgatg attacccggt tttcaaaatc
     2521 caaatctttg tccgtcagtc cgcacagttc cgaaatgcga agccctgtcc ccagcagaat
     2581 aacgattgcg tcataatgct tttggtaaac cttatcacct tgaataaagg acagaaaact
     2641 ttcttcctgt gctggtgtaa gaggcacctt tggttccgta tcgtcgtcca gaacgtcact
     2701 aagctggaag tcaaacgggt tttttcggat gcaatcgtcc tgtatggcgg tgtaaaaagc
     2761 cgctttcagg gaacgcttgt cgttattgat ggtcttataa gaaaggccct tttctttcat
     2821 ccgaagcgcc cattcctttg cgtcggacag cttcacactg tcaatcgggc agctccccag
     2881 ccgatcttgt tccagaatac gcatcagccg ttcccggccc agtttggtgt tgtgcttcac
     2941 attgccccgg tgcctgatct gcttggcgta gagctggcag acggtcattt tcttgccgat
     3001 ggtgtctatc ccgtcgtcaa ggtctttctg gatttctttc tccttttccc ggagtgaaat
     3061 atcctcccgt ttacctgccg gggtcttatc cgtaggcact aacttccatg agtaaacaaa
     3121 ctgcggtttt ccaaaggtat caacatattt gtaagcatat cgtccgtctt ttctctggct
     3181 ctctccagac cgcaaaacgc gatttttgct gtctcgtctt ttttctgaca tctcgtcatg
     3241 ctcctttcca tgacggaaag agccgcatat atgcttataa attattataa catatctgcg
     3301 gccctttttc actattagat tgtgtccaaa gtgtcaatta ttttctcaaa ctgcttgcgt
     3361 ttgatctgga tgcggttgcc attcataatg acccagccag aggccggatt ttcctcggcc
     3421 agcttacgca atttgttttc tccgatacgg aaatatttag acgcttcctc aatcgtcagc
     3481 gtgtattttt cccaaatagg cacatcatgg ttcataatgc agcccccttt cacaaattca
     3541 aaggtttgtg tgatttaggg gcatagacgg acggctgctg gcacagctcc acgggacttt
     3601 cacccccgcg tggttctcac gcggccctac ccattgcctg cgacgctttt aacgctcgga
     3661 ctgtggctat aagggagtat catagtcctg cctgtacgtc gtcgcccgca agccgccacg
     3721 ctcacattgc ggcgtggtgt tcctcgctcc gcttttcccg tagggaaaag tatcatggcg
     3781 caccagccgt ccggctcggc acagacagaa tgtgtccgta tgccctatga tgcgccgccg
     3841 ttatcttgcg ggcatattta tcaaggtgcg atcaggccac cgggaaaaca ccgaaacggg
     3901 gaaagggtaa gccgtttcat gctgccgggg ccggttcgtc agttcgtgac gatagcgtgg
     3961 atgagcttga tttccagacg gcgcttcatg gcctcgtcca gacacacatg cggatagccg
     4021 ttttcgtcat agagcgtccg ggtacacagc ttgttcatgt aaccgctgta atactgcaaa
     4081 acgcgctcta cggctccggc atccccagcg cgggcggcct cgatcaccga aaggggcaaa
     4141 agctcaatgc ccttgtagtt cgggttcttc atccgcgtct aacctccctt cggcatcaac
     4201 gcctttagct gtttccgcag ctccgataag gtatttgtcc tgtgccgctg aacggcactc
     4261 cgggacattc ccacaaggcc gccgatctca ccatctgcca tatccagaac acagtgcagg
     4321 attaaaatac tctgctccat gctgggcagc gcggcaaagg cttcggcaac aagctcattg
     4381 ttgatatgta ggtcatagcc atgcgacgaa aacgtaaagc tgtcgctggg gtagtgatct
     4441 actgtacaga gcttgtccaa atctgcctgt gacaaagagc ttagtgacac ttcacggtca
     4501 cggcggcgct tcatttcagc caagtagctt ttggcctcgt tacgcagtac cgtcttgcaa
     4561 aaagcatcga acatgcaccg cgcttcatag tcgcggggga tagcatccat cttctcaccc
     4621 cctttctgtg ggggacttag gtggttctct ccctttccgc taacaagact ttgacaaggc
     4681 cgccagctac ccgctaaata cctccttttt gaaaatttct caaaaaattt ttttggggac
     4741 agccgggccg caaaagacga caaaaatcgc ccggcaggtc gcaaacctac cgggcgagaa
     4801 aatgctttgc tatgctgctg atttacatgg tatagtacat actcataacc ggaatagccc
     4861 tgtg
//
LOCUS       DACEDD010000121         4745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEDD010000121 DACEDD010000000
VERSION     DACEDD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.124"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..104
                     /locus_tag="IWO45_002968"
     CDS             <1..104
                     /locus_tag="IWO45_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAQ0334387.1"
                     /translation="LLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            792..1613
                     /locus_tag="IWO45_002969"
                     /pseudo
     CDS             792..1613
                     /locus_tag="IWO45_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1756..1980
                     /locus_tag="IWO45_002970"
     CDS             1756..1980
                     /locus_tag="IWO45_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0334388.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            1995..2864
                     /locus_tag="IWO45_002971"
     CDS             1995..2864
                     /locus_tag="IWO45_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0334389.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2845..3579
                     /locus_tag="IWO45_002972"
     CDS             2845..3579
                     /locus_tag="IWO45_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0334390.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3612..4520
                     /locus_tag="IWO45_002973"
     CDS             3612..4520
                     /locus_tag="IWO45_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0334391.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4517..>4745
                     /locus_tag="IWO45_002974"
     CDS             4517..>4745
                     /locus_tag="IWO45_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334392.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYL"
BASE COUNT         1615 a          708 c         1043 g         1379 t
ORIGIN      
        1 ctttgctatt agttatggat ttgcaaagtc accttattga acaatacaac ttaaaagtta
       61 cacatgacat tttagaaaga ttgttgaatt acattagtga ataggagagt aacgttattc
      121 acagtattgg gtactgaaaa ttaacagcat acctcagttt ttcaaaaaac agcgaagcct
      181 atggatatgt tcccgtctac cgatagtgca aaaagtgtcg tggatatgtt tccgagaacg
      241 gacatacaca aatgacattc attctgtcct ctcaatccat gtggaagcag tatgtctgtt
      301 aacaagaaaa taattattgt tcgtcctgac gagttgttag actcgaaaag gataaacatc
      361 atttttagaa ccggtagccg atttttggct agcggttttt tgggctcttt ggcaaatagt
      421 ggtgattgaa tctcgcgcta tcctatggaa aaccgccttg tgtgaggggc tgcgagaccg
      481 cttcgatcct agaattgctg aagcacgaat ctagcgtcac tcacatttca tcttttgacg
      541 tagctgagac aaggtgtttt tcttcgatcc gctagtggtt aatctctttt tggtgcaaat
      601 atggtttaga atttctttgc atggcttaag ggagtggaga atcggatatt agtaggattt
      661 acatccacag ataaaaatag gcttgatttt aacagcgaaa ctaaaatacc ctacttaaat
      721 gaagtgatag attagatgtt gggttgttag gagatgctta aaaatcctat gcaatcaata
      781 gtttttagtg tttgttgact tgatccctaa atcgcctgag tactaaacgt tgagtgtgtc
      841 tgcttactgt cactagccag taagtgagac tgtagttaat ctctcatttt ccccagtatt
      901 atgttgaaaa tagccatgaa gcgattattt ctaaagagtt atttttgcag gctcaagaag
      961 aaatttatcg gagaagtaat atctacacag gagaaggcaa gaacaaaaga atttatagta
     1021 gcaagtacgc tttaagtgcc atcaccttct gtggatattg tggcgatatt tataggagaa
     1081 cctattggaa tattcatggc agaaaagaat ttgtctggcg atgcgtgact agaatcgagc
     1141 agggtcccga ggtatgtaag aaccgaaccg taaaagaaga tgaactctat ggtgcggtaa
     1201 tgaccgcaat taataagcta cttgctggtg gcaacaatat gataaagaca ctggaagaaa
     1261 atattcatgc tgtgattgga gaaaccacag aataccaaat ttcagagatt aataccctac
     1321 tagatgaaaa acaaaaagaa ctaatcaagc tggccaataa aggtcaagat tatgaatacc
     1381 tggtagatga gatcgatgag atgcgagata agcgtcagac tcttttagta gaagatgcct
     1441 ccctcagtgg cgagaacgag cgcatcaatg agttgattga atttatccgc aagaacaaat
     1501 tccgcacctt agaatatgat gataagcttg taaggaagat aatccagaac gttaaggtct
     1561 atgaagacca cttcgtcata gccttcaaac caggtatcga aatggaaata tgaaaaccag
     1621 aagtaaatag cccatgactc tgaagtagag ttgtgggttt tcttttaatt gctgattata
     1681 ataatatttt aatgactatt gcatttatca accaaatggt ttatgttatt cttagcataa
     1741 acttggaggt gctgtatgac tacggcagaa atgattaaag aactgtgtga gcaaatgaat
     1801 ataagtgttt ccgaacttgc tagacgtatt ggccagactc cacagaattt taataaaaaa
     1861 ttacaacgag aaacagtaac cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc
     1921 aagtttgtgc aggcatttat tttaccagat ggagatgaaa taaaaatatc taacgaataa
     1981 aggaggatgt ttatatggta ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt
     2041 tgccttttat agaaggcatt gtattagggg gctcacgtgc aagaagcacc catacagaaa
     2101 attctgatat agatatcgga atctattaca attcagactc atttgacctg acagcgatta
     2161 atcaaattgc tacagagttg gatgatgaga atagaaacaa ccttgttgta cctcccggag
     2221 cgtggggtga ttgggttaat ggcggtggat ggttagttat aaacgggtgt catgttgact
     2281 tgatattacg tgatataaaa cgggtggaac aaataatcaa agatacggag caaggaattg
     2341 ttactgccaa ttatcagacc gggcatcccc atggttatat aagtgctatg tatcgtggag
     2401 aattagcgat tagtaaaata ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc
     2461 aggcagaaat ttaccctact gctctaaaga aaagcttgat gaactttttt atatttgaag
     2521 ctgaattctc tttaatgttt gtaaaagcaa atgcgggggc agaggataaa tattatattg
     2581 caggccatgt ttttcgtatt atttcatgct taaatcaagt actatttgca tgtaataatg
     2641 cttattgcat taacgaaaag aaagctataa aactgcttga aacttttgaa tataaacctg
     2701 aaaaatatgc cgaaagagtt aatcatattt ttgaagtact cggtctttct ctttttgaat
     2761 gctacgacat gaccgagaaa ctttataaag aagtgaatga aattgtatcg gagataaata
     2821 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     2881 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     2941 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3001 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3061 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3121 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3181 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3241 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3301 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     3361 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     3421 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     3481 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     3541 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     3601 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     3661 aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt aatataccta
     3721 aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa ccgtttatat
     3781 ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata
     3841 tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca
     3901 ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata
     3961 aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg
     4021 atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat
     4081 tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta
     4141 ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg
     4201 gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata
     4261 tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca
     4321 tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg
     4381 aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca
     4441 tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag
     4501 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
     4561 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     4621 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     4681 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     4741 tcttt
//
LOCUS       DACEDD010000122         4744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEDD010000122 DACEDD010000000
VERSION     DACEDD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.125"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWO45_002975"
     CDS             25..696
                     /locus_tag="IWO45_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0334393.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWO45_002976"
     CDS             755..1195
                     /locus_tag="IWO45_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334394.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWO45_002977"
     CDS             1207..1875
                     /locus_tag="IWO45_002977"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0334395.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWO45_002978"
     CDS             1939..2562
                     /locus_tag="IWO45_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0334396.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWO45_002979"
     CDS             2618..3004
                     /locus_tag="IWO45_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0334397.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWO45_002980"
     CDS             3217..4608
                     /locus_tag="IWO45_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0334398.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1580 a          782 c         1011 g         1371 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttg
//
LOCUS       DACEDD010000123         4619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEDD010000123 DACEDD010000000
VERSION     DACEDD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.126"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            41..292
                     /locus_tag="IWO45_002981"
                     /pseudo
     CDS             41..292
                     /locus_tag="IWO45_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            385..1179
                     /locus_tag="IWO45_002982"
                     /note="aph(3')-IIIa"
     CDS             385..1179
                     /locus_tag="IWO45_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0334399.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1589..1700
                     /locus_tag="IWO45_002983"
                     /pseudo
     CDS             1589..1700
                     /locus_tag="IWO45_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1721..1804
                     /locus_tag="IWO45_002984"
     CDS             1721..1804
                     /locus_tag="IWO45_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0334400.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1929..2666
                     /gene="erm(B)"
                     /locus_tag="IWO45_002985"
     CDS             1929..2666
                     /gene="erm(B)"
                     /locus_tag="IWO45_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0334401.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2929..3792)
                     /locus_tag="IWO45_002986"
     CDS             complement(2929..3792)
                     /locus_tag="IWO45_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ0334402.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3794..4066)
                     /locus_tag="IWO45_002987"
     CDS             complement(3794..4066)
                     /locus_tag="IWO45_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0334403.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4083..4298)
                     /locus_tag="IWO45_002988"
     CDS             complement(4083..4298)
                     /locus_tag="IWO45_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0334404.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4390..>4619)
                     /locus_tag="IWO45_002989"
     CDS             complement(4390..>4619)
                     /locus_tag="IWO45_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAQ0334405.1"
                     /translation="TDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITE
                     HKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1417 a          782 c          850 g         1570 t
ORIGIN      
        1 tcctctagga aaaaattaac tagcaccacg ggttatcttt tatatagaag atatcgccgt
       61 atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg
      121 ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc
      181 ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc
      241 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
      301 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
      361 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
      421 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
      481 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
      541 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
      601 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
      661 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
      721 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
      781 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
      841 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
      901 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
      961 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     1021 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     1081 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     1141 aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa
     1201 aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt
     1261 ccatactctg atgttttata tcttttctaa aagttcgcta gataggggtc ccgagcgcct
     1321 acgaggaatt tgtatcgata agaaatagat ttaaaaattt cgctgttatt ttgtacattt
     1381 aacttgacgg tgacatctct ctattgtgag ttattagtgg tacagttttc aaccgtttta
     1441 attataaaaa agtggtgcat ttttaaattg gcacaaacag gtaacggtta ttgcaggtgt
     1501 atttcttatc tatgggttta acatggattt tatcattaaa atcatgagta ttgtccgaga
     1561 gtgattggtc ttgcgtatgg ttaaccctaa agttatggaa ataagactta gaagcaaact
     1621 taagagtgtg ttgatagtgc attatcttaa aattttgtat aataggaatt gaagttaaat
     1681 tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat tccaaatgcg
     1741 ttatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt acgcagataa
     1801 ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta aacagataac
     1861 taaaattaca aacaaatcgt ttaacttctg tatttgttta tagatgtaat cacttcagga
     1921 gtgataacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac
     1981 tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa
     2041 caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg
     2101 aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca
     2161 ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg
     2221 ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc
     2281 gtgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc
     2341 accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc
     2401 cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc
     2461 ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg
     2521 tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg
     2581 ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt
     2641 atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt tggaaagtta
     2701 cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta ataactacca
     2761 attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat ctatttctta
     2821 tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt tttataaaaa
     2881 aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt aaatacctgg
     2941 aagtttaggt gttttgggta ttggtggtgt aggtggctgc aagctttcca atttttgttg
     3001 aattgcttta aattcaggtg tctcttggtg tttatttaga accatttttt gctctattcg
     3061 ttctaaagtc ggttgaattt ctttccctga tactttacga ttcaattctt tttctaaggt
     3121 ttcttttgga ctaatagaag gtgtttccaa acttgaatag agttttactc cttctctgtt
     3181 atatagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat tggtcggtaa
     3241 gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctggctgtca ttggatcatc
     3301 tgcatacatg gtttcatatc gttcaattgt tcctaaatat gagttgattt taggtactgc
     3361 catgacatac atttttgttt cataaccttt ggcttgaagc attgttgcgg tttgaatagg
     3421 aacgtctgtt gttcgtcctg taccttcgat caccaaatta tacccttgat cgctcaaacg
     3481 gcttatgatc gcttctgtca tgcgattaga ataaggggta acgtgtttta ctacgtcttt
     3541 ttcataaagt ttcactagtt catcaaaatt agggtgctgt tgtttaaagg tatcattatc
     3601 aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac tggttttccc
     3661 tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgctt tttttccttg
     3721 aatcaattct tctaaattat catttaagcg attctcaaat tgtttgtcag taaaattgac
     3781 tatatttgcc atattaagcc actttctctt tattcaaaac ttgttttgag taacgattca
     3841 ttgaccgcca atactcatga acggcttgta attcttctaa agaataatca aaaagattta
     3901 cacgttttgc aagctttaat tggtttaata aattgacttc caataattga gagtcatttt
     3961 tatttaattc atggttcaaa acatagttta atactcgatt ataaacgctt gccgataatt
     4021 cgttaatgat ctctatttca aatgtttttt catacgtaac tgccatttca ttttcactcc
     4081 ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg atccataact
     4141 tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg caagtcagca
     4201 cgaacacgaa ccgtcttatc tcccattata tctttttttg cactgattgg tgtatcattt
     4261 cgttttgctt tttgtgcgcc taaatttccc acaatcactc acttctttct atttcttctt
     4321 actcttattt tatcatcaac aatcacaaat cacaagtgat ttgtgattaa tcacttgtga
     4381 tttgtgattc tattcttttt cgttttctaa ttgaataatt cgctcaagca tttcaaaaaa
     4441 tacgttctta tacatggata aaactttttt gtcatagcct ttatgctctg taatgccgtt
     4501 tttagaccag gtacttactt tattacttcg cttgataata ttttggaaaa ccaagttatc
     4561 ttctttatgt tgcttataca gttcttccag gtttgatttt atcgttgcgc tgtccgtat
//
LOCUS       DACEDD010000124         4430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEDD010000124 DACEDD010000000
VERSION     DACEDD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.127"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWO45_002990"
     CDS             complement(543..2048)
                     /locus_tag="IWO45_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0334406.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWO45_002991"
     CDS             complement(2045..2731)
                     /locus_tag="IWO45_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0334407.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO45_002992"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO45_002992"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0334408.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1543 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DACEDD010000125         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEDD010000125 DACEDD010000000
VERSION     DACEDD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.128"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWO45_002993"
     CDS             690..1658
                     /locus_tag="IWO45_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0334409.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWO45_002994"
     CDS             1669..2484
                     /locus_tag="IWO45_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0334410.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWO45_002995"
     CDS             2552..3262
                     /locus_tag="IWO45_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0334411.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWO45_002996"
     CDS             3298..>4425
                     /locus_tag="IWO45_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0334412.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACEDD010000126         4416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEDD010000126 DACEDD010000000
VERSION     DACEDD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.129"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            59..913
                     /locus_tag="IWO45_002997"
     CDS             59..913
                     /locus_tag="IWO45_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0334413.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="IWO45_002998"
     CDS             1010..3259
                     /locus_tag="IWO45_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0334414.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="IWO45_002999"
     CDS             3262..>4416
                     /locus_tag="IWO45_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0334415.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DACEDD010000127         4392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEDD010000127 DACEDD010000000
VERSION     DACEDD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.130"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            546..659
                     /locus_tag="IWO45_003000"
     CDS             546..659
                     /locus_tag="IWO45_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAQ0334416.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            complement(747..1040)
                     /locus_tag="IWO45_003001"
     CDS             complement(747..1040)
                     /locus_tag="IWO45_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0334417.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1443..2621)
                     /locus_tag="IWO45_003002"
     CDS             complement(1443..2621)
                     /locus_tag="IWO45_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0334418.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2725..3039)
                     /locus_tag="IWO45_003003"
     CDS             complement(2725..3039)
                     /locus_tag="IWO45_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334419.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3146..3361
                     /locus_tag="IWO45_003004"
     CDS             3146..3361
                     /locus_tag="IWO45_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334420.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3661..3903)
                     /locus_tag="IWO45_003005"
     CDS             complement(3661..3903)
                     /locus_tag="IWO45_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334421.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3890..4318)
                     /locus_tag="IWO45_003006"
     CDS             complement(3890..4318)
                     /locus_tag="IWO45_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0334422.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1388 a          834 c          654 g         1516 t
ORIGIN      
        1 ttttctcttt ttattctaag gttattttag tcttgtcctt taatacaaag attttatttt
       61 taaataaaaa atactctttt tttacgatta ttgaaaaaaa aagataaaac ttgattaatt
      121 gaagtattgc ttaaatttgg aagctatgag ggaagaataa aagagttagg acgggattat
      181 ctactatttt tctttgtttt aaatatttgt tatctttgaa cataaaagag aaatgtgtcg
      241 actgcagtgt tgtgtccctc atcacgaata aacggtgttc aactagctga tcttgtattc
      301 acttaaaaaa tggtaaagta cgagttgcta ttccccattt tcaattttct aacgctcagg
      361 acaaaatact gttttcacaa cctcattgga ataaaagcat atatattgtt tcaagaacga
      421 aatcttgtta tctttttaaa gggcatctgt ccaaaatcta attttaggag gtacgttttg
      481 gtatttattc gtacaaataa aagagaatta tctttattca tgtaacgcat ccaattgatt
      541 ttttcatgta cgctcctgga acgattttac tgacgattgc ttatttaact gcgaatcgct
      601 cattagcttt tatcatagct atccatattt tgaataacgt tcttggcttt gtattataga
      661 tacagatgac aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat acttgaactt
      721 ttttcaatga gtataaatta aaacttctaa ttttcaattc ccatcatctg atatgcccga
      781 ccgtctgaac ttcttttgaa aaagccaaat gtgattagat aacgtctttg ttcgacataa
      841 tcgccactag aaaatacgtt cagcagtatc gaattaacct ctgtttctgt ataagtcact
      901 ccatggtcaa agcgtttcaa gacttctttc aataggatgt atttcttttt tgttgaactt
      961 ggtacttgtt ttacttttcc atcattcatg aatgtgtcta aaactttcat cttttcttgc
     1021 tccgtataag tataaaccat cttctttctc cttttgattc taagaatact ccatttatgt
     1081 tagaatattc tggatttttt attaaagcgg ttactcttaa gaaagatatt atcatagtgc
     1141 tctttttttt actactactt ataggtagat ttattctaat ccattattct cccctaccaa
     1201 taattaaaga aaaaactgga tcagggtgtt caaattacat gctaagaaaa aagaaccctt
     1261 gaataccaag ggttctttct cagtctgata acaatgatta acgacggatt tctttgatac
     1321 ttgtgaaaag tcgttaaagt attgatataa aaggattttt attacctctg actacatatt
     1381 gactaatttg ttaaaaaatc gtttttcaag aatcctgtaa taccagtgct ttcaccgttt
     1441 ttttaactct ctaaagaact aaaagcaatc agaggtttta ccttttcatt tgacgttaag
     1501 tggcgataaa ttttcgaagt tgtttggtca gtatggccta agcgatcttt caagacttcc
     1561 cagctacagt gttccgctac taagcttgcc attgtatgac gagctaaatg agtatgaaat
     1621 ggcttattaa ttcctgagcg attcacgcac cgtttgacgt gatcatttac atactctcga
     1681 cgataaggaa caccacgttt actagaatca agatacacaa acaaatatgt ttcttctata
     1741 ataaacctgt aatgttgcct catctcaccc aaagccttaa tcaactgttg tagcttttcc
     1801 ataactagag gagttactag aactcgtcta gcagaactaa ctgtcttagt agtttcaatt
     1861 cgaaaatcct tactcttacc tttaactgcc gtttgcttat agaacagaat ttcatttctt
     1921 ttgaaatcta tatctccagg ctgtagagca agcgcctcgc tgactcggca gccagtgtaa
     1981 aatagaacat ggaaaagtac agacatcggt aattcctcat gaacaatgcc ataattcaaa
     2041 aaagttctca attcatcgac agtaagaaac ttgtcctcaa tattatttat ggcttctttt
     2101 aaatcagtta ccgttaactg atattttggc attctcacag acttcattgg attttcactt
     2161 atcaaattct ttctttgaca atatttaaaa atcatattta gaacttgaat aagtgattgt
     2221 aaactatttt tggcatagcc aacatttgtt ccattaacgc cacattcctt taaggtaaaa
     2281 agcaattctt gaaggcactc tggtgtcaaa tcagacaact ttttatcacc aatccatcga
     2341 acaataattt ttaattgttc ctctcgctta tcataggtta tttccttttg ttcatttaac
     2401 gaacggtaat attggaacca ttgctcacct acaacactaa acttgtcatt gcttgcaaca
     2461 atttttttct taagcctttg ttgtttctcc atttccagat aagcgagttg cgcctcacgt
     2521 tttgaattaa agccacgtct tgttgttcga tatgcttgaa agtaccatgt gatcctacca
     2581 gtagaactga tttgcttctt gaatcgatct gttttttcca tttattgatt cctcctattt
     2641 tgaaggaacc caacaagcag aaaccctttg gaaatctttc tatctatgtc atcattgtac
     2701 cgcatcttag aaagaaaatg taacttataa agatcgaagt agtcgcaatt tttctcgata
     2761 gtcattcgaa tcactctttc tcatccattc taaaacatca tcacgaaaat agtgtcctcg
     2821 aatcccgtac caacagggag gaaactcacc aagttttact aaacggttca gtgtactatc
     2881 acttatctga aatagtcgct ttaaatcagt atttgttaat accagaggta aatgactgac
     2941 tgcaagcaat ttttccgtgt aatcttccag ttttttttgt aaaattgaat agaactctat
     3001 ttccagagaa ttaacatttg ttttaacttc caactccatc acccttctct ctcaaaatca
     3061 aaacgaccac cacggtaccc cttaaaattg taaaaaaaca ctccagtact ccatcaaaac
     3121 ggactagaaa agaggaattt ttaatatgaa acaaggaatt gcagacataa aaataattaa
     3181 agaaattctt gaaaaatcta cggctaatgc aatagcattc ggaacaggaa tcaacttgag
     3241 tacagtaaaa aaacttaaaa gtggagagag agctgaagaa aaattaaatc ttgccgatgc
     3301 tattaaaatc acagaatttg gcatgaaaaa tatgccaacc aaaatcgaaa tatggaaata
     3361 gtagtttttc tgatctatca gctgttttgc caaagcacat aggaatctca cctcccccca
     3421 gtaatggcgg gccgatttgg ttacggaagt atcattatcg tagtcgcgat tctcatatta
     3481 aaatttgtcg cttaaaagct aaccttgtca tggcgtaact agagaatcaa aatctacatg
     3541 tactgataga tttgctattc atttgtcaaa gagcgctagg ctattttcag cctatggaaa
     3601 tcctttattt tgacccgata aaaaacttcc atagaatgaa aaggaaaggg aaaaaatact
     3661 ttaacgagtt gtatcaaatt caagtatctt ctcaatcaat tttgtttcca gttgtctacg
     3721 catatattca tctacataca taaccattcc tttttcttct tcataaactg gtttcatggc
     3781 taaccgtgaa atataatttt gatattttga cagaacgatg gacatggcaa ttgaatcacc
     3841 agaagctgcg gctttgattg tcgttagtgg tatgaatggt atatatcgtt tattttgctt
     3901 catatttgac acccaaatac tttcctagct cttccaaagc catttgtcgt tggtaccaaa
     3961 cactgccgag acttttccca ataatatcgc caatttcacg atcattaaga ccgatgaaga
     4021 aggacataaa aatgatttct tgtctttgac taggtaatgc tgcaatcgca tttgccaact
     4081 cctcattgga aataaagatt ttcataccaa ggacaataaa agtaccacta ctattcaaat
     4141 ttggttccaa ataatagaac tctgtaccta accctttttg ttccaatatt tccattgata
     4201 tttgccgttg gcgaaatcta gcgtacttct tttgaatatt taacgcctca ttgcagacta
     4261 ctttcttaca aaaactgtca aacgtatgtt gaatgtgggt ttcctctgag tcgcccataa
     4321 tattctcccc ctcaaaaaat tcattcaact cgttttactt ctatcccttt ccattattac
     4381 tacaatctag ga
//
LOCUS       DACEDD010000128         4332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEDD010000128 DACEDD010000000
VERSION     DACEDD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.131"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            63..296
                     /locus_tag="IWO45_003007"
     CDS             63..296
                     /locus_tag="IWO45_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334423.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="IWO45_003008"
     CDS             complement(455..577)
                     /locus_tag="IWO45_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0334424.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            767..991
                     /locus_tag="IWO45_003009"
     CDS             767..991
                     /locus_tag="IWO45_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334425.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1434..1640
                     /locus_tag="IWO45_003010"
     CDS             1434..1640
                     /locus_tag="IWO45_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0334426.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="IWO45_003011"
     CDS             1640..1891
                     /locus_tag="IWO45_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334427.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            2072..2344
                     /locus_tag="IWO45_003012"
     CDS             2072..2344
                     /locus_tag="IWO45_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334428.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2789..2968
                     /locus_tag="IWO45_003013"
     CDS             2789..2968
                     /locus_tag="IWO45_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334429.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     TAQEKAGFDVPALKK"
     gene            3027..3383
                     /locus_tag="IWO45_003014"
     CDS             3027..3383
                     /locus_tag="IWO45_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334430.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1526 a          676 c          836 g         1294 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg accgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     3181 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     3241 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     3301 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     3361 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     3421 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     3481 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     3541 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     3601 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     3661 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     3721 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     3781 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     3841 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     3901 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     3961 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     4021 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     4081 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     4141 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     4201 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     4261 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     4321 taaaggtttt ta
//
LOCUS       DACEDD010000129         4317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEDD010000129 DACEDD010000000
VERSION     DACEDD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.132"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IWO45_003015"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IWO45_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0334431.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="IWO45_003016"
     CDS             complement(734..1573)
                     /locus_tag="IWO45_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0334432.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..2645
                     /locus_tag="IWO45_003017"
     CDS             1758..2645
                     /locus_tag="IWO45_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0334433.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3239..3934)
                     /locus_tag="IWO45_003018"
     CDS             complement(3239..3934)
                     /locus_tag="IWO45_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0334434.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(3918..4316)
                     /locus_tag="IWO45_003019"
     CDS             complement(3918..4316)
                     /locus_tag="IWO45_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0334435.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1428 a          853 c          642 g         1394 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccgattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaagg agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     2521 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     2581 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     2641 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     2701 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     2761 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
     2821 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
     2881 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
     2941 ttttaaaaaa gactaaaaat atcccttctg gacttgttct tcttattctg ttatactaga
     3001 gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
     3061 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
     3121 gagaaataag taatagttag taatagtcac ccaaacttta aattaatgaa tattgaatcg
     3181 tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc
     3241 agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc
     3301 aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta
     3361 gtagctcttt acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt
     3421 cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag
     3481 tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat
     3541 aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa
     3601 tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg
     3661 cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag
     3721 catcctctat catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc
     3781 cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca
     3841 gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac
     3901 tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct
     3961 tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac
     4021 attgttcctg gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat
     4081 tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca
     4141 aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa
     4201 caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc
     4261 ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatc
//
LOCUS       DACEDD010000130         4282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEDD010000130 DACEDD010000000
VERSION     DACEDD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.133"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IWO45_003020"
     CDS             complement(37..1257)
                     /locus_tag="IWO45_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0334436.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IWO45_003021"
     CDS             complement(1277..1882)
                     /locus_tag="IWO45_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0334437.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IWO45_003022"
     CDS             complement(1931..2863)
                     /locus_tag="IWO45_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0334438.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IWO45_003023"
     CDS             complement(3284..4144)
                     /locus_tag="IWO45_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0334439.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DACEDD010000131         4175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEDD010000131 DACEDD010000000
VERSION     DACEDD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.134"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            149..1096
                     /locus_tag="IWO45_003024"
     CDS             149..1096
                     /locus_tag="IWO45_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0334440.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1108..2028
                     /locus_tag="IWO45_003025"
     CDS             1108..2028
                     /locus_tag="IWO45_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0334441.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2069..3526
                     /locus_tag="IWO45_003026"
     CDS             2069..3526
                     /locus_tag="IWO45_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0334442.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3871..>4175
                     /locus_tag="IWO45_003027"
     CDS             3871..>4175
                     /locus_tag="IWO45_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0334443.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLI"
BASE COUNT         1315 a          712 c          834 g         1314 t
ORIGIN      
        1 tcccgttttt ttctctaata ctcaggccaa aacgctgatt tcacaacctc tcctatactt
       61 tgtttcattt tccctatact ttgtttattt attctcatta ttgaaagcgc tataattacg
      121 ttgcgaatga atcgaaaggg gtttttagat gaaaaaacga aaggggttca atcatttttt
      181 tatgaatcta tggcgttata aagcacttgt cctgatggct gtaccgggta tgctttggat
      241 gatttttttc ttttacatac ctgtaatggc gaatgtcgta gcattcaaaa attttcatat
      301 ttcagcagat gggttcttgg ctagcttgag ggaaagtcct tgggtgggtt gggaaaattt
      361 caaattttta ttttcttcgg atcaagcatt tctcatcact aaaaatacaa ttttatataa
      421 tgtagcattt atccttttga atctgctgat ctcagtcgta tttgcaatca ttatgagcga
      481 gttgagaaat aaacgactcg ttaaagttta tcaaacgatg tccttactac cttattttct
      541 atcttgggtc attatcagtt actttgttta cgctttttta agtcctgaca aaggaatatt
      601 caatcagtgg atcactggac atggaggaga agctgtcaat tggtataatg aaccgaaata
      661 ttggccgttt atcttggtgt tcattggtac atggaaaggg attggttata acagcattat
      721 ttactttgca tctgttatgg gaatcgatcc gacttattat gaagctgcta tggttgatgg
      781 agctagcaaa tggcagcaga tcaaacatgt gacgatacca caacttattc cactaatgac
      841 gattttaacg attttagccg taggaaacat tttccgagct gattttggcc tgttttataa
      901 cattcctagg aattccggtg cattatatga tgtgactcaa gttttggata cgtatatcta
      961 caatggcctg acatccacag gagattttgg tatgacggct gctgcaggac tttaccaatc
     1021 agttgtcggc tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt ttgatagcga
     1081 ttctgcatta ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc
     1141 cgctctttca ataagacggc taattttttc tctaatataa tgattgcact ctttgctatt
     1201 tcctgtattt tgccatttat atttgttatc gccatctctt ttaccgatga gtccatattg
     1261 tcgactcacg ggtacagttt ttggccagaa aagttcagta tatttggtta tacgtacttg
     1321 ttcgaccaga tgcaggataa aattttccaa gcgcttttcg tcactgtact tgttactgtt
     1381 ttaggtacac tgatcaatag cacggctact tctttatatg cttatgcgat ttcacggtca
     1441 aactttccat tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt gttctcacca
     1501 gggatggtgg ccaattatct tgttatgaca aatcttcttc agttaaaaga tacgatttgg
     1561 gcattgatct tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt
     1621 aaacgttcgg tctctgattc gattattgaa tcggcacgga tcgatggagc tagcgaactg
     1681 cgaatatttg ttcagatcgt tttaccgtta gctgttccag gtattgcgac aatcagttta
     1741 tttgccgcat tgggttactg gaatgactgg ttcaatgctt tactatacat ccaagatgat
     1801 aagctagttc cactacagta tcttttgatg aaaatccaaa gtaatattca atatctgaca
     1861 caaaattcag gagcaggaag ccaattgaca ggcggattag cctcagttcc aggagaatct
     1921 gccagaatgg cgatcgtagt catctcgacg ttgcctattg cgatcagcta tccatttttc
     1981 cagaagcatt ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag aaacaaaaat
     2041 ataaatcata aaattaggag gaagaaaaat gaagttatgg aagaaactag cctttacagg
     2101 tgtttcagcg ttgttgttgg gaacattagc agcatgcgga ggttctggtt ctaaagacca
     2161 ggcagaagct agcaattcgg acactttgca gatgtatcaa atcggagata aaccagataa
     2221 tttcgatcaa ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt
     2281 caatatcaat tacattggct ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc
     2341 cggggaaaat tatgatatcg cctttgcgaa caactatgtt tccaatgccc aaaaaggagc
     2401 ctttgctgat ttgactgaat tagcgcctaa gtacgctgaa aaagcttata atgatttaga
     2461 tgaagcatat attaaaggaa atctggtcaa tggaaaatta tatgctttcc cagtcaatgg
     2521 aaatgtcttt gctcaacaag tacttacttt taacaaagct ttactagata aatacgatct
     2581 ttcaattgat ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg aattccatga
     2641 aaaagaacca aacacggcag cttttgccat tggacaaggc ttcaaagtcc aagggaactt
     2701 tgattatcct ttagggaata cgttaccatt tgctattgat ctggatggag atgattcgaa
     2761 aatcatcaac caatacgata atgaaacgta tatcggactg ttacgtacaa tgcatagctg
     2821 gtacaaacaa ggactcattc caagtgacgc tgcaacgagc aataaggatt atccactaga
     2881 aggaaatact tggctaatgc gtggagaaac acaaggaccg tatgattatg gcgatacaat
     2941 tttgacaaat gcagcaaaac aagaacttgt ctcaaaagca attactgttc cactaaaatc
     3001 tacagctcaa gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa
     3061 atcagtagaa cttctagggc tgcttaacag tgatccagaa ttattgaatg ggcttgtttg
     3121 ggggatcgag ggagaagcat gggaaaaagt cgatggttca gatgataaaa tcaaattgct
     3181 agatggttat cagccaaata cacatatgtc tgcttggaat acaggaaaca acaagattct
     3241 atatacacaa gagtctatta cggatgacat gattgccaaa cgtgatcaat cgattgctga
     3301 tgcagaaact tcaccgattt taggattcag cttcaacact gacagtgtca aaacagaatt
     3361 aagtaatatt tctaatgtca tgaatcaata tttagatgga ttgaatacag ggactgttga
     3421 tccagatgaa acattaccga aattgaaaga cgctttggac aaggctggct atgataaggt
     3481 attgaaagaa atgcaaaaac aatatgatga gttccgacaa gaataataat gaataaagac
     3541 aaaggggttg tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa
     3601 gccagtttct cggtattaag ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa
     3661 tttttcttac tactcgaaac taagcggcta tttcacaaac tcttggtaaa cggtgttcaa
     3721 tcagctaacc ctcggtattc gctcacaaaa cgacaaaata tgaggaacta tttttccgtt
     3781 tgtttctcta ataatcaggt caaggtgctg attgcccaac ctttttttgt ataatgagaa
     3841 agtataaact taatgtttgg aggagaagat atgttaggga aggttttgga agagttattt
     3901 atccggatct gggtcattat aaaactaacc ttgtatttct ggatatatac atttgccggc
     3961 gggatcatct ttggattagg tgcggcatgg aaaacagtca atgaactatt ttatttgtac
     4021 ggattcgagt ataaagaaat cacaataaaa agaggctgga atatatataa aagaaatttt
     4081 ttaagaggca atctgctatt tagtttattt ttatctggta ctgctttatt gagttacaat
     4141 ttatatttat ctgttcaaat caaagggctc atttt
//
LOCUS       DACEDD010000132         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEDD010000132 DACEDD010000000
VERSION     DACEDD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.135"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWO45_003028"
     CDS             179..775
                     /locus_tag="IWO45_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0334444.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWO45_003029"
     CDS             899..2698
                     /locus_tag="IWO45_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0334445.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWO45_003030"
     CDS             complement(2976..3188)
                     /locus_tag="IWO45_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334446.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1188 a          689 c          712 g         1452 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DACEDD010000133         4034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEDD010000133 DACEDD010000000
VERSION     DACEDD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.136"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..234
                     /locus_tag="IWO45_003031"
                     /pseudo
     CDS             79..234
                     /locus_tag="IWO45_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..1530
                     /locus_tag="IWO45_003032"
     CDS             253..1530
                     /locus_tag="IWO45_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0334447.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="IWO45_003033"
     CDS             1540..2196
                     /locus_tag="IWO45_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0334448.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="IWO45_003034"
     CDS             2196..3572
                     /locus_tag="IWO45_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0334449.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="IWO45_003035"
     CDS             3702..>4034
                     /locus_tag="IWO45_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0334450.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DACEDD010000134         3944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEDD010000134 DACEDD010000000
VERSION     DACEDD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.137"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1211)
                     /locus_tag="IWO45_003036"
     CDS             complement(<1..1211)
                     /locus_tag="IWO45_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0334451.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..2796)
                     /locus_tag="IWO45_003037"
     CDS             complement(1201..2796)
                     /locus_tag="IWO45_003037"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0334452.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            2921..3706
                     /locus_tag="IWO45_003038"
     CDS             2921..3706
                     /locus_tag="IWO45_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAQ0334453.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            3699..3941
                     /locus_tag="IWO45_003039"
     CDS             3699..3941
                     /locus_tag="IWO45_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334454.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1233 a          762 c          640 g         1309 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtga
     2521 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
     2581 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
     2641 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     2701 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     2761 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
     2821 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
     2881 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
     2941 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
     3001 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
     3061 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
     3121 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
     3181 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
     3241 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
     3301 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
     3361 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
     3421 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
     3481 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     3541 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     3601 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     3661 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     3721 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     3781 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     3841 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     3901 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DACEDD010000135         3865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEDD010000135 DACEDD010000000
VERSION     DACEDD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.138"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(248..469)
                     /locus_tag="IWO45_003040"
     CDS             complement(248..469)
                     /locus_tag="IWO45_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334455.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="IWO45_003041"
     CDS             complement(567..812)
                     /locus_tag="IWO45_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334456.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="IWO45_003042"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="IWO45_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="IWO45_003043"
     CDS             complement(2036..3037)
                     /locus_tag="IWO45_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ0334457.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="IWO45_003044"
     CDS             complement(3196..3462)
                     /locus_tag="IWO45_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0334458.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="IWO45_003045"
     CDS             complement(3452..3808)
                     /locus_tag="IWO45_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0334459.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1223 a          698 c          511 g         1433 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatatatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cgaat
//
LOCUS       DACEDD010000136         3600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEDD010000136 DACEDD010000000
VERSION     DACEDD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.139"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..265
                     /locus_tag="IWO45_003046"
     CDS             <1..265
                     /locus_tag="IWO45_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334460.1"
                     /translation="ANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHT
                     ISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            410..715
                     /locus_tag="IWO45_003047"
     CDS             410..715
                     /locus_tag="IWO45_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334461.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1332..>3600
                     /gene="esp"
                     /locus_tag="IWO45_003048"
     CDS             1332..>3600
                     /gene="esp"
                     /locus_tag="IWO45_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0334462.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1314 a          498 c          687 g         1101 t
ORIGIN      
        1 agctaattat ttttctgtaa gtgaaactaa attaagagaa agctttaaga atgaaatgca
       61 tattacgatt tataattata tctctaagcg aaaaatttct acggctaaaa taatgttgaa
      121 atcaaaccat actatcagtg aagtttcttt aggtttagga ttttcagatt cttcacactt
      181 ttcgagagta ttcaaaaagt acgcaggtgt atctcctaag caatatcaat taggtttagt
      241 ggataatttc aacaacatta gctgagaggt tttattttat tatgagacta tatcatttac
      301 gtagtttgga ggagttgagg aaattggatt ttaacaaaaa attttttggg aaatttctga
      361 tttgagttgt ttcgtagaat ctgagcgtct aagtaatggt aataatagtg tgttaaatga
      421 atatgacaaa gtagtaatta ataaagtaaa aagtttatgt gaagaagcta gagaatctat
      481 cagtcatatt aatggtagca attttaagca aacattattt aatctgatta agctagatgc
      541 taaaatatca agttatttat tctttttaat acatgatgaa tttatggatt atcaaaaatt
      601 ggatcagtta attgaaagag aaagctggga agattattac gtgggtctaa ctttttcaga
      661 aaagttaaac aactacaagt taatagatta taagtatttg agtgaaagca attaattatt
      721 catgtagagt actagacact ttaaatatgg acaattcaat tataaagaaa aagaatcaat
      781 aatttaatga ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg
      841 ttgataactc caatcattcg tttaagattc taggaatcat tcaggaatgc tgtgaaaccg
      901 atactttata attataaaga aggtgatttc aaattccgaa gaaggaattt gaagtcacct
      961 ttttacttat gtcacagtct cattcagtaa tgcgggataa ggctccttat tccgctattt
     1021 gaatactctg aagtttaatc taagaaagag taatattttg aaaacatgct tcatcgcata
     1081 gaaatgaact taatttcaga aagaatacat acatatcttt ttatatgttt tcagctgctg
     1141 aatggttaaa aagcacaaat atccgaaaaa aacatgaaga aatgtacttt ttttgttgta
     1201 atgtgcatga aattctttaa gtaaactagt taacataagt ttagtaagat tcctattctg
     1261 tgaataatac gtatcagatt tatcttttat tcttactaag aaaataacta ttaagggagt
     1321 tgatttgaat aatggttagc aagaataata agagagtatt tctagaaaaa acaaagaaac
     1381 gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt ttggttggtg
     1441 ttggtcttgt cttgggaaca actgaactag ttcaagcaca agatgaaatt tctccaagca
     1501 ccccacttga aacagcgata agctccgtac aagtaggtga caaagtagct tcggggaata
     1561 cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta caatttagtc
     1621 ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa gtttacggaa
     1681 aacataatat tgcagcatca actaaaaact gggaaatccg tcttcaatta gatgaacgat
     1741 tagctaaata tgtagaaaaa attcaggtcg atccaaagaa aggaattgga tcttctagac
     1801 ggacctttgt aagaattaat gattcgctgg gtagacctac aaatatttgg aaggtcaatt
     1861 atattcgtgc aagtgatgga ttatttgctg gggctgaaac aactgataca caaactgctc
     1921 ctaatggggt aattacgttt gaaaagagct tagatgaaat ttttaaagag ataggcatag
     1981 acaatctcaa gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat caagacgatg
     2041 ataaaattgt accaggtatt gatagtacgg gctacttttt aacagattca gatgatttct
     2101 ataatagctt agatgtatct gaaaataatc ctgaccagtt taaacatgga tcagtaagtg
     2161 caaaatatga ggaacctaat actcaaacaa aagatggttc aggatccaca ggtgctaatg
     2221 gagctataat cttagatcat aagttaacga aaaattataa tttctcatat tcagcttccg
     2281 caaagggcac gccttggtat gctaattaca aaattgatga acgattagtt ccatatgtag
     2341 cgggtataca gatgcacatg gttcaggctg acaaagtaac atatgatgtt tcttttgaat
     2401 cagggaaaaa agtggctgat ttagcaattg aacggcgcaa agatcatgaa aattatggtg
     2461 taggttcaat tactgataat gatcttacca aacttattga ttttgctaat gcaagtccac
     2521 gtccagtcgt tattcgatat gttttacaat tgactaagcc attagatgaa atcttagaag
     2581 atatgaaagc aacagcacaa gttgaagaaa ataaaccatt tggtgaagat ttcatctttg
     2641 attcttggtt gtcggatacg aataaaaaat taatccagaa cacttatgga acaggttatt
     2701 attatttgca agatattgat ggtgatggaa accctgacga taaagaagag agcggagaca
     2761 cgaatccata tatcgggaaa cctgaattag aagaagtata tgatgttgac acaacagtta
     2821 aggggaaagt attcatccac gagttagcgg gaacaggtca caaagcccaa cttgttgata
     2881 aagaaggtac tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa
     2941 tttcagatac tgtagaattt gaatttacgg gtgtagattc aagtaaatta atcgcgaaag
     3001 atgaattaaa aatccaaatc gtttctccag gttttgataa accagaagaa ggttcaaccg
     3061 ttattaagga atcaccaaaa gcggttgata aacaaaccgt ggtagttgga tttaaaccag
     3121 atgctaaaga atcaattcgg aataataaaa acttacctga agatgcagag tattcatgga
     3181 aaacagagcc tgatacttct aacgttactg atagtacgaa aggtattgta actgttaaga
     3241 tcggaaatcg aactttcgac gtggatgtag agtttgctgt aaaagcttct caagctatgg
     3301 aaaatgatgc aacatacgta cctataacaa caactccaga aacgacagtt caaagtggaa
     3361 aacctacatt tgataaacca gatgttcctt tagctaaaga tgccttttca attttagatg
     3421 tctataataa ggacttcggt aatgcaagtg ttgatgcaaa tactggtatt gttacattca
     3481 ctccagctaa aggtgtagga gaatcggagc cgattactgg aacaattcct attaaaattg
     3541 tttaccaaga tggttctgta ggcacgaccg atttagcagt aactgtaagt aaagatattt
//
LOCUS       DACEDD010000137         3398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEDD010000137 DACEDD010000000
VERSION     DACEDD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.141"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..276
                     /locus_tag="IWO45_003049"
     CDS             <1..276
                     /locus_tag="IWO45_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ0334463.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IWO45_003050"
     CDS             419..640
                     /locus_tag="IWO45_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0334464.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IWO45_003051"
     CDS             656..1498
                     /locus_tag="IWO45_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334465.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IWO45_003052"
     CDS             1561..3363
                     /locus_tag="IWO45_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ0334466.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1229 a          469 c          702 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DACEDD010000138         3330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEDD010000138 DACEDD010000000
VERSION     DACEDD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.142"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            12..3161
                     /locus_tag="IWO45_003053"
     CDS             12..3161
                     /locus_tag="IWO45_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0334467.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1165 a          559 c          711 g          895 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagttga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa
     1321 tgtttccgaa atccacttgg tttggcttta caggtacacc gattttccct gagaatcgga
     1381 aacaagccaa agggcaattg gcacgtacga cccatgatca gtatggagag gtcttgcaca
     1441 cctataccat caagaatgca ttagaagatg gctcggtttt aggatttcaa gtagagcatg
     1501 aaaacaccgt ggagccgact tcattagaga ataaaatcta tcggaaatta aaagaagtag
     1561 aaacatatgc agagtattca ccggaacaga tcaatcgtat gatcgatcaa atggagtctg
     1621 tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat atccaaaaag
     1681 tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt cgaaatggcc
     1741 gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa aagtattatc
     1801 gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt cccaatcagc
     1861 ctatccgtga aggacgaacg atggaggatc ctgatttccc acgaatcgct attacctatt
     1921 cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa aaaattattg
     1981 aggaatataa tcagtattat gatactgcct ggtcactagc agacattgag cgttataacg
     2041 gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa
     2101 tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcccct acgattcaaa
     2161 ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagccttt tcacgtacga
     2221 accgtacgta tcctgaaaaa acaaaaggac taattgttac tttccgaaag ccagcgacaa
     2281 tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agctaaagaa gaatcgggtt
     2341 tgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat ggtaagttaa
     2401 aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc cgtatcgagt
     2461 acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact tatgatgatt
     2521 acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt ttggaagaac
     2581 aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca gaaaaagagg
     2641 gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg tatgatattg
     2701 attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca gatgcacgag
     2761 aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa gaagtttacc
     2821 gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat atctttgtca
     2881 ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt tccaatgagt
     2941 ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca ggtgaagatc
     3001 caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat aaagccaaac
     3061 atccagaagc gaaaccattt aaatacccac aagaaatgaa gcgagcatgg cgtaaaatgt
     3121 tagatgatga gttgattcca cttgaaaatg aattgaggta aacgagaaga gttaagaaaa
     3181 ttgtacgatt caaagatagt agtaaagtgg aagtgtagaa aatagggata gatgtctttt
     3241 tacttgttca cattttataa cggagtattt aagaaagcta ttgaataatg attcttccat
     3301 ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DACEDD010000139         3274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEDD010000139 DACEDD010000000
VERSION     DACEDD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.143"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            177..473
                     /locus_tag="IWO45_003054"
     CDS             177..473
                     /locus_tag="IWO45_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334468.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            480..2432
                     /locus_tag="IWO45_003055"
     CDS             480..2432
                     /locus_tag="IWO45_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0334469.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="IWO45_003056"
     CDS             complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="IWO45_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0334470.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1156 a          538 c          566 g         1014 t
ORIGIN      
        1 aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg
       61 caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca
      121 caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc
      181 aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc
      241 aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag
      301 taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg
      361 atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc
      421 gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa
      481 tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga
      541 tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat
      601 ttgcaacaag gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg
      661 aatatcccat tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac
      721 agcacaaatt caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca
      781 aagataaagg tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc
      841 aaaactgtac agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta
      901 tagaaatcaa tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct
      961 cggaagaaga atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta
     1021 ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa
     1081 aaagagaaga aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta
     1141 agatggttgt tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg
     1201 gaacctacta tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat
     1261 atgagacaca accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga
     1321 tgcactacta cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt
     1381 ttgaagacct taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg
     1441 tgttccgttc tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata
     1501 caagaaaatt caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt
     1561 ggtcttatta tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc
     1621 acgaattagg gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc
     1681 cttatactac aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc
     1741 gaatgattag tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag
     1801 aattaaggtt gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa
     1861 aacaatgctt aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca
     1921 gaggattcaa aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa
     1981 caagtcatca attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg
     2041 ccgataaccc tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt
     2101 gtagaacgga tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta
     2161 atgaccattc gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa
     2221 gaccattaac aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga
     2281 agtatgctca atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta
     2341 aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat
     2401 ttgattggaa agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct
     2461 cttgtgcttt atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt
     2521 ttctttcata tacatgaaaa tagtagttat acggattttt atcattcttt atcccttttt
     2581 gggcaaatac ctcattccat tcctcataat tcacttctgg aaaaaaagta tctccttcga
     2641 attcatgatg tatttttgtg atgtacattt tctcaacata agggaaaaac aaattataaa
     2701 tctgttctcc tccgaaaata aaaatttctt cttcgttttt acataactca aaaacatctt
     2761 ctattgaatg aacaatttca caaccattaa aggtaaaccc cttatctctc gtcagaataa
     2821 tatttcttct gtcaggtaag gctcttccga ttgattcaag gttcttccta cctaatatta
     2881 tcggatgtcc ctttgtagta tttttaacat attcccagtc cttgggaatc ctccaaggaa
     2941 tgtcattctc tttgccaatc actctattct tatccatcgc agcaatcaaa gaaactttca
     3001 tttataacac agccttttta actaatttac catctaattt cttatttcta tcaaaaaacc
     3061 ttttctcatt atcctgcccg ttagttgaat aagcaatttt ttctataagt tatattatca
     3121 taataccaca aaatcacaaa ttttcattca attatcattt tcaattgcat tttataaatc
     3181 attttgtatg taatttggta tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca
     3241 ttttgtcatt ttgtactcca aaactgaact actt
//
LOCUS       DACEDD010000140         3092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEDD010000140 DACEDD010000000
VERSION     DACEDD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.144"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWO45_003057"
     CDS             complement(25..144)
                     /locus_tag="IWO45_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0334471.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWO45_003058"
     CDS             complement(159..1343)
                     /locus_tag="IWO45_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0334472.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="IWO45_003059"
     CDS             complement(1602..1892)
                     /locus_tag="IWO45_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334473.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="IWO45_003060"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="IWO45_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          844 a          739 c          540 g          969 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattattaa tt
//
LOCUS       DACEDD010000141         3032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEDD010000141 DACEDD010000000
VERSION     DACEDD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.145"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..955)
                     /gene="murB"
                     /locus_tag="IWO45_003061"
     CDS             complement(20..955)
                     /gene="murB"
                     /locus_tag="IWO45_003061"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0334474.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1028..1948)
                     /locus_tag="IWO45_003062"
     CDS             complement(1028..1948)
                     /locus_tag="IWO45_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0334475.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2061..2813
                     /gene="xth"
                     /locus_tag="IWO45_003063"
     CDS             2061..2813
                     /gene="xth"
                     /locus_tag="IWO45_003063"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0334476.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          854 a          611 c          526 g         1041 t
ORIGIN      
        1 ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat attgcttgtt
       61 tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct ttgattactt
      121 cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg atattgacga
      181 tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct tgcaatcctg
      241 cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca cttccgcatg
      301 aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc attcttgctt
      361 tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg atagcacgta
      421 attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc aaagttttca
      481 atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc tctccgccat
      541 acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg caagcaaatt
      601 caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt gaggcacctg
      661 cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc agcatgatga
      721 ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct aataccatcc
      781 aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct tgttttttcg
      841 ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc atcaatggtt
      901 catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta ttcactgagg
      961 attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac aatcttgcca
     1021 gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat aattcatgac
     1081 ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa cggatctgag
     1141 aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt gtccagtcgc
     1201 caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc agatcgttta
     1261 cttcataagt accattttta cttgttactt gatatttttc tatattagcg ccacttgcta
     1321 aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg gcatattcca
     1381 aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca tctaataaat
     1441 acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct gtcgtttctt
     1501 ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata cgtttcaatt
     1561 cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt tggttcttat
     1621 cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt ttgtcaatat
     1681 aaacatggat accattttct agacattttt tggctaattc ataatgtact tttgttgctg
     1741 cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt tcatatagat
     1801 gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt gttgcaaaat
     1861 aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt tctgcgatat
     1921 tccctacacc gattacacca atcttcatct actttcctcc tggatcattt agttactttc
     1981 atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta aggaaattaa
     2041 ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg cttcgtgcca
     2101 tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt ttctgtctgc
     2161 aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat tatcaatatt
     2221 ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa aaaccagcat
     2281 tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt ctgattactt
     2341 tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa agcgaattaa
     2401 aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg gaaaatctaa
     2461 aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa aagattgatt
     2521 tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa agagcggctc
     2581 tttctcgact gttggataac gggtttatcg atacattccg ttatttttat ccaacccagg
     2641 aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat gctggttggc
     2701 ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat gcaaagatcc
     2761 atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa taaagaggtt
     2821 gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     2881 tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt atcgagagat
     2941 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     3001 tttttcgttt ggttgggctt attaccgcag at
//
LOCUS       DACEDD010000142         2944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEDD010000142 DACEDD010000000
VERSION     DACEDD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.146"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..977
                     /locus_tag="IWO45_003064"
     CDS             6..977
                     /locus_tag="IWO45_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0334477.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="IWO45_003065"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="IWO45_003065"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0334478.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="IWO45_003066"
     CDS             2004..2612
                     /locus_tag="IWO45_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ0334479.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          780 a          624 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctc
//
LOCUS       DACEDD010000143         2826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEDD010000143 DACEDD010000000
VERSION     DACEDD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.149"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWO45_003067"
     CDS             7..624
                     /locus_tag="IWO45_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334480.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IWO45_003068"
     CDS             1644..2813
                     /locus_tag="IWO45_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0334481.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         1051 a          378 c          447 g          950 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacaga
//
LOCUS       DACEDD010000144         2751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEDD010000144 DACEDD010000000
VERSION     DACEDD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.150"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..1380
                     /gene="tet(L)"
                     /locus_tag="IWO45_003069"
     CDS             7..1380
                     /gene="tet(L)"
                     /locus_tag="IWO45_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0334482.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            1944..>2751
                     /locus_tag="IWO45_003070"
     CDS             1944..>2751
                     /locus_tag="IWO45_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAQ0334483.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERD"
BASE COUNT          880 a          408 c          570 g          893 t
ORIGIN      
        1 ttaattatga agtgtaatga atgtaacagg gttaaataca accaaatttt aatttggctt
       61 tgcgttttat ctttttttag cgtattaaat gaaatggttt tgaacgtctc attacctgat
      121 attgcaaatg attttaataa accacctgcg agtacaaact gggtgaacac agcctttatg
      181 ttaacctttt ccattggaac agctgtatat ggaaagctat ctgatcaatt aggcatcaaa
      241 aggttactcc tatttggaat tataataaat tgtttcgggt cggtaattgg gtttgttggc
      301 cattctttct tttccttact tattatggct cgttttattc aaggggctgg tgcagctgca
      361 tttccagcac tcgtaatggt tgtagttgcg cgctatattc caaaggaaaa taggggtaaa
      421 gcatttggtc ttattggatc gatagtagcc atgggagaag gagtcggtcc agcgattggt
      481 ggaatgatag cccattatat tcattggtcc tatcttctac tcattcctat gataacaatt
      541 atcactgttc cgtttcttat gaaattatta aagaaagaag taaggataaa aggtcatttt
      601 gatatcaaag gaattatact aatgtctgta ggcattgtat tttttatgtt gtttacaaca
      661 tcatatagca tttcttttct tatcgttagc gtgctgtcat tcctgatatt tgtaaaacat
      721 atcaggaaag taacagatcc ttttgttgat cccggattag ggaaaaatat accttttatg
      781 attggagttc tttgtggggg aattatattt ggaacagtag cagggtttgt ctctatggtt
      841 ccttatatga tgaaagatgt tcaccagcta agtactgccg aaatcggaag tgtaattatt
      901 ttccctggaa caatgagtgt cattattttc ggctacattg gtgggatact tgttgataga
      961 agaggtcctt tatacgtgtt aaacatcgga gttacatttc tttctgttag ctttttaact
     1021 gcttcctttc ttttagaaac aacatcatgg ttcatgacaa ttataatcgt atttgtttta
     1081 ggtgggcttt cgttcaccaa aacagttata tcaacaattg tttcaagtag cttgaaacag
     1141 caggaagctg gtgctggaat gagtttgctt aactttacca gctttttatc agagggaaca
     1201 ggtattgcaa ttgtaggtgg tttattatcc atacccttac ttgatcaaag gttgttacct
     1261 atggaagttg atcagtcaac ttatctgtat agtaatttgt tattactttt ttcaggaatc
     1321 attgtcatta gttggctggt taccttgaat gtatataaac attctcaaag ggatttctaa
     1381 atcgttaagg gatcaacttt gggagagagt tcaaaattga tccttttttt ataacaggaa
     1441 ttcaaatctt tttgttccat taaagggcgc gattgctgaa taaaagatac gagagacctc
     1501 tcttgtatct tttttatttt gagtggtttt gtccgttaca ctagaaaacc gaaagacaat
     1561 aaaaatttta ttcttgctga gtctggcttt cggtaagcta gacaaaacgg acaaaataaa
     1621 aattggcaag ggtttaaagg tggagatttt ttgagtgatc ttctcaaaaa atactacctg
     1681 tcccttgctg atttttaaac gagcacgaga gcaaaacccc cctttgctga ggtggcagag
     1741 ggcaggtttt tttgtttctt ttttctcgta aaaaaaagaa aggtcttaaa ggttttatgg
     1801 ttttggtcgg cactgccgac agcctcgcag agcacacact ttatgaatat aaagtatagt
     1861 gtgttatact ttacttggaa gtggttgccg gaaagagcga aaatgcctca catttgtgcc
     1921 acctaaaaag gagcgattta catatgagtt atgcagtttg tagaatgcaa aaagtgaaat
     1981 cagctggact aaaaggcatg caatttcata atcaaagaga gcgaaaaagt agaacgaatg
     2041 atgatattga ccatgagcga acacgtgaaa attatgattt gaaaaatgat aaaaatattg
     2101 attacaacga acgtgtcaaa gaaattattg aatcacaaaa aacaggtaca agaaaaacga
     2161 ggaaagatgc tgttcttgta aatgagttgc tagtaacatc tgaccgagat ttttttgagc
     2221 aactggatcc aggagaacaa aaacgatttt ttgaggaaag ttataaatta ttttccgaac
     2281 gatatggcaa gcaaaatatt gcttatgcaa cagttcataa tgatgagcaa acccctcaca
     2341 tgcatttagg tgttgtgcct atgcgtgatg gaaaactgca aggaaaaaat gtgtttaatc
     2401 gtcaagaact gttatggcta caagataaat tccccgagca tatgaaaaaa cagggttttg
     2461 agttgaagcg tggtgaacgt ggctctgacc gtaaacatat tgagacagct aaatttaaaa
     2521 aacaaacttt ggaaaaagag attgattttc tagaaaaaaa tttagcagtt aaaaaagatg
     2581 aatggactgc ttatagcgat aaagttaaat cagatttaga agtaccagcg aaacgacaca
     2641 tgaaaagtgt tgaagtgcca acgggtgaaa agtccatgtt tggtttggga aaagaaataa
     2701 tgaaaacaga aaagaaacca accaaaaatg ttgttatatc ggagcgtgat t
//
LOCUS       DACEDD010000145         2713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEDD010000145 DACEDD010000000
VERSION     DACEDD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.151"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWO45_003071"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWO45_003071"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0334484.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1062 a          419 c          556 g          676 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ata
//
LOCUS       DACEDD010000146         2711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEDD010000146 DACEDD010000000
VERSION     DACEDD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.152"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IWO45_003072"
     CDS             287..694
                     /locus_tag="IWO45_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334485.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IWO45_003073"
     CDS             708..1109
                     /locus_tag="IWO45_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334486.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKIK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IWO45_003074"
     CDS             1111..1482
                     /locus_tag="IWO45_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334487.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IWO45_003075"
     CDS             complement(1518..1820)
                     /locus_tag="IWO45_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334488.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1914..2708)
                     /locus_tag="IWO45_003076"
     CDS             complement(1914..2708)
                     /locus_tag="IWO45_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAQ0334489.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKGPSEKRKLNISDYTTQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKREILLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISAINSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT          955 a          374 c          412 g          970 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaattaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaaggat caaatttaaa ccataattgt
     1801 gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaaca aatgtttgta
     1861 ttgtaaatag aaaaagatga gctaaattaa atgctcatct tttttgatag atattataaa
     1921 tctaataatt cttgtttctt tttattaaat tcttcttctg taatgattcc gtcatccaat
     1981 agttctttat attttcttat ttctgaagct gaggaaattg ttgatgtatt ggaatttata
     2041 gcagaaatat ttcttctttt taaattataa ataatttcgt ttaaagaatc acttatccta
     2101 tcaacagtat ctttatcaac tgcaacattc aatactttac caagagtatc aatagttaat
     2161 gtacccaaca gtatttctct ttttttacta atattattta aattgtctaa tataatactt
     2221 tgtacattat tgcctatcat tttttgttga gctacaatta aacgtttatt agttaatgcg
     2281 tatgcataat tattgtcatg cttagttgac gagatatagt tatgcaatcc tacaaagcag
     2341 aagattacgt attcgtcact gttaagttga tctgatatta agttaaaatg atgagttgtc
     2401 cattttttag tcattccttt accatagccg tatttaagac aaaattctac catctcttca
     2461 gtagttctca ttctatcatt acctatatca tgattgtttt gtgtagtata atcagatatg
     2521 tttaattttc ttttttctga tggaccctta tctaaatctt ctaaattcaa agatcttatt
     2581 attttataag gagtagtcat accgtaacca gcttttttca tacaattgcc gcaaacaatt
     2641 tgtttatctt tgattttata tgaagttaag ccaattttac ctccgcactt tgcacattgt
     2701 ttactcatat t
//
LOCUS       DACEDD010000147         2707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEDD010000147 DACEDD010000000
VERSION     DACEDD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.153"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            713..2629
                     /gene="ltrA"
                     /locus_tag="IWO45_003077"
     CDS             713..2629
                     /gene="ltrA"
                     /locus_tag="IWO45_003077"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0334490.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
BASE COUNT         1036 a          438 c          566 g          667 t
ORIGIN      
        1 gtgacctgtt tagcctaagg ataaaagagg ctagttccaa cgaacattgg aataacttta
       61 ctatcgagaa atcaaatgac ggggtaacgc cctgaagggt ttccactgat actgcgacat
      121 gcgttagtag gatgtgcatc caaacgcgta tgaagctcgc tgaagtcggc agaagggtac
      181 cgtaaggaaa gacacgatac gtatctacca cgcaagtgct ctagaggtag ggtatgtacc
      241 tgatatgtcg ggtgtcctaa aaagttcaat aaggtgagaa tgatttgatt atctgacaaa
      301 cttgcgaatg gaatgtctga aacgaataac tcatagaaat gtgaggggga agggggatac
      361 gtcctctttc cattgactcg aggttgagta aaaatgtgtt tctatgaaaa accttgcatg
      421 gttaaaggct atggagtagt caacaggcaa aagcaagcac ctaagttgaa atctatggat
      481 agatagtaag gaacagggga agcatctagc acggagggag cataccgaag atgtgtttct
      541 aaggaaaaac cgaaatcggt tataacttag gtgaatagat gtgaaagtag agccacgacc
      601 agtgaaaatt tcgtaatgaa attggaggaa caggctcaag tcgttattta taatataaga
      661 aacctattta aaatcatgga ttctcgtaag actaaaaagg tcacgaacca gaatggagcg
      721 tgataaaacc atgactaaca caaaaggaat tagcttaaag aacaaacaac tcagacacgc
      781 cgaatactac gacatgactg agactttcga tgaattgcac gcaatgagtg ttgcaaaatc
      841 aaattttaaa aatctcatga aattcattat tgatgacaat aatatctttc tagcgtatag
      901 aaatatcaaa aagaataaag gaagtgcaac ggccggatac gatggtgtca ctataaaaga
      961 cattgaaaaa ttggcatgtg gtgacttttt aactattatt aaaaggcgat ttaaggatta
     1021 cagaccaaga aaagttaaaa gagtagagat cccgaaacca aacggaaaac ttcgaccttt
     1081 aggaattcct tcaatatggg acaggatagt ccagcaatgt attctacaaa ttttagaacc
     1141 aatatgcgaa gcaaaattta ataagcatag ctatggattc agaccaaatt gttctacaga
     1201 aaacgccata gcagattgtc ttgctagaat caatcaaagt aagatgaatt atgttgtaga
     1261 tattgatata aaaggattct ttgacgaagt aaaccatacc aaacttatgc gtcaaatatg
     1321 gacaatgggg attcgagata aacaactcct tgtaattatt cgtaagatgt taaaggctcc
     1381 gattgttatg cgaaatggca atgttgtttt tccaaccaag ggtactccac aaggagggat
     1441 tttaagtcca ctgcttgcga atatatgcct gaatgaattt gattggtgga tagctaatca
     1501 atgggaggaa cgaactgtca aagagctgaa acaccactat cgaaaaaacg gagcatggga
     1561 taaaggaaat atctattcga aattaagaag ccacacaagt ttaaaagaga tgtatctggt
     1621 acgctatgca gatgatttta aaattttctg ccgaacagga gaagaagccc aaagggcttt
     1681 ttatgccaca caaaaatggc tcagagaaag attaaagctc ccaatttcaa tggagaaatc
     1741 taaagttacg aacctaaata aagctgaaag tgaattcttg ggtttttcaa taagactcca
     1801 caaaaaggga aaaaagaaag tggctttcac gtacgtttgt cgcaaagcag aagaaaacat
     1861 taaaaataaa ctaaaacaac agattaaaaa aattcaatac gctggagcaa aaaaaaatag
     1921 gcaccgagca ataggtgact acaacatgat ggtcatcggt atccacaatt actatcggat
     1981 agcttcacat tgtagtgtca atttcactga gatttataga agtgttagga cagtaataga
     2041 agcaagactg aaagctagag gaatgacgaa aaatggaatc tatggacaag atcatgaagg
     2101 ataccgtccg tatctaaagt ctaagcaaat gagatatatt gaaggttttc cgatacttcc
     2161 aattggctat attaaaaaaa gaactccact caacaagcgg agaaatctaa ataaatatac
     2221 caaggaaggg agaaagctca ttcatcaaga gcaacaagct atagaaagct ggaaaatcaa
     2281 gtatcttgta aaaggtttta gtaacaataa gaaatacacg gtggaatttt ctgataacaa
     2341 aatatctaag tttatttccc aaaaagggaa atgcgctatc acaggaaacg aacttttgat
     2401 ggaagaaatt gaatgccatc acattaaccc atatcatttg tccaaagacg atagttacaa
     2461 gaaccttgtc attatctcta gagaaatcca tagattaatc catgcaacta gtgaagaaac
     2521 tattctgaaa ctgcttcggt tactgaaact aaatcactat caaattagaa aactaaacac
     2581 gtatcgaaaa aaagcagcta attttccaat atcaatcaaa atgaattaga taggaaacaa
     2641 attatattgt aattaacgat ggaacgccgt gtgcggtgaa agtcgcacgc acggtgtgga
     2701 ccggggg
//
LOCUS       DACEDD010000148         2634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEDD010000148 DACEDD010000000
VERSION     DACEDD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.155"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWO45_003078"
     CDS             5..1150
                     /locus_tag="IWO45_003078"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0334491.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWO45_003079"
     CDS             1321..1779
                     /locus_tag="IWO45_003079"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0334492.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IWO45_003080"
     CDS             1938..2408
                     /locus_tag="IWO45_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0334493.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IWO45_003081"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IWO45_003081"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          862 a          505 c          496 g          771 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc accccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DACEDD010000149         2508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEDD010000149 DACEDD010000000
VERSION     DACEDD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.157"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..230)
                     /locus_tag="IWO45_003082"
     CDS             complement(<1..230)
                     /locus_tag="IWO45_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0334494.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTS"
     gene            complement(342..1685)
                     /locus_tag="IWO45_003083"
     CDS             complement(342..1685)
                     /locus_tag="IWO45_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0334495.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(1819..>2508)
                     /locus_tag="IWO45_003084"
     CDS             complement(1819..>2508)
                     /locus_tag="IWO45_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0334496.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT          742 a          447 c          324 g          995 t
ORIGIN      
        1 gaagttgttg aagactcagc atttgaagtc gtactacttt cacttgataa agttgtcaaa
       61 agttcactac tctctgctgt tacatcggaa ttactcattt cagtcgtctc ggttctttct
      121 tttgcaattt tgtatgttga attttcgctt gccaacacat ggaaaggtgt tgctataaag
      181 agttgggaca ttaatataac tgctgatgca agttttaaag attttttcat ttgatttacc
      241 tctctttttt ctctttaata gtgttttcta ttaaaaatta tgataaatat tttcaatgaa
      301 ttgagtaata cagtatattt cttgatttca tttagtaacg attatgtttc aatgtttaat
      361 cctgttactg cctttaaaat aaaattatag ggttggctaa aactaggatg aaataaataa
      421 tctaacattt tcaatttgtc taatgtaatt tgttcttcta tagcaagaga aaacattgat
      481 aatagtccag ataaatcttc ttgactacaa agttgtacac ctactattct ctttgtattt
      541 ttttcaaagc taattcttaa ttttacacga ctattttttc tcaaaaaaac aggtctaatg
      601 aaatcttcta tttctatata atctatagac tcacttaata atcgtgctgt ttttaaattc
      661 aaacctgctg cgtaaaaatt gtaattaaat atagaaaagc catttgtaaa ttgggaacca
      721 gaaggtgtaa aatcttgatt gtcaatatgt tgagttgcaa tataagctgt tcttagagca
      781 ttagaaatag aaaaatcaac actacttcta ttaatggacg taaaaaaagt tgtagcacaa
      841 tccccaatag catatatatc ttttatattt gtttgctggt attcattagt tagatatgct
      901 ccactaacat ctctcaagag ttcctttttg ccaattggac tattagatag aaatcctata
      961 gcttgtacga ccatatccat agagtattgg gtaccacttg tagtgactat tttttcaatc
     1021 ttttttcttc ctaaatattt tttaggactt tctgatagac aaagatgtat tccatttttt
     1081 gatagtattt cttcaacttt atctgaaaaa ctacggtcgt aataagtcga caacactcta
     1141 tctgcaacat cgaaaagata tacttctttc cctctttttc ttatggcttc agcaatttca
     1201 actccaatat atcctgctcc aataattcct acttttttaa tagtattatc atctaaagct
     1261 ttaaaaatct ctttagcatt tggaaaattc tttatcgaaa aaatattagc taaattattc
     1321 cccggtacat ctaacataat ttgctttgaa cctgtagcta gtataagttt gtcatattct
     1381 tgggaaaatc ttgtgccatc tttttttaca caaataatct ttttattttg aatatcaatt
     1441 ttgtcaactg ctgtctccaa aaacaacttt tttatcttag aatttagttc tcttctattt
     1501 tgataaaaaa gatcttctaa attgctaact tctcttttta tgaacaacga tgtcccacat
     1561 tctaagtaac taatttctga ttttttatct atcagaataa cttcataatt tgaatcacat
     1621 aagtttagaa atcgagctgc agctagcccc gaatggtttg atccaataat tactattttt
     1681 ttcacttaat cacattcttt tcccttctat gaaaagtatc ttcattttta actaacaacc
     1741 tatctcccaa aataaaaaac aaaaagagga agaaaaagat tcttcctctt ttcagagaag
     1801 atttaagaaa ttgattttct attcattctc tttttttcga tttttatacc ctaataaccc
     1861 taaagttcct aacaggatta aaccaccact aatagtaaag atattactat caatctctcc
     1921 cgtcttagga taagcttttg aggactgctt ttgaggactg cttttgcctc tatttttgtt
     1981 ttgcttgcta ccttgattgt cagtcttatt atctttacta acggcttttt cactgtcttt
     2041 ttcgtttgaa gaaacaacta cattagtagg ttcactagta ttaccagatt catcagttgc
     2101 agttactatc acttttgttc ctgcctctaa tttaggaact ttaactgtaa aattaccatt
     2161 tttatcagct gtcccatggt attgactccc gtttggtaat gtaactgtca cctctgcatt
     2221 tggttcagtt ttaccagtaa caatttggtc accctctgta ataggatcaa cttgaggttt
     2281 attcggtgct tttttatctt ctacagaaat agtaacctct acttcttctt tagatccatc
     2341 tggatatgtt actactacaa tccctttttt gtttccagct gtactaacat ctactttctc
     2401 tttccaagta taagtagttc cttttggtag atcttctttg ttcttaattc cctcagatgc
     2461 gtctggctct ttatttaagt ctgtagtcac tttttgtcct tcaggatt
//
LOCUS       DACEDD010000150         2497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEDD010000150 DACEDD010000000
VERSION     DACEDD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.158"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IWO45_003085"
     CDS             complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IWO45_003085"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0334497.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1894..2286)
                     /locus_tag="IWO45_003086"
     CDS             complement(1894..2286)
                     /locus_tag="IWO45_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0334498.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          720 a          550 c          418 g          809 t
ORIGIN      
        1 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
       61 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      121 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      181 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      241 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      301 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      361 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
      421 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
      481 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
      541 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
      601 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
      661 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
      721 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
      781 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
      841 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
      901 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
      961 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
     1021 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
     1081 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
     1141 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
     1201 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
     1261 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
     1321 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
     1381 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
     1441 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
     1501 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
     1561 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
     1621 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
     1681 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
     1741 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
     1801 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
     1861 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
     1921 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
     1981 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
     2041 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
     2101 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
     2161 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
     2221 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
     2281 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
     2341 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
     2401 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
     2461 ttattatact gtactatcct ttattagtgt ttttact
//
LOCUS       DACEDD010000151         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEDD010000151 DACEDD010000000
VERSION     DACEDD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.159"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1014..1304)
                     /locus_tag="IWO45_003087"
     CDS             complement(1014..1304)
                     /locus_tag="IWO45_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334499.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IWO45_003088"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IWO45_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACEDD010000152         2406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEDD010000152 DACEDD010000000
VERSION     DACEDD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.160"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..103)
                     /locus_tag="IWO45_003089"
     CDS             complement(<1..103)
                     /locus_tag="IWO45_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0334500.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(177..458)
                     /locus_tag="IWO45_003090"
                     /pseudo
     CDS             complement(177..458)
                     /locus_tag="IWO45_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            920..2302
                     /locus_tag="IWO45_003091"
                     /pseudo
     CDS             920..2302
                     /locus_tag="IWO45_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          719 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg
     2341 taaaaaacta ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag
     2401 caagaa
//
LOCUS       DACEDD010000153         2397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEDD010000153 DACEDD010000000
VERSION     DACEDD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.161"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            206..2068
                     /locus_tag="IWO45_003092"
     CDS             206..2068
                     /locus_tag="IWO45_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0334501.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IWO45_003093"
     CDS             2068..>2397
                     /locus_tag="IWO45_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0334502.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACEDD010000154         2395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEDD010000154 DACEDD010000000
VERSION     DACEDD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.162"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IWO45_003094"
     CDS             316..570
                     /locus_tag="IWO45_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0334503.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IWO45_003095"
     CDS             571..888
                     /locus_tag="IWO45_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0334504.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="IWO45_003096"
     CDS             1447..2382
                     /locus_tag="IWO45_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334505.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DACEDD010000155         2350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEDD010000155 DACEDD010000000
VERSION     DACEDD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.165"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..543
                     /locus_tag="IWO45_003097"
     CDS             <1..543
                     /locus_tag="IWO45_003097"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0334506.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1016..1588)
                     /locus_tag="IWO45_003098"
     CDS             complement(1016..1588)
                     /locus_tag="IWO45_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0334507.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1604..2209)
                     /locus_tag="IWO45_003099"
     CDS             complement(1604..2209)
                     /locus_tag="IWO45_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0334508.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          467 c          359 g          783 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
      601 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
      661 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
      721 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
      781 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
      841 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
      901 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
      961 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1021 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1081 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1141 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1201 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1261 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     1321 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     1381 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     1441 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     1501 tctctacgca gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     1561 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     1621 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     1681 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     1741 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     1801 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     1861 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     1921 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     1981 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2041 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2101 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2161 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2221 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     2281 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
     2341 cacagtctag
//
LOCUS       DACEDD010000156         2337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEDD010000156 DACEDD010000000
VERSION     DACEDD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.167"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..343
                     /locus_tag="IWO45_003100"
     CDS             131..343
                     /locus_tag="IWO45_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334509.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            505..1023
                     /locus_tag="IWO45_003101"
     CDS             505..1023
                     /locus_tag="IWO45_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAQ0334510.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1030..1542
                     /locus_tag="IWO45_003102"
     CDS             1030..1542
                     /locus_tag="IWO45_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334511.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1813..>2337
                     /locus_tag="IWO45_003103"
     CDS             1813..>2337
                     /locus_tag="IWO45_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0334512.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          890 a          298 c          384 g          765 t
ORIGIN      
        1 aaacactata tattccgaaa agtgagtatc tccattcata aaataatcaa catatttaaa
       61 aaatctaatt agctttttta gtcaatctaa agttctttat tctatgatat aatgttactg
      121 tagaaagttt gtggcggttt ggcaaatcca attgaaggag cgtggtgcct atgggcttat
      181 tagcttatcg gacgtactct gtagaaagga aatgcctgat ttgttctcat tggatcaaat
      241 cattgctatt atcggagcag cagcagtagt tattactgcc gtagcgaaag ctagcgattc
      301 gattttagct tggttaaagt tcaaaaaaga acaaaaaaaa tagagccgtc tcaacttttg
      361 gcgaggtaga cgagctctaa agctttattt acatgccatc cgctttgaac ggactacact
      421 gggcatgtga cagcatgccc tttcatttat attatatctc attcttttat ataatgaaag
      481 taaataaatc atggaggata aaatatggat aaatttaata gattaacatt aataaatcag
      541 tttgaaattt taaaagcttt aaatcctaac gaagaaaaat actatgctga aaaaatagaa
      601 atactaacag aaggttacga atatcactat agtgaaatat ttgaaaatat ttctgatcca
      661 ttgccagaag aagattcgcg gtttgttcta gacatattaa atatgtatag agatattact
      721 tttagtaaaa ataaattaaa agatataaat tcaattgata attattatat acaatttaaa
      781 ggttttgact ttaatagctc taccgaattt aagttagcac tttatgcaca attttttatt
      841 gaaaaattaa ataggtttca agaaatagtt gaagatagtg attttaacac ttttaatagc
      901 cataaaccga tgagaggtac ttatataaaa tttctcgaaa attataatga tttaaaatct
      961 accaataatt atcaatttgg aaacttgtct tatgagatga taagtaaagt tctaagtttg
     1021 tgatgataaa tgaaattaaa tagtataaat gatttatttg ctattatagg tgttattgct
     1081 aacatacttt tttcgtgttt gaattttaga tttactcaaa agaacaaaaa gaacaaactc
     1141 agtatagaac aaatgaaaaa agacattgaa actgatttaa aaaatgacca taaagaaaga
     1201 agaaaaattg aagaatatac ttctttaatg gaaaagtgta aagcaactga tgcaattttt
     1261 agctttttaa gaaaaagtga tgagataatc acacgtgaaa atatagattt agaaaatgag
     1321 attagtaaac ttgaatctat cttttccgat ataaattctc ttaagtattt aatcgtcgaa
     1381 gaaaattcgg atagcttttt agaatttgaa aaatatctaa cagatcttat agataataga
     1441 aataatgaat taatgagaca aaacattgca agattcctta ctaaattagt ttctgaatgg
     1501 aaaagtatta tttctcaaaa tatggataaa ttaagatatt aaggatgtta gttttaaaat
     1561 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
     1621 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
     1681 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
     1741 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
     1801 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
     1861 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
     1921 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
     1981 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
     2041 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
     2101 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
     2161 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
     2221 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
     2281 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgtt
//
LOCUS       DACEDD010000157         2310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEDD010000157 DACEDD010000000
VERSION     DACEDD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.168"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..702
                     /locus_tag="IWO45_003104"
     CDS             25..702
                     /locus_tag="IWO45_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0334513.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(839..1255)
                     /locus_tag="IWO45_003105"
     CDS             complement(839..1255)
                     /locus_tag="IWO45_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334514.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1258..1542)
                     /locus_tag="IWO45_003106"
     CDS             complement(1258..1542)
                     /locus_tag="IWO45_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334515.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(2242..>2310)
                     /locus_tag="IWO45_003107"
     CDS             complement(2242..>2310)
                     /locus_tag="IWO45_003107"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0334516.1"
                     /translation="CSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          747 a          355 c          416 g          792 t
ORIGIN      
        1 ttaacagtca aatatcaaaa tgaattgaat ttagtaccgc tgaaaaattt caatgctaag
       61 cagatggatt tattttttgc attatgcgct cgtatgaaag atcgtggttt gaaaaaagtt
      121 agattttctt tcgaggaatt aaaagagtta agtgattata agatgactgc tacaaaagcc
      181 tttgtcggtg atttagaaaa gctctataaa gatatgctta atcttactta ccgtactgaa
      241 gatgaggatt ctattcgcta ctttgttttg tttactggtt ttgaaatcaa taaaacaaaa
      301 aagtttgttg aagtcagtgt taatccggac ctagaatata ttattaatgg tttaacagct
      361 gaatttagta gatttgaatt atctgctttc acgtcgttgc gttctacgta tgcaaaaact
      421 ctattccgac tgcttatgca gtaccgttct actggttatt atgtagttcg tattgaagag
      481 tttaggagct taatggatat cccagaatac tatcaaatgg gtaacattga tcaaaaagta
      541 ttaaatcctg cattacgaga actaaaaaaa tacttcaatg atcttaaagt cacaaaaata
      601 aaagcaaaaa agggcaataa aatcgccaaa ttagaattta ctttttcaag cctaacgtca
      661 gatttgccaa agatttctct gcgtaactgg ttagaagaat aaataaaaaa gaactcgccc
      721 cccttacatt aagcaagtag acaagtccct tacaaccgac taagattgct agtacattct
      781 agcatattgt atggggcttc taaataaaaa acagatagga ataactccta tctgtttttt
      841 atcgttttag aaaatttgtt gggtcgaaat ttctagaagt gactgtttga ctggtttctt
      901 tttgatgatt ctttggtcga tttcgatttg atggatttag tttagcttcg attttttctt
      961 tcacttcttg atatacttta gaatttgtgc caataagaac gcctgtatct tttagatata
     1021 aatcagcaaa ttctgcgatg atttcagtca tgatggaaat ttcttttccc atagcattta
     1081 gttttgcctg attgctggta gtggtcgtgt tggtatcggg ttcataactc atacataaaa
     1141 agcgaatggc ttccgagtag ttattgatat ctggattttt ttcgatgatg gaatctaaaa
     1201 cgtcaacatg actagggtac aaagtaacat gtttttggac agaagttttt ctcatggtta
     1261 tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat actcgcttaa
     1321 tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat cgaattggtc
     1381 ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt ttagatattt
     1441 atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt tgtctattgt
     1501 tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc tccttttgca
     1561 attatgtata atttatgtat accatacaaa tactatattt gtatagctgt tttttgccaa
     1621 aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa
     1681 acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat
     1741 gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat ttaacagacg
     1801 aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg gaaaaattaa
     1861 tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca tggagcgaat
     1921 ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct aagagttttt
     1981 tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc gaaaaatacg
     2041 agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac ggattttaga
     2101 cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat tgcaaataag
     2161 tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt tttttgtggg
     2221 ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt aactgaacaa
     2281 agcgcttaaa agcccaacaa gaagggagca
//
LOCUS       DACEDD010000158         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEDD010000158 DACEDD010000000
VERSION     DACEDD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.173"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWO45_003108"
     CDS             <1..378
                     /locus_tag="IWO45_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0334517.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWO45_003109"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWO45_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0334518.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IWO45_003110"
     CDS             1859..>2003
                     /locus_tag="IWO45_003110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0334519.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACEDD010000159         1993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEDD010000159 DACEDD010000000
VERSION     DACEDD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.174"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            74..403
                     /locus_tag="IWO45_003111"
     CDS             74..403
                     /locus_tag="IWO45_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0334520.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..680
                     /locus_tag="IWO45_003112"
     CDS             393..680
                     /locus_tag="IWO45_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0334521.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            964..1821
                     /locus_tag="IWO45_003113"
     CDS             964..1821
                     /locus_tag="IWO45_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0334522.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          741 a          266 c          393 g          593 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtggaac gcatgaaaac ttttatgaac
      661 agttgaaaaa attaatctag tgatttgttg tcgttcttaa gaaggacggc ttctttttat
      721 cgaaaatctg attcgtctac atgactaaag tcgcgagtat tctctgcttc tcgataaatt
      781 aaataacgtc attactttga ggaaagctat aaatcaatat atttcagctt ttccttattt
      841 atgtactacc ttttacttat taattataag gtagtacata tatatctttt tttggtagta
      901 actaaaaata aatatgttat aatagtatca aatgaatatt gctaaaagaa aagaggtagg
      961 aacgtggtag cgaagaaata tgtaatcggt aattttaaag gtggcgtagg taaaagtacg
     1021 tgcgcgcaaa tgttagggtt tgaatcagca gttaatgaag gaaaaagaac cttgataatt
     1081 gacttggata tgcaaggaaa tacttctgat gtgatgaact taacgcatat gaacttcact
     1141 aacgaagaag gaggcggaga aggtgaaccg attgtttacg aaaatactgt gactgacgtg
     1201 ttgataaaca atcttccacc taaagaggct gtctataaag taatcaataa cctctatata
     1261 cttccagcag acatgtcctt tgaaatgtat gatgattgga tcaaagacca atataatgat
     1321 tcattatccc aattcaaata tatgaaagaa aagctagaac cattatttga tgagtttgac
     1381 gtcatctatt tagatgtccc accttctatt tctgtatata gtaaatccgc gatgtacatt
     1441 gcggattggg caatcgtcgt cttacagacc caagtaaaat cgatgagaaa tgcgatgcaa
     1501 tatcttgagt atatggactt ttttgtgaaa gaatttgata caaatttata tgttgctggt
     1561 gttattcctt tcatgttgga aagcggtgac gcagtagatc aggaaatgta taaacaagcc
     1621 caagagattt atggggaaca tttgattaaa aatgttgtac tcaaaaatgc gcgcttaaaa
     1681 cgatacgacg gatcaggtat tacgatggag aagacaaaga aaggtctctt aaaacaatgg
     1741 gatcgcaaaa gccacgaatt atttatcagt attttgaaag aattggaaga acacgagaat
     1801 tggtatgcgg aggtggagta attggctgac atcaaaggac ttgatatttt tgggaacaac
     1861 aaaggactcg ccaaaataaa aaaagaagca gaagcaaaga aaaagcagca agcaattaca
     1921 ccatctgaaa ataagcaatt agaagtgcac caagttcaag aaataaaaac ttcctccaat
     1981 gttgaaagac aga
//
LOCUS       DACEDD010000160         1935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEDD010000160 DACEDD010000000
VERSION     DACEDD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.175"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..244)
                     /locus_tag="IWO45_003114"
     CDS             complement(<1..244)
                     /locus_tag="IWO45_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0334523.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            482..1651
                     /locus_tag="IWO45_003115"
     CDS             482..1651
                     /locus_tag="IWO45_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0334524.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          689 a          323 c          300 g          623 t
ORIGIN      
        1 cttgccatac ctcctgaatt tgaccatcag gagtcgtaat gaaaatagta tcattttcat
       61 ctactaattc tcttaagttt aaatattgtt tactagatgt aaaattcgaa gctgctaatg
      121 acgagtcttc gcttttacta taaaatcctg ttacttgtat tttatgttcc tggaaatact
      181 tcccgaatga acaacctacc ttgccggctc caataaaacc aacgttcata attatcacct
      241 ccataaagaa gtgagactac aagtcccatt cttacgatat gagcttaaaa ataaaaatta
      301 aatcaaatat acagtatgtt tttttcaaat atcatccata gttaatttat cataatatcc
      361 catattttaa aataaaagat agcgtaaaat attttgtgta aatagaaatt tagggtagaa
      421 aaaaaagtcc attctgtaaa attaaagtta ccacacagaa attttatagg aggactttct
      481 catgactaaa tttactatag aaattatgca aacactactc aataaagggg atttagatga
      541 attatttcag gatcatttag agcgagcgat taattcactt ctacaagcag agctaactgc
      601 ttttttagat tacgaaaaat atgatcgaaa tggattcaat tcaggaaatt cccgtaacgg
      661 gaattattca cgtacattta aaacagaata tggcgagttg aatttaacta ttcctagaga
      721 tcggaatggc gaattttctc aacaaacact accagcatat aagcgaacca atgattcatt
      781 agaaacgacc attatccaat tatttcaaaa aggcattact atggctgaaa tctccaaatt
      841 aattgaaaaa atgtatggtc accactatac gccacaaaca atctccaaca tgagtaaatt
      901 ggtggctgaa gatgttttag cttttaaaga aagaacgtta gaagccaatt attccgttat
      961 atttatggat gcgacgcata tccctgtgaa gcgacagact gtttcgaagg aagcagtgta
     1021 tattacgatt ggtattcgtt tggatggaac caaagaagtt cttgggttta ctattgcccc
     1081 aactgagtct gcttatattt ggaaagaagt tcttcaagat cttagaaaac gtgggttaga
     1141 agaagtttta ttagtagtga cagacggatt aagctgtatt gaagaaagta tccatagtgt
     1201 gtatccgaat gcccaatttc aacaatgttg tgtgcatgta tctagaaata tcgctcataa
     1261 agttcgtgtt cgaggtcgaa aagaaatttg tgaggatttc aaattggttt accaagcgaa
     1321 ttcaaaagaa gaggcattgg atcacatcga ctttatgact aggaaatgga aaaagcagta
     1381 tccaagagtc gtcaatttac tcttgaatcc tgccctatta accttttata atttccctca
     1441 cgccatcaga cgaacaattt attcgacgaa cctgattgaa ggctttaata agcagctaaa
     1501 acgatatact cgaagaaaag aacaattccc taatgaagaa tctctagaga gattcttcat
     1561 ttctcaattt aatcaatata accaaaaatt tttaggtaga attcacaagg gatttaaaga
     1621 aattcaggat acattagagt cgatgattta actgtaatta acagaatgga ttttccattt
     1681 acatataatt cttgacgcta ccataatcta aataaccatc atattcatct actacttata
     1741 taattgagtt ttattaaact ttttaagttt ctattctatc aaatatttaa acaatctctg
     1801 catccgaaaa gactacctcg tatagtttta ctaacaatat ttttattagc gtgttcaaat
     1861 cctgatttct tgtatcaatt attttctgtt cactttcaat aagaaaaaag tctttacttc
     1921 catggataat aataa
//
LOCUS       DACEDD010000161         1930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEDD010000161 DACEDD010000000
VERSION     DACEDD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.176"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..596
                     /locus_tag="IWO45_003116"
     CDS             <1..596
                     /locus_tag="IWO45_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0334525.1"
                     /translation="KQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPVSFKPI
                     YGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGKATD
                     WHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEE
                     SQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
     gene            796..999
                     /locus_tag="IWO45_003117"
     CDS             796..999
                     /locus_tag="IWO45_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334526.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1559..>1930
                     /locus_tag="IWO45_003118"
     CDS             1559..>1930
                     /locus_tag="IWO45_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0334527.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSM"
BASE COUNT          625 a          293 c          455 g          557 t
ORIGIN      
        1 tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca gatattgctt
       61 gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt gaccctgtgt
      121 cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg ggtagtcgtg
      181 ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacga aaaaaacaga
      241 ttgtaccaaa agaaatcgaa acatggtggc gtatagaaat gcagctaaga cgtggcaaag
      301 caacagattg gcatgcgatg gttcgggaaa gtttagatag ctttgcaagt cctcattact
      361 tgcctggaga tgtaaaacct gttgatagaa ttatgataaa agggctgaat caagatcatt
      421 cagagtgggc gtatattagc agaaatttga aatatagatt gcgcaaattg ttgaaagaag
      481 aaagccaaaa cgatgagttg acgaatcatt tacgagagac gtttaaggaa tctgcaaacg
      541 acttgaaaat agaattagac acgtggcttt tgggtttgga cgtgacagag aagtgatacc
      601 gaataagaaa agtcagatga gtgagagtaa atttacgtca atgtttaatg acgtgagttt
      661 acgataagcg attattttgc gtgaagcaaa ataaagcgta aaaggagttg agagcgtgac
      721 acgctttcgg ctcctttttt cgttgtcact tgtgacagcg aataccctcg gagagcattt
      781 ggaattgtga cagccatgtc acaattagga aaatgttact ctttgtcaaa gagtaacaga
      841 aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggttcacctt agggtttagt
      901 atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg tgagttaact
      961 gatagccaaa aaatcaatgg tttgtctggt agaaaataat tagcagattt acaagtggga
     1021 aataaaaagc tgaaagaaga aaattgcatt tcaaactaca ttttctacaa tagctctaaa
     1081 atggcatatt ttgcgttctg agcggtttta gcgtttattt cgtttagtta tcggcacaat
     1141 cggtgaaaca cgttagagga gcgaaaatgg agcctgtaag gcgactggta gcggaacggt
     1201 cgtgttagat tgtgtagccg atagctgaac gaaataaatg ctggcagcgc cattctattt
     1261 cggcaggagg aggctcaagg ggttcgggga atgaaattcc ctgaggtctt tttatttttt
     1321 ctagaggagt gctataatca aagaaaatga gaaaaaagag taaaaaaata gtcgagcagt
     1381 gttgacgcac tctcgacctt tacggaaact acttcaccta gttccgtaac tcaagttcat
     1441 tatacgaata tttttaggtt tcttcaagct agagattttt ggatgtggat ttttagagtt
     1501 acgtgaactc tggaaattct ttccaagaat ctcttttttc gttgaggagg agctattttt
     1561 gagtaaaaaa ggcataatcg taaatgagtg ggaaaaactt aaccgtattt ctaacaggcg
     1621 gttaagtgtt gacgataagg tacaccccct aaaattaagt tggtcaatcg atcgaattac
     1681 aatcgtcgga aaactgaatg ataccatagc ttattggacg caaaatggag ttgtgtttgt
     1741 cgattttgag caattaatgc gtttgaatga aaatcaaggg tatttgaagt cggttggtac
     1801 aaatggttgg caattatttg accaatatgg agaagatatc gcctatatcg aaatgttaaa
     1861 atttcaccct gacaaaggtc gtattgattt taatccaaat aaaattggcg agtttttaaa
     1921 ggggtcgatg
//
LOCUS       DACEDD010000162         1891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEDD010000162 DACEDD010000000
VERSION     DACEDD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.177"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            192..290
                     /locus_tag="IWO45_003119"
                     /pseudo
     CDS             192..290
                     /locus_tag="IWO45_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(513..743)
                     /locus_tag="IWO45_003120"
     CDS             complement(513..743)
                     /locus_tag="IWO45_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0334528.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(973..1791)
                     /locus_tag="IWO45_003121"
     CDS             complement(973..1791)
                     /locus_tag="IWO45_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0334529.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          667 a          308 c          274 g          642 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg
      241 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag
      301 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
      361 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      421 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      481 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      541 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      601 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      661 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      721 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      781 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      841 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      901 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      961 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
     1021 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
     1081 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
     1141 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
     1201 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
     1261 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1321 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1381 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1441 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1501 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1561 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1621 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1681 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1741 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1801 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1861 ccatttttat ctttactttt atgaaagcgg a
//
LOCUS       DACEDD010000163         1883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEDD010000163 DACEDD010000000
VERSION     DACEDD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.178"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWO45_003122"
     CDS             <1..153
                     /locus_tag="IWO45_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0334530.1"
                     /translation="VGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWY
                     LNIPKR"
     gene            268..1476
                     /locus_tag="IWO45_003123"
     CDS             268..1476
                     /locus_tag="IWO45_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334531.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
BASE COUNT          708 a          244 c          316 g          615 t
ORIGIN      
        1 gttggctatc atgtatcttt taacatgcgc aatttcccta ataagaaagt atacgtcatg
       61 atgcgggcaa cgaatgatcc agaaggaaac actaaaggcg gtgcgcaaga tttccatgac
      121 aaacgttggt atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagcagtac
      181 atagaattga aaattaacgt taaatcaaaa aaatatttac aagatgttta tttaagtaga
      241 taataaaaga aaaaagcagg tgaaattgtg acagcagaaa ttggtattat gaataaaagt
      301 ggaatcgtgt tagcttcaga ttcagcatct acgattggag atagcaaagt atataatact
      361 gcaaaaaaat tatttacttt ggattccatg cattctgtag gtatcatgat atatggtaat
      421 gctgaattta atggtattcc ttgggaaata atcattactc aatataagaa aagcattggt
      481 agttctgttt ttaatacttt ggaagaatac gcggataatt ttatagaatt tgtcaaaaca
      541 gcatctttta ttagaagtga acagaccgag caagaacaaa tgattggggt ttttcaaaaa
      601 ataatttcag gtttatttga gagtatagaa gtagatatta actttttaat aagccaaggt
      661 acacaaatag acaaagatgt cctagtgaag ttattacaaa caaaaatgaa taccaatttg
      721 tctcaacagt cccagacatt cattttagat attgaaaagg cgttatttct tagtaactat
      781 ggagaaatat taaaagatat tcttaatagt atttctacaa tggaaggtgt ttcagaagct
      841 atatcagagg aaattcaaag ttacgtttat gaaattataa ttagagacga tgtttattct
      901 tcccctacag gaatagttat agcaggatat ggaagaatgg atattttccc taaactatat
      961 tcttataata tgtttggatt tgttatgaat attctgaaat actcagaata tgaaagtgct
     1021 caaataggaa atgacaatgg ttcattaaga tctactatat taccatttgc tcagtctgat
     1081 gtggtaaata ctgtagttca aggggtagat ccacaaatta ctaattatct gtcgagtcaa
     1141 gtcgatagtt ttgacgataa cggaaaaaat acctatatta atattataaa gaatatttca
     1201 gagtttcaac aaaatcaatt catatttccg ttactaaaca tgattgcttt actcccagtt
     1261 gaagaaacag caattatcgc agagacttta ttaaatttaa ccagttttaa acgaaaatat
     1321 actacttctg tagaaacagt aggtggccca attgatgttt tagctattac acctaatgat
     1381 ggccctattt ggattaaaag aaaacattat tttgatattg ataataatat tggatacaga
     1441 ttgcgaaagg agagagcgaa tgattacaat aattaaacct caggaaaaat cccctgtttt
     1501 gaaatttaat gagagcaatc tagcccataa taaaatgagt ataactgata cagtatttga
     1561 tgtaatggtt gagcaacaaa aaaaaattaa taataacaat agacaaagaa aagaaattaa
     1621 ccaatattaa tgttttttct gttgcgtttt ttgcagtcat aatttaaaac ctcgctcaat
     1681 cttgggcgag gtcttttttt atttttctga aaagttaaag taaataatag cataaaataa
     1741 tatattttac aaagaataag tacaatctag ttttttgcta ttaaatgtgt aataataaat
     1801 atgccatcac aacatgaaga atgaaatcca ttattatcta gtctagtcca ttctttttgt
     1861 ttgcagtatt tgtgatggct ttc
//
LOCUS       DACEDD010000164         1770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEDD010000164 DACEDD010000000
VERSION     DACEDD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.181"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            197..424
                     /locus_tag="IWO45_003124"
     CDS             197..424
                     /locus_tag="IWO45_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0334532.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            547..873
                     /locus_tag="IWO45_003125"
     CDS             547..873
                     /locus_tag="IWO45_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0334533.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            941..1654
                     /locus_tag="IWO45_003126"
     CDS             941..1654
                     /locus_tag="IWO45_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0334534.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          686 a          273 c          373 g          438 t
ORIGIN      
        1 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
       61 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
      121 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
      181 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
      241 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
      301 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
      361 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
      421 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
      481 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
      541 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
      601 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
      661 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
      721 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
      781 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
      841 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
      901 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
      961 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     1021 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     1081 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
     1141 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
     1201 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
     1261 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
     1321 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
     1381 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
     1441 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
     1501 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
     1561 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
     1621 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
     1681 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
     1741 aacgcaaaga ggtgcatctc tgatttggtt
//
LOCUS       DACEDD010000165         1655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEDD010000165 DACEDD010000000
VERSION     DACEDD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.182"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(188..1207)
                     /locus_tag="IWO45_003127"
     CDS             complement(188..1207)
                     /locus_tag="IWO45_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0334535.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1218..1424)
                     /locus_tag="IWO45_003128"
     CDS             complement(1218..1424)
                     /locus_tag="IWO45_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0334536.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1557..>1655)
                     /locus_tag="IWO45_003129"
     CDS             complement(1557..>1655)
                     /locus_tag="IWO45_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0334537.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          469 a          338 c          279 g          569 t
ORIGIN      
        1 ggttgttgcc acataaagcc ataaacaatc aaccagtaga atgtgtggca acaaacctgt
       61 tatcgcatag cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac
      121 tattttatcg aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga
      181 gctattttta tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac
      241 cgcctttagt gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat
      301 tagggaagtt acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag
      361 cttgatttac atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag
      421 tattgtaatc cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta
      481 catgcaaatt tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat
      541 ctgtcagtgt aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag
      601 aagttgttgg attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac
      661 ttacgcccat ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg
      721 ttacccataa atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa
      781 taccatattt cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa
      841 acgttgcttt atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat
      901 acgaaccagc accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt
      961 ctcccacaat ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat
     1021 tgcgtcctgt tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta
     1081 cttgtgagct accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc
     1141 cgttaattgg caataaaaaa agagccgtta acaggctcat cgcagtaata gtaattttct
     1201 ttttcatatc tatcctccta tttatctgat gaaattttct tatacgttcg atttgataca
     1261 cctaaaatac tccctagaaa tgctccaaaa cctgtaataa tcatcaccgt tatatctgtg
     1321 taccgccagt taacaccttt tccgacaaga ccgacaaaag tcgctaaagc ggggataact
     1381 actatagcga tccatttcaa aatatcaaag gttctattgt tcatactatc cacctccctt
     1441 cattggttgg gctactttct ttttcaatcg cttgttttct tctatcaata tttctctttc
     1501 tgaaagcaag gcatcaattt gcccgtccag ttgttcaatt ttatctttct taattgtcaa
     1561 attaagttag acagatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga
     1621 taatccagtg attttctagg aatattattt cgttt
//
LOCUS       DACEDD010000166         1560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEDD010000166 DACEDD010000000
VERSION     DACEDD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.183"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="IWO45_003130"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="IWO45_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAQ0334538.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            153..887
                     /locus_tag="IWO45_003131"
     CDS             153..887
                     /locus_tag="IWO45_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ0334539.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          583 a          246 c          211 g          520 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc
      181 aaaactttat tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata
      241 caaatgataa tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta
      301 aaaggtgtaa ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa
      361 acaaacttat tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt
      421 ttcctaaaaa taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata
      481 taattcgaaa aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg
      541 gatttgctaa aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag
      601 ttgatatatc catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta
      661 atagctcgtt aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac
      721 aatatgaaaa ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa
      781 accaatttta tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg
      841 aacaattctt gtctcttttc aatagctata aattatttaa tagataagtt aaggggtgca
      901 taaactgcat cccttaactt atcttatttt gcttaaacaa tcttggcccg taaactgcat
      961 aactcatatg taaatcgctc ctttttaggt ggcacaaatg tgaggcattt tcactatttc
     1021 cggcaattac ttccatgtaa agtataaccc actatacttt gcaagcaaag tgtgggctcg
     1081 ccgaggggtc ttgcagacgg ctctttcggc tcttttgagc cgagtatcag cgcaccctta
     1141 tggtgcaatt agctgatacc tgtttgacga tttgctgccc taccttttcg gtaggtatgc
     1201 aaacctcaaa ccaaaataaa aacccccttc gacttcgtca gggggttttt atttatataa
     1261 atctccgtaa ataatcgcga tttaataaca tcttcaattt tatttttttg cgtattttag
     1321 aaatgttttc actaaaaaat atattataca aaaaatacag taatcgatta taattctttg
     1381 ccagttaaat atcatatcgt tattaaaata tcggaaaaaa atatcaacaa caataaatgc
     1441 aaaagaagac cataaaaatt gtttattact catacatact ctccatttct atcattttta
     1501 aatttaccag tcatctctca aaaatctcct agctattttt cttttttaac cacataccgt
//
LOCUS       DACEDD010000167         1534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEDD010000167 DACEDD010000000
VERSION     DACEDD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.184"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            96..506
                     /locus_tag="IWO45_003132"
     CDS             96..506
                     /locus_tag="IWO45_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0334540.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            520..876
                     /locus_tag="IWO45_003133"
     CDS             520..876
                     /locus_tag="IWO45_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0334541.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          564 a          227 c          281 g          462 t
ORIGIN      
        1 ttattttgat ttttttttat attttattag ctaataattg gtattttaaa agaaattact
       61 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
      121 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      181 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga
      241 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      301 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      361 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      421 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      481 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      541 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      601 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      661 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      721 attgcgattg acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat
      781 accaacctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      841 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      901 ctgagcattc catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      961 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
     1021 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1081 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
     1141 ttgttgacat gttagaaatg acatgataag ataactgagt cgttaaaaaa ggaccgcttg
     1201 cgaagcttga ttctatcgaa aaaacttcaa aaaagttctt gacaaataac aaacgacaag
     1261 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
     1321 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctgcggca gccaaaaacg
     1381 aatcgggaaa cactcctgat gaagtagacc tttgaaaaat gaacaaagta agacaaacca
     1441 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca tttttaacaa gcaagcaata
     1501 tgctagcaaa caaattgagc ttaacaatcg caag
//
LOCUS       DACEDD010000168         1522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEDD010000168 DACEDD010000000
VERSION     DACEDD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.185"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..271
                     /locus_tag="IWO45_003134"
                     /pseudo
     CDS             161..271
                     /locus_tag="IWO45_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(368..844)
                     /locus_tag="IWO45_003135"
     CDS             complement(368..844)
                     /locus_tag="IWO45_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0334542.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(857..>1522)
                     /locus_tag="IWO45_003136"
     CDS             complement(857..>1522)
                     /locus_tag="IWO45_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0334543.1"
                     /translation="MYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWL
                     YVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWA
                     FIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIES
                     VEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILG
                     KDN"
BASE COUNT          528 a          278 c          196 g          520 t
ORIGIN      
        1 catctttcag cattttacta tatttggtat atagtgctaa ggttcatatt cgattgacaa
       61 gagaatataa attgatctat ttctatttcc taagttgagt aaacataact ttgcccttta
      121 taaatatctt ccctctctct cttctctcta tattttatat ttgaataatc atatcaaggt
      181 attccaacga aatgccaatg gatttcgaag cttttataac tttaaattac ggatcatgat
      241 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
      301 ctgttcacca acttcatttg acaaatatcc tatttagctt attttatagc tttttatata
      361 cactttttta aaaagtgatt tttccacatg aattcccatg agcaattcga ccattatcta
      421 ttattaaatc attgaactga tttttctccg gaaaaacaac tataacagaa gtatctttac
      481 cagattttat tattttgtca aaatccacat caaccaacga ggtttcagga gtaacctcat
      541 cattttcttg aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta tcaattccaa
      601 tatgaatcaa ataatcaatg cctgagttgc tttttatatt aatagcatgt ttagtaggaa
      661 atatatttgt tactcgacca gccaatggag cataaatttt tgaaccatca ggtatgacgg
      721 caaaaccatc acctagtaat ttttgtgaaa agacaggatc atttacttct gtgatggaaa
      781 tgatttttcc ggaacaaggt gcataaaaag tttcttgttt tttctttttg aaaatttcaa
      841 acatattaaa aacctcctaa ttatcttttc ctaatatttg attgatttct tgacttaata
      901 agtcagcttt ccctccaaaa acagcttgga tgccaccctt cacttcgaag acagcagttg
      961 caccagtgtc ttttattgtt tgtttttgaa taatctcccc attcgtaaca gcaacacgca
     1021 aacgagttgc acaagcttca acagactcaa tattttcttt tccaccaaga gcttcaataa
     1081 tgataagaga ctcttcatgt aatgatgtat ttttgtttat tactttttca gaatgaactt
     1141 ctgattcttc ttccattcca ggaatagcta ccttaaattt tttgataaag aatgtaaaaa
     1201 caaagaaata tataaatgcc catgcaatac caaaaggaat taattttaac caattagttt
     1261 tgtcattccc ttgaagtaca ccgaataata aaaagtcgat caaaccacct gaaaaggaat
     1321 tccctacacg aatatttaag atatcagcaa aataaaaaga taatccatct aagaatgcat
     1381 gaatcacata aagccaagga gcaacaaata aaaacatata ttcaactggt tcagtaattc
     1441 cagttaaaaa tgatgtaagg gccccgctaa aataaagccc accattcttt ttacgatttt
     1501 tcttaggaat tgcacgatac at
//
LOCUS       DACEDD010000169         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEDD010000169 DACEDD010000000
VERSION     DACEDD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.187"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..1040
                     /locus_tag="IWO45_003137"
     CDS             108..1040
                     /locus_tag="IWO45_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0334544.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IWO45_003138"
     CDS             1112..>1456
                     /locus_tag="IWO45_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334545.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACEDD010000170         1445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEDD010000170 DACEDD010000000
VERSION     DACEDD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.188"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1445)
                     /locus_tag="IWO45_003139"
     rRNA            complement(<1..>1445)
                     /locus_tag="IWO45_003139"
                     /product="23S ribosomal RNA"
BASE COUNT          307 a          428 c          283 g          427 t
ORIGIN      
        1 taagaaaaga agcatttgac tcctcttcag acttactgct tggacagaca tttccgatcg
       61 tctgcattcc ttagcctcct gcgtccctcc attgctcaaa caaaaacaac gggtacagga
      121 atatcaacct gttatccatc gcctacgcct gtcggcctcg gcttaggtcc cgactaaccc
      181 tgggcggacg agccttcccc aggaaacctt agtcattcgg tggacaggat tctcacctgt
      241 ctttcgctac tcataccggc attctcactt ctaagcgctc cagcagtcct cacgatctgc
      301 cttcaacgcc cttagaacgc tctcctacca atacacctaa aggtgtactc cacagcttcg
      361 gtaatatgtt tagccccggt acattttcgg cgcagggtca ctcgactagt gagctattac
      421 gcactcttta aatggtggct gcttctaagc caacatccta gttgtctgtg caaccccaca
      481 tccttttcca cttaacatat attttgggac cttagctggt ggtctgggct gtttcccttt
      541 cgactatgga tcttatcact cacagtctga ctcccggata tgaatgaatg gcattcggag
      601 tttatctgaa ttcggtaacc cgagatgggc ccctagtcca aacagtgctc tacctccatc
      661 attctcaatt ccgaggctag ccctaaagct atttcggaga gaaccagcta tctccaagtt
      721 cgtttggaat ttctccgcta cccacacctc atccccgcac ttttcaacgt acgtgggttc
      781 ggtcctccag tgcgttttac cgcaccttca acctggacat gggtagatca catggtttcg
      841 ggtctacgac tacatactca aacgccctat tcagactcgc tttcgctgcg gctccgtctc
      901 ttcaacttaa cctcgcatgc aatcgtaact cgccggttca ttctacaaaa ggcacgccat
      961 cacccattaa cgggctttga cttgttgtag gcacacggtt tcaggttcta tttcactccc
     1021 cttccggggt gcttttcacc tttccctcac ggtactggtt cactatcggt cactagggag
     1081 tatttagcct tgggagatgg tcctcccgga ttccgacgga atttctcgtg ttccgccgta
     1141 ctcaggatcc tcctaggtgt cttccacatt tcgtctacgg ggtttttacc ctctttgact
     1201 gacttttcca agtcattcga ctatctgaaa gaactaccat attggagtcc tacaacccca
     1261 acaagcaagc ttgctggttt gggcttttcc cgtttcgctc gccgctactc agggaatcga
     1321 attttctttc tcttcctgca ggtacttaga tgtttcagtt ctctgcgtct acctcgaatg
     1381 tgctatgtat tcacacaatc gtaacatcct attaaagatg ttgggttccc ccattcggaa
     1441 atctc
//
LOCUS       DACEDD010000171         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEDD010000171 DACEDD010000000
VERSION     DACEDD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.189"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..618
                     /locus_tag="IWO45_003140"
     CDS             <1..618
                     /locus_tag="IWO45_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0334546.1"
                     /translation="PFYKEEESLMPYFALAAQRAGAVGIRANSVRDIIEIRKCVSLPM
                     IGIIKRDYPPESPFITATMREVDELVETGVEVIALDCTLRNRHDGLSINSFITKIKEK
                     YPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGIC
                     VIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            632..>1425
                     /locus_tag="IWO45_003141"
     CDS             632..>1425
                     /locus_tag="IWO45_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0334547.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAG"
BASE COUNT          451 a          202 c          300 g          472 t
ORIGIN      
        1 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
       61 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
      121 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
      181 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
      241 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
      301 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
      361 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
      421 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
      481 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
      541 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
      601 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
      661 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
      721 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
      781 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
      841 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
      901 tggtggagta gcttatctag tatatactgc aactattagt gggtttcttg aacttttttc
      961 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     1021 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     1081 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     1141 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     1201 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     1261 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     1321 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     1381 ccacactggt ctgtttactt atggtactag attttttgca ggaag
//
LOCUS       DACEDD010000172         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEDD010000172 DACEDD010000000
VERSION     DACEDD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.190"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWO45_003142"
     CDS             complement(150..1337)
                     /locus_tag="IWO45_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0334548.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEDD010000173         1374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEDD010000173 DACEDD010000000
VERSION     DACEDD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.192"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            39..1340
                     /locus_tag="IWO45_003143"
     CDS             39..1340
                     /locus_tag="IWO45_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0334549.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          275 c          253 g          398 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DACEDD010000174         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEDD010000174 DACEDD010000000
VERSION     DACEDD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.193"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1364
                     /locus_tag="IWO45_003144"
     CDS             82..>1364
                     /locus_tag="IWO45_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0334550.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DACEDD010000175         1340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEDD010000175 DACEDD010000000
VERSION     DACEDD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.195"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(740..1081)
                     /locus_tag="IWO45_003145"
     CDS             complement(740..1081)
                     /locus_tag="IWO45_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0334551.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1081..1311)
                     /locus_tag="IWO45_003146"
     CDS             complement(1081..1311)
                     /locus_tag="IWO45_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0334552.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          411 a          224 c          196 g          509 t
ORIGIN      
        1 tattgatagt gtacctaaaa gctctactga cataagatcg tgtaatcggt aaagtaattt
       61 tattaattat acgggatgta atcaattaga gaataaacta atcataaaag gtacttttac
      121 attcataatt gtcgctttga gattctactt tggttcacta tctgacccaa ttgcacattt
      181 tcgatcatta caagtattgg tgtcgttaga acaactaaat aaaaactagc ggtagatttt
      241 tcagaatcta tgctaatttc tgtatggatg ttccagtgat ggcaaatttc tcttttcatt
      301 gtttttcttg tcgcatgata tgcctttttg tgttattcag ttaaactata gaaattcttt
      361 cctagacaga tcttgatccc aaattttacg tttttgagca tcgatatagt ttaaaatgac
      421 attactcata tctaaagagt ttagattttt taggactaaa actgtcagtt gttctttttc
      481 ttctttctgt taaagtattg tcgcgacttt tcgtatacgt taagatatgt cgtgaaaaaa
      541 caagttttgt gacacaaaaa aacgaatgac ttgaggaaat cacctcaatc attcgtttaa
      601 aatgctatat attctttagt ttaagatacg gaaattaatg ccatcgacca cactagtaga
      661 atgaacgaca tttacttggc ctttttttaa tttcgttatg tttagcatat aaaaatatta
      721 tagagtttac ttacaatgct taaaacatat actgtataat ctgatcaatt ttagccatgt
      781 cttgcaatgg taagttttct atttttttga gttttcgttc cttaaaatca agagatttta
      841 attgtgtaat aattacttgt ccttttatat ctagctcttc aggtaatgag taacgtgtcg
      901 gtaaattctt tatagtggaa gtaattggac atatgaccga aaacattgtt ttacggttaa
      961 attcataacg agagacgact agtcctggtc gtcttttttg aatttctttt cctacggagg
     1021 gatcaaaatc aatccataca atatcacctt ttttaggaat ataaattctt tcttctgaca
     1081 ctataaaatt tctctccctt caggagttaa atcttcccat tcgtctttct cataatactc
     1141 ggcctcttcc ccaccactaa atggatcttc aatttttgga actaacgtaa ttgttccatc
     1201 atcagaatac acaacgatat attcttggtt ttctgaaggt ttctttccgt tatcagaagg
     1261 taaagttacc actacagaac ttccttgtaa tctagatttt gtaacaagca ttatcatcac
     1321 tcccttaata aaagtatact
//
LOCUS       DACEDD010000176         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEDD010000176 DACEDD010000000
VERSION     DACEDD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.196"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWO45_003147"
     CDS             <1..1244
                     /locus_tag="IWO45_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0334553.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACEDD010000177         1287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEDD010000177 DACEDD010000000
VERSION     DACEDD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.197"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(338..411)
                     /locus_tag="IWO45_003148"
     tRNA            complement(338..411)
                     /locus_tag="IWO45_003148"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Ile,seq:cat)
     gene            complement(966..1280)
                     /locus_tag="IWO45_003149"
     CDS             complement(966..1280)
                     /locus_tag="IWO45_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334554.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          476 a          201 c          194 g          416 t
ORIGIN      
        1 tcccattatt ttttacagtt atatggtatg ctttttaatg gcttcaaata taaaagagtt
       61 taaagcgtaa cacacttatg gggaagtggt ttttggggaa cgctttaaac tcttctttat
      121 tattatctca caatataacc caaatgtctt tccatttaaa aatcaaagta aaacttttca
      181 aatatacaga agtataacta tgtgaaacat cctttcatta atccataaaa ggatacataa
      241 aaaagccact catttgagtg gcaatgaaga aaagctttag cttgtataat actcttcaaa
      301 aaattctaac acagaacgat tcaattggct acattaatgt accctgtagg actcgaacct
      361 acgaccggac ggttatgagc cgtctgctct gaccaactga gctaagggta gtggttgttg
      421 ccacataaag ccataaacaa tcaaccagta gaatgcgtgg caacaaacct gttatcgcat
      481 atcttggagt gtgactattt atgagtgata gtgaagatat gcgtctccat atatgtatgg
      541 ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca
      601 aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat
      661 tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa
      721 gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac
      781 aattatggtt taagcatgtt ccttcaaata agttttttaa ttataaactc tagagaaatt
      841 aattcatcct aacataaaat aattgttcac tcaaaagttt ttctagaaca attaaaacag
      901 caggcttcac attcgctata catttgtaag aagtaataat tcctgtacta aaaggcataa
      961 ttgctttaat tattttttga cttctcttca aacacaagaa gtagttgtgt aataattgca
     1021 gaaataagga ttccaataat agatataaaa tttgacatgg aaacgtctat aacattaaaa
     1081 gaataaagtg ttgaagaaag taaactgatt gaaataaccc ctccgtaaaa aatagtctgt
     1141 gaatataccc actttttttc agaagacgta cttcgtttgg ttctaaaacc tatccctgca
     1201 tttctactct tatcaaaaaa ccaaatattt agaagtaata atattgctat tattaagata
     1261 aaattactgt aaaaatccaa aagctat
//
LOCUS       DACEDD010000178         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEDD010000178 DACEDD010000000
VERSION     DACEDD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.198"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWO45_003150"
     CDS             141..398
                     /locus_tag="IWO45_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334555.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWO45_003151"
     CDS             complement(630..1043)
                     /locus_tag="IWO45_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0334556.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          487 a          159 c          159 g          471 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaat
//
LOCUS       DACEDD010000179         1255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEDD010000179 DACEDD010000000
VERSION     DACEDD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.200"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..>1255
                     /locus_tag="IWO45_003152"
     CDS             52..>1255
                     /locus_tag="IWO45_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0334557.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIK"
BASE COUNT          387 a          250 c          246 g          372 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaag
//
LOCUS       DACEDD010000180         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEDD010000180 DACEDD010000000
VERSION     DACEDD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.201"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWO45_003153"
     CDS             200..742
                     /locus_tag="IWO45_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334558.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWO45_003154"
     CDS             832..>1246
                     /locus_tag="IWO45_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0334559.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACEDD010000181         1240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEDD010000181 DACEDD010000000
VERSION     DACEDD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.202"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            69..503
                     /locus_tag="IWO45_003155"
     CDS             69..503
                     /locus_tag="IWO45_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0334560.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(581..1114)
                     /locus_tag="IWO45_003156"
     CDS             complement(581..1114)
                     /locus_tag="IWO45_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0334561.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
BASE COUNT          379 a          246 c          223 g          392 t
ORIGIN      
        1 gaaaacttgc catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg
       61 gctagaccat gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg
      121 tacggatcag gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag
      181 acaaagatga agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag
      241 gaactgtacg tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc
      301 tttctgaata cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg
      361 ctaagaatca aggctataat acgattttat taggggcaca gtccacagct gaaacttttt
      421 acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc atgcctcata
      481 tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt
      541 tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct
      601 tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga
      661 tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat
      721 tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag
      781 gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa
      841 aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat
      901 tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc
      961 catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt
     1021 ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga
     1081 tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt
     1141 gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga
     1201 gctttgccta gaaaggattc ctattgtttc gatttttctc
//
LOCUS       DACEDD010000182         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEDD010000182 DACEDD010000000
VERSION     DACEDD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.203"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1231
                     /locus_tag="IWO45_003157"
     rRNA            <1..>1231
                     /locus_tag="IWO45_003157"
                     /product="23S ribosomal RNA"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DACEDD010000183         1192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEDD010000183 DACEDD010000000
VERSION     DACEDD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.204"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWO45_003158"
     CDS             <1..1076
                     /locus_tag="IWO45_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0334562.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DACEDD010000184         1185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEDD010000184 DACEDD010000000
VERSION     DACEDD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.205"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            225..1100
                     /locus_tag="IWO45_003159"
                     /note="aph(2'')-Ia"
     CDS             225..1100
                     /locus_tag="IWO45_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0334563.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          516 a           86 c          195 g          388 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
      301 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
      361 aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta
      421 atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt
      481 ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag
      541 aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt
      601 atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat
      661 tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt
      721 ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg
      781 attttagttg taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt
      841 ttggagattc tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg
      901 aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata
      961 ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg
     1021 tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata
     1081 aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa gacattagta
     1141 ttaaatagtt taaaaaaatg aaaaataata aaggaagtga gtcaa
//
LOCUS       DACEDD010000185         1137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEDD010000185 DACEDD010000000
VERSION     DACEDD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.206"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(180..>1137)
                     /locus_tag="IWO45_003160"
     CDS             complement(180..>1137)
                     /locus_tag="IWO45_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0334564.1"
                     /translation="DRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNY
                     HKVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDP
                     MVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNE
                     ETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGR
                     EHEERYMDEKGIVQDDYRIEFIGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFK
                     NRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK"
BASE COUNT          332 a          242 c          174 g          389 t
ORIGIN      
        1 ttttcgaata aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
       61 aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca
      121 caacctcttt tatttcaaat aaccagtaaa tacaatgaac atctacaaaa aacttctatt
      181 tatttataga tttcgtcagg caaatccaat tctctcttat caatcaaagt ctgataccac
      241 gaagcagatt ttttcaatgt tctgcttcgt tcatcgttca attcgattcg aactaatcca
      301 tatcgattct taaatgcatt cattggtgac acacagtcag tgaatgccca taacatatag
      361 ccttcgcaat ttgcgccttc ttctgttgct ttgatcaaat aacgcaaatg ctgtccgata
      421 aattcgatac ggtaatcatc ttgcacgatt cctttctcat ccatataccg ttcttcatgt
      481 tctcttccca taccgttttc tgttatcagc caagggatat tatgataatt ttcttttaga
      541 tacatggcta tatcatacat gattttcgga tagatttccc atcccctgga attattcatt
      601 ttcttcccag gtaaatcaaa gacttcataa tatcgttctg gatggaaagg tgtctcttca
      661 ttccattgat aacgaggagc gcgaacgcgt ttaggataat attgattgat tccaaggaaa
      721 tctactgtat gatgctgaat cgtttctaaa tcctctgttg caggatcgaa ataaatcgat
      781 tgtttttgac acaactccag gaactcttct gtgtagtacc cttttaccat cggatcaaaa
      841 aatactcgat tataaaacaa atcatacata taagcagctt tttggtcttc tggagaatta
      901 gatcgagcat agaccatttc aggatttaaa atacagccaa tccttccacc ataatttccg
      961 ctgcggtaaa tctctactgc tttagccgtt gctaaaactt tatgataatt ccacatcatc
     1021 cacttttttg tgttttgttc atgcggccag cgaacagcat ccaaatagca acgcgtttgg
     1081 ggaacgattg gttcgttgaa tgtaaaccag ttttttaccc tatcaccgta acgatca
//
LOCUS       DACEDD010000186         1106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEDD010000186 DACEDD010000000
VERSION     DACEDD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.207"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..999
                     /locus_tag="IWO45_003161"
                     /pseudo
     CDS             1..999
                     /locus_tag="IWO45_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          391 a          199 c          222 g          294 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtc
//
LOCUS       DACEDD010000187         1095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEDD010000187 DACEDD010000000
VERSION     DACEDD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.208"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1095)
                     /locus_tag="IWO45_003162"
     CDS             complement(<1..>1095)
                     /locus_tag="IWO45_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAQ0334565.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRL
                     TDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATW
                     LWKGKEPLFFCSKIRNEVDKKLFQARVTSL"
BASE COUNT          295 a          230 c          194 g          376 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttatggtaca ttggtttagc
      361 agaggacagt tcacacattc ttttggattg gatttgtatt ctcggtaccc ttctcgagta
      421 accgttgaaa aagcgagcat ttgatcttgc ggacaaatat aacaatcaaa atactcgtca
      481 tagagaaact cattttttga taatagctcc tttttccctt tgggccttga ataaggcaaa
      541 caaggtcgaa ggttcaaatg agtcaaaaag tgaacaaact taggtgtctt atacccagca
      601 tcagcgacaa cacaattgat ttcaggaaaa agtctcttcg attgtttgac gagctgaaag
      661 gcgacttgac tgtcatgaat atttctagga gtgatgattg tgctaagaat aaatccattg
      721 tcgtcgcatg aagtatggac agaataagca aattgttttt ctcgttcgcc tttgacgtag
      781 tagccgcttt ccggatcagc tttacttatt tttctttctt tcagcttcac ttccttttct
      841 aagtccaaag taaaagggcg ttttccagcc tctattcgtt gacgattgat ctcatcttct
      901 aattcatcct gataggcttt cgcttcatca tgggtcattt ctttcgtaaa cttatgttta
      961 ttggcgttag ctttgatgtg ggtagaatcc agatacaaat tatcctctgt tacaaaacct
     1021 gctttaactg cttgttctaa aatataggca aaaatatcct caaacaccgt cgtttctcta
     1081 aatcggcgaa cataa
//
LOCUS       DACEDD010000188         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEDD010000188 DACEDD010000000
VERSION     DACEDD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.210"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO45_003163"
     CDS             complement(36..992)
                     /locus_tag="IWO45_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334566.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACEDD010000189         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEDD010000189 DACEDD010000000
VERSION     DACEDD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.211"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWO45_003164"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWO45_003164"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEDD010000190         1021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEDD010000190 DACEDD010000000
VERSION     DACEDD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.213"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            155..>1021
                     /locus_tag="IWO45_003165"
     CDS             155..>1021
                     /locus_tag="IWO45_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0334567.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNLD"
BASE COUNT          393 a          121 c          208 g          299 t
ORIGIN      
        1 tattttagtt gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta aagtattgaa
       61 aacccttaaa attggttgca cagaaaaacc ccatctgtta aagttataag tgaccaaaca
      121 aataactaaa tagatggggg tttcttttaa tattatgtgt cctaatagta gcatttattc
      181 agatgaaaaa tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga gaccatggag
      241 agaaaagaaa atcgctaatg ttgattactt tgaacttctg catattcttg aatttaaaaa
      301 ggctgaaaga gtaaaagatt gtgctgaaat attagagtat aaacaaaatc gtgaaacagg
      361 cgaaagaaag ttgtatcgag tgtggttttg taaatccagg ctttgtccaa tgtgcaactg
      421 gaggagagca atgaaacatg gcattcagtc acaaaaggtt gttgctgaag ttattaaaca
      481 aaagccaaca gttcgttggt tgtttctcac attaacagtt aaaaatgttt atgatggcga
      541 agaattaaat aagagtttgt cagatatggc tcaaggattt cgccgaatga tgcaatataa
      601 aaaaattaat aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa taaataataa
      661 agataattct tataatcagc acatgcatgt attggtatgt gtggaaccaa cttattttaa
      721 gaatacagaa aactacgtga atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa
      781 attagactat gatccaaatg taaaagttca aatgattcga ccgaaaaata aatataaatc
      841 ggatatacaa tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat
      901 gaccgatgat gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg
      961 taaaaggtta atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaaccttga
     1021 t
//
LOCUS       DACEDD010000191         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEDD010000191 DACEDD010000000
VERSION     DACEDD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.214"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            369..1001
                     /locus_tag="IWO45_003166"
                     /pseudo
     CDS             369..1001
                     /locus_tag="IWO45_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DACEDD010000192          991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEDD010000192 DACEDD010000000
VERSION     DACEDD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.215"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IWO45_003167"
     tRNA            complement(21..94)
                     /locus_tag="IWO45_003167"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IWO45_003168"
     tRNA            complement(108..181)
                     /locus_tag="IWO45_003168"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IWO45_003169"
     tRNA            complement(209..281)
                     /locus_tag="IWO45_003169"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IWO45_003170"
     tRNA            complement(301..374)
                     /locus_tag="IWO45_003170"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IWO45_003171"
     tRNA            complement(384..457)
                     /locus_tag="IWO45_003171"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IWO45_003172"
     tRNA            complement(468..553)
                     /locus_tag="IWO45_003172"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IWO45_003173"
     tRNA            complement(570..641)
                     /locus_tag="IWO45_003173"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IWO45_003174"
     tRNA            complement(653..725)
                     /locus_tag="IWO45_003174"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IWO45_003175"
     tRNA            complement(743..824)
                     /locus_tag="IWO45_003175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="IWO45_003176"
     tRNA            complement(832..904)
                     /locus_tag="IWO45_003176"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="IWO45_003177"
     tRNA            complement(908..980)
                     /locus_tag="IWO45_003177"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DACEDD010000193          976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEDD010000193 DACEDD010000000
VERSION     DACEDD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.216"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..876
                     /locus_tag="IWO45_003178"
     CDS             <1..876
                     /locus_tag="IWO45_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0334568.1"
                     /translation="ILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQ
                     PGYVQYGAGSYANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPKR"
BASE COUNT          338 a          182 c          197 g          259 t
ORIGIN      
        1 atcctattgc atgaaacagc aaacgaaact gcgacaggac gaaatgaagc gcagtacatg
       61 aaacgttctt ggtacaatgc ctatacagcg tatattgttg gcgatggtgg gatcgtttac
      121 caagttggac aacctggtta tgtacaatat ggcgctggtt catatgctaa tgcaaatagt
      181 cctgtgcaaa tcgaactgca acatacgcac gataaagtaa cgtttgaaaa gaactacaag
      241 gcatatgttg aattagcgag agattccgct aataaatacg gtattccact tacattagac
      301 acaccgtata atcaaccagg aatcaaatca catttatggg taactcaaaa catttggggc
      361 gatcacactg atccgtatgg atacttatca gaaatgggtg taagtaagga aaaactggct
      421 tacgatttag ctcatggatt tacagacgaa aacccaacga cttctgagaa caagcctgtc
      481 attgatccaa cccgagcagg tgcagcaaat ccaactttat cagatggaac gaaccattct
      541 cacattgatc agtttggaga aatcgaaaat gcgaacttac acgtcgctgg atggcacatc
      601 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
      661 cgagtaagag ctgatggaat ttatagaccg gatgtaaatc aagcttataa tacttctgga
      721 aatgttggtt atcatgtatc tttcaatatg cgtaattttc ctagtaagaa agtctatgta
      781 atgatgcggg caacgaatga tccagaggga aacactaaag gcggtgcgca agatttccat
      841 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag
      901 tacataacta tattgacaac tataaaaatt attcgataaa atagtgatgt tatcgcatat
      961 cttcactatc acccat
//
LOCUS       DACEDD010000194          966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEDD010000194 DACEDD010000000
VERSION     DACEDD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.217"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..966)
                     /locus_tag="IWO45_003179"
                     /pseudo
     CDS             complement(1..966)
                     /locus_tag="IWO45_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          247 a          198 c          178 g          343 t
ORIGIN      
        1 ttggacaaga ctttgccagt ttaatttctg ttttatccgt tcattattgt agtaatagat
       61 atagctatca atggctgatt ttaattcctc gaaacagtta taaactttcc cgtgaaaaat
      121 ctcttgtttt aataagccaa aaaaattctc cataagtgaa ttatctaagc agttcccttt
      181 ccgagacatg ctttgaaaga ttttgtactc cttcaatttg tttctataag ctttcatttg
      241 atacgcccaa ccttgatcta agtgaaacat acgacgatag ggacaatcgt tagtggtctg
      301 gacagcttcc tccaaacctt ccattaccgc taatgcattg ggcttttcag agatacgata
      361 ggataaaatc tcactattga acatatccat aaaaggatct agatacaatt tcttttgacg
      421 gataatgcct ttggcatccg cttcatggta tttaaattca gttgtatccg tcgtgatttt
      481 ctgatggcag atactggtgt agaaacgacg atggatccga tttttggcta ctgctccaac
      541 ttgtcctcga taagaattgt agcgacgtga tttacgtgtg tatgccgtta cttttattcc
      601 tagcttcttg atcaggcgct gaactttctt tttattgata gagaaaccgc ggtttcttag
      661 ttcattggtc atccgtaaac agccataatc cttgtgcttc tcccgaattt taagcatttc
      721 cgcttcaatc tcttgatctg gatttttacg gtccaatcgt ttttgccagt acataaaggt
      781 tgccttaggg aactttaaag tttctagaag ctcaactaat ttaaacggtc ctcggaggct
      841 tgcgatgatt cgtgcgattg cttcattcgt cgttgttggg cttcctgttt tcgcagcttc
      901 ctcaattctt ttaaaaaggc attttcaatt tgaagggaac ggactcgttt ctccaactcc
      961 tcgatg
//
LOCUS       DACEDD010000195          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEDD010000195 DACEDD010000000
VERSION     DACEDD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.218"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IWO45_003180"
     CDS             <1..355
                     /locus_tag="IWO45_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334569.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="IWO45_003181"
                     /pseudo
     CDS             615..910
                     /locus_tag="IWO45_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DACEDD010000196          891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEDD010000196 DACEDD010000000
VERSION     DACEDD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.219"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>891
                     /locus_tag="IWO45_003182"
     CDS             <1..>891
                     /locus_tag="IWO45_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ0334570.1"
                     /translation="FIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSH
                     ASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQL
                     TKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKN
                     RANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDP
                     DLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRV
                     SYGFKSFENMRIRIFLINQL"
BASE COUNT          299 a          174 c          168 g          250 t
ORIGIN      
        1 ttttattgcg aaaaattgtc aggtatcttt gaccaccgtt attcgtacgt tgaaagagtt
       61 taaaagctac ttaccaaagc aatctaagag gattctcccc agagtattga tggttgatga
      121 atttcgttcg cacgcttcca tagaagataa aatgagcttt atttgcgcag atggcgaaac
      181 aggacaatta attgatgtct tgcctacgcg taaattgcct cgattaacaa gctatttctt
      241 aggctgcgct aatcctgaag aagtaaaatt tttagtcaca gacatgaacg ccgcctactt
      301 ccagcttacc aaacgtattc tgccaaatgc gaaagtagtg attgatcggt ttcatattat
      361 caaacacatg aatcaagcct ttaatgagtt gcgtatccgt gaaatgaatg aacttcgtaa
      421 agtaggacag aaaagtcagg cagaaaaact gaaaaagaac tggcgctttt tgctaaaaaa
      481 tcgtgcaaac atcaaccatt atgaatacaa aacatggaaa agttttcgag caccaaaata
      541 cccatttctt acggaagcga tgatgattga tcgattgctt ggcttttcta cgtccctgaa
      601 ggaggcgtat ccctattttc atgaattagt tgaagccttt cgagacaaag accctgactt
      661 atttttctcc ttattggcag aacttcccga aacgttagat gacggctttc gggaaaagct
      721 ccaaaacctt ctgacctatg aagaaggcat caccaacgcg atgatctatc cttacaccaa
      781 tggaaaaata gaagcgaaga atacccatat aaagacaatg aaacgagtat cctacggatt
      841 taaatcattt gagaacatga gaattagaat ctttttgatc aatcaattaa t
//
LOCUS       DACEDD010000197          879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEDD010000197 DACEDD010000000
VERSION     DACEDD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.220"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            486..>879
                     /locus_tag="IWO45_003183"
     CDS             486..>879
                     /locus_tag="IWO45_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0334571.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQ"
BASE COUNT          320 a          132 c          215 g          212 t
ORIGIN      
        1 ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg tacaaacgta ctgacgaact
       61 agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg gagagacagt gagcaaaaaa
      121 tcactcattg tgctaacttg tggttgagta aaagtcgttg ttatgaaaga ccatgctgcg
      181 ttacaggcgt aggagaagtt agggggcaaa agctagcatc taagttaagg catgtggata
      241 gatataaggg aacaggggaa gccatcaaca tggagagtgc atactcgatg aagtggtagt
      301 aagaaatatg tgaatgacaa ataacttact ttagtgatgg tgaaagtaga gtcacgactg
      361 atgaaggttc tgtaatggaa ctggaggaac aggctcgatt cgtctgtaaa agtcatgaaa
      421 cccgttcaac catgggattc tcgtatgact aaaaaggtca cgagccaatt ggagcgtgat
      481 aaaccatgac aaaagaaaca agtccaaaag ataggaagtt gcggcatgca gaatattatg
      541 gcatgacgga aatcttcgat aagatatact ccaaaagttt gaagggagat aatttcaaga
      601 atctaatgca gattatcatt tctgataaca acattgtcct agcatatcgt aatatcaaga
      661 gaaatcacgg tagtgtgaca agaggagaag atggagtaac gattaaagat attgaaaatc
      721 tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt cgcacattat aaccctcgga
      781 aagtaaggcg tgtagaaatt cctaaatata atggtaaatt aagaccgttg ggtattccat
      841 caatatggga tagaatcgcg cagcaatgca tcttacaag
//
LOCUS       DACEDD010000198          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEDD010000198 DACEDD010000000
VERSION     DACEDD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.221"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..861
                     /locus_tag="IWO45_003184"
     CDS             <1..861
                     /locus_tag="IWO45_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0334572.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DACEDD010000199          835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEDD010000199 DACEDD010000000
VERSION     DACEDD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.222"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>835
                     /locus_tag="IWO45_003185"
     CDS             <1..>835
                     /locus_tag="IWO45_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0334573.1"
                     /translation="KFNNHSYGFRPNRSAEHAIADCMARMNQSHMEYVVDVDIKGFFD
                     EVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVLPNGKLIYPTKGTPQGGILSPLLA
                     NINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDKGNIYHKLRKSTTLKEIYLVRYAD
                     DFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEKSKITNLSKEESEFLGFTMKLVTK
                     GNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNSKDTFIYIGVYNSMVIGIHNYFKI
                     AT"
BASE COUNT          339 a          133 c          154 g          209 t
ORIGIN      
        1 aaaattcaat aatcacagct atggtttccg cccaaataga tcagcagaac acgcaatagc
       61 agattgtatg gcccgaatga atcaaagtca catggaatat gtggtagatg tagatatcaa
      121 aggtttcttc gacgaagtca atcatactaa actcatgaga caaatctggt ctatgggtat
      181 acgagacaaa cagcttctct taatcattag gaaaatgtta agagcacccg tagtacttcc
      241 aaatgggaag ttgatatacc caacaaaagg aacaccacaa ggcggaatac ttagcccatt
      301 acttgcgaac atcaatctca acgaatttga ctggtggata gcaaatcagt gggaggaaaa
      361 agatgccaaa gagctaaaac atcattttag gaaaaatgga gcttgggata aaggtaatat
      421 ctatcacaag ctcagaaaga gtacaactct aaaagaaata tacctagtga gatacgcaga
      481 tgattttaaa attttctgta gaaattataa tgaagcgaaa aaagttttct atgcaactaa
      541 aatgtggtta caggagcgtc taaagttacc aatatctgaa gaaaagtcaa aaatcacaaa
      601 tctttcaaaa gaagaaagtg aatttttagg ctttacgatg aaattagtga ccaaaggtaa
      661 caaaaaggtt gttaatacgc atataggtac aaaagcacaa agcatgatta aagtgaaact
      721 aaaaaaacaa atcaaacgaa ttcaaaaaag tcctaatagc aaagatacct ttatctatat
      781 tggagtctat aacagtatgg ttattgggat tcataattac ttcaaaattg cgact
//
LOCUS       DACEDD010000200          835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEDD010000200 DACEDD010000000
VERSION     DACEDD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.223"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IWO45_003186"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IWO45_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0334574.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          237 a          159 c          140 g          299 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaa
//
LOCUS       DACEDD010000201          812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEDD010000201 DACEDD010000000
VERSION     DACEDD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.224"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..167
                     /locus_tag="IWO45_003187"
     tRNA            94..167
                     /locus_tag="IWO45_003187"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Asn,seq:gtt)
     gene            207..296
                     /locus_tag="IWO45_003188"
     tRNA            207..296
                     /locus_tag="IWO45_003188"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:241..243,aa:Ser,seq:gga)
     gene            311..382
                     /locus_tag="IWO45_003189"
     tRNA            311..382
                     /locus_tag="IWO45_003189"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Glu,seq:ttc)
     gene            398..470
                     /locus_tag="IWO45_003190"
     tRNA            398..470
                     /locus_tag="IWO45_003190"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:431..433,aa:Val,seq:tac)
     gene            480..552
                     /locus_tag="IWO45_003191"
     tRNA            480..552
                     /locus_tag="IWO45_003191"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:513..515,aa:Asp,seq:gtc)
     gene            588..663
                     /locus_tag="IWO45_003192"
     tRNA            588..663
                     /locus_tag="IWO45_003192"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:621..623,aa:Phe,seq:gaa)
     gene            665..745
                     /locus_tag="IWO45_003193"
     tRNA            665..745
                     /locus_tag="IWO45_003193"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:699..701,aa:Tyr,seq:gta)
BASE COUNT          185 a          182 c          223 g          222 t
ORIGIN      
        1 aaccatgccg aacacagaag ttaagcttct tagcgccgat tgtagtgagg ggttgcccct
       61 tgtgagagta ggacgtcgcc acgctgatgt tattccggca tagctcagtt ggtagtagcg
      121 catgactgtt aatcatgatg tcgtaggttc gagtcctact gccggagttc tcattttgag
      181 aaagagtatt gagcttcttt cactctggag agttgtccga gaggccgaag gagcatgatt
      241 ggaaatcatg tagatggtgc caaactgtct caagggttcg aatcccttac tctccgtagc
      301 aaatttacct ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg cggtaacacg
      361 ggttcgaatc ccgtacgggt cattttaatt gaatgttgga ggtttagctc agctgggaga
      421 gcatctgcct tacaagcaga gggtcagcgg ttcgatcccg ttaacctcca taatgaactg
      481 gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg ggttcgattc
      541 ccgtcggaac cgtcgttctt gttattcaat taataatttt aaaatatggc gcggtagctc
      601 agttggtaga gcaacggatt gaagctccgt gtgtcggcag ttcgattctg tcccgcgcca
      661 ccatggagga gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc
      721 agtggttcga atccactctc ctccatttta taggggcata gtttaaaggt agaacagcgg
      781 tctccaaaac cgttagtgtg ggttcaattc ct
//
LOCUS       DACEDD010000202          805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEDD010000202 DACEDD010000000
VERSION     DACEDD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.225"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWO45_003194"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWO45_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0334575.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          297 a          126 c          150 g          232 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtca
//
LOCUS       DACEDD010000203          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEDD010000203 DACEDD010000000
VERSION     DACEDD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.226"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            27..>793
                     /locus_tag="IWO45_003195"
     CDS             27..>793
                     /locus_tag="IWO45_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0334576.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT          284 a          148 c          136 g          225 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc
      541 aaaccgtatc caaagaagcg atttatattg ccattggtat acgagaagac ggcactaaag
      601 aagtactgag ttatgcgatt gctccaactg aatcaacata cgtttggaat gagctgctac
      661 aggatattaa ctccagagga gttcaagaag tcttgctttt tattacggac ggcttaaaag
      721 gcatgaaaga tactatccat caaatttatc ctaaagcaaa atatcagcat tgttgtatcc
      781 atgtatctcg taa
//
LOCUS       DACEDD010000204          789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEDD010000204 DACEDD010000000
VERSION     DACEDD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.227"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IWO45_003196"
     CDS             <1..782
                     /locus_tag="IWO45_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0334577.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          106 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgccc
//
LOCUS       DACEDD010000205          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEDD010000205 DACEDD010000000
VERSION     DACEDD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.228"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..751
                     /locus_tag="IWO45_003197"
                     /pseudo
     CDS             1..751
                     /locus_tag="IWO45_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          278 a          142 c          163 g          168 t
ORIGIN      
        1 caagtgaagc gctggatcaa tgcctatcaa gaatttggtg aagagggtct agtaagaaag
       61 cgacaaaaga aaaaatattc tgttcaattt aagctggatg cgatagagtt atatctaaca
      121 agtgagctgt cctatcgtga agttgccaac acacttaaca tgaacaaccc taatttgatc
      181 gctagttgga tgcgtcaatt ccgtgagggt gggatcgatg gactctcaaa aacgaaagga
      241 tgtccaccta tcttgtctaa gaaaaatgaa cccaaaaata agaaaaaatc tacaactaaa
      301 gcaacgtcta aagaacgtga acgcatcgag gagttggaga aacgagtccg ttcccttcaa
      361 attgaaaatg cctttttaaa agaattgagg aagctgcgaa aacaggaagc ccaacaacga
      421 cgaatgaagc aatcgcacga atcatcgcaa gcctccgagg accgtttaaa ttagttgagc
      481 ttctagaaac tttaaagttc cctaaggcaa tctttatgta ctggcaaaaa cgattggacc
      541 gtaaaaatcc agatcaagag attgaagcgg aaatgcttaa agttcgggag aagcacaagg
      601 attatggctg tttacggatg accaatgaac taagaaaccg cggtttctct atcaataaaa
      661 agaaagttca gcgcctgatc aagaagctag gaataaaagt aacgacatac acacgtaaat
      721 cacggcgcta caattcttat cgaggacaag t
//
LOCUS       DACEDD010000206          748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEDD010000206 DACEDD010000000
VERSION     DACEDD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.229"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..282)
                     /locus_tag="IWO45_003198"
     CDS             complement(<1..282)
                     /locus_tag="IWO45_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334578.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
     gene            complement(384..737)
                     /gene="tnpB"
                     /locus_tag="IWO45_003199"
     CDS             complement(384..737)
                     /gene="tnpB"
                     /locus_tag="IWO45_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0334579.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          217 a          152 c          132 g          247 t
ORIGIN      
        1 tttcgttcct acaactcgtt gatgtccttt tactggaata atggtggcag ctgcttctgg
       61 aacacttaca gaaatttctt catcactaaa aagattcaat tgcccattcg tttcctcaaa
      121 gagagtctct ttagagcgtc caaataattt gttggttaaa taattgacct tttcattcaa
      181 tagttggatt tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat tggttttatg
      241 ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc accaccttga
      301 atcgaactga ttttgttaca gaataccaca agaaacgcac aagaaatagg ggttcaacac
      361 attttttgtg ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga
      421 ggttgatcga tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga
      481 acttcgtctt tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga
      541 agaaaaccat caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat
      601 aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga
      661 ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga
      721 agtaaatcat aaagcattgg acaacagt
//
LOCUS       DACEDD010000207          715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEDD010000207 DACEDD010000000
VERSION     DACEDD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.230"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..>715
                     /locus_tag="IWO45_003200"
     CDS             7..>715
                     /locus_tag="IWO45_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0334580.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          250 a          126 c          134 g          205 t
ORIGIN      
        1 gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga gtcttatttc
       61 aaaatgaatc aatctgttgc taaaactgcc cattgcttga atcgttcaag acaaacgatc
      121 cataaagtat atctgttctt caagcaagga aaatcagcct tagaatatta tcaacagtat
      181 aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt tacccgagga acaatcagaa
      241 tatattcaaa gaaaggttgt tcaaggatgg acacccgatg tgattgttgg tcgtgcagcg
      301 tttcctatta gttgttctgc tcgtaccatt tatcgtatgt tcaaaaaggg gctatttgat
      361 tcttctgact taccgatgaa aggcaagcgt aaaccgaatg gacatcaaga aagacgtgga
      421 aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg attatagcca attctcaaat
      481 gagtttggtc accttgaagg tgacactatc gtaggtctga aacataaaag tgctgtaatt
      541 accttagttg aacgattatc aaaagttatc atcacattga aaccgtgtgg tagacaagcg
      601 attgatattg aaaaaaaatt aaatcaatgg tttgaatctg taccgaaaaa cctattcaaa
      661 tccatcactt ttgattgtgg aaaggaattt tcaaattgga aacagatcag taatg
//
LOCUS       DACEDD010000208          711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEDD010000208 DACEDD010000000
VERSION     DACEDD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.231"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..602
                     /locus_tag="IWO45_003201"
     CDS             <1..602
                     /locus_tag="IWO45_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0334581.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          181 g          190 t
ORIGIN      
        1 gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg
       61 cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga
      121 tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc
      181 atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct
      241 gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc gaaaaacgct
      301 tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc gaagcctcct
      361 atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg atcatttttg
      421 gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca tttgcaaaat
      481 tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc ccagcattag
      541 aagacaacgc tggaacgctc ggttgtttgg cattggctcg agaagcggtc ttacattcat
      601 aaacgaaaaa aggctcttcg acatttagtg ttggtagaga aagatacaac tgaaaattta
      661 gtcataacca gttaggcaga gtgcttaact ggtttttgtt ttacttggaa g
//
LOCUS       DACEDD010000209          710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEDD010000209 DACEDD010000000
VERSION     DACEDD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.232"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..>710)
                     /locus_tag="IWO45_003202"
     CDS             complement(109..>710)
                     /locus_tag="IWO45_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ0334582.1"
                     /translation="KGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWHY
                     ICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHE
                     ILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWN
                     YLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          199 a          164 c          131 g          216 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa
//
LOCUS       DACEDD010000210          693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEDD010000210 DACEDD010000000
VERSION     DACEDD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.233"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..605
                     /locus_tag="IWO45_003203"
     CDS             <1..605
                     /locus_tag="IWO45_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0334583.1"
                     /translation="IARELRPSFEIRLREQGISKEGQYGKYEGYKPYLRSGQIRYIRG
                     FPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTVEEWKISWLCSNPVLSKRAT
                     IEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYHESKSDSYENLVIVTEEVHR
                     VIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEIS"
BASE COUNT          275 a          106 c          152 g          160 t
ORIGIN      
        1 aaattgcaag agaactcaga ccaagttttg aaatacgact acgagaacaa ggaatctcaa
       61 aagaaggaca atacggaaag tacgaaggat acaagcctta cctacggtca ggacaaatac
      121 gatatataag agggtttcca atattaccag tagggtatat tcaacacaga catccgatgt
      181 gtaaaaaaag aaatattaat aaatacaccg aagaaggtag ggaagccatt cataagaagc
      241 aacaaaccgt agaagaatgg aagatatcgt ggttgtgtag caatccagtg cttagtaaga
      301 gagcaacgat tgaatttgcg gacaatcgaa tttcaaaatt catcgctcaa aagggaagat
      361 gtgcagtaac aggtgaggag cttatcttat ctgaaatgca ttgtcatcat attatccctt
      421 atcatgaaag caaaagtgat agctatgaaa acctagtgat tgttactgag gaggttcatc
      481 gggtaataca cgctacacaa agtgaaacga ttgaggaact tctaaaatat ctaaaattaa
      541 atccaaaaca aaaagaaaaa ctcaatgagt tgagattaaa agttggtaac gaagaaatat
      601 cttaataaat atttgtagga cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt
      661 atgcggtgaa agtcgcacgt acggtgtgga ctg
//
LOCUS       DACEDD010000211          670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEDD010000211 DACEDD010000000
VERSION     DACEDD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.234"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            154..417
                     /locus_tag="IWO45_003204"
     CDS             154..417
                     /locus_tag="IWO45_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0334584.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="IWO45_003205"
     CDS             417..>670
                     /locus_tag="IWO45_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0334585.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DACEDD010000212          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEDD010000212 DACEDD010000000
VERSION     DACEDD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.235"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(318..581)
                     /locus_tag="IWO45_003206"
     CDS             complement(318..581)
                     /locus_tag="IWO45_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ0334586.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACEDD010000213          550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEDD010000213 DACEDD010000000
VERSION     DACEDD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.236"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>550
                     /locus_tag="IWO45_003207"
     CDS             <1..>550
                     /locus_tag="IWO45_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334587.1"
                     /translation="YPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIE
                     YSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVI
                     LQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDP
                     LLQYYVDSTTHEIILSFLGKVQM"
BASE COUNT          197 a           88 c          110 g          155 t
ORIGIN      
        1 gtaccctgtt tatcatggaa gcgcaaaaag caacataggg attgagcagc ttatagaagt
       61 gataacgaat aaattttatt catcaacata cagaaagaag tctgaacttt gcggaaatgt
      121 cttcaaaatt gaatattcgg aagaaagaca acgtcttgca tatgtacgcc tttatggcgg
      181 aatcctgcat ttgcgggatt cggttagaat atcggaaaag gaaaaaataa aaattacaga
      241 aatgtatact tcaataaatg gtgaattatg taaaattgat aaggcttatt ccggggaaat
      301 tgttattttg caaaatgagt ttttgaagct aaatagtgtt cttggagata caaagctatt
      361 gccacagaga gagagaattg aaaatccgct ccctctgctg caaacaactg ttgaaccgag
      421 caaacctcaa caaagggaaa tgttacttga tgcactttta gaaatctccg acagtgaccc
      481 gcttctacaa tattatgtgg attctacgac acatgaaatt atactttctt tcttagggaa
      541 agtacaaatg
//
LOCUS       DACEDD010000214          542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEDD010000214 DACEDD010000000
VERSION     DACEDD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.237"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>542)
                     /locus_tag="IWO45_003208"
     CDS             complement(<1..>542)
                     /locus_tag="IWO45_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0334588.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELE"
BASE COUNT          147 a          116 c           84 g          195 t
ORIGIN      
        1 tgttcgagtt ccaatgcttc taatgattta ccggacatat atttctctaa aaggtcatcg
       61 tttccctcta ttaccgtatc ccattgttca gattcggtaa agttcgtcac acacatatta
      121 ggatacagtt ctaccttctg tttgattaca atttcggcag aaagtttctc tttaatatcc
      181 tgataaaccg ttgataaatc aattccattt tggtcaatct tattgataaa aaagattgtg
      241 ggaatcccca ttttcctaag tgcatgaaat aatatacgag tttgtgcttg tacgccatct
      301 tttgcagaaa tcagtagaat tgccccatct aaaactgata atgaacgata tacttctgct
      361 aagaaatcca tatgtcctgg cgtgtctatg atgttcacct tcgtattttc ccactgaaaa
      421 gaggttattc ctgtctgaat tgtaattcct ctctgacgtt ctaaaagcgt attatccgtc
      481 ctcgttgtac ctttgtccac gcttcctaat tctgtaatcg ctccactgtt atataataag
      541 ct
//
LOCUS       DACEDD010000215          540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEDD010000215 DACEDD010000000
VERSION     DACEDD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.238"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(58..360)
                     /locus_tag="IWO45_003209"
     CDS             complement(58..360)
                     /locus_tag="IWO45_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334589.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          185 a           93 c           75 g          187 t
ORIGIN      
        1 cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta
       61 atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc
      121 gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat
      181 tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa
      241 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
      301 gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat
      361 aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat
      421 tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg
      481 ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
//
LOCUS       DACEDD010000216          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEDD010000216 DACEDD010000000
VERSION     DACEDD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.239"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..>536
                     /locus_tag="IWO45_003210"
     CDS             51..>536
                     /locus_tag="IWO45_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0334590.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YL"
BASE COUNT          194 a           84 c          108 g          150 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctg
//
LOCUS       DACEDD010000217          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEDD010000217 DACEDD010000000
VERSION     DACEDD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.240"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWO45_003211"
     CDS             complement(<1..132)
                     /locus_tag="IWO45_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0334591.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACEDD010000218          504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEDD010000218 DACEDD010000000
VERSION     DACEDD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.241"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          226 a           70 c           81 g          127 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actc
//
LOCUS       DACEDD010000219          500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEDD010000219 DACEDD010000000
VERSION     DACEDD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.242"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..453
                     /locus_tag="IWO45_003212"
     CDS             <1..453
                     /locus_tag="IWO45_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAQ0334592.1"
                     /translation="KNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYS
                     GLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNV
                     FKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
BASE COUNT          212 a           57 c          105 g          126 t
ORIGIN      
        1 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
       61 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
      121 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
      181 cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt taaagcgtga tgtgagttta
      241 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
      301 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
      361 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
      421 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
      481 tttttttgat ggttttgaac
//
LOCUS       DACEDD010000220          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEDD010000220 DACEDD010000000
VERSION     DACEDD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.243"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..411)
                     /locus_tag="IWO45_003213"
     CDS             complement(<1..411)
                     /locus_tag="IWO45_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334593.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPF"
BASE COUNT          182 a           78 c           62 g          163 t
ORIGIN      
        1 aaacggcttt ccaaatgatg aaatacctaa atctaactta ttaagaaagt tgaaattttt
       61 atattcagga aaccatttat taatttcatg tgcagcacgt aatgcattcc ctggagttgt
      121 caaagagaaa attaattctg ctaagcttat tattgaaaat actgataact tataattata
      181 tcgtccattg acaatcaaat ataaactaac tatacttgtt agaacaaaaa aacaggcgtt
      241 gacttgttct tgattagtta aaaatatcaa taaaggaata cttacccaat atatttttct
      301 atttatttct tctcctctta agagttgaaa aaacggataa aaagttagta aactaaatgc
      361 tactggccac tgatagttca aggtggttgc tctccaaccg gtttccccca ttactgtcaa
      421 tggtattaaa cagtatatag agcaaaccaa taataaattt ttaacattta ttttctcaaa
      481 aacat
//
LOCUS       DACEDD010000221          467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEDD010000221 DACEDD010000000
VERSION     DACEDD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.244"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>467
                     /locus_tag="IWO45_003214"
     CDS             <1..>467
                     /locus_tag="IWO45_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0334594.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSV"
BASE COUNT          160 a           90 c           95 g          122 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtg
//
LOCUS       DACEDD010000222          466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEDD010000222 DACEDD010000000
VERSION     DACEDD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.245"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IWO45_003215"
     CDS             <1..411
                     /locus_tag="IWO45_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334595.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT          148 a           76 c           88 g          154 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atattt
//
LOCUS       DACEDD010000223          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEDD010000223 DACEDD010000000
VERSION     DACEDD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.246"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            237..>462
                     /locus_tag="IWO45_003216"
     CDS             237..>462
                     /locus_tag="IWO45_003216"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734812.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0334596.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLF"
BASE COUNT          157 a           86 c           59 g          160 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt tt
//
LOCUS       DACEDD010000224          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEDD010000224 DACEDD010000000
VERSION     DACEDD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.247"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IWO45_003217"
     tRNA            complement(20..91)
                     /locus_tag="IWO45_003217"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IWO45_003218"
     tRNA            complement(113..200)
                     /locus_tag="IWO45_003218"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IWO45_003219"
     tRNA            complement(225..298)
                     /locus_tag="IWO45_003219"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IWO45_003220"
     tRNA            complement(312..382)
                     /locus_tag="IWO45_003220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IWO45_003221"
     tRNA            complement(384..459)
                     /locus_tag="IWO45_003221"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACEDD010000225          430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEDD010000225 DACEDD010000000
VERSION     DACEDD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.248"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>430
                     /locus_tag="IWO45_003222"
     CDS             <1..>430
                     /locus_tag="IWO45_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0334597.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          145 a           81 c           74 g          130 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact
      301 aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg
      361 ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt
      421 agccataaaa
//
LOCUS       DACEDD010000226          416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEDD010000226 DACEDD010000000
VERSION     DACEDD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.249"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>416
                     /locus_tag="IWO45_003223"
     CDS             <1..>416
                     /locus_tag="IWO45_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334598.1"
                     /translation="FVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIYNYHGW
                     EGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEEGGASS
                     LESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          163 a           54 c           82 g          117 t
ORIGIN      
        1 atttgttttt aagaaaaaag tagtttcaga ctatttaaat aacgaaggcg gctacaaata
       61 tcttgcacat aaatatcaaa taaatcgtac actggttaga cattgggtaa ggatttataa
      121 ctatcatggt tgggaaggct tggttggagg tggcaaaagc tacactacaa aatttaaact
      181 tgatgttata gaatatatgg aaacaaatgg tctttctatc caagaaactg ctaaaaaatt
      241 taatatcggt tcaaatagaa ctctaagtaa atggatagag caatatgaag aaggcggtgc
      301 ttcttcactt gagagccaaa aaaggggcag aaaaattagt atgaattcca agctaaacat
      361 tcctaaaaaa cttaaagatg agtctcttga agaagaagtt attcgtttaa gagcag
//
LOCUS       DACEDD010000227          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEDD010000227 DACEDD010000000
VERSION     DACEDD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.250"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IWO45_003224"
     CDS             <1..390
                     /locus_tag="IWO45_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0334599.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          115 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatt
//
LOCUS       DACEDD010000228          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEDD010000228 DACEDD010000000
VERSION     DACEDD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.251"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWO45_003225"
     CDS             complement(<1..339)
                     /locus_tag="IWO45_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334600.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACEDD010000229          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEDD010000229 DACEDD010000000
VERSION     DACEDD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.252"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          164 a           50 c           38 g          129 t
ORIGIN      
        1 aaacaaaaat aaataaagtc ggatttttct gataattcaa cgcaaaaaaa cgaacaattt
       61 ttcaataaat cagtcttcta aaatagataa cctaaaaaaa acgaacaaac aaataaaatg
      121 actgtccttc attcactaaa catattttta ttttttatat tgttaaaaat tcacaattat
      181 tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt tttaacggca
      241 tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt
      301 cccatattgt gcaaaaaaat acaaggtgga ggctctacta tgattgaaga atatattgag
      361 aaaaatattt taagacagct g
//
LOCUS       DACEDD010000230          376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEDD010000230 DACEDD010000000
VERSION     DACEDD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.253"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            32..303
                     /locus_tag="IWO45_003226"
                     /pseudo
     CDS             32..303
                     /locus_tag="IWO45_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          137 a           54 c           70 g          115 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttaaatcta tattttgata tacaaattac
       61 agcttcagat actaagttgc ataaaccttt tccggacccc atacaaaagg caattgatga
      121 attgggcata aaaaataaga aacgatttat attggtgatt cattatatga catgcaaagt
      181 gcgaaagctt gtgggatagc atttggttta gctaaatggg gagccaaaaa tatagaaaat
      241 tttcaaagtg tagatataat tattgattct cctcaagaaa tacttgattt gttagaaaaa
      301 tagacaaaga gcgtgagata aggatagggt ctcacgctct ttgtttttcg ccatttaaag
      361 attgaaattt ttgcca
//
LOCUS       DACEDD010000231          372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEDD010000231 DACEDD010000000
VERSION     DACEDD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.254"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>372
                     /locus_tag="IWO45_003227"
     CDS             82..>372
                     /locus_tag="IWO45_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0334601.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          132 a           61 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cc
//
LOCUS       DACEDD010000232          332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEDD010000232 DACEDD010000000
VERSION     DACEDD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.255"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>332
                     /locus_tag="IWO45_003228"
     CDS             <1..>332
                     /locus_tag="IWO45_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0334602.1"
                     /translation="EFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE
                     TALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSN
                     GILRRNGL"
BASE COUNT          101 a           67 c           60 g          104 t
ORIGIN      
        1 ctgagtttgg tcaccttgaa ggtgatacga tcgttggcat tcatcataaa agtgccgtca
       61 ttactttagt tgaaagatta tctaaagtca ttatcacgat taaacccaac ggccgtaagg
      121 cattagatat tgaaactgcc cttaatcaat ggttttctcg cttccctaaa aacttcttta
      181 aatctattac gtttgactgt ggaaaagaat tttctaactg gaaagccatt agtaaccaac
      241 atgatattga tatatatttt gcggaccctg gaacaccttc tcaacgccca ttaaacgaga
      301 attctaacgg gattctgcgt cgtaatggac tg
//
LOCUS       DACEDD010000233          319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEDD010000233 DACEDD010000000
VERSION     DACEDD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.256"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            76..>319
                     /locus_tag="IWO45_003229"
     CDS             76..>319
                     /locus_tag="IWO45_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0334603.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
BASE COUNT          134 a           51 c           54 g           80 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaag
//
LOCUS       DACEDD010000234          311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.257, whole genome shotgun sequence.
ACCESSION   DACEDD010000234 DACEDD010000000
VERSION     DACEDD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.257"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(40..>311)
                     /locus_tag="IWO45_003230"
     rRNA            complement(40..>311)
                     /locus_tag="IWO45_003230"
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           83 c           77 g           74 t
ORIGIN      
        1 ctgaacaaag taaaaacaaa ttgtgtagtc tccgtaatat tccttagaaa ggaggtgatc
       61 cagccgcacc ttccgatacg gctaccttgt tacgacttca ccccaatcat ctatcccacc
      121 ttaggcggct ggctccaaaa ggttacctca ccgacttcgg gtgttacaaa ctctcgtggt
      181 gtgacgggcg gtgtgtacaa ggcccgggaa cgtattcacc gcggcgtgct gatccgcgat
      241 tactagcgat tccggcttca tgcaggcgag ttgcagcctg caatccgaac tgagagaagc
      301 tttaagagat t
//
LOCUS       DACEDD010000235          309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.258, whole genome shotgun sequence.
ACCESSION   DACEDD010000235 DACEDD010000000
VERSION     DACEDD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.258"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWO45_003231"
     CDS             complement(<1..302)
                     /locus_tag="IWO45_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0334604.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           97 a           45 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatat
//
LOCUS       DACEDD010000236          299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.259, whole genome shotgun sequence.
ACCESSION   DACEDD010000236 DACEDD010000000
VERSION     DACEDD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.259"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>299
                     /locus_tag="IWO45_003232"
     CDS             <1..>299
                     /locus_tag="IWO45_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0334605.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DACEDD010000237          298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.260, whole genome shotgun sequence.
ACCESSION   DACEDD010000237 DACEDD010000000
VERSION     DACEDD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.260"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            45..>298
                     /locus_tag="IWO45_003233"
     CDS             45..>298
                     /locus_tag="IWO45_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334606.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT          114 a           41 c           56 g           87 t
ORIGIN      
        1 attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa
       61 atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg
      121 agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga
      181 ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag
      241 aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatatta
//
LOCUS       DACEDD010000238          295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEDD010000238 DACEDD010000000
VERSION     DACEDD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.denovo.261"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..295
                     /locus_tag="IWO45_003234"
                     /pseudo
     CDS             1..295
                     /locus_tag="IWO45_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          115 a           59 c           58 g           63 t
ORIGIN      
        1 aaacaacaaa tcgtcgatct ctatctcgct ggtaagcctc gcgcagaaat tattcgagag
       61 tatgagctta cgccttcttc tttcgataaa tggatgaagc aagcacaatc aacgggctca
      121 ttcaaagaaa gagacaactt aacaccagaa caagcagaat tgatcgcact aagaaagaaa
      181 aataagcaac tcgaaatgga gaatgatatt ttaaagcaag cggcgctgat attcggacga
      241 aaagacaagt aattgatgcc aacaagcata aatattccat atcagcgatg tgcaa
//
LOCUS       DACEDD010000239         5377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.116, whole genome shotgun sequence.
ACCESSION   DACEDD010000239 DACEDD010000000
VERSION     DACEDD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.116"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..259)
                     /locus_tag="IWO45_003235"
     CDS             complement(<1..259)
                     /locus_tag="IWO45_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0334607.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQR"
     gene            404..589
                     /locus_tag="IWO45_003236"
     CDS             404..589
                     /locus_tag="IWO45_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0334608.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1101..1319
                     /locus_tag="IWO45_003237"
     CDS             1101..1319
                     /locus_tag="IWO45_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334609.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            1532..2191
                     /gene="vanR"
                     /locus_tag="IWO45_003238"
     CDS             1532..2191
                     /gene="vanR"
                     /locus_tag="IWO45_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0334610.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2194..3537
                     /gene="vanS"
                     /locus_tag="IWO45_003239"
     CDS             2194..3537
                     /gene="vanS"
                     /locus_tag="IWO45_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0334611.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            3708..4514
                     /gene="vanY"
                     /locus_tag="IWO45_003240"
     CDS             3708..4514
                     /gene="vanY"
                     /locus_tag="IWO45_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0334612.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4527..5363
                     /gene="vanW"
                     /locus_tag="IWO45_003241"
                     /pseudo
     CDS             4527..5363
                     /gene="vanW"
                     /locus_tag="IWO45_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         1592 a         1102 c         1332 g         1351 t
ORIGIN      
        1 ccctttgccg cagttcgctt tcctcccgca tgatctccgg gttaaagtag cgttccacag
       61 gaagcccgca ggctttaata agctgtatga caaccggcag actgggaatg gccccgtcgt
      121 tttcgatgtt ggcaagatag cgccagtcaa tatttatttt ctctgccatt gctttgcgcg
      181 aaacgcgctt tgcttgccgg gccgctttca catccgcgcc gaaagtttca aagccgggac
      241 aatcttcaat attcgccata tcgcacctcc cacttacatt gtatatttca tactctgccc
      301 gtggaatgtt ggtatatgca cctttcttaa agtttatatt tcacattttg tttcttttat
      361 tcttcgagca aaagaattat aatgtaccct gtggaggtgt actatggact acatgacgtt
      421 gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg ataaactact attgtgctgc
      481 gggacgcatc cccggcgctg taaagatggc gacgatctgg ctcattccca aaactgcgga
      541 aaagccgatt gacaggcgta caaagaaagg aaaggcggtt actgaatagt ataatgaaac
      601 cggcgcattt tggatatggt tttgaagaga gagaaagcat ggtattttgt cacggaaaaa
      661 ctataagatg atagatggca ggctgctcca gaccgataaa aagtattctc atctgaaaca
      721 gaaagaaaag atttacacat ggatgtatga agaagcgaaa ctttatcatg acagaaatgg
      781 aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc agccgtatat ggtagaattg
      841 aaaaggccgg catctggatt ctttatggtg aagtctccag acattggata actgctggtc
      901 taatgcgcct ttgagtatgg ggtgattgat tttaataccg gggaggtacg gctttttgag
      961 gaaaatattc tgggatttac atttggcgat tattacattt cctgtaactg caaggacacg
     1021 gatgatgaag atatgaggaa tatagacttg ctgattttct actgtcttga acggtattag
     1081 gactgaatct gctgcgcgga gtgcggcaag agcgaccgga gggagtacac acaggccctg
     1141 cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg cattttgctg
     1201 aggcttgggg tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa gtggtggatc
     1261 agaatatccg taaaggatat tctgatccat tgcttgccag tattatcggg atgttttgac
     1321 aaccgctgta atcgaaacat cccaaacatt aagaaggtgt taattattat tatcttgatt
     1381 taattcgttt aaaccaataa tatagataca tacaccatga aaagaagcga gtatcccaat
     1441 gacggatgat taaaatccga aaagaaatcg ctttgaaact acagggaaac tacagactgt
     1501 tatgttatct tcttaaaatg gagggatttt tatgtcgata cgaattctac ttgtcgagga
     1561 tgatgatcat atctgtaata cagtaagggc gtttctggct gaggcaggat atcaggtgga
     1621 tgcctgcaca gatggaaatg aggcatacac caagttttac gaaaacactt atcaactggt
     1681 tattcttgat attatgctgc ccggtatgaa cgggcatgaa cttttgcgtg aatttcgtgc
     1741 gaaaaatgat actcccattc tgatgatgac agccctgtcg gatgacgaaa accaaatccg
     1801 ggcgtttgat ctagaggcag acgactatgt aacaaagcca ttcaagatgc agattttact
     1861 aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg gcaaaggaaa tccgtgtcgg
     1921 caggctgaca cttctgccgg aggatttcac ggtactttgt gacggtacag agctgcccct
     1981 gacacgaaaa gaatttgaaa tccttttgct gctggtgcag aacaaaggca gaaccttaac
     2041 gcatgaaatc attttgtcac gtatatgggg atatgacttt gagagtgatg gcagcacagt
     2101 ccacactcat atcaaaaatt tgcgggccaa gctgccggaa aatatcatca aaaccattcg
     2161 cggtgtagga taccgattgg aggaatcata ataatggaaa gaaaagggat tttcattaag
     2221 gttttttcct atacgatcat tgtcctgcta ctgcttgtcg gtgtaacagc aacactgttt
     2281 gcacagcaat ttgtgtctta tttcagagtg atggaattac agcaaacagt aaaatcctat
     2341 cagccattgg tggaactgat tcagaatagc gataggcttg atattcaaga ggtggcaggg
     2401 ctgtttcact acaataacca atcctttgag ttttatattg aagataaaga gggaagcgta
     2461 ctctatgcca caccaaatgc caatacatca aatagtttta gacccgactt tctttatgtg
     2521 gtacatagag atgataatat ttcgattgtt gctcaaagca aggcaggtgt gggattgctt
     2581 tatcaagggc tgacaattcg gggaattgtt atgattgcga taatggttgt attcagcctt
     2641 ttatgcgcgt atatctttgc gcggcaaatg acaacgccga tcaaagcctt agcggacagt
     2701 gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc tggagcgaaa ggatgagctt
     2761 ggcgcactgg ctcatgatat gcattccatg tatgtcaggc tgaaagaaac catcgcaagg
     2821 ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa cacagcgata tttctttgcg
     2881 gcagcttctc atgagctaaa aacgcccatc gcggctacaa gcgttctgtt ggagggaatg
     2941 cttgaaaata tcggtgacta caaagatcat tctaagtatc tgcgcgaatg catcaaaatg
     3001 atggataggc agggcaaaat catttccgaa atactggagc ttgtcagcct gaatgatggg
     3061 agaatcgtac ccatagctga accgttggac atagggcgca cggttgccga gttgctgccc
     3121 gattttcaaa ccttggcaga ggcaaacaac cagcggttcg tcacagatat tccagccggg
     3181 caaattgtcc tgtccgatcc gaggctgctc caaaaggcac tatccaatgt catattgaat
     3241 gcagttcaga acacgccgca gggaggcgag gtacggatat ggagtgagcc tggtgctgaa
     3301 aaatgccgcc tttttgtttt gaacatgggc gttcacattg atgatactgc gcttccaagg
     3361 ctgttcaccc cattctatcg cattgatcag gcgcgaagca gaaaaagtgg gcgaaacggt
     3421 ttaggacttg ccatcgtaca aaaaacgctg gatgccatga gcctccagta tgcgctggaa
     3481 aacacctcgg atggcgtttt gttctggctg gatttaccgc tcacatcaac attgtaaata
     3541 tttaaaactt aaatgatttt gaccgacagg tataaccctg ccggtctttt tttgctgcta
     3601 caggaaaact acagactggc tacagggaaa gtacagatgc gcttgccata ataacaatcg
     3661 taccagccac aaatcgtagt tttattgcaa aggaggcatt cgatcaaatg gaaaaaagca
     3721 actatcattc caatgcggat catcacaaac ggcatatgaa acaatccgtg gaaaagcggg
     3781 cttttctatg tgcgttcatt atttcgttca cagtctgcac gctgtttttg gggcggagac
     3841 tggcttccgt attggaggca acacagatac cgcccatccc tgcaactcat acaggcagca
     3901 gcactgacgt agtggagaat ttggaggaaa acgctcttgc caccgccaaa gaacagggag
     3961 atgaacagga atggagcctg attttagtga acaggcagaa ccccatcccc gcacagtacg
     4021 atgtggagct tgagcaacta tcaaatggtg agcggataga tattcggatt tctccctatc
     4081 ttcaagattt gtttgatgcc gcaagaactg atggagttta cccgattgtc gcatccggat
     4141 accgaacaac agaaaaacag caagaaatta tggatgaaaa aattgccgaa tataaggcga
     4201 aaggctacac ctctgcacag gctaaagcgg aagcagaaac ttgggtggcc gtgccgggaa
     4261 cgagcgagca tcagcttggt cttgctgtgg atatcaatgc ggacggaatt cattcaacag
     4321 gcaacgaggt ttatagatgg ctggatgaaa acagctatcg ttttggtttt attcgtcgct
     4381 acccaccaga caagacagag ataaccggtg tgagcaacga gccgtggcat taccgatatg
     4441 ttggcatcga agctgccaca gagatgtaca accaaggggt ttgccttgag gaatatttaa
     4501 aaccagaaaa atgagaaaag gatgttatgg acagaaaaag attgacacag cgtttcccgt
     4561 tcctgcttcc aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg
     4621 acggctgtcg ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa
     4681 cggattgcgc attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg
     4741 tgttcaactt aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa
     4801 ccttttcttt ctggtggctg gtacgccatg cggacaaaga taccccctat aaagacggcc
     4861 ttacggtgac caatggtaaa ctcaccacca tgtcgggcgg cggtatgtgc cagatgagca
     4921 atttactatt ttggatgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg
     4981 aagtaaagga gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt
     5041 cagagggctg gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatgag
     5101 tgaccttaga tgatgagaaa atcatcggtc aggtgtccgc cgacaaagag ccccaagcat
     5161 tatacaaaat tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat
     5221 atgcccaggt taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc
     5281 tgctttatac aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagt
     5341 ctaatggtga taagtcaaga tgaaatttaa gattttc
//
LOCUS       DACEDD010000240         5155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.121, whole genome shotgun sequence.
ACCESSION   DACEDD010000240 DACEDD010000000
VERSION     DACEDD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.121"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..729
                     /locus_tag="IWO45_003242"
     CDS             <1..729
                     /locus_tag="IWO45_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0334613.1"
                     /translation="GFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAID
                     EIYHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFT
                     KRGVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYF
                     QKKLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWA
                     KRFFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(872..1813)
                     /locus_tag="IWO45_003243"
     CDS             complement(872..1813)
                     /locus_tag="IWO45_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0334614.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1939..3060
                     /locus_tag="IWO45_003244"
     CDS             1939..3060
                     /locus_tag="IWO45_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0334615.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3318..4820
                     /gene="lsa"
                     /locus_tag="IWO45_003245"
     CDS             3318..4820
                     /gene="lsa"
                     /locus_tag="IWO45_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0334616.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSQDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4887..>5155
                     /locus_tag="IWO45_003246"
     CDS             4887..>5155
                     /locus_tag="IWO45_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0334617.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDG"
BASE COUNT         1690 a          930 c         1107 g         1428 t
ORIGIN      
        1 ggatttgata tggagatggc tcgacgcgtg cgtccattta ccaatgcaaa aatcgtgtta
       61 tttttttgga accactttaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa
      121 ccggcaatcg atgagattta tcattttgat tttttagaag cgaaagaact tggactaaaa
      181 cataatagtt ccttttattc gaaacatatg gatttttctt ctagcgaacc aaagacggat
      241 ctattttttg gtgcaacaaa taacggcaga aaagaacggg cagaaaaata taagcaggaa
      301 tttacaaaaa gaggtgttac gaccaattac tttattcttc cgcttcgagg aaatgaacaa
      361 cctggatacc ttacttatga agaatatctg aaaaaaaccg gggaatcgag ggggattcta
      421 gaactgttgc gggaagggca gcaaggagtg actttgcgaa cgtttgaatc tatgtatttt
      481 caaaagaaac tagtgacaga taatcaagcc atagctcatt atcagtttta tcattctgaa
      541 aatatttttt tgcttcaaga acgtgattta gatgaattac cggaattttt gcatacgcca
      601 tttcaaccaa tcccatcaga actgttggaa ttttttgatg cagaaaattg ggcaaaacgt
      661 tttttcacta ctgactccgc tatttatgaa caatatgaat acgatctttc ctctgttaaa
      721 aataaataaa agtgccaacc aatcatagat gaaaataaac aagcgcaatt cttatggcaa
      781 catgagaatt gtgtttttct ttttgaactt tcatgggggt aaaaaaatga gaaccagctg
      841 ctgatccagc tgattctcat cagagagcct tttattctgc gccaaggatt ccttcttttg
      901 catggactaa ttgggtcaaa aaggcacaat atggtgtagc aatatcatat ttttgtcctt
      961 ttcgccaaac ggcaccattg atgtagtcga tttcagtcaa acgatggttt ttgatcaggt
     1021 cttggtacat agatgggtaa tgcaacccga tgttgtcagg atcataagta gaagcaatat
     1081 gttggtacac ttcttcttga tctaatgtga ttccttcttt tgctgcgata gaagcaaact
     1141 cagatacaat cgttcgaacc atgccctctg ccgctttttg cgcacctaat tcagcaatat
     1201 tacaatccaa tatcgtgcat aatccattta atgttccgtt tacacaagct tttcgccaaa
     1261 tggaataacg aacattatga ctatagattg gattcaattt tgcttcttga aagacttcta
     1321 caactttttt tgtaaatgct tcgccttctg gttccaagtt ctctagttcg atttctccat
     1381 cacctaataa tgtgactttt ccgggaccag ctagaccagc tgtccacatc gtgatcccaa
     1441 ataagatatt tttctttggt acatattttt ccagtacatc ttcatgacct aatccattca
     1501 gtaaacacaa aacataagta ttctcagtaa taatggattg gatcgaacga aacatatcat
     1561 ctaattgatt cgcttttgtc aaggctacga tcaaatccac gtgtttgttg ctttcgatga
     1621 tttcttctgg tgaatagatt ggcagttttg caactacttc tttgccatta aagtcagcaa
     1681 tcaatccatt tgtacgaatg gcttcaatgt gagcaggcca gcgatcgatc aagacgacct
     1741 catttccgct ttgatgcaac attaatccga ttcggctgcc cattgctccg gctccagcta
     1801 ttgcgatttt catgtttttt ctctccttta ttcaatgagt tcccttttag tatatagaaa
     1861 aacagaagaa aatagcattg ttttgtttga aaaaagtgaa aaaaagtgtc ttacttaaga
     1921 agaaagtgag ggatatagat ggatattttg gcggcaatcg attcatttaa aggaagtgct
     1981 tcttcatttg aattaaatca ggcagttcta aatggttggg aagaccgtaa aggaagaaaa
     2041 atcaatgtgc cgattgcaga tggcggagaa aatactgcag aagcaatcta ttacgcgctc
     2101 gggggaacct ggcgtacgat tgatgggatg gatctcttat ttcgtcagcg aaaaatcagt
     2161 tatttattga cgaactggca aggaaaaaat accgcagtca ttgaatctgc acaagtatta
     2221 ggattagatt tacttgataa accaacggat caaaccatcc gacttgcttc tagttatgcc
     2281 ttgggagagc ttatcaaaga tacactgctt caagaagtgg aacaaatcat tgtcacttta
     2341 ggaggaagcg gatgtagcga tggtggtcta ggattactcc aatctttggg tgcttcttta
     2401 gaagggtaca cagaaggaaa tcctttgtta tctactagaa aacttcattt ggatcgaagg
     2461 aacgttcttg tttctaaagt aaaagtgatc atcgctgcag acgtgaccaa cccatataca
     2521 ggtcaacaag gagctgcttg gcaatttgga agacaaaaag gaggaacgga tacgacgtta
     2581 gctttcttgg ataaacaagc tgaaaagatt gctcagcaga taaaagatgt ttgggaggtg
     2641 gatttagatg acatcccagg aagcggagca gcaggcggta ttggcggagc cctttttttg
     2701 ttaggagcga agatggtctc tggttttgag ttggtcagcc aaatcactgg attagaaaaa
     2761 cagatcgaac aagcggatgt catcattaca ggagaagggc ggattgatca gcaaacaatc
     2821 cacggaaaag ttccatatgg cgtagcagtg cttgcaaaaa aatatcgaaa accaataatc
     2881 gctatttgcg gaagtagaga ccgagagctg gaaggactct ttgattttct ccccattgtc
     2941 ttaagcattc aattaggacc aatcgattta gaggaagcaa tggaacacag gcaaacatta
     3001 gaaaaaacag aaatcttagg acagacactc agcagatttt tcaacattct cccgaagtag
     3061 ttgacaaaaa caatttcttt atgctatcgt aatttcaaat ataggacaaa gtgagggatc
     3121 cgtgcattga aaaattaagt cgattgatga aagctgcatt cagactgtac cttgtggaga
     3181 caagggagca gtgtgcagat tttcgggatt ggcttggttc tttcgatgta tgggtctttt
     3241 tgtgtgcccc aacccttgca tcctctttag ccagttctga aaataggact ggtctttttt
     3301 tacgaggtga tggaataatg tctaaaatcg aaataaaaaa tctgacattc ggctatgaca
     3361 gccaaggcac attattattt gaacaagcca atctaaactt tgacacacaa tggaaactag
     3421 gacttatcgg acgaaacggt cgaggaaaga caactttact gaatatcctg caaaacaaac
     3481 tcccttatca agggcaagtg atccatcagc aagcgtttgt ctatttcccg cagcaaacaa
     3541 aagataaaga gcgtttgacc tattatgtgt taaatgagat tatggatttt gagatatggg
     3601 aaattgaaag agagctccaa ctgatgcaaa cagatcctga aatcttatgg agagaattca
     3661 gcacactatc aggaggagag aagacaaaag tcctactggc actcttattt gtggatgaca
     3721 ctcatttccc gttaatcgat gaaccaacga atcatttgga tatctctggc agaaaacaag
     3781 tagcggctta cttgaaaaag aaaaaacaag gattcatcgt ggtgagtcag gatcggggat
     3841 ttatcgatga agtagtggac catgttttag caatcgaaaa gagccaactg gaactttatc
     3901 aagggaattt ctctatctat gaagaacaga aaaaacttcg tgatgaattt gaaatagcta
     3961 aaaatgaaaa attgaaaaaa gaagtcagca ggctaaagaa aacagcagct gaaaaagcag
     4021 aatggtctcg ttcccgagaa ggggacaaaa caaagaaaaa agtcggattc atcgatacgg
     4081 aatctaggcg agtgaataaa ggagcagtag gcgctgatgc tgcacggacg atgaaacgat
     4141 ctaaagcaat cgtgaatcgg atggaaaccc agatcagtga gaaagaaaaa ttattaaaag
     4201 atatcgagta tatcgatgct ttgacgatga atagccaagt gtctcatcat aaacggctgt
     4261 taagcgtaga aaatcttcaa ttagggtatg aaaatctgtt attcgagcca gttcatttta
     4321 cgatcgaacc tcatcagcgg atggcaattt caggtcctaa cggcgcggga aagtcatcca
     4381 ttatccatta tcttctgggg acattcaacg gcaaggttgt aggagaaaaa tcccagccca
     4441 aacatttgac cattagttac gtacgccaaa attatgaaga caatcgaggg acattggcgg
     4501 aattcgcgaa gaaaaaccga gtagactatc aagcattttt gaataatctc cgaaagcttg
     4561 ggatggaaag agatgttttt cataataaaa tcgaacagat gagtatggga caacggaaaa
     4621 aagtggaatt ggctaaatct ttatcccaac cagctgagct ctatatatgg gatgaaccat
     4681 tgaattattt ggatgtcttc aatcaagaac aattagaaca actgattttg aacgtgaaac
     4741 ctgctatgtt actagtggaa catgatcaaa cctttctaga taaagtatct actgagatta
     4801 tttctcttga gaaaatctaa accttgtaat ctcaaagaca acaaggtatg gttaaggaga
     4861 actttgtcaa cgaaggagag aatcacatgc tttatgtaac gagacacgga gaaacaactt
     4921 ggaacgcaca aggattagtt tgcgggcgag cagatgtgcc gctgactgaa aaaggacaga
     4981 tgcaggcaca ggcactagca gaaaaggtag tagatttgcc tgttccaatc acgaaaatca
     5041 tccattcccc tcttcaacga gcaagagata cagcacaagc agtagttgat cgtctcaatt
     5101 tgccgatgac cgctgatgag cgattggtag aaatggattt tggggattat gacgg
//
LOCUS       DACEDD010000241         5155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.122, whole genome shotgun sequence.
ACCESSION   DACEDD010000241 DACEDD010000000
VERSION     DACEDD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.122"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..729
                     /locus_tag="IWO45_003247"
     CDS             <1..729
                     /locus_tag="IWO45_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0334618.1"
                     /translation="GFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAID
                     EIYHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFT
                     KRGVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYF
                     QKKLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWA
                     KRFFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(872..1813)
                     /locus_tag="IWO45_003248"
     CDS             complement(872..1813)
                     /locus_tag="IWO45_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0334619.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1939..3060
                     /locus_tag="IWO45_003249"
     CDS             1939..3060
                     /locus_tag="IWO45_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0334620.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3318..4820
                     /gene="lsa"
                     /locus_tag="IWO45_003250"
     CDS             3318..4820
                     /gene="lsa"
                     /locus_tag="IWO45_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0334621.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4887..>5155
                     /locus_tag="IWO45_003251"
     CDS             4887..>5155
                     /locus_tag="IWO45_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0334622.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDG"
BASE COUNT         1690 a          930 c         1106 g         1429 t
ORIGIN      
        1 ggatttgata tggagatggc tcgacgcgtg cgtccattta ccaatgcaaa aatcgtgtta
       61 tttttttgga accactttaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa
      121 ccggcaatcg atgagattta tcattttgat tttttagaag cgaaagaact tggactaaaa
      181 cataatagtt ccttttattc gaaacatatg gatttttctt ctagcgaacc aaagacggat
      241 ctattttttg gtgcaacaaa taacggcaga aaagaacggg cagaaaaata taagcaggaa
      301 tttacaaaaa gaggtgttac gaccaattac tttattcttc cgcttcgagg aaatgaacaa
      361 cctggatacc ttacttatga agaatatctg aaaaaaaccg gggaatcgag ggggattcta
      421 gaactgttgc gggaagggca gcaaggagtg actttgcgaa cgtttgaatc tatgtatttt
      481 caaaagaaac tagtgacaga taatcaagcc atagctcatt atcagtttta tcattctgaa
      541 aatatttttt tgcttcaaga acgtgattta gatgaattac cggaattttt gcatacgcca
      601 tttcaaccaa tcccatcaga actgttggaa ttttttgatg cagaaaattg ggcaaaacgt
      661 tttttcacta ctgactccgc tatttatgaa caatatgaat acgatctttc ctctgttaaa
      721 aataaataaa agtgccaacc aatcatagat gaaaataaac aagcgcaatt cttatggcaa
      781 catgagaatt gtgtttttct ttttgaactt tcatgggggt aaaaaaatga gaaccagctg
      841 ctgatccagc tgattctcat cagagagcct tttattctgc gccaaggatt ccttcttttg
      901 catggactaa ttgggtcaaa aaggcacaat atggtgtagc aatatcatat ttttgtcctt
      961 ttcgccaaac ggcaccattg atgtagtcga tttcagtcaa acgatggttt ttgatcaggt
     1021 cttggtacat agatgggtaa tgcaacccga tgttgtcagg atcataagta gaagcaatat
     1081 gttggtacac ttcttcttga tctaatgtga ttccttcttt tgctgcgata gaagcaaact
     1141 cagatacaat cgttcgaacc atgccctctg ccgctttttg cgcacctaat tcagcaatat
     1201 tacaatccaa tatcgtgcat aatccattta atgttccgtt tacacaagct tttcgccaaa
     1261 tggaataacg aacattatga ctatagattg gattcaattt tgcttcttga aagacttcta
     1321 caactttttt tgtaaatgct tcgccttctg gttccaagtt ctctagttcg atttctccat
     1381 cacctaataa tgtgactttt ccgggaccag ctagaccagc tgtccacatc gtgatcccaa
     1441 ataagatatt tttctttggt acatattttt ccagtacatc ttcatgacct aatccattca
     1501 gtaaacacaa aacataagta ttctcagtaa taatggattg gatcgaacga aacatatcat
     1561 ctaattgatt cgcttttgtc aaggctacga tcaaatccac gtgtttgttg ctttcgatga
     1621 tttcttctgg tgaatagatt ggcagttttg caactacttc tttgccatta aagtcagcaa
     1681 tcaatccatt tgtacgaatg gcttcaatgt gagcaggcca gcgatcgatc aagacgacct
     1741 catttccgct ttgatgcaac attaatccga ttcggctgcc cattgctccg gctccagcta
     1801 ttgcgatttt catgtttttt ctctccttta ttcaatgagt tcccttttag tatatagaaa
     1861 aacagaagaa aatagcattg ttttgtttga aaaaagtgaa aaaaagtgtc ttacttaaga
     1921 agaaagtgag ggatatagat ggatattttg gcggcaatcg attcatttaa aggaagtgct
     1981 tcttcatttg aattaaatca ggcagttcta aatggttggg aagaccgtaa aggaagaaaa
     2041 atcaatgtgc cgattgcaga tggcggagaa aatactgcag aagcaatcta ttacgcgctc
     2101 gggggaacct ggcgtacgat tgatgggatg gatctcttat ttcgtcagcg aaaaatcagt
     2161 tatttattga cgaactggca aggaaaaaat accgcagtca ttgaatctgc acaagtatta
     2221 ggattagatt tacttgataa accaacggat caaaccatcc gacttgcttc tagttatgcc
     2281 ttgggagagc ttatcaaaga tacactgctt caagaagtgg aacaaatcat tgtcacttta
     2341 ggaggaagcg gatgtagcga tggtggtcta ggattactcc aatctttggg tgcttcttta
     2401 gaagggtaca cagaaggaaa tcctttgtta tctactagaa aacttcattt ggatcgaagg
     2461 aacgttcttg tttctaaagt aaaagtgatc atcgctgcag acgtgaccaa cccatataca
     2521 ggtcaacaag gagctgcttg gcaatttgga agacaaaaag gaggaacgga tacgacgtta
     2581 gctttcttgg ataaacaagc tgaaaagatt gctcagcaga taaaagatgt ttgggaggtg
     2641 gatttagatg acatcccagg aagcggagca gcaggcggta ttggcggagc cctttttttg
     2701 ttaggagcga agatggtctc tggttttgag ttggtcagcc aaatcactgg attagaaaaa
     2761 cagatcgaac aagcggatgt catcattaca ggagaagggc ggattgatca gcaaacaatc
     2821 cacggaaaag ttccatatgg cgtagcagtg cttgcaaaaa aatatcgaaa accaataatc
     2881 gctatttgcg gaagtagaga ccgagagctg gaaggactct ttgattttct ccccattgtc
     2941 ttaagcattc aattaggacc aatcgattta gaggaagcaa tggaacacag gcaaacatta
     3001 gaaaaaacag aaatcttagg acagacactc agcagatttt tcaacattct cccgaagtag
     3061 ttgacaaaaa caatttcttt atgctatcgt aatttcaaat ataggacaaa gtgagggatc
     3121 cgtgcattga aaaattaagt cgattgatga aagctgcatt cagactgtac cttgtggaga
     3181 caagggagca gtgtgcagat tttcgggatt ggcttggttc tttcgatgta tgggtctttt
     3241 tgtgtgcccc aacccttgca tcctctttag ccagttctga aaataggact ggtctttttt
     3301 tacgaggtga tggaataatg tctaaaatcg aaataaaaaa tctgacattc ggctatgaca
     3361 gccaaggcac attattattt gaacaagcca atctaaactt tgacacacaa tggaaactag
     3421 gacttatcgg acgaaacggt cgaggaaaga caactttact gaatatcctg caaaacaaac
     3481 tcccttatca agggcaagtg atccatcagc aagcgtttgt ctatttcccg cagcaaacaa
     3541 aagataaaga gcgtttgacc tattatgtgt taaatgagat tatggatttt gagatatggg
     3601 aaattgaaag agagctccaa ctgatgcaaa cagatcctga aatcttatgg agagaattca
     3661 gcacactatc aggaggagag aagacaaaag tcctactggc actcttattt gtggatgaca
     3721 ctcatttccc gttaatcgat gaaccaacga atcatttgga tatctctggc agaaaacaag
     3781 tagcggctta cttgaaaaag aaaaaacaag gattcatcgt ggtgagtcat gatcggggat
     3841 ttatcgatga agtagtggac catgttttag caatcgaaaa gagccaactg gaactttatc
     3901 aagggaattt ctctatctat gaagaacaga aaaaacttcg tgatgaattt gaaatagcta
     3961 aaaatgaaaa attgaaaaaa gaagtcagca ggctaaagaa aacagcagct gaaaaagcag
     4021 aatggtctcg ttcccgagaa ggggacaaaa caaagaaaaa agtcggattc atcgatacgg
     4081 aatctaggcg agtgaataaa ggagcagtag gcgctgatgc tgcacggacg atgaaacgat
     4141 ctaaagcaat cgtgaatcgg atggaaaccc agatcagtga gaaagaaaaa ttattaaaag
     4201 atatcgagta tatcgatgct ttgacgatga atagccaagt gtctcatcat aaacggctgt
     4261 taagcgtaga aaatcttcaa ttagggtatg aaaatctgtt attcgagcca gttcatttta
     4321 cgatcgaacc tcatcagcgg atggcaattt caggtcctaa cggcgcggga aagtcatcca
     4381 ttatccatta tcttctgggg acattcaacg gcaaggttgt aggagaaaaa tcccagccca
     4441 aacatttgac cattagttac gtacgccaaa attatgaaga caatcgaggg acattggcgg
     4501 aattcgcgaa gaaaaaccga gtagactatc aagcattttt gaataatctc cgaaagcttg
     4561 ggatggaaag agatgttttt cataataaaa tcgaacagat gagtatggga caacggaaaa
     4621 aagtggaatt ggctaaatct ttatcccaac cagctgagct ctatatatgg gatgaaccat
     4681 tgaattattt ggatgtcttc aatcaagaac aattagaaca actgattttg aacgtgaaac
     4741 ctgctatgtt actagtggaa catgatcaaa cctttctaga taaagtatct actgagatta
     4801 tttctcttga gaaaatctaa accttgtaat ctcaaagaca acaaggtatg gttaaggaga
     4861 actttgtcaa cgaaggagag aatcacatgc tttatgtaac gagacacgga gaaacaactt
     4921 ggaacgcaca aggattagtt tgcgggcgag cagatgtgcc gctgactgaa aaaggacaga
     4981 tgcaggcaca ggcactagca gaaaaggtag tagatttgcc tgttccaatc acgaaaatca
     5041 tccattcccc tcttcaacga gcaagagata cagcacaagc agtagttgat cgtctcaatt
     5101 tgccgatgac cgctgatgag cgattggtag aaatggattt tggggattat gacgg
//
LOCUS       DACEDD010000242         3443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.140, whole genome shotgun sequence.
ACCESSION   DACEDD010000242 DACEDD010000000
VERSION     DACEDD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.140"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..228
                     /gene="thyA"
                     /locus_tag="IWO45_003252"
     CDS             <1..228
                     /gene="thyA"
                     /locus_tag="IWO45_003252"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0334623.1"
                     /translation="HETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLN
                     QEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            248..775
                     /locus_tag="IWO45_003253"
     CDS             248..775
                     /locus_tag="IWO45_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0334624.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(838..1491)
                     /locus_tag="IWO45_003254"
     CDS             complement(838..1491)
                     /locus_tag="IWO45_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0334625.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1623..2465
                     /locus_tag="IWO45_003255"
     CDS             1623..2465
                     /locus_tag="IWO45_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0334626.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            2623..>3443
                     /locus_tag="IWO45_003256"
     CDS             2623..>3443
                     /locus_tag="IWO45_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0334627.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGY"
BASE COUNT         1260 a          618 c          670 g          895 t
ORIGIN      
        1 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
       61 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
      121 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
      181 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
      241 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
      301 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
      361 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
      421 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
      481 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
      541 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
      601 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
      661 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
      721 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
      781 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
      841 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
      901 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
      961 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     1021 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     1081 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     1141 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     1201 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     1261 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     1321 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     1381 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     1441 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     1501 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     1561 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     1621 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     1681 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     1741 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     1801 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     1861 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     1921 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     1981 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     2041 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     2101 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     2161 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     2221 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     2281 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     2341 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     2401 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     2461 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     2521 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     2581 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     2641 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     2701 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     2761 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     2821 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     2881 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     2941 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     3001 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     3061 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     3121 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     3181 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     3241 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     3301 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     3361 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     3421 catccaaata atttaggtta tca
//
LOCUS       DACEDD010000243         2856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.147, whole genome shotgun sequence.
ACCESSION   DACEDD010000243 DACEDD010000000
VERSION     DACEDD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.147"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWO45_003257"
     CDS             <1..395
                     /locus_tag="IWO45_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0334628.1"
                     /translation="EENIGISLTRYEIMMRLKEENPLLQSSLQEKLQIDQGAVTRHLK
                     VLEANGYVSRQRNPQNNREVYVALTEKAQKKIKHCEMDHKDTGSVLSPAFSDVEMKQF
                     LTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            379..1008
                     /locus_tag="IWO45_003258"
     CDS             379..1008
                     /locus_tag="IWO45_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0334629.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1362..2840
                     /gene="msr"
                     /locus_tag="IWO45_003259"
     CDS             1362..2840
                     /gene="msr"
                     /locus_tag="IWO45_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0334630.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         1010 a          473 c          606 g          767 t
ORIGIN      
        1 ttgaagaaaa tatcggtata agtcttacta gatacgagat catgatgaga ctgaaagaag
       61 aaaatccatt gctacaaagc agtttgcaag aaaagttgca gattgatcaa ggagcagtta
      121 ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc gcgtcagaga aatcctcaga
      181 acaaccggga agtgtatgta gcgctaacag aaaaagccca gaagaaaatc aagcattgtg
      241 aaatggacca taaagataca ggttccgtgc tatccccggc tttcagcgat gtagaaatga
      301 aacaatttct aacactttta gataaattga atacccaact ttgcaatata acaacagaaa
      361 cgaaagaggt taataataat ggaacaaaaa aatgattttt caacaattat gactggtaga
      421 agatccgttc gcgtatatga tgaaacatac aaaatcccac acgaagaaat gctggaaatg
      481 atcaaagaag cgacgactgc accttcttct gtcaatatga aaccatggcg ctttgtggtc
      541 gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga tccgtttcaa tacgagacaa
      601 aatgatactt cttctgcaat ggtcatgatt tttggcgata tgcaatgtta cgaatatggc
      661 gaagaaatct acaaccaagc ttacgaatca gggaagatgc caaaagaagt cagagatcaa
      721 caattagctg cgatcattcc ttattacaag agtttttcaa gagaagaaat gaacgatgta
      781 gtcaaagtcg attctagctt agcggctatg caattcatgt tagttgcgcg tgatcatggc
      841 tatgaaacaa atccaattgg tggattcgaa gcagatcagt tggcagaggc atttggtttg
      901 gataaagacc gttatgttcc agttattatt ttatcaatag gaaaagcggt agaagaaggt
      961 tacgagtctg ttcgtttagc accagaaaag atcactatgt tcgaataaaa ggttttcagt
     1021 tactattcag tagctattgc tttttttgaa tcgtgtgtgg taacctaata aaaaaggagg
     1081 tcggaaaaat gactgcatcg atgaaattac gtttcgaact tttgaataac aactaaataa
     1141 attcaaaaga catgtataaa caacaatttc atggatggag tatgtccatt ttttcgaacg
     1201 acagtagaat aggtgaaagc tatgacagat ttggtgattt agtcatagtt atttcgttca
     1261 gagtaggaaa agatgtgatg cagttttttt gcaaacagat ttttctgctc ttttttagta
     1321 tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa
     1381 atataacaaa tctacaagtc agctttggca accagctaga actatctatt gattctcttc
     1441 gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt aaatcgactt
     1501 tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa acagagatca
     1561 ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta aatttggaat
     1621 tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg agcggagggg
     1681 aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg atgcttctag
     1741 acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa gagcttcgtt
     1801 attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat gagctagcat
     1861 cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat tatagtgcct
     1921 atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag tcgatcatga
     1981 aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg
     2041 aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagact ggatcggttg tcttcctcta
     2101 aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta gagagaagac
     2161 tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt tttccagtac
     2221 caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc caattggaaa
     2281 gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg
     2341 cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc cgcaaacaag
     2401 gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa aagggttatc
     2461 aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag ttttcagaat
     2521 cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg acgaaaccgt
     2581 tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt tttactaagc
     2641 caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca acgatcgaag
     2701 ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct catgattctt
     2761 actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa ataaaaaaag
     2821 tacttacgag aaatttttaa atgtgctacg atgatt
//
LOCUS       DACEDD010000244         2856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.148, whole genome shotgun sequence.
ACCESSION   DACEDD010000244 DACEDD010000000
VERSION     DACEDD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.148"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWO45_003260"
     CDS             <1..395
                     /locus_tag="IWO45_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0334631.1"
                     /translation="EENIGISLTRYEIMMRLKEENPLLQSSLQEKLQIDQGAVTRHLK
                     VLEANGYVSRQRNPQNNREVYVALTEKAQKKIKHCEMDHKDTGSVLSPAFSDVEMKQF
                     LTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            379..1008
                     /locus_tag="IWO45_003261"
     CDS             379..1008
                     /locus_tag="IWO45_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0334632.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1362..2840
                     /gene="msr"
                     /locus_tag="IWO45_003262"
     CDS             1362..2840
                     /gene="msr"
                     /locus_tag="IWO45_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0334633.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKINKVLTRNF"
BASE COUNT         1009 a          473 c          606 g          768 t
ORIGIN      
        1 ttgaagaaaa tatcggtata agtcttacta gatacgagat catgatgaga ctgaaagaag
       61 aaaatccatt gctacaaagc agtttgcaag aaaagttgca gattgatcaa ggagcagtta
      121 ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc gcgtcagaga aatcctcaga
      181 acaaccggga agtgtatgta gcgctaacag aaaaagccca gaagaaaatc aagcattgtg
      241 aaatggacca taaagataca ggttccgtgc tatccccggc tttcagcgat gtagaaatga
      301 aacaatttct aacactttta gataaattga atacccaact ttgcaatata acaacagaaa
      361 cgaaagaggt taataataat ggaacaaaaa aatgattttt caacaattat gactggtaga
      421 agatccgttc gcgtatatga tgaaacatac aaaatcccac acgaagaaat gctggaaatg
      481 atcaaagaag cgacgactgc accttcttct gtcaatatga aaccatggcg ctttgtggtc
      541 gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga tccgtttcaa tacgagacaa
      601 aatgatactt cttctgcaat ggtcatgatt tttggcgata tgcaatgtta cgaatatggc
      661 gaagaaatct acaaccaagc ttacgaatca gggaagatgc caaaagaagt cagagatcaa
      721 caattagctg cgatcattcc ttattacaag agtttttcaa gagaagaaat gaacgatgta
      781 gtcaaagtcg attctagctt agcggctatg caattcatgt tagttgcgcg tgatcatggc
      841 tatgaaacaa atccaattgg tggattcgaa gcagatcagt tggcagaggc atttggtttg
      901 gataaagacc gttatgttcc agttattatt ttatcaatag gaaaagcggt agaagaaggt
      961 tacgagtctg ttcgtttagc accagaaaag atcactatgt tcgaataaaa ggttttcagt
     1021 tactattcag tagctattgc tttttttgaa tcgtgtgtgg taacctaata aaaaaggagg
     1081 tcggaaaaat gactgcatcg atgaaattac gtttcgaact tttgaataac aactaaataa
     1141 attcaaaaga catgtataaa caacaatttc atggatggag tatgtccatt ttttcgaacg
     1201 acagtagaat aggtgaaagc tatgacagat ttggtgattt agtcatagtt atttcgttca
     1261 gagtaggaaa agatgtgatg cagttttttt gcaaacagat ttttctgctc ttttttagta
     1321 tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa
     1381 atataacaaa tctacaagtc agctttggca accagctaga actatctatt gattctcttc
     1441 gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt aaatcgactt
     1501 tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa acagagatca
     1561 ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta aatttggaat
     1621 tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg agcggagggg
     1681 aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg atgcttctag
     1741 acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa gagcttcgtt
     1801 attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat gagctagcat
     1861 cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat tatagtgcct
     1921 atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag tcgatcatga
     1981 aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg
     2041 aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagact ggatcggttg tcttcctcta
     2101 aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta gagagaagac
     2161 tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt tttccagtac
     2221 caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc caattggaaa
     2281 gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg
     2341 cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc cgcaaacaag
     2401 gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa aagggttatc
     2461 aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag ttttcagaat
     2521 cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg acgaaaccgt
     2581 tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt tttactaagc
     2641 caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca acgatcgaag
     2701 ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct catgattctt
     2761 actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa ataaataaag
     2821 tacttacgag aaatttttaa atgtgctacg atgatt
//
LOCUS       DACEDD010000245         2657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.154, whole genome shotgun sequence.
ACCESSION   DACEDD010000245 DACEDD010000000
VERSION     DACEDD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.154"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..649
                     /locus_tag="IWO45_003263"
     CDS             <1..649
                     /locus_tag="IWO45_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0334634.1"
                     /translation="ADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGV
                     IGTGHIGQAVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTR
                     HLIGQKQIGEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTD
                     CSQKVLDHPFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            642..1670
                     /gene="vanB"
                     /locus_tag="IWO45_003264"
     CDS             642..1670
                     /gene="vanB"
                     /locus_tag="IWO45_003264"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0334635.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            1676..2284
                     /locus_tag="IWO45_003265"
     CDS             1676..2284
                     /locus_tag="IWO45_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAQ0334636.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          712 a          557 c          728 g          660 t
ORIGIN      
        1 tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca
       61 cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat
      121 gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg
      181 atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca
      241 gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga
      301 tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat
      361 caacaccgcg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg
      421 aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga
      481 ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt
      541 gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa
      601 aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg
      661 caatcatctt cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc gcaatagaaa
      721 ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg
      781 gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca
      841 tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa
      901 cacggcgtat tgatgtggct ttcccggttt tgcatggcaa atgcggggag gatggtgcga
      961 tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg
     1021 cagcttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc
     1081 ccgaatttca aattattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg
     1141 tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg
     1201 aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc
     1261 aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg
     1321 tcggcgaagt ggatcaaatc cggctgagcc acggtatctt ccgcatccat caggaaaacg
     1381 agccggaaaa aggctcagaa aatgcgatga ttacagttca cgcagacatt ccggtcgagg
     1441 aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc
     1501 ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata
     1561 ccatgcccgg ttttacatcg tacagccgct acccacgtat ggtggccgcc gcaggaatca
     1621 cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga
     1681 aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc
     1741 cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg
     1801 cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta
     1861 tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg
     1921 ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt taccccaata tagagcgagc
     1981 cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat
     2041 tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga
     2101 ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa
     2161 tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg
     2221 gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc
     2281 ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg
     2341 gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca aattatagta
     2401 ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc
     2461 cattttgtat tttgctggcg gcggttttga gaaatcaagg ccgccgttct tttctggcag
     2521 caaaaggtgt ggcggcccat aggaggatgc cgctatgctg ttttgctatt gccatgctct
     2581 aatccacttc caccactcaa aaaagccttg cccctccatg aactgaaccc caaaagttga
     2641 actatttaat ggactgt
//
LOCUS       DACEDD010000246         2514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.156, whole genome shotgun sequence.
ACCESSION   DACEDD010000246 DACEDD010000000
VERSION     DACEDD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.156"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..237
                     /locus_tag="IWO45_003266"
     CDS             <1..237
                     /locus_tag="IWO45_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0334637.1"
                     /translation="NGGKALDRAFSFLLWGNQEEERKSVHFCSINAKGMLIFIVADIW
                     NCYNMDMKKATNLKVLIIFKQPHCMEIRNRLSLK"
     gene            250..729
                     /locus_tag="IWO45_003267"
     CDS             250..729
                     /locus_tag="IWO45_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated DNA-protein cysteine
                     methyltransferase"
                     /protein_id="HAQ0334638.1"
                     /translation="MANEDKKDFNAMLHDSKDMPKFQIIIDQKSIEKYGGNKMYFAPP
                     IDYDKVMKKVPYGKVITVGKIREHFAKLSGADFTEPITAGIFVSIVAWASYQRSEDET
                     PYWRTLKANGELNAKYPNGIEAQKEKLEAEGHTIIQKGRKNIRYYVKDYENSLFDLK"
     gene            1037..1531
                     /gene="dfrF"
                     /locus_tag="IWO45_003268"
     CDS             1037..1531
                     /gene="dfrF"
                     /locus_tag="IWO45_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0334639.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKNELS
                     RFWI"
     gene            1615..2010
                     /locus_tag="IWO45_003269"
     CDS             1615..2010
                     /locus_tag="IWO45_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011068146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HAQ0334640.1"
                     /translation="MAISNINTIISNDIQKVWNIVLAVDKYNSWRSDLSKTEIINDKQ
                     FIEYTKNGYATTFTVTVAEPYKRWEFDMENSNMKGHWIGVFIDKGNETQIDFTENVIP
                     KKWFMKPFVKTYLKKQQKQFVLDLKKALE"
     gene            2037..2357
                     /locus_tag="IWO45_003270"
     CDS             2037..2357
                     /locus_tag="IWO45_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein TfoX"
                     /protein_id="HAQ0334641.1"
                     /translation="MASSKDYLQFVLEQLSELQEITYRAMMGEFIIYYRGKIVGGIYD
                     DRLLVKPTKSAISYMPTVTYEIPYENAKEMLLVEEVDNKDFLTGLFDVMYDELPTPKP
                     KKKK"
BASE COUNT          929 a          286 c          527 g          772 t
ORIGIN      
        1 aatggtggca aggctcttga cagggctttt tcttttctgt tgtggggtaa tcaagaggaa
       61 gaaagaaaaa gtgtgcattt ttgttccata aatgctaagg gtatgctgat ttttattgtg
      121 gcggatatat ggaactgtta taatatggat atgaagaaag cgacaaattt gaaagtgtta
      181 ataattttca aacagcctca ttgcatggaa ataagaaaca gactatcctt aaagtaggag
      241 gtggcacaaa tggcaaatga agataaaaaa gattttaatg ctatgttgca tgatagtaag
      301 gatatgccaa aatttcaaat tatcatagac cagaaaagta ttgagaaata tggtggaaac
      361 aaaatgtatt ttgctccccc gattgactat gacaaagtaa tgaaaaaagt tccgtatggt
      421 aaagtgatta cggttggaaa aatacgagaa cactttgcaa aattgagtgg tgcagatttt
      481 acagagccga ttacagcggg tatatttgtt tctattgtag cgtgggcgag ttatcagcgt
      541 tccgaagatg aaacacccta ttggagaaca ttaaaagcaa acggagaatt aaatgctaaa
      601 tatccaaatg gtattgaagc acagaaagaa aaattagaag cagagggaca taccattatt
      661 caaaaggggc gtaaaaatat acgatactat gtaaaggact atgaaaattc tctttttgat
      721 ttgaaatagg caaattctat ttcacaattt catatctcta tacacaaagc agttagtctt
      781 aggattgact gcttttttca tacctaaatt tcagattgga gtgatgaaat ggtgtttgtt
      841 agagctgttc agtaagatta tcggtgcata aaattttatt gtggtgcaga tatgaaaatg
      901 atataatgtg gatatgaaga aagcgacaaa cttgaatttg tcgagctggt tagttcgaga
      961 taattttgag tttgttaaga tgtggtgcac ttgatggaat cacaggtacg acatcatatt
     1021 atggaggatg aaatagatga taggtttgat tgttgcgagg tcaaagaata atgttatagg
     1081 caagaatggt aatataccat ggaaaataaa gggagaacaa aagcaattta gagagttaac
     1141 aacgggtaat gtggttatta tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc
     1201 taatagaatg aatattgttg tttccaccac aacagagtat caaggagata atttagtttc
     1261 agttaaatca ttagaagatg cattattatt ggctaaagga cgagatgtat acatatctgg
     1321 tggatatgga ctatttaagg aagctttgca aatagtagat aaaatgtata tcacagaagt
     1381 agatttaaat attgaagatg gagatacatt ctttccagaa tttgatatca atgattttga
     1441 agttttgata ggggaaacac ttggtgagga agtgaaatat acgagaacat tttatgtaag
     1501 gaaaaatgaa ttgagtagat tttggattta gggtgttttc ataaatatat tgctttttat
     1561 gcatatataa attgttgtaa gacaaattcc agtttgaaaa ggggaaatga aaaagtggca
     1621 atttcaaata taaatacaat tattagtaat gatattcaaa aagtttggaa cattgtttta
     1681 gctgttgata aatataactc atggcgaagt gatttgagca aaacagaaat aataaatgac
     1741 aaacaattta ttgaatacac aaaaaatgga tatgctacta catttaccgt taccgttgca
     1801 gaaccatata aacgctggga atttgatatg gagaatagca acatgaaagg tcattggatt
     1861 ggtgttttta tagacaaagg gaatgaaaca caaatagact ttacagaaaa tgtgatacct
     1921 aaaaagtggt ttatgaaacc ttttgtaaaa acctatttga aaaaacaaca aaagcaattt
     1981 gttctggact taaagaaagc attggaataa taaaattcaa gattggaggg ttggaaatgg
     2041 cttcaagtaa agattattta caatttgttt tagaacaatt atcagaatta caagaaataa
     2101 catatcgtgc aatgatggga gaatttatta tttattatcg tggaaaaatc gttggcggta
     2161 tatatgatga taggttactt gtaaaaccaa caaagtctgc aatttcttat atgccgacag
     2221 ttacttatga aattccatac gaaaacgcca aagaaatgtt attggtggaa gaagttgata
     2281 ataaagattt tctgacgggg ttatttgatg tgatgtatga tgaactacca acgccaaaac
     2341 ctaaaaagaa aaaataaaca gcttctagtt tacaatttca tatccatata cacaaagcag
     2401 ttagttttag gattgactgc tttttaaaaa gacaccattt aggtgtaatg atgtcttttg
     2461 ggtgttattt tataggtcat atcaaggctc attcaatcgt ggtaaattcg tggt
//
LOCUS       DACEDD010000247         2369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.163, whole genome shotgun sequence.
ACCESSION   DACEDD010000247 DACEDD010000000
VERSION     DACEDD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.163"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003271"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0334642.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWO45_003272"
     CDS             2039..2227
                     /locus_tag="IWO45_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0334643.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="IWO45_003273"
     CDS             complement(2285..>2369)
                     /locus_tag="IWO45_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0334644.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          820 a          361 c          460 g          728 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
      661 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
      721 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
      781 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
      841 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DACEDD010000248         2369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.164, whole genome shotgun sequence.
ACCESSION   DACEDD010000248 DACEDD010000000
VERSION     DACEDD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.164"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003274"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0334645.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWO45_003275"
     CDS             2039..2227
                     /locus_tag="IWO45_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0334646.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="IWO45_003276"
     CDS             complement(2285..>2369)
                     /locus_tag="IWO45_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0334647.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          819 a          361 c          461 g          728 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
      661 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
      721 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
      781 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
      841 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DACEDD010000249         2339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.166, whole genome shotgun sequence.
ACCESSION   DACEDD010000249 DACEDD010000000
VERSION     DACEDD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.166"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..1018
                     /locus_tag="IWO45_003277"
     CDS             47..1018
                     /locus_tag="IWO45_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAQ0334648.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="IWO45_003278"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="IWO45_003278"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAQ0334649.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..>2339
                     /locus_tag="IWO45_003279"
     CDS             2045..>2339
                     /locus_tag="IWO45_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HAQ0334650.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPN"
BASE COUNT          622 a          493 c          670 g          554 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaata
//
LOCUS       DACEDD010000250         2031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.169, whole genome shotgun sequence.
ACCESSION   DACEDD010000250 DACEDD010000000
VERSION     DACEDD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.169"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003280"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0334651.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          700 a          312 c          421 g          598 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg a
//
LOCUS       DACEDD010000251         2031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.170, whole genome shotgun sequence.
ACCESSION   DACEDD010000251 DACEDD010000000
VERSION     DACEDD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.170"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003281"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0334652.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          703 a          305 c          412 g          611 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
      661 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
      721 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
      781 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
      841 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg a
//
LOCUS       DACEDD010000252         2031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.171, whole genome shotgun sequence.
ACCESSION   DACEDD010000252 DACEDD010000000
VERSION     DACEDD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.171"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003282"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0334653.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          699 a          312 c          422 g          598 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg a
//
LOCUS       DACEDD010000253         2031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.172, whole genome shotgun sequence.
ACCESSION   DACEDD010000253 DACEDD010000000
VERSION     DACEDD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.172"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003283"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWO45_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0334654.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          702 a          305 c          413 g          611 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
      661 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
      721 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
      781 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
      841 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg a
//
LOCUS       DACEDD010000254         1860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.179, whole genome shotgun sequence.
ACCESSION   DACEDD010000254 DACEDD010000000
VERSION     DACEDD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.179"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..654
                     /locus_tag="IWO45_003284"
     CDS             <1..654
                     /locus_tag="IWO45_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0334655.1"
                     /translation="AGEWETLKKMLPDFKGKRVLDLGCGYGWHCIYAMENGASSVVGV
                     DISHKMLEVAKGKTHFPQIEYECCAIEDVDFPEESFDVILSSLAFHYVADYENLIKKI
                     YRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPVDNYYYEGKRTAMFLEEKV
                     TKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            687..1595
                     /locus_tag="IWO45_003285"
     CDS             687..1595
                     /locus_tag="IWO45_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0334656.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1592..1840
                     /locus_tag="IWO45_003286"
     CDS             1592..1840
                     /locus_tag="IWO45_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0334657.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT          647 a          263 c          424 g          526 t
ORIGIN      
        1 gcgggagaat gggagacttt gaaaaagatg ctacctgatt ttaagggtaa gcgtgtgctt
       61 gatttaggat gcggctatgg atggcactgt atatatgcga tggaaaacgg tgcttcctct
      121 gtagtaggtg ttgatatttc tcataaaatg ctcgaagtag caaaaggaaa aacccatttt
      181 ccacagattg aatatgaatg ctgtgccata gaagatgtgg atttcccaga ggagagcttt
      241 gatgtaatac taagttcgct tgcgtttcat tatgtagcag actatgagaa tttaataaaa
      301 aagatatata ggatgctgaa ggctggtggc aatttagttt ttacagttga acatcctgtt
      361 tttactgctc atggaacaca agactggtat tataacgaaa aaggagaaat actgcatttc
      421 ccggtggaca attattatta tgagggcaaa cggacagcta tgtttttgga agaaaaggtt
      481 acaaaatatc atagaacact gaccacatat ctaaatacac tgctttcaaa tagttttata
      541 ataaatcaga ttgtggagcc acagccgcca gagaacatga tggatattcc ggggatggcg
      601 gatgaaatgc gacgcccaat gatgctgatt gtatcggcaa aaaagaagat gtaataatat
      661 agaaaaaata aacgaggagt atgtaaatga gatcagaaaa agaaatgatg gatttagtac
      721 tttctttagc agaacaggat gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa
      781 atattaatat acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata
      841 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
      901 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
      961 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
     1021 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
     1081 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
     1141 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
     1201 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
     1261 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
     1321 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
     1381 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
     1441 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
     1501 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
     1561 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
     1621 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
     1681 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     1741 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
     1801 tgataagata atatatcttt taacccgtgg tgctagttaa ttttaatatc ctaaagaata
//
LOCUS       DACEDD010000255         1847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.180, whole genome shotgun sequence.
ACCESSION   DACEDD010000255 DACEDD010000000
VERSION     DACEDD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.180"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            48..1421
                     /gene="tet(L)"
                     /locus_tag="IWO45_003287"
     CDS             48..1421
                     /gene="tet(L)"
                     /locus_tag="IWO45_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0334658.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          531 a          273 c          374 g          669 t
ORIGIN      
        1 tattttttaa taaagatttg aattaaagtg taaaggagga attaattatg aagtgtaatg
       61 aatgtaacag ggttaaatac aaccaaattt taatttggct ttgcgtttta tcttttttta
      121 gcgtattaaa tgaaatggtt ttgaacgtct cattacctga tattgcaaat gattttaata
      181 aaccacctgc gagtacaaac tgggtgaaca cagcctttat gttaaccttt tccattggaa
      241 cagctgtata tggaaagcta tctgatcaat taggcatcaa aaggttactc ctatttggaa
      301 ttataataaa ttgtttcggg tcggtaattg ggtttgttgg ccattctttc ttttccttac
      361 ttattatggc tcgttttatt caaggggctg gtgcagctgc atttccagca ctcgtaatgg
      421 ttgtagttgc gcgctatatt ccaaaggaaa ataggggtaa agcatttggt cttattggat
      481 cgatagtagc catgggagaa ggagtcggtc cagcgattgg tggaatgata gcccattata
      541 ttcattggtc ctatcttcta ctcattccta tgataacaat tatcactgtt ccgtttctta
      601 tgaaattatt aaagaaagaa gtaaggataa aaggtcattt tgatatcaaa ggaattatac
      661 taatgtctgt aggcattgta ttttttatgt tgtttacaac atcatatagc atttcttttc
      721 ttatcgttag cgtgctgtca ttcctgatat ttgtaaaaca tatcaggaaa gtaacagatc
      781 cttttgttga tcccggatta gggaaaaata taccttttat gattggagtt ctttgtgggg
      841 gaattatatt tggaacagta gcagggtttg tctctatggt tccttatatg atgaaagatg
      901 ttcaccagct aagtactgcc gaaatcggaa gtgtaattat tttccctgga acaatgagtg
      961 tcattatttt cggctacatt ggtgggatac ttgttgatag aagaggtcct ttatacgtgt
     1021 taaacatcgg agttacattt ctttctgtta gctttttaac tgcttccttt cttttagaaa
     1081 caacatcatg gttcatgaca attataatcg tatttgtttt aggtgggctt tcgttcacca
     1141 aaacagttat atcaacaatt gtttcaagta gcttgaaaca gcaggaagct ggtgctggaa
     1201 tgagtttgct taactttacc agctttttat cagagggaac aggtattgca attgtaggtg
     1261 gtttattatc cataccctta cttgatcaaa ggttgttacc tatggaagtt gatcagtcaa
     1321 cttatctgta tagtaatttg ttattacttt tttcaggaat cattgtcatt agttggctgg
     1381 ttaccttgaa tgtatataaa cattctcaaa gggatttcta aatcgttaag ggatcaactt
     1441 tgggagagag ttcaaaattg atcctttttt tataacagga attcaaatct ttttgttcca
     1501 ttaaagggcg cgattgctga ataaaagata cgagagacct ctcttgtatc ttttttattt
     1561 tgagtggttt tgtccgttac actagaaaac cgaaagacaa taaaaatttt attcttgctg
     1621 agtctggctt tcggtaagct agacaaaacg gacaaaataa aaattggcaa gggtttaaag
     1681 gtggagattt tttgagtgat cttctcaaaa aatactacct gtcccttgct gatttttaaa
     1741 cgagcacgag agcaaaaccc ccctttgctg aggtggcaga gggcaggttt ttttgtttct
     1801 tttttctcgt aaaaaaaaga aaggtcttaa aggttttatg gttttgg
//
LOCUS       DACEDD010000256         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.186, whole genome shotgun sequence.
ACCESSION   DACEDD010000256 DACEDD010000000
VERSION     DACEDD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.186"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWO45_003288"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWO45_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWO45_003289"
     CDS             394..477
                     /locus_tag="IWO45_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0334659.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO45_003290"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO45_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0334660.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACEDD010000257         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.191, whole genome shotgun sequence.
ACCESSION   DACEDD010000257 DACEDD010000000
VERSION     DACEDD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.191"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            315..812
                     /gene="dfrG"
                     /locus_tag="IWO45_003291"
     CDS             315..812
                     /gene="dfrG"
                     /locus_tag="IWO45_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0334661.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(884..>1417)
                     /locus_tag="IWO45_003292"
     CDS             complement(884..>1417)
                     /locus_tag="IWO45_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0334662.1"
                     /translation="LLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEEAISKDGERY
                     RKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRY
                     ISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQR
                     LGITDKQFDWKDIIYFK"
BASE COUNT          496 a          185 c          270 g          466 t
ORIGIN      
        1 gtaaatgtag tactccacta taaaggcgta tatatgcaag taagtagttc agttttggag
       61 tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac ttacatacca
      121 aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat gaaaatttgt
      181 gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca actaacgggc
      241 aggataatga gaaaaggttt tttgatagaa ataagaaatt agatggtaaa ttagttaaaa
      301 aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat ggataagaat agagtgattg
      361 gcaaagagaa tgacattcct tggaggattc ccaaggactg ggaatatgtt aaaaatacta
      421 caaagggaca tccgataata ttaggtagga agaaccttga atcaatcgga agagccttac
      481 ctgacagaag aaatattatt ctgacgagag ataaggggtt tacctttaat ggttgtgaaa
      541 ttgttcattc aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa atttttattt
      601 tcggaggaga acagatttat aatttgtttt tcccttatgt tgagaaaatg tacatcacaa
      661 aaatacatca tgaattcgaa ggagatactt tttttccaga agtgaattat gaggaatgga
      721 atgaggtatt tgcccaaaaa gggataaaga atgataaaaa tccgtataac tactattttc
      781 atgtatatga aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag aagaacagta
      841 aagataaagc acaagagctt gggattactc caagctcttt ttgtcatttg aaataaatta
      901 tatctttcca atcaaattgt ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct
      961 ttttatccgc tgatttaaaa gggcgacaaa agttgtagta agttcttaga attgtaatcg
     1021 caaattgagc atacttcgga ttgaagttag cgtaaatata gctcttctta tcaccacgag
     1081 ctgttgttaa tggtctttca agagatgata tataacgtct tatctgttga ataaagctat
     1141 tggtcgaatg gtcattaaca tttaatagca tctttgcgat ttcgtttggt tccagagcag
     1201 ataaatccgt tctacaatca actgaataaa atcctttatc cttagtggct aatggatgtt
     1261 tgatagggtt atcggcatat ttgcgatatc tttctccatc tttactaata gcctcttcgt
     1321 ggaattgatg acttgttagt aattcagtta gataatgaga tgccaaagtc cgtagaaact
     1381 ttgttttgaa tcctctgata tctccccaat cgagcag
//
LOCUS       DACEDD010000258         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.194, whole genome shotgun sequence.
ACCESSION   DACEDD010000258 DACEDD010000000
VERSION     DACEDD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.194"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            45..296
                     /locus_tag="IWO45_003293"
                     /pseudo
     CDS             45..296
                     /locus_tag="IWO45_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            389..1183
                     /locus_tag="IWO45_003294"
                     /note="aph(3')-IIIa"
     CDS             389..1183
                     /locus_tag="IWO45_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0334663.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          233 c          322 g          369 t
ORIGIN      
        1 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
       61 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      121 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      181 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      241 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      301 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      361 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      421 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      481 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      541 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      601 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      661 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      721 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      781 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      841 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      901 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
      961 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1021 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1081 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1141 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1201 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1261 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1321 gcctacgagg aatttgtatc gataagaa
//
LOCUS       DACEDD010000259         1267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.199, whole genome shotgun sequence.
ACCESSION   DACEDD010000259 DACEDD010000000
VERSION     DACEDD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.199"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            266..1141
                     /locus_tag="IWO45_003295"
                     /note="aph(2'')-Ia"
     CDS             266..1141
                     /locus_tag="IWO45_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0334664.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          541 a          101 c          206 g          419 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt acaagatata ttaaattgat
       61 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      121 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      181 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      241 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
      301 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
      361 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
      421 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
      481 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      541 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      601 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      661 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      721 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      781 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      841 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      901 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      961 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
     1021 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
     1081 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
     1141 attatataat atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat
     1201 gaaaaataat aaaggaagtg agtcaattcc agactcctgt gtaaaatgct atacaatgtt
     1261 tttacca
//
LOCUS       DACEDD010000260         1079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.209, whole genome shotgun sequence.
ACCESSION   DACEDD010000260 DACEDD010000000
VERSION     DACEDD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.209"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            466..1014
                     /gene="aac(6')"
                     /locus_tag="IWO45_003296"
     CDS             466..1014
                     /gene="aac(6')"
                     /locus_tag="IWO45_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0334665.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          392 a          158 c          210 g          319 t
ORIGIN      
        1 cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta
       61 tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct
      121 tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag
      181 attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa
      241 gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat
      301 ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag taagataaat
      361 aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt
      421 tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa
      481 tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag actgacttgg
      541 ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga
      601 atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa
      661 tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac
      721 caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc
      781 acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg
      841 tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa
      901 ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac
      961 aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa
     1021 gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACEDD010000261         1032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41105_2017
            SAMN12640068-rid9879793.guided.212, whole genome shotgun sequence.
ACCESSION   DACEDD010000261 DACEDD010000000
VERSION     DACEDD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640068
            Sequence Read Archive: SRR10023217
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741742.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741742.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:57:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,240
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 37 of 87
            Pseudo Genes (incomplete)         :: 41 of 87
            Pseudo Genes (internal stop)      :: 22 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640068-rid9879793.guided.212"
                     /isolate="E41105_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="IWO45_003297"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="IWO45_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAQ0334666.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            194..928
                     /locus_tag="IWO45_003298"
     CDS             194..928
                     /locus_tag="IWO45_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ0334667.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          415 a          136 c          140 g          341 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaacctcata
      181 ggagtgggtt ataatgaaca aaaaaaatat aaaagatagt caaaacttta ttacttcaaa
      241 gcatcatata aatgaaattt tgagaaatgt acatttaaat acaaatgata atattattga
      301 gattggttca gggaaaggtc atttcacgtt tgaattagct aaaaggtgta attatgtaac
      361 cgccattgaa atagatccta aattatgtag gataactaaa aacaaactta ttgaatatga
      421 gaacttccag gttatcaata aagatatttt acaatttaag tttcctaaaa ataagtcata
      481 taagatatat ggaaatatac cctacaatat aagtacagat ataattcgaa aaattgtttt
      541 tgaaagcaca gctacagaaa gttatttaat agtggaatat ggatttgcta aaaggttgct
      601 aaatacaaat cgttcactag cactattttt aatgacagaa gttgatatat ccatattaag
      661 taaaatccct agagaatact ttcatccaaa acctagagtt aatagctcgt taattgtatt
      721 aaaaagacac ccttcaaaaa tatcactcaa agatagaaaa caatatgaaa attttgttat
      781 gaaatgggtt aacaaagaat acataaaact attttccaaa aaccaatttt atcaagcctt
      841 aaaatatgca agaattgacg atttaaacaa tattagcttt gaacaattct tgtctctttt
      901 caatagctat aaattattta atagataagt taaggggtgc ataaactgca tcccttaact
      961 tatcttattt tgcttaaaca atcttggccc gtaaactgca taactcatat gtaaatcgct
     1021 cctttttagg tg
//
